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An Evaluation Of Health Behavior Change During A General Education Health Course, Lynn M. Merklin

Dissertations

Problem. There have been no studies as to the effectiveness of the one-credit general education health course at Andrews University, Fit for Life, in promoting the adoption of healthy lifestyle behaviors. Because behavior change is a process that occurs over time, any measurement of student health behaviors must be sensitive enough to detect the early stages of behavior change as well as the behaviors themselves.

Method. The purpose of this study was to determine the degree to which students enrolled in Fit for Life adopted the health behaviors promoted in the course, evidenced by progress through the stages …

Phospholipase D Signaling In T Cells, Uma Chandrasekaran

Dissertations

Antigen stimulation of T lymphocytes induces the activation of phospholipase D (PLD) signaling. Phospholipase D (PLD) is a phosphodiesterase that catalyzes the conversion of phosphatidyl choline (PC) to phosphatidic acid (PA). PA is an important lipid second messenger and is known to mediate a variety of cellular functions. However, the specific role of PA in T lymphocytes has not been established. Previous studies indicated differential requirement for TCR induced PLD signaling in regulatory and non-regulatory T cells. Inhibition of TCR induced PLD signal preferentially suppressed the growth of non-regulatory T cells while allowing the proliferation of regulatory T cells in …

B Lymphocyte Development In Galt, Venkata Arunachalam Yeramilli

Dissertations

In rabbits, the primary antibody repertoire is generated in the gut-associated lymphoid tissues (GALT), where bone marrow (BM)-derived B cells undergo a proliferative expansion and somatically diversify the immunoglobulin genes. Unlike in other species, B lymphopoiesis in rabbit arrests a few months after birth, and it is unclear how the peripheral B cell compartment is maintained when there is no influx of newly-made B cells from the BM.

For my dissertation, I investigated how B cells develop in the GALT of rabbits, and how they are maintained in adults after the arrest of lymphopoiesis. To identify cellular signals that promote …

Ethanol Impairs Mechanisms Of Macrophage Phagocytosis And Cytokine Production, John Karavitis

Dissertations

Extensive evidence indicates that alcohol (ethanol) consumption affects human health by altering normal physiological functions of the immune system. This study investigated the effect of a single in vivo exposure of macrophages to clinically relevant levels of ethanol (1.2 and 2.2 g/kg). Following 3 hour exposure, both doses of ethanol decreased ex vivo TNFα production by splenic and alveolar macrophages (AMs). Interestingly, the higher dose of ethanol resulted in sustained suppression of LPS-induced TNFα production at 3 and 6 hours post ethanol administration, as well as decreased IL-6 and IL-12 production after 6 hours, compared control treated groups. LPS or …

Characterization Of Tram, A Required Bacteroides Fragilis Conjugal Apparatus Protein That Interacts With The B. Fragilis Putative Coupling Protein Bcta And The B. Fragilis Relaxase Protein Bmph, Mai Thi Thanh Nguyen

Dissertations

Bacteroides spp. organisms, the most prominent bacteria of the human colon, are reservoirs of antibiotic resistance genes which promiscuously transfer within and from the genus via conjugation. The B. fragilis clinical isolate LV23 harbors a 37kb conjugative transposon, BTF-37. Two important conjugal apparatus proteins encoded by BTF-37 are BctA, a predicted coupling protein (CP) that powers DNA translocation, and ORF7 (TraM). In this study, we demonstrated that TraM exhibits characteristics of a required conjugal apparatus protein including 1) upregulated expression under conjugation conditions; 2) localization to the bacterial inner membrane; 3) interaction with the putative coupling protein BctA and the …

Quality Of Life And Health Outcomes In Overweight And Non-Overweight Children With Asthma., Amy Becker Manion

Dissertations

Over the last two decades the number of children and adolescents who are overweight has more than doubled. Currently, an estimated 18 percent of children and adolescents ages 6-19 years are overweight. Following this trend in childhood overweight, there has been an alarming increase in the number of children with asthma who are overweight. The increasing trend in both asthma and overweight has led to the suggestion of a causal relationship between the two. Childhood overweight has been found to have a profound negative impact on quality of life (QOL), yet there is a dearth of research regarding the impact …

