Digital Commons Network^™

New Microarray Image Segmentation Using Segmentation Based Contours Method, Yuan Cheng

Doctoral Dissertations

The goal of the research developed in this dissertation is to develop a more accurate segmentation method for Affymetrix microarray images. The Affymetrix microarray biotechnologies have become increasingly important in the biomedical research field. Affymetrix microarray images are widely used in disease diagnostics and disease control. They are capable of monitoring the expression levels of thousands of genes simultaneously. Hence, scientists can get a deep understanding on genomic regulation, interaction and expression by using such tools.

We also introduce a novel Affymetrix microarray image simulation model and how the Affymetrix microarray image is simulated by using this model. This simulation …

Microfluidic Devices Applied On Enriching Post –Translational Modified Proteins For Proteomics, Hui Xia

Doctoral Dissertations

In this work, microfluidic devices were developed for enriching post-translational modified proteins. Post-translational modifications (PTM) of proteins play essential roles in cellular physiology and disease. The identification of protein substrates and detection of modification site helps understand PTM-mediated regulation in essential biological pathways and functions in various diseases. However, PTM proteins are typically present only at trace levels, making them difficult to identify in mass spectrometry based proteomics. This work study is about the design, fabrication and testing of the microfluidic device for the enrichment of abundant amount of PTMs. Carbonylated protein is used as a representative PTM to illustrate …

Rheological Study Of Asphalt Binders With A Wax-Based Warm Mix Additive And Its Relationships To Mix Compaction And Rutting, Rajan Saha

Doctoral Dissertations

Although the energy saving Warm Mix Asphalt (WMA) technology that produces asphalt mixes at lower temperatures are around for about a decade, there still exist some concerns that are hindering its wide spread implementation. The overall objective of this study is to investigate if the viscosity reductions at higher temperatures have any impacts on asphalt mix compaction and rutting performances. This study reveals that there exists a critical temperature for each asphalt binder below which viscosity will increase with addition of Sasobit®. Therefore, compaction below the critical temperature can negatively impact density.

For PG 64-22, the critical temperature is 104 …

Ultrasonication Assisted Layer-By-Layer Technology For The Preparation Of Multi-Functional Anticancer Drugs Paclitaxel And Lapatinib, Xingcai Zhang

Doctoral Dissertations

In this dissertation, ultrasonication assisted Layer-by-Layer (LbL) technology for the preparation of multifunctional poorly water-soluble anticancer drug nanoparticles, paclitaxel and lapatinib, has been developed. Many FDA approved drugs are very low soluble in water; therefore, it is very difficult to load and control their release and targeting efficiently, which greatly confines their application. The development of this method will pave the way for the development and application of those low soluble anticancer drugs.

In the first part of this dissertation, the first approach for powerful ultrasonication, the top-down approach (sonicating bulk drug crystals in polyelectrolyte solution), was successfully applied for …

Using Power-Law Properties Of Social Groups For Cloud Defense And Community Detection, Justin L. Rice

Doctoral Dissertations

The power-law distribution can be used to describe various aspects of social group behavior. For mussels, sociobiological research has shown that the Lévy walk best describes their self-organizing movement strategy. A mussel's step length is drawn from a power-law distribution, and its direction is drawn from a uniform distribution. In the area of social networks, theories such as preferential attachment seek to explain why the degree distribution tends to be scale-free. The aim of this dissertation is to glean insight from these works to help solve problems in two domains: cloud computing systems and community detection.

Privacy and security are …

Engineering Microenvironments To Modulate Calcium Information Processing In Neuronal Cells, Kinsey Cotton Kelly

Doctoral Dissertations

Tissue engineered microenvironments were constructed to test the effects glial cells have on calcium information processing, and to mimic conditions in vivo for tumor invasion and residual cancer after resection of tumor. Submaximal, nM, glutamate (GLU) stimuli were applied to the engineered environments, and the resulting calcium dynamic behavior of neuronal cells was measured to help predict and interpret chaotic systems in the experimental realm. Calcium is a key signaling ion which signals through the N-methyl-D-aspartate (NMDA) glutamate receptor on the neuronal membrane. GLU binding to the NMDA receptor (NMDAR) causes a large and dynamic increase in neuronal intracellular calcium. …

