Open Access. Powered by Scholars. Published by Universities.®
- Discipline
-
- Medicine and Health Sciences (3086)
- Social and Behavioral Sciences (981)
- Medical Sciences (830)
- Engineering (502)
- Physical Sciences and Mathematics (489)
-
- Nursing (445)
- Education (401)
- Dentistry (357)
- Life Sciences (355)
- Pharmacy and Pharmaceutical Sciences (342)
- Sports Sciences (286)
- Veterinary Medicine (284)
- Public Health (272)
- Business (246)
- Economics (220)
- Sociology (220)
- Chemistry (191)
- Arts and Humanities (183)
- Architecture (181)
- Psychology (161)
- Law (156)
- Health and Physical Education (145)
- Mental and Social Health (145)
- Other Mental and Social Health (143)
- Public Affairs, Public Policy and Public Administration (136)
- Environmental Sciences (133)
- Civil and Environmental Engineering (126)
- Communication (120)
- Communication Technology and New Media (120)
- Mass Communication (120)
- Keyword
Articles 5851 - 5880 of 6481
Full-Text Articles in Entire DC Network
Factors Influencing On Nursing Care Quality Perceived By Professional Nurses In Government Hospitals, Kingdom Of Cambodia, Virya Koy
Chulalongkorn University Theses and Dissertations (Chula ETD)
This descriptive cross-sectional research design for causal modeling aimed to examine factors influencing on nursing care quality in government hospitals, Cambodia. The conceptual framework were modified from the Nurse Work Environment, Nurse Staffing, and Outcome Model and empirical studies. A multi-stage sampling was used for data collection, which conducted from October 2016 to April 2017. Three hundred and seventy five RNs represented twelve tertiary general hospitals were invited for the study. All of participants completed five questionnaires, including demographic data and nurse staffing, nurse practice environment, nursing work index, Copenhagen burnout inventory, Index work satisfaction, and Cambodian nursing care quality …
Effect Of Curcumin On The Expression Of Wound Healing-Related Genes In Human Gingival Fibroblasts, Auspreeya Rujirachotiwat
Effect Of Curcumin On The Expression Of Wound Healing-Related Genes In Human Gingival Fibroblasts, Auspreeya Rujirachotiwat
Chulalongkorn University Theses and Dissertations (Chula ETD)
This study investigated the effect of curcumin on the expression of wound healing-related genes including transforming growth factor beta 1 (TGF-β1), transforming growth factor beta receptor type I (TGFβR I), transforming growth factor beta receptor type II (TGFβR II) and vascular endothelial growth factor (VEGF) in human gingival fibroblasts. The cytotoxicity of curcumin was determined by MTT assay. Then, cells were treated with non-cytotoxic concentrations of curcumin for 24 hours and the level of gene expression was determined by quantitative polymerase chain reaction (qPCR). Curcumin at 0.1-20 µM caused no significant change in cell viability while higher concentrations of curcumin …
Factors Associated With Dental Caries Experience In Thai Preschool Children With Cerebral Palsy Attending Non-Governmental Rehabilitation Centers, Kullanant Pansrimangkorn
Factors Associated With Dental Caries Experience In Thai Preschool Children With Cerebral Palsy Attending Non-Governmental Rehabilitation Centers, Kullanant Pansrimangkorn
Chulalongkorn University Theses and Dissertations (Chula ETD)
Objective: To investigate factors associated with dental caries experience in preschool children with cerebral palsy attending physical therapy programs in non-governmental rehabilitation centers in Bangkok. Materials and Methods: This research is a cross sectional study of 60 children with cerebral palsy, aged 3-6 years, mean age 5.01 ± 1.23 years old. Dental caries experience was determined by dmfs index, numbers of surfaces of dental caries, extracted tooth, and filled tooth. The other variables were collected including: dental plaque index (OHI-S), type of cerebral palsy, frequency of carbohydrate intake events, dietary consistency, assisting with toothbrushing, and frequency of toothbrushing. Multiple linear …
Toxicity Of Silver, Silver-Copper And Silver-Tin Nanoparticles In Thp-1 Cell Line And Thp-1 Differentiated Macrophage Cells, Patamaporn Monprasit
