Digital Commons Network^™

Simple Polystyrene Microfluidic Device For Sensitive And Accurate Sers-Based Detection Of Infection By Malaria Parasites, Maria Joao Oliveira, Soraia Caetano, Ana Dalot, Filipe Sabino, Tomas Calmeiro, Elvira Fortunato, Rodrigo Martins, Eulalia Pereira, Miguel Prudencio, Hugh Byrne, Ricardo Franco, Hugo Aguas

Articles

Early and accurate detection of infection by pathogenic microorganisms, such as Plasmodium, the causative agent of malaria, is critical for clinical diagnosis and ultimately determines the patient’s outcome. We have combined a polystyrene-based microfluidic device with an immunoassay which utilises Surface-Enhanced Raman Spectroscopy (SERS) to detect malaria. The method can be easily translated to a point-of-care testing format and shows excellent sensitivity and specificity, when compared to the gold standard for laboratorial detection of Plasmodium infections. The device can be fabricated in less than 30 min by direct patterning on shrinkable polystyrene sheets of adaptable three-dimensional microfluidic chips. To validate …

Multivariate Curve Resolution-Alternating Least Squares Coupled With Raman Microspectroscopy: New Insights Into The Kinetic Response Of Primary Oral Squamous Carcinoma Cells To Cisplatin, Valentina Notarstefano, Alessia Belloni, Paolo Mariani, Giulia Orilisi, Giovanna Orsini, Elisabetta Giorgini, Hugh Byrne

Articles

Raman MicroSpectroscopy (RMS) is a powerful label-free tool to probe the effects of drugs at a cellular/ subcellular level. It is important, however, to be able to extract relevant biochemical and kinetic spectroscopic signatures of the specific cellular responses. In the present study, a combination of Multivariate Curve Resolution-Alternating Least Squares (MCR-ALS) and Principal Component Analysis (PCA) is used to analyse the RMS data for the example of exposure of primary Oral Squamous Carcinoma Cells (OSCC) to the chemotherapeutic agent cisplatin. Dosing regimens were established by cytotoxicity assays, and the effects of the drug on cellular spectral profiles were monitored …

Laser Light As An Emerging Method For Sustainable Food Processing, Packaging, And Testing, Prasad Chavan, Rahul Yadav, Pallavi Sharma, Amit Jaiswal

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In this review article, we systematically investigated the diverse applications of laser technology within the sphere of food processing, encompassing techniques such as laser ablation, microbial inactivation, state-of-the-art food packaging, and non-destructive testing. With a detailed exploration, we assess the utility of laser ablation for the removal of surface contaminants from foodstuffs, while also noting the potential financial and safety implications of its implementation on an industrial scale. Microbial inactivation by laser shows promise for reducing the microbial load on food surfaces, although concerns have been raised about potential damage to the physio characteristics of some fruits. Laser-based packaging techniques, …

Effect Of Ultrasound-Assisted Thin Bed Drying For Retaining The Quality Of Red Bell Pepper And Compare The Predictive Ability Of The Mathematical Model With Artificial Neural Network, Raju Sasikumar, Irengbam Barun Mangang, Kamchampati Vivek, Amit K. Jaiswal

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Red bell peppers (Capsicum ann. L.) are low in calories and high in nutrients, including vitamins A and C. Various factors such as weight loss, senescence, and microbial influence affect their quality during storage. To address this, the present study aims to preserve and retain the quality of red bell peppers using ultrasound-assisted thin-bed drying. The results were analyzed using an Artificial Neural Network (ANN) for more accurate prediction of variables involved in the process. Variations in moisture ratio and moisture content during drying were calculated and predicted. The Mid. model provided satisfactory curve fitting at an air velocity of …

Electromechanical Coupling In Collagen Measured Under Increasing Relative Humidity, Arwa Bazaid, Fengyuan Zhang, Qiancheng Zhang, Sabine Neumayer, Denise Denning, Stefan Habelitz, Ana Marina Ferreira, Brian J. Rodriguez

