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Political Judgement, Freedom Of Thought, And Standardized Testing: A Critical Enquiry, Matthew Edward Schuler Jun 2012

Political Judgement, Freedom Of Thought, And Standardized Testing: A Critical Enquiry, Matthew Edward Schuler

Dissertations

The practice of “standardized” testing has been embedded in United States federal education policy since at least the passage of the Elementary and Secondary Education Act of 1965. First and foremost, for a test to be “standardized” the knowledge tested must be assumed to be “true”—universally accepted as being outside the bounds of values. This enquiry recognizes that the primary purpose of American public education has been to prepare students for the fundamental political office of citizen. Citizens must ideally be able to make informed political judgements utilizing the full spectrum of thought—knowledge in its broadest sense including knowledge unbounded …


Paleoclimatic Regimes Of The African Sahara Desert During Pleistocene And The Origin Of Groundwater In The Nubian Sandstone Aquifer System, Abdou A. Abouelmagd Jun 2012

Paleoclimatic Regimes Of The African Sahara Desert During Pleistocene And The Origin Of Groundwater In The Nubian Sandstone Aquifer System, Abdou A. Abouelmagd

Dissertations

This study involves an integrated three-fold approach to better understand the paleoclimatic regimes over the North African Sahara Desert with emphasis on the origin of groundwater in the Nubian Sandstone Aquifer System. Specifically, the nature and origin of the paleo-wind regimes that produced the precipitation that recharged the fossil aquifers in North Africa in the previous wet climatic periods are deciphered using inferences from stable isotope data, noble gases, remote sensing and GIS.

In the first phase, the progressive depletion trend of stable isotopic composition in the fossil groundwaters under influence of paleowesterlies across North African Sahara Desert is simulated …


Discrete Targeting Of Peptide And Non-Peptide Neurotransmitters By A Single Neuron, Daniel Kueh Jun 2012

Discrete Targeting Of Peptide And Non-Peptide Neurotransmitters By A Single Neuron, Daniel Kueh

Dissertations

The heart excitor (HE) motor neuron in the medicinal leech, Hirudo, releases acetylcholine (ACh) and a peptide, FMRFamide, to regulate the contractile activity of the heart tube and associated side vessels. Consistent with Dale’s Principle, it was assumed that both neurotransmitters were coreleased by all presynaptic varicosities. However, discrete peptide-positive and peptide-negative varicosities can be found consistently at particular sites of innervation on the heart tube. Dual-labeled HE neurons were produced by pressure injecting Neurobiotin into single HE neurons and applied antibodies against FMRFamide on the same preparations. Consistent with initial expectations, peptide-labeled varicosities were numerous and widely distributed …


Driver Behavior Classification And Lateral Control For Automobile Safety Systems, Jing Yang May 2012

Driver Behavior Classification And Lateral Control For Automobile Safety Systems, Jing Yang

Dissertations

Advanced driver assistance systems (ADAS) have been developed to help drivers maintain stability, improve road safety, and avoid potential collision. The data acquisition equipment that can be used to measure the state and parameter information of the vehicle may not be available for a standard passenger car due to economical and technical limitations. This work focuses on developing three technologies (longitudinal tire force estimation, driver behavior classification and lateral control) using low-cost sensors that can be utilized in ADAS.

For the longitudinal tire force estimation, a low cost 1Hz positioning global system (GPS) and a steering angle sensor are used …


Instabilities In Newtonian Films And Nematic Liquid Crystal Droplets, Te-Sheng Lin May 2012

Instabilities In Newtonian Films And Nematic Liquid Crystal Droplets, Te-Sheng Lin

Dissertations

The instabilities of Newtonian films and nematic liquid crystal droplets within the framework of the long wave (lubrication) approximation are studied. For Newtonian films, it is found that, under destabilizing gravitational force, a contact line, modeled by a commonly used precursor film model, leads to free surface instabilities without any additional natural or imposed perturbations. In addition, there is a coupling between the surface instabilities and the transverse (fingering) instabilities which leads to complex behavior. All the observed phenomena are characterized by a single parameter D = (3Ca)1/3 cot α where Ca is the capillary number and …


