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Preclinical Behavioral Assessments Of Mitraginyne, The Main Psychoactive Alkaloid In Kratom, Rachel Burroughs

Dissertations

Mitragyna speciosa (kratom) is a medicinal plant indigenous to Southeast Asia and Africa with a long history of use for a variety of ailments, including diarrhea, pain relief, cough suppression, and amelioration of opiate withdrawal symptoms. Despite its status as an herbal remedy, kratom is banned in some countries due to a myriad of negative health risks. Preclinical psychopharmacology studies of mitragynine (MG), the main alkaloid of kratom, indicate it has opioid-like effects at higher doses and psychostimulant-like effects at lower doses. The current study utilized three rodent behavioral paradigms to evaluate the putative psychostimulant effects of MG. The specific …

Prenatal Opioid Use And Neonatal Abstinence Syndrome: A Review Of The Neurophysiological, Neuropsychological, And Behavioral/Emotional/Social Impacts In The Pediatric Population, Candice Gore

Dissertations

The opioid epidemic over the past two decades has raised concerns regarding the developmental fetal impact of prenatal opioid use. Research in this area continues to grow, but largely has focused on treatment for neonates experiencing withdrawal symptoms postnatally. Long term clinical implications for this at-risk population have not been studied extensively leaving many gaps in research and highlighting the need for future empirical studies. This literature review will examine the neurophysiological, neuropsychological, and the behavioral/social/emotional impacts on infants, toddlers, and school aged children who were prenatally exposed to opioids with or without the diagnosis of neonatal abstinence syndrome. Providing …

Lifestyle Choices Related To Food Consumption And Their Relationship To Depression, Steven L. Jennings Jr.

Dissertations

Dieting, or the act of restricting oneself to small amounts or particular kinds of foods on the basis of health, spirituality, lifestyle, and moral decisions, is a practice that dates to 1066 A.D. However, with social media being as influential as it is, one might believe dieting is a new obsession or at least an obsession to which popular culture is returning. Despite this, it is only in recent years that researchers have begun to focus on the health benefits of such behaviors. The present study was designed to take this focus a step further by exploring the potential risks …

A Dialogical Approach To Peer Coaching For Asset Pedagogies, Elisabeth Van Beek

Dissertations

The purpose of this study was to understand how dialogical peer coaching using an asset pedagogical framework supported the development of culturally sustaining pedagogy (Gay, 2000; Ladson-Billings, 1994; Paris & Alim, 2017). This action research study followed one teacher and eight emergent bilingual students during an intervention. The intervention consisted of asset projects developed from the funds of identity framework (Esteban-Guitart, 2016). The peer coaching process consisted of a period of observation followed by repeated co-planning, coteaching, and reflection. The researcher took a dialogical stance on creating asset projects with the students as well as peer coaching with the teacher …

College Students’ Perceptions Of The Desert Canyons Of The Colorado Plateau, Jeremy Roger Deem

Dissertations

This study investigates eight college students’ perceptions of their experiences during an accredited outdoor education course entitled Technical Canyoneering. The course took place in four separate desert canyons of the Colorado Plateau, two in Arches National Park, and two on nearby Bureau of Land Management public space. Utilizing the data collection process of photo-elicitation, the participants’ time spent in the canyon settings was described in order to gain an understanding of their experiences, with particular attention paid to analyzing what perceptions those students ascribed to those experiences. The intent was threefold. First, the study makes understanding at how college students …

The Host Mir-17-92 Cluster Negatively Regulates Mmtv Replication By Targeting Its Genomic Rna Via Mir-92a, Jasmin Baby

Dissertations

The mouse mammary tumor virus (MMTV) is a milk-borne infectious agent capable of inducing mammary tumors primarily and to some extent, T-cell leukemias/lymphomas in mice. While MMTV has typically been employed to investigate fundamental processes in breast cancer initiation and progression, very little is known about the virus-host interplay during this process. MicroRNAs (miRNAs) are renowned master regulators of the transcriptome that influence key cellular activities, including anti-viral pathways that combat incoming viral infections. A recent study from our group revealed that unlike some retroviruses like bovine leukemia virus (BLV), MMTV does not encode virally-encoded miRNAs (v-miRs); rather, it perturbs …

