Digital Commons Network^™

Community-Based Early Dementia Advance Care Planning In The United States, Christine Noelle Flaherty

Dissertations

Dementia Advance Care Planning (ACP) promotes quality of life through discussions and decisions about goals of care and end-of-life wishes. However, ACP is rarely introduced in the community setting, and ACP is commonly deferred until advanced dementia when the patient can no longer communicate. The purpose of this three-manuscript dissertation was to advance the understanding of community-based early dementia ACP through an examination of the literature (manuscript one), the development of the dementia public health exposome conceptual framework (manuscript two), and the exploration of perceptions of ACP amongst dementia clinicians, dementia caregivers, and individuals with preclinical dementia in a dementia …

Improving Undergraduate Student Engagement In Stem Through Bioinformatics Research, Michele C. Morris

Dissertations

A novel intervention to introduce undergraduate students to bioinformatics, provide authentic research opportunities, and support student retention in science majors and careers is presented in the Characterizing Our DNA Exceptions (CODE) project. The need to increase the number of qualified STEM graduates, particularly in the fields of bioinformatics and computational research, was the impetus for this project that leads students down the path of scientific discovery as they characterize genomic variants of uncertain significance (VUS). This study sought to examine if student participation in a CODE research project would increase their bioinformatics awareness, interest, comfort, and knowledge, as well as …

Novel Approaches To The Analysis Of Gene Regulation Data, Brian S. Roberts

Dissertations

The study of gene regulation is crucial to understanding differentiation, cell specialization, and response to external stimuli observed in multicellular organisms. Genome-scale technologies enabled by high-throughput sequencing have allowed insight into many aspects of gene regulation. However, these techniques produce large, complex data that require the development of computation-based analytical techniques. I present here two analytical techniques that I have created for gene regulation data. First, I describe an approach for the analysis of sequencing-based reporter data that reveals new features of human genetic sequence that generate biased strand transcription even out of genomic context. Next, I present a package …

Modeling Freely Flying Insects Using A Strongly Coupled High Fidelity Numerical Framework, Jeremy A. Pohly

Dissertations

Flying insects, with their remarkable abilities, serve as fascinating sources of inspiration. Deciphering the complex mechanisms that enable monarch butterflies to migrate nearly 80 million times their body length, requires advanced modeling tools. These tools must capture the interplay between non-linear wing aerodynamics, dynamic body responses, and flexible wings, characteristics of the monarch. A novel framework is introduced that tightly integrates these physical phenomena through a dynamic relaxation scheme. The model’s application extends to examining the longitudinal flight of monarch butterflies and the hovering flight of fruit flies, bumblebees, and hawkmoths. A bio-inspired feedback controller ensures stable flight across all …

Predictors Of Bullying Behaviors Among Adolescents In Saudi Arabia: The Role Of Self-Esteem, Emotional Intelligence And Moral Disengagement, Ayat Hamzah

Dissertations

Problem

Bullying issues are increasing among school-age children worldwide. Children and adolescents involved in bullying as victims, perpetrators, or both are likely to experience negative consequences on their emotional, social, and academic levels (Espelage & Holt, 2001). In spite of the increasing research on bullying in Saudi Arabia, there exists gaps in the literature, especially in the role of individual traits (self-esteem, emotional intelligence) and cognitive processes (moral disengagement) on bullying behavior. Additionally, using modeling analysis to understand the predictive factors affecting bullying involvement processes also filled in some of the gaps in the literature. The current study investigated the …

Mammalian Dihydropyrimidine Dehydrogenase: Employing Transient State Kinetic Methods To Determine Catalytic And Inhibitory Mechanisms, Dariush Forouzesh

Dissertations

Dihydropyrimidine dehydrogenase (DPD) catalyzes the two-electron reduction of pyrimidine bases uracil and thymine as the first step in pyrimidine catabolism. The enzyme achieves this simple chemistry using a complex cofactor set including two flavins and four Fe4S4 centers. The flavins, FAD and FMN, interact with respective NADPH and pyrimidine substrates and the iron-sulfur centers form an electron transfer wire that links the two active sites that are separated by 56 Å. DPD accepts the common antineoplastic agent 5-fluorouracil (5FU) as a substrate and so undermines the establishment of efficacious toxicity. Though studied for multiple decades, a precise description of the …

