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The Effectiveness Of Video Education On Pre-Operative Parental Knowledge And Anxiety, Terri Marcischak Jan 2017

The Effectiveness Of Video Education On Pre-Operative Parental Knowledge And Anxiety, Terri Marcischak

Graduate Theses, Dissertations, and Problem Reports (ETD)

Pre-operative anxiety is a common and anticipated response to patients expecting to undergo anesthesia in preparation for surgery. Pre-operative parental anxiety can lead to increased anxiety in children, which is associated with physiologic alterations in the child. Pre-operative video education is an inexpensive method to increase knowledge and decrease pre-operative parental anxiety related to the perioperative process. The first goal of this project was to evaluate the effectiveness of a pre-operative video in increasing knowledge of the perioperative experience in parents of children anticipating surgery by viewing a pre-operative video. Parental knowledge was measured before and after viewing the pre-operative …

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Proteomic And Metabolomic Analyses Of Biological Systems With Liquid Chromatography Tandem Mass Spectrometry And Ion Mobility Mass Spectrometry, Megan M. Maurer Jan 2017

Proteomic And Metabolomic Analyses Of Biological Systems With Liquid Chromatography Tandem Mass Spectrometry And Ion Mobility Mass Spectrometry, Megan M. Maurer

Graduate Theses, Dissertations, and Problem Reports (ETD)

Proteomic and metabolomic analyses provide information about altered metabolic processes in plants and animals. This information can be used to assess the impact of toxicant exposure or diseases on biological systems. In this dissertation, development of an ion mobility spectrometry - mass spectrometry (IMS-MS) strategy has been evaluated in addition to utilizing traditional liquid chromatography tandem mass spectrometry (LC-MS/MS) techniques for metabolite and protein analysis. Traditional LC-MS/MS techniques have been applied to the study of: 1) the molecular mechanisms responsible for altered microvasculature function as a result of pulmonary TiO2 exposure in rats; and 2) the effectiveness of intracerebroventricular (ICV) …

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Role Of Perivascular Adipose Tissue In Vascular Pathology And The Therapeutic Effect Of Exercise, Evan Ray Devallance Jan 2017

Role Of Perivascular Adipose Tissue In Vascular Pathology And The Therapeutic Effect Of Exercise, Evan Ray Devallance

Graduate Theses, Dissertations, and Problem Reports (ETD)

Aortic compliance is important for dampening pulsatile flow and delivery of continuous flow to the periphery. Aortic compliance is regulated by extracellular matrix composition and endothelial derived nitric oxide. Disruption of aortic endothelium or extracellular matrix can lead to the development of aortic stiffness an independent risk factor of cardiac events and mortality. Over the past decade perivascular adipose tissue (PVAT) surrounding the vasculature has come to light as an important regulator of artery function. The main focus of this dissertation is to evaluate the role PVAT surrounding the aorta in mediating endothelial relaxation in health and disease. Specifically, metabolic …

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Effects Of Differential Drl Exposure On Interval Timing: Information Vs. Inhibition, Matthew L. Eckard Jan 2017

Effects Of Differential Drl Exposure On Interval Timing: Information Vs. Inhibition, Matthew L. Eckard

Graduate Theses, Dissertations, and Problem Reports (ETD)

Recent investigations focusing on interventions to improve self-controlled choice have centered upon timing processes. However, speculation still remains regarding the mechanism of these interventions. To investigate how these interventions have their effects, timing in mice was assessed using an 18-s peak procedure (18-s FI trials; 54-s peak trials). During an intervention phase, mice in three treatment groups experienced differential reinforcement of low rate (DRL) schedules of reinforcement. A control group received continued exposure to the peak procedure. After 36 DRL sessions, timing was reassessed in the peak procedure. In contrast to previous reports, the DRL intervention resulted in less precise …

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Child-Care Directors' Years Of Experience In Relation To The Activities Subscale Score On The Early Childhood Environment Rating Scale-R, Angelica Everetts Jan 2017

Child-Care Directors' Years Of Experience In Relation To The Activities Subscale Score On The Early Childhood Environment Rating Scale-R, Angelica Everetts

Graduate Theses, Dissertations, and Problem Reports (ETD)