Negative Memory Bias And The Impact Of Early Childhood Stress, Elizabeth Cecile Shmikler

Dissertations

Childhood Trauma is a pervasive problem in the United States, and research suggests it serves as a strong contributor and. Predictor for a multitude of negative mental and physical health disorders (Vrijsen et al., 2018). Negative memory bias, a neuropsychological precursor to later onset of adult mental health disorders, has been scientifically related to childhood trauma (Pollak et al., 1998). Despite numerous findings supporting the relationship between negative memory bias, childhood trauma, and mental health, little is known regarding the age of onset of negative memory bias (Brainerd & Reyna, 2012; Kujawa et al., 2011). Given the research supporting the …

Decision Making By The Prefrontal Cortex And The Role Of Dopamine In Pyramidal Neuron Function, Nesrien Muftah Milad Mohamed

Dissertations

There were two primary focuses of this research. The first aim was to investigate how students’ perception toward the flipped classroom and video learning correlate to their characteristics including their demographics, first generation status, English language learner status, Grit level, motivation types, quality of peer collaboration, and social self-efficacy. Our data indicated that there is significant correlation between student’s motivation status and attitude toward learning from video lectures. The intrinsically motivated students have a higher attitude toward learning from video. This study also demonstrates that participants with high Grit scores performed better than the participants with low Grit scores. The …

“I Don’T Believe The World Is Tilted”: Emotion, Struggle, And Relationship In Making Meaning With Texts, Alison Faye Oddie

Dissertations

The purpose of this study was to understand how the social and cultural practices of classroom literacy instruction afforded students opportunities to make meaning with texts. Research was conducted from a sociocultural perspective that focused on students as participants in social learning, in a context of interactive relations. This study was responsive to contemporary developments of the sociocultural tradition, recognizing the importance of emotion and other subjective means for constructing understanding. Two classes of a turnaround school, 18 Second-Grade students and 20 Third-Grade students, were observed as they participated in Interactive Read Aloud and Guided Reading over a period of …

Instance Segmentation-Based Depth Completion Using Sensor Fusion And Adaptive Clustering For Autonomous Vehicle Perception, Mohammad Z. El-Yabroudi

Dissertations

Depth sensing is critical for safe and accurate maneuvering in robotics and self-driving car (SDC) applications. Most recent LiDAR sensors, such as Ouster and Velodyne, offer 360 degrees of scanning at the rate of ten frames per second, making them very appropriate for autonomous driving applications. However, LiDAR point cloud data show many shortcomings, especially its data sparsity and unassigned nature, making it very challenging to utilize in applications such as perception, 3D object detection, 3D scene reconstruction, and simultaneous localization and mapping.

In this study, a novel framework using instance image segmentation and the raw LiDAR data for the …

Genetic And Environmental Drivers Of Microbial Colonization In Wild Birds, Sage Rohrer

Dissertations

Wild birds are teeming with microorganisms, ranging from commensal bacteria to eukaryotic parasites. These microbes impact host health in diverse ways; some long-term residents may aid digestion or provide immune system training, while others at times may induce disease. However, the factors driving the varying colonization patterns seen across taxa are still not fully understood, and wild avian populations are particularly understudied when compared to mammalian or domesticated systems. This work examines the relative importance of genetic and environmental factors driving microbiome composition and function in wild birds. We collected fecal and blood samples from Galapagos penguins (Spheniscus mendiculus …

Examination Of Badges To Increase Nursing Student Engagement: A Quasi-Experimental Study, Sharon C. Moritz

Dissertations

Finding ways to help nursing students understand the application of didactic content can be challenging for faculty. One challenge faculty face is motivating students with thought provoking and memorable content using traditional methods. Educational games and gaming attributes have been shown to affect student motivation and support learning. The aim of this study was to gain knowledge, filling a knowledge gap, regarding which gaming attributes motivate nursing students to become engaged in course content that is not graded. Badges were used as a reward system to examine if nursing students would complete an increased number of non-graded case studies, and …

