Digital Commons Network^™

Vigilance Of Certified Registered Nurse Anesthetists, Timothy Finn

Dissertations

Vigilance of certified registered nurse anesthetists (CRNAs) is an integral component of the care they provide patients under general anesthetic. the practice of CRNas is important as the official seal of the American Association of Nurse Anesthetists (AANA). on the seal, Morpheus, the god of dreams, is shown holding the Lamp of Learning, by the light of which he keeps vigil (Hirter & Van Nest, 1995). as the patients remain unconscious under general anesthesia, CRNas remain the patients' eyes and ears remains responsive to their needs. the CRNa must remain attentive to the patient's needs by continuously monitoring the patient …

Elucidating The Targets And Function Of The Mlr Compass-Like Complex During Development, David Joseph Ford

Dissertations

MLR COMPASS-like complexes are highly-conserved epigenetic regulators required for enhancer establishment and subsequent reprogramming during differentiation and development. Mutation of MLR complex subunits in humans is associated with cancer and developmental disorder, yet much remains to be determined concerning both the healthy and disease-altered functions of these complexes. Using the developmental model Drosophila melanogaster, I further elucidate the functions of the MLR complex during in vivo organ development as well as stress response. I characterize the miRNa bantam as a regulatory target of the complex, required for proper tissue patterning during wing and compound eye formation. in the same systems …

"Lifting As We Climb": Black Feminist Action Research With Women Of Color Student Affairs Administrators, Paige J. Gardner

Dissertations

Black feminist action research is a methodology designed by Dr. Gardner to cultivate agency and solidarity among Women of Color student affairs administrators. by engaging participants as co-investigators, Women of Color student affairs administrators' co-constructed knowledge to examine the challenges and barriers experienced in career navigation as well as the strategies used to disrupt oppressive working environments at historically White institutions. in this study the principle investigator and co-investigators explored the following research questions: (1) How do Women of Color student affairs administrators define systems of support? (2) How and why do Women of Color student affairs administrators establish systems …

Identifying Transfer Of Care Gaps: Electronic Health Record Capture Of Perioperative Handoff Communications, Sharon Giarrizzo-Wilson

Dissertations

Transitions in patient care are held together by interdisciplinary handoff communications intended to coordinate the patient's ongoing care requirements. Patients with complexity in care encumber the transfer of care process requiring a higher level of care coordination between the interdisciplinary team (Coleman, 2003; Naylor et al., 2004). While the literature is abundant on the characteristics and quality of handoff communications, it is limited on the requirements of what data is necessary for ongoing care following transfer communications (Galatzan & Carrington, 2018). This dissertation explores the verbal information transferred during Operating Room (OR) to Post Anesthesia Care Unit (PACU) nursing handoff …

Elucidating The Impact And Mechanism Of Hepatitis C Virus Cell-To-Cell Spread, Karina Durso-Cain

Dissertations

Virus cell-to-cell spread has been implicated in the establishment of persistent infection and has been shown to be involved in the transmission of antiviral resistant mutants. However, relatively little is known about how virus cell-to-cell spread impacts infection or the specific mechanisms by which cell-to-cell spread occurs. as such, this dissertation focused on investigating HCV cell-to-cell spread not only to learn more about this medically important virus, but also to determine the broader impact cell-to-cell spread has on viral infection dynamics, identify the cellular factors involved, and perhaps ultimately provide insight into antiviral strategies that might enhance the barrier to …

Optimization Of Microbial Fuel Cells, Norberto M. Gonzalez

Dissertations

Optimization of microbial fuel cells is investigated by utilizing Shewanella oneidensis as a model microorganism. the microbe's ability to grow and use glucose as a carbon source is explored under varying oxygen environments through an offline PMP derivatization method and HPLC analysis. Shewanella growth and glucose utilization is enhanced under aerobic environments; however, under microaerobic environments the addition of ferric iron results in a faster exponential growth initialization. a flavin mononucleotide modified indium tin oxide electrode is prepared and characterized for its usefulness in microbial fuel cells by controlled potential electrolysis, cyclic voltammetry, and electrochemical impedance spectroscopy studies. the electrode …