Toxicity Of Silver, Silver-Copper And Silver-Tin Nanoparticles In Thp-1 Cell Line And Thp-1 Differentiated Macrophage Cells, Patamaporn Monprasit
Chulalongkorn University Theses and Dissertations (Chula ETD)
Silver nanoparticles (AgNPs) and nanoparticles in the form of silver-metal alloy, such as silver-copper (AgCuNPs) and silver-tin (AgSnNPs) have been applied in various industries and medical applications. To assess effects of these nanoparticles on human immune system, this study aimed to assess toxicity of AgNPs, AgCuNPs and AgSnNPs on human monocytes, THP-1 cells, and THP-1 differentiated macrophage cells. The nanoparticles were characterized and assessed for cytotoxicity and generation of intracellular ROS. Effects of the nanoparticles on the release of cytokines (TNF-α, IL-1β and IL-8) were also assessed. The results demonstrated no significant cytotoxicity of silver and silver-metal nanoparticles at the …
Enhancing H2s Sensing Response Of Calcium Copper Titanate Thin Films By Adding Ag Compounds, Aukrit Natkaeo
Enhancing H2s Sensing Response Of Calcium Copper Titanate Thin Films By Adding Ag Compounds, Aukrit Natkaeo
Chulalongkorn University Theses and Dissertations (Chula ETD)
Ag-doped CaCu3Ti4O12 (CCTO) thin films with different doping concentrations were prepared by a sol-gel technique. Films were grown by depositing four layers of CCTO on alumina substrates followed by sputtering Au/Cr interdigitated electrodes to fabricate gas sensors. The films were characterized by X-ray diffraction (XRD), field emission scanning electron microscopy (FESEM), energy dispersive X-ray spectroscopy (EDX) Raman spectroscopy and X-ray photoemission spectroscopy (XPS). The films have predominantly the perovskite CCTO crystal phase with very small amount of TiO2 secondary phases. In this thesis, both undoped CCTO and Ag-doped CCTO thin films were tested for selective sensing to H2S gas relative …
Subjective And Objective Outcomes On Denture Adhesive Usage Among Complete Denture Wearers, Budsara Thongyoi
Subjective And Objective Outcomes On Denture Adhesive Usage Among Complete Denture Wearers, Budsara Thongyoi
Chulalongkorn University Theses and Dissertations (Chula ETD)
The propose of this clinical study was to evaluate the subjective and objective assessment factors affecting decision of complete denture wearers to use or not use denture adhesive. Sixty-six fully edentulous using upper and lower conventional complete dentures (aged 53 to 83 years) were recruited in this study. (1) Condition of denture-supporting tissue (ACP classification), (2) denture quality (CU-modified kapur criteria), (3) patients' satisfaction in prostheses and (4) oral impact on daily performances (OIDP) were evaluated as baseline (T0). All participants were assigned to use denture adhesive (Polident®, Ireland) for 1 month period and made decision by themselves to continue …
The Synthesis, Modification And Application Of Silica Nanoparticles In Polymethyl Methacrylate Denture Base, Pornpot Jiangkongkho
The Synthesis, Modification And Application Of Silica Nanoparticles In Polymethyl Methacrylate Denture Base, Pornpot Jiangkongkho
Chulalongkorn University Theses and Dissertations (Chula ETD)
This study aimed to investigate amount of γ-methacryloxypropyltrimethoxysilane (MPS) silanized on experimental nanosilica particles (NPs), amount of NPs and amount of MPS silanized NP on flexural strength (FS), flexural modulus (FM), and fracture toughness (FT) of NP reinforced polymethymethacrylate (PMMA). The chemisorbed amount of MPS was determined using elemental analysis. Six groups (n=8) were prepared with chemisorbed amount and mixed with PMMA-monomer to make 0.25, 0.5, 1, 5, 10 and 15% (w/w) of NP reinforced PMMA. PMMA without NP served as control. Seven groups (n=8) were prepared with 1% of NPs silanized with 0, 0.061, 0.123, 0.246, 0.493, 0.987, and …
Factors Influencing Quality Of Life Among Elderly Population With Type 2 Diabetes Mellitus: A Clinic Based Cross Sectional Study In Kathmandu Valley, Kriti Adhikari
Chulalongkorn University Theses and Dissertations (Chula ETD)