This study focused on examining the relationship between the child-care directors' years of experience in relation to the Activities subscale on the Early Childhood Environment Rating Scale-R. The data used in the study was from the Quality Rating and Improvement for West Virginia Child-Care grant, which was funded by the West Virginia Department of Health and Human Resources. The participants included 175 4-year-old classrooms across West Virginia. The Early Childhood Environment Rating Scale contains 43 items across seven subscales. This scale was used to measure the activities score of the 4-year-old classrooms. There was no significant relationship found between the …

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Dual Targeting Of The Mesenchymal And Amoeboid Pathways: A Viable Therapy Against Metastatic Breast Cancer, Brandon C. Jones Jan 2017

Dual Targeting Of The Mesenchymal And Amoeboid Pathways: A Viable Therapy Against Metastatic Breast Cancer, Brandon C. Jones

Graduate Theses, Dissertations, and Problem Reports (ETD)

Metastasis from the primary tumor site is the major cause of death in breast cancer, and acquisition of migration capacity is a key element for successful metastasis. Recently, it was found that expression of NEDD9, a focal adhesion associated scaffolding protein, could be used to switch between two types of single cell movement known as mesenchymal and amoeboid. Control of this "switch" can potentially open up new avenues of treatment against cancer metastasis. Simultaneous inhibition of both types of single cell movement may prevent cancer migration, thus decreasing cancer spreading and increasing survival of cancer patients. For example, forcibly pushing …

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High Touch, Andrew Kellner Jan 2017

High Touch, Andrew Kellner

Graduate Theses, Dissertations, and Problem Reports (ETD)

This written thesis is the supporting documentation for High Touch, my Master of Fine Arts thesis exhibition. The exhibition seeks to define my personal expression of beauty through domestic pottery that seeks to both elevate and celebrate mundane life experiences.;The research underpinning my thesis explores the connections between object and user. The use of pottery, particularly in the home, has the ability to breed familiarity, consolation, comfort and security. It is only when pots are put into service within the household that an affinity for the object is achieved and its unique character is revealed. These moments cannot be codified …

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Ripple Effect Mapping Of Youth & Adult Dyad Pairs Demonstrating Community Impact From Icook 4-H Intervention, Sina J.E. King Jan 2017

Ripple Effect Mapping Of Youth & Adult Dyad Pairs Demonstrating Community Impact From Icook 4-H Intervention, Sina J.E. King

Graduate Theses, Dissertations, and Problem Reports (ETD)

Introduction: Creating effective dissemination and implementation tools within community based, obesity prevention research is needed to bridge the current science to practice gap. Ripple Effect Mapping (REM) using Community Capitals Framework (CCF) is a community impact evaluation tool that is hypothesized to positively capture activity during dissemination within community programs. Although REM is a proposed impact evaluation method, it has not been widely adopted within the research sector. In order to facilitate the translation of research into evidenced based practice, a stepwise process must be described and tested to determine efficacy and effectiveness, followed by a method to translate findings …

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Evaluating Steam Conditioning Manipulations And Grain Expressed Enzymes Effects On Feed Manufacturing Metrics, Feed Hygiene, Broiler Performance, And Bone Mineralization, John William Boney Jan 2017

Evaluating Steam Conditioning Manipulations And Grain Expressed Enzymes Effects On Feed Manufacturing Metrics, Feed Hygiene, Broiler Performance, And Bone Mineralization, John William Boney

Graduate Theses, Dissertations, and Problem Reports (ETD)

Animal well-being, evolving feed manufacturing regulatory programs, and consumer perception drive the continued need to provide safe feed to animals. Considering the performance benefits associated with feeding pelleted feed, the concepts of hygienic feed production and production of highly durable pellets may interact to benefit the broiler chicken. In addition to hygienic and physical attributes, poultry nutrition may be enhanced by cereal grains expressing enzymatic activity. These additives may provide additional opportunities to support dietary phosphorus (P) requirements in growing broilers, further contributing to improved animal welfare conditions in an economically favorable manner. In order to study the effects of …

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Novel Mechanisms Controlling The Expression Of The Antidiabetic Hepatokine Fibroblast Growth Factor 21, Kimberly M. Alonge Jan 2017

Novel Mechanisms Controlling The Expression Of The Antidiabetic Hepatokine Fibroblast Growth Factor 21, Kimberly M. Alonge

Graduate Theses, Dissertations, and Problem Reports (ETD)