Virtual Reality Visual Feedback And Its Effect On Brain Excitability, Soha Saleh May 2012

Virtual Reality Visual Feedback And Its Effect On Brain Excitability, Soha Saleh

Dissertations

This dissertation examines manipulation of visual feedback in virtual reality (VR) to increase excitability of distinct neural networks in the sensorimotor cortex. The objective is to explore neural responses to visual feedback of motor activities performed in complex virtual environments during functional magnetic resonance imaging (fMRI), and to identify sensory manipulations that could further optimize VR rehabilitation of persons with hemiparesis. In addition, the effects of VR therapy on brain reorganization are investigated. An MRI-compatible VR system is used to provide subjects with online visual feedback of their hand movement. First, the author develops a protocol to analyze variability in …


Transport Of Terrigenous Sediment And Horseshoe Crab Eggs In The Swash Zone Of An Estuarine Foreshore In Delaware Bay, New Jersey, Usa, Sherestha Saini May 2012

Transport Of Terrigenous Sediment And Horseshoe Crab Eggs In The Swash Zone Of An Estuarine Foreshore In Delaware Bay, New Jersey, Usa, Sherestha Saini

Dissertations

Identification of processes responsible for egg exhumation and transport in the swash zone is paramount to conservation of species that use the intertidal foreshore. One example where the understanding of these processes is critical to egg exhumation, transport and deposition is in Delaware Bay, USA. Beaches in Delaware Bay provide foraging grounds to many shorebird species that migrate thousands of miles from Central and South America to feed on nutrient rich horseshoe crab eggs during their peak spawning season. Eggs laid at depth by horseshoe crabs are exhumed and transported by bioturbation, wave and swash processes and made available to …


Example Based Texture Synthesis And Quantification Of Texture Quality, Chandralekha De May 2012

Example Based Texture Synthesis And Quantification Of Texture Quality, Chandralekha De

Dissertations

Textures have been used effectively to create realistic environments for virtual worlds by reproducing the surface appearances. One of the widely-used methods for creating textures is the example based texture synthesis method. In this method of generating a texture of arbitrary size, an input image from the real world is provided. This input image is used for the basis of generating large textures. Various methods based on the underlying pattern of the image have been used to create these textures; however, the problem of finding an algorithm which provides a good output is still an open research issue. Moreover, the …


Image Statistical Frameworks For Digital Image Forensics, Patchara Sutthiwan May 2012

Image Statistical Frameworks For Digital Image Forensics, Patchara Sutthiwan

Dissertations

The advances of digital cameras, scanners, printers, image editing tools, smartphones, tablet personal computers as well as high-speed networks have made a digital image a conventional medium for visual information. Creation, duplication, distribution, or tampering of such a medium can be easily done, which calls for the necessity to be able to trace back the authenticity or history of the medium. Digital image forensics is an emerging research area that aims to resolve the imposed problem and has grown in popularity over the past decade. On the other hand, anti-forensics has emerged over the past few years as a relatively …


Upper Extremity Rehabilitation Using Interactive Virtual Environments, Qinyin Qiu May 2012

Upper Extremity Rehabilitation Using Interactive Virtual Environments, Qinyin Qiu

Dissertations

Stroke affects more than 700,000 people annually in the U.S. It is the leading cause of major disability. Recovery of upper extremity function remains particularly resistant to intervention, with 80% to 95% of persons demonstrating residual upper extremity impairments lasting beyond six months after the stroke. The NJIT Robot Assistive Virtual Rehabilitation (NJIT-RAVR) system has been developed to study optimal strategies for rehabilitation of arm and hand function. Several commercial available devices, such as HapticMaster™, Cyberglove™, trakSTAR™ and Cybergrasp™, have been integrated and 11 simulations were developed to allow users to interact with virtual environments. Visual interfaces used in these …


