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Articles 4981 - 5010 of 6471
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Effects Of Non-Albicans Candida Species On Biofilm Formation And Acidogenicity Of Dual-Species Biofilms And On Cariogenic Virulence Gene Expression Of Streptococcus Mutans, Wirunphat Sansanoa
Effects Of Non-Albicans Candida Species On Biofilm Formation And Acidogenicity Of Dual-Species Biofilms And On Cariogenic Virulence Gene Expression Of Streptococcus Mutans, Wirunphat Sansanoa
Chulalongkorn University Theses and Dissertations (Chula ETD)
Early childhood caries is a common oral disease found in children which could affect their general health. Streptococcus mutans is an important cariogenic pathogen since it possesses cariogenic properties, which are acid production, acid tolerance and biofilm formation. Recent studies showed that Candida albicans, which is frequently found together with S. mutans in plaques of children with dental caries, enhances cariogenicity. At the same time, increasing prevalence of non-albicans Candida species (NACs) in children and immune-compromised patients has been reported. Therefore, this study aims to investigate the effects of NACs when co-cultured with S. mutans on cariogenic properties and virulence …
A Feasibility Study Of Diffusion Weighted Imaging And Parametric Response Map Analysis For Treatment Response Prediction In Nasopharyngeal Cancer, Titiya Jirawatwanith
A Feasibility Study Of Diffusion Weighted Imaging And Parametric Response Map Analysis For Treatment Response Prediction In Nasopharyngeal Cancer, Titiya Jirawatwanith
Chulalongkorn University Theses and Dissertations (Chula ETD)
Diffusion-weighted imaging (DWI) is an MRI technique which provides functional information of tissue by detecting microscopic motion of water molecules. The change of apparent diffusion coefficient (ADC) derived from DWI was used as an imaging biomarker for treatment response prediction in cancers. However, it was based on whole-tumor analysis which did not reflect heterogeneity within the tumor. To overcome this limitation, a new method called parametric response map (PRM) analysis was proposed to evaluate response by quantifying voxel-wise changes in ADC. Here we investigated the use of PRM analysis on ADC from DWI as an imaging biomarker for treatment response …
Effect Of Oxidative Stress On Microglial Activation And Iron Accumulation, Rattanakorn Suntawalimp
Effect Of Oxidative Stress On Microglial Activation And Iron Accumulation, Rattanakorn Suntawalimp
Chulalongkorn University Theses and Dissertations (Chula ETD)
Microglia are the glial cell in the central nervous system. They function as resident immune cells in the brain. Microglial activation was an increase in the normal aged brain and also more found in the aged brain with neurodegenerative disease. Activated microglial were found iron accumulation leading to increase ROS generation via the Fenton reaction. Therefore, the increase of microglial activation and iron accumulation is vulnerable to neuronal survival. However, the cause of iron accumulation in the activated microglia still unknown. This study hypothesized that oxidative stress which increases in the aged and neurodegenerative disease brain may cause microglial activation …
Effect Of Canagliflozin On Liver Steatosis In Obese Non Diabetic Patients With Non-Alcoholicfatty Liver Disease: A Double Blind Randomized Controlled Trial, Borwonkhun Tontivuthikul
Effect Of Canagliflozin On Liver Steatosis In Obese Non Diabetic Patients With Non-Alcoholicfatty Liver Disease: A Double Blind Randomized Controlled Trial, Borwonkhun Tontivuthikul
Chulalongkorn University Theses and Dissertations (Chula ETD)
