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Assessment Of An Osteoblast-Like Cell Line As A Model For Human Primary Osteoblasts Using Raman Spectroscopy, Lindsay Mcmanus, Franck Bonnier, George Burke, Brian Meenan, Adrian Boyd, Hugh Byrne

Articles

Raman spectroscopy is employed to determine the suitability of the U20S osteoblast-like cell line for use as a model for human primary osteoblasts, with emphasis on the ability of these cell types to replicate their tissue of origin. It was found that both cell types demonstrated early stage mineral deposition that followed significantly different growth patterns. Analysis of the growth pattern and spectral data from primary cells revealed increasing bone quality ratios and a high crystallinity, consistent with previous reports. Conversely the investigation of the U20S osteoblast-like cell line provided evidence of dense multilayered mineralised regions that corresponded more closely …

Genetic Analysis Of Relative Water Content (Rwc) In Two Recombinant Inbred Line Populations Of Soybean [Glycine Max (L.) Merr.], Salvador Virginia, Megan Pagan, Melissa Cooper, Stella K. Kantartzi, David A. Lightfoot, Khalid Meksem, My Abdelmajid Kassem

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Drought affects soybean [Glycine max (L.) Merr.] and other crops productivity in the US and other parts of the world. Relative water content (RWC) is an important indicator for plant water deficit tolerance (WDT). The objective of this study is to map quantitative trait loci (QTL) for RWC and several other leaf traits such as leaf dry weight (LDW), leaf fresh weight (LFW), and leaf turgid weight (LTW) in two soybean recombinant inbred line (RIL) populations, one derived from a cross of ‘Essex’ and ‘Forrest’ (ExF, n=94) and the other is derived from a cross of ‘PI 438489B’ and …

New Approaches To Selecting Resistance Or Tolerance To Sds And Fusarium Root Rot, Jiazheng Yuan, Rabia Bashir, Graciela Salas, Hemlata Sharma, Ali Srour, David A. Lightfoot

Articles

Fusarial rots are a significant problem worldwide affecting roots (and sometimes fruits) of most major crops including soybean, maize and wheat. Cultivar variation in partial resistance or tolerance is widespread and significant. Different cultivars of the soybean [Glycine max (L.) Merr.] have both resistance/tolerance to the leaf scorch known as Sudden Death Syndrome (SDS) and to the infection and root rot by the causal organism, Fusarium virguliforme (ex. F. solani f. sp glycines) hence the syndrome is composed of two diseases (1-3). Thirteen loci have been identified from analysis of 7 different crosses (2). Using new strains and new …

The Receptor Like Kinase At Rhg1-A/Rfs2 Caused Pleiotropic Resistance To Sudden Death Syndrome And Soybean Cyst Nematode As A Transgene By Altering Signaling Responses, Ali Srour, Ahmad Walid Afzali, Laureen Blahut-Beatty, Naghmeh Hemmati, Daina H. Simmonds, Wenbin Li, Miao Liu, Christopher D. Town, Hemlata Sharma, Prakash Arelli, David A. Lightfoot

Articles

Background: Soybean (Glycine max (L. Merr.)) resistance to any population of Heterodera glycines (I.), or Fusarium virguliforme (Akoi, O’Donnell, Homma & Lattanzi) required a functional allele at Rhg1/Rfs2. H. glycines, the soybean cyst nematode (SCN) was an ancient, endemic, pest of soybean whereas F. virguliforme causal agent of sudden death syndrome (SDS), was a recent, regional, pest. This study examined the role of a receptor like kinase (RLK) GmRLK18-1 (gene model Glyma_18_02680 at 1,071 kbp on chromosome 18 of the genome sequence) within the Rhg1/Rfs2 locus in causing resistance to SCN and SDS.

