Digital Commons Network^™

And This, Our Measure: Figurative Realism And Workaday Culture., Perry Walter Johnson

Electronic Theses and Dissertations

Man has created vast and powerful systems. These systems and their associated protocols, dogmas, and conventions tend to limit rather than liberate. Evaluation based on homogeneity threatens the devaluation of our humanity. This paper and the artwork it supports examine contemporary culture and its failings in the stewardship of the humanist ideal.

My paintings referenced herein are satirical narratives. The story told is one of alienation and evisceration. The visual subject matter is the human figure and environments dominated by architectural geometry. The male figures are conspicuously turned from view, a visual cue to their estrangement. Parallel and subordinate thoughts …

Light Image Therapy In The Health Care Environment., Anna Rae Dutro

Electronic Theses and Dissertations

Use of positive distraction in the built healthcare environment to assist in alleviating stress in a patient was investigated. A backlit light image was mounted in the ceiling of an examination room to create a positive distraction for patients in the ETSU Pediatric Clinic in Johnson City, TN. Survey instruments were used to collect sample data from patients and physicians in a randomized, balanced controlled study designed to determine if patients experienced less stress in the room with the backlit image as compared to other rooms (treatments). Although a statistical difference was not determined between the room with the backlit …

1, Structural And Functional Studies Of Human Replication Protein A; 2 Dna Damage Responses And Dna Repair Defects In Laminopathy-Based Premature Aging., Yiyong Liu

Electronic Theses and Dissertations

The genome of mammalian cells is under constant attack from DNA-damaging agents. To maintain genomic integrity, cells activate an array of pathways primarily consisting of DNA repair and DNA damage checkpoints. Human replication protein A (RPA), a single-stranded DNA (ssDNA) binding protein, is essential for almost all DNA metabolic pathways. However, the role of RPA in nucleotide excision repair (NER), a DNA repair pathway for removing bulky DNA lesions, remains elusive. In this study, the binding of RPA to a battery of well-defined ssDNA substrates has been systematically examined using fluorescence spectroscopy. The results showed that RPA has a lower …

Lost Cove, North Carolina: The Life And Death Of A Thriving Community (1864-1957)., Christy A. Smith

Electronic Theses and Dissertations

This research examines the history and events that shaped the people and community of Lost Cove, an isolated Appalachian settlement. Chapter 1 surveys previous written and oral accounts of Lost Cove and the physical/cultural attributes of the community. Chapter 2 explores Lost Cove's identity, name, and first settlers. Chapter 3 explores the community's buildings and the families' livelihood. Chapter 4 examines the effect that the CC & O Railway and the sawmills had on the community. Chapter 5 examines moonshine selling in Lost Cove. Chapter 6 reveals how the church and school acted as a gathering place and how sermons …

Localization Of A Microsporidia Adam (A Disintegrin And Metalloprotease Domain) Protein And Identification Of Potential Binding Partners., Carrie E. Jolly

Electronic Theses and Dissertations

Microsporidia are spore-forming, obligate intracellular pathogens typically associated with opportunistic infections in immunocompromised individuals. Treatment options for microsporidia infections in humans are limited and additional research is necessary to create better therapeutic agents. For many pathogenic organisms, adhesion to the host cell surface is a prerequisite for tissue colonization and invasion. Our previous research has demonstrated a direct relationship between adherence of microsporidia spores to the surface of host cells and infectivity in vitro. In an effort to better understand adherence, we have turned our attention to determining what proteins may be involved in this process. Examination of the …

Design And Construction Of The Audrey Ii Puppet Series For The Play Little Shop Of Horrors., Chad David Fraley

Electronic Theses and Dissertations

This project's objective was to design and build 4 stages of the Audrey II puppet for the East Tennessee State University production of Little Shop of Horrors (Ashman, 1982). The puppets were to be created with consideration for the production space, budget, and operator ease, along with audience impact. This paper will present the design and construction in a step by step-bystep fashion using illustrations and photographic examples.

