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Deficiency Of Adipose Aryl Hydrocarbon Receptor Protects Against Diet-Induced Metabolic Dysfunction Through Sexually Dimorphic Mechanisms, Nazmul Haque

Dissertations

Calorie imbalance due to high energy intake vs. expenditure is considered a major cause of obesity. Molecular mechanisms underlying diet-induced obesity remain unclear due to the complexity of disease progression. The impact of environmental factors contributing to obesity also remains poorly understood. Activation of the xenobiotic sensor, aryl hydrocarbon receptor (AhR), by obesogens may contribute to diet-induced obesity. AhR has the potential to influence lipid metabolism, insulin resistance, and the development of diabetes. Activation of AhR increases the risk of metabolic dysfunction by impairing adipose tissue function. Thus, the hypothesis was that conditional AhR depletion specifically from mature adipose tissue …

Characterization And Genomic Analysis Of Two Escherichia Coli O157:H7 Bacteriophages Isolated From Pigeons, Mohamad I. Alolama

Dissertations

Enterohemorrhagic Escherichia coli, also known as EHEC, is a subset of Shiga toxin-producing E. coli (STEC), and it has recently been identified as one of the principal foodborne pathogens. E. coli O157:H7 is the most important serotype of STEC for its role in causing foodborne illnesses. E. coli O157:H7 could cause various gastroenteritis symptoms such as diarrhea, hemolytic uremic syndrome, hemorrhagic colitis, and thrombotic thrombocytopenic purpura and may cause death. Elimination of E. coli O157:H7 during food processing and storage is a possible solution. Bacteriophages have a significant impact on bacterial populations in nature due to their ability to …

Pathogen Risk Assessment On Arabian Felines In The United Arab Emirates, Laia Montserrat Stefano

Dissertations

The Arabian Peninsula has been known to harbour five species of felines, namely leopards (Panthera pardus), cheetahs (Acinonyx jubatus), caracals (Caracal caracal), sand cats (Felis margarita), and wildcats (Felis silvestris). Currently, their situation in the wild is critical in the region and ex situ breeding efforts are the greatest hope to restore their populations. However, these efforts need to go hand-in-hand with focus on their health, since pathogens are known to be a serious threat to animals under managed care. Globally, the potential pathogens of these five species include viruses, …

Disease Risk Of Reintroducing Primates Confiscated From The Illegal Wildlife Trade, Ana Patricia Mendoza Becerra

Dissertations

Zoonotic infections are easily shared between human and other primates, and thus primate trafficking poses a high risk of introducing zoonotic pathogens into urban environments as well as spreading human-associated diseases into natural environments when infected primates are released. The magnitude of these risks has not been thoroughly studied in the context of primate trafficking and rehabilitation of Neotropical primates. In this dissertation, I address some knowledge gaps in disease risk analysis of reintroducing trafficked primates. In chapter 1, I describe the dynamics, diversity, and composition of live wildlife trafficking in urban markets in Peru. I found these markets are …

The Relationship Among Perfectionism, Perceived Stress, And Coping In Baccalaureate Nursing Students, Amanda Finley

Dissertations

Perfectionism in nursing students is an understudied problem that may affect student and workforce success. Perfectionism has been linked to increased stress and less effective coping styles, as well as depression, anxiety and other psychological alterations. However, it has rarely been studied in nursing students. Studying perfectionism in this population is important because it may increase the likelihood that new nurses will struggle with the stress of practice and it could be a significant factor in early career attrition. The purpose of this descriptive, correlational study was to gain a better understanding of the incidence of perfectionism in a nursing …

Characterization Of Tick And Host-Associated Factors Linked To Induction Of Alpha-Gal Syndrome., Surendra Raj Sharma, Surendra Raj Sharma

Dissertations

Alpha-Gal Syndrome (AGS) is a delayed allergic reaction caused by specific IgE antibodies to the oligosaccharide galactose-α-1,3-galactose (α-gal) present in red meat. AGS is a growing concern globally, with over 34,000 confirmed cases in the United States alone. Studies have linked AGS to tick bites, which sensitizes hosts against α-gal antigens and elevates α-gal specific IgE. This dissertation investigates the role of tick and host-associated factors in AGS development. Microbiome analysis of Am. americanum ticks revealed a comprehensive core microbiome profile and showed that new Fransiella-like endosymbionts dominate and outcompete conventional tick Coxiella endosymbionts. Ticks subjected to tetracycline treatment showed …

