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Functional Characterization Of Rice Malate Synthase In Arabidopsis Responding To Salt Stress, Supisara Thanabut
Functional Characterization Of Rice Malate Synthase In Arabidopsis Responding To Salt Stress, Supisara Thanabut
Chulalongkorn University Theses and Dissertations (Chula ETD)
Salt stress is one of the major abiotic stresses that seriously limit plant growth and development and can lead to a significant reduction in crop yield, especially rice. Salt stress affects plants via ionic toxicity and osmotic stress. Osmotic stress results in a reduction of water absorption ability. Ionic toxicity causes Na+ toxicity, which disrupts photosynthesis, protein synthesis, and enzyme activity. The previous study of KDML105 rice transcriptome profile and qRT-PCR revealed that rice malate synthase (OsMS) encoding malate synthase, which is one of the key enzymes in glyoxylate cycle was up-regulated under salt stress, so OsMS may involve in …
Development Of A Device For Postural Change While Sitting For The Prevention Of Neck Or Low Back Pain In Office Workers, Nipaporn Akkarakittichoke
Development Of A Device For Postural Change While Sitting For The Prevention Of Neck Or Low Back Pain In Office Workers, Nipaporn Akkarakittichoke
Chulalongkorn University Theses and Dissertations (Chula ETD)
The objective of this thesis was to develop a device for postural change while sitting for the prevention of neck or low back pain in office workers. This thesis was divided into four stages: 1) to evaluate the effects of postural shifting frequency on perceived musculoskeletal discomfort during 1 hour of sitting in healthy office workers; 2) to evaluate the effects of the postural shift intervention on the 12-month incidence of the onset of neck and low back pain in high-risk office workers; 3) to investigate the efficacy of postural shift interventions on recovery duration and recurrence of neck and …
Epidemiology, Molecular Genetic, And Evolutionary Analysis Of Humanrotavirus A Infection In Thailand, Fajar Budi Lestari
Epidemiology, Molecular Genetic, And Evolutionary Analysis Of Humanrotavirus A Infection In Thailand, Fajar Budi Lestari
Chulalongkorn University Theses and Dissertations (Chula ETD)
Rotavirus A (RVA) infection remains one of the major causes of viral diarrhea in young children worldwide. Despite the success of RVA vaccines including RotaTeq in significantly reducing morbidity and disease severity associated with hospitalization, national immunization against RVA have only just begun in Thailand in 2020. Consequently, possible RV vaccine shedding among pediatric vaccine recipients has not been rigorously documented here.The first part of my study was summarize the virology, disease burden, prevalence, distribution of genotypes and seasonality of RVs, and the current status of RV vaccination in Southeast Asia (Cambodia, Indonesia, Lao People’s Democratic Republic, Malaysia, Myanmar, Philippines, …
Identification Of Α-Glucosidase Inhibitors From Black Garlic, Pattraporn Tantiwatcharachai
Identification Of Α-Glucosidase Inhibitors From Black Garlic, Pattraporn Tantiwatcharachai
Chulalongkorn University Theses and Dissertations (Chula ETD)
Black garlic has emerged as one of the fastest-growing health food products on the market. It is processed by incubating fresh garlic under high temperature and high humidity conditions. The process causes the changes in characteristics of black garlic such as texture, flavor, nutrient contents, chemical composition and it pharmacological properties, especially antidiabetic activity. In the present investigation, we applied α-glucosidase inhibition guided isolation to identify active compounds. Four compounds named 5-hydroxymethylfurfural (5-HMF), 5-hydroxymethyl-2-furancarboxylic acid (HMFCA), 4-(hydroxymethyl)phenol and 2-deoxy-ribono-1,4 lactone were isolated. On evaluation against α-glucosidase, HMFCA, 4-(hydroxymethyl)phenol, and 2-deoxy-ribono-1,4 lactone were active against maltase with IC50 values of 18.83 …
Chemical Constituents Of Fan-Si Abutilon Indicum Stems, Arum Restu Widyasti
Chemical Constituents Of Fan-Si Abutilon Indicum Stems, Arum Restu Widyasti
Chulalongkorn University Theses and Dissertations (Chula ETD)
