Digital Commons Network^™

Comparison Of Antimicrobials And Delivery Methods On The Inactivation Of Listeria Monocytogenes On Apples, Rebecca B. Stearns

Graduate Theses, Dissertations, and Problem Reports (ETD)

Introduction: Due to multi-state outbreaks of listeriosis on apples in 2015, 2017, and 2019, Listeria Monocytogenes (L. monocytogenes) has been deemed an emerging, under researched pathogen by the USDA-NIFA. Therefore, this study evaluated the efficacy of SaniDate 5.0®’s (SD) ability to inactivate L. Monocytogenes on apples when delivered through electrostatic sprayer (ES), garden sprayer (GS), and dip methods.

Methods: Organic apples were dip-inoculated with L. monocytogenes for 5 min and then dried for 10 min. Once dried, the inoculated apples were untreated (control), sprayed with water only, or treated with SD, 0.0064, 0.1, 0.25 and 0.50% for 20 …

Assessment Of Quechers Extraction Protocol For The Lc-Ms/Ms Detection Of Emerging Fentanyl Analogs In Biological Specimens For Clinical And Forensic Purposes, Kylea M. Morris

Graduate Theses, Dissertations, and Problem Reports (ETD)

Drug abuse within the United States is a dynamic and pervasive issue that affects the quality and life expectancy of our society. One of the major drug classes that are constantly utilized is opioids. Opioids are prescribed for pain management in patients suffering from cancer and other medical illnesses. Long-term use of opioids could lead to tolerance in humans, which poses a risk of reaching fatal overdose levels. Unfortunately, opioids are abused recreationally, and in some instances, individuals are unaware of the presence of dangerous synthetic drugs like fentanyl, hidden in materials such as heroin, cocaine, oxycodone, and methamphetamine. Recent …

Psychosocial Influences On And Behavioral Characteristics Of Young Adult Food Addiction, Rachel A. Wattick

Graduate Theses, Dissertations, and Problem Reports (ETD)

Introduction. Research on food addiction has increased in recent years, but there are few studies focusing on college-attending young adults, who may be at risk of developing food addiction due to the stress of the college environment. Additionally, causes and characteristics of food addiction are under-explored.

Aim. This dissertation aims to 1) determine the impact of adverse childhood experiences (ACEs) and other early life influences on the development of food addiction in college-attending young adults and 2) determine the psychosocial and behavioral characteristics of young adults with food addiction.

Methods. A sequential explanatory mixed-methods design was used for aims 1 …

Cross-Kingdom Interactions Shape Soil Biogeochemistry In Natural And Agricultural Ecosystems, Jennifer Lynne Kane

Graduate Theses, Dissertations, and Problem Reports (ETD)

Microorganisms influence life on earth in innumerable ways, including in medical, industrial, environmental, and agricultural contexts. Given the increasingly apparent consequences of climate warming, interest in how to better predict and manage Earth’s carbon sinks has never been greater. Soil, the largest terrestrial carbon sink, harbors an incredibly taxonomically and functionally diverse microbial community. These soil-dwelling microbes govern the fate of soil carbon and nutrients by cycling organic matter as they live, grow, and die. It has only been relatively recently that technological advancement has allowed for in-depth surveys of the vast diversity of soil microbes. High throughput analytical capabilities …

Influences Of Disrupted Circadian Rhythms On Stroke Outcome, Jennifer A. Liu

Graduate Theses, Dissertations, and Problem Reports (ETD)

The circadian system is composed of a subset of temporal oscillators that function through a transcriptional and post-translational molecular and functional negative feedback loop cycling approximately every 24 hours. The central clock located in the suprachiasmatic nucleus is responsible for entrainment using light as the key timekeeper (zeitgeber); it is responsible for synchronizing and optimizing physiological behavior and function to the environment. Exogenous information, such as day length and light-dark cycles, provide critical temporal cues for adjusting to environmental conditions. Proper alignment to natural light dark cycles and circadian rhythms is optimal for vital health, fitness, and survival in organisms. …

Investigating The Interplay Of The Immune System, Skin Barrier, And Microbiome Following Dermal Exposure To Triclosan, Rachel Baur

Graduate Theses, Dissertations, and Problem Reports (ETD)

Triclosan is an antimicrobial chemical used in healthcare products that are applied to the skin. However, exposure to triclosan has been positively associated with food allergy, aeroallergy, and asthma severity in humans. In mice, dermal triclosan exposure, although not directly sensitizing, has been shown to augment the allergic response in a model of asthma. Furthermore, exposure to triclosan on mouse skin has been demonstrated to be immunomodulatory through the activity of thymic stromal lymphopoietin and S100 calcium-binding protein A8. The skin barrier and microbiome are known to interact with the immune system and disruptions in barrier integrity and the microbiome …