Background: Diabetes is one of the four priority non communicable disease whose worldwide prevalence is at an increasing trend and is accompanied by deterioration of quality of life especially that of the elderly population. Several factors contribute to influence quality of life among the elderly; therefore, this study aims to identify the factors that influence quality of life among elderly population of Kathmandu valley, Nepal Methods: A cross sectional survey was conducted among random sample of 310 elderly diabetic patients visiting diabetic clinic of Kathmandu Diabetes and Thyroid center in Kathmandu valley, Nepal. Translated Nepali version of WHOQOL BREF was …
ตัวแบบการกำหนดราคาของการประกันภัยโรคร้ายแรงโดยใช้อัตราความชุกในประเทศไทย, รติกร แย้มสรวล
ตัวแบบการกำหนดราคาของการประกันภัยโรคร้ายแรงโดยใช้อัตราความชุกในประเทศไทย, รติกร แย้มสรวล
Chulalongkorn University Theses and Dissertations (Chula ETD)
งานวิจัยนี้มีวัตถุประสงค์เพื่อประมาณค่าความรุนแรงของการเปลี่ยนสถานะสำหรับการประกันภัยโรคร้ายแรงและคำนวณหาอัตราเบี้ยประกันภัยสุทธิสำหรับการประกันภัยโรคร้ายแรงที่คุ้มครอง 6 โรค ได้แก่ โรคมะเร็งระยะลุกลาม โรคกล้ามเนื้อหัวใจตายเฉียบพลันจากการขาดเลือด โรคหลอดเลือดในสมองแตกหรืออุดตัน โรคไตวายเรื้อรัง โรคตับวายและโรคหลอดลมปอดอุดกั้นเรื้อรังขั้นรุนแรง โดยใช้อัตราความชุกในการประมาณค่าอัตราอุบัติการณ์ของโรคร้ายแรงที่ไม่ทราบค่า ซึ่งใช้ตัวแบบหลายสถานะ(multiple state model) และฟังก์ชันคงตัวเป็นช่วง (piecewise constant function) ในการประมาณค่าความรุนแรงของการเปลี่ยนสถานะจากสถานะสุขภาพดีไปยังสถานะเจ็บป่วยด้วยโรคร้ายแรงและสมมติให้ความรุนแรงของเสียชีวิตของทั้งคนสุขภาพดีและผู้ป่วยด้วยโรคร้ายแรงเป็นไปตามตัวแบบ Gompertz-Makeham (GM) ข้อมูลที่ใช้ในงานวิจัยคือ 1) จำนวนประชากรกลางปี 2) จำนวนการตายของประชากรไทย จำแนกตามกลุ่มอายุและเพศ 3) จำนวนการตาย และ 4) จำนวนการป่วย จำแนกตามกลุ่มอายุ เพศและสาเหตุการตายและการป่วยตามบัญชีจำแนกโรคระหว่างประเทศฉบับแก้ไขครั้งที่ 10 (ICD-10) ตั้งแต่ปี พ.ศ.2554-2558 จากสำนักนโยบายและยุทธศาสตร์ กระทรวงสาธารณสุข ผลการศึกษาพบว่าความรุนแรงของการเปลี่ยนสถานะจากสถานะเจ็บป่วยด้วยโรคร้ายแรงไปยังสถานะเสียชีวิตเนื่องจากโรคร้ายแรงประมาณได้ด้วยตัวแบบ GM(4,0) ทั้งเพศชายและเพศหญิง และจากสถานะเจ็บป่วยด้วยโรคร้ายแรงไปยังสถานะเสียชีวิตเนื่องจากสาเหตุอื่น ๆ นอกเหนือจากโรคร้ายแรง สามารถประมาณได้ด้วยตัวแบบ GM(1,2) และ GM(2,2) สำหรับเพศชายและหญิงตามลำดับ เบี้ยประกันภัยสุทธิจ่ายครั้งเดียวสำหรับการประกันภัยโรคร้ายแรงแผนกำหนดผลประโยชน์ไม่ครอบคลุมการเสียชีวิต (Stand-alone benefit) และแผนกำหนดผลประโยชน์ครอบคลุมการเสียชีวิต (Acceleration benefit) มีค่าเพิ่มขึ้นเมื่ออายุมากขึ้นและเบี้ยประกันภัยสำหรับเพศชายมีค่าสูงกว่าเพศหญิงทุกกลุ่มอายุ
การพัฒนาโมเดลวินิจฉัยความเข้าใจในการอ่านภาษาไทยของนักเรียนชั้นประถมศึกษาปีที่ 6 : การประยุกต์ใช้โมเดลฟิวชัน, ธนิยา เยาดำ
การพัฒนาโมเดลวินิจฉัยความเข้าใจในการอ่านภาษาไทยของนักเรียนชั้นประถมศึกษาปีที่ 6 : การประยุกต์ใช้โมเดลฟิวชัน, ธนิยา เยาดำ
Chulalongkorn University Theses and Dissertations (Chula ETD)
การวิจัยครั้งนี้มีวัตถุประสงค์เพื่อ 1) พัฒนาทักษะความเข้าใจในการอ่านภาษาไทยของนักเรียนชั้นประถมศึกษาปีที่ 6 2) พัฒนาแบบทดสอบความเข้าใจในการอ่านภาษาไทยของนักเรียนชั้นประถมศึกษาปีที่ 6 3) ตรวจสอบคุณภาพแบบทดสอบความเข้าใจในการอ่านภาษาไทยของนักเรียนชั้นประถมศึกษาปีที่ 6 และ 4) วินิจฉัยความเข้าใจในการอ่านภาษาไทยของนักเรียนชั้นประถมศึกษาปีที่ 6 โดยใช้โมเดลฟิวชัน กลุ่มตัวอย่าง ประกอบด้วย (1) ผู้เชี่ยวชาญ 17 คน เพื่อพัฒนาทักษะความเข้าใจในการอ่าน (2) นักเรียนชั้นประถมศึกษาปีที่ 6 จำนวน 818 คน เพื่อวินิจฉัยความเข้าใจในการอ่าน เครื่องมือมี 3 ฉบับ คือ แบบสอบถาม 2 ฉบับ สำหรับผู้เชี่ยวชาญเป็นสอบถามเกี่ยวกับทักษะความเข้าใจในการอ่าน โดยชุดที่ 1 เป็นแบบสอบถามปลายเปิด ชุดที่ 2 เป็นแบบสอบถามปลายปิด และแบบทดสอบความเข้าใจในการอ่านภาษาไทย 1 ฉบับ สำหรับนักเรียน วิเคราะห์ข้อมูลโดยใช้สถิติเชิงบรรยาย ได้แก่ ร้อยละ ค่าเฉลี่ย และส่วนเบี่ยงเบนมาตรฐาน วิเคราะห์ทักษะความเข้าใจในการอ่านภาษาไทย หาฉันทามติด้วยค่ามัธยฐาน ผลต่างของมัธยฐานกับฐานนิยม และพิสัยระหว่างควอไทล์ วิเคราะห์โมเดลวินิจฉัยความเข้าใจในการอ่านโดยใช้โมเดลฟิวชัน ผลการวิจัยพบว่า ความเข้าใจในการอ่านภาษาไทยมี 7 ทักษะ ดังนี้ 1) การบอกความหมายของคำศัพท์โดยไม่อาศัยบริบท 2) การบอกความหมายของคำศัพท์โดยอาศัยบริบท 3) การจับใจความรองโดยไม่อาศัยบริบท 4) การจับใจความรองโดยอาศัยบริบท 5) การจับใจความหลักโดยไม่อาศัยบริบท 6) การจับใจความหลักโดยอาศัยบริบท และ 7) การตีความ ทักษะความเข้าใจในการอ่านภาษาไทย 7 ทักษะ ผู้เชี่ยวชาญมีความคิดเห็นเป็นฉันทามติในระดับมากที่สุด (Md=5.00, |Md-Mo|=0, IR=0) แบบทดสอบความเข้าใจในการอ่านภาษาไทยของนักเรียนชั้นประถมศึกษาปีที่ 6 ที่ครอบคลุมทั้ง 7 ทักษะ มีจำนวนข้อสอบ 23 ข้อ เป็นแบบทดสอบแบบอิงสถานการณ์ รูปแบบข้อสอบเป็นแบบเลือกตอบ 4 ตัวเลือก คุณภาพของข้อสอบจากการวิเคราะห์ด้วยโปรแกรม MULTILOG มีค่าความยากง่าย (b) …
