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All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

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A Descriptive Study Of Range Livestock Operations In The Somali Central Rangelands, Abdinasir M. Abdulle May 1990

A Descriptive Study Of Range Livestock Operations In The Somali Central Rangelands, Abdinasir M. Abdulle

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Information on livestock operations, particularly what kind of livestock operations are practiced in the Somali central rangelands, and how these operations differ for different herders in different vegetation types and farming systems, could serve as a major tool in making management decisions. This study provides a detailed description of range livestock operations in the Somali Central Rangelands. It also answers some important management questions.

Range livestock operations were inventoried through personal interviews with the owners and herders. Data are supplemented by previous nation-wide and regional survey statistics. Herd productivity data which include herd structures, age, age-related sales, slaughters, gifts, and …


Natural-Resources Education In Utah's Public Schools, John J. Van Niel May 1990

Natural-Resources Education In Utah's Public Schools, John J. Van Niel

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Several aspects of natural-resources education were explored in this study. First, a statewide survey of science, social studies, and elementary teachers was conducted to determine the current form and extent of natural-resources education in Utah. Returns were received from 51% of the surveyed teachers. The percent of time devoted to natural resources, the factors affecting natural-resources instruction, and the need to improve that instruction were assessed.

Utilizing that information, the Natural-Resources Curriculum Framework was developed. The Framework is designed to provide educators with the essential concepts to be included in a comprehensive natural-resources program.

Finally, examples of classroom support materials …


Recreation Specialization And Preferences Of Utah Anglers, Bruce P. Andersen May 1990

Recreation Specialization And Preferences Of Utah Anglers, Bruce P. Andersen

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

The purposes of this study were to gain a better understanding of anglers and angler preferences and to examine differences between subgroups of anglers based on level of specialization. Understanding these similarities and differences between user groups will provide valuable input for more effective fisheries management.

A randomized mail survey was sent to resident, adult fishing-license holders in Utah. A 68% response rate yielded 1216 usable returns for data analysis. An analysis of three recreation specialization dimensions (participation, equipment and investment, and lifestyle) and further analysis of participation and investment variables were used to develop a typology of Utah anglers. …


Forest Canopies: Form And Functional Relationships, Steven B. Jack May 1990

Forest Canopies: Form And Functional Relationships, Steven B. Jack

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Forest canopy structure is strongly influenced by stand density due to changing competitive interactions among the individual trees and in turn directly influences stemwood volume production. The structure and dynamics of forest canopies, particularly in relation to the production of stemwood, were examined in unmanaged, even-aged stands of two dissimilar tree species, Pinus contorta var. latifolia Engelm. and Abies lasiocarpa (Hook.) Nutt. The analysis of structure-production relationships was guided by a conceptual model which generated hypotheses and led to examination of assumptions incorporated in the model.

Mean crown dimensions were related to stand density through a negative exponential function for …


The Response Of Coyotes To Novel And Familiar Visual And Olfactory Stimuli, Yiting Zhang May 1990

The Response Of Coyotes To Novel And Familiar Visual And Olfactory Stimuli, Yiting Zhang

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

The purpose of this study was to explore the response v of coyotes (Canis latrans) to certain olfactory and visual stimuli. It was assumed that the findings would be of value in refining techniques used in sampling and controlling coyotes. The specific objectives were to determine (1) if coyotes were more likely to approach and remain in the vicinity of a familiar than unfamiliar scent, (2) if the response to olfactory and visual stimuli differed, (3) if positively reinforcing an approach to the stimuli differentially altered the response to visual and olfactory stimuli, and (4) if the response …


The Effect Of Seasonal Cattle Grazing On California Bighorn Sheep Habitat Use, Melanie J. Steinkamp May 1990

The Effect Of Seasonal Cattle Grazing On California Bighorn Sheep Habitat Use, Melanie J. Steinkamp

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

The effect of seasonal cattle grazing on a newly reintroduced population of California bighorn sheep (Ovis canadensis californiana) in Big Cottonwood Canyon, Idaho, was studied. The hypothesis that bighorn sheep avoid cattle was tested. The issue of avoidance between bighorn sheep and livestock is arguable. Some studies have found that bighorn sheep avoid cattle while others have found no response of bighorn sheep to cattle.

