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Estimation Of Wave Energy From Wind Velocity, Jonathan Blackledge, Eugene Coyle, Derek Kearney, Ronan Mcguirk, Brian Norton Jan 2013

Estimation Of Wave Energy From Wind Velocity, Jonathan Blackledge, Eugene Coyle, Derek Kearney, Ronan Mcguirk, Brian Norton

Articles

The aim of this paper is to report on a possible correlation between the Levy index for wind velocity and the mean Energy Density of sea surface waves in the same location. The result is based on data obtained from 6 buoys located around the coast of Ireland and maintained by the Marine Institute of Ireland and a further 144 buoys located at various locations off the coast of the United States of America and maintained by the National Data Buoy Centre. These buoys provide historical data on the wind velocity, wave height and wave period as well as other …


Financial Forecasting Using The Kolmogorov-Feller Equation, Jonathan Blackledge, Marc Lamphiere, Kieran Murphy, Shaun Overton Jan 2013

Financial Forecasting Using The Kolmogorov-Feller Equation, Jonathan Blackledge, Marc Lamphiere, Kieran Murphy, Shaun Overton

Articles

An approach to analysing a financial time series using the Kolmogorov-Feller Equation is considered, in particular, the Generalised Kolmogorov-Feller Equation (GKFE) subject to variations in the Stochastic Volatility. Using the Mittag-Leffler memory function, we derive an expression for the Impulse Response Function associated with a short time window of data which is then used to derive an algorithm for computing a new index using a standard moving window process. It is shown that application of this index to financial time series, subject to a low volatility condition, correlates with the start, direction and end of a trend depending on the …


The Fractional Schrodinger-Klein-Gordon Equation And Intermediate Relativism, Jonathan Blackledge, Bazar Babajanov Jan 2013

The Fractional Schrodinger-Klein-Gordon Equation And Intermediate Relativism, Jonathan Blackledge, Bazar Babajanov

Articles

By considering a random walk model compounded in Einstein's evolution equation, we show that both the classical Schrodinger and Klein-Gordon equations can be viewed a s consequence of introducing a memory function.

The purpose of this is to derive a wave equaiton that, on a phenomenological basis at least, describes the transitional characteristics of wave functions for spin-less particles that may exist in the intermediate of "semi relativistic regime.


A Review Of Existing Microgrid Architectures, Lubna Mariam, Malabika Basu, Michael Conlon Jan 2013

A Review Of Existing Microgrid Architectures, Lubna Mariam, Malabika Basu, Michael Conlon

Articles

The future electricity network must be flexible, accessible, reliable, and economic according to the worldwide smartgrid initiative. This is also echoed by the Sustainable Energy Authority of Ireland (SEAI) and European Electricity Grid Initiative (EEGI). In order to facilitate these objectives and to reduce green house gas (GHG) emission, research on various configurations of microgrid system is gaining importance, particularly with high penetration of renewable energy sources. Depending on the resource availability, geographical locations, load demand, and existing electrical transmission and distribution system, uG can be either connected to 6the grid or can work in an autonomous mode. Storage can …


Selection Of Design Parameters To Reduce The Zero-Sequence Circulating Current Flow In Parallel Operation Of Dc Linked Multiple Shunt Apf Units, Shafiuzzaman Khadem, Malabika Basu, Michael Conlon Jan 2013

Selection Of Design Parameters To Reduce The Zero-Sequence Circulating Current Flow In Parallel Operation Of Dc Linked Multiple Shunt Apf Units, Shafiuzzaman Khadem, Malabika Basu, Michael Conlon

Articles

Capacity enhancement and operational flexibility are two of the important limitations of the centralized shunt APF unit. These limitations can be conquered by the operation of multiple APF units in parallel and connected back to back by a common DC link capacitor. In that case, a circulating current (CC) flows within the APF units. This CC flow becomes out of control when the APF units operate in hysteresis based current controlled mode. One of the difficulties of this CC flow control or reduction is the variable switching frequency of the APF units. In this paper the model for CC flow …


Correspondence: Comparing Led Luminaires, James Duff Jan 2013

Correspondence: Comparing Led Luminaires, James Duff

Articles

A recent editorial within Lighting Research and Technology 1 suggests the need for ‘a simple set of questions to ask the LED supplier’. This article will present such a set of questions, but also, and perhaps more importantly, a set of responses that might be considered appropriate. The information used to form these questions and answers has been drawn from a number of useful resources 2-7, interaction with various lighting professionals and through the authors own experience. The purpose of these questions is to generate discussion. The author understands the questions posed can be improved upon and it is hoped …


