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Experimental Investigation On The Relationship Between Lead Exposure And Thyroids In Diabetes, Salah Sumar Ali Al-Zadjali

Dissertations

Lead exposure can cause multiple systemic toxicities, particularly affecting the hematopoietic, nervous and renal systems. However, its effects on the thyroid functions are not well elucidated and the published studies are controversial. In addition, although there are several experimental thyroid models, each one of them has its own limitations. Accordingly, in this dissertation, we investigated the possible relationship between lead exposure, thyroid functions and short-term systemic toxicity in two animal models, namely normal (non-diabetic) and diabetic animals. We also investigated the possibility of developing a hormonal thyroid model. In the non-diabetic model, Wistar rats were divided into five groups and …

Mechanisms Underlying Control Of Anti-Microbial Immunity By Acetylcholinesterase Inhibition, Ray M. Esam Al-Barazie

Dissertations

Inflammation is a crucial defense mechanism that protects the body from the devastating effects of invading pathogens. However, an unrestrained inflammatory reaction may result in systemic manifestations with dire consequences to the host. The extent of activation of the inflammatory response is tightly regulated through immunological and neural pathways. Previously, we demonstrated that cholinergic stimulation confers enhanced protection in experimental animals orally infected with a lethal dose of Salmonella typhymurium. In this study, we investigated the mechanism by which this enhanced protection takes place. We showed that cholinergic stimulation enhanced host survival following oral-route infection, which correlated with significantly reduced …

Experimental Investigation On The Relationship Between Lead Exposure And Thyroids In Diabetes., Salah Sumar Ali Al-Zadjali

Dissertations

Lead exposure can cause multiple systemic toxicities, particularly affecting the hematopoietic, nervous and renal systems. However, its effects on the thyroid functions are not well elucidated and the published studies are controversial. In addition, although there are several experimental thyroid models, each one of them has its own limitations. According, in this dissertation, we investigated the possible relationship between lead exposure, thyroid functions and short-term systemic toxicity in two animal models, namely normal (non-diabetic) and diabetic animals. We also investigated the possibility of developing a hormonal thyroid model.

In the non-diabetic model, Wistar rats were divided into five groups and …

Resonating Frequencies Of A Virtual Learning Community: An Ethnographic Case Study Of Online Faculty Development At Columbia College Chicago, David S. Noffs

Dissertations

Despite opportunities for artist educators to locate their practice in online learning environments, many remain reluctant to embrace virtual classrooms. As schools are being forced to create an increasing array of online courses, administrators are reluctant or unable to provide additional resources for equipping faculty with the skills needed to support online course development. Specific guidelines to train faculty how to teach online are often fragmented or local. Programs that demonstrate the ability to grow vibrant and connected online cultures may help guide future faculty development.

The Virtual Learning Community (VLC) Fellowship program at Columbia College Chicago supports informal, faculty …

Elucidation Of The Cellular And Molecular Mechanisms Of Missense Mutations Associated With Familial Exudative Vitreoretinopathy And Congenital Myasthenic Syndrome, Reham Mahmoud Mohammed Milhem

Dissertations

The endoplasmic reticulum (ER), within eukaryotic cells, is a hub for protein folding and assembly. Misfolded proteins and unassembled subunits of protein complexes are retained in the ER and degraded by a process termed endoplasmic reticulum associated degradation (ERAD). Frizzled class receptor 4 (FZD4) and muscle, skeletal, receptor tyrosine kinase (MuSK) are Wnt receptors. These proteins contain the frizzled cysteine-rich domain (Fz-CRD) required for dimerization in the ER. Mutations in FZD4 and MuSK genes are known to cause familial exudative vitreoretinopathy (FEVR, an autosomal dominant disease) and congenital myasthenic syndrome (CMS, an autosomal recessive disease), respectively. It was hypothesized that …

Synthetic Approaches To The Understanding Of Dna Nucleobase Methylation, Jagruti Rana

Dissertations

DNA methylation is a major source of genetic variation and cancer. Methylation occurs when nucleophilic DNA bases react with methylating agent methyl methanesulfonate (MMS), dimethyl sulfate (DMS), N-methyl-N-nitrosourea (MNU), N-methyl-N-nitro-N-nitrosoguanidine (MNNG), etc. N7-methyl-2'-deoxyguanosine (N7-methyl-dG, or ^7MedG) adduct is the most abundant DNA methylation products for most methylating agents. DNA polymerase actions on ^7MedG are difficult to study due to its instability against ring- opening hydrolysis and deglycosyl ati on. Oligonucleotides containing a single chemical adduct of ^7MedG cannot be chemically synthesized. In addition, ^7MedG is unstable in vivo …