Utopian Discourse In Contemporary Speculative Fiction, Casey Alan Jergenson

Dissertations

I argue in this dissertation that utopianism is a vibrant form of cultural production in the post-Cold War period, despite the paucity of recent texts depicting €œgood€ societies. Most literary historical accounts of the genre place the decline of the utopian narrative in the early twentieth century, with a brief resurgence in the 1960s and 1970s. Contemporary culture has since become inundated with dystopian and post-apocalyptic visions of the future. If we take this generic distribution at face-value, it seems symptomatic of the utopian idea's retreat from cultural production since the 1980s. Influential critics have resisted this narrative by demonstrating …

Seed Conflicts In Colombia: Ethnorace, Territory, And Violence, Nathalia Hernandez Vidal

Dissertations

This dissertation follows the theoretical approach of the food regime scholarship (Friedmann and McMichael, 1989) to understand the process of privatization of seeds and the social and material processes associated with it, known as the Corporate Seed Regime (CSR). the CSR is a transnational regime of governance over the bios (life) born in the post- World-War II period, imagined and enforced by and through the World Trade Organization (WTO) and the World Bank (McMichael, 2013). in this work, I explain how a corporate seed regime (CSR) has taken form in Colombia. Extant sociological studies on the formation of CSRs explain …

The Shadow-Beast: The Influence Of Critical Consciousness On Resilience Narratives Of Latinx/A/O College Students, Norma Lopez

Dissertations

The purpose of this LatCrit narrative study was to explore how Latinx/a/o students who are first-generation to college make meaning of their resilience in relation to the development of their critical consciousness. the research questions that guided this study were: How do first-generation to college Latinx/a/o students make meaning of their ability to overcome obstacles and persevere in the face of adversity; in other words, how have they constructed their resilience narrative? How does critical consciousness, or understanding their social identity within the context of social, political, and economic forms of oppression, influence their resilience narrative, if at all? Data …

Mortality Salience, Group Membership, & Reciprocity, Fatima A. Martin

Dissertations

This study investigated the effects of mortality salience, group membership, partner's behavior, and adherence to the norms of positive and negative reciprocity. Three-hundredeighty- four participants completed the either a mortality salience or control (where mortality was not salient) induction, a group membership induction, a personal norms of reciprocity scale, one round of a single-shot trust game (a measure of positive reciprocity), and one round of a singleshot ultimatum game (a measure of negative reciprocity). During the trust game, an ostensible interaction partner received a small monetary endowment, and contributed either that entire endowment or less than half of it (keeping …

Neuropsychological Correlates Of Chronic Pain: The Influence Of Anxious Arousal On Cognitive Control, Kelly L. Polnaszek

Dissertations

The present set of studies assessed the relation between neural, psychological, and cognitive mechanisms that have been shown to influence the experience of chronic pain. Specifically, Studies One and Two advanced our understanding of chronic pain by illustrating its unique relationship with anxious arousal and frontal neural activity on inhibitory control difficulties. Study Three focuses on targeting how anxious arousal is distinctly associated with neural correlates of inhibitory control in order to provide a framework that can be applied to individuals experiencing chronic pain. Together, these studies aim to inform the development of evidenced-based interventions that target anxious arousal in …

Distinct Roles For Carbohydrate And Protein Receptors In Coronavirus Infection, Enya Qing

Dissertations

Coronaviruses (CoVs) are common human and animal pathogens. in humans, four endemic CoV species together account for one third of mild respiratory infections worldwide. More severe and frequently fatal respiratory pathologies are caused by recent CoV outbreaks that resulted from occasional zoonotic spillover from animal CoV reservoirs, namely, SARS-CoV in 2002, MERS-CoV in 2012, and SARS-CoV-2 in 2019. Because CoVs threaten global health, any chance of relieving CoV's threat on human populations would rely heavily on our understanding of the mechanistic requirements for CoV tropism, whose major determinant is at the level of viral entry. CoVs have evolved to use …

An Ecological Model Of Transmission Of Depression: Risk And Protective Factors For Mexican Immigrant Mothers And Children Living In Poverty, Anna Maria Ros