Results: A BAC (B73p06) …

Is A Self-Catering Holiday With The Family Really A Holiday For Mothers? Examining The Balance Of Household Responsibilities While On Holiday From A Female Perspective, Ziene Mottiar, Deirdre Quinn

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The commonly cited definition of what constitutes a holiday is that it is a change from the norm, or an escape from everyday life. But is this the case if tourists are going on a self-catering holiday where many of the tasks from everyday life such as cleaning, minding children and cooking must still be undertaken? This research is specifically interested in the role of mothers, from their own perspectives, on such holidays. It explores how household responsibilities are divided between partners when on holiday and questions does this differ from the situation when at home? In so doing this …

Using A Minimum Tile Path For Plant Transformations Encompassing The Entire Soybean Genome, Hidayat Ullah, Mohammed Jasim, David A. Lightfoot

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Genomes like Glycine max (soybean) that have been highly conserved following increases in ploidy represent a frontier for genome analysis. Many soybean QTL analyzed to date have been composed of gene clusters each with contributing a portion of the trait rather than alleles of single genes. At the Soybean Genome Database (SoyGD) http://soybeangenome.siu.edu the genome browser that integrates and served the publicly available soybean physical map, BAC fingerprint database and genetic map associated genomic data shows a minimum tile of transformation ready BIBAC-like clones in pCLD04541 (pV41; oriV; tra; bom). Sequence resources made available through the DOE genome sequencing project …

Photonic Crystal Fiber Interferometer For Dew Detection, Jinesh Mathew, Yuliya Semenova, Gerald Farrell

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A novel method for dew detection based on photonic crystal fiber (PCF) interferometer that operates in reflection mode is presented in this paper. The fabrication of the sensor head is simple since it only involves cleaving and fusion splicing. The sensor shows good sensitivity to dew formation with a large wavelength peak shift of the interference pattern at the onset of dew formation. The device’s response to ambient humidity and temperature are also studied and reported in this paper. From our experiment it is also concluded that by attaching a thermoelectric cooler with temperature feedback, the sensor head demonstrated can …

Bacterial Host Interactions In Cystic Fibrosis, Máire Callaghan, Siobhan Mcclean

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Chronic infection is a hallmark of cystic fibrosis (CF) and the main contributor to morbidity. Microbial infection in CF is complex, due to the number of different species that colonise the CF lung. Their colonisation is facilitated by a host response that is impaired or compromised by highly viscous mucous, zones of hypoxia and the lack of the cystic fibrosis transmembrane regulator (CFTR). Successful dominant CF pathogens combine an effective arsenal to establish infection and counter-attack the host response, together with an ability to adapt readily to an unfavourable environment. Hypermutability is common among CF pathogens facilitating adaptation and as …

Using Conservation Management Agreements To Secure Postrecovery Perpetuation Of Conservation-Reliant Species: The Kirtland's Warbler As A Case Study, Dale Goble

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Kirtland’s warbler is one of many conservation-reliant species listed under the Endangered Species Act (ESA). This species has met recovery goals, but removing it from the protections of the ESA is problematic because of its reliance on ongoing conservation. We define conservation management agreements (CMAs) and describe how they may provide a mechanism to protect conservation-reliant species after delisting. We suggest that CMAs should include four major elements: (1) a conservation partnership capable of implementing management actions at conservation-relevant scales, (2) a conservation management plan based on the management actions in the species’ successful recovery plan, (3) sufficient financial resources …

Coordination Studies Of Copper(Ii), Cobalt(Ii) And Iron(Ii) With Isomeric Pyridyl-Tetrazole Ligands, Fintan Kelleher, Andrew Bond, Adrienne Fleming, Jackie Gaire, John Mcginley, Vickie Mckee, Ursula Sheridan

Articles

The reaction of 2-(2H-tetrazol-5-yl)pyridine (L1) with 1,6-dibromohexane results in formation of the isomers 2-(6′′-bromohexyl-(1-tetrazol-5-yl)pyridine (L2) and 2-(6′′-bromohexyl-(2-tetrazol-5-yl)pyridine (L3). Coordination reactions of L2 and L3 with CuCl₂·2H₂O, Co(SCN)₂ and Fe(ClO₄)₂·H₂O yielded the strongly coloured solids [Cu(II)(L2)Cl₂]₂ (1), [Cu(II)(L3)Cl₂]₂ (2), [Co(II)(L2)₂(NCS)₂] (3), [Co(II)(L3)₂(NCS)₂] (4), [Fe(II)(L2)₂(H₂O)₂](ClO₄) …

Too Rough A Justice: The Ethiopia-Eritrea Claims Commission And Civil Liability For Claims For Rape Under International Law, Ryan S. Lincoln

