Digital Commons Network^™

Attachment Issues: Microbes, Minerals, And The Persistence Of Soil Organic Matter, Md Shafiul Islam Rion

Graduate Theses, Dissertations, and Problem Reports (ETD)

The remnants of microorganisms are now understood to account for the majority of organic matter in many mineral soils. Despite the significance of this microbial necromass for soil carbon storage, we know relatively little about how the traits of microorganisms interact with soil minerals to determine the stability of microbe derived carbon in soil. Soil minerals differ in their surface area and chemistry potentially influencing microbial attachment, biofilm formation, and the persistence of microbial necromass. To address this knowledge gap, we grew twelve bacterial species from four broad groups of varying cell wall morphology (Gram positive, Gram negative, filamentous actinobacteria, …

Specialized Metabolism In Retina, Retinal Pigmented Epithelium, And Testis, Siyan Zhu

Graduate Theses, Dissertations, and Problem Reports (ETD)

The retina and its neighboring retinal pigmented epithelium (RPE) are high energy-demanding and metabolically active tissues with specialized and complementary metabolism. They are metabolically interdependent and impact each other’s viability. Interestingly, many of the metabolic features in the retina and RPE are shared with the testis. For example, testis is also energy costly due to continuous sperm differentiation and it has similar metabolic inter-dependence between different testis cells. Both the retina and testis are vulnerable to mitochondrial metabolic impairments.

We conducted three research projects to understand 1) the nutrient utilization and communication in retina and RPE; 2) The profiling of …

Comparison Of Skeletal Class Iii Malocclusion Facial-Shape Subtypes Between Caucasian, African American, And Hispanic Populations, Rachel Katherine Huffman

Graduate Theses, Dissertations, and Problem Reports (ETD)

Abstract

Comparison of Skeletal Class III Malocclusion Facial-Shape Subtypes Between

Caucasian, African American, and Hispanic Populations

Rachel K. Huffman, DMD

Introduction: The development of a skeletal Class III malocclusion (Cl III) is complex as it can arise from the overgrowth of the mandible, undergrowth of the maxilla, or a combination of the two. To understand the heterogeneity of this trait clinically, studies have been conducted using facial shape analysis (e.g., Geometric morphometrics (GeoMorph)) of Caucasian and Asian populations to learn more about the principal shapes. Little is known, however, about the predominant principal shape components of Cl III within African …

Elucidation Of The Role Of Agouti-Signaling Protein Throughout Folliculogenesis And Early Embryonic Development In Cattle, Heather L. Chaney

Graduate Theses, Dissertations, and Problem Reports (ETD)

The oocyte expresses certain genes during folliculogenesis to regulate the acquisition of oocyte competence. Oocyte competence, which refers to the presence of imperative molecular factors in the oocyte that are critical for high oocyte quality, is directly related to the ability of the oocyte to result in a successful pregnancy following fertilization. Over the past few decades, the development and optimization of assisted reproductive technologies, particularly in vitrofertilization, have enabled the beef and dairy industries to advance cattle genetics and productivity. However, only approximately 40% of bovine embryos will develop to the blastocyst stage in vitro. In addition, bovine embryos …

Krill Protein Fortified Extrudates: A Pilot Study, Avery S. Fleeharty

Graduate Theses, Dissertations, and Problem Reports (ETD)

Antarctic krill (Euphausiacea superba) is an abundant and sustainable but underutilized natural resource with well-studied protein isolation methodology using Isoelectric solubilization-precipitation (ISP). The resulting krill protein isolate (KPI) has high yield and sufficient impurity removal in addition to desirable nutritional and functional properties but has yet to be used in food systems. Extrusion is a versatile and scalable food processing method with many benefits; thus, the aim of this study was to determine the impact of KPI as an additive in extrusion processing with the eventual goal of introducing it to human and animal food systems. KPI was …

