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Determination Of The Roles Of Non-Conserved Residues Surrounding The Functional Ggq Motif Of Rf2 In Translation Arrest, Jerusha Salome Emmanuel

Dissertations

The metabolism of bacteria is finely tuned through differential regulation of gene expression, which allows bacteria to adapt to changes in the external environment. Oftentimes, expression of genes is regulated during protein synthesis. Changes in concentration and chemical composition of factors involved in protein synthesis contribute to the regulation of the expression of genes. Such differences are often exploited by researchers to control cell growth of microorganism of health and industrially relevant. Regulation of protein synthesis often produces the machinery called ribosome to stall (also known as ribosome arrest), where interactions between ribosomal nucleotides, translation factors, the nascent peptide and …

"Let Us Make 'Adam": An Edenic Model Of Personal Ontology, Marla A. Samaan Nedelcu

Dissertations

Personal ontology studies human constitution and human nature, an increasingly debated topic in Christian theology. Historically, the most prominent models of personal ontology in Christian theology have been substance dualist models. More recently, physicalist models have offered prominent alternatives.

This dissertation studies the conflict of interpretations between these two major model groupings. By applying a canonical theology, it then presents an Edenic model of personal ontology that can address the current conflict of interpretations. To achieve this end, the dissertation briefly analyzes substance dualism and physicalism according to the rubrics of constitution and nature, using a model methodology. It then …

Retrieving And Reimagining Sanctuary And Solidarity: Racial Disparities In Infant Mortality, Alyson Capp

Dissertations

In Milwaukee, Black babies die before their first birthday nearly three times as often as White and Hispanic babies. Prematurity is the major cause of infant mortality, and social determinants of health play a large role. Commitments from within Christian bioethical traditions can critique ethical frameworks commonly in use in US bioethics by calling for the incorporation of analysis of social power dynamics that is necessary for addressing this issue. Original ethnographic fieldwork that listens closely to Black mothers and health professionals uncovers key themes related to women's and infant health at the intersection of race, class, and gender. By …

Structure-Guided T Cell Receptor Mutations That Alter Antigen Specificity, Cross-Reactivity, And Polyfunctional Phenotypes In Gene-Modified T Cells, Kendra Foley

Dissertations

Adoptive cell transfer of T cell receptor (TCR) gene-modified T cells targeting specific tumor antigens is currently in clinical trials for patients with advanced malignancies. Despite the clinical responses, there are still hurdles to be overcome in achieving an effective and safe therapy. One of the limitations in the success of this type of therapy is the potential for cross-reactivity and unanticipated off-target reactivity which could lead to autoimmunity. Adverse events encompassing these "off-target, off-tumor" cross-reactivities leading to autoimmunity have been seen in patients in different clinical trials. Here, we demonstrate a novel approach to improve antigen specific reactivity and …

Membrane Microdomains As Platforms For Extra-Cellular Fusions, Michael Hantak

Dissertations

Life requires biological membranes. Membrane-enclosed compartments separate and unite through dynamic fission and fusion reactions. These are catalyzed processes that are central in organismal biogenesis. This dissertation focuses on extracellular membrane fusions, which are central to several processes. (1) Enveloped viruses enter cells through membrane fusions. (2) Extracellular vesicles (EVs) also deliver molecules into cells through membrane fusions. (3) Entire cells also fuse together, generating fertilized zygotes, skeletal muscles, and giant cell macrophages.Mechanisms of extracellular membrane fusion are poorly understood. This dissertation aimed to further define these mechanisms. We focused on regulatory cofactors, including tetraspanins, transmembrane proteins that cluster into …

Determination Of Transforming Growth Factor-Β Signaling Targets In Activated T Cells, Ramiah Jacks

Dissertations

T lymphocytes are a critical component of the adaptive immune system and their responses in pathogenic and steady state conditions require strict regulation. One mechanism involved in this regulation is Transforming Growth Factor-β (TGF-β) signaling. TGF-β can act on activated T cells to facilitate the differentiation of TGF-β dependent T helper subsets or the suppression of T cell activation and proliferation. However, the molecular mechanism(s) by which TGF-β signaling controls T cell differentiation vs T cell suppression remain poorly understood.TGF-β signaling is propagated by initially binding to the TGF-β type II receptor, which subsequently activates TGF-β type I receptor (TGF-βRI) …