Each puppet in the series was originally conceived using references to living plants and animals combined with an artistic vision of the designer. The puppets were built with light, modern materials using …

From Teacher To Teller: How Applied Storytelling Informs Autobiographical Instruction., Peggy Rosann Kent

Electronic Theses and Dissertations

This thesis uses autobiographical inquiry to "re-member" how I came to understand that applied storytelling was a valid teaching tool in facilitating autobiographical expression in mature learners. It is an examination of how story sharing and story listening can transform a continuing education classroom into a learning community. Applied storytelling can help elders reframe their negative mental models about the value of their stories, memory, and mythology and create opportunities for positive story sharing experiences. I selected highlights of my journey that best represented my experience and use of applied storytelling techniques. Each chapter includes an exercise and reflection as …

Probability And Statistics For Third Through Fifth Grade Classrooms., Melissa Taylor Mckinnon

Electronic Theses and Dissertations

This document contains a variety of lesson plans that can be readily used by a teacher of intermediate students. This thesis contains two units in Probability and one unit in Statistics. Any educator can supplement this document with any curriculum to teach lessons from vocabulary to concept.

Principal Leadership And Its Perceived Influence On Teacher Morale In Elementary Schools., Anne Runyan Littleford

Electronic Theses and Dissertations

The purpose of this qualitative study was to construct a theoretical framework explaining the connection between principal leadership practices and the phenomenon of teacher morale. The study also focused on principal leadership practices and the perceived effects that style has on how teachers feel about themselves and their jobs. First, a review of extant literature defined what constitutes effective leadership in school related environments. Second, the researcher defined teacher morale, both high and low, and how it relates to work performance. Third, the researcher attempted to explore how teacher morale and the leadership style of their principals relate.

This qualitative …

Integration Of Fifth Grade Math And Science Curriculum, Accompanied By Increased Parental Involvement, Produces Higher Virginia Test Scores., Kathy Diane Perkins

Electronic Theses and Dissertations

The National Educational Society, through much research and testing, discovered that American students are not performing as well on the academic level as their counterparts. As a nation the math and science scores fell behind other tested disciplines. The Virginia Department of Math and Science Report Card scores confirm that students are struggling in these areas.

As a resolution to correct this problem a proposal for the integration of fifth grade math and science curriculum accompanied by increased parental involvement was devised. The program involved thirteen elementary students and their parents. Pretesting, math and science labs, worksheets, posttesting, and a …

"Reclaiming The Child": Mountain Mission School As A Successful Appalachian Home Mission., Rachel Rebecca Hood

Electronic Theses and Dissertations

Mountain Mission School of Grundy, Virginia, founded by Samuel Robinson Hurley in 1921, is an anomaly of the mission school era of 1880 to 1940. Unlike other mission schools, Mountain Mission School was independent from its inception and was founded by a self-taught, self-made millionaire from southwest Virginia. The school's purpose to "reclaim" the child from material and spiritual poverty lay in Hurley's desire to develop a child's mind, body, and soul through a Christian, industrial education. Through personal commitment to the school and tireless fund-raising efforts for the school, he inspired others to continue the mission he began. Primary …

My Journey Home: A Study In Hand-Felted Wool., Ashleigh Robertson

Electronic Theses and Dissertations

This is a supporting paper for a Master of Arts Graduate Exhibition entitled My Journey Home: A Study in Hand-Felted Wool, held at Slocumb Galleries October 30 through November 3, 2006. The work in the exhibition represents a concentrated study in the graduate program in Fiber Art at East Tennessee State University. My artwork is an expression of my feelings and emotions toward my family, particularly the women in my family.

