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High Aspect Ratio Electrodes For High Yield Electroporation Of Cells, Anil B. Shrirao

Dissertations

Electroporation is a widely used process in cell biology studies. It uses an electric field to create pores on the cell membrane in order to either insert exogenous molecules inside the cells or disrupt the cell membrane to kill the cells. Current micro-fluidic electroporation devices use the planar electrodes situated at the bottom of a microchannel. These planar electrodes i) require a high voltage and ii) generate a nonuniform electric field which result in low yield of the electroporation. The standard silicon microfabrication technologies are not suitable to fabricate non-planar electrodes required to increase the yield of electroporation.

In this …

Biofunctional Coatings Using Direct-Write Fabrication Technique For Surgical Implant Devices, Jessica L. Perkins

Dissertations

Surface modification of biomaterials is of critical value to attain desired functionality of biomedical devices and implants. Many of the conventional manufacturing methods used for the fabrication of thin film coatings lack the ability to precisely dispense biological compounds without compromising its chemical integrity. This research investigates the use of direct-write inkjet technique for the deposition of multi-layer coatings of biodegradable polymers. The Direct Write inkjet method provides selective deposition and patterning capability for depositing multi-material coatings on biomaterials for a vast array of surgical implant devices (e.g. stents for cardiovascular applications and orthopedic implants). In the first phase of …

Changes In Perceived Self-Efficacy And Attitude Toward Science And Teaching Science In Elementary School, Betsy Ann Sullivan

Dissertations

This study was developed in an effort to ascertain if a proposed biological laboratory curriculum as developed and modeled by the instructor would affect the attitudes and perceived self-efficacy towards science, science teaching and ability to learn science of pre-service elementary teachers. Self-regulated learning (SRL) strategies were incorporated as the variation. Attitudinal topics investigated were the perceived ability to learn science and to teach science.

Students in one biology for non-science majors' biology laboratory class at the University of Southern Mississippi participated in this case study. The group participated in the modified laboratory section which utilized SRL activities, including reflections …

Comparative Genomic Analyses And Expression Of A Set Of Overlapping Open Reading Frames Of Frog Virus 3, Type Species Of Ranavirus (Family Iridoviridae), Wendy Guat Hoon Tan

Dissertations

Frog virus 3 (FV3) is the type species member of the genus Ranavirus (family Iridoviridae ). To better understand the molecular mechanisms involved in the replication of FV3, including transcription of its highly methylated DNA genome, the complete nucleotide sequence of the FV3 genome has been determined. The FV3 genome is 105, 903 base pairs long excluding the terminal redundancy. The G+C content of FV3 genome is 55% and it encodes 98 non-overlappingpotential open reading frames (ORFs) containing 50 to 1293 amino acids. Eighty-four ORFs have significant homology to known proteins of other iridoviruses, whereas 14 are unique FV3 ORFs. …

Ionic Interaction Studies Of Bovine Copper, Zinc-Superoxide Dismutase, Hanan A. Hasan

Dissertations

No abstract provided.

Characterization Of Adaptive Changes In 5-Htia Receptor Systems Induced By Repeated Injections Of 5-Ht Uptake Inhibitors, Qian Li

Dissertations

No abstract provided.

Anaerobic Digestion And Acid Hydrolysis Of Nitrocellulose, Fong-Jung Tai

Dissertations

In this investigation, studies were conducted to evaluate the biodegradation of nitrocellulose in anaerobic batch reactors with and without the supplemental carbon inducers, such as cellulose, cellobiose, and lactose. Results from the anaerobic study show that degradation of nitrocellulose alone is difficult and that nitrocellulose degradative enzymes could be induced by the three inducers tested. As high as 48.91% conversion could be obtained at Cellulose/Nitrocellulose ratio of 1 to I. Studies also indicated that type 20 and 50 celluloses would be more effective and optimum pH was about 6.4 in biodegradation of nitrocellulose. Three testing systems, namely, single-stage, two-stage, and …

Na+-H+ Exchange And Na+-Li+ Exchange In Human Erythrocytes: An Nmr Investigation, Yuling Chi

Dissertations

No abstract provided.

Effects Of Dopamine And Dopaminergic Agents On Neuronal Activity Of The Ventral Pallidum/Substantia Innominata, Renata J. Maslowski-Cobuzzi

Dissertations

No abstract provided.

Depression As A Mediator Of Memory Biases In Alcoholics, Henry Jay Richards

Dissertations

No abstract provided.

The Evolving Biology Textbook In Chicago Secondary Schools: From The Progressive Era To The Present, Addie Beatrice Cain

Dissertations

No abstract provided.