Digital Commons Network^™

Thermostability Of The Coating, Antigen And Immunostimulator In An Adjuvanted Oral Capsule Vaccine Formulation, Stephanie Longet, Vincenzo Aversa, Daire O'Donnell, Joshua Tobias, Monica Rosa, Jan Holmgren, Ivan Coulter, Ed Lavelle

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Oral vaccines present an attractive alternative to injectable vaccines for enteric diseases due to ease of delivery and the induction of intestinal immunity at the site of infection. However, susceptibility to gastrointestinal proteolysis, limited transepithelial uptake and a lack of clinically acceptable adjuvants present significant challenges. A further challenge to mass vaccination in developing countries is the very expensive requirement to maintain the cold chain. We recently described the effectiveness of a Single Multiple Pill® (SmPill®) adjuvanted capsule approach to enhance the effectiveness of a candidate enterotoxigenic Escherichia coli (ETEC) oral vaccine. Here it was demonstrated that this delivery system …

Mind The Gap: An Initial Analysis Of The Transition Of A Second Level Curriculum Reform To Higher Education, Michael Carr, Fiona Faulkner, Cormac Breen, Mark Prendergast

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This article details an initial analysis of the transition of a second level curriculum reform to higher education in Ireland. The reform entitled ‘Project Maths’ involved changes to what second level students learn in mathematics, how they learn it, and how they are assessed. Changes were rolled out nationally on a phased basis in September 2010. Students who were taught and assessed through the new curriculum first entered third level education in September 2012. It is important that third level mathematics lecturers are aware of the changes to the curriculum since certain topics such as vectors and matrices are no …

Studying The Drug Delivery Kinetics Of A Nanoporous Matrix Using A Mip-Based Thermal Sensing Platform, Christopher J. Pawley, Ariane Perez-Gavilan, Kaelin S. Foley, Sarah Lentink

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The implementation of Molecularly Imprinted Polymers (MIPs) into sensing systems has been demonstrated abundantly over the past few decades. In this article, a novel application for an MIP-based thermal sensing platform is introduced by using the sensor to characterize the drug release kinetics of a nanoporous silver-organic framework. This Ag nanoporous matrix was loaded with acetylsalicylic acid (aspirin) which was used as a model drug compound in this study. The drug elution properties were studied by placing the nanoporous matrix in phosphate buffered saline solution for two days and measuring the drug concentration at regular time intervals. To this extent, …

A National Audit Of Smoking Cessation Services In Irish Maternity Units, C. Reynolds, B. Egan, Shona Cawley, S. Sheehan, M. Turner

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Irish Medical Journal

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Pcr-Ribotype Distribution Of Clostridium Difficile In Irish Pigs, Katharina Stein, Sarah Egan, Helen Lynch, Celine Harmanus, Lorraine Kyne, Celine Herra, Sinead Mcdermott, Ed Kuijper, Fidelma Fitzpatrick, Susan Fitzgerald, Lynda Fenelon, Denise Drudy

Articles

Clostridium difficile is an important enteric pathogen in humans causing infections in the healthcare environment and the community. Carriage of C. difficile and C. difficile-related enterocolitis has been reported in piglets worldwide. The aim of this study was to investigate the rates of C. difficile isolation from pigs in Ireland. Faecal samples from piglet litters and sows were collected from six farms in 2015. The sows were non-diarrhoeal at the time of sampling. The diarrhoeal status of the piglets was unknown. C. difficile was isolated from 34/44 (77%) of piglet litter samples and from 33/156 (21%) of sow samples. The …

There's No Place Like Home: Reshaping Community Interventions And Policies To Eliminate Environmental Hazards And Improve Population Health For Low-Income And Minority Communities, Emily A. Benfer, Allyson E. Gold

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Substandard housing and environmental conditions threaten the health and wellbeing of individuals residing throughout the United States Empirical evidence on the relationship between housing and health has increased exponentially However despite the growth in research residents continue to be exposed to environmental health hazards Minorities and people in poverty are exposed to environmental health hazards at a disproportionately high rate Hazards such as lead mold pest infestation radon and carbon monoxide among others threaten individual safety and health and limit one's ability to access opportunity in society Moreover the effects of exposure can be farreaching Common approaches to healthy communities …

Bankrupt Marketplace: First Amendment Theory And The 2016 Presidential Election, Leonard M. Niehoff

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In this article I advance two arguments. The first is that 2016 was a particularly important year for freedom of speech and the press, although not for conventional reasons. The second is that hte events of 2016 revealed that one of the essential components of our democracy - the central role that free expression plays in the democratic process - is in a state of serious dysfunction, if not crisis.