Biaxial Order Parameter In The Homologous Series Of Orthogonal Bent-Core Smectic Liquid Crystals, S. Sreenilayam, Yuri Panarin, J. K. Vij, M. Osipov, A. Lehmann, C. Tschierske Jan 2013

Biaxial Order Parameter In The Homologous Series Of Orthogonal Bent-Core Smectic Liquid Crystals, S. Sreenilayam, Yuri Panarin, J. K. Vij, M. Osipov, A. Lehmann, C. Tschierske

Articles

The fundamental parameter of the uniaxial liquid crystalline state that governs nearly all of its physical properties is the primary orientational order parameter (S) for the long axes of molecules with respect to the director. The biaxial liquid crystals (LCs) possess biaxial order parameters depending on the phase symmetry of the system. In this paper we show that in the first approximation a biaxial orthogonal smectic phase can be described by two primary order parameters: S for the long axes and C for the ordering of the short axes of molecules. The temperature dependencies of S and C are obtained …


Finite Element Analysis Of Balloon-Expandable Coronary Stent Deployment: Influence Of Angioplasty Balloon Configuration, David Martin, Fergal Boyle Jan 2013

Finite Element Analysis Of Balloon-Expandable Coronary Stent Deployment: Influence Of Angioplasty Balloon Configuration, David Martin, Fergal Boyle

Articles

Today, the majority of coronary stents are balloon-expandable and are deployed using a balloon-tipped catheter. To improve deliverability, the membrane of the angioplasty balloon is typically folded about the catheter in a pleated configuration. As such, the deployment of the angioplasty balloon is governed by the material properties of the balloon membrane, its folded configuration and its attachment to the catheter. Despite this observation, however, an optimum strategy for modelling the configuration of the angioplasty balloon in finite element studies of coronary stent deployment has not been identified, and idealised models of the angioplasty balloon are commonly employed in the …


Improving Tribological Properties Of Cast Al-Si Alloys Through Application Of Wear-Resistant Thermal Spray Coatings, David Culliton, Tony Betts, Sandra Carvalho, David Kennedy Jan 2013

Improving Tribological Properties Of Cast Al-Si Alloys Through Application Of Wear-Resistant Thermal Spray Coatings, David Culliton, Tony Betts, Sandra Carvalho, David Kennedy

Articles

Flame Spray Thermal Spray coatings are low-cost, high-wear surface-treatment technologies. However, little has been reported on their potential effects on cast automotive aluminum alloys. The aim of this research was to investigate the tribological properties of as-sprayed NiCrBSi and WC/12Co Flame Spray coatings applied to two cast aluminum alloys: high-copper LM24 (AlSi8Cu3Fe), and low-copper LM25 (AlSi7Mg). Potential interactions between the mechanical properties of the substrate and the deposited coatings were deemed to be significant. Microstructural, microhardness, friction, and wear (pin-on-disk, microabrasion, Taber abrasion, etc.) results are reported, and the performance differences between coatings on the different substrates were noted. The …


Issues Associated With Planning, Implementing, Managing And Operating Public Transport Projects In Ireland., Roisin Byrne-Murray, Mike Murphy, Aoife Ahern Jan 2013

Issues Associated With Planning, Implementing, Managing And Operating Public Transport Projects In Ireland., Roisin Byrne-Murray, Mike Murphy, Aoife Ahern

Articles

Whilst considering the context to which public transport is planned in Ireland and internationally, the research has been concerned with studying the methodology and the roles of key stakeholders for the planning, implementation, management and operation of public transport projects in order to gauge the efficiency and suitability of the Irish planning process in relation to our international counterparts The paper will present principal findings from structured interviews undertaken with key stakeholders involved in the Irish process and will provide a basis for debating the issues raised as a consequence of these interviews.. Key issues raised include: 1) the possible …


Impact Of Intermetallic Precipitates On The Tribological And/Or Corrosion Performance Of Cast Aluminium Alloys: A Short Review, David Culliton, Tony Betts, David Kennedy Jan 2013

Impact Of Intermetallic Precipitates On The Tribological And/Or Corrosion Performance Of Cast Aluminium Alloys: A Short Review, David Culliton, Tony Betts, David Kennedy