Integration Of Fisheries Ecology With The Assessment Of Microplastic Consumption In Black Bass In The Upper Ohio River Drainage, Kaylyn Zipp

Graduate Theses, Dissertations, and Problem Reports (ETD)

This thesis explores the dynamics of microplastic consumption of black bass (smallmouth, Micropterus salmoides, largemouth, M. dolomieu, and spotted bass, M. punctulatus) in the Monongahela and Kanawha River and explores dietary characteristics in the Ohio, Monongahela, and Kanawha River. The first chapter serves as an introduction and synthesis of microplastic consumption, fisheries ecology, and regional information. The second chapter quantifies microplastic consumption in the Monongahela River and explores spatial variation within and between species by pool and seasonal effects. The third chapter consists of exploratory Quasi Poisson modeling of total microplastics in the Point Marion pool, Monongahela River, WV using …

Evaluating Muskellunge (Esox Masquinongy) Catch-And-Release Mortality At Elevated Summer Water Temperature, Ian Taylor Booth

Graduate Theses, Dissertations, and Problem Reports (ETD)

Angling for Muskellunge (Esox masquinongy) has become widespread and increasingly popular throughout the fish’s range. Current management strategies for Muskellunge include high minimum harvest lengths (>1016mm), closed seasons, and catch-and-release regulations. Due to these strategies and angler sentiments, up to 97% of Muskellunge caught today are released by anglers assuming they will live and be caught again in the future. Previous research on catchand-release mortality in Muskellunge has suggested relatively low mortality rates (0%-5%). However, these studies were all conducted within the fish’s thermal optimum and generally at water temperatures25°C during the summer months, representing a need to evaluate …

Integration Of Spectroscopic And Mass Spectrometric Tools For The Analysis Of Novel Psychoactive Substances In Forensic And Toxicology Applications, Travon Cooman

Graduate Theses, Dissertations, and Problem Reports (ETD)

Analytical methods aiming for the detection of novel psychoactive substances are continuously revised due to their utility in the seized drug and toxicology realms. One method frequently employed for the preliminary identification of illicit materials is portable Raman spectroscopy. Even when a substance in possession of an offender is identified, conclusive evidence that it may have been consumed requires additional confirmatory work and further toxicological evaluation of a biological specimen. Many times, the substance consumed may not be detected in the analyzed specimen due to its extensive metabolism. It is therefore challenging to rule out the identity of the drug …

Performance Of Sensor Fusion For Vehicular Applications, Nikola Janevski

Graduate Theses, Dissertations, and Problem Reports (ETD)

Sensor fusion is a key system in Advanced Driver Assistance Systems, ADAS. The perfor-
mance of the sensor fusion depends on many factors such as the sensors used, the kinematic
model used in the Extended Kalman Filter, EKF, the motion of the vehicles, the type of
road, the density of vehicles, and the gating methods. The interactions between parameters
and the extent to which individual parameters contribute to the overall accuracy of a sensor
fusion system can be difficult to assess.
In this study, a full-factorial experimental evaluation of a sensor fusion system based
on a real vehicle was performed. …

Population Characteristics Of Three Black Bass Species In The Upper Ohio River Drainage, Levi Foster Brown

Graduate Theses, Dissertations, and Problem Reports (ETD)

In West Virginia, species of black bass are economically and ecologically important and support popular fisheries. In the state’s large, navigable river systems, recent information suggests that anglers consider these fisheries diminished relative to their historic stocks. This study sought to describe the population characteristics of Largemouth, Smallmouth, and Spotted Bass in the upper Ohio River drainage and evaluate possible management actions. Low relative abundances coupled with poor growth estimates for Largemouth and Spotted Bass suggests that a suite of environmental and anthropogenic factors may be reducing the quality of their recreational fisheries. Conversely, demographic estimates produced for Smallmouth Bass …

Landmark Enforcement And Principal Component Analysis For Improving Gan-Based Morphing, Samuel W. Price

Graduate Theses, Dissertations, and Problem Reports (ETD)