Evidence was found to document the avoidance of cattle by bighorn sheep. The size of the bighorn's home range and core area decreased with the movement of cattle into areas of high bighorn …


The Importance Of Cover For Juvenile Rainbow Trout In Lentic Systems: Field Observations And An Experimental Study On Predation, Roger Allen Tabor May 1990

The Importance Of Cover For Juvenile Rainbow Trout In Lentic Systems: Field Observations And An Experimental Study On Predation, Roger Allen Tabor

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Juvenile rainbow trout Oncorhynchus mykiss stocked into mid-elevation reservoirs in Utah are vulnerable to predation from piscivorous fish and birds. I determined how effectively juvenile trout used cover to avoid these predators by (1) direct observations (snorkel transects) of habitat selection in two reservoirs and (2) measurements of survival and growth rates in a pond experiment where adult brown trout Salmo trutta were predators. Observations of juvenile trout were conducted within five weeks of stocking in 1988 and 1989. During the day, juvenile trout were abundant in complex inshore habitats and avoided simple habitats such as sand and gravel. Measurements …


Irrigation Management Considering Soil Variability And Climatic Uncertainty, Dani Or May 1990

Irrigation Management Considering Soil Variability And Climatic Uncertainty, Dani Or

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

The increase in multipurpose use of water resources, dwindling xii water supplies, and a decline in the economic viability of many agricultural operations make efficient irrigation management imperative. Soil variability and climatic uncertainty are major obstacles to efficient irrigation management due to a lack of reliable information for decision making. This study was aimed at identifying sources of uncertainty and examining methods for reducing the effects of uncertainty on irrigation scheduling schemes.

A method for spatial-temporal estimation that combines spatial information from few measurements with a physically based model of the system dynamics is proposed. The spatial phase of the …


Seasonal Movement Patterns Of Coyotes In The Bear River Mountains Of Idaho And Utah, Glen F. Gantz May 1990

Seasonal Movement Patterns Of Coyotes In The Bear River Mountains Of Idaho And Utah, Glen F. Gantz

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Coyotes (Canis latrans) prey upon domestic sheep. The Animal Damage Control (ADC) program currently relies heavily on aerial gunning in winter to control coyote depredations on mountain grazing allotments. Some people claim that winter aerial gunning is not effective because coyotes migrate to lower elevations during winter, following herds of big game animals, and may not be on the allotments where summer depredations occur. I studied the seasonal movement patterns of coyotes in the Bear River Range of Utah and Idaho to determine if coyotes in montane habitats move on a seasonal basis. Radio-collared coyotes were located from fixed-wing aircraft …


Purification And Immunological Reactivity Of Commercial Microbial Milk Clotting Enzyme Preparations, Chima I. Osuala May 1990

Purification And Immunological Reactivity Of Commercial Microbial Milk Clotting Enzyme Preparations, Chima I. Osuala

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Commercial microbial milk clotting enzyme preparations were purified by immunoaffinity chromatography using purified antibody covalently coupled to porous glass beads as the column matrix. Commercial enzyme preparation diluted in 1 mM sodium acetate buffer at pH 5.0 was then biospecifically adsorbed to the column matrix by end-over-end mixing of the glass-antibody complex in the enzyme solution for 12 h at 5°C. The antibody bound enzyme adsorbed glass beads were soaked in .2 M glycine or ethanolarnine at pH 7.0 to block uncoupled reactive sites on the matrix. Following this, the column was washed with 1 mM sodium acetate …


Enhancing Female Adolescent Identity Development Through Ideological Perspective-Taking Training: A Replication And Extension Study, Deborah Lynn Huston May 1990

Enhancing Female Adolescent Identity Development Through Ideological Perspective-Taking Training: A Replication And Extension Study, Deborah Lynn Huston

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

The purpose of this study was to determine the effect of ideological perspective-taking training on female identity development. The primary hypothesis of the study was that those subjects engaged in the training sessions would show advancement in identity development that exceeded that of normal adolescent development. Ninety-eight subjects were pretested for the study. Attrition due to elimination during prescreening and failure to complete the training phase of the study left 50 subjects. Participants were assigned to a treatment group, an engaged control group, or a maturational control group. Both the treatment and engaged control groups participated in two sessions a …