Chemical Constituents From The Roots Of Calophyllum Calaba L., Fuengfa Laopian
Chemical Constituents From The Roots Of Calophyllum Calaba L., Fuengfa Laopian
Chulalongkorn University Theses and Dissertations (Chula ETD)
Phytochemical investigation of the CH2Cl2 extract from the roots of C. calaba let to the isolation of three new xanthone derivatives, namely calaxanthones A-C (1-3), along with twenty nine known xanthones (4-32). The structures of all isolated compounds were fully characterized using spectroscopic data (1D and 2D NMR) as well as comparison with the previous literature data. Moreover, all isolated compounds were assessed for their in vitro cytotoxicity against the KB, HeLa S-3, HT29, MCF-7 and HepG2 human cancer cell lines. The tested compounds mostly showed moderate to inactive against these five cell lines, except compounds 3 showed potent cytotoxicity …
Effects And Mechanisms Of Porcine Reproductive And Respiratory Syndrome Virus (Prrsv) Type 1 And Type 2 On The Permeability And Viability Of Porcine Endometrial Epithelial Cells, Dran Rukarcheep
Chulalongkorn University Theses and Dissertations (Chula ETD)
Both PRRSV types 1 and 2 revealed the sign of reproductive disorders associated with a lesion at implantation sites. Impairment of maternal glandular endometrium cell integrity and function by PRRSV infection may impact a proper nourishing fetus. This research was aimed to examine the effects of PRRSV type 1 and type 2 directly on the viability and barrier function of the endometrium using porcine glandular endometrial epithelial cell culture (PEG). The comparison of the route and the strain of PRRSV infection coinciding with their virulent in reproductive epithelia were considered. PEG cells isolated from the 4-6 months old PRRSV-free-herd gilts …
Potentials Of Ivabradine To Improve Cardiac Function In Dogs With Naturally Occurring, Asymptomatic Degenerative Mitral Valve Disease, Prapawadee Pirintr
Potentials Of Ivabradine To Improve Cardiac Function In Dogs With Naturally Occurring, Asymptomatic Degenerative Mitral Valve Disease, Prapawadee Pirintr
Chulalongkorn University Theses and Dissertations (Chula ETD)
The main hypothesis of the present study is that ivabradine (1.0 mg/kg, orally, twice daily) can reduce heart rate (HR), myocardial oxygen consumption (MVO2) and improve cardiac function in dogs with degenerative mitral valve disease (DMVD) partly due to a reduction of cardiomyocyte apoptosis. In order to test the hypothesis, this study was divided into three parts. The first part aimed to determine the appropriate single oral dose of ivabradine for reduction of HR and MVO2 as assessed by rate-pressure product (RPP= HR x systolic blood pressure). Once the appropriate dose was achieved, the second part was conducted to investigate …
Potentials Of Ivabradine To Improve Cardiac Function In Dogs With Naturally Occurring, Asymptomatic Degenerative Mitral Valve Disease, Prapawadee Pirintr
Potentials Of Ivabradine To Improve Cardiac Function In Dogs With Naturally Occurring, Asymptomatic Degenerative Mitral Valve Disease, Prapawadee Pirintr
Chulalongkorn University Theses and Dissertations (Chula ETD)
The main hypothesis of the present study is that ivabradine (1.0 mg/kg, orally, twice daily) can reduce heart rate (HR), myocardial oxygen consumption (MVO2) and improve cardiac function in dogs with degenerative mitral valve disease (DMVD) partly due to a reduction of cardiomyocyte apoptosis. In order to test the hypothesis, this study was divided into three parts. The first part aimed to determine the appropriate single oral dose of ivabradine for reduction of HR and MVO2 as assessed by rate-pressure product (RPP= HR x systolic blood pressure). Once the appropriate dose was achieved, the second part was conducted to investigate …
Effects Of Short-Term Growth Hormone Administration On Plasma Leptin Levels In Normal And Diet-Induced Obesity Rats, Sutharinee Likitnukul
Effects Of Short-Term Growth Hormone Administration On Plasma Leptin Levels In Normal And Diet-Induced Obesity Rats, Sutharinee Likitnukul