Objective: To demonstrate whether treatment with canagliflozin had an effect on liver steatosis in obese non-diabetic patients with NAFLD compared with the control group Methods: 82 nondiabetic patients with BMI 25-35 kg/m2 underwent 24 weeks of double blind randomized controlled trial with combined canagliflozin 100 mg daily with diet-lifestyle modification or placebo with diet-lifestyle modification (500 calories restriction and moderate intensity aerobic exercise). Primary outcome was the change in Controlled attenuation parameter (CAP) representing hepatic steatosis. Liver stiffness measurement (LSM) representing liver stiffness/fibrosis and other secondary outcomes including changes in body weight, fasting plasma glucose, lipid profile, liver function test …
The Effect Of Parent Involvement-Child Behavioral Management Program Among Children With Adhd, Ubon Wannakit
The Effect Of Parent Involvement-Child Behavioral Management Program Among Children With Adhd, Ubon Wannakit
Chulalongkorn University Theses and Dissertations (Chula ETD)
This quasi-experimental pretest-posttest control group design aims to examine the effectiveness of the Parent Involvement-Child Behavioral Management Program (PICBMP) in reducing symptoms among ADHD children. Sixty-four children aged 6–12 years with ADHD were randomly assigned to either the experimental or control group by using a random number table, consisting of thirty-two participants in each group. The participants in the experimental group participated in the PICBMP and usual care with medication, while those in the control group received the usual care with medication. The PICBMP based on Behaviorally-based treatment (Goodman &Scott, 1997), consists of eight weeks of behavior management which included …
Proximal Caries Detection On Smartphone Display, Napas Lappanakokiat
Proximal Caries Detection On Smartphone Display, Napas Lappanakokiat
Chulalongkorn University Theses and Dissertations (Chula ETD)
The aim of this study was to compare diagnostic accuracy in proximal caries detection between bitewing radiographs exported from PACS software and taken with a smartphone viewed in a smartphone display. A total of 200 proximal surfaces from digital bitewing radiographs were included in this study. Images of all radiographs were captured from a medical-grade and a common display by an iPhone 8 Plus and stored as JPEG files. Exported DICOM files were converted into JPEG format and transferred to the smartphone used for image capturing. Each proximal surface was rated by 7 observers with 5-point-scale. Weighted kappa test was …
The Efficacy Of Vitamin C Oral Supplement On Wound Healing After Tooth Extraction, Nanthanut Pisalsitsakul
The Efficacy Of Vitamin C Oral Supplement On Wound Healing After Tooth Extraction, Nanthanut Pisalsitsakul
Chulalongkorn University Theses and Dissertations (Chula ETD)
Objectives: The purpose of this study was to evaluate the effect and proper dosage of oral vitamin C supplement on post-extraction wound healing. Methods: This study was a split mouth, double-blind, randomized-controlled clinical trial of 42 patients who underwent symmetric bilateral non-infected premolars extraction. The patients were randomly divided into 3 groups (14 patients for each group); 1.placebo vs vitamin C 600 mg/d, 2.placebo vs vitamin C 1,500 mg/d and 3.vitamin C 600 mg/d vs vitamin C 1,500 mg/d. Each group was prescribed placebo and/or vitamin C three times a day for 10 days after each tooth extraction. The assessment …
Use Of Digital Panoramic Radiographs As A Screening Tool For Diagnosis Of Osteoporosis In Thai Postmenopausal Women, Net-Nada Chongruangsri
Use Of Digital Panoramic Radiographs As A Screening Tool For Diagnosis Of Osteoporosis In Thai Postmenopausal Women, Net-Nada Chongruangsri
Chulalongkorn University Theses and Dissertations (Chula ETD)