Monitoring Of Cerebellar Injury Using Micro Ecog Signals In Ketamine/Xylazine Treated Rats, Gokhan Ordek

Dissertations

Much of the cerebellar research has been conducted in anesthetized animals, particularly using ketamine/xylazine combination in rats, and yet the absolute impact of the anesthesia on the neural circuit remains unanswered . In the current study, spontaneous electrical activity and sensory evoked potentials from the cerebellar surface with chronically implanted, flexible-substrate, multielectrode arrays in rats were collected and analyzed with the motor cortex signals. The power spectra and the intercontact coherence plots of the spontaneous activity in the awake-quiet animals extended up to 800 Hz in the cerebellum and only up to 200 Hz in the motor cortex. Ketamine/xylazine anesthesia …

E Pluribus Unum: What Individual Whales Can Tell Us About Enigmatic Species Distribution And Social Organization, Tanya Marie Lubansky

Dissertations

Large whales have historically been difficult to study and many aspects of their ecology remain unknown especially at the long -term population level. The ability to identify individual whales based on natural markings provides the opportunity to track individuals over time and space; this data may offer more insight into the ecology of whales than previously imagined. This study demonstrates use of photo-identification data to model both social structure and habitat selection, minimizing the need for invasive research and greatly increasing the sample size used in such endeavors. A conditional logistic model is written for a 20-year sightings dataset on …

Developing An Insulin Producing Tissue Using Mouse Embryonic Stem Cells, Neha Mahendrakumar Jain

Dissertations

This thesis describes derivation of pancreatic insulin producing cells (IPCs) from mouse embryonic stem cells and development of three-dimensional (3D) engineered tissue system to provide physiologic culture conditions for IPCs. Upon using a previously established protocol, IPCs have been successfully derived from mouse embryonic stem cells and characterized in vitro . IPCs not only express classical markers of pancreatic beta cells but also exhibit glucose responsive behavior. Interestingly while deriving IPCs from mouse embryonic stem cells, islet endothelial cells have also been identified and successfully isolated from the culture. Derivation of a pure population of endothelial cells expressing specific markers …

Galapagos Seabirds' Lice Community: Host Hetero-Specific Interactions And Parasite Evolution, Jose Luis Rivera

Dissertations

My dissertation focused on understanding the factors behind host-parasite specificity and parasite diversification, using Galapagos seabirds and their ectoparasites as the study system. This system comprised the seabird hosts (magnificent and great frigatebirds, Nazca, blue-footed and red-footed boobies) and nine species of ectoparasitic lice (one Pectinopygus ischnoceran louse species infecting each host, two species of Colpocephalum amblyceran lice, one on each frigatebird species, and two shared amblyceran lice, Eidmanniella albescens found on Nazca and blue-footed boobies and Fregatiella aurifasciata found on the two frigatebirds). Using as focal species Eidmanniella albescens and Fregatiella aurifasciata, which infect multiple hosts, I analyzed how …

Design, Synthesis, And Biological Evaluation Of Folate Targeted Photosensitizers For Photodynamic Therapy, Laura Jean Donahue

Dissertations

Photodynamic therapy (PDT) is used to treat cancer, and involves a highly conjugated molecule, called a photosensitizer (PS), which is excited by wavelengths of light from visible to infrared. Photosensitizers that are highly conjugated will absorb longer wavelengths (600 – 900 nm), and have the potential to destroy cells of deeper tissue cancers. In PDT, a PS is administered to the patient, and after an appropriate time delay, the tissue is then exposed to light of a specific wavelength necessary for excitation of the PS. A transfer of energy can take place between the excited PS and oxygen that is …

Influence Of Maternal Prior Life Adversity On The Psycho-Neuroendocrine-Immune Profile During Pregnancy, Karen J. Kotz