The incidence of obesity has increased at an alarming rate during the past thirty years. Current therapies for the treatment and prevention of obesity and metabolic syndrome are ineffective and/or produce undesirable side effects. Fibroblast growth factor 21 (FGF21) is a liver derived hormone that mediates adaptive changes in hepatic glucose and lipid metabolism by elevating energy expenditure and reducing lipid storage. Pharmacological administration of FGF21 analogs in both animal and human models induces striking reversals of obesity and type 2 diabetes. However, the activity of FGF21 analogs exhibit short half-lives in blood circulation and are unsuitable for current treatment. …

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Bioacoustics And Behavior Of American Crocodiles In Belize, Miriam Boucher Jan 2017

Bioacoustics And Behavior Of American Crocodiles In Belize, Miriam Boucher

Graduate Theses, Dissertations, and Problem Reports (ETD)

American Crocodiles (Crocodylus acutus) are large-bodied crocodylians broadly distributed throughout coastal and lowland wetlands in the Americas. Despite their being large, charismatic megafauna, the study of American Crocodile ecology is still lacking in many areas. As such we assessed the behavior and bioacoustics of American Crocodiles in Belize to address the paucity of data regarding these two facets of American Crocodile ecology. We conducted behavioral observations from three sites in the coastal zone of Belize. We categorized American Crocodile behavior and recorded activity duration for observed behaviors. In conjunction with behavioral data, we also assessed study sites to quantify the …

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Carbon Dioxide Capture Using Amine Functionalized Carbonaceous Materials From Bio-Refinery Waste Streams, Emmanuel Atta-Obeng Jan 2017

Carbon Dioxide Capture Using Amine Functionalized Carbonaceous Materials From Bio-Refinery Waste Streams, Emmanuel Atta-Obeng

Graduate Theses, Dissertations, and Problem Reports (ETD)

One of the greatest challenges in the world today is to replace exhaustible fossil fuel resources with renewable alternatives that will satisfy the increasing worldwide energy and chemical demands in a sustainable way. Using renewable precursors has a low impact on environmental health since it reduces pollution, greenhouse gas emissions and limits global warming and problems associated with waste management. Lignocellulosic biomass is the most available feedstock for developing sustainable chemicals and energy. A major challenge to the efficiency of the forest biorefinery industry is lack of suitable technologies for the conversion of its waste or by-products (lignin and hemicelluloses) …

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Interactions Between Euwallacea Ambrosia Beetles, Their Fungal Symbionts And The Native Trees They Attack In The Eastern United States, Matthew C. Berger Jan 2017

Interactions Between Euwallacea Ambrosia Beetles, Their Fungal Symbionts And The Native Trees They Attack In The Eastern United States, Matthew C. Berger

Graduate Theses, Dissertations, and Problem Reports (ETD)

In a globalized world, wood products are constantly being shipped from one location to another, along with tiny hitchhikers in the form of insects and microorganisms. Euwallacea validus is a fungus-farming ambrosia beetle native to East Asia that likely made its way to the United States in wood packaging materials in the latter half of the twentieth century. E. validus cultivates two fungal symbionts in the U.S., an unnamed Fusarium sp. (AF-4) and Raffaelea subfusca. Fusarium symbionts of Euwallacea ambrosia beetles as well as Raffaelea symbionts of closely related ambrosia beetles have incited widespread disease on more than one-hundred hosts …

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Thermal Processes Associated With Pelleting And Feed Phosphate Variation In Diets On Feed Manufacture And Broiler Performance, Ariel N. Bergeron Jan 2017

Thermal Processes Associated With Pelleting And Feed Phosphate Variation In Diets On Feed Manufacture And Broiler Performance, Ariel N. Bergeron

Graduate Theses, Dissertations, and Problem Reports (ETD)

The objectives of the first study were to assess performance and digestible amino acid concentration of diets fed to broilers that differed in diet formulation and degree of thermal processing. Basal diets were corn, soybean meal, and DDGS based and formulations were balanced to be similar in calculated energy and digestible amino acids. Treatments were arranged in a 3 x 3 factorial that varied in diet formulation (Basal, Basal + Meat and Bone Meal (MBM), and Basal + Bakery Byproduct (BBP)) and degree of thermal processing (Unprocessed Mash, Pellet, Double Pellet). Pelleted treatments were steam conditioned and extruded through a …

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Professional Speech And The First Amendment, Rodney A. Smolla Sep 2016

Professional Speech And The First Amendment, Rodney A. Smolla

West Virginia Law Review

No abstract provided.