Purpose: To investigate the correlation between panoramic radiographic indices and osteoporosis, and to determine whether digital panoramic radiographs could be used as a screening tool for the diagnosis of osteoporosis in Thai postmenopausal women. Materials and Methods: A cross-sectional study of sixty Thai postmenopausal women with and without osteoporosis. The subjects were divided into three groups based on diagnosis by dual-energy X-ray absorptiometry (DXA): normal, osteopenia, and osteoporosis equally in each group. The panoramic radiographic indices measured were mental index (MI) and mandibular cortical index (MCI). Pearson’s correlation test was performed to analyze any correlation between MI, MCI, and BMD …
Production Of Bio-Jet Fuel From Palm Fatty Acid Distillate Over Bi-Functional Copd/Hzsm-12 Catalysts, Jarujit Chaowichitra
Production Of Bio-Jet Fuel From Palm Fatty Acid Distillate Over Bi-Functional Copd/Hzsm-12 Catalysts, Jarujit Chaowichitra
Chulalongkorn University Theses and Dissertations (Chula ETD)
Jet fuel is one of the major products from a petroleum refinery, its combustion emissions contribute to global warming. Therefore, bio-jet fuel is introduced to solve this problem. Palm fatty acid distillate (PFAD) is an interesting feedstock for bio-jet fuel production via three reactions including deoxygenation, hydrocracking, and isomerization. In this study, the monometallic (Co and Pd) and bimetallic (CoPd and NiPd) supported on HZSM-12 zeolite with various metal loadings and bimetallic CoPd with various zeolite supports (HZSM-12, HZSM-5, and HY) were investigated in a continuous flow trickle bed reactor. The presence of bimetallic CoPd on HZSM-12 zeolite support enhanced …
The Study Of Sulfur Compounds For Oils Fingerprinting Application, Titiparn Prasantongkolmol
The Study Of Sulfur Compounds For Oils Fingerprinting Application, Titiparn Prasantongkolmol
Chulalongkorn University Theses and Dissertations (Chula ETD)
Oil spills are one of the serious problems to marine and coastal environment worldwide. Besides the known incidents, a number of oil spills are unknown, which may come from both accident and unauthorized drainage. Moreover, the spills cause tarball to arise on the beach and the origin of tarballs are still subtle. Thus, the development of oil fingerprinting is important in order to identify the origin of oil spills and tarballs. Generally, the current standard technique is using GCxGC-TOFMS to analyze the biomarker compounds in oil such as hopane groups. However, this technique is expensive and requires long time for …
Continuous Synthesis Of Biodiesel And Glycerol Ether Using Homogeneous And Heterogeneous Catalysts, Natta Rattanapanya
Continuous Synthesis Of Biodiesel And Glycerol Ether Using Homogeneous And Heterogeneous Catalysts, Natta Rattanapanya
Chulalongkorn University Theses and Dissertations (Chula ETD)
In our study, a mixture of p-toluenesulfonic acid and sulfuric acid (TsOH-H2SO4) as a catalyst showed a good performance in transesterification of palm oil (PO) with methanol and etherification of biodiesel with isobutylene. For biodiesel production, the catalyst noticeably accelerated the reaction faster than TsOH and H2SO4 alone and also gave up to 99.8% of the conversion. Etherification of biodiesel with isobutylene in the etherification process using isobutylene/glycerol molar ratio 6:1 at 70°C, in the period of 5 h reaction time, gave high selectivity to produce DTBG and TTBG (81.4%). Furthermore, the catalyst can be reused for 6 cycles of …
Electrospun Nylon 6,6 Nanofibers Functionalized With Β-Cyclodextrin As Solid Phase Extraction Sorbent For Determination Of Parabens, Tassanee Kuakul
Electrospun Nylon 6,6 Nanofibers Functionalized With Β-Cyclodextrin As Solid Phase Extraction Sorbent For Determination Of Parabens, Tassanee Kuakul
Chulalongkorn University Theses and Dissertations (Chula ETD)
In this study, electrospun nanofibers of nylon 6,6 functionalized with β-cyclodextrin (nylon 6,6/β-CD) were fabricated and applied as a sorbent in solid-phase extraction (SPE) for determination of parabens in water. The electrospinning parameters such as polymer concentration, nylon 6,6/β-CD ratio, and electric field strength were investigated in order to obtain uniform nanofibers. The morphology and physical properties of obtained fibers were characterized by scanning electron microscope (SEM), Fourier transforms infrared spectroscopy (FTIR), and BET surface area analysis. The electrospun nylon 6,6/β-CD nanofibers were achieved with diameter in the range of 120 - 150 nm. The additional of β-CD slightly increased …