Hiv And Early Life Stress On Neuroimaging And Risky Behavior, Paola Garcia Egan

Dissertations

This study examined the interactive effects of early life stress (ELS) and HIV on brain morphometry, diffusion-basis-spectrum-imaging (DBSI), risky decision-making, and sex-risk behavior. 122 people with HIV (PWH) and 113 people without HIV (PWoH), free of major psychiatric illness and neurological confounds, were stratified into high (≥ 3 events) vs. low (< 3 events) ELS [PWoH/low ELS (n = 57), PWoH/high ELS (n =56), PWH/low ELS (n = 43), PWH/high ELS (n = 79)] and underwent structural magnetic resonance imaging, DBSI, neuropsychological, and risky-behavior assessment; all PWH were virologically controlled. Compared to PWoH, PWH had smaller orbitofrontal cortex (OFC), parietal lobes, insula, caudate and anterior cingulate. No ELS effects were detected in volumetric measures. Significant interactions were found between HIV serostatus and ELS on the OFC and on cellularity of the inferior fronto-occipital fasciculus after multiple comparisons adjustment. Specifically, PWH/high ELS exhibited significantly smaller OFC and PWoH/high ELS show significantly larger OFC than the other groups. PWoH/high ELS exhibited higher DBSI cellularity (neuroinflammation proxy) of the inferior-occipital-fasciculus compared to PWoH/high ELS. Regardless of HIV status, executive function moderated the relationship between the OFC and sex-risk behavior such that individuals within the sample who performed above average on a measure of executive function and had a larger OFC reported fewer sex partners in past six months than individuals with smaller volumes. No interaction was found between HIV serostatus and ELS on risky behavior measures. Clustering analyses defined ELS subgroups in PWH that were determined by demographic characteristics, duration of infection, recent CD4+ T-cell count, nadir CD4+ T-cell count and high/low ELS.Even in PWH that are virologically controlled, without major current psychiatric comorbidities, there is evidence of a synergistic impact of ELS and HIV on OFC volumes. Higher volumes in the OFC were detrimental when associated with lower executive function scores or advantageous when associated with higher executive function. Findings suggest that ELS is associated with different brain signatures among PWoH and virally suppressed PWH. However, ELS was not directly associated with risky behaviors, and subgroups in PWH were characterized by demographic variables, past substance use and HIV clinical variables.

Where Are The Black Teachers? There Should Be More To Make It Even, Harolyn Harris

Dissertations

For years, Black teachers lived and worked in the communities that they served. I was one of those teachers who lived and worked in my community, but I noticed that there were not many of us. As a Black woman, mother and teacher, I was fully aware of the numbers of Black teachers declining. I wanted to know why.

In this study, I utilized my experience as a Black student, a Black teacher, and a Black administrator to reflect my feelings. I used Critical Race Theory as the theoretical lens on my stories using autoethnography as the research method. I …

Regulation Of Parkinson’S Disease-Associated Genes By Pumilio Proteins And Micrornas In The Human Neuroblastoma Cell Line Sh-Sy5y, Lisa J. Snoderly-Foster

Dissertations

Parkinson’s disease (PD) is the second most common age-related, neurodegenerative disease. A small collection of genes has been linked to PD, including SNCA encoding the protein alpha-synuclein that aggregates in Lewy bodies, a hallmark of PD. Overexpression of these genes can lead to pathogenesis, yet the regulatory mechanisms that control protein production of the genes are not fully understood. The SH-SY5Y neuronal cell line is a common model for the study of neurodegenerative diseases, including PD. However, SH-SY5Y cells are difficult to transfect, a process necessary for genetic manipulations and downstream analysis of RNA and protein. Herein, I describe the …

Cell Size Control Mechanisms In The Multiple Fission Cell Cycle Of Chlamydomonas, Dianyi Liu

Dissertations

Size homeostasis is achieved by a balance between cell growth and cell division, but the underlying mechanisms are yet fully understood. Green alga Chlamydomonas reinhardtii (Chlamydomonas) is a unique model for size control. Instead of the canonical binary fission cell cycle, Chlamydomonas uses a multiple fission cell cycle where a prolonged G1 phase is followed by rapid alternative n rounds of S/M (DNA synthesis and mitosis) cycles to produce 2ⁿ daughter cells. Two size checkpoints show size-dependence: the Commitment checkpoint governs a minimum size for a cell to divide at least once, and the S/M checkpoint governs the division …

Contextualizing The Emotive & Experiential Trajectories Of Arab Bilinguals In Higher Education: An Exploratory Sequential Mixed Method Study, Sabahet Bruncaj