Chulalongkorn University Theses and Dissertations (Chula ETD)
Obesity has related to the alteration of hormonal profiles, i.e. growth hormone (GH), insulin and leptin. Because of the pleiotropic mechanisms of GH, it plays important roles both in the pathophysiological mechanisms and the therapeutic hormonal supplementation, which probably affected plasma leptin. The present research aimed firstly to demonstrate the effect of short-term GH administration on plasma leptin in 3 conditions; basal, meal-induced and fasting condition, from the rats with different adipose tissue mass. Secondly, to investigate short-term effect of GH on insulin sensitivity and body adiposity in control, diet resistant (DR) and diet-induced obesity (DIO) rats. In this regard, …
The Protective Effect Of Hydroxyxanthone On The Leakiness Of Intestinal Epithelia By The Proinflammatory Cytokine Related To Bacterial Endotoxin, Wannaporn Chayalak
The Protective Effect Of Hydroxyxanthone On The Leakiness Of Intestinal Epithelia By The Proinflammatory Cytokine Related To Bacterial Endotoxin, Wannaporn Chayalak
Chulalongkorn University Theses and Dissertations (Chula ETD)
Leaky gut characterized as decreased transepithelial electrical resistance and increased paracellular permeability to macromolecules is associated with several fatal diseases including sepsis. Pro-inflammatory cytokine IL-1β is a critical mediator of underlying mechanism through activation of myosin light chain kinase pathway. Blocking the inducible phosphorylated myosin light chain (p-MLC) indicates the successful treatment. Xanthones predominantly found in mangosteen (Garcinia mangostana) has been indicated as a potent anti-inflammatory action. Hydroxyxanthones (HDX), the major natural xanthones, have been recently synthesized in various forms. This study aimed to examine and screen the action of various forms of synthetic HDXs, in preventing the increased paracellular …
Pulmonary Lesion Texture Classification From Endobronchialultrasonogram Using Texture Analysis, Banphatree Khomkham
Pulmonary Lesion Texture Classification From Endobronchialultrasonogram Using Texture Analysis, Banphatree Khomkham
Chulalongkorn University Theses and Dissertations (Chula ETD)
This research aims to develop a method to help distinguish the appearance of pulmonary lesions from a high-frequency sound (Endobronchial Ultrasound—EBUS) image. According to medical information, the appearance of smooth or rough texture of a lesion can significantly indicate that it is malignant or benign. In this study, the features that are used in the classification are divided into 3 groups: group 1 consists of 22 standard features, group 2 consists of the proposed features extracted from the weighted sum of the upper and lower GLCM which consists 12 features, and group 3 is the combination of group 1 and …
Mathematical Models For Treatment Time Of Pathogenic Infection In Blood After Antibiotics Administration, Panit Suavansri
Mathematical Models For Treatment Time Of Pathogenic Infection In Blood After Antibiotics Administration, Panit Suavansri
Chulalongkorn University Theses and Dissertations (Chula ETD)
The objective in this thesis is to create a mathematical model to predict the period of time needed to clear out the pathogens from the bloodstream after the patient receives certain antibiotics. The result will lead to the time where the drug administration can be terminated. This research is to compute the densities of bacteria and malaria in bloodstream at any given time and to compare this level with the actual results of the patient's blood sampling, which was sent to the laboratory for investigation. The laboratory process of the blood sampling will usually take more than 2-3 days to …
3d Printing Of Graphene/Thermoplastic Elastomer Composites, Warrayut Kanabenja
3d Printing Of Graphene/Thermoplastic Elastomer Composites, Warrayut Kanabenja
Chulalongkorn University Theses and Dissertations (Chula ETD)