Physical Activity Promotion, Assessment, And Engagement In Clinical Settings In The United States, Kristin A. Grogg

Graduate Theses, Dissertations, and Problem Reports (ETD)

Physical inactivity is an important contributor to morbidity and all-cause mortality and the 2018 Physical Activity Guidelines Advisory Committee recommended that physicians increase their role in physical activity assessment and promotion to combat physical inactivity and related comorbidities. Healthcare providers are increasingly called upon to initiate physical activity promotion with their patients to manage conditions like obesity, diabetes, and cardiovascular diseases. Still, recent reports indicate that less than half of primary care visits include some type of physical activity promotion. Although the National Physical Activity Plan includes some recommendations for clinicians and the healthcare sector on physical activity promotion, it …

Associations Among Executive Functioning, Family Functioning, Adolescent Responsibility, And Adherence In Pediatric Inflammatory Bowel Disease, Cecily Newel Conour

Graduate Theses, Dissertations, and Problem Reports (ETD)

Background: Inflammatory Bowel Disease (IBD) is a common cause of chronic pain for adolescents in the United States. Adherence to the treatment regimen is a significant concern, particularly for adolescents. Barriers to adherence are varied, but include cognitive factors, such as forgetting. Parent involvement is associated with increased adherence in this population, though adolescent involvement is less studied. Family functioning is associated with adherence to medication regimen across pediatric chronic illnesses, including IBD treatment regimen. To better inform clinical care, this study aims to understand the relations among adolescent’s responsibility, executive functioning, family functioning, and adherence. Methods: The current study …

Influence Of Maternal Nano-Titanium Dioxide Inhalation Exposure On Cyclooxygenase Metabolites: Maternal Microvascular And Sexually Dimorphic Feto-Placental Outcomes., Julie Anne Griffith

Graduate Theses, Dissertations, and Problem Reports (ETD)

Nano-titanium dioxide (nano-TiO₂) is an engineered nanomaterial (ENM), which is widely utilized in diverse products like surface coatings, cosmetics, medical devices, and filters. Inhaled nano-TiO₂ has been demonstrated to induce pulmonary inflammation, impair microvascular function, and hinder generational reproductive endpoints. These outcomes indicate why it is important to explore gestation, as it requires timely vascular adaptations to support maternal and fetal health and development. Therefore, the central hypothesis of my dissertation is: maternal nano-TiO₂ inhalation exposure will impact maternal microvascular function and fetoplacental hemodynamics and function in a sexually dimorphic manner.

The first purpose of …

The Role Of Digital Spaces In Caring For Children With Feeding Tubes: Home, Family, And Community Reconsidered, Sara Gilbert Loftus

Graduate Theses, Dissertations, and Problem Reports (ETD)

Abstract

This dissertation draws from feminist geographic research to critically examine the landscapes of care traversed by family caregivers of children with feeding tubes and complex medical and long-term care needs. Drawing from theories and methodologies from feminist disability and digital geographies, in combination with feminist science technology studies (STS), I explore the impact of neoliberal policy changes on daily caregiving tasks and how families, in response to these policy changes, (re)create digital spaces and relationships to satisfy their unmet needs. This research examines the growing and shifting roles of digital spaces in the everyday lives of marginalized and/or vulnerable …

Impacts Of Temperature And Salt Concentrations For Thermal Inactivation Of Salmonella In Moisture Enhanced Reconstructed Chicken Patties, Alik D. Browning

Graduate Theses, Dissertations, and Problem Reports (ETD)

The purpose of this experiment is to determine the thermal kinetic parameters of Salmonella in moisture-enhanced, reconstructed ground chicken patties as affected by temperatures and salt concentrations. Salmonella is responsible for 35% of the foodborne illnesses associated with poultry products (Batz et al., 2011). Nonintact reconstructed chicken meat is mixed with brine solutions containing various salt and polyphosphate concentrations to increase water-holding capacity, decrease cooking losses, improve sensory tasting scores, and maintain the good quality of completed chicken products (Gill et al., 2004). Increasing salt concentrations within meats can increase the thermal inactivation of pathogens. In this study, Salmonella Typhimurium …