Dissertations

Pregnancy is accompanied by a multitude of physical and psychological changes. Adaptation to these changes through reduced anxiety and attenuated stress responsiveness is necessary across gestation and into the postpartum period for optimal maternal-infant health. In contrast, exposure to higher amounts of stressors during pregnancy can disrupt neuroendocrine-immune processes required for successful pregnancy outcomes. Evolving evidence demonstrates that exposure to adversity early in life has long-lasting effects on stress response systems that alter stress reactivity during adulthood. Given this evidence, it is posited that women who experience greater pre-pregnancy adversity during their childhood are at greater risk for negative maternal-infant …

The Global Dystopian: Twenty-First Century Globalization, Terrorism, And Urban Destruction, Ryan Frank Peters

Dissertations

The new millennium has witnessed a staggering outpouring of literature, film and art which depict dystopian and post-apocalyptic societies. These stories have come in the form of massively popular films like the Matrix and Hunger Games trilogies; Emmy-winning television adaptations such as The Walking Dead; and Pulitzer Prize-winning novels like Cormac McCarthy’s The Road, or Kazuo Ishiguro’s Booker Prize-nominated Never Let Me Go. In the last five years, a wave of critically-lauded video games – The Last of Us, Bioshock Infinite, and Dishonored come immediately to mind—have depicted dystopian worlds while reaching mass audiences. Combined, The Last of Us and …

The Role Of Alveolar Macrophages In Pulmonary Inflammation After Intoxication And Burn Injury, Jill Ann Shults

Dissertations

A positive blood alcohol concentration is detected in nearly half of burn patients admitted to the emergency room. The combined insult of being intoxicated at the time of burn injury results in more clinical complications, in comparison to non-intoxicated burn patients. Severe burn, with or without inhalation injury, is a common predisposing factor for the development of acute respiratory distress syndrome (ARDS). Exacerbated pulmonary inflammation and a net result of insufficient gas exchange underlie the large percentage of burn fatalities due to pulmonary complications. Previous studies in our laboratory indicate a drastic elevation in pulmonary inflammation in a mouse model …

Mechanisms Of Estrogen Receptor Alternative Splicing And The Consequences For Aging In The Female Brain, Cody Lee Shults

Dissertations

The advances in healthcare and scientific knowledge have resulted in longer life expectancies in women. These advanced ages in women now means that they are experiencing the effects of age-related changes in the body for much longer periods of time, mainly reproductive senescence, resulting in the loss of circulating ovarian hormones. The age at which menopause occurs has not changed, resulting in women now living over a third of their lives in a postmenopausal state.

The major circulating estrogen produced by the ovaries, 17β-estradiol (E2), has many homeostatic effects in the body like neuroprotection and cardioprotection. Hormone replacement therapy (HT) …

Effects Of Aggregated Alpha-Synuclein On Cultured Neuronal Cells, David Freeman

Dissertations

α-synuclein dysregulation is a critical aspect of Parkinson's disease pathology. Recent studies have observed that α-synuclein aggregates are cytotoxic to cells in culture and that this toxicity can be spread between cells. However, the molecular mechanisms governing this cytotoxicity and spread are poorly characterized. Recent studies of viruses and bacteria, which achieve their cytoplasmic entry by rupturing intracellular vesicles, have utilized the redistribution of galectin proteins as a tool to measure vesicle rupture by these organisms. Using this approach, we demonstrate that α-synuclein aggregates can induce the rupture of lysosomes following their endocytosis in neuronal cell lines. This rupture can …

Signaling Between Two Sensor Kinases Controls Biofilms And Host Colonization In A Bacterial Symbiont, Allison N. Norsworthy

Dissertations

Organisms within all domains of life must acclimate to fluctuating environments to survive. To do this, cells utilize sensory circuits, which function to connect environmental stimuli to an intracellular response. One common sensory pathway utilized by bacteria is two-component signaling (TCS), composed of an environmental sensor (the sensor kinase, SK) and a cognate, intracellular effector (the response regulator, RR). The marine bacterium Vibrio fischeri uses an elaborate TCS phosphorelay containing a hybrid SK, RscS, and two RRs, SypE and SypG, to colonize its natural squid host, Euprymna scolopes. This TCS pathway regulates V. fischeri's ability to form a biofilm, or …

Extended Treatment Window Exists For Improving Sensorimotor Recovery Using Anti-Nogo A Immunotherapy In Adult Rats With Chronic Stroke Deficits, Katherine Marie Podraza