Are Trump's Attacks On The Media Adversely Affecting Public Opinion?, Leonard M. Niehoff

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Both during the election cycle and as president of the United States, Donald Trump has enthusiastically and aggressively attacked the media. On Twitter, in speeches, and at rallies he has repeatedly deployed his favorite “f words” against mainstream broadcast, print, and online news sources: “fake,” “fraudulent,” “failing,” and (phonetically) “phony.” Some attacks have been personal to individual journalists, some have been more institutionally focused, and some have been made in contexts that appeared to create physical risk to reporters who were present. But whatever the variation in lavors, the frequency of the attacks has remained constant. Indeed, Trump has devoted …

Contemporary Practice Of The United States Relating To International Law, January 2017, Daugirdas Kristina, Julian Davis Mortenson

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In this section: • Congress Overrides Obama’s Veto to Pass Justice Against Sponsors of Terrorism Act • U.S. Federal Court of Appeals Upholds United Nations’ Immunity in Case Related to Cholera in Haiti • U.S.-Russian Agreements on Syria Break Down as the Syrian Conflict Continues • Russia Suspends Bilateral Agreement with United States on Disposal of Weapons-Grade Plutonium • The United States Makes Payment to Family of Italian Killed in CIA Air Strike • United States Ratifies Hague Convention on the International Recovery of Child Support and Other Forms of Family Maintenance

The Sec's Shift To Administrative Proceedings: An Empirical Assessment, Stephen J. Choi, Adam C. Prichard

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Congress has repeatedly expanded the authority of the SEC to pursue violations of securities laws in proceedings adjudicated by the SEC's own administrative law judges, most recently through the Dodd-Frank Act. We report the results from an empirical study of SEC enforcement actions against non-financial public companies to assess the impact of the Dodd-Frank Act on the balance between civil court and administrative enforcement actions. We show a general decline in the number of court actions and an increase in the number of administrative proceedings post-Dodd-Frank. At the same time, we show an increase in average civil penalties post-Dodd-Frank for …

What We Think, What We Know And What We Think We Know About False Convictions, Samuel Gross

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False convictions are notoriously difficult to study because they can neither be observed when they occur nor identified after the fact by any plausible research strategy. Our best shot is to collect data on those that come to light in legal proceedings that result in the exoneration of the convicted defendants. In May 2012, the National Registry of Exonerations released its first report, covering 873 exonerations from January 1989 through February 2012. By October 15, 2016, we had added 1,027 cases: 599 exonerations since March 1, 2012, and 428 that had already happened when we issued our initial report but …

Design-Based Research Mobile Gaming For Learning Jewish History, Tikkun Olam, And Civics, Owen Gottlieb

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How can Design-Based Research (DBR) be used in the study of video games, religious literacy, and learning? DBR uses a variety of pragmatically selected mixed methods approaches to design learning interventions. Researchers, working with educators and learners, design and co-design learning artifacts and environments. They analyze those artifacts and environments as they are used by educators and learners, and then iterate based on mixed methods data analysis. DBR is suited for any "rich contextualized setting in which people have agency." (Hoadley 2013) such as formal or informal learning environments.

The case covered in this chapter is a mobile Augmented Reality …

How Much Wind And Solar Are Needed To Realize Emissions Benefits From Storage?, Srujana Naga Goteti, Eric Hittinger, Eric Williams

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Environmental outcomes from energy storage depend on its usage patterns, the existing generation fleet, and fossil fuel prices. This work models the deployment of large, non-marginal quantities of energy storage and wind and solar power to determine their combined effects on grid system emissions. Two different grid environments are analyzed: a coal-heavy grid (Midcontinent ISO) and non-coal grid (New York ISO). An iterative dispatch model is used that operates storage to maximize income, considering that this operation can influence wholesale energy prices. With current low natural gas prices ($2.6 per MMBtu), adding storage slightly reduces carbon emissions in New York, …

To Be Or Not To Be T4: Evidence Of A Complex Evolutionary Pathway Of Head Structure And Assembly In Giant Salmonella Virus Spn3us, Bazla Ali, Maxim Desmond, Sara Mallory, Andrea Benitez, Larry J. Buckley, Susan T. Weintraub, Michael V. Osier, Lindsay W. Black, Julie A. Thomas