Dissertations

Family poverty has been found to predict a number of negative outcomes across a multitude of domains of child development, including health, education, language and cognitive development (Reiss, 2013; Yoshikawa, Aber & Beardslee, 2012). Furthermore, studies examining differing prevalence rates of mental illness depending on socioeconomic status have found that children liv-ing in poverty in the United States are between two and three times as likely to meet criteria for a psychiatric disorder when compared to their higher SES counterparts (McLaughlin et al, 2011; Reiss, 2013). Family poverty has been found to predict internalizing psychopathology risk across childhood and adolescence, …

Developing Caspase-1 Biosensors To Monitor Inflammation In Vitro And In Vivo, Sarah Talley

Dissertations

Inflammation is central to the pathogenesis of numerous human diseases. in many contexts, inflammation drives immune responses which are critical to protection against many pathogens. However, uncontrolled inflammation can drive the pathology of numerous diseases, including neurodegenerative diseases, intestinal disorders, cancer, and autoimmune diseases. a primary mediator of inflammation is the inflammasome complex, which drives the release of inflammatory mediators through caspase-1 activation. Our goal was to develop a tool that would allow us to monitor caspase-1 activation in vitro and ultimately in vivo. to this end, we employed a circularly permuted form of luciferase in which the N- and …

Targeting Epha2 In Hepatocellular Carcinoma: Uncovering Its Therapeutic Potential And Beyond, Hao Wang

Dissertations

Hepatocellular Carcinoma (HCC) remains one of the deadliest malignancies worldwide. One major obstacle to treatment is a lack of effective molecular-targeted therapies. Here, we identify EphA2 receptor tyrosine kinase as a clinically relevant target for HCC. EphA2 expression and signaling is enriched in human HCC and associates with poor prognosis. Loss of EphA2 suppressed the initiation and growth of HCC in vitro and in vivo. Furthermore, CRISPR/CAS9 mediated EphA2 inhibition significantly delayed tumor development in a genetically-engineered murine model of HCC. Mechanistically, we discovered that targeting EphA2 simultaneously suppresses both JAK1/STAT3 and AKT signaling, two separate oncogenic pathways in HCC. …

Movement On The Margins: Exploring The Leadership And Agency Of Women Of Color Student Activists In Predominantly White Higher Education Institutions, Cobretti Williams

Dissertations

The purpose of this phenomenological study was to learn more about the experience of student activism through the eyes, ears, and narratives of women of color in predominantly White higher education institutions. Using an adapted theoretical framework of critical feminist agency, I conducted interviews and focus groups with women of color whom represent the past, present, and future of student activist experiences on campus. Their stories and knowledge affirmed the historical significance of student activism in higher education, and more notably, provided additional critical perspectives towards the development and re-imagination of leadership, agency, and institutional structures in colleges and universities. …

Human Isocitrate Dehydrogenase Enzymes, Joseph Valentein Roman

Dissertations

Mammals have three forms of isocitrate dehydrogenase (ICDH) that each catalyze the oxidative decarboxylation of isocitrate forming alpha-ketoglutarate (AKG), carbon dioxide and reduced pyridine nucleotide. The intra-mitochondrial, citric acid cycle enzyme is ICDH III that exists as a hetero tetramer and uses NAD+ as a substrate. ICDH I and ICDH II are both dimeric enzymes that reside in the cytosol and use NADP+. While, the precise function of the cytosolic ICDHs is not known, active site variants of both ICDH I and II cause a variety of cancers. The R132H variant of ICDH1 is one of the most common alterations. …

Electron Transfer Of Shewanella Oneidensis Mr-1 At Clay-Modified Ito Electrode, Reem Faez Alshehri

Dissertations

Various strategies have been established to enhance the extracellular electron transfer and energy output capability of microbial fuel cells, with the majority being aimed at anode modification. The anode has a significant impact on the electricity generation performance of MFCs because it is in direct contact with the microorganisms. The materials of the anode should be favorable for the bacterial cell and capable to facilitate the electron transfer. Developing of an electrode using low-cost and effective materials assists to enhance the bacterial cell attachment and extracellular electron transfer. This provides a significant improvement in MFC performance. In this study, clay …

Parenting Capacity And Meaning Making Among Survivors Of Intimate Partner Violence, Johanna Engler Barry