Overcoming The Impostor Phenomenon: Exploring The Strategies Special Education Charter School Leaders Used To Cope During The Covid-19 Pandemic, Damaris Ocasio Owens

Dissertations

Purpose: The purpose of this descriptive mixed method study was to explore and describe the coping skills used to overcome the nine behavioral characteristics by special education charter school leaders who identified as experiencing impostor phenomenon (IP) by the Clance Impostor Phenomenon Scale (Clance,1985), while leading the transition to distance learning in response to the COVID-19 pandemic. Methodology: This descriptive mixed-methods research design used quantitative and qualitative data to identify special education leaders with IP and explore the coping skills they used during distance learning. The first phase of the study was a quantitative 20-question survey, the CIPS, used to …

Probing Amyloid-Beta Protein Structure And Dynamics With A Selective Antibody, Shikha Grover

Dissertations

Alzheimer’s disease (AD) is a progressive neurodegenerative disorder. The AD brain is characterized by significant neuronal loss and accumulation of insoluble fibrillar amyloid-β protein (Aβ) plaques and tau protein neurofibrillary tangles in the brain. However, over the last decade, many studies have shown that the neurodegenerative effect of Aβ may in fact be caused by various soluble oligomeric forms as opposed to the insoluble fibrils. Furthermore, the data suggest that a pre-fibrillar aggregated form, termed protofibrils, mediates direct neurotoxicity, and triggers a robust neuroinflammatory response.

Antibodies targeting the various conformation of Aβ are important therapeutic agents to prevent the progression …

Self-Management By Adolescents And Young Adults Following A Stem Cell Transplant, Caroline F. Morrison

Dissertations

A grounded theory study was conducted to better understand the process AYA use to manage their care following a SCT. Specific aims included: 1) to explore self-management facilitators, barriers, processes and behaviors within individual, family, community and healthcare system domains using the pediatric self-management framework to develop initial interview guides; 2) to describe how AYA manage their care regimen post HSCT; and 3) to describe rates of oral medication adherence for AYA post HSCT and how they relate to patterns of self-management.

Impact Of High-Fidelity Simulation On Confidence Level Of Nursing Students In The Care Of Covid-19 Patients, Faith A. Paine

Dissertations

The COVID-19 pandemic impacted the education of nursing students across the world. Nursing students were unable to care for patients with COVID-19 at the beginning and potentially throughout nursing school depending on the facility and nursing school restrictions. It was not known how the participation in a high-fidelity simulation will impact the confidence level of nursing students in the care of patients with COVID-19. A mixed methods phenomenological qualitative and survey design was utilized to determine the confidence level of nursing students after participation in a high-fidelity simulation of a patient diagnosed with COVID-19.

The Relationship Among Spirituality, Self-Efficacy, And Quality Of Life In Adults With Sickle Cell Disease, Maxine A. Adegbola

Dissertations

For the individual with sickle cell disease (SCD), the lifespan is increasing but adults report decreased quality of life (QOL), low self-efficacy, and ineffective coping skills. The care of adult patients with SCD requires a complex multidisciplinary team approach with focus not only on physiological, psychological, and social needs, but also on spiritual needs. Quality of life, spirituality, and self-efficacy have been sparsely and separately studied in individuals with SCD. These three constructs have never been combined in one study in the adult SCD population.

The purpose of this study was to explore and describe the relationships among spirituality, self-efficacy, …

Covid Implications For Southeastern States: A Thematic Analysis Of Influences From Leaders And Health Agencies Social Media Platforms, Jennifer Suber

Dissertations

The problem investigated in this study was the conflicting social media messages between the Office of the President and the two preeminent national health organizations (the Centers for Disease Control and Prevention (CDC) and the National Institute of Allergy and Infectious Diseases (NIAID) at the U.S. National Institutes of Health) regarding the COVID-19 crisis. The purpose of this study was to explore alignment and disconnect in the social media messaging between the Office of the President and the top two health organizations and subsequent state government decisions around the COVID 19 pandemic in the southeast region of the United States. …

Design And Synthesis Of Novel Antibiotics And Anticancer Agents Targeting Pharmaceutically Relevant Enzymes, Sebastian Flieger