Experimental Population Dynamics Of Amyloodiniumocellatum In The Spotted Seatrout, Cynoscion Nebulosus, And The Red Snapper, Lutjanus Campechanus, Ignacio Masson

Dissertations

Amyloodinium ocellatum is a parasitic dinoflagellate that infects warm water marine bony fishes and causes high mortalities in aquaculture settings. It has three life history stages: the feeding trophont, the reproductive tomont, and the infective dinospore. This dissertation describes the characteristics of A. ocellatum infections in juvenile spotted seatrout, Cynoscion nebulosus, and red snapper, Lutjanus campechanus, studies the survival and fecundities of the stages in the life cycle of the parasite and presents a population model for A. ocellatum.

At 25°C and 33 ppt, the peak of trophont detachment occurred on day 2 postinfection in spotted seatrout and day 3 …

Empirical Essays On The Impact Of Health-Aid On Health Outcomes, Elsy Thomas Kizhakethalackal

Dissertations

This dissertation consists of three essays that empirically explore the impact of multilateral health-aid on health outcomes like infant mortality rate (IMR) and incidences of an infectious disease, Tuberculosis, in developing economies. The first essay uses parametric and semiparametric mean regressions (additive and non-additive specifications) to capture the impact of education and health-aid on the IMR, after controlling for other covariates. Both specifications confirm education as an important factor in reducing IMR. However, the effect of health-aid on IMR is not significant. In our additive model, we do see a threshold level of health-aid after which the impacts of health-aid …

Gender And Ovarian Hormone Effects On The Relative Contribution Of Chromaticity To Brightness, Brian Keith Foutch

Dissertations

PURPOSE: The chromatic contribution to brightness perception was compared in males and females. METHODS: Direct brightness matching (DBM) and heterochromatic flicker photometry (HFP) were used to measure relative luminous efficiency, and DBM/HFP ratios were predicted to be higher for females than males on repeated measures and for each "primary" color. No gender differences were predicted in DBM or HFP measures. Within-females effects of estradiol (E2) and progesterone (PG) levels, contraceptive use, and menstrual cycle phase were also investigated. It was expected that E2 would directly predict DBM/HFP ratios and that PG would antagonize that relationship. Based on that prediction, DBM/HFP …

Maternally Derived Antibodies In Avian Eggs And Offspring: Ecology, Life History, And Development, Brianne Ashley Addison

Dissertations

Mothers can alter offspring phenotypes through a variety of indirect effects, including the deposition of nutrients, hormones, and defense proteins in to the egg. Defense proteins, and antibodies in particular, may be tremendously important for neonatal defense against pathogens and the direction of resources into growth rather than immune responsiveness. Moreover, maternally derived immunoglobulins have been proposed to have an imprinting effect on the development of humoral immunity. In my dissertation, I explored a variety of ecological, life history, and developmental factors that could contribute to the evolution of yolk antibody allocation in a variety of avian species. In the …

Unlocking Doors For The Locked-Out: How Can Community Colleges Help To Demolish Barriers, Build Bridges, And Transition Male Ex-Offenders Into The Workforce, Jeannette Collins-Molden

Dissertations

Over 670,000 adult prisoners were released in 2004. It is estimated that by 2010 over 1.2 million inmates will be released annually. The purpose of this study was to explore (a) barriers that male ex-offenders encounter in their pursuit of entering the workforce and (b) various types of education and support services that community colleges could provide in order to assist this marginalized population in overcoming these barriers. This qualitative case study was conducted at a community college offering programs that attracted a population of at least 50% male ex-offenders. Six male ex-offender students, four of the case study institution …

Determinants Of Risky Sexual Behaviors Of Kenyan Immigrant Men In The Us And During Visits In Kenya, Japheth K. Kaluyu

Dissertations

Kenyan immigrant men originate from sub-Saharan Africa with the highest HIV prevalence in the world. The study hypotheses were: 1) Attitude towards condom use differs between Kenya and the US, 2) Condom use behavior with primary sex partners differ between Kenya and the US, 3) Condom use behavior with casual sex partners differ between Kenya and the US, 4) Sexual behaviors with casual partners differ between Kenya and the US, 5)Age affects risky sexual behaviors in the US, 6) Age affects risky sexual behaviors during visits in Kenya, 7) Income affects risky sexual behaviors in the US, 8) Income affects …