The Basis Of Browsing Tolerance In Shrubs Of The Intermountain West: Growth Rates And Meristematic Potential, Jackson L. Wandera May 1990

The Basis Of Browsing Tolerance In Shrubs Of The Intermountain West: Growth Rates And Meristematic Potential, Jackson L. Wandera

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Relative growth rates (RGRs) and meristematic potential of big sagebrush (Artemisia tridentata, in some experiments ssp. tridentata Nutt. and in other experiments ssp. vaseyana [Rydb] Beetls), bitterbrush (Purshia tridentata [Pursh] DC), serviceberry (Amelanchier alnifolia Nutt.), birchleaf mountain mahogany (Cercocarpus montanus Raf.), curlleaf mountain mahogany (C. ledifolius Nutt.) and big tooth maple (Acer grandidentatum Nutt.) were quantified under both glasshouse and field conditions. The relationships between the RGRs, meristematic potential and regrowth capacity of the shrub species were then determined. Sagebrush exhibited a significantly higher RGR than the other species at both high and low …


Reproductive Ecology Of Wyoming Big Sagebrush (Artemisia Tridentata Ssp. Wyomingensis) : Effects Of Herbivory And Competition, Richard T. Decker May 1990

Reproductive Ecology Of Wyoming Big Sagebrush (Artemisia Tridentata Ssp. Wyomingensis) : Effects Of Herbivory And Competition, Richard T. Decker

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Herbivory and plant competition affect sexual reproduction of plants in various ways. Exclusion of mule deer (Odocoileus hemionus) and cattle, removal of plant competition (both inter- and intraspecific), and all combinations of the above treatments were used to examine the individual and combined affects on Artemisia tridentata ssp. wyomingensis (Wyoming big sagebrush) reproduction. Reproduction of Artemisia tridentata ssp. wyomingensis was divided into hierarchical levels of the number of: (1) modules per current-year ' s growth (CYG), (2) nodes per module, (3) inflorescence heads per node, (4) achenes per inflorescence head and (5) percent viable achenes. Counts at hierarchical …


Socio-Behavioral Correlates Of 6- To 11-Year-Old Offspring Of Alcohol Consuming Parents, Jan Garver Bacon May 1989

Socio-Behavioral Correlates Of 6- To 11-Year-Old Offspring Of Alcohol Consuming Parents, Jan Garver Bacon

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

There is a lack of simple random sample based research into whether there are social skill and behavior problem differences for six- to eleven-year-old boys and girls which correlate with rates of parental alcohol consumption, social sequelae of parental alcohol consumption, reported level of marital conflict, and extended family history of alcoholism.

This simple random sample study correlates the above variables with T scores on the behavior problem and social competence scales of the Achenbach Child Behavior Checklist and Child Behavior Checklist - Teacher's Report Form for six- to eleven-year-old children (N=100). Behavior problem scales include disorders of affect, thought, …


Public School Preschool For Disadvantaged Children: Program Quality And Long-Term Effects, Ellen Frede May 1989

Public School Preschool For Disadvantaged Children: Program Quality And Long-Term Effects, Ellen Frede

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Although preschool for disadvantaged children is strongly supported nationally as a beneficial endeavor, the research that underlies this support has mostly been limited to small-scale, high-quality experiments. Some have questioned the ability of public schools to provide similar experiences for young children and to produce long-term effects. This study is a long-term follow-up in first-grade children who attended preschool in a statewide public school program. The classrooms were observed to assess the quality of treatment. It was determined that children who attended classrooms that provided medium- to high-quality experiences outperformed those in poor-quality classrooms, as well as comparison children who …


The Involvement And Relationship Of Ferritin And Ceruloplasmin In Oxygen Radical Toxicities, David Wilson Reif May 1989

The Involvement And Relationship Of Ferritin And Ceruloplasmin In Oxygen Radical Toxicities, David Wilson Reif