The Effect Of Photoperiod On Regulation Of Key Components Of The Life Cycle In The Bumble Bee Bombus Impatiens L. (Hymenoptera: Apidae)., Edgar Javier Hernandez May 2012

The Effect Of Photoperiod On Regulation Of Key Components Of The Life Cycle In The Bumble Bee Bombus Impatiens L. (Hymenoptera: Apidae)., Edgar Javier Hernandez

Dissertations

This study investigates how photoperiod affects internal characteristics of colonies of the temperate bumble bee Bombus impatiens particularly; development, reproduction, and social behavior. To answer this question I used an experimental approach using commercially obtained colonies of B. impatiens kept under controlled environmental conditions, and using the exposure to photoperiod as the only variable. Eight different photoperiod treatments were evaluated over the social phase of the colony’s life cycle. Colonies exposed to photoperiods that simulate the species natural temperate photoperiod exhibited larger growth rates, higher, oviposition rates, and higher brood survival than colonies exposed to constant photoperiods. Similarly, colonies exposed …


Host-Parasite Interactions In Galapagos Seabirds, Iris Ilena Levin May 2012

Host-Parasite Interactions In Galapagos Seabirds, Iris Ilena Levin

Dissertations

Parasites exhibit a wide range of life history strategies that contribute to different dispersal abilities, host specialization, transmission modes, life-cycle complexity and population structure. Understanding dispersal rates in hosts and parasites is instrumental in defining the scale at which coevolution may be occurring. In order to better understand how and when parasites move between different hosts, I studied a seabird – Hippoboscid fly ectoparasite (and vector) – Haemosporidian parasite system in the Galapagos Islands. I began by describing the Haemosporidian parasites of Galapagos seabirds, discovering a Plasmodium species parasite in Galapagos Penguins (Sphensicus mendiculus), and a new clade of Hippoboscid-vectored …


Evolution And Biogeography Of Fire-Eye Antbirds (Genus Pyriglena): Insights From Molecules And Songs, Marcos Maldonado Coelho May 2012

Evolution And Biogeography Of Fire-Eye Antbirds (Genus Pyriglena): Insights From Molecules And Songs, Marcos Maldonado Coelho

Dissertations

The importance of climatic and geologic factors as drivers of population differentiation and speciation in the Neotropical region has long been appreciated. However, many questions remain regarding their roles underlying the processes and patterns of diversification. Studies conducted in distinct regions containing a suite of geological and ecological conditions constitute ideal scenarios to assess the role of Pleistocene climatic changes, rivers, and mountain building as historical diversification mechanisms. In chapters 1 and 2, I used an integrative approach combining molecular phylogenetics, phylogeography and population genetics to elucidate the importance of climatic and geological factors as engines of diversification. I focused …


Genetic Variation In Potentially Virulent Vibrio Parahaemolyticus From The Northern Gulf Of Mexico, Nicholas Felix Noriea Iii May 2012

Genetic Variation In Potentially Virulent Vibrio Parahaemolyticus From The Northern Gulf Of Mexico, Nicholas Felix Noriea Iii

Dissertations

Vibrio parahaemolyticus (Vp) is a gram-negative bacterium found naturally in marine and estuarine environments. Vp is found in oysters including those which are later consumed by the public. Sub-populations of potentially virulent Vp contain specific virulence factors and are relevant human pathogens capable of causing gastroenteritis, wound infection, and death. The tdh and trh genes, both encoding hemolysins, have been correlated with the majority of clinical Vp isolates but have not been shown to be the definitive virulence factors.