Articles

The role of various intermetallic precipitates (IMP), or secondary phase particles, in governing the wear and corrosion performance characteristics of cast aluminium alloys is outlined in this brief review. Such alloys are especially important in transport applications where their low weight, low cost and recyclability make them very attractive. However alloy wear and/or corrosion behaviour often limit their industrial application and more work needs to done to extend their use into other areas. Careful control of IMP nucleation and growth rates may be beneficial, especially in alloys exposed to corrosive environments. Silicon, copper and magnesium are all important elements for …


Lumped Time-Delay Compensation Scheme For Coding Synchronization In The Nonlinear Spectral Quantization-Based All-Optical Analog-To-Digital Conversion, Zhe Khang, Jinhui Yuan, Qiang Wu, Tao Wang, Sha Li, Chongxiu Y, Gerald Farrell Jan 2013

Lumped Time-Delay Compensation Scheme For Coding Synchronization In The Nonlinear Spectral Quantization-Based All-Optical Analog-To-Digital Conversion, Zhe Khang, Jinhui Yuan, Qiang Wu, Tao Wang, Sha Li, Chongxiu Y, Gerald Farrell

Articles

this paper, we propose a novel lumped time-delay compensation scheme for the all-optical analog-to-digital conversion based on soliton self-frequency shift and optical interconnection techniques. By inserting a segment of negative dispersion fiber between the quantization and the coding module, the time delay of different quantized pulses can be accurately compensated with a simple structure compared to the multiple time-delay lines. The simulation results show that the coding pulses can be well synchronized using a span of fiber, with the flattened negative dispersion within the wavelength range of 1558-1620 nm. In addition, the problems of pulse broadening and time error are …


Apoptosis Is Signalled Early By Low Doses Of Ionizing Radiation In A Radiation-Induced Bystander Effect, Hayley Furlong, Carmel Mothersill, Fiona Lyng, Orla Howe Jan 2013

Apoptosis Is Signalled Early By Low Doses Of Ionizing Radiation In A Radiation-Induced Bystander Effect, Hayley Furlong, Carmel Mothersill, Fiona Lyng, Orla Howe

Articles

It is known that ionising radiation (IR) induces a complex signalling apoptotic cascade post-exposure to low doses ultimately to remove damaged cells from a population, specifically via the intrinsic pathway. Therefore, it was hypothesised that bystander reporter cells may initiate a similar apoptotic response if exposed to low doses of IR (0.05 Gy and 0.5 Gy) and compared to directly irradiated cells. Key apoptotic genes were selected according to their role in the apoptotic cascade; tumour suppressor gene TP53, pro-apoptotic Bax and anti-apoptotic Bcl2, pro-apoptotic JNK and anti-apoptotic ERK, initiator caspase 2 and 9 and effector caspase 3, 6 and …


Mothers Who Formula Feed: Their Practices, Support Needs And Factors Influencing Their Infant Feeding Decision., Roslyn Tarrant, Margaret Sheridan-Pereira, Roberta A. Mccarthy, Katherine M. Younger, John M. Kearney Jan 2013

Mothers Who Formula Feed: Their Practices, Support Needs And Factors Influencing Their Infant Feeding Decision., Roslyn Tarrant, Margaret Sheridan-Pereira, Roberta A. Mccarthy, Katherine M. Younger, John M. Kearney

Articles

The majority of mothers in Ireland provide formula milk to their infants during the initial weeks postpartum; however, data are lacking on their formula feeding practices and support needs. This prospective Dublin-based observational study, which included 450 eligible mother-term infant pairs recruited and followed up to 6 months postpartum, aimed to advance our understanding of maternal formula feeding practices, their reasons for deciding to formula feed, sources of feeding information and perceived support needs; insights into infant formula milk consumption patterns in relation to current feeding guidelines are also provided. In summary, the vast majority of infants at 6 weeks …


Epigenetic Regulation Of Oxysterol Formation, Steve Meaney Jan 2013

Epigenetic Regulation Of Oxysterol Formation, Steve Meaney

Articles

Oxysterols are oxygenated derivatives of cholesterol that may be formed by either enzymatic or non-enzymatic mechanisms. Expression of the genes responsible oxysterol synthesis (GROS) is known to be restricted across different tissues and cell types. Regulation of the transcription of GROS and the activity of their enzyme transcripts has been the subject of intense activity for many years. Recent studies have sought to decipher the mechanism(s) that underpin the restricted expression of the GROS. Available data indicates that epigenetic mechanisms have an important role to play in the control of the expression of GROS. In the current review we summarize …