Articles

The functional role of collagen piezoelectricity has been under debate since the discovery of piezoelectricity in bone in 1957. The possibility that piezoelectricity plays a role in bone remodelling has generated interest in the investigation of this effect in relevant physiological conditions; however, there are conflicting reports as to whether collagen is piezoelectric in a humid environment. In macroscale measurements, the piezoelectricity in hydrated tendon has been shown to be insignificant compared to dehydrated tendon, whereas, at the nanoscale, the piezoelectric effect has been observed in both dry and wet bone using piezo response force microscopy (PFM). In this work, …

Label-Free Quantification Of Nanoencapsulated Piperonyl Esters In Cosmetic Hydrogels Using Raman Spectroscopy, Suha Elderderi, Franck Bonnier, Xavier Perse, Hugh Byrne, Florent Yvergnaux, Igor Chourpa, Abdalla A. Elbashir, Emilie Munnier

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Raman spectroscopy is a well-established technique for the molecular characterisation of samples and does not require extensive pre-analytical processing for complex cosmetic products. As an illustration of its potential, this study investigates the quantitative performance of Raman spectroscopy coupled with partial least squares regression (PLSR) for the analysis of Alginate nanoencapsulated Piperonyl Esters (ANC-PE) incorporated into a hydrogel. A total of 96 ANC-PE samples covering a 0.4% w/w–8.3% w/w PE concentration range have been prepared and analysed. Despite the complex formulation of the sample, the spectral features of the PE can be detected and used to quantify the concentrations. Using …

Influence Of Physical And Mechanical Parameters On Cavitation Erosion And Antifouling Behaviour Of Multilayer Silica-Based Hybrid Sol–Gel Coatings On Aluminium Alloys, Manasa Hegde, Marta Mroczkowska, Joseph Mohan, Adriana Cunha Neves, Yvonne Kavanagh, Brendan Duffy, Edmond F. Tobin

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Sol–gel coatings can provide anti-fouling and erosion resistance while being safe to use in the marine environment. MAPTMS/ZPO multilayer coatings deposited on the AA2024-T3 aluminium surface using the dip-coating method at three different thicknesses (2, 4, and 6 m) are investigated in this work. The coatings are characterised in terms of physical and mechanical properties, and these properties are investigated in comparison to previously obtained cavitation erosion resistance levels of the coatings. Additionally, the efficiency of the coatings against biofouling was assessed using Phaeodactylum tricornutum, a marine diatom. The influence of the formation of organic–inorganic hybrid materials (OIHMs) from the …

Investing In Virtue And Frowning At Vice? Lessons From The Global Economic And Financial Crisis, Lucia Morales, Daniel Rajmil

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Socially responsible mutual funds (SRMF) and the “antisocially conscious”, Vitium Global Fund Barrier Fund (formerly known as the Vice Fund, the term used in this paper) returns, volatility patterns, and causal effects are examined in this study within the context of the lessons learned from the 2008 Global Economic and Financial Crisis (GEFC). In times of a new and unprecedented crisis due to the COVID-19 pandemic, a look back to our recent past reveals that volatility patterns on daily stock returns presented some level of predictability on prices for both types of funds. The research findings are significant as funds’ …

Towards An Inclusive And Representative Academic Landscape, Linn Leppert, Katalin Solymosi, Yvonne Galligan

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This article is a summary of a panel discussion entitled ‘Towards an inclusive and representative academic landscape’, held at the Building Bridges Meeting of Academia Europaea and the Young Academy of Europe on 26 October 2022. The panellists were Professor Yvonne Galligan, director of Equality, Diversity and Inclusion and Professor of Comparative Politics at the Technological University Dublin, Dr Katalin Solymosi, plant biologist, assistant professor at Eötvös Loránd University and vice-chair of the Young Academy of Europe, and Professor Stephen Curry, Assistant Provost for Equality, Diversity and Inclusion and Professor of Structural Biology at Imperial College London. Dr Linn Leppert, …

Estimate Tourism Model Choice For Pilgrim In The Way Of St. James: The Portuguese Way, Rosa Conde, Ana Pinto Borges, Isabel Neira Gómez, Elvira Viera

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The purpose of this study is to examine how the characteristics of the pilgrims and the attributes of travel influence the choose of the Portuguese. It is important to understand the choices tourists make. This study identifies the factors that influence the tourists’ choice of way. The analysis is based on the official statistics of the Portuguese Way of Santiago between 2003 and 2020.