Health Behaviors As Mediators Of Caregiver Physical Health, Michaela Clark

Graduate Theses, Dissertations, and Problem Reports (ETD)

Informal caregivers provide the bulk of care to older adults in the United States (Ali et al., 2021). Informal caregivers include family, friends, and neighbors, many of whom do not have any formal training for this role. There are costs associated with taking on a caregiving responsibility, such as scheduling appointments, helping with medications, household tasks, and even financial support (Rha et al., 2015). Many of these informal caregivers lack the coping skills necessary to manage the challenges associated with caregiving (Xu et al., 2021). Many also take on this role despite the personal costs on one’s own health, time, …

Social Determinants Of Health And Functional Disability At Mid- And Late-Life, Carly Pullen

Graduate Theses, Dissertations, and Problem Reports (ETD)

The number and proportion of older adults living in the U.S. and globally are increasing. Not only are more adults living longer (Rania et al., 2021), there is evidence that people are

entering late life with more functional disability than in previous generations (He & Larsen,

2014). Social determinants of health include demographic and environmental influences than can impact a range of health outcomes beyond more direct risk factors (Duran and Perez-Stable, 2019). Using data from 243,693 community-dwelling adults who completed the 2020 Behavioral Risk Factor Surveillance System survey, we tested the associations among social determinants of health (i.e., age, …

Rumen And Urine Amine/Phenol-Metabolome Of Beef Steers With Divergent Residual Feed Intake, Taylor S. Sidney

Graduate Theses, Dissertations, and Problem Reports (ETD)

Cattle production is one of the most important agricultural industries in the United States. Despite its overall production efficiency, the industry is facing considerable adversities to its long-term sustainability due to population growth, decreased land availability, and the increase in global warming. Since feed efficiency imposes an immense influence on the cost of production for animals and is a major cost determinant for profitability, the improvement of feed utilization through the understanding of animal-to-animal variation in feed efficiency is pertinent to increase farm productivity and profitability. Residual feed intake (RFI) is a measure of feed efficiency and is defined as …

Gut-Lung Axis Of The Microbiome Alterations After Ultrafine Particles And Ozone Inhalation, Md Habibul Hasan Mazumder

Graduate Theses, Dissertations, and Problem Reports (ETD)

Air pollution poses a significant threat to public health. It is a mixture of particulate matter, gaseous substances, and other secondary substances. Despite epidemiological research indicating the interactive effects of various components of air pollution, existing studies and regulatory limits often focus on single-exposure scenarios. The emerging understanding of the microbiome’s role in regulating immune response, metabolism, and pathophysiology of various diseases points towards interactions between environmental exposures and microbial dysbiosis. In this dissertation, mice were exposed to relevant deposited doses of carbon black (CB) and ozone (O3), and lung inflammation as well as the lung-gut axis of the microbiome …

Longitudinal Sport Science Implementation In American Collegiate Men’S Basketball, Jason Stone

Graduate Theses, Dissertations, and Problem Reports (ETD)

The expanding opportunities to implement sport science frameworks in elite-level basketball environments coincide with the sport’s increasing global prominence. Concomitant to these opportunities is the continual growth of the sport technology market (e.g., wearables, force plates) and computational power (e.g., data management tools, coding capabilities), which yields solutions and challenges for both athletes and practitioners. Due to the rapid influx of new sport technologies in high performance environments, particularly American Collegiate Men’s Basketball, more formal and ecologically valid research on how to effectively utilize data derived from them, particularly over long periods of time (i.e., multiple seasons) is needed. To …

Cell Signaling And Stress Response In The Yeast Saccharomyces Cerevisiae: A Study Of Snf1, Scott E. Arbet Ii

