Digital Commons Network^™

Transitions Between The Steps Of Group Iic Intron Forward And Reverse Splicing And Integration Into Dna, Claire Mae Smathers

Graduate Theses, Dissertations, and Problem Reports (ETD)

Group II introns are ancient ribozymes capable of self-excision and mobility into new genomic locations. As the ancestors of both the eukaryotic spliceosome and eukaryotic retroelements, these simple RNA machines serve as excellent models for understanding the mechanisms of both gene splicing and retroelement activity in eukaryotes. Although group II introns have been studied for decades, knowledge of the most unique and arguably evolutionarily relevant class of group II introns, IIC, is limited. Our knowledge is especially limited in terms of understanding how IIC introns mediate the transition between the two transesterification steps of forward splicing and reverse splicing into …

Behaviors, Contextual Influences, And Consequences: Relationships That Affect Student Decision-Making Of Academic Misconduct In College, Justine A L Burnett

Graduate Theses, Dissertations, and Problem Reports (ETD)

Academic misconduct on college campuses is not a new challenge for higher education institutions but an old problem that has changed considerably. Student demonstrations of academic misconduct behaviors have evolved, making it difficult for institutions to consistently keep well-informed on how students cheat to effectively respond to violations of academic integrity policies. This study investigates the relationships between misbehaviors, their associated consequences, and influences to prevent, respond to, and reduce academic misconduct at a large research-intensive university.

This quantitative study uses institutional academic misconduct reports between 2017 and 2020 and student surveys to examine significant relationships in the decision-making process …

Targeting Vulnerabilities In Cell State And Calcium Signaling For The Treatment Of Lung Cancer, Clark Jones

Graduate Theses, Dissertations, and Problem Reports (ETD)

Lung cancer remains the deadliest of all cancers due to the high mutational burden associated with the disease. Combating mutational drivers and drug resistance proves to be essential in the development of novel therapies to improve patient outcomes. A first-in-class cyclic peptide known as MTI-101 has been shown to induce necrotic cell death in a caspase independent manner. MTI-101 was derived from the linear peptide known as HYD1 that was found in a high throughput screen to block cell adhesion with the extracellular matrix. The compound was further optimized and cyclized to the currently used MTI-101 that was found to …

A Monte Carlo Simulation Of Rat Choice Behavior With Interdependent Outcomes, Michelle A. Frankot

Graduate Theses, Dissertations, and Problem Reports (ETD)

Preclinical behavioral neuroscience often uses choice paradigms to capture psychiatric symptoms. In particular, the subfield of operant research produces nested datasets with many discrete choices in a session. The standard analytic practice is to aggregate choice into a continuous variable and analyze using ANOVA or linear regression. However, choice data often have multiple interdependent outcomes of interest, violating an assumption of general linear models. The aim of the current study was to quantify the accuracy of linear mixed-effects regression (LMER) for analyzing data from a 4-choice operant task called the Rodent Gambling Task (RGT), which measures decision-making in the context …

Analysis Of Forensically Relevant Evidence Using Electrochemistry, Spectroscopy, And Mass Spectrometry Tools, Colby Edward Ott

Graduate Theses, Dissertations, and Problem Reports (ETD)

Forensic science relies on the use of multiple techniques in the assessment of evidence to increase the accuracy and reliability of the results. However, with the rapidly changing drug landscape due to the introduction of novel psychoactive substances, many traditional screening methods are no longer sensitive or selective enough for use. Additionally, many screening methods such as chemical color tests are prone to false positive and negative results and are subjective. Therefore, the goal of this dissertation was to develop a novel analytical scheme that can provide a more efficient, rapid, and sensitive method that will facilitate adoption in the …

The Discover Model: A Prescriptive Method For Instructional Tool Selection And Use In Seeking To Boost Instructor Immediacy And Social Presence In Online Courses, Lee O. Silverman

Graduate Theses, Dissertations, and Problem Reports (ETD)

Higher education faculty who teach online can face numerous challenges in providing optimal experiences for their students. Besides their potentially limited instructional design expertise and understanding of how to optimize technology to support learning, faculty may have difficulties in finding ways to make themselves be perceived as real people who are approachable, caring, and likeable due to online nature of the learning context. As a preliminary means to address these issues, the current study focused on the development of the DISCOVER Model, which was designed to provide higher education faculty with a framework to develop actionable plans in the creation …