Bioactive Glass Scaffolds For The Regeneration Of Load-Bearing Bone, Xin Liu

Doctoral Dissertations

"Biocompatible scaffolds that replicate the structure and function of bone would be ideal bone substitutes for structural bone loss, provided they have the requisite mechanical properties for reliable long-term loading. In this dissertation, strong porous scaffolds of silicate 13-93 bioactive glass, created with two different microstructures, were evaluated to determine their mechanical properties and their capacity to regenerate bone in a rat calvarial defect model. Scaffolds with an oriented microstructure of columnar pores were prepared by unidirectional freezing of camphene-based suspensions, followed by thermal annealing and sintering. By optimizing the freezing conditions, annealing time, and sintering temperature, constructs (porosity = …

Two Essays On Dynamic Programming And Reinforcement Learning, Shuva Ghosh

Doctoral Dissertations

"The semi-Markov decision process (SMDP) is a variant of the Markov decision process (MOP). This dissertation work focuses on the application of SMDPs to disaster response management and to maintenance management. Average and discounted reward are two popular performance metrics for MDPs/SMDPs. While both dynamic programming (DP) methods, i.e., value iteration and policy iteration, are commonly used to solve MDPs/SMDPs, value iteration is easier to apply than policy iteration. The existing value iteration algorithms for average reward SMDPs have some noteworthy limitations, which are sought to be overcome in this work. Reinforcement learning (RL) techniques, which are also studied in …

Nanoscale Metal Oxide And Supported Metal Catalysts For Li-Air Battery, Kan Huang

Doctoral Dissertations

"The dissertation work focuses on research and development of durable nanoscale catalysts and supports for rechargeable Li-air batteries that use aqueous catholytes. Transition metal oxides, TiO₂ and Nb₂O₅ in particular, were prepared from a sol-gel process in the form of nanocoatings (5~50 nm) on carbon nanotubes (CNTs) and studied as catalyst supports. Carbon doping in the oxides and post annealing significantly increased their electronic conductivity. Pt catalyst on the support with TiO₂ (Pt/c-TiO₂/CNTs) showed a much better oxygen reduction reaction (ORR) activity than a commercial Pt on carbon black (Pt/C). Negligible loss ( …

Creating New Superconducting & Semiconducting Nanomaterials And Investingating The Effect Of Reduced Dimensionality On Their Properties, Sukhada Mishra

Doctoral Dissertations

"The field of nanomaterials has continued to attract researchers to understand the fundamentals and to investigate potential applications in the fields of semiconductor physics, microfabrication, nanomedicine, surface sciences etc. One of the most critical aspects of the nanomaterials research is to establish synthetic protocols, which can address the underlying product requirements of reproducibility, homogenous morphology and controlled elemental composition. We have focused our research in exploring synthetic routes for the synthesis of superconducting and semiconducting nanomaterials and analyze their structure--property relationship through detailed characterizations. The first part of dissertation is focused on the synthesis of superconducting FeSe nanostructures using catalyst …

Fundamental Properties Of Colloidal Unimolecular Polymer Particles, Minghang Chen

Doctoral Dissertations

"Colloidal Unimolecular Polymer (CUP) particles are a new genre of material formed by self-assembly into spheroidal particles due to the effect of hydrophilic/hydrophobic interactions of the polymer with a change in the solvent. The particle sizes were characterized by means of Dynamic Light Scattering with corrected viscosities instead of water's viscosity. The distribution of particle size agreed well with the distribution of molecular weight proving that the polymer had undergone unimolecular collapse. The rheology study shows the presence of surface water and its significant effect on the rheology. The primary and secondary electroviscous effects were also found to play roles …

Efficient Uncertainty Quantification In Aerospace Analysis And Design, Yi Zhang

Doctoral Dissertations

"The main purpose of this study is to apply a computationally efficient uncertainty quantification approach, Non-Intrusive Polynomial Chaos (NIPC) based stochastic expansions, to robust aerospace analysis and design under mixed (aleatory and epistemic) uncertainties and demonstrate this technique on model problems and robust aerodynamic optimization. The proposed optimization approach utilizes stochastic response surfaces obtained with NIPC methods to approximate the objective function and the constraints in the optimization formulation. The objective function includes the stochastic measures which are minimized simultaneously to ensure the robustness of the final design to both aleatory and epistemic uncertainties. For model problems with mixed uncertainties, …