Graphene based thermoplastic polyurethane composites filaments was prepared by melt blending process prior to fabricate with 3D printing technique. The effects of different graphene loading (0.05 wt% - 0.20 wt%) in TPU matrix on the properties of composites were studied. Initially, graphite was successfully oxidized to graphite oxide following Hummer’s method and the reduction of graphene oxide to graphene by L-ascorbic acid was also achieved as being confirmed by the FTIR and XRD results. TPU and TPU/graphene nanocomposites were investigated for their thermal, mechanical, physical and electrical properties. Thermal stability of TPU/graphene composites was improved against neat TPU. The first …
Revitalizing Tio Chew Chinese Cemetery Through A Design Of An Elderly Center, Khai Sin Lee
Revitalizing Tio Chew Chinese Cemetery Through A Design Of An Elderly Center, Khai Sin Lee
Chulalongkorn University Theses and Dissertations (Chula ETD)
Old cemeteries are often threatened by new urban development, which can result in them being closed, relocated or demolished. This thesis studies Tio Chew Cemetery in Bangkok as an alternative case based on its current unique use as a recreation area. Although without the threats of demolition, several on-site issues, both tangible and intangible, are facing Tio Chew Cemetery. To respond to these issues, a design of an elderly center is proposed to improve and revitalize the cemetery through the understanding of historical importance and contemporary design of new buildings in the old compound. This research applies a mixed methodology …
Tropical Strategy Of Modern Architecture In Cambodia For The Design Of A Contemporary Art Space In Phnom Penh, Pisith Ty
Chulalongkorn University Theses and Dissertations (Chula ETD)
In the past decades, Cambodia has experienced a boom in building construction. However, many of those buildings do not take sustainable approach into account. They are built without concern of the tropical climate which results into a high level of energy consumption from air-conditioning system. Some none-commercial buildings, such as cultural and educational facilities, could not be operated because of lack of the fund for electricity. For a better understanding of architectural design in the tropical environment of the context, the research focuses on the works of Vann Molyvann, the father of architects in Cambodia, a state architect in 1960s, …
Expression And Characterization Of Α-Glucosidase From Weissella Confusa Bbk-1, Lalita Silapasom
Expression And Characterization Of Α-Glucosidase From Weissella Confusa Bbk-1, Lalita Silapasom
Chulalongkorn University Theses and Dissertations (Chula ETD)
Alpha-glucosidase [EC 3.2.1.20; α-D-glucoside glucohydrolase] is an exohydrolase which catalyzes non-reducing end of substrates to release D-glucose. In addition, alpha-glucosidase also displays a transferase activity, which bring about the formation of α-glucosylated compounds such as soluble starch and glycogen. This research aims to express and characterize α-glucosidase from Weissella confusa BBK-1 (WcAG). WcAG gene was expressed in modified pET28b vector. This gene contained an open reading frame of 1,775 bps. Optimum expression condition of WcAG was cultured in LB medium containing 1% (w/v) glucose and induced with 0.4 mM IPTG at 20°C for 20 h. The recombinant WcAG containing his-tag …
Isolation And Characterization Of Triclocarban Degrading Bacteria As Plant Growth Promoter, Merry Krisdawati Sipahutar
Isolation And Characterization Of Triclocarban Degrading Bacteria As Plant Growth Promoter, Merry Krisdawati Sipahutar
Chulalongkorn University Theses and Dissertations (Chula ETD)
Triclocarban (TCC) is a widely used as antimicrobial agent in household products. High usage volume of TCC results in its accumulation in sludge and lead to contamination in environments. Application of biosolid in agricultural activities leads to contamination of TCC in agricultural areas. So. development of bioremediation technique for TCC is necessary to maintain quality of agricultural ecosystem. For that purpose, this research isolated 5 soil bacteria capable of TCC degradation and with plant-growth promoting (PGP) activities including Ochrobactrum sp. MC22, Ochrobactrum sp. MC35, Sphingobacterium sp. MC43, Pseudomonas sp. MS45, and Pseudomonas fluorescens MC46. Among them, Ochrobactrum sp. MC22 and …
Effects Of Curcumin-Loaded Bacterial Cellulose Films On Cancer Cells, Chayut Subtaweesin