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Legislation's Culture, Richard K. Neumann Jr. Sep 2016

Legislation's Culture, Richard K. Neumann Jr.

West Virginia Law Review

No abstract provided.

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Managing For Pasture Value, Edward Barrow Rayburn May 2016

Managing For Pasture Value, Edward Barrow Rayburn

Faculty & Staff Scholarship

WVU-Extension fact sheet

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Prove Yourselves: Oliver Wendell Holmes And The Obsessions Of Manliness, John M. Kang Apr 2016

Prove Yourselves: Oliver Wendell Holmes And The Obsessions Of Manliness, John M. Kang

West Virginia Law Review

No abstract provided.

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Despite What You've Been Sold - Unwrapping The Falsities Surounding Food Labels, Camille Currey Apr 2016

Despite What You've Been Sold - Unwrapping The Falsities Surounding Food Labels, Camille Currey

West Virginia Law Review

No abstract provided.

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The Aftermath Of Alt V. Epa: Unresolved Tensions In Poultry Farm Pollution Control, Alison Peck Apr 2016

The Aftermath Of Alt V. Epa: Unresolved Tensions In Poultry Farm Pollution Control, Alison Peck

West Virginia Law Review

No abstract provided.

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Pasture And Grazing Management, Edward Barrow Rayburn Apr 2016

Pasture And Grazing Management, Edward Barrow Rayburn

Faculty & Staff Scholarship

WVU-Extension fact sheet

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Nonprofit Management, Roger A. Lohmann Jan 2016

Nonprofit Management, Roger A. Lohmann

Faculty & Staff Scholarship

Nonprofit management has emerged as an important adjunct and/or subfield of public administration, largely due to the increasing use of contracted services by public agencies. In the course of this development, the meaning of nonprofit for public administration has been transformed. This article was first prepared for a PAR-affiliated website and a somewhat different version was subsequently published as a book chapter in the source cited below.

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Nanometric Resolution Magnetic Resonance Imaging Methods For Mapping Functional Activity In Neuronal Networks, Albert Boretti, Stefania Castelletto Jan 2016

Nanometric Resolution Magnetic Resonance Imaging Methods For Mapping Functional Activity In Neuronal Networks, Albert Boretti, Stefania Castelletto

Faculty & Staff Scholarship

This contribution highlights and compares some recent achievements in the use of k-space and real space imaging(scanning probe and wide-filed microscope techniques), when applied to a luminescent color center in diamond, known as nitrogen vacancy (NV) center. These techniques combined with the optically detected magnetic resonance of NV, provide a unique platform to achieve nanometric magnetic resonance imaging (MRI) resolution of nearby nuclear spins (known as nanoMRI), and nanometric NV real space localization. Atomic size optically detectable spin probe. High magnetic field sensitivity and nanometric resolution. Non-invasive mapping of functional activity in neuronal networks.

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Assessing The Association Of Mitochondrial Genetic Variation With Primary Open-Angle Glaucoma Using Gene-Set Analyses, Anthony P. Khawaja, Jessica N. Cooke Bailey, Jae Hee Kang, R. Rand Allingham, Michael A. Hauser, Murray Brilliant, Donald L. Budenz, William G. Christen, John Fingert, Douglas Gaasterland, Terry Gaasterland, Peter Kraft, Richard K. Lee, Paul R. Lichter, Yutao Liu, Felipe Medeiros, Syoko E. Moroi, Julia E. Richards, Tony Realini, Robert Ritch, Joel S. Schuman, William K. Scott, Kuldev Singh, Arthur J. Sit, Douglas Vollrath, Gadi Wollstein, Donald J. Zack, Kang Zhang, Margaret Pericak-Vance, Robert N. Weinreb, Jonathan L. Haines, Louis R. Pasquale, Janey L. Wiggs Jan 2016