Graduate Theses, Dissertations, and Problem Reports (ETD)

Saccharomyces cerevisiae are yeast that are unicellular eukaryotic organisms that are well studied as a model organism for understanding fundamental cellular processes. The ability of yeast to sense nutrient availability is crucial for their survival, growth, and reproduction. Yeast cells use various mechanisms to sense and respond to nutrient availability, including transporter-mediated uptake, receptor-mediated signaling, and sensing of metabolites. The subcellular localization of nutrient-sensing components is crucial for yeast function in nutrient sensing and signaling. Protein complexes, such as the AMP-activated protein kinase (AMPK) pathway, in nutrient sensing and response, as well as the downstream effects of these pathways …

The Investigation Of Novel Bovine Oocyte-Specific Long Non-Coding Rnas And Their Roles In Oocyte Maturation And Early Embryonic Development, Jaelyn Zoe Current

Graduate Theses, Dissertations, and Problem Reports (ETD)

Early embryonic loss is a significant factor in livestock species' infertility, resulting in an economic deficit. In cattle, the in vivo fertilization rate is ~90%, with an average calving rate of about 55%, indicating an embryonic-fetal mortality rate of roughly 35%. Further, 70-80% of total embryonic loss in cattle occurs during the first three weeks after insemination, particularly between days 7-16. Growing evidence indicates that the oocyte plays an active role in regulating critical aspects of the reproductive process required for successful fertilization, embryo development, and pregnancy. However, defining oocyte quality remains enigmatic. Recently, many have abandoned the notion that …

Sexual Misconduct Against Female Athletes: Knowledge, Training, And Readiness To Act Of Future Sport Leaders, Mackenzie B. Luikart

Graduate Theses, Dissertations, and Problem Reports (ETD)

The purpose of this study is to understand the comprehensiveness and effectiveness of current education in sport related degree programs and the impact that has on future and current sport leaders to confront and report sexual misconduct in sport.

A total of 15 participants were selected to participate in this study. The participants for this study include current graduate students pursuing degree programs in either coaching studies or sport management, and graduates of these two programs (within the last five years) who are currently in the workforce in their respective fields. Most participants were white, female, and currently served a …

Evaluation Of The Dynamic Indicators Of Basic Early Literacy Skills (Dibels 8th Edition) Measures For Use With The Energy Express Summer Literacy Program, Maria Boki

Graduate Theses, Dissertations, and Problem Reports (ETD)

The present study examines the suitability of the DIBELS 8^th edition assessment for use within the scope and context of the Energy Express summer literacy program. The 6-week summer program helps children in grades K-5 from rural and low-income West Virginia communities improve or maintain their reading levels over the summer months. Standard, grade-appropriate DIBELS 8^th subtests were administered to 1,065 participants at the beginning and end of Summer 2022. The resulting pre- and post-scores were cross-referenced with demographic data and attendance rates from 20 counties and 35 sites in West Virginia. They were then analyzed for correlations …

Survival Of Listeria Monocytogenes On Organic Honeycrisp And Fuji Apples During Storage At 5, 12 And 22.5◦C, Connor S. Freed

Graduate Theses, Dissertations, and Problem Reports (ETD)

This study aimed to evaluate and model the survival of Listeria monocytogenes on organic HoneyCrisp (HC) and Fuji (FJ) apples during storage at various temperatures. Fresh organic HC and FJ apples (without wax coating) obtained from a local wholesale market were inoculated with a 2-strain mix of L. monocytogenes followed by storage at 5.0 (22.9% RH), 12.0 (37.0% RH), and 22.5oC (50.4% RH) for 60, 35, 7 days, respectively, and periodically (day-0 to 60) analyzing microbial populations. Surviving L. monocytogenes were spread-plated on Modified-Oxford agar after 10 or 100-fold serial dilution. Data were analyzed using the mixed-model procedure of SAS …