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The developments in international law prohibiting rape during armed conflict have grown at a rapid pace in recent decades. Whereas rape had long been considered an inevitable by-product of armed conflict, evolution in international humanitarian law (IHL) has relegated this conception mostly to the past. The work of international criminal tribunals has been at the forefront of this change, developing the specific elements of the international crime of rape, and helping to change the perception of rape in international law violations of IHL, however also give rise to civil liability Despite the advances with respect to rape made in the …

Review Of The Rpt3 Genes Encoding Part Of The 26s Proteasome Associated With Loci Underlying Disease Resistance In Soybean, Shivani Malik, Sukesh Bhaumik, David A. Lightfoot

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The 26S proteasomal complex is a multifunctional proteolytic machinery of the cell. The proteasome plays role in myriad of cellular functions, which have been further diversified by its separable proteolytic and non-proteolytic sub-complexes. Protein quality control and turnover, cell cycle regulation, gene regulation and DNA repair are among the key processes controlled by the proteasome. Disease resistance in plants invokes changes in all the processes controlled by the 26S proteasome. In this review, the potential contribution of genes encoding the proteasome to disease resistance in soybean (Glycine max L. Merr.) was examined.

Increased Susceptibility Of Arterial Tissue To Wire Perforation With The Application Of High Frequency Mechanical Vibrations, Mark Wylie, Garrett Mcguinness, Graham Gavin

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High frequency mechanical vibrations (20–50 kHz), delivered via small diameter flexible wire waveguides represent a minimally invasive technology for the treatment of chronic total occlusions (CTOs) and in other tissue ablation applications. Tissue disruption is reported to be caused by repetitive mechanical contact and cavitation. This work focuses on the effects of vibrating wire waveguides in contact with arterial tissue. An apparatus with clinically relevant parameters was used, characterized as operating at 22.5 kHz and delivering amplitudes of vibration of 17.8 - 34.3 µm (acoustic intensity, I_SATA: 1.03 - 3.83 W/cm²) via 1.0 mm diameter waveguides. …

A State-Based National Network For Effective Wildlife Conservation, Dale Goble

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State wildlife conservation programs provide a strong foundation for biodiversity conservation in the United States, building on state wildlife action plans. However, states may miss the species that are at the most risk at rangewide scales, and threats such as novel diseases and climate change increasingly act at regional and national levels. Regional collaborations among states and their partners have had impressive successes, and several federal programs now incorporate state priorities. However, regional collaborations are uneven across the country, and no national counterpart exists to support efforts at that scale. A national conservation-support program could fill this gap and could …

Resilience And Water Governance: Adaptive Governance In The Columbia River Basin, Barbara Cosens

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The 1964 Columbia River Treaty between the United States and Canada is currently under review. Under the treaty, the river is jointly operated by the two countries for hydropower and is the largest producer of hydropower in the western hemisphere. In considering the next phase of international river governance, the degree of uncertainty surrounding the drivers of change complicates efforts to predict and manage under traditional approaches that rely on historical ecosystem responses. At the same time, changes in social values have focused attention on ecosystem health, the decline of which has led to the listing of seven salmon and …

The Natural Law Bridge Between Private Law And Public International Law, Richard A. Epstein

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One keg similarity between natural law and international law is that they seek to obtain a stable social order without the intervention of any state authority capable of issuing and enforcing its commands to those subject to its rule. Among ordinary individuals in a state of nature that weakness tends to lead to a stripped-down libertarian regime that features simple rules of acquisition, contract, and protection. The system gains its relative stability from the brute fact that between rough equals the part in the defensive posture will win out, so that great disparities in power are needed to disrupt the …

Citizens United And Conservative Judicial Activism, Geoffrey R. Stone

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This Article analyzes the recent trend of conservative judicial activism in the Supreme Court and searches for a principled reason to explain it. The conservative majority has struck down several laws in recent years, culminating in its invalidation of an important provision of the Bipartisan Campaign Reform Act of 2002 in Citizens United v. Federal Election Commission. While judicial restraint and originalism are currently seen as conservative principles, neither principle explains these decisions. The author argues that no principle can explain the results of these cases-rather, they can only be explained by the Justices' personal views and policy preferences. The …

