Digital Commons Network^™

Size Dependent Translocation And Fetal Accumulation Of Gold Nanoparticles From Maternal Blood In The Rat, Manuela Semmler-Behnke, Jens Lipka, Alexander Wenk, Martin Schaeffer, Furong Tian, Gunter Schmid, Gunter Oberdorster, Wolfgang Kreyling

Articles

There is evidence that nanoparticles (NP) cross epithelial and endothelial body barriers. We hypothesized that gold (Au) NP, once in the blood circulation of pregnant rats, will cross the placental barrier during pregnancy size-dependently and accumulate in the fetal organism by 1. transcellular transport across the hemochorial placenta, 2. transcellular transport across amniotic membranes 3. transport through ~20 nm wide transtrophoblastic channels in a size dependent manner. The three AuNP sizes used to test this hypothesis are either well below, or of similar size or well above the diameters of the transtrophoblastic channels.

Disability, Repressive Regimes, And Health Disparity: Assessing Country Conditions In North Korea, Jae-Chun Won, Janet E. Lord, Michael Ashley Stein, Yosung Song

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This article examines the potential of international human rights standards to account for heightened risk and vulnerability to human rights abuses for persons with disabilities in politically repressive environments. We offer a preliminary assessment of whether and how a disability-cognizant accounting of human rights in repressive regimes can lead to a more credible and comprehensive understanding of disability in such contexts. In so doing, we make the case for a more thorough and thoughtful application of international disability rights standards to better inform human rights investigations, transitional justice approaches, and international criminal law proceedings. To illustrate this methodology, we analyze …

The Democratic Life Of The Union: Toward Equal Voting Participation For Europeans With Disabilities, Janos Fiala-Butora, Michael Ashley Stein, Janet E. Lord

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This Article puts forward preliminary legal scholarship on equal political participation by persons with disabilities and what international human rights law requires for its attainment. The goal is to provoke an informed dialogue on the neglected but fundamental human right to enfranchisement by persons with disabilities while also acknowledging that a complete and just resolution requires further information and reflection. The Article argues that the fundamental right to vote cannot be curtailed on the basis of an alleged lack of capacity. Disenfranchisement based on individual assessment unjustly excludes a certain number of voting-capable individuals. Since all those affected are persons …

Mechanism Of Multiple Grating Formation In High-Energy Recording Of Holographic Sensors, Ali K. Yetisen, Yunuen Montelongo, Nicholas M. Farandos, Izabela Naydenova, Christopher R. Lowe, Seok Hyun Yun

Articles

We report numerical analyses of Bragg diffraction by Denisyuk reflection holograms recorded by a high-energy pulsed laser. An intensity threshold must be passed to pattern a multilayer reflection and transmission hologram, which exhibits a nonlinear fringe structure. Numerical evaluations are provided for the laser light intensity, readout diffraction offset angle, transmission of the layer, and thickness of the polymer matrix during hologram recording. A non-sinusoidal surface pattern is formed at the top of the multilayer structure, and its effect on the diffraction properties of the structure becomes significant when the recording tilt angle is increased. Experimental results show that the …

Why The Buffett-Gates Giving Pledge Requires Limitation Of The Estate Tax Charitable Deduction, Edward A. Zelinsky

Articles

The Buffett-Gates Giving Pledge, under which wealthy individuals promise to leave a majority of their assets to charity, is an admirable effort to encourage philanthropy. However, the Pledge requires us to confront the paradox that the federal estate tax charitable deduction is unlimited while the federal income tax charitable deduction is capped. If a Giving Pledger leaves his wealth to charity, the federal fisc loses significant revenue since the Pledger thereby avoids federal estate taxation as charitable bequests are deductible without limit for federal estate tax purposes. Despite its laudable qualities, the Giving Pledge is a systematic (albeit inadvertent) threat …

Shaken Baby Syndrome On Trial, Keith A. Findley, Barry Scheck

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This is a short review of Deborah Tuerkheimer's book, "Flawed Convictions: 'Shaken Baby Syndrome' and the Inertia of Injustice" (Oxford University Press 2014).