Dissertations

Boron neutron capture therapy (BNCT) is a binary radiotherapeutic modality for cancer treatment, in which delivery agents containing 10B atoms are transported into tumor cells and then irradiated with low-energy thermal neutrons. Matrix metalloproteinases (MMPs) are a family of zinc-dependent endopeptidases that are involved in the remodeling and degradation of all components of the extracellular matrix (ECM). Overexpressed levels of MMP activity have been implicated in several disease states including tumor growth and metastasis. We have appended a borane-rich carborane cluster onto MMP-targeting pharmacophores to enable delivery of 10B atoms to a tumor. Herein, we describe the molecular docking experiments …

Photonic Monitoring Of Atmospheric Fauna, Adrien P. Genoud

Dissertations

Insects play a quintessential role in the Earth’s ecosystems and their recent decline in abundance and diversity is alarming. Monitoring their population is paramount to understand the causes of their decline, as well as to guide and evaluate the efficiency of conservation policies. Monitoring populations of flying insects is generally done using physical traps, but this method requires long and expensive laboratory analysis where each insect must be identified by qualified personnel. Lack of reliable data on insect populations is now considered a significant issue in the field of entomology, often referred to as a “data crisis” in the field. …

Twitter Sentiment Analysis: Applications In Healthcare And Finance, Jiali Wang

Dissertations

This research explores the influence of Twitter sentiment on healthcare and finance industries. It assesses how Twitter sentiment and culture measure influence COVID-19 statistics, and it investigates the impact of Twitter sentiment on S&P 1500 stock mispricing. Furthermore, it examines how tweet sentiment predicts major industry returns.

The first part examines how Hofstede’s Culture Dimensions (HCD) and Twitter economic uncertainty index (TEU) relate to COVID-19 infection rate and death rate. The results show certain aspects in HCD, such as power distance index (PDI) and masculinity (MAS) both are negatively and significantly associated with the infection rate, while indulgence (IVR) and …

Daily Rounding To Reduce Catheter-Associated Urinary Tract Infections In A Rehabilitation Facility, Folashade Oluwafunmi

Dissertations

Despite national efforts to reduce catheter-associated urinary tract infections (CAUTIs), healthcare facilities continue to report CAUTI events. The project site reported CAUTI rates above local and national benchmarks even though a CAUTI bundle was in use. The purpose of this quantitative quasi-experimental quality improvement project was to determine if the implementation of daily rounding by organizational CAUTI champions, using the Agency for Healthcare Research and Quality (AHRQ) Comprehensive Unitbased Safety Program (CUSP), would impact CAUTI rates among adult residents in a rehabilitation facility in Northern California for four weeks. Lewin's change theory and the IOWA evidence-based practice model were the …

Investigating The Involvement Of The Tick Vector In The Induction Of Alpha-Galactose Hypersensitivity (Alpha-Gal Syndrome, Red Meat Allergy) In The United States., Gary Crispell

Dissertations

Alpha-gal syndrome (AGS or sometimes called red meat allergy) is a result of the development of specific IgE antibodies to the oligosaccharide galactose-α-1,3-galactose (α-gal) after a person has had exposure to tick bites. This dissertation investigates four common tick species found in North America: the lone-star tick (Amblyomma americanum), the Gulf-Coast tick (Amblyomma maculatum), the American dog tick (Dermacentor variabilis), and the black-legged tick (Ixodes scapularis) for the presence of α-gal by utilizing a combination of immunoproteomic approaches and carbohydrate analysis techniques.

Anti-α-gal IgM antibodies (M86) were used in immunoblotting to detect …

Characterization Of Innate Immune Responses In Mouse Trophoblast Stem Cells And Their Differentiated Cells, Mona Fendereski

Dissertations

The blastocyst is the preimplantation stage embryo consisting of two major components: the inner cell mass (ICM) and the trophectoderm (TE). The ICM forms the fetus, whereas the TE contributes to the placenta development. Previous studies have demonstrated that human and mouse embryonic stem cells (ESCs) derived from the ICM are deficient in expressing type I IFNs in response to viral infection. This study investigated the innate immune responses in mouse trophoblast stem cells (TSCs) and their in vitro differentiated trophoblasts (TSC-TBs). I report that, unlike ESCs, TSCs have a functional IFN system. They can express and respond to type …

Lessons Learned From Emergency Remote Teaching And Learning In A Suburban High School, Stephen Beauchamp