This study aimed to isolate secondary metabolites from the stems of Abutilon indicum and to evaluate their α-glucosidase, pancreatic lipase, α-chymotrypsin inhibitory activity. Chromatographic fractionation of the n-hexane crude extracts led to the isolation of three known metabolite (1-3). The crude extract from all parts of the plant were evaluated for inhibitory activity on the alpha-glucosidase enzyme. The DCM-soluble roots were active with 10.2% at 25 μg/mL. The hexane and MeOH-soluble stems were active with 11.0% at 25 μg/mL and 8.0% at 0.25 μg/mL. The hexane, DCM, and MeOH-soluble leaves are active with 20.2% at 0.025 μg/mL; 20.0% at 0.025 …
Thin-Layer Chromatography For Determination Of Phenolic Compound Profile And Antioxidant Activity Of Thai Honey, Pattraporn Chobpradit
Thin-Layer Chromatography For Determination Of Phenolic Compound Profile And Antioxidant Activity Of Thai Honey, Pattraporn Chobpradit
Chulalongkorn University Theses and Dissertations (Chula ETD)
In this study, a small-scale method using thin-layer chromatography (TLC) was developed to determine profile and antioxidant activity value of Thai honey samples obtained from different flora sources. Prior to TLC analysis, the samples were prepared by solvent extraction with dichloromethane. Using 2, 2-diphenyl-1-picrylhydrazy radical (DPPH•) reacted with the samples extract prior to TLC spot and color detection with imageJ, half-maximal inhibitory concentration (IC50) values of seven honey samples was obtained in a range of 9 to 22 mg/mL in comparison with IC50 values of 0.13 mg/mL for L-ascorbic acid (LA), along with limit of detection (LOD) and limit of …
Discrimination Of Weedy Rice By Using Near-Infrared Spectroscopy Combined With Chemometrics, Sureerat Makmuang
Discrimination Of Weedy Rice By Using Near-Infrared Spectroscopy Combined With Chemometrics, Sureerat Makmuang
Chulalongkorn University Theses and Dissertations (Chula ETD)
Weedy rice is one of the most notorious weeds occurring in rice-growing areas, especially in South-East Asia. Weedy rice especially in form of paddy seed is difficult to manage and separate as they provide common features (morphological resemblance) to cultivated rice. This work presents a modification of self-organizing map (SOMs) for the classification of weedy rice from cultivated rice via in situ direct sample analysis from paddy seed using near-infrared (NIR) spectroscopy and hyperspectral NIR camera. The sample pretreatment was carried out by a cyclone vacuum machine to remove the contaminated particles and other impurities. The physical characteristics and the …
Developing A System Of Community-Based Agritourism For Sustainable Local Food Systems: A Multi-Case Study Of Rural Livelihood Diversification And Sustainable Urban Consumption In City-Regional Bangkok, Sofia Anna Enrica Cavalleri
Developing A System Of Community-Based Agritourism For Sustainable Local Food Systems: A Multi-Case Study Of Rural Livelihood Diversification And Sustainable Urban Consumption In City-Regional Bangkok, Sofia Anna Enrica Cavalleri
Chulalongkorn University Theses and Dissertations (Chula ETD)
Current food systems fail to directly link rural producers and urban consumers. This research explores and categorizes emerging community-based agritourism practices as strategies to reconnect rural food producers with urban consumers. The main research question of this study is: how can community-based agritourism link rural food producers and urban consumers as a rural livelihood diversification strategy? Mixed methods for data collection were selected to answer this question and analyzed with a deductive and inductive approach. These include the review of secondary grey and academic literature, shadow observation in three rural provinces, content validity index calculation performed by experts (n = …
Solid-State Modification Of Tapioca Starch Using Non-Thermal Plasma, Natchanon Srangsomjit
Solid-State Modification Of Tapioca Starch Using Non-Thermal Plasma, Natchanon Srangsomjit
Chulalongkorn University Theses and Dissertations (Chula ETD)