The paper begins with an introduction detailing my artistic research and culminates with a reflection of the work shown in the exhibition. The cultural and historical influences that shaped …

Finite Element Modeling Of Soft Tissue Deformation., Hongjian Shi

Electronic Theses and Dissertations

Computer-aided minimally invasive surgery (MIS) has progressed significantly in the last decade and it has great potential in surgical planning and operations. To limit the damage to nearby healthy tissue, accurate modeling is required of the mechanical behavior of a target soft tissue subject to surgical manipulations. Therefore, the study of soft tissue deformations is important for computer-aided (MIS) in surgical planning and operation, or in developing surgical simulation tools or systems. The image acquisition facilities are also important for prediction accuracy. This dissertation addresses partial differential and integral equations (PDIE) based biomechanical modeling of soft tissue deformations incorporating the …

The Changes Of Fructos-2,6-Bisphosphate Level In Transgenic Mice Causing Cardiomyopathy., Jianxun Wang 1969-

Electronic Theses and Dissertations

Heart failure is recognized as a major cause of death among diabetics. In type I and II diabetes, glucose uptake, glycolysis and pyruvate oxidation are impaired, and fatty acid utilization increases. These alterations in metabolism contribute toward cardiac contractile function. There is a gap in our understanding on how alterations in glycolytic metabolite fructose-2,6-bisphosphate (F-2,6-P 2 ) affects cardiac dysfunction. Two cardiac-specific overexpression of PFK-2 (phosphofructose kinase-2) transgenic models were used to investigate the role of altered metabolism by F-2,6-P 2 in provoking cardiomyopathy. One model is with the PFK-2 kinase active and phosphatase inactive enzyme called MK and another …

Therapeutic Targeting Of Tumor Associated Macrophages., Stephanie Kaye Watkins 1981-

Electronic Theses and Dissertations

Previous studies on the mechanistic induction of anti-tumor responses by IL-12 cytokine therapy have focused on the adaptive immune response, specifically the activation NK cells and T cells as the primary targets of IL-12 treatment. In contrast, little attention has been given to the potential role of macrophages in the initiation of anti-tumor responses by IL-12 therapy despite reports that macrophages play a major role in promoting tumor growth and metastasis and in suppressing anti-tumor immune responses. Based on the functional adaptivity hypothesis, which is the concept that macrophages functionally adapt, rather than differentiate into specific mature subsets, in response …

Molecular Interactions Between The Wilms' Tumor Transcription Factor And The Aryl Hydrocarbon Receptor During Nephrogenesis And Its Impact On Fetal Programming Of Renal Disease., Adrian Nanez

Electronic Theses and Dissertations

Embryonic development requires the orchestration of temporally precise genetic events that culminate in the formation of a complete organism. The molecular mechanisms responsible for ontogenesis are regulated by environmental and somatic factors in utero that activate or repress the expression of numerous genetic elements resulting in fetal programming of adult diseases. The aryl hydrocarbon receptor (AHR) is an important nuclear transcription factor both during embryogenesis and throughout maturity in multiple organisms. Building upon interesting studies establishing a direct, novel link between AHR and post-transcriptional regulation of the Wilms' tumor suppressor ( WT1 ) gene, the overall goal of the project …

Gene Therapy And Cellular Transplantation : Towards A Combination Strategy For Spinal Cord Injury Repair., Ahmed Abdelzaher Abdellatif

Electronic Theses and Dissertations

The combination of viral mediated gene delivery, tissue engineering, and Schwann cell (SC) transplantation offer a promising strategy to enhance axonal regeneration and functional recovery following spinal cord injury (SCI). The rationale and feasibility of using such combination has been extensively reviewed in Chapter 1. The study presented in Chapter 2 showed that lentiviral vectors are most suitable in situations where stable long term transgene expression is needed, while adenovirus and retrovirus are more suited for transient gene delivery. Tissue engineering combined with Schwann cell (SC) transplantation is a promising method to enhance regeneration and remyelination of axons following a …

Effects Of Direct Atp Delivery To Ischemic Skeletal Muscle., Federico V. Grossi 1974-

Electronic Theses and Dissertations

The purpose of this dissertation was to demonstrate that a new method of direct intracellular energy delivery was effective in maintaining viability of in vitro Human Umbilical Vein Endothelial Cells (HUVECs) when exposed to chemical ischemia for prolonged periods of time and in preventing in vivo ischemia reperfusion injury in a composite tissue transplantation model. Ischemia and reperfusion injury is a well recognized phenomenon that occurs in situations like organ transplantation, shock, cardiac surgery, etc, where tissues are temporarily deprived of nutrients and oxygen for energy production. When exposed to short periods of ischemia, cells utilize their stored energy to …