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

The intent of this dissertation is to demonstrate that ferritin iron may be involved in alloxan, diquat, and radiation-dependent damage and that ceruloplasmin may afford protection by limiting the availability of ferritin iron. Initial studies demonstrated that the alloxan radical formed by glutathione-dependent reduction of alloxan reductively mobilized ferritin iron and the released iron catalyzed lipid peroxidation. SOD and catalase were without effect, whereas ceruloplasmin effectively inhibited both iron release and lipid peroxidation. Using 137Cs gamma-radiation it was demonstrated that radiolytically generated superoxide released iron from ferritin with an efficiency of 67%. Lipid peroxidation strictly required both superoxide and …


Peptide Mapping Analysis Of Vp5 And Vp7 From Five Bluetongue Viruses Isolated In The United States, Yi-Yuan Yang May 1989

Peptide Mapping Analysis Of Vp5 And Vp7 From Five Bluetongue Viruses Isolated In The United States, Yi-Yuan Yang

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

BTV-2, 10, 11, 13 and 17 were purified by three discontinuous sucrose gradients. Viral proteins were separated from the genomic double-stranded RNA using a newly developed KCI-SDS (sodium dodecyl sulfate) precipitation method. Cyanogen bromide (CNBr) peptide mapping of VP5 and VP7 from bluetongue virus (BTV) serotypes 2, 10, 11, 13 and 17 was then done using silver-stained SDS-polyacrylamide gels. The antigenic epitopes of VP5 and VP7 digested with CNBr were also investigated with polyclonal antibodies produced against KCl-SCS precipitated BTV-13 viral proteins. The peptide maps and the antigenic epitopes of VP7 were similar among the five American serotypes with very …


Purification And Characterization Of A Chemically Induced Epstein-Barr Virus-Associated Deoxyribonuclease, Guang-Yuh Hwang May 1989

Purification And Characterization Of A Chemically Induced Epstein-Barr Virus-Associated Deoxyribonuclease, Guang-Yuh Hwang

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Purification of Epstein-Barr virus-associated deoxyribonuclease (EBV-DNase) from Raji and P3HR-1 cells treated with 12-0-tetradecanoylphorbol-13-acetate and sodium butyrate was performed by sequential ion-exchange column chromatography and fast protein liquid chromatography. The enzyme activity, protein concentration, yield, specific activity, purification profiles, and polypeptide patterns by electrophoretic analysis in each column purification step were determined. The characteristics of the partially isolated EBV-DNase were demonstrated by the enzyme activity, DNA binding affinity, and inhibition by the nasopharyngeal carcinoma patient sera and rabbit polyclonal antibodies against the partially purified EBV-DNase. A nonisotopic assay was developed as a new method in detecting the nuclease. …


Obesity Management: A Meta-Analysis Of Key Factors, David E. Christian May 1989

Obesity Management: A Meta-Analysis Of Key Factors, David E. Christian

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Obesity is a prevalent and refractory disorder. This study consisted of a meta-analysis of research focusing on diet and exercise treatments and their impact on obesity related measures. Only studies that report data for periods of 6 months or more after treatment onset were included. Forty-four studies were analyzed. These were drawn from 16 previous reviews of the literature as well as several computer data bases.

Following are some of the tentative conclusions drawn: (a) diets consisting of improved nutritional quality yield superior results to restricted calorie diets; (b) flexible, self-directed exercise yields better results than more regimented exercise; (c) …


Modeling Forest Dynamics Based On Stand Level Resource Allocation, Geoffrey Candler Poole May 1989

Modeling Forest Dynamics Based On Stand Level Resource Allocation, Geoffrey Candler Poole

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

An ecologically based model of forest succession is presented. In the model, trees compete for a share of limited growth resources available from their environment. Competition is reflected by each tree's effect on the resource pool and is not explicitly modeled. Model parameters were fit to field data from subalpine forests of the Rocky Mountains. A technique for estimating model parameters from understory-tolerance rankings and silvical characteristics of each species is also presented. The model's output was consistent with our current understanding of forest dynamics. Emergent properties of the model also mimicked natural processes such as self-thinning, release, and maximum …


Using Computer Imaging To Assess Visual Impacts Of Forest Insect And Disease Pests, Daniel Rabin May 1989

Using Computer Imaging To Assess Visual Impacts Of Forest Insect And Disease Pests, Daniel Rabin

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Forest insect and disease pests alter the appearance of trees, thereby impacting visual resources. Because of the complexity of most forest landscapes, the degree of visual impact of pest-infested forest stands is difficult to quantify.