The Social And Contextual Mechanisms Of Children's Learning In Museums, Lindsay R. Maldonado

Dissertations

This study explored the associations between immersive exhibit design, mother-child engagement, conversational interactions, and children's learning. Participants were 41 mothers and their 6-8-year-old children (Mage=7.15, SD=.79). A within-subjects, mixed methods approach was utilized, including naturalistic observations, interview data, and surveys. Mother-child dyads were video and audio-recorded as they visited four different exhibits: two low immersion exhibits and two high immersion exhibits. Interview data was collected from children immediately following the visit to assess learning. Survey data was collected from mothers to assess education, environmental predispositions, and science-related career. In high immersion exhibits, mothers and children spent more time and asked …

Characterization Of The Effects Of Sex And Estrogen Receptor Signaling On Antigen-Specific T Cells For Immunotherapy, Flor Cecilia Navarro Negredo

Dissertations

Adoptive cell transfer (ACT) immunotherapy using antigen (Ag)-specific T cells is partially effective treating several malignancies but numerous challenges remain in order to improve its therapeutic potential. The roles of host factors, such as sex hormone receptor signaling, that can affect the T cell anti-tumor function remain understudied. The work performed in this dissertation characterized the role of estrogen signaling on T cell function in vitro, and during ACT immunotherapy against hepatocellular carcinoma (HCC) in vivo. Estrogen signaling through ERa enhanced the expression and secretion of Type I effector cytokines including IFNg, TNFa, and Granzyme B in male and female …

Protection From Staphylococcus Aureus Bloodstream Infection By Probiotic Exopolysaccharide, Wonbeom Paik

Dissertations

Staphylococcus aureus is known to cause severe systemic infection with high mortality rates. Antibiotics are the only approved therapy for patients, but the widespread prevalence of antibiotic resistant strains limits treatment efficacy, and many patients succumb to the disease. Many probiotic agents are commercially available, but the mechanisms by which they benefit are not known. Understanding these mechanisms will help develop novel therapeutics that can improve healthcare, including systemic infections by S. aureus. Bacillus subtilis is a probiotic bacterium that produces an exopolysaccharide (EPS) that induces anti inflammatory macrophages (MΦ). We tested if EPS could be used for systemic S. …

Behavioral Symptom Clusters, Inflammation, And Quality Of Life In Chronic Low Back Pain Patients, Anitha Saravanan

Dissertations

Chronic low back pain (CLBP) is a prevalent condition, often involving an inflammatory process. Those with CLBP frequently experience behavioral symptoms, including depressed mood, fatigue, and sleep disturbance, which may exacerbate pain and reduce quality of life (QOL). The purpose of this study was to identify behavioral symptom clusters (depressive mood, fatigue, poor sleep) in individuals with CLBP, and to determine whether there are differences in pain, QOL and inflammation (plasma IL-6) based on cluster membership. CLBP patients (N=69; age = 56±13 years) completed measures of pain, depressive mood, fatigue, sleep, and QOL. Blood was obtained for IL-6 measurement. LCA …

Prevalence And Potential Buffers Of Intergenerational Trauma In African American And Latinx Parent-Child Dyads, Kandace Thomas

Dissertations

Buffering intergenerational trauma (IGT) is of great interest to researchers, policy makers and interventionists working to reduce the experience of trauma across generations within the family. IGT has been well studied among families who experienced the Holocaust and there is emerging IGT literature describing the impact of historical events and societal-based adverse experiences across generations. This study expanded upon the IGT literature by exploring and confirming the existence of IGT in a sample of primarily low-income African American and Latinx parents and their 6-year-old children; exploring pre-existing strengths and qualities in parents, such as Contemplative Self-Care (CSC) and Parent Self-Efficacy …

Addressing The School-To-Prison Pipeline Through Critical Self Reflection: A Self-Study Of Courageous And Transformative Leadership, Ernest D. Williams

Dissertations

Research reveals that zero-tolerance policies lead to school suspensions of a disproportionate number of African American students in urban areas (Center for Civil [CCRR], 2015). Suspensions increase student failure rates and dropout likelihood and reduce the ability to graduate on time (Skiba, Arrendondo, & Williams, 2014). Studies have also shown that African American students are suspended three times more than their White American peers and two times more than their Latino American peers (CCRR, 2015). This has impelled federal and local government agencies, community organizations, and educators to question the effectiveness of punitive discipline policies that have marginalized black and …

