Digital Commons Network^™

Assessment Of Genetic Biodiversity Of Several Traits Using Ssr Markers In Rice (Oryza Sativa L.), Mahmoud Fazaa, Nensreen Nazmy Bassouony, Walaa Essa Essa, David A Lightfoot A. Lightfoot

Articles

Eight primers (RM 315, RM 318, RM 166, RM 302, RM 201, RM 234, RM 526 and RM 144) revealed different levels of polymorphism to tag the related traits of interest as tolerant to abiotic stress, resistant to biotic stress and yield-related traits. Two primers (RM 190 and RM 278) were monomorphic. The percentage of the polymorphism was nearly 80 %. The size of detected fragments ranged from 105–325 bp. A total of 186 bands were scored from the amplification products with the ten SSR primers. Genetic diversity analyses were conducted on the basis of the scores with 176 unique …

Magnetic Field Patterning Of Nickel Nanofibers Using Nickel Precursor Ink, Chaitanya G. Mahajan, Ahmed A. Alfadhel, Mark Irving, Bruce Kahn, David A. Borkholder, Scott A. Williams, Denis Cormier

Articles

This paper demonstrates an easily prepared novel material and approach to producing aligned nickel (Ni) nanowires having unique and customizable structures on a variety of substrates for electronic and magnetic applications. This is a new approach to producing printed metallic Ni structures from precursor materials, and it provides a novel technique for nanowire formation during reduction. This homogeneous solution can be printed in ambient conditions, and it forms aligned elemental Ni nanowires over large areas upon heating in the presence of a magnetic field. The use of templates or subsequent purification are not required. This technique is very flexible, and …

Cover Crops And Landscape Position Effects On Nitrogen Dynamics In Plant-Soil-Water Pools, Gurbir Singh, Karl Williard, Jon Schoonover, Kelly Nelson, Gurpreet Kaur

Articles

Nitrogen dynamics and water quality benefits deriving from the use of cover crops (CCs) are mostly incurred from plot-scale studies without incorporating large-scale variability that is induced by landscape positions. Our understanding of how topography affects the N response in CC systems is limited. The objectives of this study were to evaluate the effects of topography (shoulder, backslope, and footslope) and CCs (cereal rye, Secale cereale L. and hairy vetch, Vicia villosa L.) on nitrogen (N) uptake, soil inorganic N content (nitrate-N, NO3-N and total N, TN), and N leaching in watersheds that were planted with or without CCs. The …

Available Food Options At Local Shops In Relation To Food Insecurity Among Older Adults In Sharpeville, South Africa, Sanjoy Saha, Brenda Abu, Wilna Oldewage-Theron, Abdul Egal

Articles

Due to limited resources and complex food access systems in urban areas, especially older adults are more vulnerable to poor dietary intake and food insecurity in low-income areas in South Africa. This study assessed the prevalence of food insecurity among the older adults and explored the availability of healthy foods in local shops. It was a crosssectional study conducted in an eldercare centre in Sharpeville, South Africa with an estimated representative sample of 88 participants. Validated tools were used to measure the socio-economic, dietary diversity intake (24-hour dietary recall), household food security and access from the participants. Listing of available …

The Human Ortholog Of Archaeal Pus10 Produces Pseudouridine 54 In Select Trnas Where Its Recognition Sequence Contains A Modified Residue., Manisha Deogharia, Shaoni Mukhopadhyay, Archi Joardar, Ramesh Gupta

Articles

The nearly conserved U54 of tRNA is mostly converted to a version of ribothymidine (T) in Bacteria and eukaryotes and to a version of pseudouridine (Ψ) in Archaea. Conserved U55 is nearly always modified to Ψ55 in all organisms. Orthologs of TrmA and TruB that produce T54 and Ψ55, respectively, in Bacteria and eukaryotes are absent in Archaea. Pus10 produces both Ψ54 and Ψ55 in Archaea. Pus10 orthologs are found in nearly all sequenced archaeal and most eukaryal genomes, but not in yeast and bacteria. This coincides with the presence of Ψ54 in most archaeal tRNAs and some animal tRNAs, …

Cannabis For Medical Use: Fda And Dea Regulation In The Hall Of Mirrors, Rebecca S. Eisenberg, Deborah B. Leiderman

Articles

A majority of Americans now live in states that purport to authorize medical use of cannabis, although federal law continues to prohibit both recreational and medical use. The current legal regime for cannabis is unstable and may be more effective at deterring research than it is at deterring medical use. Lack of data on medical cannabis products poses public health risks as well as policy and legal challenges. Modified regulatory approaches for other kinds of products provide alternative models for encouraging safety and effectiveness research and providing better information about cannabis products already in clinical use.