A total of 146 Vp isolates from the northern Gulf of Mexico were collected and probed …


Effects Of Hypoxia And 4-Tert-Octylphenol On Gene Expression Profiles Of The Sheepshead Minnow (Cyprinodon Variegatus), Arthur Alan Karels May 2012

Effects Of Hypoxia And 4-Tert-Octylphenol On Gene Expression Profiles Of The Sheepshead Minnow (Cyprinodon Variegatus), Arthur Alan Karels

Dissertations

Hypoxia occurs in estuaries of northern Gulf of Mexico and world-wide, with increasing frequency/severity via eutrophication and anthropogenic influences. Hypoxia inducible factors (HIFs) form transcriptional complex and bind DNA at hypoxia responsive elements (HREs) in promoter regions of genes needed for systemic and cellular adaptation of fish to low dissolved oxygen (hypoxia, DO <2.0 mg/ml). Hypoxia-induced activation of HIF-αs can lead to a cascade of downstream activation, such as erythropoietin (EPO). Return to normal DO levels (normoxia), prolyl hydroxylases (PHDs) are activated to degrade HIF-αs back to baseline. Fish are affected by environmental estrogen mimics, like 4-tert-octylphenol (4tOP), binding estrogen receptor alpha (ERα) at estrogen responsive elements (EREs) and activating genes vitellogenin (VTG). Previous research showed overlap or crosstalk between these two mechanistic pathways. Hypoxia triggers unknown factors regulating ERE-mediated ERα signaling pathway, and stressor combinations could increase/decrease hypoxic or endocrine pathway. Research examined molecular/physiological effects of hypoxia (acute and chronic, moderate and severe) and 4tOP (~60μg/L)on adult male and/or female sheepshead minnow (Cyprinodon variegatus). Three genes identified, cloned, and sequenced (HIF-1α, HIF-2α, and PHD3), plus previously identified genes EPO and VTG, were examined in liver/testes exposed to hypoxia and/or 4tOP for cellular/physiological changes. Endpoints examined included mRNA expression from real-time PCR of HIF-1α, HIF-2α, PHD3, EPO, and VTG using cDNA from total RNA extracts, and microarray analyses of genes expressed during the transition from hypoxia back to normoxia. Phylogenetic analyses confirmed isolation of two HIF-α isofoms (HIF-1α and HIF-2α) and the PHD3 isoform. Significant up-regulation of PHD3 occurred within 10 hrs of chronic hypoxia, and persisted when severe (1.5 mg/L) and declined when moderate (~2.5mg/L). Significant up-regulation of HIF-1α and EPO occurred within 30 minutes to 2 hours of onset of acute severe and very severe (~1.08mg/L) hypoxia. Hypoxia acted similar to an estrogen mimic, with huge up-regulation of VTG gene expression in males, and increased VTG levels (additive effect) when hypoxia was combined with 4tOP. Microarray analyses showed 125 genes with significant transcriptional change, with up- or down-regulation from transitions of: (1) hypoxia (72 hrs) to normoxia (74 hrs) and (2) hypoxia+4tOP (72 hrs) to normoxia+4tOP (74 hrs).


Community College Students' Perceptions Of Effective Communication In Online Learning, Donna Alice Hill Parker May 2012

Community College Students' Perceptions Of Effective Communication In Online Learning, Donna Alice Hill Parker

Dissertations

This quantitative research project analyzed the application of instructional communication tools and techniques used by community college students to determine how they perceive communication in their online classes. Online students from a community college participated in this study by completing an electronic survey. Data analysis revealed that these participating students communicated primarily through written online communication tools such as posting of class notes, email, and discussions. Students lack exposure to more innovative communication tools such as teacher-made audio and video presentations or live conversations and demonstrations through the use of Wimba or other tools, which are readily available in the …


Personality Assessment In African Elephants (Loxodonta Africana), Kristina Marie Horback May 2012

Personality Assessment In African Elephants (Loxodonta Africana), Kristina Marie Horback