Method Development For The Quantification Of Heavy Metals In Biological Matrices And Its Application To Clinical And Environmental Samples, Michelle Catherine Gende

Dissertations

A three-fold approach was taken to develop a method for the detection of heavy metals in different matrices and had to be performed sequentially. The first was to develop a method capable of quantifying lead in complex matrices by graphite furnace atomic absorption (GFAA). The developed method needed to meet specific analytical requirements, namely, to be robust, sensitive, and accurate. Initially, four different sample preparation methods were explored for the quantification of lead using Escherichia coli (E. coli) as a sample matrix. The sample preparation procedures attempted were acidic dilution, matrix modification, conventional heating digestion, and microwave assisted acid digestion …

Role Of Micrornas In Intestinal Inflammation And Barrier Homeostasis After Alcohol And Burn Injury, Caroline J. Herrnreiter

Dissertations

MicroRNAs are small noncoding RNA molecules that negatively regulate gene expression. Within the intestinal epithelium, miRNAs play a critical role in gut homeostasis and aberrant miRNA expression has been implicated in various disorders associated with intestinal inflammation and barrier disruption. In this study, we sought to profile changes in intestinal epithelial cell miRNA expression after alcohol and burn injury and elucidate their impact on inflammation and barrier integrity. In a more targeted approach, we began by focusing on anti-inflammatory miRNAs that, when downregulated, could exacerbate inflammation and result in intestinal barrier disruption. Using a mouse model of acute ethanol intoxication …

Joint Sonar And Communication (Soncomm) System Using Solvable Chaos, Tamseel Mahmood Syed

Dissertations

An ever increasing number of actively interconnected wireless devices tend to exert congestion on the naturally limited resources provided by the electromagnetic spectrum. This fact imposes a significant engineering challenge on efficient utilization of communication channels. One method to lessen the stringency of the spectrum access is to combine functionality of wireless applications via jointly operational (multi-purpose) waveforms. Solvable chaos was previously shown to have inherent detection benefits when used independently in practical demonstrations of either acoustic ranging or communication. This dissertation focuses on the design of a joint sonar-communication (SonComm) system via solvable chaotic waveforms. First, individual hardware demonstrations …

Bioimpedance-Based Real-Time Wearable Physiological Monitoring, Robert Joseph Mathews

Dissertations

Cardiovascular and respiratory diseases account for nearly forty percent of worldwide deaths. A critical factor in preventing these deaths is early detection, treatment, and monitoring of the diseases. Remote patient monitoring coincides with significant advances in integrated circuit technology resulting in greater performance and lower power consumption. In this work, novel monitoring configurations and frequency excitations are assessed across ten subjects to evaluate their suitability for monitoring cardiac and respiratory activity. A custom bioimpedance controller board was fabricated and integrated into the existing UAH euHy board. In the pilot experiment, the average inter-beat interval (IBI) derived from bioimpedance compared with …

Self-Powered Sensor Systems For Space Applications, Saininad Chandrakant Naik

Dissertations

Harvesting energy from ambient sources allows sensors and systems to become self-reliable and not depend on small batteries or energy sources, which is of paramount importance in space applications. In recent decades, multi-band or wideband energy harvesters have attracted much attention. The main challenge to design such energy harvesters is that they need to efficiently scavenge the electromagnetic waves at multiple bands and deliver the received multi-frequency signals to the rectifier that has a frequency dependent impedance. In this dissertation, a compact dual-band harvesting sensor is developed for space applications at the RF (900 MHz) and the ISM (2400 MHz) …

An Assessment Of Actual Evapotranspiration And Soil Moisture Functions Based On Local-Scale Data In Alabama, Lisa Lugo Kuzy

Dissertations

Large-scale models and remote technology are widely used for the estimation of the energy budget at a global scale. The interaction of energy fluxes and soil water at the vegetation root zone is the driving force that controls the evapotranspiration process. Although the importance of soil moisture, it can only be measured at a point scale, given the great heterogeneity of soil profile. Large-scale models struggle to capture the spatial heterogeneity of soils, and remote sensing can only observe the surface level soil moisture, omitting the root zone. Studies have shown evapotranspiration estimates (specifically from Remote Sensing) and can provide …