Moving Towards The Second Generation Of Lignocellulosic Biorefineries In The Eu: Drivers, Challenges, And Opportunities, Shady S. Hassan, Gwilym A. Williams, Amit K. Jaiswal

Articles

The EU aims to achieve a variety of ambitious climate change mitigation and sustainable development goals by 2030. To deliver on this aim, the European Commission (EC) launched the bioeconomy strategy in 2012. At the heart of this policy is the concept of the sustainable Biorefinery, which is based centrally on a cost-effective conversion of lignocellulosic biomass into bioenergy and bioproducts. The first generation of biorefineries was based on utilization of edible food crops, which raised a “food vs. fuel” debate and questionable sustainability issues. To overcome this, lignocellulosic feedstock options currently being pursued range from non-food crops to agroforestry …

Biovr: A Platform For Virtual Reality Assisted Biological Data Integration And Visualization, Jimmy F. Zhang, Alex R. Paciorkowski, Paul A. Craig, Feng Cui

Articles

Background: Functional characterization of single nucleotide variants (SNVs) involves two steps, the first step is to convert DNA to protein and the second step is to visualize protein sequences with their structures. As massively parallel sequencing has emerged as a leading technology in genomics, resulting in a significant increase in data volume, direct visualization of SNVs together with associated protein sequences/structures in a new user interface (UI) would be a more effective way to assess their potential effects on protein function. Results: We have developed BioVR, an easy-to-use interactive, virtual reality (VR)-assisted platform for integrated visual analysis of DNA/RNA/protein sequences …

Comparison Of Heparg Cells Following Growth In Proliferative And Differentiated Culture Conditions Reveals Distinct Bioenergetic Profiles., Carolyn K J Young, Matthew J Young

Articles

HepaRG is a proliferative human hepatoma-derived cell line that can be differentiated into hepatocyte-like and biliary-like cells. Differentiated HepaRG cultures maintain key hepatic functions including drug transporters and xenobiotic-metabolizing enzymes. To gain insight into proliferative and differentiated HepaRG metabolism we profiled various bioenergetic parameters and investigated cell culture levels of adenosine triphosphate (ATP), lactate, and lactate dehydrogenase (LDH) activity. Compared to differentiated-derived HepaRG, cells from proliferative cultures had increased basal and ATP-linked respiration and decreased maximal and spare respiratory capacities. Basal ATP levels but not lactate or LDH activity were increased in samples from proliferative-derived compared to differentiated-derived HepaRG. Further …

Reckless Juveniles, Kimberly Thomas

Articles

Modern doctrine and scholarship largely take it for granted that offenders should be criminally punished for reckless acts.1 Yet, developments in our understanding of human behavior can shed light on how we define and attribute criminal liability, or at least force us to grapple with the categories that have existed for so long. This Article examines recklessness and related doctrines in light of the shifts in understanding of adolescent behavior and its biological roots, to see what insights we might attain, or what challenges these understandings pose to this foundational mens rea doctrine. Over the past decade, the U.S. Supreme …

Use Of Novel Anthropomorphic Breast Ultrasound Phantoms For Radiology Resident Education, Jacinta Browne, Chris Gu, Robert Fazzio, Andrew J. Fagan, Donald Tradup, Nicholas Hangiandreou

Articles

Purpose: This study evaluated the training and assessment role of anthropomorphic breast ultrasound phantoms which simulated both the morphological and sonographic characteristics of breast tissue, including lesions, in a group of radiology residents at a large academic medical center. Methods: This was a prospective study involving 9 residents across all years (2^nd–4^th year) of a radiology residency program. Baseline assessments of all residents ability to detect and characterize lesions in P-I were carried out, followed by a two-hour teaching session on the same phantom. All residents underwent a post-training, final assessment on P-II to evaluate changes in …

A Close-Up Look At Pcr, Callie J. Donahue, Ashley A. Adair, Leslie Kate Wright, Dina Newman

Articles

The Polymerase Chain Reaction (PCR) is a fundamental laboratory technique that allows for the amplification of many copies of a desired DNA target sequence. Despite its prevalence, undergraduate students often have poor comprehension about the underlying molecular mechanisms of PCR and the components necessary to carry out the reaction. We designed an interactive modeling activity to help students visualize the underlying molecular processes of denaturation, annealing, and extension, and to see how PCR parallels in vivo DNA replication. During the activity, students mimic denaturation, annealing, and extension by synthesizing DNA strands from individual nucleotides and primers in the 5’ to …