Fiber Inline Pressure And Acoustic Sensor Fabricated With Femtosecond Laser, Yinan Zhang

Doctoral Dissertations

"Pressure and acoustic measurements are required in many industrial applications such as down-hole oil well monitoring, structural heath monitoring, engine monitoring, study of aerodynamics, etc. Conventional sensors are difficult to apply due to the high temperature, electromagnetic-interference noise and limited space in such environments. Fiber optic sensors have been developed since the last century and have proved themselves good candidates in such harsh environment. This dissertation aims to design, develop and demonstrate miniaturized fiber pressure/acoustic sensors for harsh environment applications through femtosecond laser fabrication. Working towards this objective, the dissertation explored two types of fiber inline microsensors fabricated by femtosecond …

Optimized Techniques For Real-Time Microwave And Millimeter Wave Sar Imaging, Joseph T. Case

Doctoral Dissertations

"Microwave and millimeter wave synthetic aperture radar (SAR)-based imaging techniques, used for nondestructive evaluation (NDE), have shown tremendous usefulness for the inspection of a wide variety of complex composite materials and structures. Studies were performed for the optimization of uniform and nonuniform sampling (i.e., measurement positions) since existing formulations of SAR resolution and sampling criteria do not account for all of the physical characteristics of a measurement (e.g., 2D limited-size aperture, electric field decreasing with distance from the measuring antenna, etc.) and nonuniform sampling criteria supports sampling below the Nyquist rate. The results of these studies demonstrate optimum sampling given …

Processing, Microstructural Evolution And Electrochemical Performance Relationships In Solid Oxide Fuel Cells, Ayhan Sarikaya

Doctoral Dissertations

"The relationships between the processing parameters, microstructures and electrochemical performance of solid oxide fuel cell (SOFC) components were investigated. The operating regimes (i.e., reducing vs. oxidizing) as well as the elevated temperatures (e.g. 800°C) for their operation introduce several material challenges. Therefore, composite materials are employed to withstand operating conditions while providing sufficient electrochemical performance for fuel cell operation. Analyses on lanthanum-strontium manganite (LSM) - yttria stabilized zirconia (YSZ) compositions (45 vol%-55 vol%) by impedance spectroscopy demonstrated that two competing polarization mechanisms (i.e. charge-exchange and surface adsorption-diffusion of oxygen) limit performance. Optimization of microstructures resulted in total resistances as low …

Risk Analysis: Comparative Study Of Various Techniques, Hanan Mohammad Altabbakh

Doctoral Dissertations

"Researchers in the safety field are facing more challenges everyday with the expanding modern socio-technical systems. Safety analysis such as hazard analysis, accident causation analysis, and risk assessment are being revisited to overcome the shortcoming of the conventional safety analysis. With increasingly complex human system interaction in today's modern systems, new safety challenges are being faced that needed to be assessed and addressed. Managers and engineers face the challenge to choose from the vast amount of techniques available and utilize the correct one. Indeed, new or improved risk assessment tools that can address these complexities are needed. One of the …

Scaleup And Hydrodynamics Study Of Gas-Solid Spouted Beds, Shreekanta Basavaraj Aradhya

Doctoral Dissertations

"A thorough understanding of the complex flow structure of gas-solid spouted bed is crucial for design, scale-up and performance. Advanced gas-solid optical probes were developed and used to evaluate different hydrodynamic parameters of spouted beds. These optical probes measure solids concentration, velocity and their time series fluctuations. Since solids concentration needs to be converted to solids holdup through calibration, for meaningful interpretation of results, a novel calibration method was proposed (which is inexpensive and reliable compared to the current reported methods) and validated in the present study. The reported dimensionless groups approach of spouted bed scale-up was assessed and was …

Social-Context Based Routing And Security In Delay Tolerant Networks, Roy A. Cabaniss

Doctoral Dissertations

"Delay Tolerant Networks (DTNs) were originally intended for interplanetary communications and have been applied to a series of difficult environments: wireless sensor networks, unmanned aerial vehicles, and short-range personal communications. There is a class of such environments in which nodes follow semi-predictable social patterns, such as wildlife tracking or personal devices. This work introduces a series of algorithms designed to identify the social patterns present in these environments and apply this data to difficult problems, such as efficient message routing and content distribution. Security is also difficult in a mobile environment. This is especially the case in the event that …