Development And Psychometric Evaluation Of The Provider Response To Emergency Pandemic (Prep) Tool, Linda Suzzanne Good Phd

Dissertations

Background: History and science would suggest that a worldwide influenza pandemic is near and its implications are on the minds of healthcare workers (HCWs). Previous studies revealed that HCW have loss-related fears and concerns associated with working during a disaster, especially one with a biologic component. Most healthcare organizations have well-crafted disaster plans in place; however, these plans often rely on the assumption that HCWs will report to work as usual, which may not be the case. Objective: The purpose of this study was to determine if HCWs' fears and concerns are a predictor of their willingness to report to …

Domestic Violence Rights Movement In Tanzania: An Exploration, Flora Peter Myamba

Dissertations

In Tanzania, the involvement of a social movement to addressing domestic violence is fairly recent (early 1990s) (Green, 1999; Hirsch, 2003; Michau, 2002; TAMWA, 1999). As has historically been the case in most African countries, domestic violence has remained largely invisible, being considered a normal custom, practice and tradition (Green, 1999; The New York Times, 2005; TAMWA, 1999). Efforts to address it have been regarded as shameful and pointless (TGNP, 1993). Indeed, cultural mores remain very contentious with regard to the activities of the Tanzanian domestic violence rights movement (DVRM). It is this conflict between the movement's efforts to educate …

Mezirow's Transformational Learning Theory And Alternative Health Therapeutics Of Mind, Body, And Spirit, Lewis Edward Blackwell

Dissertations

An illness, like prostate cancer, brings crisis to our experience and confuses the structure of our reality as well as disturbs our meaning which is upheld by interpretive imagery. Hence, recovery involves a learning experience that consists of a reflective, educational, and spiritual process to rebuild and reconstruct meaning through the use and transformation of our images and themes to create meaning. The purpose of the research was to investigate the meaning of the illness experience through the lens of transformational learning theory. As such, one-on-one interviews were conducted with 10 African American men who lived in large metropolitan areas …

Characterization Of The Ap-1 And Nf-Kappa B Transcription Factors In The U-87 Mg Astrocytoma Cell Line, Denise L. Smith

Dissertations

Growth factors, signaling molecules and transcription factors are frequently mutated in cancer and platelet-derived growth factor (PDGF) has been shown to be over-expressed in most gliomas. In the astrocytoma cell line U-87 MG, the over-expression of PDGF has been shown to lead to over-expression of transcription factors activator-protein-1 (AP-1) and nuclear factor-kappa B (NF-κB), and it is believed that the individual subunits of AP-1 (c-Jun, JunB, JunD, c-Fos, FosB, Fra-1, and Fra-2) and NF-κB (c-Rel, p50, p52, p65, and RelB) play different roles in proliferation, survival and differentiation in the U-87 MG cell line.

To assess these different roles, transient …

The Role Of Msa In The Global Regulation Of Virulence In Staphylococcus Aureus, Vijayaraj Nagarajan

Dissertations

Staphylococcus aureus is an important pathogen causing life threatening diseases in humans. Previously we showed that msa modulates the activity of sarA (Staphylococcal accessory regulator), which is one of a major global regulator of virulence in S. aureus. The objective of this study is to characterize the role of msa (Modulator of SarA) in the global regulation of virulence in S. aureus. Structure and function predictions were done using several computational tools and approaches to understand the nature of msa. A novel S. aureus microarray meta-database (SAMMD) was designed and developed to compare and contrast other transcriptomes with msa transcriptome. …

The Theory And Practice Of Drug Courts: Wolves In Sheep Clothing?, Kristen E. Devall

Dissertations

This dissertation is a case study of an adult drug court in a medium-size Midwestern city. The primary impetus behind the creation of the drug court model was the partial recognition that the "get tough" approach to crime and the "war on drugs" was ineffective in "solving the United States' drug problem. Drug courts represent an integration of a public-health approach and a public-safety strategy of fighting crime and administering "justice." The bulk of the extant research regarding drug courts addresses one central question: "Do drug courts work?" Researchers and evaluators alike have attempted to answer this question over the …