Combination Of Gold Nanoparticles With Low-Let Irradiation: An Approach To Enhance Dna Dsb Induction In Ht29 Colorectal Cancer Stem-Like Cells, Mahdi Abbasian, Zahra Arab-Bafrani, Azam Baharlouei, David Lightfoot

Articles

Purpose

High-linear energy transfer (high LET) irradiation has significant cytotoxic effects on different cancerous stem-like cells (CSLCs) such as colorectal CSLCs. A review of the literature has indicated that the presence of gold nanoparticles (GNPs) enables low-LET irradiation to produce highly non-homogeneous dose distributions like high-LET irradiation. The purpose of this study was to evaluate the radioresponsiveness of HT29 colorectal CSLCs under low-LET irradiation (X-ray) and in the presence of GNPs.

Methods

Radioresponsiveness was evaluated using the ϒ-H2AX foci formation assay, the clonogenic assay, the cell cycle progression assay and analyses of radiobiological parameters.

Results

In the presence of GNPs, …

Second Redemption, Third Reconstruction, Richard A. Primus

Articles

In The Accumulation of Advantages, the picture that Professor Owen Fiss paints about equality during and since the Second Reconstruction is largely a picture in black and white. That makes some sense. The black/white experience is probably the most important throughline in the story of equal protection. It was the central theme of both the First and Second Reconstructions. In keeping with that orientation, the picture of disadvantage described by Fiss’s theory of cumulative responsibility is largely drawn from the black/white experience. Important as it is, however, the black/white experience does not exhaust the subject of constitutional equality. So in …

Systems In Play: Simon Nicholson's Design 12 Course, University Of California, Berkeley, 1966, Tim Stott

Articles

In 1966, British artist, designer and educator Simon Nicholson (1934–1990) offered a lower division course, Design 12, at the College of Environmental Design, UC Berkeley. Controversially, Nicholson promoted play as the principal method of design and invited children to assess students’ projects on the Berkeley campus and in local schools, parks, playgrounds and hospitals. This article presents Design 12 as an important example of environmental design pedagogy in the USA, which uniquely attempted to synthesize British post-war constructivism with ‘design science’ and adventure play. The result was a course that placed play at the centre of design pedagogy, where it …

Nanomole Silver Detection In Chloride-Free Phosphate Buffer Using Platinum And Gold Micro- And Nanoelectrodes, Prabhakar Sidambaram, John Colleran

Articles

The electrochemical determination of ultra-low concentrations of silver requires reliable, reproducible measurements using sensitive analytical techniques. To date, the electrochemical determination of silver in biological buffers and pH neutral media has not been successful in terms of reproducibility. In this work, we report on the determination of ultra-low concentrations of silver in chloride-free phosphate buffer solution (PB, pH 7.4). Detection was conducted at gold and platinum micro and nanoelectrodes using anodic stripping voltammetry (ASV). The micro and nanoelectrodes were fabricated using a Sutter P-2000 laser puller, with physical and electrochemical characterization revealing flat disk-shaped working surfaces of 10–15 μm (microelectrode) …

'Elle T'Aime Trop, Et Moi, Pas Assez': Jacques Feyder's Melodramatic Mise En Scène Of Female Desire In Pension Mimosas (1935), Barry Nevin

Articles

Extract

Melodrama ‘à la française’: Feyder and French cinema of the 1930s

By the end of 1934, Jacques Feyder had led a distinguished career in French silent cinema, had directed a critically acclaimed adaptation of Émile Zola’s Thérèse Raquin (1928) in Berlin, had returned from a three-year contract in Hollywood, had brought Le Grand Jeu to the screen (the greatest box-office success of the 1933–34 season), and appeared to be virtually unstoppable as he proceeded to direct his next film, Pension Mimosas. The film was described by one critic as ‘sans aucun doute l’une des œuvres les plus attendues …

Activity Monitoring In Professional Soccer Goalkeepers During Training And Match Play, Victor Moreno-Pérez, Shane Malone, Lluís Sala-Pérez, Manuel Lapuente-Sagarra, Miguel Angel Campos-Vazquez, Juan Del Coso