Reinforcement Learning For Process Control: Applications To Energy Systems, Elijah Ballard Hedrick

Graduate Theses, Dissertations, and Problem Reports (ETD)

Reinforcement learning (RL) is a machine learning method that has recently seen significant research activity owing to its successes in the areas of robotics and gameplaying (Silver et al., 2017). However, significant challenges exist in the extension of these control methods to process control problems, where state and input signals are nearly always continuous and more stringent performance guarantees are required. The goal of this work is to explore ways that modern RL algorithms can be adapted to handle process control problems; avenues for this work include using RL with existing controllers such as model predictive control (MPC) and adapting …

Investigating The Mitochondrial Protein Mitoneet In C. Elegans Models Of Aging And Alzheimer's Disease, Jacob Ryan Boos

Graduate Theses, Dissertations, and Problem Reports (ETD)

Oxidative stress is an imbalance between reactive oxygen species production and elimination, favoring the former. Reactive oxygen species serve as important signaling molecules for physiological homeostasis. However, when produced in excess, these once important signaling molecules become detrimental, disrupting cellular functions, and ultimately leading to cell death. In aging, reactive oxygen species production is increased, accompanied with reductions in oxidative stress resistance, increasing the risk for developing age-related diseases including cardiovascular disease, cancer, stroke, and neurodegenerative diseases. The outer mitochondrial membrane iron-sulfur cluster containing protein mitoNEET (CISD1; gene CISD1) has shown to be a mediator of mitochondrial function and …

Performance Of Peltier Cooled Technology During Preconcentration Of Volatile Organic Compounds Analyzed By Gas Chromatography-Mass Spectrometry (Gc-Ms) Using Niosh Manual Of Analytical Methods (Nmam) 3900, Elizabeth Ann Fernandez

Graduate Theses, Dissertations, and Problem Reports (ETD)

Using evacuated canisters for whole-air sampling allows for sampling without specific knowledge of the environment and potential analytes. Evacuated canisters are able to tolerate concentrations ranging from parts per trillion (PPT) to parts per million (PPM). The U.S. Environmental Protection Agency (EPA) has used evacuated canisters for years to sample volatile organic compounds (VOCs) from ambient air using their method TO-15 (LeBouf, et al., 2019). The GC-MS system used for the development of NMAM 3900 used liquid nitrogen to cool sorbent traps in the preconcentrator. Liquid nitrogen allows for cooling of specified points below ambient temperature. An alternative to liquid …

Repercussions: The Pressure To Be Thin, Shuhan Liu

Graduate Theses, Dissertations, and Problem Reports (ETD)

My MFA thesis and supporting exhibition will highlight ceramics work I have created with the intention of dissecting the relationship between a soul and reality to convey the story of tension and anxiety and show it in a way that would let viewers identify with my discomfort and anxiety. The inspiration for my work came from the severe body anxiety that I faced that broke out during the three years of psychological stress caused by the pandemic. Using personal narratives as a starting point, I created object-based artwork that addresses the external pressures I feel to be skinny. As an …

Direct Ink Writing Printability – Ashby-Like Plots For Guided Design, Domenic Cipollone

Graduate Theses, Dissertations, and Problem Reports (ETD)

Direct Ink Writing (DIW), an extrusion-based 3D printing technique, offers a broad application space. As such, the technique continues to find use in biomedical, flexible electronic, ceramic, and energy device applications, among others. With this broad application space comes an expanding material library of inks with diverse rheological and microstructural properties. This begs the question: what constitutes a printable ink? How does one define printability? And how does one design for printability?