Dissertations

The following study assessed personality in twelve African elephants using both observational behavior coding and standardized trait rating methods, thus demonstrating consistent individual differences across time and contexts. During the summer of 2010 and 2011, over 640 hours of behavioral data were collected onsite at the San Diego Zoo Safari Park in Escondido, CA. Four coding-based personality traits were determined after analysis: PLAYFUL, CURIOUS, TOLERANT, and, AGGRESSIVE. This data was then compared to survey ratings completed by the animal keeper staff during both summers. Four rating-based personality traits resulted from this analysis: PLAYFUL, CURIOUS, TIMID, and, AGGRESSIVE. All eight composite …


Giving Students The Reins: Taking Advantage Of Service-Learning's Potential As A Pedagogy For Teaching Writing, Tereza Joy Kramer May 2012

Giving Students The Reins: Taking Advantage Of Service-Learning's Potential As A Pedagogy For Teaching Writing, Tereza Joy Kramer

Dissertations

Service-learning helps students experience the practical applications of learning to write well; it also offers opportunities for students to develop a sense of civic responsibility. Although service-learning is growing in popularity, this pedagogy is not prevalent in English departments. Additionally, service-learning courses across all disciplines typically do not empower students to make their own project decisions. Given these tendencies, it is useful to consider whether service-learning is an effective pedagogy for writing, whether students should be designing their own projects, and what writing instructors could do to facilitate students' growth as writers while completing projects in the community. This is …


I Still Have To Overcome Just Being Captured Inside Myself: The Experience And Meaning Of Peritraumatic Tonic Immobility Among Survivors Of Sexual Violence, Sunda Friedman Tebockhorst May 2012

I Still Have To Overcome Just Being Captured Inside Myself: The Experience And Meaning Of Peritraumatic Tonic Immobility Among Survivors Of Sexual Violence, Sunda Friedman Tebockhorst

Dissertations

While tonic immobility is a phenomenon well known and documented in the animal world, far less is known about its physiologic correlates and manifestation in human beings. This study examines the experience of tonic immobility from the perspective of seven women who have survived a sexual assault accompanied by tonic immobility. It yields a description of the experience of tonic immobility and how it is construed by survivors using phenomenological methodology. Results indicate that themes associated with the experience include a period of initial overwhelming confusion, feelings of terror, a desire to distance oneself from the experience, an intense desire …


Interactive Whiteboard Use: The Catalyst Of Student Achievement, Tenneille Terrell Lamberth May 2012

Interactive Whiteboard Use: The Catalyst Of Student Achievement, Tenneille Terrell Lamberth

Dissertations

The No Child Left Behind (NCLB) Act of 2001 forced school districts to become more accountable by requiring all students to read on grade level by the year 2014. However, President Obama’s educational policy shift is allowing states to develop their own accountability and improvement system. This study examined fourth and eighth grade math achievement on the Mississippi Curriculum Test, 2nd edition and interactive whiteboard use, attitudes and perceptions, and professional development. Data were collected from 21 participating Mississippi Gulf Coast area schools to determine if a correlation existed between fourth and eighth grade math achievement and interactive whiteboard use, …


Tripping With Stephen Gaskin: An Exploration Of A Hippy Adult Educator, Gabriel Patrick Morley May 2012

Tripping With Stephen Gaskin: An Exploration Of A Hippy Adult Educator, Gabriel Patrick Morley

Dissertations

For the last 40 years, Stephen Gaskin has been an adult educator on the fringe, working with tens of thousands of adults in the counterculture movement in pursuit of social change regarding marijuana legalization, women’s rights, environmental justice issues and beyond. Gaskin has written 11 books about his experiences teaching and learning with adults outside the mainstream, yet, he is virtually unknown in the field of adult education. He lists his religion as hippy; he is a member of the Counterculture Hall of Fame (inducted 2004), a convicted felon, a United States Marine, a Korean War combat veteran, and a …


The Effect Of Teachers’ Expectations And Perceptions On Student Achievement In Reading For Third And Fifth Grade Students, Alfreda Ragland Williams May 2012