Assessing The Association Of Mitochondrial Genetic Variation With Primary Open-Angle Glaucoma Using Gene-Set Analyses, Anthony P. Khawaja, Jessica N. Cooke Bailey, Jae Hee Kang, R. Rand Allingham, Michael A. Hauser, Murray Brilliant, Donald L. Budenz, William G. Christen, John Fingert, Douglas Gaasterland, Terry Gaasterland, Peter Kraft, Richard K. Lee, Paul R. Lichter, Yutao Liu, Felipe Medeiros, Syoko E. Moroi, Julia E. Richards, Tony Realini, Robert Ritch, Joel S. Schuman, William K. Scott, Kuldev Singh, Arthur J. Sit, Douglas Vollrath, Gadi Wollstein, Donald J. Zack, Kang Zhang, Margaret Pericak-Vance, Robert N. Weinreb, Jonathan L. Haines, Louis R. Pasquale, Janey L. Wiggs

Faculty & Staff Scholarship

PURPOSE. Recent studies indicate that mitochondrial proteins may contribute to the pathogenesis of primary open-angle glaucoma (POAG). In this study, we examined the association between POAG and common variations in gene-encoding mitochondrial proteins. METHODS. We examined genetic data from 3430 POAG cases and 3108 controls derived from the combination of the GLAUGEN and NEIGHBOR studies. We constructed biological-system coherent mitochondrial nuclear-encoded protein gene-sets by intersecting the MitoCarta database with the Kyoto Encyclopedia of Genes and Genomes (KEGG) database. We examined the mitochondrial gene-sets for association with POAG and with normal-tension glaucoma (NTG) and high-tension glaucoma (HTG) subsets using Pathway Analysis …

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Induction Of Cancer-Associated Fibroblast-Like Cells By Carbon Nanotubes Dictates Its Tumorigenicity, Sudjit Luanpitpong, Liying Wang, Vincent Castranova, Cerasela Zoica Dinu, Surapol Issaragrisil, Yi Charlie Yen, Yon Rojanasakul Jan 2016

Induction Of Cancer-Associated Fibroblast-Like Cells By Carbon Nanotubes Dictates Its Tumorigenicity, Sudjit Luanpitpong, Liying Wang, Vincent Castranova, Cerasela Zoica Dinu, Surapol Issaragrisil, Yi Charlie Yen, Yon Rojanasakul

Faculty & Staff Scholarship

Tumor microenvironment has been recognized as a key determinant of tumor formation and metastasis, but how tumor microenvironment is affected by nanomaterials is essentially unknown. Here, we investigated whether carbon nanotubes (CNTs), a widely used nanomaterial with known carcinogenic potential, can affect cancer-associated fibroblasts (CAFs), which are a key component of tumor microenvironment that provides necessary support for tumor growth. We show for the first time that single-walled CNT and to a lesser extent multi-walled and its COOH-functionalized form induced CAF-like cells, which are non-tumorigenic in animals, but promote tumor growth of human lung carcinoma and CNT-transformed lung epithelial cells. …

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Embryonic Markers Of Cone Differentiation, Helen M. Rodgers, Marycharmain Belcastro, Maxim Sokolov, Peter H. Mathers Jan 2016

Embryonic Markers Of Cone Differentiation, Helen M. Rodgers, Marycharmain Belcastro, Maxim Sokolov, Peter H. Mathers

Faculty & Staff Scholarship

Purpose: Photoreceptor cells are born in two distinct phases of vertebrate retinogenesis. In the mouse retina, cones are born primarily during embryogenesis, while rod formation occurs later in embryogenesis and early postnatal ages. Despite this dichotomy in photoreceptor birthdates, the visual pigments and phototransduction machinery are not reactive to visual stimulus in either type of photoreceptor cell until the second postnatal week. Several markers of early cone formation have been identified, including Otx2, Crx, Blimp1, NeuroD, Trβ2, Rorβ, and Rxrγ, and all are thought to be involved in cellular determination. However, little is known about the expression of proteins involved …

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Dual-Seq Transcriptomics Reveals The Battle For Iron During Pseudomonas Aeruginosa Acute Murine Pneumonia, F Heath Damron, Amanda G. Oglesby-Sherrouse, Angela Wilks, Mariette Barbier Jan 2016

Dual-Seq Transcriptomics Reveals The Battle For Iron During Pseudomonas Aeruginosa Acute Murine Pneumonia, F Heath Damron, Amanda G. Oglesby-Sherrouse, Angela Wilks, Mariette Barbier