Oxysterols And Parkinson´S Disease. Evidence That Levels Of 24s-Hydroxycholesterol In Cerebrospinal Fluid Correlates With The Duration Of The Disease., Steve Meaney, Anita Lovgren-Sandblom, Lovisa Brodin, Lisette Salveson, Valerio Leoni, Kristian Winge, Sven Palhagen, Per Svenninson Jan 2013

Oxysterols And Parkinson´S Disease. Evidence That Levels Of 24s-Hydroxycholesterol In Cerebrospinal Fluid Correlates With The Duration Of The Disease., Steve Meaney, Anita Lovgren-Sandblom, Lovisa Brodin, Lisette Salveson, Valerio Leoni, Kristian Winge, Sven Palhagen, Per Svenninson

Articles

Oxysterols are important for cholesterol homeostasis in the brain and may be affected in neurodegenerative diseases. The levels of the brain-derived oxysterol 24S-hydroxycholesterol (24S-OH) have been reported to be markedly reduced in the circulation of patients with Parkinson's disease (PD) (Lee et al., Antioxid. Redox Signal. 11 (2009) 407–420). The finding is surprising in view of the fact that other neurodegenerative diseases are associated with relatively modest effects on the circulating levels of 24S-OH. We determined the plasma and cerebrospinal fluid (CSF) levels of 24S-OH and 27-hydroxycholesterol (27-OH) in patients with PD with different disease duration using a highly accurate …


Socio-Economic Differences In Food Group And Nutrient Intakes Among Young Women In Ireland, Daniel Mccartney, Katherine Younger, John Kearney Jan 2013

Socio-Economic Differences In Food Group And Nutrient Intakes Among Young Women In Ireland, Daniel Mccartney, Katherine Younger, John Kearney

Articles

The present study aimed to investigate socio-economic disparities in food and nutrient intakes among young Irish women. A total of 221 disadvantaged and seventy-four non-disadvantaged women aged 18–35 years were recruited. Diet was assessed using a diet history protocol. Of the total population, 153 disadvantaged and sixty-three non-disadvantaged women were classified as plausible dietary reporters. Food group intakes, nutrient intakes and dietary vitamin and mineral concentrations per MJ of energy consumed were compared between the disadvantaged and non-disadvantaged populations, as was compliance with dietary fibre, macronutrient and micronutrient intake guidelines. The disadvantaged women had lower intakes than the non-disadvantaged women …


The Relationship Between Socioeconomic Status And Nutritional Knowledge In Women During Pregnancy, Michelle Lane, Ellen Barrett, Amy O'Higgins, Laura Mullaney, Michael Turner, Daniel Mccartney Jan 2013

The Relationship Between Socioeconomic Status And Nutritional Knowledge In Women During Pregnancy, Michelle Lane, Ellen Barrett, Amy O'Higgins, Laura Mullaney, Michael Turner, Daniel Mccartney

Articles

Epidemiological data have shown that socioeconomic status affects nutritional knowledge and dietary quality(1). Irish national food surveys have also suggested that socioeconomic differences in food group, nutrient intakes, dietary attitudes and health behaviours exist (2,3). It is known that pregnancy can enhance a women’s nutritional awareness (4). The aim of the present study was to assess whether nutritional knowledge in pregnant women in Ireland is affected by socioeconomic status. One hundred and sixteen pregnant women aged 16–41 years (mean age of 29.5 years) were recruited at their initial antenatal booking visit (10–15 weeks gestation) at a large Dublin maternity hospital. …


Obesity Is An Independent Predictor Of Breastfeeding Initiation When Socioeconomic Status Is Controlled For, Annemarie Bennett, Daniel Mccartney, John Kearney Jan 2013

Obesity Is An Independent Predictor Of Breastfeeding Initiation When Socioeconomic Status Is Controlled For, Annemarie Bennett, Daniel Mccartney, John Kearney

Articles

Breastfeeding initiation rates vary across Europe, with rates as low as 56%(1) in Ireland and rates in excess of 90%(2) in the Nordic countries. A host of factors, including demographic, social, psychological and physical factors, influence the initiation, intensity and duration of breastfeeding. The extent to which modifiable factors influence breastfeeding initiation should be ascertained in order to prioritise support services for breastfeeding. Obesity is a known modifiable factor which inhibits breastfeeding initiation. This study aimed to determine the extent to which obesity inhibits breastfeeding initiation while controlling for socioeconomic status and other confounding factors. The Infant Cohort dataset from …


