Digital Commons Network^™

In Vivo Mature Immunological Synapses Forming Smacs Mediate Clearance Of Virally Infected Astrocytes From The Brain, Carlos Barcia, Clare Thomas, James Curtin, Gwendalyn King, Kolja Wawrowsky, Marianela Candolfi, Weidong Xiong, Chunyan Liu, Kurt Kroeger, Olivier Boyer, Jerzy Kupiec-Weglinski, David Klatzmann, Maria Castro, Pedro Lowenstein

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The microanatomy of immune clearance of infected brain cells remains poorly understood. Immunological synapses are essential anatomical structures that channel information exchanges between T cell–antigen-presenting cells (APC) during the priming and effector phases of T cells' function, and during natural killer–target cell interactions. The hallmark of immunological synapses established by T cells is the formation of the supramolecular activation clusters (SMACs), in which adhesion molecules such as leukocyte function-associated antigen 1 segregate to the peripheral domain of the immunological synapse (p-SMAC), which surrounds the T cell receptor–rich or central SMAC (c-SMAC). The inability so far to detect SMAC formation in …

The Teaching-Research Relationship In Higher Education, Claire M. Mcdonnell

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This paper examines higher education policy regarding the relationship between research and teaching. The initial issue dealt with is whether current policy in Irish higher education recognises a relationship between research and teaching, and to examine the dual role of academics as teachers and as researchers. This is followed by an examination of any measures put in place to manage and balance the teaching–research relationship. The way in which the relationship between research and teaching is dealt with both in my own institution, the Technological University Dublin, and in higher education policy in a number of other countries is then …

Effect Of Ozone And Calcium Lactate Treaments On Browning And Textured Properties Of Fresh-Cut Lettuce, Daniel Rico, Ana Belen Martin-Diana, Gary Henehan, Jesus Maria Frias, Catherine Barry-Ryan

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The effects of three treatments, 1 mg L⁻¹ ozone at 18–20 °C, 15 g L⁻¹ calcium lactate (CLac) at 50 °C and a combination thereof, were compared on fresh-cut lettuce over 10 days of refrigerated storage. Respiration rate, browning and texture were examined as main quality indicators. The use of ozone produced a significantly (P < 0.05) higher oxygen decline than the use of CLac (from day 3 to day 10). At the end of storage, CLac (alone or combined with ozone) samples had higher oxygen content (∼9%) than ozone samples (∼6%). Enzymatic activity decreased significantly (P < 0.05) in ozone samples. Polyphenol oxidase activity in fresh-cut lettuce treated with ozone (alone or combined with CLac) showed lower values on day 1 (−1) and at the end of storage (g⁻¹) than CLac samples (4000–4800 units g⁻¹). Ozone also reduced peroxidase activity to ∼300 units g⁻¹ after treatment. Finally, pectin methylesterase activity was also reduced with ozone, showing a negative effect on textural properties. Data suggested that …

The Effect Of Physiological Cyclic Stretch On The Cell Morphology, Cell Orientation And Protein Expression Of Endothelial Cells, V. Barron, Claire Brougham, K. Coghlan, C. Stenson-Cox, D. O'Mahoney, P.E. Mchugh

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In vivo, endothelial cells are constantly exposed to pulsatile shear and tensile stresses. The main aim of this study was to design and build a physiological simulator, which reproduced homogenous strain profiles of the tensile strain experienced in vivo, and to investigate the effect of this cyclic tensile strain on the cell morphology, cell orientation and protein expression of endothelial cells. The biological response of human umbilical vein endothelial cells to a uniaxial cyclic stretch, in this newly developed simulator, was examined experimentally using immunohistostaining and confocal imaging and it was

found that the cells elongated and oriented at 58.9± …

Don't Go There: When To Abandon Lesson Plans And Venture, Humbly, Into Ground Zero, Sue Norton

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This paper discusses pedagogical styles that are appropriate in the classroom.

Color Interpretation: A Function Of Process & Film, Michael Peres

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Characteristics of modern color emulsions are reviewed and summarized by Professor Michael Peres particularly for Biomedical applications but expandable to other situations as well.