Shear And Fracture Behavior Of High Performance Concretes, Mahdi Arezoumandi

Doctoral Dissertations

"Concrete is the most widely used man-made material in the world. Like other materials, it has been improved over time. Nowadays, with continued development of science and technology, a new generation of concretes is produced and is termed high performance concretes. A review of previously published work indicates that very few studies (in some cases none) have addressed the structural behavior of full-scale, high performance concrete elements. This study investigated the shear and fracture behavior of two types of high performance concrete - high-volume fly ash concrete (HVFAC) and self-consolidating concrete (SCC). The HVFAC incorporates up to 70% cement replacement …

Structural And Magnetic Properties Of Ni And Cr Substituted La₀.₇Sr₀.₃Mno₃, Thomas Franklin Creel Jr.

Doctoral Dissertations

"The La₁₋ₓSrₓMn_1-yB_yO₃ perovskite systems where B represents a 3d transition metal such as Cr, Fe, Co, Ni, Cu, and Zn, are of significant interest due to their large magnetoresistance, and electronic transport properties that change significantly, depending on values of x and which transition metal used. Their applications in technology include cathodes for solid oxide fuel cells, resistance random access memory and catalysis. At certain doping concentrations on the B-site, metal-to-semiconductor or metal-to-insulator transitions coupled with colossal magnetoresistance occur, decreasing the resistivity (up to six orders of magnitude) upon the application of an external magnetic field. …

Effect Of Dense Heat Exchanging Internals On The Hydrodynamics Of Bubble Column Reactors Using Non-Invasive Measurement Techniques, Mohammed Khloofh Al Mesfer

Doctoral Dissertations

"Given their efficiency and capital cost reduction, bubble/slurry bubble column reactors are the reactors of choice for Fischer-Tropsch (FT) synthesis, offering clean alternative fuels and chemicals. FT synthesis is an exothermic process that requires many heat exchanging tubes in order to remove heat efficiently and maintain the desired temperature and isothermal operating condition. The impact of the heat exchanging tubes (internals) on the hydrodynamics is not fully understood. Reliably designing and scaling up bubble column reactors requires proper understanding of hydrodynamics, as well as heat and mass transfer parameters.

The main objective of this work is to advance the understanding …

Minimizing Formation Damage For Preformed Particle Gels Treatment In Mature Reservoirs, Mahmoud Omran Elsharafi

Doctoral Dissertations

"This research studied the effect of preformed particle gels (PPGs) on unswept, low-permeable zones/areas. The objective was to find methods that minimized the damage caused by PPGs on unswept, low-permeable zones/areas, thus improving PPG treatment efficiency. These results can help to identify of the best PPG types, particle sizes, and brine concentrations for specific reservoirs and treatments. This study used sandstone core samples with various degrees of permeability. Two types of particle gels, a strong Daqing gel (DQ) and a weak Liquiblock™ 40k gel (40K), were used, each with a different strength.

This research included two stages. Firstly, both a …

Source Reconstructions For Ic Radiated Emissions Based On Magnitude-Only Near-Field Scanning, Ji Zhang

Doctoral Dissertations

"In this dissertation, a source reconstruction method is proposed to model the emission behavior of an IC by using magnitude-only information during near-field scanning. The phase-retrieval is accomplished during the back-forth iterations between two scanning planes of different heights. The magnitudes of fields on two planes of different heights are recorded and used to constrain the amplitude of the calculated fields during iterations. Three essential transformations among dipoles and fields are continued at each iteration step, until the convergence condition is achieved. The proposed algorithm has been verified by using a set of dipole-array as the original emitting sources, and …

Topics In Voice Echo Cancellation, Pratik Vijay Shah

Doctoral Dissertations

"Acoustic echo cancellation (AEC) is a well studied problem. The underlying assumption in most echo cancellation solutions is that the echo path following the reference signal is completely linear. However, in many handheld devices, the echo path following the reference signal is nonlinear. The reason for this nonlinearity in the echo path is the use of smaller and cheaper loudspeakers. In order to cut the manufacturing cost, device manufactures use cheaper loudspeakers such that they satisfy the carrier specifications. Such loudspeaker can be easily over driven in to their nonlinear region and thus add nonlinearities to the downlink path. This …