Faculty & Staff Scholarship

Determining bacterial gene expression during infection is fundamental to understand pathogenesis. In this study, we used dual RNA-seq to simultaneously measure P. aeruginosa and the murine host’s gene expression and response to respiratory infection. Bacterial genes encoding products involved in metabolism and virulence were differentially expressed during infection and the type III and VI secretion systems were highly expressed in vivo. Strikingly, heme acquisition, ferric-enterobactin transport, and pyoverdine biosynthesis genes were found to be significantly up-regulated during infection. In the mouse, we profiled the acute immune response to P. aeruginosa and identified the pro-inflammatory cytokines involved in acute response …

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Oral Perfluorooctane Sulfonate (Pfos) Lessens Tumor Development In The Apcmin Mouse Model Of Spontaneous Familial Adenomatous Polyposis, Jeffrey Wimsatt, Meghan Villers, Laurel Thomas, Stacey Kamarec, Caitlin Montgomery, Leo W. Y. Yeung, Yanqing Hu, Kim Innes Jan 2016

Oral Perfluorooctane Sulfonate (Pfos) Lessens Tumor Development In The Apcmin Mouse Model Of Spontaneous Familial Adenomatous Polyposis, Jeffrey Wimsatt, Meghan Villers, Laurel Thomas, Stacey Kamarec, Caitlin Montgomery, Leo W. Y. Yeung, Yanqing Hu, Kim Innes

Faculty & Staff Scholarship

Colorectal cancer is the second most common cause of cancer deaths for both men and women, and the third most common cause of cancer in the U.S. Toxicity of current chemotherapeutic agents for colorectal cancer, and emergence of drug resistance underscore the need to develop new, potentially less toxic alternatives. Our recent cross-sectional study in a large Appalachian population, showed a strong, inverse, dose–response association of serum perfluorooctane sulfonate (PFOS) levels to prevalent colorectal cancer, suggesting PFOS may have therapeutic potential in the prevention and/or treatment of colorectal cancer. In these preliminary studies using a mouse model of familial colorectal …

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A Rapid Physical Habitat Assessment Of Wadeable Streams For Mixed-Land-Use Watersheds, Lynne Hooper, Jason A. Hubbart Jan 2016

A Rapid Physical Habitat Assessment Of Wadeable Streams For Mixed-Land-Use Watersheds, Lynne Hooper, Jason A. Hubbart

Faculty & Staff Scholarship

Mitigating stream and river impairment is complex, particularly in mixed-land-use watersheds given the likelihood of integrated responses of stream restoration to coupled and ongoing terrestrial ecosystem disturbance and the need for periodic reassessment and maintenance. Traditional biological sampling (e.g., macroinvertebrate sampling or other biological indices) alone seldom identifies the cause of biological community impairment and large fiscal investments are often made with no apparent improvement to aquatic ecosystem health. A stream physical habitat assessment (PHA) can yield information that, when paired with land-use data may reveal causal patterns in aquatic physical habitat degradation and help to identify sites for rehabilitation …

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Genome-Wide Association Study For Identifying Loci That Affect Fillet Yield, Carcass, And Body Weight Traits In Rainbow Trout (Oncorhynchus Mykiss), Dianelys Gonzalez-Pena, Guangtu Gao, Matthew Baranski, Thomas Moen, Beth M. Cleveland, P. Brett Kenney, Roger L. Vallejo, Yniv Palti, Timothy D. Leeds Jan 2016

Genome-Wide Association Study For Identifying Loci That Affect Fillet Yield, Carcass, And Body Weight Traits In Rainbow Trout (Oncorhynchus Mykiss), Dianelys Gonzalez-Pena, Guangtu Gao, Matthew Baranski, Thomas Moen, Beth M. Cleveland, P. Brett Kenney, Roger L. Vallejo, Yniv Palti, Timothy D. Leeds

Faculty & Staff Scholarship

Fillet yield (FY, %) is an economically-important trait in rainbow trout aquaculture that affects production efficiency. Despite that, FY has received little attention in breeding programs because it is difficult to measure on a large number of fish and cannot be directly measured on breeding candidates. The recent development of a high-density SNP array for rainbow trout has provided the needed tool for studying the underlying genetic architecture of this trait. A genome-wide association study (GWAS) was conducted for FY, body weight at 10 (BW10) and 13 (BW13) months post-hatching, head-off carcass weight (CAR), and fillet weight (FW) in a …

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