In Vitro Hepatic Metabolism Of Curcumin Diethyl Disuccinate In Different Animal Species, Ponsiree Jithavech
In Vitro Hepatic Metabolism Of Curcumin Diethyl Disuccinate In Different Animal Species, Ponsiree Jithavech
Chulalongkorn University Theses and Dissertations (Chula ETD)
Curcumin diethyl disuccinate (CDD) is an ester prodrug of the active compound, curcumin. CDD has been improved chemical stability and intestinal permeability more than curcumin. CDD demonstrated biological activity higher than curcumin. The aim of this study is to investigate a suitability of using liver S9 fraction (LS9) in drug metabolism profiling as function of animal sources of enzyme regarding possible translation of in vitro outcome to clinical study. The metabolites, in vitro kinetics and esterase enzyme involved in CDD hydrolysis by LS9 of human, monkey, dog and rat ware determined. The high-performance liquid chromatography conjugated with high-resolution mass spectrometry-based …
Development Of Mucoadhesive Biloniosome Nanoparticles For Enhanced Gastrointestinal Absorption Of Cordycepin, Warut Kengkittipat
Development Of Mucoadhesive Biloniosome Nanoparticles For Enhanced Gastrointestinal Absorption Of Cordycepin, Warut Kengkittipat
Chulalongkorn University Theses and Dissertations (Chula ETD)
Cordycepin, an adenosine analogue, has displayed numerous pharmacological and therapeutic implications including enhanced apoptosis and inhibition of proliferation of cancer cells, antioxidant property and improving sexual activity. However, oral administration of cordycepin has limited practical use due to its poor bioavailability in the intestine. Here, we developed a novel hybrid nanoparticle system for effective delivery of cordycepin in the gastrointestinal tract by using the bile salt-based liposome/niosome hybrid system (designated as biloniosome). In addition, mucoadhesive chitosan biopolymer was coated on the biloniosome nanoparticles to improve the mucoadhesive properties thereby enhancing the drug efficiency. Cordycepin-encapsulated biloniosome nanoparticles were synthesized by solvent …
Expression Of Human Dentin Matrix Protein 1 In Escherichia Coli And Nicotiana Benthamiana And Their Effect On Proliferation And Osteogenic Differentiation Of Human Periodontal Ligament Stem Cells, Aktsar Roskiana Ahmad
Expression Of Human Dentin Matrix Protein 1 In Escherichia Coli And Nicotiana Benthamiana And Their Effect On Proliferation And Osteogenic Differentiation Of Human Periodontal Ligament Stem Cells, Aktsar Roskiana Ahmad
Chulalongkorn University Theses and Dissertations (Chula ETD)
This study aimed to express recombinant protein human Dentin Matrix Protein 1 (hDMP1) in Escherichia coli and Nicotiana benthamiana and investigated the osteogenic differentiation effects in human Periodontal Ligament Stem Cells (hPDLSCs). The gene encoding hDMP1 was ligated into the pET22b and pBY2R2e expression vectors. Recombinant hDMP1 protein has been productively expressed in E. coli and N. benthamiana. E. coli-produced hDMP1 resulted in 60 kDa and 100, and the optimal condition to produce highly expression was demonstrated at 0.5 mM IPTG and incubation at 37oC for 6 hr. N. benthamiana-produced hDMP1 protein showed in a 100 kDa that indicated the …
Inhibitory Effects Of Compounds Isolated From Ethyl Acetate Extracts Of Glycosmis Parva Branches And Leaves On Lps-Induced Raw 264.7 Macrophage Activation, Chanyanuch Laprasert
Inhibitory Effects Of Compounds Isolated From Ethyl Acetate Extracts Of Glycosmis Parva Branches And Leaves On Lps-Induced Raw 264.7 Macrophage Activation, Chanyanuch Laprasert
Chulalongkorn University Theses and Dissertations (Chula ETD)