The study provides several important findings concerning tourism’s model choice. Understanding the tourists’ modal choice behavior help public and private organizations to develop appropriate marketing strategies.

The probit model is used to model a relationship between …

The Future Of Clinical Trials Of Myopia Control, Mark A. Bullimore, Noel A. Brennan, Daniel I. Flintcroft

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In the field of myopia control, effective optical or pharmaceutical therapies are now available to patients in many markets. This creates challenges for the conduct of placebo-controlled, randomised clinical trials, including ethics, recruitment, retention, selective loss of faster progressors and non-protocol treatments: 1. Ethics: It is valid to question whether withholding treatment in control subjects is ethical. 2. Recruitment: Availability of treatments is making recruitment into clinical trials more difficult. 3. Retention: If masking is not possible, parents may immediately withdraw their child if randomised to no treatment. 4. Selective loss: Withdrawal of fast progressors in the control group leading …

“Mind The Gap” - An Overview Of The Role Of The Extensions Community Healthcare Outcomes (Echo) Model In Enhancing Value In Health Care Delivery, Christina Kenny, Anushree Priyadarshini

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The ECHO (Extensions Community Healthcare Outcomes) model of healthcare delivery has grown rapidly since its establishment and increased in popularity in recent years. This expansion has developed alongside the growing incidence of chronic diseases and the need to better manage them. The increasing uptake in ECHO has presented a requirement to assess its true value as healthcare costs are increasing globally, resulting in a growing demand by governments and policy makers to ensure chronic disease management strategies provide true value. Therefore, the aim of this review is to examine the impact that ECHO has on clinical practice and how such …

Serum 25-Hydroxyvitamin D Response To Vitamin D Supplementation Using Different Lipid Delivery Systems In Middle-Aged And Older Adults: A Randomised Controlled Trial, Aisling F. Mccourt, Steven L. Mulrooney, Graham O'Neill, E. Dolores O'Riordan, Aifric M. O'Sullivan

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Food fortification improves vitamin D intakes but is not yet mandated in many countries. Combining vitamin D with different dietary lipids altered vitamin D absorption in in vitro and postprandial studies. This randomised, placebo-controlled trial examined the effect of the lipid composition of a vitamin D-fortified dairy drink on change in 25-hydroxyvitamin D (25(OH)D) concentrations. Sixty-three healthy adults aged 50+ years were randomised to one of the following for 4 weeks: vitamin D-fortified olive oil dairy drink, vitamin D-fortified coconut oil dairy drink, vitamin D supplement or placebo control dairy drink. All vitamin D groups received 20 µg of vitamin …

Enhanced Fractional Adaptive Processing Paradigm For Power Signal Estimation, Naveed Ishtiaq Chaudhary, Zeshan Aslam Khan, Muhammad Asif Zahoor Raja, Iqra Ishtiaq Chaudhary

Articles

Fractional calculus tools have been exploited to effectively model variety of engineering, physics and applied sciences problems. The concept of fractional derivative has been incorporated in the optimization process of least mean square (LMS) iterative adaptive method. This study exploits the recently introduced enhanced fractional derivative based LMS (EFDLMS) for parameter estimation of power signal formed by the combination of different sinusoids. The EFDLMS addresses the issue of fractional extreme points and provides faster convergence speed. The performance of EFDLMS is evaluated in detail by taking different levels of noise in the composite sinusoidal signal as well as considering various …

Three-Dimensional (3d) In Vitro Cell Culture Protocols To Enhance Glioblastoma Research, Janith Wanigasekara, Lara Carroll, Patrick J. Cullen, Brijesh K. Tiwari, James Curtin