Applications Of Slow And Fast Streak Recording Cameras, Andrew Davidhazy

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This paper deals with two applications of streak photography in everyday industrial and biological research and development endeavors. In each instance, the simplicity and low cost with which the data could be reduced to useful information and the ease with which it could be communicated to other technical personnel was an important side benefit. In the first case, a laboratory built rotating drum streak camera with a 12" circumference, was used to study the performance of the new Olympus F-280 flash on an Olympus OM-4T camera. The strobing frequency, starting characteristics, duration under manual and camera control, flash relationship to …

Trial And Error: The Supreme Court's Philosophy Of Science, Susan Haack

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No abstract provided.

Lossless Compression Of Color Palette Images With One-Dimensional Techniques, Ziya Arnavut, Ferat Sahin

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Palette images are widely used on the World Wide Web (WWW) and in game-cartridge applications. Many images used on the WWW are stored and transmitted after they are compressed losslessly with the standard graphics interchange format (GIF), or portable network graphics (PNG). Well-known 2-D compression schemes, such as JPEG-LS and JPEG-2000, fail to yield better compression than GIF or PNG due to the fact that the pixel values represent indices that point to color values in a look-up table. To improve the compression performance of JPEG-LS and JPEG-2000 techniques, several researchers have proposed various reindexing algorithms. We investigate various compression …

Fms-Like Tyrosine Kinase 3 Ligand Recruits Plasmacytoid Dendritic Cells To The Brain, James Curtin, Gwendalyn King, Carlos Barcia, Chunyan Liu, Francois Hubert, Carole Guillonneau, Regis Josien, Ignacio Anegon, Pedro Lowenstein, Maria Castro

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The lack of professional afferent APCs in naive brain parenchyma contributes to the systemic immune ignorance to Ags localized exclusively within the brain. Dendritic cells (DCs) appear within the brain as a consequence of inflammation, but no molecular mechanisms accounting for this influx have been described. In this study we demonstrate that Fms-like tyrosine kinase 3 ligand (Flt3L) recruits plasmacytoid DCs (pDCs; >50-fold; p < 0.001) to the brain parenchyma. These pDCs expressed IFN-alpha, the hallmark cytokine produced by pDCs, indicating recruitment and activation in situ of bona fide pDCs within the brain parenchyma. Flt3L did not increase the numbers of conventional DCs, macrophages, or B, T, NK, NKT, or microglial cells within the brain. Our data demonstrate that Flt3L reconstitutes a crucial afferent component of the immune response, namely, professional APCs within the brain parenchyma, and this could counteract the intrinsic systemic immune ignorance to Ags localized exclusively within the brain.

Irish Intelligence Staff Work From Kosovo To Kabul, Tom Clonan

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Normally associated with routine troop deployments and logistic support to UN peace keeping and peace enforcement missions worldwide, the Irish Defence Forces have recently dramatically expanded their international intelligence presence abroad. In the wake of 9/11, Ireland’s Military Intelligence Directorate was expanded in order to assess emerging threats to the state – both external and internal – posed by global terrorist networks such as Al Qaeda. As an independent state agency, Ireland’s military intelligence are focused on long term trends within the global security environment across a broad spectrum of threats, from nuclear, biological and chemical weapons proliferation to the …

Truth Or Consequences: Settling Water Disputes In The Face Of Uncertainty, Barbara Cosens

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No abstract provided.

Attachment Theory And Wellbeing For The Young Person In Residential Care: The Provision Of A Second Chance Secure Base For The Child In Crisis, Gay Graham

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Aristotle argued that happiness for humans is not possible in the absence of reciprocal, affective relationships or friendships (Sherman 1991). Such relationships for children are only possible in the context of satisfactory attachments which provide for them a secure base from which to explore their environment (Bowlby 1988). Young people placed in the child welfare system, particularly those in residential care, often experience a system that is problem focused, intent on physical protection and control, where warm reciprocal relationships are not prioritised. This paper states that young people in residential care, whose primary attachments, whatever their quality, have been disrupted; …

Reactions Of 1,4-Bis(Tetrazole)Benzenes: Formation Of Long Chain Alkyl Halides, Fintan Kelleher, Andrew Bond, Adrienne Fleming, John Mcginley, Vipa Prajapati