Macrophages play major roles to produce several inflammatory mediators in many chronic inflammatory diseases. Current anti-inflammatory drugs target these mediators to alleviate inflammation symptoms. Searching for new anti-inflammatory agents is always needed due to problems from currently used anti-inflammatory drugs. This study aimed to investigate the anti-inflammatory potential of three main compounds, arborinine, N-methylatalaphylline and S-deoxydihydroglyparvin (DDGP), isolated from Glycosmis parva leaves and branches on lipopolysaccharide (LPS)-activated macrophage RAW 264.7 cells. Only DDGP demonstrated a potent inhibitor of LPS-activated macrophages. It inhibited expression of inducible nitric oxide synthase and decreased nitric oxide production with IC50 3.47 ± 0.1 µM. It …
Anti-Cancer Effects Of Renieramycin T On Human Non-Small Cell Lung Cancer Cells, Korrakod Petsri
Anti-Cancer Effects Of Renieramycin T On Human Non-Small Cell Lung Cancer Cells, Korrakod Petsri
Chulalongkorn University Theses and Dissertations (Chula ETD)
Mcl-1 targeted therapy is promising strategy for lung cancer treatment since it is contributed to aggressive lung cancer and chemotherapeutic resistance. At present, many compounds targeting on Mcl-1 are currently under different clinical stages of development for cancer treatment. Herein, we report the Mcl-1 targeting activity of renieramycin T (RT), an alkaloid isolated from the blue sponge Xestospongia sp. RT was shown to target on Mcl-1 through ubiquitin-proteasomal degradation in lung cancer cells including chemotherapeutic resistant primary lung cancer cells. Additionally, RT also affects the activation of p53 and decrease of Bcl-2 which can lead to apoptosis of cancer cells. …
The Incidence Of Antibiotic-Resistant Staphylococcus Spp. Colonization And Infection In Children And Adults With Atopic Dermatitis, Matchima Laowansiri
The Incidence Of Antibiotic-Resistant Staphylococcus Spp. Colonization And Infection In Children And Adults With Atopic Dermatitis, Matchima Laowansiri
Chulalongkorn University Theses and Dissertations (Chula ETD)
Atopic dermatitis (AD) is an inflammatory skin disease characterized by chronic and recurrent eczematous rash. AD occurs in both children and adults, but children are more affected. An important aggravating factor is caused by Staphylococcus aureus (S. aureus) infection. Therefore, topical (beta-lactam, macrolides, clindamycin) and systemic (mupirocin and fusidic acid) antibiotics are essential for treatment of AD. However, frequent use of antibiotics results in an increase in the incidence of antibiotic-resistant S. aureus. Methicillin resistance S. aureus (MRSA) becomes a problem in the treatment of skin diseases. It’s found that the change from penicillin binding protein (PBP) to PBP2a with …
Factors Associated With Utilization Of Maternal Health Services In Myanmar, Myint Kyaw Swar
Factors Associated With Utilization Of Maternal Health Services In Myanmar, Myint Kyaw Swar
Chulalongkorn University Theses and Dissertations (Chula ETD)
One of the significant causes of maternal deaths during pregnancy is low utilization of maternal health services, particularly in limited-resource settings. Proper utilization of these services can help reduce the rate of maternal mortality. Utilization of maternal health services is currently being promoted in Myanmar, however, the factors which influence on the use of these services have not been explored broadly. This present study aimed to explore the levels and patterns of utilization of maternal health services among Myanmar mothers, as well as the factors which could influence their utilization including general characteristics, mass media exposure, and woman decision making …
Pelletization Of Nickel Catalyst For Hydrogen Production From Glycerol Steam Reforming, Jinnawat Saengsawang
Pelletization Of Nickel Catalyst For Hydrogen Production From Glycerol Steam Reforming, Jinnawat Saengsawang