The objective of this research was to investigate the physical, chemical, and functional properties of tapioca starch subjected to solid-state modification by using nonthermal dielectric barrier discharge (DBD) plasma treatment using different gases (helium and argon), voltage levels (10 kV and 15 kV), and treatment times (5, 10, and 15 minutes). After treatment, an increase in L* value and a marginal decrease in pH value of all treated samples were noted. Scanning electron micrographs showed dented starch granules after the plasma treatment but showed birefringence. From XRD measurement, all samples showed a characteristic C-type diffraction pattern but a reduction in …
The Effectiveness Of Asiaticoside On Osteogenic Differentiation Ability In Aging Human Periodontal Ligament Cells In Vitro, Adisa Suthirathikul
The Effectiveness Of Asiaticoside On Osteogenic Differentiation Ability In Aging Human Periodontal Ligament Cells In Vitro, Adisa Suthirathikul
Chulalongkorn University Theses and Dissertations (Chula ETD)
Retaining teeth is essential for the quality of life of the aging population. However, aging has biological effects in periodontal ligament cells with significant changes in their proliferative rate and regenerative function. Asiaticoside, extracted from Centella asiatica, was found to have potential in osteogenic differentiation and mineralization in human periodontal ligament cells (HPDLCs). The aim of this study is to investigate the effect of asiaticoside on the aging HPDLCs osteogenic differentiation and mineralization. Aging HPDLCs, retrieved from patients age 60 and above, were confirmed aging status using Senescence-Associated ß -Galactosidase assay. The cells were incubated with various concentrations of asiaticoside …
Changes In Surface Water And Sediment Quality At A Complex Tidal-Influenced River: A Case Study Of Ganh Hao River, Mekong Delta, Vietnam, Muoi Le Van
Chulalongkorn University Theses and Dissertations (Chula ETD)
The research was carried out to determine the extent of surface water and sediment pollution using the physicochemical parameters and heavy metal concentrations. The surface water and sediment samples were obtained from 25 sites along the river on March 22 and September 5, 2019 at the main river influenced by the complex tidal regime and aquaculture practices in Ca Mau peninsular in the Vietnamese Mekong Delta. The water quality was compared with international and national standards, while the sediment quality was compared with Vietnamese standards and pollution indices (geo-accumulation index, contamination factor, and pollution load index). Principal component analysis (PCA) …
Health Risk Assessment Of Toxic Metals In Commonly Consumed Salad Leafy Vegetables In Bangkok, Zin Htoo Hlyan
Health Risk Assessment Of Toxic Metals In Commonly Consumed Salad Leafy Vegetables In Bangkok, Zin Htoo Hlyan
Chulalongkorn University Theses and Dissertations (Chula ETD)
The objectives of this study were 1) to investigate concentrations of toxic metals including As, Cd, and Pb in the commonly consumed salad leafy vegetables which were sold in Bangkok, and 2) to assess potential human health risks of As, Cd, and Pb exposure via salad leafy vegetables consumption. A total of 120 samples were randomly collected from local fresh markets in Bangkok. The total concentrations of toxic metals were determined by an inductively coupled plasma mass spectroscopy (ICP-MS). Total concentrations of As, Cd and Pb in coral lettuce (CL), red coral (RC) and green oak (GO) for soil cultivation …
The Application Of Machine Learning In Clustering Borderline Mild Cognitive Impairment Among Aging Thai People Living With Hiv, Akarin Hiransuthikul
The Application Of Machine Learning In Clustering Borderline Mild Cognitive Impairment Among Aging Thai People Living With Hiv, Akarin Hiransuthikul
Chulalongkorn University Theses and Dissertations (Chula ETD)
Many people living with HIV (PLWH) have cognitive impairment. Details of cognitive impairment subtypes are lacking. Unsupervised machine learning (ML) can reveal hidden subgroups within heterogeneous data. The study aimed to determine clusters of aging Thai PLWH with borderline cognitive impairment using unsupervised ML. HIV-NAT 207 study enrolled Thai PLWH aged ≥50 years. Cognitive performance was evaluated by the Thai-validated Montreal Cognitive Assessment (MoCA). This study included participants who scored between 23 and 27. The score of each cognitive domain served as cluster variables for the K-means algorithm. Among 340 PLWH, 177 (52.1%) scored between 23 and 27. Median age …