Dissertations

Stroke related death ranks as the fourth most common cause of mortality in the United States. Current therapeutic options following stroke are limited. One potential therapy involves the use of anti-Nogo-A immunotherapy post-stroke. Our laboratory has shown that the administration of this novel treatment results in sensorimotor recovery in adult and aged rats. This therapy has been shown to be efficacious when applied immediately, 24 hours, and 1 week post-stroke in rats with mild to moderate sensorimotor deficits. In addition, recent results suggest that administration of anti-Nogo-A immunotherapy up to 9 weeks after stroke still induces functional recovery and neuroanatomical …

The Representation Of God In First Corinthians 8-10: Understanding Paul In The Context Of Wisdom, Philo, And Josephus, Trent Alan Rogers

Dissertations

The interpretation of 1 Cor 8-10 is complicated by several factors. Scholars have noted the apparent contradictions in the text (primarily an issue within ch. 8) and also the remarkable changes in Paul's tone (primarily an issue with how 10:1-22 relates to 8:1-13 and 10:23-11:1). I argue that Paul consistently prohibits Christians from eating food sacrificed to idols; by appealing first to their obligation to love other believers and then to their obligation of exclusive faithfulness to Christ. What has largely been overlooked is the way that these arguments are made on the basis on one's understanding of God and …

The Gut-Liver Axis Regulates Pulmonary Inflammation After Intoxication And Burn Injury, Michael M. Chen

Dissertations

The increasing prevalence of binge drinking and its high association with traumatic injury warrant further investigation into the mechanisms underlying the worsened clinical outcomes associated with this combined injury. Existing literature suggests the liver is at the center of the postburn systemic response with crosstalk between the intestinal microbiome and the liver playing an important role after injury. There is a paucity in the literature, however, regarding the role of the gut-liver axis in the common clinical scenario of burns preceded by alcohol intoxication. Previous studies in our laboratory demonstrated intoxication potentiates postburn hepatic damage and IL-6 production in a …

Negotiating Diabetes: Professional Diabetes Care Work In The U.S., Melissa Marie Gesbeck

Dissertations

The rising incidence of diabetes mellitus (types 1 and 2) transcends national borders, creating a global pandemic. The U.S. leads the world in diabetes prevalence, despite its wealth and access to sophisticated medical technologies. Within health care, there is an increasing focus on lifestyle and behavioral interventions that research suggests may be the key to reversing this trend, but thus far their effectiveness in practice is uneven at best. In order to gain a better understanding of the other factors involved in professional diabetes care work that might support or inhibit effective diabetes management, this research treats diabetes as a …

Research And Reality: Towards Responsible Medical Research For Catholic Universities, Michael Patrick Mccarthy

Dissertations

It is frequently noted that 90% of medical research stands to benefit only 10% of the population. While others dispute the numbers, there is little doubt that disparities exist in the global agenda for medical research. One needs no clearer example of research—not to mention public health and health care delivery—disparities than the Ebola outbreak that plagues West Africa. Despite these disparities, Catholic universities continue to engage in the social practice of medical research that deviates very little from the national funding priorities established through the National Institute for Health.

This dissertation argues that these unjust disparities are perpetuated by …

Characterization Of The Effect Of Anti-Nogo-A Antibody Treatment On Sensorimotor Recovery After Traumatic Brain Injury, Stephen Nawara

Dissertations

Estimates indicate that over 5 million Americans are currently living with Traumatic Brain Injury (TBI) related disability. Much effort has been put into enhancing functional recovery with physical therapy and other rehabilitative approaches. These strategies are thought to function by facilitating the brain’s innate capacity for neuroregeneration and neuroplasticity. However, the success of these strategies may be limited due the presence of growth- and plasticity- inhibiting factors expressed in the adult brain.

The presence of the membrane protein Nogo-A has been associated with reduced neurite outgrowth in vitro, identifying it as one such molecule. In addition, rodents treated with antibodies …

Gender-Homogenous Mentoring, Spiritual Wellbeing, And Self-Efficacy Beliefs In African American Male Adolescents: A Test Of Three Models, Toussaint David Whetstone

Dissertations

Many African American male adolescents mature without the influence of an adequate social model, or a positive, same sex (or gender-homogenous) mentor. Thus, it may be difficult for African American male adolescents to reach adulthood having developed the perceived capability to be successful within specific domains that American society commonly associates with a healthy life course trajectory. A large body of research has suggested that vicarious experience or role modeling is a primary source of efficacy information in a variety of life domains. Research has also suggested that modeling effects are enhanced if the subject and model are similar, especially …