The Effect Of Teachers’ Expectations And Perceptions On Student Achievement In Reading For Third And Fifth Grade Students, Alfreda Ragland Williams

Dissertations

All too often, a student’s lack of success is blamed on his or her background, and/or the parent or the parent’s educational level. Many factors such as socioeconomic conditions, student behaviors, attendance, and teacher demographics can directly or indirectly affect class environment, classroom management, interaction with students, and equal treatment of students. In addition, a teacher’s perception of students plays a vital role in the teacher’s expectations, interactions, and relationships with his or her students. The purpose of the study was twofold. First, this study investigated the relationship between teachers’ expectations of equal treatment of students, class environment, interaction with …


The Motivation Beliefs Inventory: Measuring Motivation Beliefs Using Four Motivation Theories, David C. Facer Jr. Phd May 2012

The Motivation Beliefs Inventory: Measuring Motivation Beliefs Using Four Motivation Theories, David C. Facer Jr. Phd

Dissertations

Among organizational consultants, human resources practitioners, and organizational leaders, there has been a resurgence of interest in the subject of employee motivation, in part due to the best-selling book, Drive (Pink, 2009). In this book, the author challenged readers to question their beliefs about what motivates employees; this challenge was based on research that questions the validity of widely used management approaches to employee motivation, particularly those based on reinforcement theory. Answering this challenge was difficult, however, given the lack of instruments designed to measure motivation beliefs at all, much less beliefs from a range of prevalent theories. Using principal …


Modern Approaches For Multifunctional Hydrolyzying Thiol-Ene Networks, Nicole Marie Mackey May 2012

Modern Approaches For Multifunctional Hydrolyzying Thiol-Ene Networks, Nicole Marie Mackey

Dissertations

Marine fouling has been an expensive and time-consuming process for navies and fishermen by the accumulation of marine algae and animals on artificial surfaces. Many coatings have been proposed to combat marine fouling in efforts to extend the lifetime of objects immersed in sea water. However, all coatings tested have either failed to resist fouling for a significant length of time or have released toxins into the environment. We have investigated an environmentally friendly approach to address the problem of marine fouling through the synthesis and preparation of a biocidal and a self-polishing coating. Both novel monomers were prepared containing …


The Relationship Between Academic Optimism And Academic Achievement In Middle Schools In Mississippi, Laquanta Murray Nelson May 2012

The Relationship Between Academic Optimism And Academic Achievement In Middle Schools In Mississippi, Laquanta Murray Nelson

Dissertations

As we constantly seek to increase educational attainment and increase student achievement in the United States, it is critical that we not only look at the effect of research based instructional practices or socioeconomic status on academic achievement, but also at any other factors that may potentially have a positive impact. The current state of education in Mississippi is still behind that of its counterparts, which suggests that providing schools with extra funds and an aligned curriculum alone will not raise student achievement.

According to Beard, Hoy, and Hoy (2009) academic optimism is a factor that influences academic achievement, even …


A Conductor’S Guide To The Poetic And Musical Style Of The Cante Jondo Based On The Work Of Mario Castelnuovo-Tedesco’S Romancero Gitano, Op.152, For Choir And Guitar With Selected Poems Of Federico Garcia Lorca’S Poema Del Cante Jondo, Elsa Patricia Ramierz-Hacker May 2012

A Conductor’S Guide To The Poetic And Musical Style Of The Cante Jondo Based On The Work Of Mario Castelnuovo-Tedesco’S Romancero Gitano, Op.152, For Choir And Guitar With Selected Poems Of Federico Garcia Lorca’S Poema Del Cante Jondo, Elsa Patricia Ramierz-Hacker

Dissertations

Composers around the world have found the poetry of Federico García Lorca a source of inspiration for solo and choral works. Mario Castelnuovo- Tedesco used seven texts from Lorca’s Poema del Cante Jondo (Deep Song Poems) in his work Romancero Gitano, op. 152, for choir and guitar.