Analysis Of Inventory In A Food Repackaging Company, Chutipapha Thiantravan
Analysis Of Inventory In A Food Repackaging Company, Chutipapha Thiantravan
Chulalongkorn University Theses and Dissertations (Chula ETD)
This research aims to evaluate the nature of business and apply a suitable inventory policy to a case study food-repackaging company. The company adopted bulk-purchasing to packaging inventory to realize the economy of scales. Because of the lacks of purchasing standard and monitoring, this inventory policy led to the overstock, deadstock, and obsolete packaging inventories as well as the fluctuation of inventory turnover rate. The further category analysis of the packaging inventories using ABC analysis reveals that special customized bags and packaging items have a long lead time due to international sourcing and high minimum order quantity (MOQ). As a …
Spray Dried Asiatic Acid-Palm Oil Nanoemulsion With Improved Characteristics And Antihypertensive Effect Study In Rat Model Of Hypertension, Aditya Trias Pradana
Spray Dried Asiatic Acid-Palm Oil Nanoemulsion With Improved Characteristics And Antihypertensive Effect Study In Rat Model Of Hypertension, Aditya Trias Pradana
Chulalongkorn University Theses and Dissertations (Chula ETD)
Hypertension is a risk factor for premature death worldwide.It is characterized by an increase in blood pressure, so it needs therapy to regulate it and keep the value normal. Asiatic acid (AA) and palm oil (PO) were alternative antihypertensive agents that were promising to be developed. Both were natural compounds obtained from the Centella asiatica and Elaesis guineensis plants. However, low solubility, low absorption, rapid metabolism, low bioavailability and high oxidation were the limitation for both of them as an active compounds. Thus, a combination of nanoemulsion formulation and drying with a spray dryer was necessary to improve the physicochemical …
Assessments Of Chromium Pollution In Surface Sediment Of The Inner Gulf Of Thailand, Patcha Leelakun
Assessments Of Chromium Pollution In Surface Sediment Of The Inner Gulf Of Thailand, Patcha Leelakun
Chulalongkorn University Theses and Dissertations (Chula ETD)
The inner Gulf of Thailand is an important marine resource for economic and social developments of Thailand. Moreover, the inner Gulf of Thailand is a diverse of biological coastal ecosystem, which is make an equilibrium marine environment. On the other hand, this area is due mainly to a variety of pollutant sources from the surface runoff, particularly major large rivers including the Mae Klong, the Tha Chin, the Chao Phraya and the Bangpakong Rivers. As a result, chromium contamination can occur in the inner Gulf of Thailand and ultimately become a serious environmental issue in Thailand. Therefore, the surface sediments …
Influence Of Seasonal Variations On Contamination Level And Ecological Risk Of Heavy Metals In The Surface Sediments Of The Inner Gulf Of Thailand, Phuttiphat Lapat-Atiwat
Influence Of Seasonal Variations On Contamination Level And Ecological Risk Of Heavy Metals In The Surface Sediments Of The Inner Gulf Of Thailand, Phuttiphat Lapat-Atiwat
Chulalongkorn University Theses and Dissertations (Chula ETD)
The Inner Gulf of Thailand is a marine resource that provides ecological and economic value. Meanwhile, its can be acted like pollutions sink, especially heavy metals. After that, the extensions of industrialization, agriculturalization, and urbanization can degenerate to the inner Gulf environment quality. Therefore, this thesis was aimed to study the spatial distribution and seasonal variation of heavy metal concentration (Cd, Pb, Cu, Co, Ni, Mn and Zn) in surface sediment including the Mae Klong, the Tha Chin, the Chao Phraya and the Bangpakong River estuaries. Moreover, the contamination status and ecological risk were also evaluated. The results shown the …
Investigation Of Copper And Zinc Contaminations In Bioponic Systems Using Chicken Manure Fertilizer, Satja Aksorn