Researchers currently have a broad understanding of what constitutes a printable ink. However, time and time again, inks with unique rheological properties and formulations are printed. Currently, ink …

Assessment Of User Environmental Perceptions Associated With Gray's Reef National Marine Sanctuary, Marieke Johanna Lemmen

Graduate Theses, Dissertations, and Problem Reports (ETD)

Resource management in marine protected areas is confronted with the challenging conflict of use and conservation of resources. Increased visitation in marine environments has led to the need of management due to negative ecological and social impacts. Understanding environmental values, attitudes, and perceptions is important to the success of environmental protection. Using online surveys sent via Qualtrics asking questions regarding the users’ knowledge, attitudes, and perceptions of ocean resources, goods and services, this research is focusing on identifying user profiles and understanding their environmental perception associated with Gray’s Reef National Marine Sanctuary, an offshore marine protected area, and surrounding coastal …

Face Representation Learning And Its Applications: From Image Editing To 3d Avatar Animation, Xudong Liu

Graduate Theses, Dissertations, and Problem Reports (ETD)

Face representation learning is one of the most popular research topics in the computer vision community, as it is the foundation of face recognition and face image generation. Numerous representation learning frameworks have been integrated into applications in daily life, such as face recognition, image editing, and face tracking. Researchers have developed advanced algorithms for face recognition with successful commercial productions, for example, FaceID on the smartphone. The performance record on face recognition is constantly updated and becoming saturated with the help of large-scale datasets and advanced computational resources. Thanks to the robust representation in face recognition, in this dissertation, …

Multi-Task Neuromuscular Generalization And Changes Through The Lifespan, Hannah Delaney Carey

Graduate Theses, Dissertations, and Problem Reports (ETD)

Mobility in everyday life requires executing and shifting between a broad assortment of functional tasks and resisting disturbances that could cause falls. Though the importance of successfully performing a variety of functional tasks is recognized and incorporated in clinical assessments (e.g., the Timed-Up-and-Go Test, Berg Balance Scale), little is understood about the underlying neuromuscular control required, or how it changes with age. The neuromuscular control for functional tasks such as walking is typically studied in isolation, or with variations on the same task. Characterizing the coordination required to produce and shift between a wider variety of tasks and resist external …

Exploiting Modulation Of The Blood-Brain And Blood-Tumor Barrier Permeability By Translational Focused Ultrasound For Therapeutic Delivery To Cns Metastases, Tasneem A. Arsiwala

Graduate Theses, Dissertations, and Problem Reports (ETD)

Transcranial low-intensity focused ultrasound is a unique technology to modulate the integrity of tight endothelial junctions and transiently increase BBB/BTB permeability to enhance therapeutic delivery. Despite promising early studies, present literature lacks agreement on key experimental conditions, which restricts our knowledge and the technique's widespread translation. This dissertation first provides a critical review of the current gaps in knowledge regarding the universal use of LiFUS in preclinical and clinical use. We then identify key parameters for translational and predictable opening of the BBB using a 3T MRI coupled with a clinical device. Our investigation highlights that passive permeability of the …

Student Understanding Of The Definite Integral When Solving Calculus Volume Problems, Krista Bresock

Graduate Theses, Dissertations, and Problem Reports (ETD)

The concept of integration appears in many different scientific fields, and students’ understanding of and ability to use the definite integral in applications is important to success in their STEM (science, technology, engineering, and mathematics) classes. One of the first types of application problems that students encounter is finding the volume of a solid using the definite integral. How students approach these problems and how they use the definite integral to find volumes can have an impact on their future use and understanding of the definite integral.

This study involves a deep and thorough investigation of how ten students understand …

Bottleneck Management Through Strategic Sequencing In Smart Manufacturing Systems, Sayantee Roy

Graduate Theses, Dissertations, and Problem Reports (ETD)

Nowadays, industries put a significant emphasis on finding the optimum order for carrying out jobs in sequence. This is a crucial element in determining net productivity. Depending on the demand criterion, all production systems, including flexible manufacturing systems, follow a predefined sequence of job-based machine operations. The complexity of the problem increases with increasing machines and jobs to sequence, demanding the use of an appropriate sequencing technique. The major contribution of this work is to modify an existing algorithm with a very unusual machine setup and find the optimal sequence which will really minimize the makespan. This custom machine setup …

System Development Of An Unmanned Ground Vehicle And Implementation Of An Autonomous Navigation Module In A Mine Environment, Jonas Amoama Bredu Jnr

Graduate Theses, Dissertations, and Problem Reports (ETD)

There are numerous benefits to the insights gained from the exploration and exploitation of underground mines. There are also great risks and challenges involved, such as accidents that have claimed many lives. To avoid these accidents, inspections of the large mines were carried out by the miners, which is not always economically feasible and puts the safety of the inspectors at risk. Despite the progress in the development of robotic systems, autonomous navigation, localization and mapping algorithms, these environments remain particularly demanding for these systems. The successful implementation of the autonomous unmanned system will allow mine workers to autonomously determine …

Implementing Trauma-Informed Care Through A Multi-Agency Learning Collaborative: A Theory-Driven Analysis Of Outcomes And Sustainability, Mira D H Snider M.S.