Articles

Giant Salmonella phage SPN3US has a 240-kb dsDNA genome and a large complex virion composed of many proteins for which the functions of most are undefined. We recently determined that SPN3US shares a core set of genes with related giant phages and sequenced and characterized 18 amber mutants to facilitate its use as a genetic model system. Notably, SPN3US and related giant phages contain a bolus of ejection proteins within their heads, including a multi-subunit virion RNA polymerase (vRNAP), that enter the host cell with the DNA during infection. In this study, we characterized the SPN3US virion using mass spectrometry …

Career Development And Personal Success Profile Of Students - Followers And Students - Potential Future Leaders: The Case Of Rit Croatia, Jasminka Samardzija, Joseph Kevin Walker, Vanda Bazdan

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This study examines the differences among students in terms of self-reported leadership characteristics. It was conducted among all three programs and four generations of undergraduates at RIT Croatia. The goal of this study is to determine the differences among students who report being more and less leadership prone (Potential Future Leaders and Followers, respectively) with regard to demographic characteristics, reported self reliance socialization pattern, college level and program choice, career focus attainment and development, and reported attitudes regarding the importance of specific personality traits in leadership, the importance of specific career development factors and success indicators. Research showed that generation …

Work Placement Reflective Assessments And Employability Enhanced Through Highlighting Graduate Attributes, Julie Dunne

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This paper reports on a study which investigated the effect of activities to promote awareness of specific prioritised graduate attributes on the quality of reflection displayed in student work-placement reflective blog assessments. The focus of the paper is on the results from a thematic analysis of reflective writing assisted by NVivo software from a control and research group, using the a priori codes of ‘reflection’ and ‘graduate attributes’, as part of a Participatory Action Research study. The findings show an increase in reflection associated with graduate attributes in the research group compared to the control group. More importantly, there is …

Controlling Brochothrix Thermosphacta As A Spoilage Risk Using In Package Atmospheric Cold Plasma, Apurva Patange, Daniela Boehm, Carmen Bueno-Ferrer, Patrick J. Cullen, Paula Bourke

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Brochothrix thermosphacta is a predominant spoilage microorganism in meat and its control in processing environments is important to maintain meat product quality. Atmospheric cold plasma is of interest for control of pathogenic and spoilage microorganisms in foods. This study ascertained the potential of dielectric barrier discharge atmospheric cold plasma (DBD-ACP) for control of B. thermosphacta in response to key parameters such as treatment time, voltage level, interactions with media composition and post treatment storage conditions. Challenge populations were evaluated as suspensions in PBS, as biofilms in meat model medium and surface attached on raw lamb chops under MAP.

ACP treatment …

Risk Registers: Structuring Data Collection To Develop Risk Intelligence, Maria Chiara Leva, Nora Balfe, B. Mcaleer, M. Rock

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This paper presents the results of the development and implementation of a company-wide risk register, based on a clear set of data structures. A case study from an electricity generation company is presented and the process followed is described. The results of the case study indicated areas where the concept of risk registers could be extended to make better use of existing data and to support continuous improvement of risk management. Six key areas are discussed (1) aggregation of risks across the business, (2) supporting controls over mitigation measures, (3) improved estimation of event likelihood, (4) integrating with critical asset …

Microbiological Interactions With Cold Plasma, Paula Bourke, Dana Ziuzina, Lu Han, Patrick J. Cullen, Brendan Gilmore

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ere is a diverse range of microbiological challenges facing the food, healthcare and clinical sectors. The increasing and pervasive resistance to broad-spectrum antibiotics and health-related concerns with many biocidal agents drives research for novel and complementary antimicrobial approaches. Biofilms display increased mechanical and antimicrobial stability and are the subject of extensive research. Cold plasmas (CP) have rapidly evolved as a technology for microbial decontamination, wound healing and cancer treatment, owing to the chemical and bio-active radicals generated known collectively as reactive oxygen and nitrogen species. This review outlines the basics of CP technology and discusses the interactions with a range …

Highly Branched Poly(5-Amino-1-Pentanol-Co-1,4- Butanediol Diacrylate) For High Performance Gene Transfection, Ming Zeng, Dezhong Zhou, Singwei Ng, Jonathan O'Keefe Ahern, Fatma Alshehri, Yongsheng Gao, Luca Pierucci, Udo Greiser, Wenxin Wang