Investigation Of Copper And Zinc Contaminations In Bioponic Systems Using Chicken Manure Fertilizer, Satja Aksorn
Chulalongkorn University Theses and Dissertations (Chula ETD)
The bioponic system is a system that utilizes biological waste as a nutrient-rich source such as chicken manure fertilizer for plant growth integrated with aquaponic. At present, chicken manure fertilizer is contaminated with Cu and Zn may have the potential health effects through vegetable consumption. The objective of this study was to evaluate nitrogen transformation, nitrogen recovery, and plant growth in chicken manure-based bioponics at Cu (50–150 mg/kg) and Zn (200–600 mg/kg) supplementation and investigate the effects of Cu and Zn supplementations on plant bioaccumulation, root microbial community, and dietary health risk. Cu and Zn concentrations were analyzed using inductively …
Gis Mapping Evaluation Of Stroke Service Areas In Bangkok Using Emergency Medical Services, Kiatirat Sreemongkol
Gis Mapping Evaluation Of Stroke Service Areas In Bangkok Using Emergency Medical Services, Kiatirat Sreemongkol
Chulalongkorn University Theses and Dissertations (Chula ETD)
Nowadays, stroke is the leading cause of death in Thailand. Patients must be treated within 4.5 hours from symptom onset to increase recovery rate. Time is therefore a critical factor of the success or failure of the treatments. The objective of this research is to investigate the service coverage of various healthcare centers through current Emergency Medical Services (EMS) in Bangkok. To get service coverages for Stroke in Bangkok, the updated speed of the traffic and the amount of time it takes for each mode of transport for the pre-hospital stroke process are collected and examined using GIS mapping. The …
The Synergistic Activity Of Antibiotic Combinations Against Colistin-Resistant Acinetobacter Baumannii Clinical Isolates And Its Mechanisms Related To Colistin Resistance, Sukrit Srisakul
Chulalongkorn University Theses and Dissertations (Chula ETD)
The increasing development of colistin resistance in Acinetobacter baumannii clinical isolates is a global concern. Due to the limitation of effective antibiotics, the antibiotic combination therapies are the alternative choices. Here, we investigated the prevalence of colistin resistant A. baumannii (CoR-AB) clinical isolates and their resistance mechanisms, and examined the in vitro synergistic activities of the antibiotic combinations against CoR-AB. Among 341 carbapenem-resistant Acinetobacter spp., 317 (92.0%) were identified as A. baumannii by gyrB multiplex PCR. The rate of colistin resistance was 15.1% in A. baumannii isolates with MIC range of 0.125 – 64 mg/L. A total of 30 representative …
Identification Of Snp Barcode For Classify Carbapenem-Resistant Enterobacteriaceae (Cre) And Non-Cre Using Nanopore Sequencing, Nicha Sangpiromapichai
Identification Of Snp Barcode For Classify Carbapenem-Resistant Enterobacteriaceae (Cre) And Non-Cre Using Nanopore Sequencing, Nicha Sangpiromapichai
Chulalongkorn University Theses and Dissertations (Chula ETD)
Background: The continuous increase of carbapenem-resistant Enterobacteriaceae (CRE) prevalence has been a serious problem for public health worldwide. In the previous study, 25% of 3864 Klebsiella pneumoniae isolates were carbapenem-resistant. One of the most important risks of bacterial drug resistance is the improper use of antibiotics. The routine antimicrobial-susceptibility test requires overnight and has a turnaround time of approximately 2–3 days which delays the proper antibiotics. This study aims to develop a technique to classify CRE and non-CRE by SNP barcode. Method: Forty Klebsiella pneumoniae (KP) clinical isolates were collected from 2020–2021 at King Chulalongkorn Memorial Hospital. Twenty samples were …
Production Of Low Trans-Fatty Acid Margarine By Plasma Hydrogenation, Kunlanan Puprasit
Production Of Low Trans-Fatty Acid Margarine By Plasma Hydrogenation, Kunlanan Puprasit
Chulalongkorn University Theses and Dissertations (Chula ETD)