Graduate Theses, Dissertations, and Problem Reports (ETD)

Learning collaboratives (LCs) are often used in large-scale implementation initiatives to promote evidence-based practice across provider networks. Although the outcomes and stakeholder perspectives of many LCs have been documented, support for the effectiveness of LCs is equivocal, and the means by which LCs achieve long-term improvements in clinical care are not understood. The current study investigated outcomes and sustainability of a multi-agency LC for implementing trauma-informed care in 23 rural Pennsylvania counties. Changes in outcomes (i.e., trauma symptom screening, trauma-informed care training attendance, clinician confidence with using trauma informed-care, utilization of trauma-related diagnostic codes, retention in service, service unit density) …

Expert Witnesses And Arbitration Outcomes: Insights From Public Procurement Contracts, Bryan Mccannon, Claudio Detotto, Marco Vannini, Riccardo Marselli

Faculty & Staff Scholarship

We explore the use of expert witnesses in arbitration proceedings by analyzing public procurement contract disputes in Italy. Balancing cost with accuracy, participants to a contract select arbitration when speedy dispute resolution is valued highly. Arbitration mechanisms tend to give arbitrators discretion in how to proceed. Consequently, principal-agent problems can arise. Using an IV approach, we show that the use of an expert witness causes a slowing down of the case resolution, without having an effect on the outcome of the dispute nor resolving uncertainty as measured by unanimous decisions by the panel of arbitrators.

Save To Win: Using Contests To Promote Savings, Bryan Mccannon, Zachary Rodriguez, Roman Sheremeta

Faculty & Staff Scholarship

We ask whether linking savings accounts to contests can promote saving. We do this by offering contest-linked accounts to individuals in rural Uganda where poverty is a serious problem. Our design builds off of results in experimental economics documenting excessive competitiveness in contests, with the goal of harnessing this behavior for the good. We find that, properly designed, we encourage savings beyond both pre-treatment levels and the control group. We explore reasonable heterogeneous treatment effects and document long lasting impacts on wealth.

District Attorney Compensation And Performance, Bryan Mccannon, Morgan Stockham, Gregory Deangelo

Faculty & Staff Scholarship

Does prosecutor pay impact performance? We attempt to identify the causal effect of wages on a prosecutor’s effort by studying a large (41%), exogenous salary increase for district attorneys in New York state. We measure the performance of prosecutors by the likelihood that a conviction is upheld when appealed. If the efficiency wage theory accurately explains non-market actor behavior, then the exogenous wage shock should entice better performance. Alternatively, if individuals who hold public office are motivated primarily by an intrinsic desire to carry out their office duties to the best of their ability rather than strictly financial compensation, then …

Aacsb Accreditation And Student Demand, Bryan Mccannon, Katherine Starr, Marisa Cameron

Faculty & Staff Scholarship

We ask whether AACSB accreditation has a meaningful impact on university admissions. To do this, we explore 16 U.S. institutions which first achieved this certification recently. We, first, document a modest, but nonzero, impact on university-wide undergraduate applications, without any changes in first-year enrollment, price, or quality of the incoming student body. Restricting attention to business schools, while initial evidence suggests that the accreditation is associated with a decrease in enrollments, we show that this is complicated by non-parallel trends prior to accreditation. Compared to their comparison institutions, universities who seek out accreditation were experiencing flatter business enrollments. Correcting for …

The Right To Counsel: Criminal Prosecution In 19th Century London, Bryan Mccannon, Zachary Porreca