Dietary Quality In A Sample Of Adults With Type 2 Diabetes Mellitus In Ireland; A Cross-Sectional Case Control Study, A Murray, A Mc Morrow, E O Connor, C Kiely, Oscar Mac Ananey, D O'Shea, M Egana, F Lithander Jan 2013

Dietary Quality In A Sample Of Adults With Type 2 Diabetes Mellitus In Ireland; A Cross-Sectional Case Control Study, A Murray, A Mc Morrow, E O Connor, C Kiely, Oscar Mac Ananey, D O'Shea, M Egana, F Lithander

Articles

BACKGROUND:

A number of dietary quality indices (DQIs) have been developed to assess the quality of dietary intake. Analysis of the intake of individual nutrients does not reflect the complexity of dietary behaviours and their association with health and disease. The aim of this study was to determine the dietary quality of individuals with type 2 diabetes mellitus (T2DM) using a variety of validated DQIs.

METHODS:

In this cross-sectional analysis of 111 Caucasian adults, 65 cases with T2DM were recruited from the Diabetes Day Care Services of St. Columcille's and St. Vincent's Hospitals, Dublin, Ireland. Forty-six controls did not have …


A Microfluidic Anti-Factor Xa Assay Device For Point-Of-Care Monitoring Of Anticoagulation Therapy, Leanne F. Harris, Paul Rainey, Vanessa Castro-Lopez, James S. O'Donnell, Anthony J. Killard Jan 2013

A Microfluidic Anti-Factor Xa Assay Device For Point-Of-Care Monitoring Of Anticoagulation Therapy, Leanne F. Harris, Paul Rainey, Vanessa Castro-Lopez, James S. O'Donnell, Anthony J. Killard

Articles

The development of new point-of-care coagulation assay devices is necessary due to the increasing number of patients requiring long-term anticoagulation in addition to the desire for appropriate, targeted anticoagulant therapy and a more rapid response to optimization of treatment. However, the majority of point-of-care devices currently available for hemostasis testing rely on clot-based endpoints which are variable and unreliable and are limited to measuring only certain portions of the coagulation pathway. There is a need for the introduction of point-of-care devices that can execute a broader range of tests; for example, the diagnosis of factor-specific diseases, as well as more …


Coagulation Monitoring Devices: Past, Present, And Future At The Point Of Care, Leanne F. Harris, Vanessa Castro-Lopez, Tony J. Killard Jan 2013

Coagulation Monitoring Devices: Past, Present, And Future At The Point Of Care, Leanne F. Harris, Vanessa Castro-Lopez, Tony J. Killard

Articles

Automated technologies have revolutionised the monitoring of coagulation disorders in the central hospital laboratory setting, allowing for high throughput testing, improved accuracy and precision, accompanied by a marked reduction in human error. However, they still require trained operators and sample transportation. With the advent of point of care (POC) testing, the working principle of traditional coagulometers was used as the foundation for the development of miniaturised devices. A number of POC coagulation devices have been commercially available for many years now, allowing the patient to assume more control over the management of their own medication, e.g. warfarin. While POC devices …


Effect Of Alveolar Bone Support On Zygomatic Implants – An Fea Study, Michael Ring, Michael Freedman, Leo F.A Stassen Jan 2013

Effect Of Alveolar Bone Support On Zygomatic Implants – An Fea Study, Michael Ring, Michael Freedman, Leo F.A Stassen

Articles

The objective of this study was to investigate the influence of maxillary alveolar bone on the stress distribution of zygomatic implants. A three-dimensional finite element model was created of half of a skull. Two zygomatic implants were modelled, placed in the skull supported by the zygomatic bone and the maxillary alveolar bone and connected by a fixed bridge. This model was duplicated, and the area of the maxillary alveolar bone supporting the implants was removed. Occlusal and lateral forces were applied to both models and the maximum von Mises stresses were recorded. Higher maximum stresses were noted in the model …


A Comparison Of Three Commercially Available Contrast-Detail Phantoms And Evaluation Of The Contrast-Detail Performance Under A Range Of Instrument Settings, Jacinta Browne, Des Hickey, Ashling Mcnabb Jan 2013

A Comparison Of Three Commercially Available Contrast-Detail Phantoms And Evaluation Of The Contrast-Detail Performance Under A Range Of Instrument Settings, Jacinta Browne, Des Hickey, Ashling Mcnabb

Articles

No abstract provided.