Articles

The purpose of the present study was to quantify the external load of professional soccer goalkeepers. Twenty professional goalkeepers participated in the study. Data were classified according to the number of days before or after the match day (MD) as follows: MD-4, MD-3, MD-2, MD-1 for the sessions before the match, and MD+1 for the session after the match. The total running distance covered (TD), the high metabolic load (HMLD), the number of high metabolic load efforts (HMLE) were progressively reduced from MD-4 to MD-1 but the values of these variables were always inferior to MD (ES: -3.79 to −1.11). …

Fused Silica Capillary Interferometer With A Layer-By-Layer Functional Coating For The Analysis Of Chemicals Content In Aqueous Solutions, Arun Kumar Mallik, Gerald Farrell, Qiang Wu, Yuliya Semenova

Articles

A simple fused silica capillary interferometric (FSCI) sensor has been proposed and investigated for the detection and analysis of multiple chemical compounds content in aqueous solutions. The sensor was fabricated by splicing a commercially available fused silica capillary (FSC) with two single mode fibers to create a 0.7 cm long air cavity. The fiber surface was functionalized with two different polymers: poly (allylamine hydrochloride) (PAH) and sol-gel silica in sequence using a layer-by-layer deposition method. The operating principle of the sensor relies on light interference in the fused silica capillary cavity due to adhesion of the different chemical compounds on …

Ultra-High-Resolution Detection Of Pb2+ Ions Using A Black Phosphorus Functionalized Microfiber Coil Resonator, Yu Yin, Shunbin Wang, Shijie Jia, Jing Ren, Gerald Farrell, Elfed Lewis, Pengfei Wang

Articles

A black phosphorus (BP) functionalized optical fiber sensor based on a microfiber coil resonator (MCR) for Pb2+ ion detection in an aquatic environment is presented and experimentally demonstrated. The MCR-BP sensor is manufactured by winding a tapered microfiber on a hollow rod composed of a low-refractive-index polycarbonate (PC) resin with the BP deposited on the internal wall of the rod. Based on the propagation properties of the MCR, the chemical interaction between the Pb2+ ions and the BP alters the refractive index of the ambient environment and thus results in a detectable shift in the transmission spectrum. The resonance wavelength …

The Match-Play Sprint Performance Of Elite Senior Hurlers During Competitive Games, Damien Young, Giuseppe Coratella, Shane Malone, Kieran Collins, Laurent Mourot, Marco Beato

Articles

The typical sprint profile in elite hurling has yet to be established. The purpose of this study was to investigate the sprinting demands of elite hurling competition and characterize the sprinting patterns of different playing positions. GPS (10-Hz, STATSports Viper) were used to collect data from 51 hurlers during 18 games. The total sprint (≥22 km·h^-1) distance (TSD), the number of sprints (NOS) classified as length (<20 >m, ≥20 m) and relative speed thresholds (90%), the between-sprint duration and the number of repeated-sprint bouts (≥2 sprints in ≤60 s) were analyzed. The NOS was 22.2 ± 6.8 accumulating …

Anti-Hypochlorite And Catalytic Activity Of Commercially Available Moringa Oleifera Diet Supplement, Karolina Starzak, Bernadette Creaven, Arkadiusz Matwijczuk, Alicja Matwijczuk, Dariusz Karcz

Articles

Aiming at the assessment of the pro-health, and especially anti-hypochlorite properties of Moringa oleifera species a representative, commercially available Moringaoleifera dietary supplement was used as a substrate for the preparation of aqueous Moringa extract. The anti-hypochlorite activity of the extract was assessed using the hypochlorite-specific coumarin-based fluorescence turn-off sensor, namely 7-diethylamino-coumarin-3-carboxylic acid (7-DCCA). This compound was synthesized via the Knoevenagel condensation of 4-diethylamino-2-hydroxybenzaldehyde with Meldrum’sacidandtheMoringaextractwasemployedasamediumandcatalyst. Moreover,thetotal phenoliccontent(TPC)aswellasthereactiveoxygenspecies(ROS)–scavengingabilityoftheaqueous Moringa extract were determined. The results obtained demonstrated the applicability of Moringa extract as an anti-hypochlorite agent. Additionally, the satisfactory yield of the 7-DCCA obtained suggests the usefulness of the extract as a …

Dna Damage And Cytokine Production In Non-Target Irradiated Lymphocytes, Jane Bryant, Laura Shields, Christopher Hynes, Orla L. Howe, Brendan Mcclean, Fiona Lyng