Chulalongkorn University Theses and Dissertations (Chula ETD)
This work investigated the pelletization of Ni/Al2O3 catalyst for hydrogen production from glycerol steam reforming (GSR). Effects of preparation methods, binders, and pellet shapes (cylindrical/spherical) on the catalyst's physicochemical properties and catalytic performance in a fixed-bed reactor were considered. Freshly prepared catalyst was characterized with XRD, N2 adsorption-desorption, SEM/EDX, H2-TPR, and H2-chemisorption. The crushing strength of the catalyst pellets was also evaluated by a hydraulic press. GSR was carried out at a water to glycerol molar ratio of 9: 1, a total liquid flow rate of 0.04 ml/min, 600 ºC, and 1 atm. Ni/Al2O3 catalyst pellets showed superior catalytic performance …
Bacterial Cellulose/Alginate/Gelatin Composite Films For Wound Dressing, Nadda Chiaoprakobkij
Bacterial Cellulose/Alginate/Gelatin Composite Films For Wound Dressing, Nadda Chiaoprakobkij
Chulalongkorn University Theses and Dissertations (Chula ETD)
This research aimed to develop the blended bacterial cellulose (BC)/ alginate (A)/ gelatin (G) incorporating with curcumin (C) for wound dressing application. Fourier transform infrared (FT-IR) spectroscopy showed that the interaction among bacterial cellulose/alginate/gelatin curcumin molecules were only physical but not chemical intermolecular level. Water vapor and oxygen permeabilities of the fabricated films were close to commercially avaialbe wound dressings. Curcumin incorporation lowered fluid absorption ability, water vapor and oxygen permeabilities. In vitro culture of human keratinocytes, and human gingival fibroblasts evidenced that the fabricated composite films could promote cell proliferation. Curcumin-loaded films showed anticancer activity against human oral cancer …
Reinforcing Natural Rubber Latex Foam With Nano And Micro Cellulose Fibers Via Dunlop Method, Sirilak Phomrak
Reinforcing Natural Rubber Latex Foam With Nano And Micro Cellulose Fibers Via Dunlop Method, Sirilak Phomrak
Chulalongkorn University Theses and Dissertations (Chula ETD)
Natural rubber latex foam (NRLF) was reinforced with micro- and nano-cellulose at a loading content of 5-20 parts per hundred of rubber (phr) via the Dunlop process. Cellulose powder from eucalyptus pulp and bacterial cellulose (BC) was used as a microcellulose (MC) and nanocellulose (NC) reinforcing agent, respectively. NRLF, NRLF-MC, and NRLF-NC exhibited interconnected macroporous structures with a high porosity and a low-density. The forms contained pores with sizes in a range of 10-500 µm. As compared to MC, NC had a better dispersion inside the NRLF matrix and showed a higher adhesion to the NRLF matrix, resulting in a …
Prediction Of Catalyst Bed Density And Simulation Of Glycerol Steam Reformer For Hydrogen Production, Vorathorn Charoensuk
Prediction Of Catalyst Bed Density And Simulation Of Glycerol Steam Reformer For Hydrogen Production, Vorathorn Charoensuk
Chulalongkorn University Theses and Dissertations (Chula ETD)
A simple method was proposed to generate a rigid model and to predict the packing density of catalyst using Blender software. It is a free and open-source 3D creation suite animating catalyst pellets freely falling into a container. The effects of different catalyst shapes (sphere and cylinder) and sizes (2–5 mm) on the packing density of catalyst bed were investigated. It was found that the void fraction decreased with decreasing catalyst pellet diameter for all reactor diameters (18-50 mm). Moreover, the void fraction decreased with increasing reactor size for all catalyst shapes and sizes. The results also showed that the …
Development Of Reimbursement Model Forhome-Based Chemotherapy In Thailand, Nattanichcha Kulthanachairojana
Development Of Reimbursement Model Forhome-Based Chemotherapy In Thailand, Nattanichcha Kulthanachairojana
Chulalongkorn University Theses and Dissertations (Chula ETD)