Dissertations

This qualitative dissertation used a feminist methodology to explore parenting capacity and meaning making among a purposive sample of 16 female survivors of intimate partner violence (IPV). The two research questions that guided this work centered on how women's exposure to IPV during both childhood and adulthood impacted parenting capacity. All participants for this dissertation experienced IPV during childhood as well as adulthood, and had at least one school-aged child. Participants completed semi-structured in-person interviews during which they were asked a series of questions about their experiences with IPV during childhood and adulthood as well as questions about their parenting …

Cellular And Molecular Mechanism Underlying Alcohol Inhibition Of Bone Fracture Healing, Jonathan Michael Eby

Dissertations

Alcohol consumption is associated with increased risk taking and injuries leading to bone fracture. During bone fracture repair mesenchymal stem cells (MSCs) located at the site of injury differentiate into chondrocytes and osteoblasts to form a fracture callus. While most fractures heal without complication, studies show that alcohol abuse can lead to delayed fracture healing and fracture callus formation in both people and animals. Despite what is known about alcohol consumption in pre-injury pathologies like osteopenia and osteoporosis, the cellular and molecular mechanisms by which alcohol consumption inhibits fracture callus formation following orthopaedic injury remain to be elucidated. Here, we …

Determining The Genetic And Phenotypic Profile Of Cord Blood Derived T Regulatory Cells And The Effect Of Calcitriol On Immune Suppression, Anya Nikolai-Yogerst

Dissertations

Recent outbreaks in vertically transmitted viruses, such as Zika virus and HIV, have rejuvenated interest in fetal and neonatal immune tolerance. Babies are susceptible to vertically transmitted viruses because fetal and neonatal immune systems are considered to be immune-privileged and will tolerate antigens presented to them by their mother. Thus, understanding the mechanisms of this tolerance is important to design effective treatments for infected mothers to protect their offspring. Here, I define a novel Treg, a type of T cell that mediates tolerance, named bidirectional T cells (BiT), found in human umbilical cord blood (UCB). These cells are induced by …

Factors Associated With Diabetic Peripheral Neuropathy And Quality Of Life Among Type 2 Diabetes Patients In Saudi Arabia, Awatef Ibraheem

Dissertations

Evidence has suggested that diabetic peripheral neuropathy (DPN) is associated with decreased quality of life (QoL) and impaired physical and emotional health. About 82% of the diabetic population in Saudi Arabia show signs of DPN. However, it is under-recognized and undertreated. Further, DPN is linked to several clinical and metabolic risk factors. However, there is minimal evidence from Saudi Arabia on the impact of DPN on the individual's QoL. The aims of this current study were to (1) examine the impact of DPN symptoms on functioning, health perception, and QoL and (2) explore the biologic and individual factors associated with …

A Study Of Nones In Brazil And The Usa In Light Of Secularization Theory With Missiological Implications, Jolive R. Chavez

Dissertations

The growth of those who declare themselves to be Nones, or religiously unaffiliated, in Brazil and the USA has been continuously higher than that of the general population. In Brazil, they are the third-largest group in the religious field, behind only Catholics, and Pentecostal evangelicals. In the USA, they are the second largest group, after Protestants as a whole. The Nones, in their diverse groups, are more represented among the youth in both cultures, and reflect the modern and postmodern influences of contemporary secularism, being a product of the process of changing human thought.

This work studies the Nones, in …

Pulmonary And Systemic Pathophysiological Impact Of Silver Nanoparticles: Effects Of Coating, Time, And Dose, Zannatul Ferdous

Dissertations

Nanotechnology is rapidly developing, and engineered materials (ENMs) are utilized in a wide array of end-user products owing to their unique physicochemical properties. Of the various forms of ENMs, silver nanoparticles (AgNPs) have gained much attention for potential uses in commercial applications. Due to their unique features, particularly antimicrobial properties, there had been a surge in the production of AgNPs-based products, hence increasing the potential of both occupational and consumer exposure. Among the various routes of human exposure, pulmonary exposure to AgNPs has received increased interest due to their potential to cause pulmonary injury, cross the alveolar-capillary barrier, and distribute …