Comparison Of Semi-Automated And Manual Measurements Of Carotid Intima-Media Thickening, Oscar Mac Ananey, G Mellotte, V Maher

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Carotid intima-media thickening (CIMT) is a marker of both arteriosclerotic and atherosclerotic risks. Technological advances have semiautomated CIMT image acquisition and quantification. Studies comparing manual and automated methods have yielded conflicting results possibly due to plaque inclusion in measurements. Low atherosclerotic risk subjects (n = 126) were recruited to minimise the effect of focal atherosclerotic lesions on CIMT variability. CIMT was assessed by high-resolution B-mode ultrasound (Philips HDX7E, Phillips, UK) images of the common carotid artery using both manual and semiautomated methods (QLAB, Phillips, UK). Intraclass correlation coefficient (ICC) and the mean differences of paired measurements (Bland-Altman method) were used …

Increased Expression Of Phosphorylated Fadd In Anaplastic Large Cell And Other T-Cell Lymphomas, Suketu Patel, Derek Murphy, Eugenia Haralambieva, Zainalabideen A. Abdulla, Kah Keng Wong, Hong Chen, Edith Gould, Giovanna Roncador, Chris S.R. Hatton, Amanda P. Anderson, Alison H. Banham, Karen Pulford

Articles

FAS-associated protein with death domain (FADD) is a major adaptor protein involved in extrinsic apoptosis, embryogenesis, and lymphocyte homeostasis. Although abnormalities of the FADD/death receptor apoptotic pathways have been established in tumorigenesis, fewer studies have analyzed the expression and role of phosphorylated FADD (pFADD). Our identification of FADD as a lymphoma-associated autoantigen in T-cell lymphoma patients raises the possibility that pFADD, with its correlation with cell cycle, may possess role(s) in human T-cell lymphoma development. This immunohistochemical study investigated pFADD protein expression in a range of normal tissues and lymphomas, particularly T-cell lymphomas that require improved therapies. Whereas pFADD was …

Btk Regulated Macrophage Polarization In Response To Lipopolysaccharide, Joan Ní Gabhann, Emily Hams, Siobhán Smith, Claire Wynne, Jennifer C. Byrne, Kiva Brennan, Shaun Spence, Adrien Kissenpfennig

Articles

Bacterial Lipopolysaccharide (LPS) is a strong inducer of inflammation and does so by inducing polarization of macrophages to the classic inflammatory M1 population. Given the role of Btk as a critical signal transducer downstream of TLR4, we investigated its role in M1/M2 induction. In Btk deficient (Btk^−\−) mice we observed markedly reduced recruitment of M1 macrophages following intraperitoneal administration of LPS. Ex vivo analysis demonstrated an impaired ability of Btk^−/− macrophages to polarize into M1 macrophages, instead showing enhanced induction of immunosuppressive M2-associated markers in response to M1 polarizing stimuli, a finding accompanied by reduced phosphorylation …

Pilot Data On Brain-To-Blood Efflux Of B-Amyloid Peptides In Man, Steve Meaney, Maura Heverin, Ingemar Bjorkhem,, Dorotea Religa, John Wahren, Ulf Diczfalusy

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• Alzheimer’s disease (AD) is the most common cause of dementia and affects nearly 40,000 individuals in Ireland. • The b-amyloid peptide (Ab) plays a key role in the pathogenesis of the AD and the presence of Ab plaques in the brain is diagnostic. •The hypothesis posits that Ab deposition is a critical factor in the disease process and that production and clearance of Ab are key drivers of the disease1. •Flux of Ab from the brain is believed to contribute to the overall level of Ab within in brain2 and antibody mediated brain-to-blood efflux has been observed in animal …

Ethnic Variation In Breastfeeding And Complementary Feeding In The Republic Of Ireland, Patricia Castro, Richard Layte, John Kearney