Dissertations

The purpose of this mixed methods study was to examine the experience of teachers and students from a Midwest, mid-sized suburban high school during Emergency Remote Teaching (ERT) stemming from the COVID-19 pandemic. The researcher surveyed teachers and students about the challenges, benefits, and what they plan to carry forward post-pandemic. Because the teachers and students, prior to and over the course of the pandemic experienced several schedule options, preferences as to which schedule type they preferred were surveyed. Schedule types included Traditional, Block, Hybrid, or Remote schedules. Results indicated that for teachers, ERT was a time of learning and …

Genetic And Environmental Drivers Of Microbial Colonization In Wild Birds, Sage Rohrer

Dissertations

Wild birds are teeming with microorganisms, ranging from commensal bacteria to eukaryotic parasites. These microbes impact host health in diverse ways; some long-term residents may aid digestion or provide immune system training, while others at times may induce disease. However, the factors driving the varying colonization patterns seen across taxa are still not fully understood, and wild avian populations are particularly understudied when compared to mammalian or domesticated systems. This work examines the relative importance of genetic and environmental factors driving microbiome composition and function in wild birds. We collected fecal and blood samples from Galapagos penguins (Spheniscus mendiculus …

Hiv And Early Life Stress On Neuroimaging And Risky Behavior, Paola Garcia Egan

Dissertations

This study examined the interactive effects of early life stress (ELS) and HIV on brain morphometry, diffusion-basis-spectrum-imaging (DBSI), risky decision-making, and sex-risk behavior. 122 people with HIV (PWH) and 113 people without HIV (PWoH), free of major psychiatric illness and neurological confounds, were stratified into high (≥ 3 events) vs. low (< 3 events) ELS [PWoH/low ELS (n = 57), PWoH/high ELS (n =56), PWH/low ELS (n = 43), PWH/high ELS (n = 79)] and underwent structural magnetic resonance imaging, DBSI, neuropsychological, and risky-behavior assessment; all PWH were virologically controlled. Compared to PWoH, PWH had smaller orbitofrontal cortex (OFC), parietal lobes, insula, caudate and anterior cingulate. No ELS effects were detected in volumetric measures. Significant interactions were found between HIV serostatus and ELS on the OFC and on cellularity of the inferior fronto-occipital fasciculus after multiple comparisons adjustment. Specifically, PWH/high ELS exhibited significantly smaller OFC and PWoH/high ELS show significantly larger OFC than the other groups. PWoH/high ELS exhibited higher DBSI cellularity (neuroinflammation proxy) of the inferior-occipital-fasciculus compared to PWoH/high ELS. Regardless of HIV status, executive function moderated the relationship between the OFC and sex-risk behavior such that individuals within the sample who performed above average on a measure of executive function and had a larger OFC reported fewer sex partners in past six months than individuals with smaller volumes. No interaction was found between HIV serostatus and ELS on risky behavior measures. Clustering analyses defined ELS subgroups in PWH that were determined by demographic characteristics, duration of infection, recent CD4+ T-cell count, nadir CD4+ T-cell count and high/low ELS.Even in PWH that are virologically controlled, without major current psychiatric comorbidities, there is evidence of a synergistic impact of ELS and HIV on OFC volumes. Higher volumes in the OFC were detrimental when associated with lower executive function scores or advantageous when associated with higher executive function. Findings suggest that ELS is associated with different brain signatures among PWoH and virally suppressed PWH. However, ELS was not directly associated with risky behaviors, and subgroups in PWH were characterized by demographic variables, past substance use and HIV clinical variables.

The Relationship Among Maternal Characteristics, Maternal Dietary Self-Regulation, And The Dietary Intake And Body Weight Status Of Two To Five-Year-Old Children, Andrea Bushaw

Dissertations

Eating preferences and practices are established early in life, yet we have little knowledge about how mothers make food decisions on behalf of their children. These decisions, however, lay the foundation for their child's lifelong food preferences and eating behaviors. Almost one-third of all children in the U.S. are overweight or obese putting them at high risk for becoming an overweight or obese adult. To make a difference in the ongoing obesity epidemic, we must better understand the relationship among characteristics of the mother who controls the young child's access to food, food choices, and feeding practices. This knowledge will …

A Mixed-Methods Study Examining The Impact Of Incentivized Medical Insurance Premiums On Wellness Program Participation And Results At A Four-Year, Private, Midwestern University, Candace L. Terry