Effects Of Curcumin-Loaded Bacterial Cellulose Films On Cancer Cells, Chayut Subtaweesin
Chulalongkorn University Theses and Dissertations (Chula ETD)
In this study, a curcumin-loaded bacterial cellulose films were developed. Bacterial cellulose films were prepared by culturing Gluconacetobacter xylinus in coconut water-based medium. Curcumin, an active substance found in turmeric (Curcuma longa Linn), was then absorbed into never-dried bacterial cellulose pellicles by immersion in 0.5 and 1.0 mg/ml curcumin solutions, with absolute ethanol as solvent, for 24 hours. The curcumin-loaded bacterial cellulose pellicles were then air-dried. Controlled release of curcumin was achieved in buffer solutions containing Tween 80 and methanol additives, at pH 5.5 and 7.4. Curcumin-loaded bacterial cellulose films prepared with curcumin solutions at concentrations of 0.5 and 1.0 …
Prevalence And Molacular Genetic Analysis Of Human Respiratory Syncytial Virus And Enterovirus 68 Among Children With Acute Lower Respiratory Tract Infection In Thailand, I-Lada Thongpan
Chulalongkorn University Theses and Dissertations (Chula ETD)
Respiratory syncytial virus (RSV) causes acute lower respiratory tract infection in infants and young children worldwide. A clear description of local RSV molecular epidemiology, evolution, and transmission requires detailed sequence data and can inform new strategies for virus control and vaccine development. To investigate the RSV burden in Thailand over six consecutive years (January 2012 to December 2017). There were two different methods to detect RSV in the present study. First, we screened 3306 samples obtained from children ≤5 years old with acute respiratory tract infection using semi-nested reverse-transcription polymerase chain reaction (RT-PCR) during January 2012 to December 2015. Second, …
Pathomechanism Of X-Linked Osteogenesis Imperfecta, Siraprapa Tongkobpetch
Pathomechanism Of X-Linked Osteogenesis Imperfecta, Siraprapa Tongkobpetch
Chulalongkorn University Theses and Dissertations (Chula ETD)
Osteogenesis imperfecta (OI) or brittle bone disease is a genetic disorder characterized by deformities of bones and multiple bone fractures. We identified a new causative gene, MBTPS2, in two unrelated patients with OI inherited in X-linked pattern (X-OI). MBTPS2 or site-2 protease (S2P) is a protease that cleaves the transmembrane proteins to activate them acting as transcription factors. To determine pathomechanism of X-OI caused by MBTPS2 mutations, S2P functions were investigated. RNA and protein of S2P were stable. Instead, the defective S2P decreases its cleavage activities. ATF6 and CREB3L1 are target proteins of S2P. We demonstrated decreased a transcriptional activity …
Isoquinoline Alkaloid Composition And Expression Of Biosynthetic Genes In Mechanically Wounded Sacred Lotus, Thitirat Meelaph
Isoquinoline Alkaloid Composition And Expression Of Biosynthetic Genes In Mechanically Wounded Sacred Lotus, Thitirat Meelaph
Chulalongkorn University Theses and Dissertations (Chula ETD)
Isoquinoline alkaloids, a plant secondary metabolites present in many plant species including lotus (Nelumbo nucifera Gaertn.). Lotus leaf contains high amount of bioactive nuciferine and N-nornuciferine. A set of enzyme including NCS, 6OMT and CNMT and WRKY transcription factors (WRKY TFs) has been reported to have corresponding function in the biosynthesis isoquinoline alkaloids. Thus, we performed quantitative analysis on expression level of these corresponing genes and on nuciferine and N-nornuciferine content in lotus leaf using mechanical wounding method. Sequence analysis clearly showed that all the targeted genes possess the conserved region belongs to their protein families. The accumulation of compound …
Effects Of Mitragynine On Morphine And Methamphetamine Addiction In Animal Models Of Addiction, Rittinarong Meepong
Effects Of Mitragynine On Morphine And Methamphetamine Addiction In Animal Models Of Addiction, Rittinarong Meepong
Chulalongkorn University Theses and Dissertations (Chula ETD)