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The reactions of 1,4-bis[2-(tributylstannyl)tetrazol-5-yl]benzene with a,w-dibromoalkanes were carried out in order to synthesise pendant alkyl halide derivatives of the parent bis-tetrazole. This led to the formation of several alkyl halide derivatives, substituted variously at N1 or N2 on the tetrazole ring. The crystal structures of 1,4-bis[(2-(4-bromobutyl)tetrazol-5-yl)]benzene (2-N, 2-N'), 1,4-bis[(2-(4-bromobutyl)tetrazol-5-yl)]benzene (1-N, 2-N') and 1,4-bis[(2-(8-bromooctyl)tetrazol-5-yl)]benzene (2-N, 2-N') are reported. Further discussion involves the structure of 1,4-bis(2-(6-bromohexyl)-2H-tetrazol-5-yl)benzene (2-N, 2-N') previously reported.

Apoptosis Is Initiated In Human Keratinocytes Exposed To Signalling Factors From Microbeam Irradiated Cells, Fiona Lyng, Paula Maguire, A. Kilmurray, Carmel Mothersill, C. Shao, M. Folkard, K. Prise

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Purpose: There is now no doubt that bystander signalling from irradiated cells occurs and causes a variety of responses in cells not targeted by the ionising track. However, the mechanisms underlying these processes are unknown and the relevance to radiotherapy and risk assessment remains controversial. Previous research by our laboratory has shown bystander effects in a human keratinocyte cell line, HPV-G cells, exposed to medium from irradiated HPV-G cells. The aim of this work was to investigate if similar mechanisms to those identified in medium transfer experiments occurred in these HPV-G cells when they are in the vicinity of microbeam …

Intuition, Custom, And Protocol: How To Make Sound Decisions With Limited Knowledge, Richard A. Epstein

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No abstract provided.

Efficient Responses To Catastrophic Risk, Richard A. Posner

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No abstract provided.

The Role Of The Judge In The Twenty-First Century, Richard A. Posner

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In my youthful, scornful way, I recognized four kinds of judgments; first the cogitative, of and by reflection and logomancy; second, aleatory, of and by the dice; third, intuitive, of and by feeling or "hunching;" and fourth, asinine, of and by an ass; and in that same youthful, scornful way I regarded the last three as only variants of each other, the results of processes all alien to good judges.

Childhood Sexual Abuse Among University Students In Tanzania, Denis Mccrann, Kevin Lalor, Joviter K. Katabaro

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Objectives: There are no prevalence data for childhood sexual abuse among Tanzanian university students. This investigation addressed this paucity. The nature of sexual abuse was also investigated. Method: Participants (N= 487) from a university in Tanzania completed a questionnaire which assessed abusive childhood sexual experiences, gathering information about age of victim, duration of abuse, perpetrators, amount of force or persuasion involved, and potential causes of child sexual abuse. A number of individuals were also interviewed about their experiences. Results: The overall prevalence rate for child sexual abuse was 27.7%, with rates being higher for females than for males. The average …

Colorblind Must Not Mean Blind To The Realities Facing Black Children, Zanita E. Fenton

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No abstract provided.

Climate Change And Animals Responses To Global Warming: The Law, Economics, And Science Of Climate Change, Cass R. Sunstein, Wayne Hsiung

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Climate change is already having adverse eeds on animal life, and those effects are likely to prove devastating in the future. Nonetheless, the relevant harms to animals have yet to become a serious part of the analysis of climate change policy. Even if animals and species are valued solely by reference to human preferences, consideration of animal welfare dramatically increases the argument for aggressive responses to climate change. We estimate that, even under conservative assumptions about valuation, losses to nonhuman life might run into the hundreds of billions of dollars annually. Whatever the precise figure, the general conclusion is clear: …

Government Secrets, Constitutional Law, And Platforms For Judicial Intervention, Adam M. Samaha

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American law has yet to reach a satisfying conclusion about public access to information on government operations. But recent events are prompting reconsideration of the status quo. As our current system is reassessed, three shortfalls in past debates should be overcome. The first involves ignorance of foreign systems. Other democracies grapple with information access problems, and their recent experiments are illuminating. Indeed they expose two additional domestic weaknesses. One is a line we have drawn within constitutional law. Courts and commentators tend to treat constitutional issues of public access separately from those of executive discretion to withhold information, but these …