Dissertations

The purpose of this mixed-methods study was to examine the impact that incentivized medical insurance premium rates have on wellness plan participation and aggregate wellness results at a Midwestern private, four-year university. Leveraging the Health Belief Model, the researcher sought to compare aggregate wellness results and wellness program participation before and after the medical insurance premium incentive program was implemented. The researcher utilized Likert-scale questions followed by open-ended questions to gain insight into participants’ perceptions of the wellness incentive program. The quantitative portion of this study applied Thorndike’s theory of connecting “A specific response is connected to a specific stimulus …

Infertility Characterization, Factors Associated With Primary Infertility, And Seroprevalence Of Genital Infections: A Cross-Sectional Survey In Abu Dhabi, United Arab Emirates, Noor Motea Abdo

Dissertations

Worldwide, infertility affects 8%–10% of couples and it could be primary or secondary. In the United Arab Emirates (UAE), one in every six couples are facing difficulties in conceiving. However, there is a noticeable lack of epidemiological studies and empirical data characterizing populations seeking infertility treatment in the UAE. Therefore, to inform health policy planning, evidence-based data is required to understand the most common type of infertility and the burden of exposure to genital pathogens. To characterize fertility-treatment-seeking patients, investigate factors associated with primary infertility, and to estimate the seroprevalence of six pathogens known to be transmitted genitally and associated …

Synthesis Of Novel Dna-Binding Polyamides To Prevent Cancer-Related Gene Overexpression, Huy Q. Nguyen

Dissertations

Pyrrole-imidazole polyamides (PIPs) are nanomolecular compounds designed to fit in the tight space of DNA minor grooves. As analogs of Netropsin and Distamycin A, PIPs are specifically designed to recognize the base pairs of DNA sequences. PIPs have many biological applications, such as regulating gene expression, biochemistry pathway regulations, and suppressing the development of cancer cells. SETMAR gene is the chimeric fusion of the SET domain with the mariner transposase. Its protein (Metnase) has functions involving DNA repairs in the NHEJ pathway, regulating gene expression, DNA decatenation, etc. Despite not having an active transposable element, SETMAR still has an unknown …

Elucidating The Mechanisms Underlying How Vmp1 Regulates Inflammatory Responses, Stephanie Zack

Dissertations

autophagy, inflammation, mitochondria, NLRP3 inflammasome, SERCA, VMP1

Elucidating The Mechanisms Of Immune Regulation By Bacillus Subtilis, Jesus Zamora-Pineda

Dissertations

bacillus subtilis, exopolysaccharides, inflammation, probiotics, tlr4

Health-Care Seeking Behaviors Of Puerto Ricans With Diabetes Mellitus Who Live In South Florida: An Exploratory Study, Laura N. Gonzalez

Dissertations

Latinos are the fastest growing minority population in the United States (U.S.) and have the worst access to health care of any ethnic group. The chronic disease of diabetes is twice as common in adult Latinos as in non-Latino whites, and the risk of death related to diabetes is twofold. Reasons for this disparity have yet to be clearly identified. This study had two purposes: 1) to explore cultural beliefs regarding health-care seeking behaviors in Puerto Ricans with diabetes who live in South Florida; and 2) to examine Puerto Ricans' perceptions about their health-care providers. The cultural phenomena of interest …

Examining The Effect Of Gratitude On Intolerance Of Uncertainty And Loneliness, Adam John Paul Mackenzie

Dissertations

Loneliness has been demonstrated to have significant negative effects on overall well-being in the general population. Previous literature has demonstrated a significant association between loneliness and various demographic variables such as gender, age, educational attainment, relationship status, living arrangements and income. Because of the unique impact that the disease caused by the coronavirus, COVID–19, has had on a global scale in relation to physical distancing and increasing physical and psychological distress, the purpose of this study was to examine the role that gratitude played in the relationship between intolerance of uncertainty and loneliness among the general adult population of the …

Implementation Of An Evaluation Of Davita Clabsi Protocol To Reduce Bloodstream Infections In Acute Hemodialysis Patients, Emmy Le

Dissertations

Abstract

Problem: Central line-associated bloodstream infection (CLABSI) is the primary cause of morbidity and mortality in End-Stage Renal Disease (ESRD) patients on hemodialysis. It is associated with severe medical conditions, prolonged hospitalization, and financial burden. Despite its convenience and simple operation, central venous catheter (CVC) account for 70% of all CLABSI occurrence and increase the risk for septicemia and death.

Methods: This quality improvement (QI) project utilized a descriptive observational pilot design to identify the effect of DaVita CLABSI Protocol on CLABSI occurrence rate. Quantitative data was collected retrospectively and prospectively via electronic medical record (EMR) review before and …