Towards Development Of A Fully Synthetic Conjugate Vaccine: Investigation Of Structural Analogs Of Streptococcus Pneumoniae Serogroup 6, Archana R. Parameswar

Dissertations

Involvement of complex glycostructures in a variety of damaging and healing processes has already been acknowledged by development of carbohydrate-based vaccines and therapeutics. The bacteria Streptococcus pneumoniae (SPn) have become one of the most frequent causes of pneumonia, bacteremia, and meningitis in the elderly, immunocompromised, and, especially, in young children. SPn has one of the largest public health and economic impacts amongst all bacterial infectious diseases. Over 2 million children die annually worldwide due to pneumonia, accounting for almost 20% of deaths under age five with more than half of these deaths attributed to SPn. Amongst over ninety elucidated SPn …

Spirituality And Symptom Self Management Of Osteoarthritis, Lois M. Kannan Phd

Dissertations

Healthcare providers need to identify holistic self-care techniques that are endorsed by patients as effective strategies for symptom management of osteoarthritis, which is the single most common cause of disability in older adults. Research in this area may direct the development of interventions to ensure that older adults are afforded every opportunity to remain active and productive, with an improved health related quality of life, despite age related musculoskeletal conditions. The purpose of this study was to describe the relationships among symptom experience, symptom management, and symptom outcome based on spiritual well-being. A quantitative, descriptive, correlational, and noninterventional study design, …

That's Life:An Examination Of The Direct Consequences Of Life-Sentence Imprisonment For Adult Males Within The Irish Prison System, Clara Geaney

Dissertations

Although Ireland compares relatively well in terms of international crime rates, there has been an increase in the number of prisoners serving life-sentences in the Republic. The current system for managing life-sentence prisoners in this jurisdiction is that they are likely to earn their temporary release after having served about fifteen years in prison. However, there is limited research in Ireland on the effects of imprisonment, and certainly for life-sentence prisoners, criminology in the Republic has failed to examine the issues faced by this group at all. Very little is understood about the coping mechanisms specific to life-sentence prisoners; the …

Mobile Information Communication Technology For Crisis Management : Understanding User Behavior, Response And Training, Elizabeth Avery Gomez

Dissertations

SMS text-messaging is an interoperable communication vehicle known to be dependable for mass media alert notifications in crisis management. SMS text-messaging also offers potential as one viable two-way communication alternative for field responders in crisis response. Both continuously changing mobile information communication technologies and the importance of precise information exchange constitute a need for communication protocol training and practice. This study introduces a technology-mediated training technique based on speech act and communicative action theories. These theories are used to inform the design of a baseline measure for task performance improvement and to suggest a model to predict communication readiness. Because …

The Effect Of Chloramphenicol On Bb88 Murine Erythroleukemia Cells, Peter K. W. Harris

Dissertations

DNA microarrays can be used to measure genome-wide transcript levels. These measurements may be useful in understanding cellular changes induced by a chemical agent. In this study, Affymetrix microarray technology has been used to study the effects of chloramphenicol, an antibiotic that inhibits bacterial and mitochondrial protein synthesis, on the transcription profile in mammalian cells. Transcript levels in BB88 murine erythroleukemia cells treated with 50 micromolar ( mu M) chloramphenicol, a concentration shown to inhibit BB88 proliferation, are measured. Using total RNA from treated cells, biotin-labeled cRNA was prepared and hybridized to Affymetrix U74Av2 microarrays. From these hybridizations, expression levels …

Comparative Immune Function In Wild Birds, Kevin David Matson

Dissertations

Over the last several decades, interest in quantifying immune function in comparative studies of wild animals has grown appreciably. Now, the field of ecological immunology is undergoing a transition, and ¿second generation¿ studies are being designed and carried out. With a greater appreciation of the complexity of immune systems, these second generation studies are commonly distinguished from their antecedents by making comparisons using multiple assays and including multiple species. I worked to advance this transition by developing novel approaches to comparative immunology, exploring the interrelationships among indices of immune function, and applying multiple indices to a question of comparative avian …