Yczr, A New Case Of Plp-Dependent Mocr/Gabr Type Transcription Regulator In Klebsiella Pneumonia, Yuanzhang Zheng

Dissertations

Increasing number of genes encoding PLP-dependent transcription regulators, MocR/GabR type regulators, have been identified in various bacterial genomes. However, only a handful of them, including MocR, PdxR and GabR have been studied experimentally. They control different aspects of the bacterial metabolism. Only GabR has reported crystallographic structures. MocR/GabR regulators possess a chimeric structure consisted of a WHTH DNA binding domain and an Aminotransferase-like regulation domain, which can bind PLP as an effector in transcription regulation. Such a chimeric construct presents an interesting case in molecular evolution. The regulation domains of All MocR/GabR type regulators loss their catalytic capacity during evolution …

Lived Experience After Surgery Among Hispanic Adults, Kimberly Sanchez

Dissertations

Background: In 2010, more than 51.4 million procedures were performed in hospitals in the United States. Almost half of all patients had a surgical procedure prior to their hospital discharge and over 80% of patients experienced acute pain after surgery. When acute pain persists for two or more months after surgery, chronic pain develops, costing the United States up to $635 billion annually. Hispanics, in particular, disproportionately experience disparities in pain treatment as they are less likely to receive analgesics. Routine pain assessments conducted using instruments may be inaccurately capturing the pain experience of Hispanic adults. No research has …

The Cooperative Effects Of Channel Length-Bias, Width Asymmetry, Gradient Steepness, And Contact-Guidance On Fibroblasts’ Directional Decision Making, Quang Long Pham

Dissertations

Cell migration in complex micro-environments, that are similar to tissue pores, is important for predicting locations of tissue nucleation and optimizing scaffold architectures. Firstly, how fibroblast cells - relevant to tissue engineering, affect each other’s directional decisions when encountered with a bifurcation of different channel lengths was investigated. It was found that cell sequence and cell mitosis influence the directional choices that the cells made while chemotaxing. Specifically, the fibroblasts chose to alternate between two possible paths - one longer and the other shorter - at a bifurcation. This finding was counter-intuitive given that the shorter path had a steeper …

Magnetic Field Assisted Milli-Scale Robotic Assembly Machine: An Approach To Massively Parallel Swarm Robotic Automation Systems, Yan Liu

Dissertations

The vision of highly parallel, automated manufacturing systems that can build macroscopic products by heterogeneous assembly of many small devices will have a major impact in manufacturing. In this study, a novel milli-scale robotic assembly machine with parallel capabilities, assisted with programmable magnetic field, is developed. The machine prototype consists of a 16x16 array of electromagnets. The dimensions of the electromagnets are 5mm high with an inner diameter of 1.1mm and outer diameter of 2.5mm. All the electromagnets are driven by a 16x16 array of H-Bridges, and an Arduino microcontroller is used to control and program the arrays.

Using the …

Methods To Improve The Remediation Of Polycyclic Aromatic Hydrocarbons (Pahs) In Aerobic And Anaerobic Environments, Brian Wartell

Dissertations

Oil spills occur regularly in terrestrial environments and crude oil can contain many compounds that are highly resistant to degradation. Among these compounds are high levels of polycyclic aromatic hydrocarbons (PAHs) which are not only toxic but can also be carcinogenic and/or mutagenic. The first chapter of this dissertation includes an extensive review chapter on the variables affecting the anaerobic degradation of hydrocarbons, with a particular focus on PAHs. Electron acceptors, electron donors, temperature, salinity, pH all play key roles in determining the possibility effective of effective degradation occurring. Thus, by addressing solutions, such as biostimulation, improving environmental variables for …

Fwer Controlling Procedures In Simultaneous And Selective Inference, Li Yu

Dissertations

With increasing complexity of research objectives in clinical trials, a variety of relatively complex and less intuitive multiple testing procedures (MTPs) have been developed and applied in clinical data analysis. In order to make testing strategies more explicit and intuitive to communicate with non-statisticians, several flexible and powerful graphical approaches have recently been introduced in the literature for developing and visualizing newer MTPs. Nevertheless, some theoretical as well as methodological issues still remain to be fully addressed. This dissertation addresses several important issues arising in graphical approaches and related selective inference problems. It consists of three parts.