Clinical And Economic Burden Of Inflammatory Chronic Conditions Among Adults In The United States: Evidence From Three Nationally Representative Surveys, Mohammad Ikram

Graduate Theses, Dissertations, and Problem Reports (ETD)

A significant proportion of adults in the United States (US) suffer from various inflammatory chronic conditions (ICCs). They include a wide range of diseases such as diabetes, rheumatoid arthritis, asthma, and chronic obstructive pulmonary diseases. ICCs are not curable, and their management typically involves medications such as NSAIDs and corticosteroids. Recently, a whole health approach that places patients’ well-being at the center is increasingly being used for better management of ICCs. The whole health approach uses an integrated approach that includes multimodal management with complementary and alternative medicines (CAM) and prescription drugs. However, the use of CAM and prescription drugs …

Lipid Binding Properties Of Huntingtin As A Novel Therapeutic Target, Chathuranga Siriwardhana

Graduate Theses, Dissertations, and Problem Reports (ETD)

As protein aggregation is the defining hallmark of all amyloid diseases, a common therapeutic strategy is to develop molecules that inhibit aggregation. However, this approach has yielded limited success. Many amyloid proteins directly interact with lipid membranes. These interactions promote distinct aggregation pathways and often result in membrane damage leading to toxicity. As a result, directly targeting the ability of amyloids to bind lipid membranes represents a novel therapeutic strategy. As a proof of principle, the interaction between lipid membranes and mutant huntingtin protein (htt) aggregates was used to test this strategy. Mutant htt containing an expanded polygulatmine (polyQ) domain …

Effects Of Development On Hpa Function Following Pubertal Stress, Brittany D. Elliott

Graduate Theses, Dissertations, and Problem Reports (ETD)

For women, two of the greatest risk factors for affective disorders are adversity experienced during puberty and later becoming pregnant. We have created a translationally relevant mouse model where we address these complex risk factors. Previously, we discovered that pregnant mice (dams) that experienced chronic variable stress (CVS) during puberty display a blunted hypothalamic-pituitary-adrenal (HPA) response when exposed to an acute stressor. Interestingly, this alteration only first becomes apparent during pregnancy, which is a sensitive period for these effects due to normative neuroendocrine changes. Further investigation of the mechanisms underlying this dysfunction revealed altered gene expression in the paraventricular nucleus …

The Expectations, Experiences And Satisfaction Of Students Within A Nutrition And Dietetics Program Regarding Faculty Academic Advising Using A Prescriptive Or Developmental Advising Lens, Nettie Puglisi Freshour

Graduate Theses, Dissertations, and Problem Reports (ETD)

This research study examined student’s expectation, experiences and satisfaction with faculty academic advising using a prescriptive vs. developmental lens. The intent was to explore the student’s expectations of academic advising, determine if their experiences aligned with their expectation and if this led to satisfaction of their academic advising. This study utilized the Systems theory to determine how academic advising is performed by the program and if that experience for the student is how the systems (institution, college, department, and program) are promoting the form of advising. This study utilized a qualitative case study approach, guided by three research questions. Data …

Ambient Ammonia Synthesis Via Microwave-Catalytic Materials And Plasma Chemistry, Siobhan Brown

Graduate Theses, Dissertations, and Problem Reports (ETD)

Ammonia is critical to supporting human life on earth because of its use as fertilizer. The Haber-Bosch process to produce ammonia has been practiced for over 100 years. This process operates at high pressure and temperature to overcome the thermodynamic and kinetic limitations of the ammonia synthesis reaction thus researchers have tried to overcome it for decades. At present this process represents 1% of global energy usage and 2.5% of global CO₂ emissions. The proposed chemical looping ammonia synthesis approach seeks to reduce the environmental impact of this critical process and to elucidate microwave-catalytic principles.