The objectives of the study were (1) to review on HC reimbursement model in other countries (2) to conduct economic evaluation comparing HC with inpatient chemotherapy and hospital-based chemotherapy treatment (IP) from a societal perspective (3) to develop national models and financing and reimbursing of HC services in Thailand. Literature review and semi-structured interviews as the mixed methods had been applied for data collection. The characteristic of HC and reimbursement policies for public payer and service delivery were obtained from eight countries: Australia, Canada (Ontario), England, France, Japan, Korea, Singapore and the United States (US) (Medicare Part B). A cost-utility …
Mixture Of Medium Chain Fatty Acids For Protein Precipitation In Purification Process Of Equine Anti-Rabies Immunoglobulin As F(Ab′)2, Sirisak Sathorn
Mixture Of Medium Chain Fatty Acids For Protein Precipitation In Purification Process Of Equine Anti-Rabies Immunoglobulin As F(Ab′)2, Sirisak Sathorn
Chulalongkorn University Theses and Dissertations (Chula ETD)
Besides the widely used caprylic acid (C8) as protein precipitating agent, capric acid (C10) and lauric acid (C12) were investigated as alternative medium chain fatty acids (MCFAs) for their capabilities on protein purification. The optimization process conducted with normal bovine plasma as a model antibody demonstrated the essential effect of temperature during filtration to significantly reduce the content of albumin, a contaminant marker, compared with filtration at room temperature (p<0.05). From SDS-PAGE analysis, the optimal concentrations of 3% C8 and 5% C10 increased IgG purity from 30.2% to 94.6% and 80.4%, respectively. Interestingly, precipitation by C10 provided the lowest turbidity of filtrates. Purification by C12 showed unsatisfied results that albumin content was minutely decreased when increasing C12 concentration. C8 and C10 were then variously combined into 2 levels of 3% and 5% binary mixture for plasma purification. The synergist effect of C8 and C10 mixtures was clearly demonstrated in albumin elimination that the purity of fractionates were increased to 95%. Moreover, binary mixtures with higher concentration of C10 improved the filtration velocity in plasma model. Selected C8:C10 mixtures were then applied for both ERIG-IgG and ERIG-F(ab′)2 purification. SDS-PAGE analysis confirmed the absence of albumin protein in all purified samples. For ERIG-IgG purification, C8:C10 mixture at ratio 1.5:3.5 demonstrated the best in homogeneity with high recovery rate of bioactive antibody, while the mixtures at 2.25:0.75 showed the best purification capabilities to obtain highly purified ERIG-F(ab′)2. Furthermore, the purification by C8:C10 mixtures displayed superior process characteristic than the commercially non-chromatographic technique for ERIGF(ab′)2 purification in terms of antibody recovery, purity and lower in aggregate formation. The novelty on beneficial use of MCFA mixture as protein precipitating agent for antibody purification has been demonstrated.
Development Of Spray Dried Encapsulated Curcumin Powder As Functional Food Ingredient, Alwani Hamad
Development Of Spray Dried Encapsulated Curcumin Powder As Functional Food Ingredient, Alwani Hamad
Chulalongkorn University Theses and Dissertations (Chula ETD)
This study investigated the development of the spray-dried encapsulated curcumin powder from an oil-water-emulsion with better functional properties and evaluated its stability characters during storage and reconstitution. This research was conducted in four parts. The first part was the selection and optimization of curcumin solubility in edible oil. It was found that MCT oil was the best carrier oil with curcumin solubility of 17.01 mg/mL, which was achieved by heating at 80oC for 10 min. The curcumin solubility in MCT oil was further applied to nanoemulsion and conventional emulsion, in which the conventional one showed shorter stability...