In the first …

Functionalized Carbon Nanotubes In Hydrophobic Drug Delivery, Kun Chen

Dissertations

The direct incorporation of carboxylated carbon nanotubes (f-CNTs) into hydrophobic drug particles during their formation via anti-solvent precipitation is presented. The approach is tested using two drugs namely antifungal agent Griseofulvin (GF) and antibiotic Sulfamethoxazole (SMZ) that have very different aqueous solubility. It is observed that the f-CNTs dispersed in the water serve as nucleating sites for crystallization and are readily incorporated into the drug particles without altering crystal structure or other properties. The results show that the hydrophilic f-CNTs dramatically enhance dissolution rate for both drugs. The increased degree of functionalization leads to higher hydrophilicity and therefore faster dissolution …

Deep Learning Methods For Mining Genomic Sequence Patterns, Xin Gao

Dissertations

Nowadays, with the growing availability of large-scale genomic datasets and advanced computational techniques, more and more data-driven computational methods have been developed to analyze genomic data and help to solve incompletely understood biological problems. Among them, deep learning methods, have been proposed to automatically learn and recognize the functional activity of DNA sequences from genomics data. Techniques for efficient mining genomic sequence pattern will help to improve our understanding of gene regulation, and thus accelerate our progress toward using personal genomes in medicine.

This dissertation focuses on the development of deep learning methods for mining genomic sequences. First, we compare …

Cardiac Regenerative Medicine: Insights From Healthy And Diseased Engineered Tissues, Pamela Grace Hitscherich

Dissertations

Cardiovascular disease remains the leading cause of mortality in the United States. Current tissue engineering approaches have fallen short of promoting fully functional cardiovascular cells and the post-myocardial infarction microenvironment is still not well understood. These gaps in knowledge are addressed in this dissertation through the development of in vitro engineered cardiac tissues using electroactive materials to enhance the differentiation of pluripotent stem cell derived cardiomyocytes and through the development of in vitro myocardial inflammation models dedicated to understanding cardiomyocytes and macrophages interactions.

Specifically, piezoelectric poly (vinylidene fluoride-trifluoroethylene) (PVDF-TrFE) supports the attachment and survival of mouse embryonic stem cell derived …

Overcoming Conventional Modeling Limitations Using Image- Driven Lattice-Boltzmann Method Simulations For Biophysical Applications, Olufemi E. Kadri

Dissertations

The challenges involved in modeling biological systems are significant and push the boundaries of conventional modeling. This is because biological systems are distinctly complex, and their emergent properties are results of the interplay of numerous components/processes. Unfortunately, conventional modeling approaches are often limited by their inability to capture all these complexities. By using in vivo data derived from biomedical imaging, image-based modeling is able to overcome this limitation.

In this work, a combination of imaging data with the Lattice-Boltzmann Method for computational fluid dynamics (CFD) is applied to tissue engineering and thrombogenesis. Using this approach, some of the unanswered questions …

Adolescent Female Substance Abuse: Risk And Resiliency Factors, Mary Bemker

Dissertations

Alcohol, tobacco, and other drug (ATOD) abuse is a major social and health concern for adolescent females. For the first time, female use patterns are converging with male patterns among U.S. adolescents. Despite evidence that gender differences could be associated with ATOD origin and precipitating factors, little attention has been directed to female adolescents and ATOD use. Therefore, the purpose of this investigation was to explore the experiences of adolescent females in relation to ATOD risk and resiliency factors present in their lives. This investigation used the Neuman systems model as its theoretical framework. Grounded theory methodology was employed to …

Systematics Of Malesian-Pacific Piper (Piperaceae), Rani Asmarayani

Dissertations

Comprised of ~2400 spp., Piper is a major clade in the magnoliid angiosperms. Three major groups are recognized in Piper, i.e., the Neotropical, Asian and South Pacific. Unlike Neotropical Piper,relationships in the Paleotropical Piper remain enigmatic. This study focused on the Paleotropical Piperwith emphasize on the Malesian-Pacific Piper, Malesia being the center of diversity of the genus in the Paleotropics. The goals are to evaluate relationships within Paleotropical Piper (chapter 1), to evaluate characters, morphological (chapter 1) and stem anatomical (chapter 2), that may distinguish clades, and to investigate the species boundaries focusing on the well-supported …