Articles

The top-performing linear poly(β-amino ester) (LPAE), poly(5-amino-1-pentanol-co-1,4-butanediol diacrylate) (C32), has demonstrated gene transfection efficiency comparable to viral-mediated gene delivery. Herein, we report the synthesis of a series of highly branched poly(5-amino-1-pentanol-co-1,4-butanediol diacrylate) (HC32) and explore how the branching structure influences the performance of C32 in gene transfection. HC32 were synthesized by an “A2 + B3 + C2” Michal addition strategy. Gaussia luciferase (Gluciferase) and green fluorescent protein (GFP) coding plasmid DNA were used as reporter genes and the gene transfection efficiency was evaluated in human cervical cancer cell line (HeLa) and human recessive dystrophic epidermolysis bullosa keratinocyte (RDEBK) cells. We …

Surface Attachment Of Active Antimicrobial Coatings Onto Conventional Plastic Based Laminates And Performance Assessment Of These Materials On The Storage Life Of Vacuum Packaged Beef Sub-Primals, D. Clarke, A. Tyuftin, M. Cruz-Romero, D. Bolton, S. Fanning, S. K. Pankai, Carmen Bueno-Ferrer, Patrick J. Cullen, J. P. Kerry

Articles

Two antimicrobial coatings, namely Sodium octanoate and Auranta FV (a commercial antimicrobial composed of bioflavonoids, citric, malic, lactic, and caprylic acids) were used. These two antimicrobials were surface coated onto the inner polyethylene layer of cold plasma treated polyamide films using beef gelatin as a carrier and coating polymer. This packaging material was then used to vacuum pack beef sub-primal cuts and stored at 4 °C. A control was prepared using the non-coated commercial laminate and the same vacuum packaged sub-primal beef cuts. During storage, microbial and quality assessments were carried out. Sodium octanoate treated packages significantly (p < 0.05) reduced microbial counts for all bacteria tested with an increase of 7 and 14 days, respectively compared to control samples. No significant effect on pH was observed with any treatment. The results suggested that these food grade antimicrobials have the potential to be used in antimicrobial active packaging applications for beef products.

Achieving Reactive Species Specificity Within Plasma Activated Water Through Selective Generation Using Air Spark And Glow Discharges, Peng Lu, Daniela Boehm, Paula Bourke, Patrick J. Cullen

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Plasma activated liquids (PAL) attract increasing interest with demonstrated biological effects. Plasma exposure in air produces stable aqueous reactive species which can serve as chemical diagnostics of PAL systems. Here, we tailor aqueous reactive species inside plasma activated water (PAW) through treating water with AC air spark and glow discharges in contact with water. Chemical probing demonstrated species specificity between two types of PAW. Spark discharge PAW contains 2 2 H O and 3 NO , while 2 NO and 3 NO are generated in glow discharge PAW. Species formation in different PAWs have been discussed in terms of discharge …

Evaluation Of Chemical Immersion Treatments To Reduce Microbial Populations In Fresh Beef, Ahmed Kassem, Joseph Meade, James Gibbons, Kevina Mcgill, Ciara Walsh, James Lyng, Paul Whyte

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The aim of the current study was to assess the ability of a number of chemicals (acetic Acid (AA), citric acid (CA) lactic acid (LA), sodium decanoate (SD) and trisodium phosphate (TSP)) to reduce microbial populations (total viable count, Campylobacter jejuni, Escherichia coli, Salmonella typhimurium and Listeria monocytogenes) on raw beef using an immersion system. The following concentrations of each chemical were used: 3 & 5% for AA, CA, LA, SD and 10 & 12% for TSP. Possible synergistic effects of using combinations of two chemicals sequentially (LA +CA and LA +AA) were also investigated. L*, a* and b* values …

The Potential Of Atmospheric Air Cold Plasma For Control Of Bacterial Contaminants Relevant To Cereal Grain Production, Agata Los, Dana Ziuzina, Daniela Boehm, Patrick J. Cullen, Paula Bourke

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The aim of this work was to investigate the efficacy of dielectric barrier discharge atmospheric cold plasma (DBD ACP) against bacteria associated with grains quality and safety. ACP inactivation efficacy was tested against biofilms formed by different strains of E. coli, Bacillus and Lactobacillus in grain model media and against B. atrophaeus endospores either in grain media or attached on abiotic surfaces. Effects were dependent on bacterial strain, media composition and mode of ACP exposure. ACP treatment for 5min reduced E. coli spp., B. subtilis and Lactobacillus spp. biofilms by >3 log10, whereas insignificant reductions were achieved for B. atrophaeus. …

Efficacy And Mechanistic Insights Into Endocrine Disruptor Degradation Using Atmospheric Air Plasma, Chaitanya Sarangapani, M. Danaher, Brijesh Tiwari, Peng Lu, Paula Bourke, Patrick J. Cullen