Margarine is a widely used raw material in the food and bakery industry. A successfully novel technique for refined palm olein hydrogenation to free trans-fat margarine production using non-thermal dielectric barrier discharge (DBD) plasma has been demonstrated. The new green method does not use chemicals as a catalyst, thus making it extremely environmentally friendly. This also performs well at room temperature and atmospheric pressure making it easier to manage. Two-electrode arrangement patterns were studied in this research: parallel plate and needle-in-tube. The results showed that using the parallel plate electrodes, 1.44% trans-fat (was detected) and a trans-fat generation rate of …
The Effects Of A Recurrent Stroke Prevention Program On Blood Pressure, Low Density Lipoprotein Cholesterol, And Blood Glucose Among Patients With First Ischemic Stroke, Orapin Jullmusi
Chulalongkorn University Theses and Dissertations (Chula ETD)
This cluster randomized control trial research with control group and repeated measure. The objectives of this study were to explore the effect of a recurrent stroke prevention program on blood pressure, low density lipoprotein cholesterol, and blood glucose among patients with first ischemic stroke. The participants were the patients who have been diagnosed with first ischemic stroke. They were recruited from two stroke units from two tertiary hospitals in public health region 2, Thailand. Sixty participants were randomly recruited and divided into experimental group (n=30) and control group (n=30). The participants in the experimental group received the recurrent stroke prevention …
Efficacy Of Cannabidiol (Cbd) For The Treatment Of Recurrent Aphthous Ulcers, Chalapinyo Umpreecha
Efficacy Of Cannabidiol (Cbd) For The Treatment Of Recurrent Aphthous Ulcers, Chalapinyo Umpreecha
Chulalongkorn University Theses and Dissertations (Chula ETD)
The objectives of this research were 1) To investigate the allergic reactions to cannabidiol (CBD) when used on human skin. 2) To assess local and systemic side effects of CBD when used on normal oral mucosa. 3) To compare the efficacy among 0.1% CBD, 0.1% triamcinolone acetonide (TA), and placebo (pure oral paste) in recurrent aphthous ulcer (RAU) treatment. This project was composed of 3 phases. Phase 1, a skin patch test was performed on 100 healthy subjects. Phase 2, CBD was applied to normal oral mucosa of 50 healthy subjects 3 times/day for 7 days. Oral examination, vital signs, …
Effects Of Intrauterine Intermittent Hypoxia On Skeletal Muscle In Offspring Rats, Wirongrong Wongkitikamjorn
Effects Of Intrauterine Intermittent Hypoxia On Skeletal Muscle In Offspring Rats, Wirongrong Wongkitikamjorn
Chulalongkorn University Theses and Dissertations (Chula ETD)
Gestational intermittent hypoxia (IH), a hallmark of OSA, alters the offspring's respiratory neural control and diaphragm contractile function. Thus, we aimed to investigate the effects of gestational IH on the muscle development and metabolism of geniohyoid (GH), which is innervated by the respiratory-related hypoglossal nerve and plays a role in tongue traction and suckling, in male offspring rats compared with masseter (MAS), the largest masticatory muscle. Pregnant Sprague-Dawley rats were exposed to IH (3-min periods of 4–21% O2) compared to Normoxia for 8 hours/day during gestational days 7–20. GH and MAS from 35-day-old male offspring (n = 6 /group) were …
Production Of Bio-Olefins From Oleic Acidvia Oxidative Dehydrogenation Using Vanadium Oxides/Sba-15 Catalysts, Nattaporn Chaidherasuwet
Production Of Bio-Olefins From Oleic Acidvia Oxidative Dehydrogenation Using Vanadium Oxides/Sba-15 Catalysts, Nattaporn Chaidherasuwet
Chulalongkorn University Theses and Dissertations (Chula ETD)
Long-chain olefins (≥C10) are important commodity chemicals, which are primarily used to manufacture everyday products such as plastic packaging, surfactants and detergents in cleaning products, and lubricant oil additives. However, the long-chain olefins are currently synthesized from the petroleum refining process via ethylene oligomerization or thermal cracking of long-chain paraffins. To support the Bio-Circular-Green Economy (BCG) model, the direct synthesis of olefins from renewable feedstocks has become an attractive route to promote overall sustainability. The objective of this research was to study the possibility to transform oleic acid (OA), one of the unsaturated fatty acids in palm oil, to produce …