Kratom (Mitragyna speciosa Korth) has long been used in folklore medicine in Southeast Asian countries. It has been used for its analgesic effects and treatment of opioid withdrawal. Mitragynine is a major alkaloid found in the leaves of kratom plant that exerts its effects via activation of opioid receptors and other receptors in the central nervous system. This study aimed to provide evaluation of abuse liability and potential of mitragynine in the treatment for opioids and amphetamine type stimulants addictions. Abuse liability of mitragynine and its effects on morphine and methamphetamine addiction were evaluated by drug discrimination, conditioned place preference …
Bioactive Peptide Derived From Trypsin Hydrolysis Of Unicellular Cyanobacterium Synechococcus Sp., Rutairat Suttisuwan
Bioactive Peptide Derived From Trypsin Hydrolysis Of Unicellular Cyanobacterium Synechococcus Sp., Rutairat Suttisuwan
Chulalongkorn University Theses and Dissertations (Chula ETD)
A free radicals can promote denaturing reactions in many cellular components, which create oxidative stress and it results to damage in body tissue. The free radicals can causing many diseases including heart diseases, Alzheimer, inflammation and cancer. This research aimed to investigate the effect of bioactive peptides derived from Synechococcus sp. cultured for 21 days on antioxidant, anti-inflammation and anticancer activities. The Synechococcus sp. protein hydrolysate was prepared by trypsin digest and purified by ultrafiltration with molecular weight cut off membranes of 10, 5 and 3 kDa. The MW <3 kDa fraction showed the highest NO, ABTS and DPPH radical scavenging activities with IC50 values of 34.51±9.8 µg protein/mL, IC50 11.54±0.3 µg protein/mL and IC50 13.63±0.15 µg protein/mL, respectively. The F2 fraction from gel filtration chromatography showed the strongest NO and ABTS radical scavenging activities with IC50 values of 7.68±0.64 µg protein/mL and 9.74±0.2 µg protein/mL, respectively. The F2 was purified by RP-HPLC to yield four fractions. The 30 – 40 min sub-fraction (F2-4) was selected for further analysis by mass spectroscopy. Five isolated peptides with amino acid sequences of AILQSYSAGKTK; 1,265.69 Da, ALNKTHLIQTK; 1,265.74 Da, LLVHAPVK; 875.55 Da, IPDAHPVK; 875.48 Da and VVVLRDGAVQQLGTPR; 1,706.97 Da were identified. The F2-4 had higher DPPH and NO radical scavenging activity compared to the synthetic peptide. Moreover, AILQSYSAGKTK had the highest ABTS radical scavenging activity. Furthermore, the F2 fraction protected oxidation-induced DNA damage in pBR322, pKS and pUC19 cells. F2 fraction was selected to study anti-inflammatory and anticancer properties. Anti-inflammatory effect, F2 fraction showed no cytotoxicity toward RAW264.7 macrophage cells. RT-PCR and qRT-PCR results showed that F2 reduced gene expression of pro-inflammatory cytokines iNOS, TNF-α, COX-1, COX-2 and IL-6. For anticancer activity, F2 fraction showed high anticancer activities in the human colon cancer cells (SW620) according to cytotoxic activity (MTT assay), with IC50 values of 106.58±21.46 µg protein/mL. The F2 fraction activated the apoptotic pathway in SW620 cells after treatment for 24, 48 and 72 hours. The highest activities of caspases 3, 8 and 9 were observed after treatment for 72 hours. These findings suggested that unicellular cyanobacterium Synechococcus sp. may be used to develop for natural anti-inflammation and natural anticancer drugs.
Antibacterial Agents From Dracaena Cochinchinensis (Lour.) S.C.Chen And Eleutherine Americana (Aubl.) Merr. Ex K. Heyne, Ritbey Ruga
Chulalongkorn University Theses and Dissertations (Chula ETD)
Fifteen compounds including two new anthraquinones (118-119), two naturally occurring new compounds (116-117) and eleven known compounds were successfully isolated from the CH2Cl2 extracts of Dracaena cochinchinensis and Eleutherine americana i.e. dihydrochalcones (5 and 23), pterostilbene (27), homoisoflavanones (15 and 82-84), naphthalenes (85 and 116), pyranonaphthoquinone (86) and anthraquinones (99-100 and 117-119). Among isolated compounds, (+)-eleutherin (86) was the most potent antibacterial agent against Propionibacterium acnes KCCM 41747, Staphylococcus aureus ATCC 25923, Streptococcus sobrinus KCCM 11898, Streptococcus mutans ATCC 25175 and Salmonella typhi ATCC 422 with inhibition zones of 14.7 to 18.0 mm and MIC values of 0.24 to 7.8 …