Adaptive Control For Autonomous Navigation Of Mobile Robots Considering Time Delay And Uncertainty, Stephen Kofi Armah

Dissertations

Autonomous control of mobile robots has attracted considerable attention of researchers in the areas of robotics and autonomous systems during the past decades. One of the goals in the field of mobile robotics is development of platforms that robustly operate in given, partially unknown, or unpredictable environments and offer desired services to humans. Autonomous mobile robots need to be equipped with effective, robust and/or adaptive, navigation control systems. In spite of enormous reported work on autonomous navigation control systems for mobile robots, achieving the goal above is still an open problem. Robustness and reliability of the controlled system can always …

Herbert Spencer And His American Audience, Joel F. Yoder

Dissertations

The philosopher Herbert Spencer (1820-1903) is little remembered today, but in the late nineteenth century he was a world-renowned figure and widely read. Spencer was popular in his native England, but even more highly regarded in America. Modern scholars generally understand this popularity as stemming from Spencer’s social Darwinism—that is, his belief that natural selection does and should operate on humans to improve mankind. On the other hand, many of those who have studied Spencer’s work claim that he was not a social Darwinist at all. It is my contention that Spencer was a social Darwinist, but that other aspects …

A Biblical And Missiological Framework For Cross-Cultural Mission: A Case Study Of The Lobi Funeral Rites In Burkina Faso, Boubakar Sanou

Dissertations

Problem

Converts to Christianity in traditional contexts often face pressure to continue traditional rituals and practices which sometimes contain unbiblical elements. Non-selective performance of traditional practices can produce dual allegiance and syncretism. Such is the case with Lobi Seventh-day Adventists concerning their traditional funeral rites of passage. Some core elements of these traditional funeral rites, in which all community members are expected to fully participate, conflict with biblical teachings on the human condition after death.

Method

This dissertation starts by laying the theoretical and theological basis for addressing the problem. The dissertation then uses ethnographic research of funeral rites among …

Computational Investigation Of The Influence Of Heating Modes And Moisture Content On Pyrolysis And Ignition Of Live Fuels, Bangalore Lingaraj Yashwanth

Dissertations

The burning of an isolated leaf-like element was computationally investigated in a series of studies, motivated by recent burning experiments performed on live leaves of manzanita (Arctostaphylos glandulosa). In this study, the relative impor- tance of heating modes, effect of fuel moisture content on pyrolysis and combustion of live fuels is explored in stages. A preliminary study was conducted on a simpli- fied one-dimensional configuration, using Gpyro. The heating sources were modeled through convection and/or radiation as boundary conditions. Results showed that the increase in radiative source temperature substantially affects ignition time; how- ever, it has marginal influence on mass …

Development Of Gc-Ms Database Of Essential Oil Components By The Analysis Of Natural Essential Oils And Synthetic Compounds And Discovery Of Biologically Active Novel Chemotypes In Essential Oils, Prabodh Satyal

Dissertations

Based on the numerous side effects of synthetically-derived medicines, many people are trying to transform their lifestyles through naturopathy. Unfortunately, due to a lack of complete instrumental techniques and strict government regulation, adulteration of natural products is rampant all over the world. About 80% of commercially available, so-called “natural” essential oils are adulterated for economic profit. So to track adulteration, the Sat-Set Gas Chromatography-Linear Retention Index-Mass Spectrometry (GC-LRI-MS) library has been created. This extensive library contains 2,700 pure, complete mass spectra, including retention index, molecular weight, and formula information. Those mass spectra were collected from 590 different essential oil samples …

Characterization Of The Afternoon To Evening Transition Of The Planetary Boundary Layer Over North Alabama And Implications For Convective Maintenance, Enhancement, And Initiation, Stephanie Mullins Wingo

Dissertations

Investigation of 143 cases of the north Alabama planetary boundary layer's afternoon to evening transition (AET) using surface, profiling, and radar observations contributes to the relative scarcity of datasets for this period. Results indicate several characteristics previously deemed important for convective initiation and/or maintenance, namely a rise in boundary layer water vapor and convergence. Waning surface heating causes turbulent eddies to decay, decreasing vertical heat and momentum fluxes, evident in declining surface wind speeds as horizontal flow above the surface layer increases. After surface temperature variances decline, the rate at which vertical velocity fluctuations decay increases as vigorous thermals diminish, …