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Three-dimensional (3D) cell culture models can help bridge the gap between in vitro cell cultures and in vivo responses by more accurately simulating the natural in vivo environment, shape, tissue stiffness, stressors, gradients and cellular response while avoiding the costs and ethical concerns associated with animal models. The inclusion of the third dimension in 3D cell culture influences the spatial organization of cell surface receptors that interact with other cells and imposes physical restrictions on cells in compared to Two-dimensional (2D) cell cultures. Spheroids’ distinctive cyto-architecture mimics in vivo cellular structure, gene expression, metabolism, proliferation, oxygenation, nutrition absorption, waste excretion, …

Antibacterial And Antifungal Activity Of Methanolic Extracts Of Salix Alba L. Against Various Disease Causing Pathogens, Bilal Javed, F. Farooq, M. Ibrahim, H.A.B. Abbas, H. Jawwad, S.S. Zehra, H.M. Ahmad, A. Sarwar, K. Malik, K. Nawaz

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The present study was aimed to manifest the antibacterial and antifungal activity of methanolic extracts of Salix alba L. against seven Gram-positive and Gram-negative bacterial pathogens e.g. Streptococcus pyogenes, Staphylococcus aureus (1), S. aureus (2), Shigella sonnei, Escherichia coli (1), E. coli (2) and Neisseria gonorrhoeae and three fungal isolates from the air such as Aspergillus terreus, A. ornatus, and Rhizopus stolonifer. Two different serotypes of S. aureus and E. coli were used. The agar well-diffusion method results showed the dose-dependent response of plant extracts against bacterial and fungal strains while some organisms were found resistant e.g. E. coli (1), …

Design, Synthesis And Biological Evaluation Of A Novel Bioactive Indane Scaffold 2-(Diphenylmethylene)C-2,3-Dihydro-1h-Inden-1-One With Potential Anticancer Activity, Tao Zhang, Vilmar Bandero, Claire Corcoran, Ismael Obaidi, Manuel Ruether, John O'Brien, Lorraine O'Driscoll, Neil Frankish, Helen Sheridan

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Over the past decades, designing of privileged structures has emerged as a useful approach to the discovery and optimisation of novel biologically active molecules, and many have been successfully exploited across and within different target families. Examples include indole, quinolone, isoquinoline, benzofuran and chromone, etc. In the current study, we focus on synthesising a novel hybrid scaffold constituting naturally occurring benzophenone (14) and indanone (22) ring systems, leading to a general structure of 2-(diphenylmethylene)-2,3-dihydro-1H-inden-1-one (23). It was hypothesised this new hybrid system would provide enhanced anti-cancer activity owing to the presence of the common features associated with the tubulin binding …

Numeracy-Meets: An Innovative Professional Development Model For Adult Numeracy Practitioners In Ireland, Mark Prendergast, Annette Forster, Niamh O'Meara, Kathy O'Sullivan, Fiona Faulkner

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Despite the clear and obvious need for adults to be proficient in numeracy, international studies suggest that many continue to struggle in this area. In Ireland, one of the main challenges continues to be the availability of effective adult numeracy education. This is a diverse sector, and little is known about the varied provision of adult numeracy courses and of those who teach on them. Recent research has highlighted an unmet demand for the professional development of adult numeracy practitioners with many looking for opportunities to network and further develop their practice. This study aimed to design, implement and evaluate …

Assessing Authentically – Learnings From Marketing Educators, S. Montano, L. Gill-Simmen, D. Lee, Lucia Walsh, Deirdre Duffy Dr, N. Newman

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This paper demonstrates the importance and role of authentic assessments, that replicate industry practice, within Higher Education marketing programmes. We answer the call from employers, that students need to gain much-needed employability skills, and we illustrate how such assessments can be created to enable the development of employability skills. We provide an overview of four case studies, to illustrate different forms of authentic assessments, the theory which was used to underpin the designs, the skills developed during the assessments, and the outcomes of the assessments. As we emerge from the COVID-19 pandemic, which forced a move to online assessments, it …

Machine-Learning-Based Head Impact Subtyping Based On The Spectral Densities Of The Measurable Head Kinematics, Xianghao Zhan, Yiheng Li, Yuzhe Liu, Nicholas J. Cecchi, Samuel J. Raymond, Zhou Zhou, Hossein Vahid Alizadeh, Jesse Ruan, Saeed Barbat, Stephen Tiernan, Olivier Gevaert, Michael M. Zeineh, Gerald A. Grant, David B. Camarillo