The purpose of this study is to provide a conductor’s guide to the poetic, historical, and musical structure of the cante jondo. Cante jondo or “deep song” is considered the most serious and profound collection of songs in Flamenco music. These songs express the history and landscape of the Andalucían region. Donn Phoren …


Adhd In Young Boys: A Correlational Study Among Early Childhood Educators In Louisiana, Jessica Hart Stubbs May 2012

Adhd In Young Boys: A Correlational Study Among Early Childhood Educators In Louisiana, Jessica Hart Stubbs

Dissertations

Attention Deficit Hyperactivity Disorder is a psychiatric condition that has been increasingly diagnosed in young American children, with boys being diagnosed three times more frequently than their female peers. As a result, more children than ever are being treated with powerful stimulant medications which can have various desired and undesired effects. Early childhood curriculums have become more academic in nature, and early childhood teachers are under growing pressure to help their students master academic skills at earlier ages than ever before. Pharmaceutical companies aggressively market medications directly to consumers, promising improved academic and behavioral success for even the youngest children. …


The Relationship Between Emotional Intelligence And Leadership Effectiveness Among Sponsored Research Administrators, Ventez Derrell Jones May 2012

The Relationship Between Emotional Intelligence And Leadership Effectiveness Among Sponsored Research Administrators, Ventez Derrell Jones

Dissertations

The purpose of this study was to examine the relationship of emotional intelligence, as perceived by senior level university sponsored research administration professionals and their perceived leadership effectiveness, as measured by the Bar-On Emotional Quotient Inventory and the Kouzes and Posner Leadership Practices Inventory (LPI) for Self. Senior research administrators are now more than ever being faced with profusely, increasingly, difficult issues within the scope of their daily work processes.

The relevant review of literature focused on four key areas: theoretical rationale for examining emotional intelligence, the link between emotional intelligence and leadership effectiveness, effective leadership practices within education, and …


Joseph Kreines: A Biographical Sketch, An Analysis Of American Song Set For Band And A Descriptive Catalogue Of His Original Compositions And Transcriptions For Wind Band, Paul Eugene Weikle Jr. May 2012

Joseph Kreines: A Biographical Sketch, An Analysis Of American Song Set For Band And A Descriptive Catalogue Of His Original Compositions And Transcriptions For Wind Band, Paul Eugene Weikle Jr.

Dissertations

Joseph Kreines is a well-respected composer, conductor and educator who has maintained an illustrious musical career in Florida and elsewhere. Originally from Chicago, Illinois, Kreines came to Florida in the 1960s as the Associate Conductor of the Florida Symphony Orchestra. Other professional conducting appointments with the Brevard Symphony Orchestra, The Gulf Coast Symphony Orchestra and appointments as the conductor of the Florida Symphony Orchestra Youth Symphony, Pinellas Youth Symphony Orchestra and Brevard Youth Symphony Orchestra proved to be the launching of a successful free-lance career as guest conductor for hundreds of Secondary programs in Florida and elsewhere including band, orchestra …


Clam (Corbicula Fluminea) As A Potential Sentinel Of Human Norovirus Contamination In Freshwater, Xunyan Ye May 2012

Clam (Corbicula Fluminea) As A Potential Sentinel Of Human Norovirus Contamination In Freshwater, Xunyan Ye

Dissertations

The purpose of this study was to evaluate and validate the use of the clam Corbicula fluminea as a sentinel of human noroviruses (HuNoV) contamination in freshwater. The first specific aim was to develop a new method to extract HuNoV RNA from contaminated bivalves (e.g. oysters, clams) that would be much faster than existing methods. The procedure developed includes an initial total RNA extraction using TRI Reagent, followed by HuNoV RNA concentration and purification using biotinylated probe-capture technology. HuNoV RNA is finally detected by real-time RT-PCR. Using bivalve homogenates spiked with HuNoV, 100 PCR detection units of the virus was …