Faculty & Staff Scholarship

Exploiting a novel data set of criminal trials in 19th century London, we evaluate the impact of an accused’s right to counsel on convictions. While lower-level crimes had an established history of professional representation prior to 1836, individuals accused of committing a felony did not, even though the prosecution was conducted by professional attorneys. The Prisoners’ Counsel At of 1836 remedied this and first introduced the right to counsel in common law systems. Using a difference-in-difference estimation strategy we identify the causal effect of defense counsel. We find the surprising result that the professionalization of the courtroom lead to an …

The “Ebb And Flow” Of Documentation: Does The Transition Between Two Electronic Medical Records Systems Affect Emergency Department Efficiency?, Michelle Angeline Md, Erica B. Shaver Md, Christopher Kiefer Md, Kimberly D. Quedado Phd, Melinda Sharon, Stephen Davis Phd, Kyle Hurst Md, Christopher S. Goode Md, Thomas C. Marshall Md

Faculty & Staff Scholarship

Background: Electronic Medical Record (EMR) systems are electronic databases for compiling patient records. As healthcare networks expand, it is critical for providers to have access to patient data more broadly. As a result individual healthcare facilities must adjust to enterprise wide EMRs.

Objective: This study examined the operational effects of transitioning from an Emergency Department (ED) specific EMR to an enterprise wide EMR by evaluating throughput metrics in a community ED.

Methods: During a 6-month transition period (July-December 2017) in a community-based, academic ED located in North Central West Virginia, length of stay (LOS) and the following operational metrics were …

Alex Lyon & Son, Sales Managers & Auctioneers V. Leach: Auction Contracts, Bidder Qualifications, Offer And Acceptance, Waiver, And The Fallacy Of Treating All Bidders The Same, George A. Michak

West Virginia Law Review Online

In Alex Lyon & Son, Sales Managers & Auctioneers v. Leach, 844 S.E.2d 120 (W. Va. 2020), the Supreme Court of Appeals of West Virginia grappled with the contractual relationships among participants in an auction transaction and rendered an opinion that (i) misstates and misaligns the rights and obligations among auctioneers, sellers, bidders, and buyers, (ii) impedes the ability of an auctioneer to reasonably control the conduct of an auction, and (iii) threatens to artificially circumscribe the prerogative of sellers and auctioneers to assume greater risks relative to certain bidders in an effort to expand the bidder pool in …

Exploring Dft+U Parameter Space With A Bayesian Calibration Assisted By Markov Chain Monte Carlo Sampling, Pedram Tavadze, Reese Boucher, Guillermo Avendaño-Franco, Keenan X. Kocan, Sobhit Singh, Viviana Dovale-Farelo, Wilfredo Ibarra-Hernández, Matthew B. Johnson, David S. Mebane, Aldo H. Romero

Faculty & Staff Scholarship

The density-functional theory is widely used to predict the physical properties of materials. However, it usually fails for strongly correlated materials. A popular solution is to use the Hubbard correction to treat strongly correlated electronic states. Unfortunately, the values of the Hubbard U and J parameters are initially unknown, and they can vary from one material to another. In this semi-empirical study, we explore the U and J parameter space of a group of iron-based compounds to simultaneously improve the prediction of physical properties (volume, magnetic moment, and bandgap). We used a Bayesian calibration assisted by Markov chain Monte Carlo …

A Human Being Wrote This Law Review Article: Gpt-3 And The Practice Of Law, Amy B. Cyphert

Law Faculty Scholarship

Artificial intelligence tools can now “write” in such a sophisticated manner that they fool people into believing that a human wrote the text. None are better at writing than GPT-3, released in 2020 for beta testing and coming to commercial markets in 2021. GPT-3 was trained on a massive dataset that included scrapes of language from sources ranging from the NYTimes to Reddit boards. And so, it comes as no surprise that researchers have already documented incidences of bias where GPT-3 spews toxic language. But because GPT-3 is so good at “writing,” and can be easily trained to write in …

Algebra, Wvu Mathematics Department

M.S. Advanced and Ph.D. Entrance Exams

No abstract provided.

Information Avoidance And Celebrity Exposure: The Effect Of "Magic" Johnson On Aids Diagnoses And Mortality In The U.S., Alexander Cardazzi, Joshua C. Martin, Zachary Rodriguez

Economics Faculty Working Papers Series

No abstract provided.