Clinical Efficacy Of Silk Sericin Dressing With Collagen For Split-Thickness Skin Graft Donor Site Treatment, Apirujee Punjataewakupt
Clinical Efficacy Of Silk Sericin Dressing With Collagen For Split-Thickness Skin Graft Donor Site Treatment, Apirujee Punjataewakupt
Chulalongkorn University Theses and Dissertations (Chula ETD)
Sericin dressing containing collagen hydrolysate (SDC) was evaluated in this study, consisting of two phases of clinical trials. The clinical safety of SDC was determined using a patch test in 103 healthy volunteers (phase I). Most of the results (around 90%) in the SDC group were interpreted as negative and doubtful reactions, and the remaining results (around 10%) were weak reactions. After that, the clinical efficacy and safety of SDC were investigated in 21 patients with 30 split-thickness skin graft (STSG) donor sites (phase II). Each donor site was divided into two equal sites. SDC or the commercial dressing (Bactigras®) …
Identification, Antifungal And Plant Growth Promoting Activities Of Endophytic Actinomycetes From Orchids, Nisachon Tedsree
Identification, Antifungal And Plant Growth Promoting Activities Of Endophytic Actinomycetes From Orchids, Nisachon Tedsree
Chulalongkorn University Theses and Dissertations (Chula ETD)
Sixty-two endophytic actinomycetes were isolated from 15 Thai orchids. They were identified as Streptomyces (34 isolates), Micromonospora (21 isolate), Streptosporangium (2 isolates), Actinomadura (1 isolates), Amycolatopsis (3 isolates) and Pseudonocardia (1 isolates) based on their phenotypic, chemotaxonomic and genotypic characteristics. The antimicrobial activity screening revealed that Streptomyces (18 isolates), Micromonospora (12 isolates) and Streptosporangium (1 isolate) exhibited antimicrobial activity against Bacillus subtilis ATCC 6633, Kocuria rhizophila ATCC 9341, Staphylococcus aureus ATCC 25923, Escherichia coli ATCC 25922, Pseudomonas aeruginosa ATCC 27853 and Candida albicans ATCC 10231. Strain DR2-2 could inhibit all tested pathogens. Based on 16S rRNA gene sequencing, strain DR6-1 …
Extraction Of Cannabinoids Using Vegetable Oils And Its Uva-Photoprotective Effect On Human Skin Keratinocytes, Nur Alfan Muhammad Zen
Extraction Of Cannabinoids Using Vegetable Oils And Its Uva-Photoprotective Effect On Human Skin Keratinocytes, Nur Alfan Muhammad Zen
Chulalongkorn University Theses and Dissertations (Chula ETD)
Nowadays, vegetable oil has attracted attention in its role as an alternative solvent. Its ability to dissolve various active compounds from plants has been proven by several previous studies. Moreover, other benefits of the use of vegetable oil as a solvent are affordable, non-toxic, and eco-friendly. The extraction process is one of the crucial factors that affect the quality of cannabis. Ethanol is still consistently the best solvent for cannabis extraction in terms of yield production compared to other organic solvents used in this study including oil whose yield cannot be calculated since vegetable oil cannot be evaporated. HPTLC fingerprint …
Effects Of Low Degree Of High Ambient Temperature On Neural Pathways From Median Preoptic Nucleus To Arcuate Nucleus In Related To Food Intake., Pornsiri Suwannapaporn
Effects Of Low Degree Of High Ambient Temperature On Neural Pathways From Median Preoptic Nucleus To Arcuate Nucleus In Related To Food Intake., Pornsiri Suwannapaporn
Chulalongkorn University Theses and Dissertations (Chula ETD)
High ambient temperature (HTa) is one important environmental factor that decreases food intake (FI). It is well accepted that high degree HTa induced heat stress decreased FI by the activation of corticotropin-releasing factor (CRF) via hypothalamic pituitary adrenal axis. However, short-term low-degree HTa exposure has been demonstrated to decrease FI earlier than activated physiological and stress responses. This effect was related to the neuronal connection between the median preoptic nucleus (MnPO) and arcuate nucleus (Arc). Therefore, the present study aimed to investigate the effect of short-term low-degree HTa exposure on the activity of hypothalamic Arc in regarding to FI, and …