The Absolute Prohibition Of Torture And Ill-Treatment, David Weissbrodt

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The President, the Secretary of State, and other U.S. government officials have repeatedly assured the world that the United States does not engage in "torture." Whenever they try to issue such statements, the critical listener must ask such questions as "What do they mean by torture?" Have they so narrowly defined "torture" as to ask the listener to overlook the mounting evidence of extremely brutal treatment which U.S. personnel have perpetrated against detainees in Afghanistan, Guantanamo, Iraq, and other secret detention facilities? Many detainees held by the U.S. have been subjected to illtreatment that would, under international definitions and jurisprudence, …

A Law And Economics Perspective On Terrorism, Francesco Parisi, Nuno Garoupa, Jonathan Klick

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This paper reviews the existing law and economics literature on crime, noting where various models might apply to the terror context. Specifically, it focuses on two strands of the literature, deterrence and incapacitation. It considers anti-terror measures enacted by different countries, highlighting how the details of the laws correspond to the insights from economic models of crime. In conclusion, the paper proposes an efficient sorting mechanism in which individuals will be provided with incentives to reveal their type to law enforcement authorities.

A Cognitive Theory Of Trust, Claire Hill, Erin O'Hara O'Connor

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Interpersonal trust is currently receiving widespread attention in the academy. Many legal scholars incorrectly assume that interpersonal trust is an unmitigated good (or bad) and that legal policy should therefore be crafted to maximize (or minimize) trust. A more nuanced understanding of trust indicates instead that it should be promoted or discouraged, depending on the context. Such an understanding needs to reflect the fact that trust and distrust can, and often do, coexist. In most relationships, the parties trust one another with regard to some matters and yet distrust one another with regard to other matters. More specifically, developing a …

The Involvement Of Calcium And Map Kinase Signaling Pathways In The Production Of Radiation-Induced Bystander Effects, Fiona Lyng, Paula Maguire, B. Mcclean, C. Seymour, Carmel Mothersill

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Much evidence now exists regarding radiation-induced bystander effects, but the mechanisms involved in the transduction of the signal are still unclear. The mitogen-activated protein kinase (MAPK) pathways have been linked to growth factor-mediated regulation of cellular events such as proliferation, senescence, differentiation and apoptosis. Activation of multiple MAPK pathways such as the ERK, JNK and p38 pathways have been shown to occur after exposure of cells to radiation and a variety of other toxic stresses. Previous studies have shown oxidative stress and calcium signaling to be important in radiation-induced bystander effects. The aim of the present study was to investigate …

Smart Growth: A Buffer Zone Between Decentrist And Centrist Theory?, Dorothy Stewart, Lorcan Sirr, Ruth Kelly

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The context for planning at the turn of the 19th century, in a newly industrialized world, was based on the need to find solutions to overcrowding and dire urban conditions. Planning decisions made in the post-World War II period were primarily motivated by the desire to reconstruct war torn cities. The forces of influence for planning and development in modern advanced capitalist societies are arguably set within the context of sustainable development. Many developed countries have witnessed a dramatic change in their territorial structures. Urban centres are extending into rural areas and surrounding hinterland, where large tracts of land are …

A Dose Threshold For A Medium Transfer Bystander Effect For A Human Skin Cell Line, Zhengfeng Liou, Carmel Mothersill, Fiona Mcneill, Fiona Lyng, Soo Hyun Byun, Colin Seymour, William Prestwich

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The existence of radiation-induced bystander effects mediated by diffusible factors is now accepted but the mechanisms and precise behavior at low doses remain unclear. We exposed cells to gamma doses in the range 0.04 mGy – 5 Gy, harvested the culture medium and transferred it to unirradiated reporter cells. Calcium fluxes and clonogenic survival were measured in the recipients. We show evidence for a dose threshold around 2 mGy for the human skin cell line used with a suggestion of increased survival below that dose. Similar experiments using direct gamma irradiation showed no reduction in survival until the dose exceeded …

Scientific Secrecy And "Spin": The Sad, Sleazy Saga Of The Trials Of Remune, Susan Haack

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No abstract provided.

Lessons On Lawyers, Democracy, And Professional Responsibility, Kenneth M. Rosen

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No abstract provided.