Articles

Traumatic brain injury can be caused by head impacts, but many brain injury risk estimation models are not equally accurate across the variety of impacts that patients may undergo and the characteristics of different types of impacts are not well studied. We investigated the spectral characteristics of different head impact types with kinematics classification. Methods: Data was analyzed from 3,262 head impacts from lab reconstruction, American football, mixed martial arts, and publicly available car crash data. A random forest classifier with spectral densities of linear acceleration and angular velocity was built to classify head impact types (e.g., football, car crash, …

Tio2-Mwcnt Nanohybrid: Cytotoxicity, Protein Corona Formation And Cellular Internalisation In Rtg-2 Fish Cell Line, Gabriela Helena Da Silva, Lidiane Silva Franqui, Marcelo A. De Farias, Vera Lucia S.S. De Castro, Hugh Byrne, Diego S.T. Martinez, Regina T.R. Monteiro, Alan Casey

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Titanium dioxide nanoparticles-multiwalled carbon nanotubes (TiO2-MWCNT) nanohydrid has an enhanced photocatalytic activity across the visible light with promising applications in environmental remediation, solar energy devices and antimicrobial technologies. However, it is necessary to evaluate the toxicological effects of TiO2-MWCNT towards safe and sustainable development of nanohybrids. In this work, we studied the cytotoxicity, protein corona formation and cellular internalisation of TiO2-MWCNT on fibroblasts derived from gonadal rainbow trout tissue (RTG-2) for the first time. This nanohydrid did not show any toxicity effect on RTG-2 cells up to 100 mg L-1 after 24 h of exposure as monitored by alamar blue, …

Know An Emotion By The Company It Keeps: Word Embeddings From Reddit/Coronavirus, Alejandro García-Rudolph, David Sanchez-Pinsach, Dietmar Frey, Eloy Opisso, Katryna Cisek, John Kelleher

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Social media is a crucial communication tool (e.g., with 430 million monthly active users in online forums such as Reddit), being an objective of Natural Language Processing (NLP) techniques. One of them (word embeddings) is based on the quotation, “You shall know a word by the company it keeps,” highlighting the importance of context in NLP. Meanwhile, “Context is everything in Emotion Research.” Therefore, we aimed to train a model (W2V) for generating word associations (also known as embeddings) using a popular Coronavirus Reddit forum, validate them using public evidence and apply them to the discovery of context for specific …

Rapid Detection Of Sars-Cov-2 Nucleocapsid Protein By A Label-Free Biosensor Based On Optical Fiber Cylindrical Micro-Resonator, Ru-Lei Xia, Bin Liu, Yingying Hu, Juan Liu, Yue Fu, Xing-Dao He, Ping Lu, Yingying Hu, Gerald Farrell

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The current global outbreak of coronavirus (COVID-19) continues to be a severe threat to human health. Rapid, low-cost, and accurate antigen detection methods are very important for disease diagnosis. The severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) nucleocapsid protein (N-Protein) is often used as the diagnostic and screening for coronavirus detection. To this end, we propose and experimentally validate a highly sensitive whispering gallery mode (WGM) optical cylindrical micro-resonator (CMR) for bio immunoassay detection. To study the biokinetic process of immunoassay, the surface of the WGM micro-resonator is functionalized with N-Protein monoclonal antibody (N-Protein-m Ab), which led to the specific detection …

Characterization Of Food Chain Clostridioides Difficile Isolates In Terms Of Ribotype And Antimicrobial Resistance, Pilar Marcos, Aoife Doyle, Paul Whyte, Thomas R. Rogers, Maire Mcelroy, Seamus Fanning, Jesus Maria Frias, Declan Bolton

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The aim of this study was to characterize C. difficile isolates from the farm, abattoir, and retail outlets in Ireland in terms of ribotype and antibiotic resistance (vancomycin, erythromycin, metronidazole, moxifloxacin, clindamycin, and rifampicin) using PCR and E-test methods, respectively. The most common ribotype in all stages of the food chain (including retail foods) was 078 and a variant (RT078/4). Less commonly reported (014/0, 002/1, 049, and 205) and novel (RT530, 547, and 683) ribotypes were also detected, but at lower frequencies. Approximately 72% (26/36 tested) of the isolates tested were resistant to at least one antibiotic, with the majority …