This paper describes a method of measuring visual impacts of pest-infested forest stands. Photographs of healthy Ponderosa pine trees were entered into a computer video-image-processing system. Using this system, images of trees were altered to simulate different degrees of infestation by limb rust, a forest pathogen.

The altered and unaltered images were shown to groups of observers who rated the scenes in terms of "scenic …


Seasonal Variation In The Species Composition, Abundance, And Size-Frequency Distribution Of Zooplankton In Bear Lake, Utah-Idaho, Edmundo G. Moreno May 1989

Seasonal Variation In The Species Composition, Abundance, And Size-Frequency Distribution Of Zooplankton In Bear Lake, Utah-Idaho, Edmundo G. Moreno

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Bear Lake, a large oligotrophic lake (282 km2), was studied from October 1986 to December 1987 to determine the temporal changes in the zooplankton assemblage at one site in the pelagic zone and one in the littoral area. In this study, species composition, abundance, biomass, and size frequency distribution were determined. Additionally, chlorophyll a, water transparency, and temperature and oxygen profiles were measured to help interpret zooplankton changes during the study.

The zooplankton species assemblage comprised eight species of cladocera, seven species of copepods, and five species of rotifers. The abundance and biomass of the zooplankton assemblage, particularly …


A Preschool-Age Neurodevelopmental Comparison Between Normal-Birthweight Infants And Low-Birthweight Infants With And Without Intraventricular Hemorrhage, William Frederick Corey May 1989

A Preschool-Age Neurodevelopmental Comparison Between Normal-Birthweight Infants And Low-Birthweight Infants With And Without Intraventricular Hemorrhage, William Frederick Corey

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Advances in medical technology have provided the mechanisms for sustaining life in premature and low-birthweight infants, resulting in the survival of more of these infants. Low-birthweight (LBW) and preterm infants are placed at risk by a number of medical complications, including intraventricular hemorrhage (IVH).

The outcome of low-birthweight infants with intraventricular hemorrhage has been the subject of a great deal of research and continues to be a much-discussed topic in the medical and psychological communities. As more data become available, it appears that more questions arise concerning the later neuodevelopmental and neuropsychological outcome of these infants.

For this reason, research …


Mate Selection In Contemporary America: An Exchange Theory Perspective, Margaret H. Young May 1989

Mate Selection In Contemporary America: An Exchange Theory Perspective, Margaret H. Young

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

The use of exchange theory as it applies to human relations has escalated dramatically in the past 20 years. The present study applies exchange theory as the basis of mate selection in contemporary society. Whereas an actual barter system was used in the past and families played a major role in choosing prospective mates, participants in the mate selection process are not virtually on their own and must rely upon their own bargaining skills to present their assets on the marriage market. A number of characteristics are thought to enhance or detract from a person's "worth" on the marriage market. …


Psychosis In A Developmental Psychopathology Context: A Factor Analytic Study Of Schizophrenia In Adolescent Psychiatric Inpatients, Paul R. Adams May 1989

Psychosis In A Developmental Psychopathology Context: A Factor Analytic Study Of Schizophrenia In Adolescent Psychiatric Inpatients, Paul R. Adams

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Demographic, historical, psychometric, and clinical data were obtained from the psychiatric files of all patients manifesting schizophrenic symptomatology who were hospitalized in an adolescent psychiatric facility during a five year period (N= 71). Factor analysis of the usable data resulted in three interpretable factors, which included: (1) aggressive behavior; (2) disturbed family functioning; and, (3) thought disorder. Age of first hospitalization correlated positively with factor three.