Facial Recognition Systems (FRSs) are a key target for adversaries determined to circumvent security checkpoints. Morph images threaten FRS by presenting as multiple individuals, allowing an adversary to swap identities with another subject. Although morph generation using generative adversarial networks (GANs) results in high-quality morphs without possessing the spatial artifacts caused by landmarkbased methods, there is an apparent loss in identity with standard GAN-based morphing methods. In this thesis, we examine landmark-based and GAN-based morphing methods to fuse the advantages of both methodologies. We propose a novel StyleGAN2 morph generation technique by introducing a landmark enforcement method. Considering this method, …

Clitic Doubling: The Case Of Nariño Andean Spanish, Jessica Paola Jurado Eraso

Graduate Theses, Dissertations, and Problem Reports (ETD)

This thesis examines the behavior of clitic doubling in Nariñense, a little-studied Andean Spanish variety spoken in the southwest of Colombia, and compares it to well-studied varieties such as standard Peninsular, Rioplatense, and other Andean Spanish varieties. Its aim is to present novel data about clitic doubling in order to shed more light into the extensive literature about this phenomenon in Spanish. Through spontaneous speech recordings obtained from thirty native speakers of Nariñense, 133 sentences involving clitic doubling were analyzed. Features such as agreement matching between the clitic and the doubled NP, use of the preposition a to introduce the …

Frayed Connections: How Long-Term Nitrogen Additions Disrupt Plant-Soil Interactions And The Carbon Cycle Of A Temperate Forest, Brooke A. Eastman

Graduate Theses, Dissertations, and Problem Reports (ETD)

Forests are expected to mitigate some of the negative effects of climate change by sequestering anthropogenic carbon (C) from the atmosphere, but the degree to which they drawn down C will depend on the availability of key nutrients, such as nitrogen (N). There is a fair amount of uncertainty in the future of the forest C sink, mostly owing to the fate of soil organic matter (SOM) and soil heterotrophic respiration to future conditions. In N limited systems, plants allocate a significant amount of their photosynthate belowground for the acquisition of nutrients, but under conditions of chronic N deposition, plants …

Fasciotomies, Gabriel Bass

Graduate Theses, Dissertations, and Problem Reports (ETD)

This collection of poems follows the disjointed and never complete trajectory of personal recovery from trauma and alcoholism. The poems and their arrangement trouble the notion of a person ever being fixed, even as they make progress toward a better version of themselves. The collection illustrates how the past can impose itself on the present, creating dissonance and complicating the speaker’s understanding of time and recovery.

Amelioration Of Mitochondrial Bioenergetic Dysfunction In Diabetes Mellitus: Delving Into Specialized And Non-Specific Therapeutics For The Ailing Heart, Andrya Jean Durr

Graduate Theses, Dissertations, and Problem Reports (ETD)

Morbidity and mortality of the diabetic population is influenced by many confounding factors, but cardiovascular disease (CVD), remains the leading cause of death. Mitochondrial dysfunction is central in the development of cardiac contractile dysfunction, with decreased mitochondrial bioenergetic function, increased dependence on free fatty acid utilization, and a decrease in glucose utilization having been shown to contribute to contractile dysfunction. Strategies targeting the amelioration of mitochondrial bioenergetic function are attractive for limiting diabetes-induced heart failure, and preserving health-span. The goals of this dissertation were to assess two mitochondrial-centric approaches for the amelioration of mitochondrial and cardiac contractile dysfunction in diabetes …

Incidence Of Cancer, Depression, And Economic Burden Of Prescription Nsaids Among Older Adults With Osteoarthritis: Statistical And Machine Learning Approaches, Nazneen Fatima Mohammed Umer Shaikh

Graduate Theses, Dissertations, and Problem Reports (ETD)

Understanding the role of chronic inflammation among older adults is critical because of its implications on chronic inflammatory conditions as well as its interaction with anti-inflammatory medications. Osteoarthritis (OA) is a heterogeneous multi-faceted joint disease with multi-tissue involvement of varying severity, and increasing evidence demonstrates a key role of chronic inflammation in its pathogenesis. Approximately 30 million adults in the United States have OA, and it is reported that a large proportion (43%) of them are older than 65 years. OA is typically treated using non-steroidal anti-inflammatory drugs (NSAIDs) for minimizing pain and reducing inflammation. NSAIDs have consistently shown clinically …

Navigating Abrupt Shifts In Contextual Discourses During A Pandemic And Lack Of Mentorship: A Preservice Teacher’S Journey Developing Critical Pedagogical Discourses, Casey L. King

Graduate Theses, Dissertations, and Problem Reports (ETD)