André Jeunet : Expanded Educational Opportunities For A Photographic Exhibition., Brian J. Davis 1958-

Electronic Theses and Dissertations

In spring 2006, the Frazier International History Museum, in conjunction with the Ekstrom Library Photographic Archives at the University of Louisville, mounted an exhibit of 47 photographs taken by a young French foot soldier during World War I. The exhibit, titled "André Jeunet: Images of WWI by a Pacifist Soldier," ran from May 19 through September 4, 2006. The main concept behind the exhibit was to show images of World War I that focused less on the horrific aspects of combat and more of the everyday life the of common soldier, and to highlight that although many soldiers fought out …

Soil Phosphate Detection And Archaeology : In-Stride Phosphate Detection And The Elimination Of Arsenate Interference To The Malachite Green Method., Laura A. Deneve

Electronic Theses and Dissertations

Archaeologists use soil analysis to detect chemicals, like phosphate, to indicate areas of anthropogenic activity. Phosphate detection is a multi-step process, which makes standard techniques time consuming. Kinetic studies decreased the analysis time for the malachite green (MG) method of phosphate detection. The 3-minute method allows extraction and analysis to be complete in 15 minutes. Continued studies resulted in two-color spectral monitoring, which provided values instantaneously. Arsenate (As(V)) interfere with the MG method and results in overestimation of phosphate. As(V) must be reduced to non-interfering arsenite. Two As(V) reducing agents--L-Cysteine and thiosulfate--were investigated. The thiosulfate method was suitable for field …

Design Of An Active Orthotic Device For Joint Management., Steven Clayton Anderson

Electronic Theses and Dissertations

Existing treatment programs and procedures are incapable of addressing the complications encountered with patients who experience spasticity and hypertonia related joint contractures. Current passive therapy procedures and devices are only capable of managing the extent of joint contractures on patients with chronic and acute onset severe neurological disorders. The project was conceived as a means to develop an active device that is capable of adapting to the state of the joint to mange the extent of contractures and to permit consideration for the prevalence of spastic activity episodes and hypertonia. The project focused on the design of the physical prototype …

The Function Of Mythology And Religion In Ancient Greek Society., Cara Leigh Sailors

Electronic Theses and Dissertations

The ancient Greeks are prime subjects of study for those wishing to understand the roles that religion and mythology play in a society and how the two interact with each other. This paper covers what I feel after my study of Greek mythology and religion are the eight functions of mythology: history, education, explanation - both of the natural world and the culture of each society, legality, genesis, what happens after death, and entertainment; as well as the two function of religion: civic and spiritual. In the first chapter, in order to show each of the mythological functions, I summarize …

Pleistocene Peccaries From Guy Wilson Cave, Sullivan County, Tennessee., April Season Nye

Electronic Theses and Dissertations

Descriptive and taphonomic analyses of undescribed Pleistocene Tayassuidae from Guy Wilson Cave within the East Tennessee State University and McClung Museum collections revealed a MNI of 16 Platygonus compressus and 2 Mylohyus from left femora and isolated teeth, respectively. Linkage between upper dentition and species identification is suggested by comparing Mylohyus fossilis to M. nasutus from other Pleistocene-aged sites. Long-bone NISP and age profiles show a predominance of Platygonus adults. Tayassuidae upper canines, likely Platygonus, suggest sexual dimorphism. Long bones were analyzed for carnivore damage and utilization revealing light utilization similar to that caused by modern wolves. Long bone …

Vitamin E (Tocotrienols) And Prostate Cancer: A Proteomics Approach., Christian Mbangha Muenyi

Electronic Theses and Dissertations

Proteomics is the large scale study of proteins in cells or organisms. The purpose of this study was to characterize the proteomic alterations occurring in a prostate cancer (LNCaP) cell line after treatment with delta-tocotrienol (a form of vitamin E not very prevalent from most dietary sources). We found that both gamma- and delta-tocotrienols induced time and concentration dependent growth inhibition and programmed cell dead (apoptosis) in LNCaP cells. Secondly, we used two-dimensional gel electrophoresis (2-DE) to characterize changes in protein expression levels associated with this treatment. Our results show that a specific set of proteins are regulated at both …

Confidence Intervals For Population Size In A Capture-Recapture Problem., Xiao Zhang

Electronic Theses and Dissertations

In a single capture-recapture problem, two new Wilson methods for interval estimation of population size are derived. Classical Chapman interval, Wilson and Wilson-cc intervals are examined and compared in terms of their expected interval width and exact coverage properties in two models. The new approach performs better than the Chapman in each model. Bayesian analysis also gives a different way to estimate population size.