Articles

In advanced radiotherapy, treatment of the tumor with high-intensity modulated fields is balanced with normal tissue sparing. However, the non-target dose delivered to surrounding healthy tissue within the irradiated volume is a potential cause for concern. Whether the effects observed are caused after exposure to out-of-field radiation or bystander effects through neighboring irradiated cells is not fully understood. The goal of this study was to determine the effect of exposure to out-of-field radiation in lymphocyte cell lines and primary blood cells. The role of cellular radiosensitivity in altering bystander responses in out-of-field exposed cells was also investigated. Target cells were …

Urban Aerobiological Risk Mapping Of Ornamental Trees Using A New Index Based On Lidar And Kriging: A Case Study Of Plane Trees, Raúl Pecero-Casimiro, Santiago Fernández-Rodríguez, Rafael Tormo-Molina, Alejandro Monroy-Colín, Inmaculada Silva-Palacios, Juan Pedro Cortés-Pérez, Ángela Gonzalo-Garijo, J. M. Maya-Manzano

Articles

Ornamental trees bring benefits for human health, including reducing urban pollution. However, some species, such as plane trees (Platanus sp.), produce allergenic pollen. Consequently, urban maps are a valuable tool for allergic patients and allergists, but they often fail to include variables that contribute to the “building downwash effect”, such as the width and shape of streets and the height of buildings. Other factors that directly influence pollen dispersion (slopes and other geographical features) also have not traditionally been discussed. The LiDAR (Laser Imaging Detection and Ranging) technique enables one to consider these variables with high accuracy. This work proposes …

Biosensor For The Oxidative Stress Biomarker Glutathione Based On Sam Of Cobalt Phthalocyanine On A Thioctic Acid Modified Gold Electrode, Mohammed Nooredeen Abbas, Ayman Ali Saeed, Mounir Ben Ali, Abdelhamid Errachid, Nadia Zine, Abdullatif Baraket, Baljit Singh

Articles

Self-assembled monolayer (SAM) of cobalt teraaminophthalocyanine (CoTAPc) was developed on thioctic acid (TA) dithiol modified gold electrode and electrochemically evaluated as a glutathione (GSH) selective biosensor. The CoTAPc-TA-Au modified electrode was developed by the covalent immobilization of the CoTAPc as the electrochemical mediator onto previously prepared gold electrode modified with TA (TA-Au) via amid bond formation with the carboxylic group of TA, producing well-organized SAM of the mediator. For comparison, another electrode modified with 3-mercaptopropionic acid (MPA) as a monothiol linker instead of TA was similarly prepared. The electrode surface modification was characterized using SEM, AFM, CV, and EIS. The …

Carbon Dioxide Measurement In Irish Blanket Peatlands: An Assessment Of Pool-Soil Flux Variability, Mariya Radomski, Alan Gilmer, Vivienne Byers, Eugene Mcgovern

Articles

Peatlands have been recognised as having a significant role in the mediation of atmospheric carbon dioxide (CO2) levels with direct implications for global climate change. Longitudinal in situ measurement systems for CO2 concentrations in blanket peatland ecosystems are difficult to implement where the nature of terrestrial–aquatic connectivity and hydrodynamics have a significant effect on the carbon cycle. The need for greater data on CO2 concentrations and flux monitoring in these settings has been well recognised. However, applying the most appropriate monitoring approach presents a special challenge. This paper sets out the development of a direct method for field based longitudinal …

Punishing Families For Being Poor: How Child Protection Interventions Threaten The Right To Parent While Impoverished, David Pimentel

Articles

No abstract provided.

Influence Of The Local Field And Dipole-Dipole Interactions On The Spectral Characteristics Of Simple Metals And Their Nanoparticles, I. I. Shaganov, Kevin Berwick, T. S. Perova

Articles

The effect of dipole-dipole interactions of free electrons on the spectral characteristics of simple metals and their nanoparticles is analyzed using Drude theory and the model of the Lorentz local field. It is established that accounting for the dispersion of a local field under conditions of one-dimensional (1D) confinement based on the optical constants of the bulk metal allows the determination of its spectral micro-characteristics in the frequency region of the longitudinal collective motions of the free electrons. This corresponds to the spectra of the dielectric losses of bulk plasma oscillations. A similar procedure for three-dimensional (3D) confinement produces the …

Talking About Black Lives Matter And #Metoo, Linda S. Greene, Lolita Buckner Innes, Bridget J. Crawford, Mehrsa Baradaran, Bennett Capers, Osamudia R. James, Keisha Lindsay

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No abstract provided.

Regulatory Bundling, Jennifer Nou, Jed Stiglitz

Articles

No abstract provided.