A New Keynesian, Neo-Institutional Synthesis: Connecting And Explaining Under-Evaluated Risks In Consumption, Trade, And Finance, Todd Barry

Dissertations

The overall research questions addressed in this dissertation are twofold. First, what are the largest, under-evaluated (or unorthodox) factors that cause growth volatility in both the short and long terms? Second, how can one best determine how much risk is being taken across both of these periods, and how can states increase and/or diversify their risks? The principle hypotheses are that finicky consumerism and structural economic changes, undiversified trade, and under-financed investment contribute significantly to growth volatility. Above all, risk taking actions and policies in countries cause economies to fluctuate. Further, the dissertation suggests that the short-term risk characteristics of …

Diffusion, Adoption, And Network Constraint: Can Understanding Personal Networks Of Afghan Women Improve The Adoption 0f Agricultural Innovations, Clair Wilcox

Dissertations

A plethora of case studies conducted in myriad locations find that factors influencing the adoption of agricultural innovation are different, emphasizing the need for locality specific understanding (Waddingon 2014). The diffusion of agricultural innovations may be influenced by the structure of the social system in which that diffusion is taking place. This study investigates the social structure of rural Afghan women, in an effort to determine how they interact and exchange information, and how the personal network structure, and the nature of the women’s interaction influences adoption of innovations. With the objective of framing rural development programs targeting women in …

Stress Response Mechanisms Of Listeria Monocytogenes, Oindrila Paul

Dissertations

Listeria monocytogenes is a Gram-positive, facultative intracellular food-borne pathogen that causes the disease listeriosis. In order to establish an infection, L. monocytogenes must survive multiple stressors encountered within the gastrointestinal tract, including alterations in pH, bile, salt, and oxygen availability. This dissertation focused on understanding the stress response of L. monocytogenes to bile. Bile acts as a bactericidal agent by disrupting the membrane integrity and causing instability to macromolecules like DNA. Thus, a bacterium must be able to maintain its membrane architecture, composition and integrity.

Often times, bacteria will modulate their fatty acid composition in the membrane to cope with …

Hydrogen Bond Mediated Water Structuring, Diffusion, And Elastic Properties In Acrylamide Copolymer Glycohydrogels, April Levu Fogel

Dissertations

Glycohydrogels have recently gained considerable interest as biocompatible and high water content hydrogels that have similar physicochemical nature to the cell membrane, making them ideal materials for targeted biomedical and personal care applications (e.g., drug delivery systems, biosensors, and contact lenses). Regardless of the specific application, water-polymer and water-water hydrogen bonding interactions have been shown to dictate hydration stability and diffusional properties in traditional hydrogel architectures (e.g., crosslinked HEMA). However, due to the development of glycohydrogel materials within the past two decades, most literature focuses on synthetic techniques and general hydration characteristics. Furthermore, scant literature examines the effect of hydrophobically …

Structure-Property Relationships Of Solvent-Cast Organic Thin Films For Model Corrosion Control Coatings, Austin Maples

Dissertations

This dissertation aimed at answering several fundamental questions, specifically: 1) What is the effect of scribing parameters on visual corrosion assessments? 2) What role do molecular weight, residual solvent, and polymer composition have on morphological changes in organic thin films? 3) What alternatives to visual assessments could be implemented to better understand and detect corrosion before macroscopic failure? This research definitively proved that scribes can vary in type and dimensions, and yet are still comparable if the scribe dimensions before exposure are known. Furthermore, it demonstrated that solvents common to a variety of coating industries could become trapped within the …

The Prologue To Management: The Effects Of Historical Anthropogenic Activities On Forest Ecosystems And Current Management Opportunities In Southwestern Illinois, John Timothy Lovseth

Dissertations

Forest disturbance occurs on a wide gradient of selectiveness and creates new growth opportunities for adapted species. Across the spectrum of disturbance, anthropogenic disturbance influences community assembly in the Midwest more than other mechanisms but is its role in shaping and maintaining ecosystems is inadequately considered in most discussions on the historic range of variability (HRV). Forest resiliency is threatened by unprecedented agents of ecosystem change such as invasive species and reduced regeneration potential of native species. Historic anthropogenic disturbance largely resulted in forest conditions which commonly contained high value attributes like heterogeneity across habitat types and landscape diversity, yet …