Hmm, You Seem Confused! Tracking Interlocutor Confusion For Situated Task-Oriented Hri, Na Li, Robert J. Ross

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Our research seeks to develop a long-lasting and high-quality en- gagement between the user and the social robot, which in turn requires a more sophisticated alignment of the user and the system than is currently commonly available. Close monitoring of inter- locutors’ states, and we argue their confusion state in particular, and adjusting dialogue policies based on this state of confusion is needed for successful joint activity. In this paper, we present an ini- tial study of a human-robot conversation scenarios using a Pepper robot to investigate the confusion states of users. A Wizard-of-Oz (WoZ) HRI experiment is illustrated in …

International Digital Nomads: Immigration Law Options In The United States And Abroad, Scott Titshaw

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Remote work has become common, allowing many people to choose to work anywhere with an adequate internet connection. Some are adopting a “digital nomad” lifestyle, moving with the seasons or years from place to place, including foreign locations. Yet, such international movement raises immigration and other legal issues. Many countries have adopted specific digital nomad visas and other immigration policies to encourage and regulate this trend. The United States is not one of them. Arguing that the United States should consciously plan for digital nomads, this article compares the current U.S. approach with the innovations of other countries, identifying the …

Stories That Kill: Masculinity And Capital Prosecutors' Closing Arguments, Pamela A. Wilkins

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The American death penalty is a punishment by, for, and about men: Both historically and today, most capital prosecutors are men, most capital defendants are men, and killing itself is strongly coded male. Yet despite—or perhaps because of—the overwhelming maleness of the institution of capital punishment, the subject of masculinity is largely absent from legal discourse about the death penalty. This Article addresses that gap in the legal discourse by applying the insights of masculinities theory, an offshoot of feminist theory, to capital prosecutors’ closing arguments. This Article hypothesizes that capital prosecutors’ masculinity is strongly influenced both by white Southern …

Ar/Vr Teaching-Learning Experiences In Higher Education Institutions (Hei): A Systematic Literature Review, Belen Bermejo, Carlos Juiz, David Cortes, Jeroen Oskam, Teemu Moilanen, Jouko Loijas, Praneschen Govender, Jenniifer Hussey, Alexander Lennart Schmidt, Ralf Burbach, Daniel King, Colin O'Connor, David Dunlea

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During the last few years, learning techniques have changed, both in basic education and in higher education. This change has been accompanied by new technologies such as Augmented Reality (AR) and Virtual Reality (AR). The combination of these technologies in education has allowed a greater immersion, positively affecting the learning and teaching processes. In addition, since the COVID-19 pandemic, this trend has been growing due to the diversity of the different fields of application of these technologies, such as heterogeneity in their combination and their different experiences. It is necessary to review the state of the art to determine the …

Experiencing Dyslexia Through The Prism Of Difference, Keith Murphy

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According to research by AHEAD (2021), students with specific learning difficulties (SLD) are accessing third level education in greater numbers than ever before. Within the body of research conducted few studies have focused on the overall experiences of students with dyslexia studying in third level education. The current study addresses this gap in knowledge as it provides an insight into how students with dyslexia, as an SLD, navigate third level education. Ethnography was used as the principal method of research in this project, and 17 participants, ranging in age from 20 years old to mid-40 years old, took part. The …

Virtual Industry Visits, Cian Farrell

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Cian Farrell of Technological University Dublin describes a virtual educational approach trialled during the Covid-19 pandemic to engage students during a period of remote learning and educate them on industry processes and practices. Virtual industry visits saw students receive online briefings and tours of manufacturing plants and construction sites from industry representatives to complement their taught modules. The virtual visits have continued since Covid restrictions eased as part of a hybrid approach to learning that increases student exposure to real-life projects. The initiative won the Institution of Structural Engineers Excellence in Structural Engineering Education Award in 2022.