Effect Of Glycerol On A Diacetone Acrylamide-Based Holographic Photopolymer Material, Dervil Cody, Izabela Naydenova, Emilia Mihaylova Jan 2013

Effect Of Glycerol On A Diacetone Acrylamide-Based Holographic Photopolymer Material, Dervil Cody, Izabela Naydenova, Emilia Mihaylova

Articles

The composition of the low-toxicity, environmentally compatible diacetone acrylamide (DA) photopolymer has been modified with the inclusion of different additives. The addition of glycerol to the photopolymer composition is described. Results show that the incorporation of glycerol results in a uniform maximum refractive-index modulation for recording intensities in the range of 1–20  mW/cm2. This may be attributed to glycerol’s nature as a plasticizer, which allows for faster diffusion of an unreacted monomer within the grating during holographic recording. An optimum recording intensity of 0.5  mW/cm2 is observed for exposure energies of 20–60  mW/cm2. The modified photopolymer achieves a refractive-index modulation …


Standing Outside Of Article Iii, Tara Leigh Grove Jan 2013

Standing Outside Of Article Iii, Tara Leigh Grove

Articles

The Supreme Court has repeatedly insisted that standing doctrine is a "bedrock" requirement only of Article III Accordingly both jurists and scholars have assumed that the standing of the executive branch and the legislature like that of other parties depends solely on Article III But I argue that these commentators have overlooked a basic constitutional principle Federal institutions must have affirmative authority for their actions including the power to bring suit or appeal in federal court Article III defines the federal "judicial Power" and does not purport to confer any authority on the executive branch or the legislature Executive and …


Law's Violence: Derrick Bell's Next Article Challenging Authority: A Symposium Honoring Derrick Bell, Richard Delgado Jan 2013

Law's Violence: Derrick Bell's Next Article Challenging Authority: A Symposium Honoring Derrick Bell, Richard Delgado

Articles

No abstract provided.


Microsimulation Evaluation Of Eurocode Load Model For American Long-Span Bridges, Bernard Enright, Colm Carey, Colin Caprani Jan 2013

Microsimulation Evaluation Of Eurocode Load Model For American Long-Span Bridges, Bernard Enright, Colm Carey, Colin Caprani

Articles

The ability to accurately predict traffic loading is essential for cost-effective bridge maintenance and repair programs. The traffic load model currently used in the United States for the design of long-span bridges was developed over three decades ago. In the meantime vehicle characteristics and traffic patterns have changed. The Eurocode for traffic loading is more recent, but was calibrated only for bridges up to 200 m long. In this work, Weigh-In-Motion traffic records from 11 different sites across Alabama are used to establish congested traffic loading. Traffic microsimulation is used to generate the congestion, based on the real traffic data. …


A Short Chain Nad(H)-Dependent Alcohol Dehydrogenase (Hpscadh) From Helicobacter Pylori: A Role In Growth Under Neutral And Acidic Conditions., A. Kumari, Henry Windle, Denise Cornally, Barry Ryan, Gary Henehan Jan 2013

A Short Chain Nad(H)-Dependent Alcohol Dehydrogenase (Hpscadh) From Helicobacter Pylori: A Role In Growth Under Neutral And Acidic Conditions., A. Kumari, Henry Windle, Denise Cornally, Barry Ryan, Gary Henehan

Articles

Toxic aldehydes produced by alcohol dehydrogenases have been implicated in the pathogenesis of Helicobacter pylori-related damage to the gastric mucosa. Despite this, the enzymes that might be responsible for producing such aldehydes have not been fully described. It was, therefore, of considerable interest to characterize the alcohol oxidizing enzymes in this pathogen. Previous work in this laboratory characterized two such H. pylori enzymes that had broad specificity for a range of aromatic alcohol substrates. An enzyme with broad specificity for aliphatic alcohols is likely to be required in order that H. pylori can metabolize the wide range of substrates encountered …


Overview Of Approaches To Preventing And Avoiding Proteolysis During Expression And Purification Of Proteins, Barry Ryan, Gary Henehan Jan 2013

Overview Of Approaches To Preventing And Avoiding Proteolysis During Expression And Purification Of Proteins, Barry Ryan, Gary Henehan

Articles

Proteases are enzymes that cleave proteins. They occur widely in nature and serve a fundamental role in cellular homeostasis; however, their presence can result in unwanted protein degradation during recombinant protein expression and purification. This unit introduces proteases, specifically outlining the types commonly encountered during production of recombinant proteins. The strategies used to avoid and to prevent proteolysis are also highlighted with extensive consideration of the molecular, technical, and logistical methodologies involved