Dissertations

Higher Education institutions in the UAE are increasingly adopting English as a Medium of Instruction. For native Arabic speakers, this educational practice has established a lingual impasse that relegates entry and serves as a signifier of ability. This study explores the experiential and emotional histories of conducting academic studying in a second language. The study is grounded in the theoretical framework of Emotions, whereby the exploration of affect and emotion-specifiers on bilinguals within the EMI in HE experiences and identified. The study applied an Exploratory Sequential Mixed method design led by Qualitative semi-structured interviews with Ph.D. students (n=11). This framework …

Learning By Design: The Transformative Pedagogical Experience Of College Students In Extending Monomodal Texts Into Multimodal, Najah Asaad Al Mohammedi

Dissertations

The architectonic shift in the semiotic, technology, and social environment paves the way to new perspectives of transformative pedagogies in multiliteracies and multimodal literacy for meaning-making through the configuration of gesture, space, image, sound, action, and various modes. Through a mixed-method explanatory sequential design, this study explores college students’ critical reading comprehension by changing a monomodal text into a multimodal one. In the redesigning process, a group of college students (n=4) were oriented to change a provided monomodal text into a multimodal text for the sake of understanding how the enactment of two different modes of a reading text contributes …

Acrylamide Mitigation By Novel Lactic Acid Bacteria And Bifidobacteria Isolated From Various Food Products: Optimization Of Mitigation, Effect Of Simulated Gastrointestinal Conditions, And Potential Mechanism, Amal Salem Albedwawi

Dissertations

Acrylamide is a toxic compound that is formed in heated carbohydrate-rich food. Scientists have analyzed acrylamide levels before, during, and after processing in order to study the mitigation strategies and, due to the lack of knowledge, this study aimed to: 1) screen 120 newly isolated LAB for their acrylamide removal; 2) optimize the conditions for 6 selected strains of pH, temperature, time and salt, using the Box-Behnken design (BBD), and analyze their acrylamide removal levels; 3) investigate acrylamide removal abilities of the selected LAB isolates under in-vitro digestion conditions, using the INFO-GEST 2.0 model; and 4) examine the mechanism of …

Impacts Of Intermediate And Long-Term Vitamin D Deficiency On The Murine Gastric Epithelial Cells And Acid Secretion, Shaima Raji Sirajudeen

Dissertations

The biological functions of vitamin D (VD) are diverse. It influences immunological response, cell proliferation and differentiation, and calcium homeostasis. However, over one billion people globally are VD deficient. The VD receptors, VDR and PDIA3, mediate the biological activities of VD. Studies on the biological roles of VD are numerous but very little on the stomach. The stomach is divided into three main parts – forestomach, corpus and antrum. Gastric glands, which make up the functional units of the stomach, are made up of numerous cell lineages and are located in the corpus and antrum, which are the glandular parts …

Investigations Of The Potential Use Of Macrophages In Gastric Stem Cell Activation/Differentiation And Ulcer Healing, Subi Sugathan

Dissertations

Stomach erosions and ulcerations are common health problems, and their therapy requires tissue regeneration. While current therapy for these disorders focuses on targeting parietal cells to inhibit acid secretion, the role of stem cells in the regeneration process is neglected. In this study, the aim is to investigate the potential use of macrophages to activate gastric stem cells in co-culture experiments and in a mouse model of gastric ulcer. Bone marrow-derived macrophages were prepared and cultured in the presence of lipopolysaccharides and interleukin-4 to, respectively, induce their differentiation into pro-inflammatory (M1) and anti-inflammatory (M2) macrophages. Polarization of these phenotypes was …

The Ecology And Evolution Of Species Rarity In Oaks (Quercus Spp.), Yingtong Wu

Dissertations

Rare species are susceptible to extinction due to ecological and genetic factors. Understanding the distribution, ecology, and evolution of rare species can provide useful information for effective conservation. To investigate species rarity, this dissertation focuses on a species-rich and ecologically diverse genus, Quercus (oaks). In Chapter 1, I aimed to understand how interactions between hosts and soil microbes contribute to habitat restriction in oak species. I performed a soil inoculum experiment on two pairs of sister oak species that show habitat divergence. I found that host-specific soil microbes contribute to habitat divergence and exclusion among sister species of oaks, but …