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Early nutrition plays a pivotal role in long-term health. The World Health Organization (WHO) recommends exclusive breastfeeding during the first six months of life, with the gradual introduction of solids after this period. However, studies in the Republic of Ireland (ROI) have shown poor compliance with guidelines. The ROI continues to have one of the lowest breastfeeding rates worldwide. Our objective was to analyse differences in breastfeeding and complimentary feeding behaviours between Irish and non-Irish mothers residing in the ROI, as well as the role of acculturation on these behaviours, using the national longitudinal study, Growing Up in Ireland (GUI). …

Trim68 Negatively Regulates Ifn-B Production By Degrading Trk Fused Gene, A Novel Driver Of Ifn-B Downstream Of Anti-Viral Detection Systems, Claire Wynne, Elisa Lazzari, Siobhan Smith, Eoghan Mccarthy, Joan Ni Gabhann, Lara Kallai, Rowan Higgs, Sally Ann Cryan, Christine Byron, Caroline Jefferies

Articles

In recent years members of the tripartite motif-containing (TRIM) family of E3 ubiquitin ligases have been shown to both positively and negatively regulate viral defence and as such are emerging as compelling targets for modulating the anti-viral immune response. In this study we identify TRIM68, a close homologue of TRIM21, as a novel regulator of Toll-like receptor (TLR)- and RIG-I-like receptor (RLR)-driven type I IFN production. Proteomic analysis of TRIM68-containing complexes identified TRK-fused gene (TFG) as a potential TRIM68 target. Overexpression of TRIM68 and TFG confirmed their ability to associate, with TLR3 stimulation appearing to enhance the interaction. TFG is …

Getting Started With Research " Beginning: Defining A Research Question And Preparing A Research Plan, Jacinta Browne

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Research is the lifeblood of medicine with innovations being made in the different technologies used to diagnose and / or screen for the presence of disease or to deliver treatments with higher efficacy. Scientific publication is the network of vessels which delivers the lifeblood. The outputs of research provides us with tomorrows medicine. In this series of articles we will be discussing the different aspects of carrying out research from the conception of the research idea to publication in a peer reviewed journal with the cycle repeating again with new research ideas emerging from the research conducted.

Investigation Of The Sensitivity To Humidity Of An Acrylamide-Based Photopolymer Containing N-Phenylglycine As A Photoinitiator, Tatsiana Mikulchyk, Suzanne Martin, Izabela Naydenova

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Sensitivity of holographic recording materials to the relative humidity (RH) of the environment often restricts their use in fabrication of holographic optical elements and other applications. It is important to develop materials with little or no sensitivity to humidity. In this paper the humidity response of transmission gratings recorded in an acrylamide-based photopolymer containing N-phenylglycine (NPG) as a photoinitiator is studied at RH = 20 – 90 %. The hologram is found to be completely insensitive to humidity at RH below 70 % and its diffraction efficiency remains constant. A decrease in diffraction efficiency is observed at RH = 80 …

Holographic Sensors: Three-Dimensional Analyte-Sensitive Nanostructures And Their Applications, Ali K. Yetisen, Izabela Naydenova, Fernando Da Cruz Vasconcellos, Jeffrey Blyth, Christopher R. R. Lowe

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Holographic sensors are analytical devices that systematically diffract narrow-band light in the ultraviolet to near-infrared range for application in the detection and quantification of analytes and/or physical parameters. They can be functionalized with analyte-responsive materials to construct highly sensitive optical sensors for use in testing, where a visual readout, fast turnaround time, and reversibility are needed. Holography allows fabrication of disposable sensors that are lightweight for miniaturization and multiplexing purposes.3 Holographic sensors offer three capabilities on a single analytical device: (i) label-free analyte-responsive polymer, (ii) real-time, reversible quantification of the external stimuli, and (iii) three-dimensional visual image display.