This study is centered on one English language arts (ELA) preservice teacher’s development of her critical pedagogical discourses (CPD) with the contextual discourses of a school placement for preservice teaching and later shift to a full-time teacher before the placement was complete during a pandemic and in the midst of implementing online learning. Data is drawn from a 4-month interpretive qualitative case study that included classroom observations and semi-structured interviews. The objective for this study included how a preservice teacher uses their beliefs and identity about instruction amid changing contextual discourses in a pandemic and with a lack of mentorship. …

Utilizing The K18-Hace2 Mouse Model To Develop Protective Covid-19 Vaccines, Ting Y. Wong

Graduate Theses, Dissertations, and Problem Reports (ETD)

The ongoing Coronavirus Disease 2019 (COVID-19) pandemic is caused by the respiratory virus Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2). Similar to other respiratory viruses, SARS-CoV-2 is transmitted through inhalation of respiratory droplets and aerosols from infected individuals. Once inhaled, SARS-CoV-2 utilizes the receptor binding domain (RBD) on the spike protein to bind to human Angiotensin Converting Enzyme 2 (hACE2) receptor to gain entrance into host cells to begin viral replication. SARS-CoV-2 infection can result in mild to severe cases of COVID-19 ranging from asymptomatic infections, cold or flu like symptoms to respiratory failure. The onset of the pandemic in …

Associations Between Self-Reported Awe And Heart Rate, Laura E. Bernstein

Graduate Theses, Dissertations, and Problem Reports (ETD)

Fredrickson’s (1998, 2013) broaden and build theory states that positive emotions can expand our social and cognitive awareness and provide health benefits. Some positive emotions, such as awe, may operate differently. We sought to understand how subjective reports of awe, induced through videos, correlate with HR. We know that HR tends to decrease with age (Umetani et al., 1998), individuals have less physiological reactivity as they age (Blanchard-Fields, 2007), and older adults report more positive emotions than negative emotions (Mroczek & Kolarz, 1998). Given these findings, we were interested in understanding whether there were age differences in self-reported awe and …

Deep Radio Observations And The Role Of The Cosmic Web In Galaxy Evolution, Nicholas M. Luber

Graduate Theses, Dissertations, and Problem Reports (ETD)

A current open question in the evolution of galaxies, is what are the physical mechanisms that cut off galaxies from their primordial gas reservoirs, resulting in the end of their star-formation capabilities? Recent observational programs have shown that the properties of galaxies show dependencies on their placement within the large-scale structure (LSS) of the universe. These observations have motivated recent developments in theoretical work that have shown how a galaxy's interaction with the LSS may impact its connection to primordial gas supply, and ability to continue to accrete gas, the fundamental ingredient in star-formation.

In order to investigate the role …

Transitions Between The Steps Of Group Iic Intron Forward And Reverse Splicing And Integration Into Dna, Claire Mae Smathers

Graduate Theses, Dissertations, and Problem Reports (ETD)

Group II introns are ancient ribozymes capable of self-excision and mobility into new genomic locations. As the ancestors of both the eukaryotic spliceosome and eukaryotic retroelements, these simple RNA machines serve as excellent models for understanding the mechanisms of both gene splicing and retroelement activity in eukaryotes. Although group II introns have been studied for decades, knowledge of the most unique and arguably evolutionarily relevant class of group II introns, IIC, is limited. Our knowledge is especially limited in terms of understanding how IIC introns mediate the transition between the two transesterification steps of forward splicing and reverse splicing into …

Bitter Sweet, Hanna M. Kesty

Graduate Theses, Dissertations, and Problem Reports (ETD)

This written document is the accompanying thesis for my Master of Fine Arts Exhibition, Bitter Sweet. The exhibition featured traditional framed prints with sculptural accents that reveal brutally honest negative personal experiences, interactions, and emotions, paired with delicately cute aesthetics. My work embodies the necessity of personal artistic expression to process years of emotional repression. The prints and sculptures in the exhibition focus around a collection of insults and harsh comments coupled with feelings of loneliness and isolation. I allow personal vulnerability to show, to reinforce that these moments have molded and shaped who I am and will continue to …

Detecting Adverse Drug Events Using A Deep Neural Network Model, Saminur Islam

Graduate Theses, Dissertations, and Problem Reports (ETD)

Adverse drug events represent a key challenge in public health, especially with respect to drug safety profiling and drug surveillance. Drug-drug interactions represent one of the most popular types of adverse drug events. Most computational approaches to this problem have used different types of drug-related information utilizing different types of machine learning algorithms to predict potential interactions between drugs. In this work, our focus is on the use of genetic information about the drugs, in particular, the protein sequence and protein structure of drug protein targets to predict potential interactions between drugs. We collected information on drug-drug interactions (DDIs) from …