Speleogenomics Of Cave Life In North America, Joseph B. Benito

Dissertations

Cave organisms with their unusual morphologies including loss of eyes, lack of body pigmentation, and the compensatory improvement of nonvisual sensory systems have long intrigued and fascinated biologists. Due to their restricted distributions and life history traits, many troglomorphic (subterranean-adapted) species are considered imperiled and are high priority targets for protective management. With increasing anthropogenic threats to cave ecosystems, it is important to study cave-dwelling organisms, so that informed management decisions can be made on a regular basis. Detailed information on the genetics, taxonomy, distribution, and colonization history of cavernicoles are necessary to make rigorous ecological inference and develop respective …

Molecular Interactions In Engineering: Peptide-Driven T Cell Activation And Accelerated Drug Discovery Applications, Armin Ahmadi

Dissertations

In pursuit of an interdisciplinary exploration of molecular interactions from an engineering perspective with a focus on protein-protein and protein-small molecule interactions, multiple research projects were undertaken, leveraging both experimental and computational approaches. The focus of the wet-lab experimental project was on the identification and evaluation of a novel peptide ligand that binds specifically to the human CD3ε and study how this interaction influences T-Cell activation, proliferation, and calcium signaling. A novel peptide ligand, WSLGYTG, was discovered through phage display peptide technology. Peptide interaction to the CD3ε receptor was simulated using molecular docking calculations. This peptide ligand was synthesized and …

“Can I Get Some Help, Please?”: Examining Contributing Factors To First-Generation Student Success In California Community Colleges, Tijan G. White

Dissertations

This study aimed to determine the percentage of first-generation college students enrolled at California Community Colleges and examine their experiences through a culturally engaging lens using a mixed-methods approach. The research was interested in determining what percentage of community college students hold first-generation college student status using the federal definition of the term, understanding to what extent those students found their campus environments to be culturally responsive and relevant to their needs and what insights some of these students could offer regarding their experiences. The researcher surveyed and facilitated a focus group discussion with students throughout the Bay Area geographic …

Voice Of The Inaudible: Well-Being Of Chinese American Young Adults, Chinese American Parents’ Parenting Competency, And The Correlation Of The Two, Diana Huang

Dissertations

Violence against Asian Americans since the beginning of the COVID-19 pandemic has become a national crisis in the United States. Racism against Asian Americans in the country, however, has been deep-rooted since the nineteenth century. Chinese American adolescents, who are susceptible to identity crisis as young adults and confront racial discrimination as a minority race, face distressing challenges. It is critical to inquire into their lived narratives and hear their voices. The author investigated the early life experiences of nine Chinese Americans and identified how racial identity is shaped at a young age with negative external inputs, as well as …

Elucidating The Role Of A Staphylococcal Glucosaminidase In Host Innate Immune Responses, Zachary Resko

Dissertations

The peptidoglycan of Staphylococcus aureus is a critical cell envelope constituent and virulence factor that subverts host immune defenses and provides protection against environmental stressors. Peptidoglycan chains of the S. aureus cell wall are processed to characteristically short lengths by the glucosaminidase, SagB. It is well established that peptidoglycan is an important pathogen-associated molecular pattern (PAMP) that is recognized by the host innate immune system and promotes production of pro-inflammatory cytokines, including IL-1β. However, how bacterial processing of peptidoglycan drives IL-1β production is comparatively unexplored. Here, we tested the involvement of staphylococcal glucosaminidases in shaping innate immune responses and identified …

A Social Ontological Account Of Alienation And Its Place In The History Of Alienation Theory, Philip William Bauchan

Dissertations

Alienation is a sociological term that has found itself severely out of favor as an analytical concept due to what are perceived as inextricable theoretical shortcomings despite having once enjoyed a time when it was taken to be essential for a robust and critical analysis of society. This dissertation looks to contribute to a revitalization of alienation theory by offering an understanding of alienation that is grounded in the framework of social ontology as forwarded in the works of John Searle. This social ontological account conceives of alienation as a fallout fact that arises when there is a performative contradiction …