Enhanced Label Free Normal And Cancer Cells Classification Using Combined Parametric Modeling And Optical Techniques, Ayshathul Fouzia Abdul Gani

Dissertations

The development of label-free methods for cell classifications has been driven by the importance of early detection and identification of cancer disease. The future point-of-care (POC) treatment methods require rapid and real-time cancer screening techniques. As the labelled methods of cell classification are time-consuming processes and require a large amount of sample preparation along with skilled persons, they do not appear to be suitable for POC treatment methods. This necessitates the importance of such development. The label-free methods incorporate the biophysical properties of cells instead of biomarkers. The optical properties of cells have been frequently utilized for cell classification. This …

Customized Boron And Magnesium-Based Reactive Materials Prepared By High Energy Mechanical Milling, Xinhang Liu

Dissertations

New reactive materials need to be developed having biocidal combustion products. When ignited, such material can add chemical biocidal effects to the common effects of high temperature and pressure. Biocidal combustion products are capable of deactivating harmful spores or bacteria, which can be released by targets containing biological weapons of mass destruction. Research showed that halogens, especially iodine, are effective as biocidal components of reactive material formulations. Recently, magnesium combustion product MgO is also found to have a biocidal effect. Thus, advanced formulations containing both magnesium and iodine are of interest; such formulations are prepared and investigated here.

Reactive materials …

Perivascular Waste Metabolites Clearance In Central Nervous System (Cns), Yiming Cheng

Dissertations

Efficient clearance of interstitial waste metabolites is essential for normal brain homeostasis. Such effective clearance is hampered by the lack of a lymphatic system in the brain, and the cerebrospinal fluid (CSF) is unable to clear large size waste metabolites in the brain. Here, a novel idea that brain arterial endothelium and smooth muscle cells reactivity regulates the clearance of these water-insoluble large size waste metabolites through the perivascular dynamic exchange, and that low dose ethanol promotes this perivascular clearance is proposed.

In Aim 1, the biodistribution of a large size waste metabolite (Amyloid-β protein mimic) in rat perivascular space …

Bio-Inspired Learning And Hardware Acceleration With Emerging Memories, Shruti R. Kulkarni

Dissertations

Machine Learning has permeated many aspects of engineering, ranging from the Internet of Things (IoT) applications to big data analytics. While computing resources available to implement these algorithms have become more powerful, both in terms of the complexity of problems that can be solved and the overall computing speed, the huge energy costs involved remains a significant challenge. The human brain, which has evolved over millions of years, is widely accepted as the most efficient control and cognitive processing platform. Neuro-biological studies have established that information processing in the human brain relies on impulse like signals emitted by neurons called …

Amyloid Proteins And Fibrils Stability, Farbod Mahmoudinobar

Dissertations

Compared to globular proteins that have a stable native structure, intrinsically disordered peptides (IDP) sample an ensemble of structures without folding into a native conformation.One example of IDP is the amyloid-beta(Abeta) protein which is the main constituent of senile plaques in the brain of Alzheimer's patients.Understanding the process by which IDPs undergo structural changes to form oligomers that eventually aggregate into senile plaques/amyloid fibrils may significantly advance the development of novel therapeutic methods to treat neurodegenerative diseases, for which there is no cure to date. This dissertation has two main objectives. The first one is to investigate and identify structural …

Mitochondria Imaging And Targeted Cancer Treatment, Tinghan Zhao

Dissertations

Mitochondria are essential organelles as the site of respiration in eukaryotic cells and are involved in many crucial functions in cell life. Dysfunction of mitochondrial metabolism and irregular morphology have been frequently found in human cancers. The capability of imaging mitochondria as well as regulating their microenvironment is important both scientifically and clinically. Mitochondria penetrating peptides (MPPs), certain peptides that are composed of cationic and hydrophobic amino acids, are good candidates for mitochondria targeting. Herein, a novel MPP, D-argine-phenylalanine-D-argine-phenylalanine-D-argine-phenylalanine-NH₂ (rFrFrF), is conjugated with a rhodamine-based fluorescent chromophore (TAMRA). The TAMRA-rFrFrF probe exhibits advantageous properties for long-term mitochondria tracking of …