Navy Nurses' Experiences During Operation Unified Assistance Aboard The Usns Mercy: A Grounded Theory Study, Angelica L.C. Almonte Phd

Dissertations

On December 26, 2004, the most powerful earthquake in 40 years erupted under the Indian Ocean triggering a deadly tsunami that devastated 11 Asian and African countries and killed more than 280,000. The US Navy responded through Operation Unified Assistance (OUA). In a historic first on the hospital ship USNS MERCY, the U.S. Navy deployed one team with members from the Navy, the U.S Public Health Service, a non-government organization (NGO), and a civilian mariner crew to provide humanitarian aid. Despite these efforts, there is a dearth of scholarly research work published on nurses' experiences during the disaster. The purpose …

What Are They Saying: Voices From The Inner City? Lived Experience Of Inner City African American Adolescents With Asthma, Othello Childress Phd

Dissertations

The purpose of this study was to investigate the lived experience of African-American adolescents with asthma who reside in an inner city community. The research in this area of interest is sparse, yet the morbidity and mortality rates related to asthma within the African-American population are greater than the general population. This study offers the experience of living with asthma from the perspective of the young African-American adolescent. Using phenomenological methodology, the nurse researcher selected 13 African-American adolescents; aged 12 to 15, who reside and attend middle school in the inner city. All of the participants had a confirmed medical …

Molecular Analysis Of The Effects Of Atrazine On Xenopus Laevis Frogs, Ronald Edward Celestine

Dissertations

Atrazine, the most commonly used herbicide in the U.S., has been implicated as one of the factors that may be causing the worldwide decline of amphibians. First, I examined effects of atrazine on laboratory raised Xenopus laevis tadpoles, treated from 5 days post fertilization to NF stage 65/66 with a concentrationof 25 ppb of atrazine. This study showed no significant difference in body weight, length and metamorphic rate.

Second, I used DNA microarrays to examine mRNA expression levels of genes in atrazine treated and control Xenopus laevis tadpoles. I found significant changes in gene expression levels of 18 genes. These …

Providing Uninsured Adults With Free Or Low-Cost Primary Care: Does It Influence Their Use Of Hospital Emergency Departments?, Anne G. Zahradnik

Dissertations

This study analyzes one component of the health care safety net to determine whether or not being enrolled in a free or low-cost primary care physician access program subsequently affects emergency room utilization by uninsured adults ages 18 through 64. Those individual decisions are analyzed from both public goods and rational choice schemas. Additionally, physician access programs of different formats (a low-cost physician referral program and a freewalk-in clinic) are analyzed and compared for relative effectiveness. The study is a quantitative analysis of more than 40,000 individual patient records rather than relying on qualitative patient recall or on analyzing broad …

Healthcare Encounters Of Formerly Incarcerated Women: A Grounded Theory Study, Karen Sue Hoyt Phd

Dissertations

The adult correctional population in the United States soared to nearly 7 million people (Bureau of Justice Statistics [BJS], 2005). Over 2 million individuals were housed in prisons or jails in the United States. Nearly 7 percent (6.9%) were women (BJS, 2005). Recent trends in the adult correctional population suggest that there has been a stark increase in the number of formerly incarcerated women in the United States. The purpose of this research was to explore how formerly incarcerated women perceived their healthcare encounters. The aims of this study were to answer the following questions. How did formerly incarcerated women …

Evolutionary Epidemiology Of Endemic Gal¿Pagos Birds And Their Parasites, Noah Kerness Whiteman

Dissertations

In order to better understand parasite diversification, I went to the Gal¿pagos Islands to study the ecology and evolution of a model bird-parasite system, which included four phylogenetically independent ectoparasite lineages infecting the Gal¿pagos Hawk (Aves: Buteo galapagoensis). The parasites comprised two lice (Insecta: Phthiraptera: Amblycera, Ischnocera), a lousefly (Diptera: Hippoboscidae) and an avian skin mite (Acari: Epidermoptidae). Ultimately, my goal was to examine ectoparasite evolutionary epidemiology and disease susceptibility in relation to the host¿s ecological and colonization history. At the outset, I hypothesized that parasite natural history was key in influencing the coalescent process. Accordingly, I found differences …