Maritime Health: A Review With Suggestions For Research, Malcolm Maclachlan, Bill Kavanagh, Alison Kay

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International maritime health has largely developed within the sphere of occupational health services and international health problems. We reviewed publications in the journal International Maritime Health from 2000 to 2010 to establish the coverage of the journal and the scope of research in maritime health. We identified six thematic categories: healthcare access, delivery and integration; telehealth; non-communicable diseases and physical health problems; communicable diseases; psychological functioning and health; and safety-related issues. We describe the research within these themes and report on their publication prominence. We also analyse the research in terms of its geographical focus, the population groups addressed and …

The Gap In Law Between Developmental Expectations And Educational Obligations, Emily Buss

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No abstract provided.

The Doctrinal Paradox & International Law, Adam S. Chilton, Dustin Tingley

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No abstract provided.

Aggregation And Law, Ariel Porat, Eric A. Posner

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If a plaintiff brings two claims, each with a 0.4 probability of being valid, the plaintiff will usually lose, even if the claims are based on independent events, and thus the probability of at least one of the claims being valid is 0.64. If a plaintiff brings two independent claims, and neither of them alleges misconduct sufficient to justify a remedy, the plaintiff will usually lose, even if the claims jointly allege sufficient wrongdoing to justify a remedy. Thus, as a general rule, courts refuse to engage in what we call factual aggregation (the first case) and normative aggregation (the …

Absolute Preferences And Relative Preferences In Property Law, Lior Strahilevitz

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No abstract provided.

Resilience And Law As A Theoretical Backdrop For Natural Resource Management: Flood Management In The Columbia River Basin, Barbara Cosens

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The 1964 Columbia River Treaty entered by the United States and Canada for mutual benefits in flood control and hydropower generation is under review in anticipation of expiration of certain flood control provisions in 2024. This Article asserts that nonstructural measures should be the primary focus of new expenditure on flood risk management in the Columbia River Basin over the next sixty-year period of treaty implementation to align flood risk management with management for ecosystem resilience. Resilience is the measure of the capacity of a system to maintain important functions, structures, identity, and feedback through adaptation in the face of …

Overcoming Neoliberal Hegemony In Community Development: Law, Planning, And Selected Lamarckism, Jerrold A. Long

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No abstract provided.

Conservation Reliant-Species, Dale Goble

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A species is conservation reliant when the threats that it faces cannot be eliminated, but only managed. There are two forms of conservation reliance: population- and threat-management reliance. We provide an overview of the concept and introduce a series of articles that examine it in the context of a range of taxa, threats, and habitats. If sufficient assurances can be provided that successful population and threat management will continue, conservation-reliant species may be either delisted or kept off the endangered species list. This may be advantageous because unlisted species provide more opportunities for a broader spectrum of federal, state, tribal, …

An Essay On Slavery's Hidden Legacy: Social Hysteria And Structural Condonation Of Incest, Zanita E. Fenton

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No abstract provided.

Cognitive Warfare And Young Black Males In America, Perry L. Moriearty, William Carson

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The result was a veritable feedback loop whose cognitive output, the mental imprint of "morally impoverished" "super- predators,"14 continually fed its input. [...]even as crime rates among black youth have dropped steadily since the mid-1990s, these selfreinforcing associations and dissociations have prompted lawmakers and their constituents to continue to support laws and policies that they know disproportionately punish and incapacitate young black males. [...]is the apparent extent to which even subliminal racial primes can influence our perceptions of individuals.

Litigation's Role In The Path Of U.S. Federal Climate Change Regulation: Implications Of Aep V. Connecticut, Hari M. Osofsky

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This symposium analyzes the role of litigation in climate change regulation, with a particular focus on the U.S. Supreme Court's June 2011 decision in American Electric Power Co. v. Connecticut ("AEP"). 1 This Essay adds to that conversation by exploring the significance of AEP for U.S. federal legal approaches to regulating climate change.

Deidentification And Reidentification In Returning Individual Findings From Biobank And Secondary Research: Regulatory Challenges And Models For Management, William Mcgeveran, Leili Fatehi, Pari Mcgarraugh

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No abstract provided.

Siblings In Law, Jill Elaine Hasday

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No abstract provided.