Advances In Blind Source Separation, Christopher Osterwise

Doctoral Dissertations

"Blind Source Separation (BSS) is a classic problem that attempts to separate unknown sources from observed mixtures. A common framework of this is the Cocktail Party problem, where multiple individuals speaking in a noisy room are recorded by a configuration of microphones, with the challenge being to split the observed signals into individual conversations. This dissertation covers the evaluation of an existing algorithm known as Cascaded ICA with Intervention Alignment (CICAIA), the establishment of a new parameter for characterizing blind mixtures, and three new algorithms, of which two are capable of separating any number of sources given more microphones than …

Behavior And Effects Of Nanoparticles In Complex Fluid Systems, Chen Wang

Doctoral Dissertations

"The development of nanoscale technologies has greatly expanded the scientific and engineering horizons and also has the potential to turn molecular discoveries into worldwide benefits on multiple fronts. Despite recent extensive efforts, the focus of nanoparticle studies has been on their synthesis and various possible derivatives. In this dissertation, molecular based modeling and simulation studies have been performed to characterize and understand the behavior, properties, and effects of nanoparticles in some important material systems. The results show that small nanoparticles that are highly faceted have significant shape effects, which are not captured by classical continuum-based approaches. When dissolved in confined …

Modeling And Analysis Of Dynamic Behavior Of Adsorption Stratified Column Beds Packed With Partially Fractal Porous Adsorbent Particles, Min Li

Doctoral Dissertations

"In this work novel porous adsorbent media having partially fractal structures and stratified packed-beds are constructed and their dynamic performance with respect to their adsorptive capacity through the construction and solution of rigorous models is studied. The results show that conventional columns packed with partially fractal porous adsorbent particles provide larger breakthrough times and dynamic utilization of the adsorptive capacity of the column when compared with those of conventional columns employing conventional adsorbent particles which do not possess a partially fractal structure. Furthermore, stratified column beds exhibit superior species separation performance than conventional columns when they are packed with either …

Spatial Variation Of Amd Related Co₂ Emissions On Reclaimed Mine Spoil, Moagabo Mathiba

Doctoral Dissertations

"Carbon dioxide (CO₂) from acid mine drainage (AMD)-carbonate neutralization reactions in reclaimed mine spoils, can intermittently invade homes and structures constructed on or adjacent to these spoils in hazardous and even lethal concentrations (>15%) and rendering them uninhabitable. This phenomenon is beginning to be recognized as an emerging environmental, safety and health threat with potential environmental and financial liability for mine operators, developers and owners.

Like in natural soils, soil temperature and moisture, and possibly elevation are likely to influence CO₂ emissions from reclaimed mine spoils. However, mine spoil soils are heterogeneous and have significant convective transport due to …

Bio-Inspired Design, Fabrication And Testing Of Bipolar Plates For Pem Fuel Cells, Nannan Guo

Doctoral Dissertations

"The flow field of a bipolar plate distributes reactants for polymer electrolyte membrane (PEM) fuel cells and removes the produced water from the fuel cells. It greatly influences the performance of fuel cells, especially the concentration losses. Two approaches were developed to improve flow field designs in this dissertation. One is inspired by the biological circulatory structures and called bio-inspired designs, which have great potential to transport reactant efficiently and hence improve fuel cell performance. Another way is using a network-based optimization model to optimize the conventional flow field configurations, i.e., pin-type, parallel and serpentine designs, to improve flow distributions …

Inverter Design And Analysis Using Multiple Reference Frame Theory, Luke Dale Watson

Doctoral Dissertations

"Multiple reference frame theory allows for periodically time varying signals to be represented as a set of dc signals. In other words, every periodic signal can be expanded into a Fourier series representation. By modeling an inverter connected to a boost maximum power point tracker (MPPT) in this manner, frequency transfer properties can be preserved and harmonics throughout the system can be predicted. A state space model taking into account the dc and fundamental grid frequency is presented and used to optimize the controller gains of the system. Using information from the dq-axis values of the measured grid current and …