Black Walnut Plantations In West Virginia: Maximizing Financial Returns Through Decision Modeling And Cash Flow Analysis, Erin Duffy Shaw

Graduate Theses, Dissertations, and Problem Reports (ETD)

The purpose of this study was to identify the management strategies that lead to maximum financial returns from a black walnut plantation. To evaluate a selection of plantation establishment scenarios, thinning treatments, and product objectives, an Excel-based black walnut financial model was updated and revised. Key updates to the model included incorporating three cash flows for 1) the collection and wholesale of black walnut sap, 2) producing black walnut syrup, and 3) leasing black walnut trees for tapping. Additionally, outputs from the Forest Vegetation Simulator were integrated into the model’s growth and yield projections as a means of more accurately …

Passive Self-Ligating Brackets Vs. Conventional Brackets: Is There A Difference In The Transverse Dimension? A Systematic Review And Meta-Analysis, Justin Scott Tenbrook

Graduate Theses, Dissertations, and Problem Reports (ETD)

Background and Objectives:

Self-ligating brackets have risen in popularity in clinical orthodontics, but many claims made by their manufacturers have been made without substantial evidence. Any differences among the bracket types are very small when consulting new peer reviewed literature testing their differences. Nevertheless, self-ligating brackets are still purported to be able to increase the dental transverse dimension more effectively than conventional brackets. In 2018, Yang et al. performed a systematic review on the transversal changes, space closure, and efficiency of conventional and self-ligating appliances. Since then, there were several clinical trials published in the literature comparing the mandibular arch …

Eco-Material Rifts In South Asian Anglophone Fiction, Muhammad Manzur Alam

Graduate Theses, Dissertations, and Problem Reports (ETD)

I examine how South Asian Anglophone fiction represents the evolution and derangement of postcolonial ecologies, especially how unrelenting colonial and capitalist interventions affect the symbiotic relationship between subaltern people and nonhuman entities. The conceptual methodology develops from Karl Marx’s theory of “metabolic rift,” which illustrates how capitalist exportation of crops causes loss of important soil nutrients because the nutrients are consumed in distant locations and not returned to the original soil. My concept of “eco-material rifts” extends Marx’s idea to contend that the “rifts” have grown into more complicated and difficult to remediate modes of material rifts today. I scrutinize …

Genetic Underpinnings Of Novel Trait Development In A Euwallacea-Fusarium Mutualism, Elaina J. Spahr

Graduate Theses, Dissertations, and Problem Reports (ETD)

Evolutionary Developmental Biology seeks to answer fundamental questions about the mechanisms underlying the evolution and innovation towards increasing structural complexity within the body plan. The ambrosia symbiosis, a polyphyletic group of xylem-feeding beetles, provides a wealth of diversity in novel pouch-like structures called mycangia. This diversity could serve as a rich model for understanding how mutualisms may prompt structural innovation and diversification in a host organism. The ambrosia symbiosis has become a fast-growing research subject in entomology and forest pathology but has yet to experience the same attention under the lens of evolutionary developmental genetics.

Development of mycangia was examined …

The Web And The Blockbuster Poster: An Examination Of The Impact Of Streaming Services On Movie Advertising, Holden Edward Strausser

Graduate Theses, Dissertations, and Problem Reports (ETD)

The year 2020 brought a widespread closure of movie theaters, exacerbating a trend wherein streaming services gained an increasing importance in movie distribution. This study examines how the changing nature of the motion picture distribution market after the advent of the streaming service is reflected in movie advertising, specifically the movie poster. The research uses a content analysis methodology to compare film posters across time, from before the beginning of streaming services to the arrival of the 2020 COVID-19 pandemic in the United States. Analysis is derived from Uses and Gratifications theory and scholarship on star power. The research shows …

Exploring How Children Use Their Everyday Thinking To Construct Scientific Explanations Of The Natural World, Ashley Nicole Kooken

Graduate Theses, Dissertations, and Problem Reports (ETD)

The purpose of this qualitative grounded theory study was to describe and explain children’s thinking present in multiple related contexts surrounding a single Earth Space disciplinary core idea in order to provide insight on whether and how children’s everyday thinking forms the basis for learning in science classrooms. As most previous work on children’s thinking focuses more on what ideas and thinking are present within specific science learning contexts, there is a knowledge gap as to how children’s everyday ideas across multiple contexts provide the basis from which they construct a deeper conceptual understanding about scientific phenomenon in the world …