Socioeconomic Differentials In The Immediate Mortality Effects Of The National Irish Smoking Ban, Patrick J. Goodman, Sericea Stallings-Smith, Ariana Zeka, Z. Kabir, Luke Clancy

Articles

Background: Consistent evidence has demonstrated that smoking ban policies save lives, but impacts on health inequalities are uncertain as few studies have assessed post-ban effects by socioeconomic status (SES) and findings have been inconsistent. The aim of this study was to assess the effects of the national Irish smoking ban on ischemic heart disease (IHD), stroke, and chronic obstructive pulmonary disease (COPD) mortality by discrete and composite SES indicators to determine impacts on inequalities. Methods: Census data were used to assign frequencies of structural and material SES indicators to 34 local authorities across Ireland with a 2000–2010 study period. Discrete …

The Utility Of Deformable Image Registration For Small Artery Visualisation In Contrast-Enhanced Whole Body Mr Angiography, Daniel Foley, Jacinta Browne, Xiahai Zhuang, Barry Sheane, Dearbhail O'Driscoll, Daniel Cannon, Niall Sheehy, James Meaney, Andrew Fagan

Articles

Purpose;

An investigation was carried out into the effect of three image registration techniques on the diagnostic image quality of contrast-enhanced magnetic resonance angiography (CE-MRA) images.

Methods Whole-body CE-MRA data from the lower legs of 27 patients recruited onto a study of asymptomatic atherosclerosis were processed using three deformable image registration algorithms. The resultant diagnostic image quality was evaluated qualitatively in a clinical evaluation by four expert observers, and quantitatively by measuring contrast-to-noise ratios and volumes of blood vessels, and assessing the techniques’ ability to correct for varying degrees of motion.

Results The first registration algorithm (‘AIR’) introduced significant stenosis-mimicking …

Reducing Visual Deficits Caused By Refractive Errors In School And Preschool Children: Results Of A Pilot School Program In The Andean Region Of Apurimac, Peru, Sergio Latorre-Arteaga, Diana Gil-Gonzalez, Olga Enciso, Aoife Phelan, Angel Garcia-Munoz, Johannes Kohler

Articles

Background

Refractive error is defined as the inability of the eye to bring parallel rays of light into focus on the retina, resulting in nearsightedness (myopia), farsightedness (Hyperopia) or astigmatism. Uncorrected refractive error in children is associated with increased morbidity and reduced educational opportunities. Vision screening (VS) is a method for identifying children with visual impairment or eye conditions likely to lead to visual impairment.

Objective

To analyze the utility of vision screening conducted by teachers and to contribute to a better estimation of the prevalence of childhood refractive errors in Apurimac, Peru.

Design

A pilot vision screening program in …

Economic Valuation Of The Mortality Benefits Of A Regulation On So2 In 20 European Cities, Olivier Chanel, Susann Henschel, Patrick J. Goodman, Antonis Analitis, Richard W. Atkinson, Alain Le Tertre, Ariana Zeka, Sylivia Medina

Articles

Background: Since the 1970s, legislation has led to progress in tackling several air pollutants. We quantify the annual monetary benefits resulting from reductions in mortality from the year 2000 onwards following the implementation of three European Commission regulations to reduce the sulphur content in liquid fuels for vehicles. Methods: We first compute premature deaths attributable to these implementations for 20 European cities in the Aphekom project by using a two-stage health impact assessment method. We then justify our choice to only consider mortality effects as short-term effects. We rely on European studies when selecting the central value of …

Acoustic Properties Of Concrete Panels With Crumb Rubber As A Fine Aggregate Replacement, Niall Holmes, Alex Browne, Christopher Montague

Articles

This paper presents the acoustic performance of small scale crumb rubber concrete (CRC) panels in terms of the sound absorbance and insulation at low (63, 125, 250 & 500 Hz) and high (1000, 2000, 4000 and 5000 Hz) frequencies. Acoustic tests were conducted with differing levels of fine aggregate replacement with crumb rubber (7.5 and 15%) with four different grades following freezing and heating. Analysis of the workability, compressive strength and density are also presented. The results found that CRC performed well in terms of sound absorbance particularly with higher proportions (15% here) and grades of crumb rubber. As an …