Dynamic Control Of The Cardiac Calcium Pump, Sean Robert Cleary

Dissertations

The sarco/endoplasmic reticulum Ca2+-ATPase (SERCA) sequesters Ca2+ into the endoplasmic reticulum of cells to establish a reservoir for Ca2+ signaling. In the heart, the activity of this transporter is tightly controlled via direct interactions with two competing regulatory micropeptides: phospholamban (PLB) and dwarf open reading frame (DWORF). PLB inhibits SERCA, while DWORF activates SERCA. These competing interactions determine cardiac performance by modulating the Ca2+ signals that drive the contraction/relaxation cycle. Previous studies indicated these SERCA-micropeptide interactions are Ca2+-sensitive; SERCA binds PLB more avidly at low cytoplasmic [Ca2+] but binds DWORF better when [Ca2+] is high. Here, FRET-microscopy demonstrated that this …

Key Stakeholders' Perceptions Of Stem Education In Zambia: A Mixed Methods Inquiry, Choobe M. Maambo

Dissertations

In the recent past, the concept of STEM education has become a “buzz phrase” in the Zambian education system, and yet, very little is known about STEM education let alone what those involved in STEM think or know about STEM in the country. The primary purpose of this study was to investigate key STEM stakeholders’ perceptions of STEM education in Zambia. To better understand STEM in the country, it was critical to explore the perceptions of STEM held by STEM teachers, STEM administrators, and STEM policymakers in the perception domains of STEM vision, STEM definition, STEM purpose, STEM relevance, and …

None Of Us Are Safe: How Leaders Sustain Culturally Responsive Elementary And Middle School Improvement Planning, Jerry B. Michel

Dissertations

In the spring of 2020, school leaders, school boards, and teachers faced challenges beyond the COVID-19 pandemic in the United States, finding themselves under attack for the perceived applications of critical race theory in school curriculum. These conditions made active engagement in equity-focused school improvement planning more challenging. School leaders and leadership teams may benefit from using an established framework to identify strengths and areas for growth around culturally responsive pedagogy for effective school improvement planning. This article explores the use of Hammond’s (2015) Ready for Rigor Framework as a tool for identifying prioritizing which conditions for culturally responsive pedagogy …

Ethnic-Racial Socialization Practices Of Dominican Mothers With Their Young Children: Understanding Practices With A Sociohistorical Context Framework, Sabrina Altagracia Mendez-Escobar

Dissertations

Parents who self-identify as members of a community of color engage in practices aimed at teaching their children what it means to be a member of their ethnic-racial group which are called ethnic-racial socialization (ERS) practices. Promoting a positive sense of ethnic-racial identity through ERS contributes to balancing the negative effects of discrimination which can be helpful to children who are part of a marginalized group in the US including Latinx children. However, when the family practices of Latinx families are explored in the literature, various Latinx groups are lumped together. When studying ERS, research should aim to consider Latinx …

Establishing A Health-Based Social Hierarchy, Akila Salaam Raoul

Dissertations

Social dominance theory (SDT) proposes a basic human nature to form social hierarchies based on social group membership with dominant and subordinate social groups. These social hierarchies are maintained through legitimizing myths, or beliefs that work to frame the existing social system as fair by suggesting that individuals receive the social status they deserve based on their actions and contributions to society as opposed to their group membership. The level at which an individual wants to maintain social hierarchies is indicated by their Social Dominance Orientation (SDO). SDT has been extensively studied in the context of race, gender, and age …

Characterization Of Mroq-Dependent Regulation Of Staphylococcus Aureus Quorum Sensing, Madison Ruth Stock

Dissertations

Gram-positive bacteria produce small autoinducing peptides (AIPs), which act to regulate expression of genes that promote adaptive traits including virulence. The Gram-positive pathogen Staphylococcus aureus generates a cyclic AIP that controls expression of virulence factors via the accessory gene regulatory (Agr) system. S. aureus strains belong to one of four Agr groups (I, II, III, and IV), and each group harbors allelic variants of AgrD, the precursor of AIP. In a prior screen for S. aureus virulence factors, the Alonzo lab identified MroQ, a putative peptidase. A ΔmroQ mutant closely resembled a Δagr mutant and had significant defects in AIP …