This research aims to …

Exploring The Biological Basis Of Residual Feed Intake In Beef Cattle Using Multi-Omics Analysis., Godstime Ayobami Taiwo

Graduate Theses, Dissertations, and Problem Reports (ETD)

Efficient feed utilization is critical for both economic sustainability and environmental responsibility in modern livestock production. While there has been extensive research, the multifaceted nature of feed efficiency remains complex, with many biological factors still unexplored. This dissertation examines the physiological foundations of feed efficiency by elucidating some of the complex biological mechanisms associated with residual feed intake (RFI) phenotype in beef cattle, using a range of Omics approaches. We hypothesized that metabolites related to amino acids, carbohydrates, and fatty acids could act as potential biomarkers for RFI. Through a chemical group-based metabolomics method, we identified enriched pathways in feed-efficient …

Descriptions Of Breast Self-Care From Inner-City African American And Hispanic Women Seeking Care In A Comprehensive Breast Care Center, Salamata Yoda

Graduate Theses, Dissertations, and Problem Reports (ETD)

Background: A plethora of inquiries has irrefutably revealed a decline in the death rates of breast cancer in some ethnic populations due to timely diagnoses, and enhancement in treatment measures. However, not all women have gained equal access to these innovations in healthcare. Certainly, as a repercussion of the noted disparity, the overall breast cancer prevalence among African American and Hispanic women has steadily escalated. Although no single test can detect all breast cancers early, practicing breast self-care consisting of breast self-awareness (BSA) in combination with other screening methods such as clinical breast exam (CBE), mammogram screening, and a suitable …

Nature Based Interventions For Human Health And Wellbeing, Samantha I. Moyers

Graduate Theses, Dissertations, and Problem Reports (ETD)

Background. Nature exposure is beneficial to human health and wellbeing, but the evidence base for nature-based interventions (NBI) is still developing. Heterogeneous naming conventions, study designs, and intervention contexts create difficulty in accessing and assessing the evidence for such programming. While NBI have been utilized in a wide array of human populations, no interventions have been reported among clinical health professions students. Aims. The objectives of this dissertation project were to review the existing body of NBI literature exploring health and wellbeing outcomes, and to conduct a feasibility trial of forest bathing, a type of NBI, among clinical students. Specifically, …

Investigating The Role Of Nudt7-Mediated Peroxisomal Coa Degradation In The Regulation Of Hepatic Coa Levels And Lipid Metabolism, Schuyler Dan Adams Vickers

Graduate Theses, Dissertations, and Problem Reports (ETD)

Coenzyme A (CoA) is a crucial cofactor required to support many essential metabolic processes, including fatty acid oxidation and synthesis, the TCA cycle, and bile acid synthesis. In order to support the proper functioning of these pathways, CoA levels within the cell must be properly regulated. The regulation of CoA levels is achieved through a balance between CoA synthesis and CoA degradation. While the regulation of CoA synthesis has been extensively characterized, the degradation of CoA has received less attention. As such, the contribution of CoA degradation to the regulation of metabolism is poorly understood. NUDT7 is a CoA-specific Nudix …

Addition Of Apple Pomace To Feeding Substrate On Growth, Development, And Survival Rates Of Yellow Mealworms (Tenebrio Molitor) And Characterization Of Their Proteins Extracted By A Ph-Shift Process, Michelle Duvall

Graduate Theses, Dissertations, and Problem Reports (ETD)

The weight changes, survival/mortality rates, and proximate compositions of Tenebrio molitor (yellow mealworm) larvae whose diets were supplemented with apple pomace were analyzed after a 4-week feeding study to examine the nutritional quality of the insects for use as a future potential food source. Going forward, the terms “mealworm(s)”, "yellow mealworm(s)”, “T. molitor” will be used interchangeably and will always refer to the same species. The mealworm larvae were randomly divided into two groups: in addition to standard worm chow, one group was given 15 g/week of non-nutritive water-storing polymer crystals and the other was given apple pomace …