Antimicrobial Properties Of Vertically Aligned Nano-Tubular Copper, Kafil Razeeb, Joanna Podporska-Carroll, Mamun Jamal, Maksudul Hassan, Michael Nolan, Declan Mccormack, Brid Quilty, Simon Newcombe, Suresh Pillai

Articles

In this work, the antimicrobial properties of vertically aligned nano-tubular Cu arrays (NT-Cu) fabricated via a template-based electrodeposition approach were investigated. The NT-Cu display good bactericidal activity against S.aureus and bacteriostatic properties against E.coli, S.sonnei, S.enterica and C.albicans. In contrast, Cu-foil electrodeposited from the same solution shows low biological activity against the same microorganisms. The antimicrobial activity of NT-Cu depends on both the type of microorganism and exposure time. After 6 hours of exposure, over 99.99% (log red = 4.43) of S.aureus population was inactivated, whereas, for E.coli, S.sonnei, S.enterica and C.albicans it was 97.8, 94.2, 89.9, and 90.3%, respectively.

The Association Of Cold Weather And All-Cause And Cause-Specific Mortality In The Island Of Ireland Between 1984 And 2007, Ariana Zeka, Stephen Browne, Helen Mcavoy, Patrick Goodman

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Background: This study aimed to assess the relationship between cold temperature and daily mortality in the Republic of Ireland (ROI) and Northern Ireland (NI), and to explore any differences in the population responses between the two jurisdictions. Methods: A time-stratified case-crossover approach was used to examine this relationship in two adult national populations, between 1984 and 2007. Daily mortality risk was examined in association with exposure to daily maximum temperatures on the same day and up to 6 weeks preceding death, during the winter (December-February) and an extended cold period (October-March), using distributed lag models. Model stratification by age and …

Offering Minority Communities Equal Opportunities Through Entrepreneurship, Thomas M. Cooney

Articles

A substantial body of research has been undertaken in recent years giving prominence to the additional and distinctive challenges faced by female entrepreneurs and by ethnic entrepreneurs. However, other groups of minority entrepreneurs have received relatively little attention and so remain underexplored within entrepreneurship literature. This article introduces some of these minority communities (Ex–Prisoners, Disabled People, Travellers/Gypsies, Grey and Gay) and highlights the research opportunities that exist for entrepreneurship academics who might wish to analyse such ‘silent’ minorities. These communities are all relatively large in terms of population numbers, require tailored support to overcome distinctive economic, social and personal obstacles, …

Breast Cancer Detection Using Interferometric Music: Experimental And Numerical Assessment, Giuseppe Ruvio, Raffaele Solimene, Antonio Cuccaro, Domenico Gaetano, Jacinta Browne, Max Ammann

Articles

Purpose: In microwave breast cancer detection it is often beneficial to arrange sensors in close proximity to the breast. The resulting coupling generally changes the antenna response. As an a priori characterization of the radio-frequency system becomes difficult, this can lead to severe degradation of the detection efficacy. The purpose of this paper is to demonstrate advantages of adopting an Interferometric Multiple Signal Classification (I-MUSIC) approach due to its limited dependence 25 from a priori information on the antenna. The performance of I-MUSIC detection was measured in terms of Signal-to-Clutter Ratio (SCR), Signal-to-Mean Ratio (SMR) and Spatial Displacement (SD) and …

Apple Pomace As A Potential Ingredient For The Development Of New Functional Foods, Sofia Reis, Dilip K. Rai, Nissreen Abu-Ghannam

Articles

Extruded snacks and baked scones were formulated with increasing levels (0-30%) of apple pomace (AP). The incorporation of up to 20% of AP in extruded snacks and in baked scones does not change significantly (pβ-carotene/linoleic acid system) increased when compared to the products to which no AP was added. Chlorogenic acid and quercetin were the major phenolic compounds found in the products. Loss of phenolic compounds during heat treatment occurred, however the DPPH radical scavenging activity of final products was not affected.