A Comparison Of Virtual Vs. Traditional Cycling Exercise On Perceived Duration And Exercise Enjoyment, Kyle W. Reason

Dissertations

We compared perceived duration, ratings of enjoyment and RPE between virtual vs. standard cycling. Participants completed a VO2 peak test and four counterbalanced trials (two 20-min, two 40-min) one at each duration on the interactive Expresso bike (EXP) and a workload matched trial on a Velotron (VEL). Acute RPE were assessed every 5-min. Perceived duration (min) and exercise enjoyment were assessed 15-min post. No significant differences were found for perceived duration between trials (SDE 23.9 ± 8.4; SDV 27.1 ± 9.6; LDE 40.0 ± 12.1; LDV 38.9 ± 10.8). For long duration expresso (LDE) RPE-overall were significantly lower at 25, …

Structural Impact Of Epstein-Barr Virus- Encoded Small Rna -1 On Its Transport And Function, Zubaida Hassan

Dissertations

Epstein-Barr Virus (EBV)-Encoded RNAs (EBERs) are two small, noncoding, structurally conserved transcripts that are expressed in millions of copies in all stages of EBV infection. Despite their abundant expression, the role of EBERs in EBV biology or pathogenesis is not well defined. However, they are associated with driving cell proliferation and improving intercellular communication via their secretion in exosomes. In spite of the pathogenic potentials of EBERs, the mechanisms involved in their transport and function remain to be elucidated. The aim of this PhD dissertation was to investigate the structural impact of EBER1 in its transport and function. The conserved …

Photonic Monitoring Of Atmospheric Fauna, Adrien P. Genoud

Dissertations

Insects play a quintessential role in the Earth’s ecosystems and their recent decline in abundance and diversity is alarming. Monitoring their population is paramount to understand the causes of their decline, as well as to guide and evaluate the efficiency of conservation policies. Monitoring populations of flying insects is generally done using physical traps, but this method requires long and expensive laboratory analysis where each insect must be identified by qualified personnel. Lack of reliable data on insect populations is now considered a significant issue in the field of entomology, often referred to as a “data crisis” in the field. …

Twitter Sentiment Analysis: Applications In Healthcare And Finance, Jiali Wang

Dissertations

This research explores the influence of Twitter sentiment on healthcare and finance industries. It assesses how Twitter sentiment and culture measure influence COVID-19 statistics, and it investigates the impact of Twitter sentiment on S&P 1500 stock mispricing. Furthermore, it examines how tweet sentiment predicts major industry returns.

The first part examines how Hofstede’s Culture Dimensions (HCD) and Twitter economic uncertainty index (TEU) relate to COVID-19 infection rate and death rate. The results show certain aspects in HCD, such as power distance index (PDI) and masculinity (MAS) both are negatively and significantly associated with the infection rate, while indulgence (IVR) and …

Integrated Machine Learning And Optimization Approaches, Dogacan Yilmaz

Dissertations

This dissertation focuses on the integration of machine learning and optimization. Specifically, novel machine learning-based frameworks are proposed to help solve a broad range of well-known operations research problems to reduce the solution times. The first study presents a bidirectional Long Short-Term Memory framework to learn optimal solutions to sequential decision-making problems. Computational results show that the framework significantly reduces the solution time of benchmark capacitated lot-sizing problems without much loss in feasibility and optimality. Also, models trained using shorter planning horizons can successfully predict the optimal solution of the instances with longer planning horizons. For the hardest data set, …

Faceted Nanomaterial Synthesis, Characterizations And Applications In Reactive Electrochemical Membrane Filtration, Qingquan Ma

Dissertations

Facet engineering of nanomaterials, especially metals and metal oxides has become an important strategy for tuning catalytic properties and functions from heterogeneous catalysis to electrochemical catalysis, photocatalysis, biomedicine, fuel cells, and gas sensors. The catalytic properties are highly related to the surface electronic structures, surface electron transport characteristics, and active center structures of catalysts, which can be tailored by surface facet control. The aim of this doctoral dissertation research is to study the facet-dependent properties of metal or metal oxide nanoparticles using multiple advanced characterization techniques. Specifically, the novel atomic force microscope-scanning electrochemical microscope (AFM-SECM) and density functional theory (DFT) …