Synthesis Of Novel Dna-Binding Polyamides To Prevent Cancer-Related Gene Overexpression, Huy Q. Nguyen

Dissertations

Pyrrole-imidazole polyamides (PIPs) are nanomolecular compounds designed to fit in the tight space of DNA minor grooves. As analogs of Netropsin and Distamycin A, PIPs are specifically designed to recognize the base pairs of DNA sequences. PIPs have many biological applications, such as regulating gene expression, biochemistry pathway regulations, and suppressing the development of cancer cells. SETMAR gene is the chimeric fusion of the SET domain with the mariner transposase. Its protein (Metnase) has functions involving DNA repairs in the NHEJ pathway, regulating gene expression, DNA decatenation, etc. Despite not having an active transposable element, SETMAR still has an unknown …

“The Saloon Is Their Palace”: Race, Immigration, And Politics In The Woman’S Christian Temperance Union, 1874–1933, Ella Wagner

Dissertations

immigration, prohibition, race, suffrage, temperance, women's history

Burnout In The Field Of Forensic Psychology And Intervention Techniques, Taylor Gary

Dissertations

Forensic psychologists are at a heightened risk for encountering vicarious trauma, compassion fatigue, and burnout, all of which influence quality and continuity of care (Sansbury et al., 2015). The current literature review aims to discuss how burnout relates specifically to the field of forensic psychology. For the purposes of this discussion, clinical psychologists who perform forensic duties, as well as board certified forensic psychologists are considered. Research questions that are addressed in this discussion include what the existing research states regarding vicarious trauma in forensic settings, likely gender differences in burnout and vicarious trauma, and documented interventions that appear most …

A Past Not Present: Memory, Christianity, And Indian Removal Mission Sites In The Great Lakes And The South, Sean Thomas Jacobson

Dissertations

American Indians, cemeteries, Christianity, historic preservation, memory, public history

Evaluating The Impact Of Submarine Groundwater Discharge On Nutrients And Trace Elements In Coastal Systems: The Examples Of The Tuckean Swamp (Australia) And The Mississippi Sound (Usa), Amy Moody

Dissertations

Submarine groundwater discharge (SGD) is the advective flow of both fresh terrestrial groundwater and recirculating seawater through aquifer sediments, which is released into the coastal ocean. In this dissertation, I evaluated the impact of SGD on the distributions and input of trace metals and nutrients. In the Tuckean Swamp, an estuary in Australia dominated by coastal acid sulfate soils, I determined the impact of groundwater on Ba and U during the flood season, when the local aquifer is flushed out after a rapid increase in water table elevation. For Ba and U, groundwater contributed up to 18 and 66 % …

Sources And Distribution Of Microplastics In The United Arab Emirates, Rana Zeeshan Habib

Dissertations

Microplastics (plastic pieces size) are an emerging environmental contaminant that is ubiquitously present in terrestrial, aquatic and aerial environments. United Arab Emirates (UAE) is categorized among the highest domestic waste producing countries (on per capita basis) in the world. A large portion of waste ends up in the environment and contains macro-, meso- and microplastics. No comprehensive study has been carried out in the UAE to investigate the abundance, sources, distribution and impact of microplastics on its environment. Therefore, a comprehensive study to analyze the sources and distribution of microplastics in the UAE was urgently needed. The objective of the …

Exploring The Dynamics Of Primary Epsteinbarr Virus Infection Using A Novel Rabbit, Narendran Reguraman

Dissertations

Epstein-Barr virus (EBV) is a common herpesvirus asymptomatically carried by over 90% of the world population. However, in certain circumstances, the virus can lead to life-threatening diseases. Although the virus was isolated over 55 years ago, several fundamental aspects of the biology of EBV and the mechanism by which this virus induces pathology remain unknown. One major obstacle has been the lack of a suitable small animal model for EBV infection. We have recently shown that healthy rabbits are susceptible to EBV infection, and the virus establishes long-term latent infection typically seen in humans. The main objective of this dissertation …

Structural And Transient Kinetic Analysis On Mechanism-Based Inactivators Of Human Ornithine Aminotransferase, Arseniy Butrin

Dissertations

HCC, Hepatocellular carcinoma, MBI, Mechanism-based inactivator, OAT, Ornithine Aminotransferase