The results provide support for concerns expressed by a number of scientists and clinicians that schizophrenia may not be a discrete, unitary disorder; and that uncritical downward extension of adult diagnoses to adolescents and …


Neuroanatomical Asymmetry, Handedness, And Family History Of Handedness: A Study Of The Markers Of Structural And Functional Lateralization, Steven A. Lifson May 1989

Neuroanatomical Asymmetry, Handedness, And Family History Of Handedness: A Study Of The Markers Of Structural And Functional Lateralization, Steven A. Lifson

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

This study investigated the associations between (1) handedness (demonstrated preference of one hand for the performance of most unimanual tasks) and neuroanatomical asymmetry (measurable differences in width between the cerebral hemispheres) and (2) familial history of handedness (the presence of a left-handed sibling or parent of a right-handed subject) as an intervening factor in the relation between handedness and neuroanatomical asymmetry. Width measurements of the brain were derived from computerized tomographic (CT) films and grouped in to categories by hand preference (measured by the Edinburgh Handedness Inventory) and family history. The measurements of right (n=68), right with left-handed relatives (n=24), …


The Development Of The Stress-Response Scale For Adolescents, Steven Curtis May 1989

The Development Of The Stress-Response Scale For Adolescents, Steven Curtis

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Adolescence is an important period in the life cycle for which to study stress, due to the many involved developmental changes that require adaptation. This adaptation can be very stressful and result in pathology. Stress is defined as a "process" involving a continual transaction between stressors in the environment, mediating variables, and stress responses.

The Stress-Response Scale for Adolescents (SRSA) was developed to measure self-perceived stress responses of those between the ages of 14 to 20. The SRSA's development involved three studies. Study 1 involved item selection, scale construction, item reduction, and estimations of internal consistency and validity. Truthfulness items …


An Examination Of Open- And Closed-Economic Conditions In Operant Research, Craig R. Loftin May 1989

An Examination Of Open- And Closed-Economic Conditions In Operant Research, Craig R. Loftin

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

The effect of economic condition on the relation between responding and overall rate of reinforcement has been an area of recent interest in operant research. The present research was conducted to determine whether the manipulation of the economic condition, by the systematic manipulation of the provision of substitute food, has an effect on this relation and whether open- and closed-economies represent two opposing alternatives or two parametric extremes along a continuum. The results of two experiments conducted with pigeons using variable-interval and fixed-ratio schedules of reinforcement suggest that the manipulation of economic condition has a controlling effect on the relation …


Reaction Time In Elderly Subjects: The Effects Of Practice On Two Different Reaction Time Tasks, Dawn Marie Birk May 1989

Reaction Time In Elderly Subjects: The Effects Of Practice On Two Different Reaction Time Tasks, Dawn Marie Birk

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

The reaction time of four groups of elderly human subjects were examined to determine the effects of stimulus presentation and task practice. Each group practiced different tasks, each requiring a response when more than one alternative was available. Two tasks involved making responses based on either visually or auditorily presented stimuli only. One task required decisions to be made on the basis of both auditory and visual stimuli. The fourth group acted as a comparison group and did not practice a reaction-time task; although they did perform a task on the computer and their reaction times were measured. Before and …


Characterization Of Anti-Pichinde Virus Monoclonal Antibodies For The Directed Delivery Of Antiviral Drugs And Toxins, Noah Jefferson Burns Iii May 1989

Characterization Of Anti-Pichinde Virus Monoclonal Antibodies For The Directed Delivery Of Antiviral Drugs And Toxins, Noah Jefferson Burns Iii

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Mouse monoclonal antibodies directed against Pichinde virus (PCV) were produced to evaluate their application as vehicles for the delivery of antiviral drugs or toxins to virus-infected cells. Four monoclonal antibodies, PC4.9A6, PC4.9D3, PC4.7C2, and PC4.8D3, were of the IgG2a subisotype and reacted with acetone-fixed and live PCV-infected Vero-76 cells. In vivo stained splenic macrophages derived from PCV-infected hamsters that had been injected with fluorescein-labeled PC4.9A6 (FITC9A6) demonstrated a 400% increase in total fluorescence over similarly treated, non-infected cells when analyzed by flow cytometry. This is an indication that FITC-9A6 does have some ability to specifically target PCV infected cells in …