The Effect Of Caffeine And Ethanol On Flatworm Regeneration., Erica Leighanne Collins

Electronic Theses and Dissertations

Flatworms, or planarian, have a high potential for regeneration and have been used as a model to investigate regeneration and stem cell biology for over a century. Chemicals, temperature, and seasonal factors can influence planarian regeneration. Caffeine and ethanol are two widely used drugs and their effect on flatworm regeneration was evaluated in this experiment. Non-toxic levels of caffeine, a stimulant, and ethanol, a depressant, were determined. The tails of the flatworms were removed and the regeneration stage was analyzed every 3 days for 15 days to see the effect of these drugs alone and in combination on regeneration. For …

Nicotine Sensitization In Β-Arrestin 2 Knockout Adolescent Mice., Jennifer A. Correll

Electronic Theses and Dissertations

ß arrestin-2 is a protein involved in signaling of D2 receptors and plays a mediating role in sensitization to psychostimulants and the opiate morphine. In this study, 3-4 week old BA-2 KO and wild type C57/B6 mice received nicotine tartarate (s.c, 0.5 mg/kg free base) for 7 or 14 consecutive days followed by a drug-free period. An acute nicotine challenge followed the drugfree period. Results indicated that the absence of ß-arrestin-2 reduced sensitization to nicotine in Experiment 1. BA-2 KOs eventually demonstrated sensitization in Experiment 2. However, absence of ß-arrestin-2 blocked expression of sensitization on the challenge. After the challenge, …

The Effects Of Movement On Literacy., Kathy S. Luppe

Electronic Theses and Dissertations

The purpose of this study was to extend the knowledge of the relationship between movement and cognition by examining the impact of a sensory-motor program on the literacy skills of first grade learners from one elementary school in East Tennessee.

Literacy skills were evaluated using five subtests of the Dynamic Indicators of Basic Early Literacy Skills (DIBELS) assessment.

A 2 x 2 x 2 mixed factorial MANOVA was used to analyze group, gender, and test time effects on multiple dependent variables. The analysis yielded a statistically significant result (p < .05) in gains for the movement group and a …

Evaluation Of An Elementary School Wellness Concept In Rural East Tennessee., Audrey Lynn Taylor

Electronic Theses and Dissertations

Parents of elementary school children in the small, rural area of Unicoi County, TN were surveyed to determine their attitudes toward health, nutrition, and Unicoi County Schools' Wellness Policy. Elementary school classrooms were randomly chosen to receive surveys for the children's parents to return by mail. Data were compiled and analyzed using SPSS software. Over 99% of parents stated that nutrition education in schools was important, and 96% stated schools played an important role in their child's health. The assessment provided meaningful data and laid groundwork for future nutrition education programs. The research showed rural, lower-income parents are supportive of …

The Effects Of Nicotine Conditioned Place Preference In D2 Primed Adolescent Rats: Age-Related And Gender Effects., Yoko Emily Ogawa

Electronic Theses and Dissertations

This study investigated nicotine conditioned place preference (CPP) in two different ages of adolescence using a rodent model of schizophrenia. Both 2- and 3-chambered CPP apparatuses were used to test whether the CPP was due to an aversion to the white chamber. Animals were neontally treated with the dopamine D₂/D₃ agonist, quinpirole, or saline and raised to either early postweanling age (P 22) or adolescence (P 29). Rats were conditioned to prefer the white chamber using nicotine. Results showed that nicotine induced CPP and appeared to alleviate an increased stress response in D₂ primed animals, which …