Zein Film: Effects Of Dielectric Barrier Discharge Atmospheric Cold Plasma, Shashi Pankaj, Carmen Bueno-Ferrer, N. Misra, Paula Bourke, Patrick J. Cullen

Articles

Dielectric barrier discharge atmospheric plasma is a novel non-thermal technology for the food and packaging industry. The effects of dielectric barrier discharge plasma on the surface, structural, thermal and moisture sorption properties of edible zein films have been examined. Plasma treatment increased the surface roughness and equillibrium moisture content of the zein film in a direct relationship with the applied voltage level. No significant difference in the thermal stability of the zein film is also observed after plasma treatment. Dielectric barrier discharge plasma treatments of zein film lead to a change in the protein conformation which is confirmed by X-ray …

Dielectric Barrier Discharge Atmospheric Cold Plasma For Inactivation Of Pseudomonas Aeruginosa Biofilms, Dana Ziuzina, Sonal Patil, Patrick J. Cullen, Daniela Boehm, Paula Bourke

Articles

In recent years, atmospheric cold plasma (ACP) has been widely investigated for potential application as an alternative decontamination technology in biomedical and healthcare sectors. In this study, the antimicrobial efficacy of ACP against Pseudomonas aeruginosa biofilms was investigated. The 48-h biofilms were treated inside sealed polypropylene containers with a high-voltage dielectric barrier discharge (DBD) ACP (80 kVRMS) and subsequently stored for 24 h at room temperature. Treatment for 60 s by either the direct or indirect mode of ACP exposure (inside or outside plasma discharge, respectively) reduced bacterial populations by an average of 5.4 log cycles from an initial 6.6 …

Play Hard, Work Harder. Alternative Reality Game And Scenario Based Assessments For Learning., Barry Ryan

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In this paper, an evaluative case study is detailed as an example of alternative reality game and scenario based assessments for learning. This pedagogic approach is evaluated and recommendations for practice offered. Integrating technology into the assessment process, and final student product, influenced the chosen pedagogy. The use of technology permitted this assessment approach to be adopted for a medium sized (n=40) student cohort. The use of wikis, eportfolios and digital reflective diaries were central to creating a learning environment that centralised the student and allowed them to construct and create their knowledge through scaffolded alternative reality games and scenarios. …

Development Of A Novel Functional Soup Rich In Bioactive Sulforaphane Using Broccoli (Brassica Oleracea L. Ssp. Italica) Florets And Byproducts., Laura Alvarez Jubete, Juan Valverde, Kate Kehoe, Kim Reilly, Dilip K. Rai, Catherine Barry-Ryan

Articles

Broccoli florets are rich in health-promoting compounds such as glucoraphanin, the precursor of the bioactive compound sulforaphane. In addition, broccoli byproducts such as broccoli stalk also contain health promoting compounds and represent attractive ingredients in the development of functional foods. The bioconversion of glucosinolates into bioactive isothiocyanates is affected by many factors including heat and therefore cooking of Brassicas such as broccoli may result in significant loss of sulforaphane production. The aim of this study was to develop a suitable food system as a vehicle for the delivery of sulforaphane in the human diet in adequate quantities. To this end, …

Human Factors Engineering At Design Stage: Is There A Need For More Structured Guidelines And Standards?, Farzad Naghdali, Maria Chiara Leva, Nora Balfe, Samuel Cromie

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Human Factors Engineering (HFE) focuses on the application of human factors knowledge to the design and construction of socio-technical systems. The objective is to ensure systems are designed so as to optimise the human contribution to production and minimise potential f r design-induced risks to health, personal or process safety or environmental performance (OGP, 2011). The ISO standard ISO 9241-210 (2010), Ergonomics of human-system interaction, requires that all new facilities projects apply the principles of Human Factors Engineering (HFE) during early design stages. In practice this means ensuring, as a minimum, that every new facilities project is screened in collaboration …