Esterification Of Lignin As Carbon Fiber Production Precursor, Jarudech Rakphong
Esterification Of Lignin As Carbon Fiber Production Precursor, Jarudech Rakphong
Chulalongkorn University Theses and Dissertations (Chula ETD)
This research investigated the optimized condition (i.e., esterifying agent, reaction time, reaction temperature) to modify lignin for utilization as a precursor in carbon fiber production. The lignin was successfully modified by esterification reaction in dimethylformamide solvent under different reaction temperatures and times using different types of fatty acid chloride including butyryl chloride, octanoyl chloride, and lauroyl chloride as an esterifying agent. The effects of fatty acid chloride chain length and degree of substitution on the chemical structure, functional groups, morphology, crystallinity, thermal properties, and solubility of esterified-lignin were studied. The chemical structure of the obtained product was characterized by FTIR …
Effects Of Hydrogen Synthesis Inhibition On Changes Of Bioproduct Contents In Cyanobacterium Synechocystis Sp. Pcc 6803, Nannaphat Sukkasam
Effects Of Hydrogen Synthesis Inhibition On Changes Of Bioproduct Contents In Cyanobacterium Synechocystis Sp. Pcc 6803, Nannaphat Sukkasam
Chulalongkorn University Theses and Dissertations (Chula ETD)
Cyanobacteria have an ability to convert carbon dioxide and solar light to bioproducts. Synechocystis sp. PCC 6803 is the laboratory model strain of cyanobacteria with known metabolism and biochemistry. NADPH is one of the essential electron carriers and coenzymes required for various cellular metabolism including the production of bioproducts. [NiFe]-Hydrogenase consumes NADPH to produce hydrogen gas which considerably the loss of metabolic energy. Thus, the elimination of [NiFe]-hydrogenase would increase NADPH level in the cell and might increase the accumulation of bioproducts. In this work, hoxY and hoxH genes (encoding the two catalysis subunits of [NiFe]-hydrogenase) were inactivated. The inactivation …
Effect Of Poly(Lactic Acid) On Properties Of Poly(Butylene Succinate)/Poly(Butylene Succinate-Co-Adipate)/ Poly(Lactic Acid) Blend Films, Narumon Puekpoonpoal
Effect Of Poly(Lactic Acid) On Properties Of Poly(Butylene Succinate)/Poly(Butylene Succinate-Co-Adipate)/ Poly(Lactic Acid) Blend Films, Narumon Puekpoonpoal
Chulalongkorn University Theses and Dissertations (Chula ETD)
Nowadays, biodegradable plastics have drawn a great deal of attention because of plastic pollution. The major drawback of their mechanical properties brought to a limitation of neat biodegradable resins for flexible packaging applications. In this study, binary and ternary blends of poly(butylene succinate-co-adipate) (PBSA) with poly(butylene succinate) (PBS) and poly(lactic acid) (PLA) were melt compounded and successfully prepared into films via blown film line. The aim of this study was to investigate the influence of incorporating PLA including of high molecular weights PLA (Hi-PLA) and low molecular weights (Lo-PLA) into PBSA/PBS blends on processability, phase morphology, mechanical and physical properties. …
Chemical Constituents From The Twigs And Branches Of Garcinia Schomburgkiana, Nindyashinta Darnasmara
Chemical Constituents From The Twigs And Branches Of Garcinia Schomburgkiana, Nindyashinta Darnasmara
Chulalongkorn University Theses and Dissertations (Chula ETD)
The phytochemical investigation from the dichloromethane extract of the twigs and branches of Garcinia schomburgkiana led to the isolation of fourteen known compounds, including 2'-hydroxydihydrochalcone (1), 1-(2'-6'-dihydroxyphenyl)-3-phenyl-1-propanone (2), cudratricusxanthone K (3), pancixanthone A (4), euxanthone (5), 1,4,5-trihydroxyxanthone (6), osajaxanthone (7), 1,2,7-trihydroxyxanthone (8), 1,3,7-trihydroxy-2,4-diprenylxanthone (9), 1,5-dihydroxyxanthone (10), 7-epiclusianone (11), oblongifolin C (12), clusiacitran B (13), and aucuparin (14). Among all isolated compounds, 2'-hydroxydihydrochalcone, 1-(2'-6'-dihydroxyphenyl)-3-phenyl-1-propanone, and cudratricusxanthone K were isolated from genus Garcinia for the first time. Moreover, 7-epiclusianone, pancixanthone A, 1,4,5-trihydroxyxanthone, osajaxanthone, 1,2,7-trihydroxyxanthone, and 1,3,7-trihydroxy-2,4-diprenylxanthone discovered in G. schomburgkiana for the first time. The structures of all isolated compounds were characterized …