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Endocrine disruptors are a class of contaminants found in water and process effluents at low concentrations. They are of concern due to their high estrogenic potency. Their presence in the environment has led to the search for effective techniques for their removal in wastewater. For this purpose, an atmospheric air plasma reactor was employed for the study of the degradation of three endocrine disruptor chemicals (EDC) namely; bisphenol A (BPA), estrone (E1) and 17β-estradiol (E2) within a model dairy effluent. Identification of the plasma induced active species both in the gas and liquid phases were performed. Also studied was the …

The Application Of Carbon Monoxide In Meat Packaging Needs To Be Re-Evaluated Within The Eu: An Overview, Lauren Anne Van Rooyen, Paul Allen, David I. O'Connor

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Carbon monoxide (CO) has many value-added benefits in meat packaging due to its colour stabilising effects and enhancement of meat quality attributes. The regulation of CO within meat packaging varies worldwide and remains a topical and controversial issue. CO is prohibited in the EU for use in meat packaging mainly due to fears it may mask spoilage therefore misleading consumers. The issue of consumer acceptance of CO was not considered. This article reviews the most pertinent literature to assess if the problems associated with the prohibition have been addressed. Applying CO pretreatments prior to vacuum packaging enhances colour while allowing …

"Then To Death Walked, Softly Smiling": Violence And Martyrdom In Modern Irish Republican Ballads, Seán Ó Cadhla

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This article critically considers the representation of death within the song tradition of modern Irish Republicanism. I explore how such representations have changed in parallel with the various ideological metamorphoses that Irish Republicanism has undergone, specifically in the twentieth century. I argue that the centrality of self-sacrifice has resulted in the development of ballad narratives that deliberately obfuscate on the issue of Republican violence, resulting in the deaths of all Republican militants (regardless of cause or context), ultimately portrayed as a form of heroic self-martyrdom.

San alt seo, déantar anailís chriticiúil ar léiriú an bháis i dtraidisiún amhránaíocht Phoblachtach na …

In Vitro Comparative Cytotoxicity Study Of Aminated Polystyrene, Zinc Oxide And Silver Nanoparticles On A Cervical Cancer Cell Line, Akash Sharma, Brian Gorey, Alan Casey

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Nanoparticles use in nano-biotechnology applications have increased significantly with Aminated polystyrene amine (AmPs NP), Zinc oxide (ZnO NP), and Silver (Ag NP) nanoparticles utilized in wide variety of consumer products. This has presented a number of concerns due to their increased exposure risks and associated toxicity on living systems. Changes in the structural and physicochemical properties of nanoparticles can lead to changes in biological activities. This study investigates, compares, and contrasts the potential toxicity of AmPs, ZnO and Ag NPs on an in vitro model (HeLa cells) and assesses the associated mechanism for their corresponding cytotoxicity relative to the surface …

Carbon Nanomaterials And Their Application To Electrochemical Sensors: A Review, Aoife Power, Shaneel Chandra, Brian Gorey, James Chapman

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Carbon has long been applied as an electrochemical sensing interface owing to its unique electrochemical properties. Moreover, recent advances in material design and synthesis, particularly nanomaterials, has produced robust electrochemical sensing systems that display superior analytical performance. Carbon nanotubes (CNTs) are one of the most extensively studied nanostructures because of their unique properties. In terms of electroanalysis, the ability of CNTs to augment the electrochemical reactivity of important biomolecules and promote electron transfer reactions of proteins is of particular interest. The remarkable sensitivity of CNTs to changes in surface conductivity due to the presence of adsorbates permits their application as …

Biofunctionalized Capillary Flow Channel Platform Integrated With 3d Nanostructured Matrix To Capture Circulating Tumor Cells, Shashwat Banerjee, Ganesh Khutale, Vrushali Khobragade, Narendra Kale, Milind Pore, Govind Chate, Archana Jalota-Badhwar, Manoj Dongare, Jayant Khandare

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Circulating tumor cells (CTCs) from peripheral blood account genetic information for cancer diagnosis and overall disease monitoring. Analysis of “liquid biopsy” holds immense promise as it may lead to new approaches for cancer treatment. The study reports effective and continuous flow microchannel system for isolating CTCs using transferrin conjugated 3D matrix synthesized by crosslinking polyethylene glycol-Fe3O4 nanostructures for rapid and efficient capturing of CTCs. The platform provides option of using multiple microchannel units in series that can influence higher cell-capture efficiency due to increasing cell-substrate contact frequency. CTCs are captured with high efficiency even at low concentration of target cells …