Digital Commons Network^™

Ontogenetic Oxycodone Exposure Affects Early Life Communicative Behaviors, Sensorimotor Reflexes, And Weight Trajectory In Mice, Elena Minakova, Simona Sarafinovska, Marwa O Mikati, Kia M Barclay, Katherine B Mccullough, Joseph D Dougherty, Ream Al-Hasani, Susan E Maloney

Open Access Publications

Nationwide, opioid misuse among pregnant women has risen four-fold from 1999 to 2014, with commensurate increase in neonates hospitalized for neonatal abstinence syndrome (NAS). NAS occurs when a fetus exposed to opioids

Srf Is Required For Maintenance Of Astrocytes In Non-Reactive State In The Mammalian Brain, Monika Jain, Soumen Das, Paul P Y Lu, Garima Virmani, Sumitha Soman, Surya Chandra Rao Thumu, David H Gutmann, Narendrakumar Ramanan

Open Access Publications

Astrocytes play several critical roles in the normal functioning of the mammalian brain, including ion homeostasis, synapse formation, and synaptic plasticity. Following injury and infection or in the setting of neurodegeneration, astrocytes become hypertrophic and reactive, a process termed astrogliosis. Although acute reactive gliosis is beneficial in limiting further tissue damage, chronic gliosis becomes detrimental for neuronal recovery and regeneration. Several extracellular factors have been identified that generate reactive astrocytes; however, very little is known about the cell-autonomous transcriptional mechanisms that regulate the maintenance of astrocytes in the normal non-reactive state. Here, we show that conditional deletion of the stimulus-dependent …

Brain Volumetric Deficits In Mapt Mutation Carriers: A Multisite Study, Stephanie A. Chu, Nupur Ghoshal, Et Al

Open Access Publications

No abstract provided.

Defining Intermediates And Redundancies In Coenzyme Q Precursor Biosynthesis, Kyle P. Robinson, Adam Jochem, Sheila E. Johnson, Thiruchelvi R. Reddy, Jason D. Russell, Joshua J. Coon, David J. Pagliarini

Open Access Publications

No abstract provided.

The Position Of Single-Base Deletions In The Vntr Sequence Of The Carboxyl Ester Lipase (Cel) Gene Determines Proteotoxicity, Anny Gravdal, Xunjun Xiao, Miriam Cnop, Khadija El Jellas, Stefan Johansson, Pål R. Njølstad, Mark E. Lowe, Bente B. Johansson, Anders Molven, Karianne Fjeld

Open Access Publications

No abstract provided.

High-Fat Diet Activates Liver Ipla2Γ Generating Eicosanoids That Mediate Metabolic Stress, Sung Ho Moon, Beverly Gibson Dilthey, Xinping Liu, Shaoping Guan, Harold F. Sims, Richard W. Gross

Open Access Publications

No abstract provided.

4Β-Hydroxycholesterol Is A Prolipogenic Factor That Promotes Srebp1c Expression And Activity Through The Liver X Receptor, Ofer Moldavski, Peter-James H. Zushin, Charles A. Berdan, Robert J. Van Eijkeren, Xuntian Jiang, Mingxing Qian, Daniel S. Ory, Douglas F. Covey, Daniel K. Nomura, Andreas Stahl, Ethan J. Weiss, Roberto Zoncu

Open Access Publications

No abstract provided.

Generation Of A Gene-Corrected Human Isogenic Ipsc Line From An Alzheimer’S Disease Ipsc Line Carrying The London Mutation In App (V717i), Damian Hernandez, Stephanie Morgan Schlicht, Maciej Daniszewski, Celeste M. Karch, Dominantly Inherited Network (Dian), Alison M. Goate, Alice Pebay

Open Access Publications

No abstract provided.

Familial Lipomas Without Classic Neurofibromatosis-1 Caused By A Missense Germline Nf1 Mutation, Erika Ramirez, Stephanie M. Morris, Tychele N. Turner, David H. Gutmann

Open Access Publications

No abstract provided.

Cytokine Profiling In Plasma From Patients With Brain Tumors Versus Healthy Individuals Using 2 Different Multiplex Immunoassay Platforms, Diane Elizabeth Bender, Maximilian O. Schaettler, Kathleen C.F. Sheehan, Tanner M. Johanns, Gavin P. Dunn

Open Access Publications

No abstract provided.

Resting-State Functional Connectivity Disruption As A Pathological Biomarker In Autosomal Dominant Alzheimer Disease, Robert X. Smith, Jeremy F. Strain, Aaron Tanenbaum, Anne M. Fagan, Jason Hassenstab, Eric Mcdade, Suzanne E. Schindler, Brian A. Gordon, Chengjie Xiong, Celeste Karch, David M. Holtzman, Daniel S. Marcus, John C. Morris, Tammie L.S. Benzinger, Randall J. Bateman, Beau M. Ances, Et Al.

Open Access Publications

No abstract provided.

Cisplatin +/− Rucaparib After Preoperative Chemotherapy In Patients With Triple-Negative Or Brca Mutated Breast Cancer, Maitri Kalra, Yang Tong, David R. Jones, Tom Walsh, Michael A. Danso, Cynthia X. Ma, Paula Silverman, Mary-Claire King, Sunil S. Badve, Susan M. Perkins, Kathy D. Miller

Open Access Publications

No abstract provided.

Dnmt3a Deficiency In The Skin Causes Focal, Canonical Dna Hypomethylation And A Cellular Proliferation Phenotype, David Y. Chen, Ian M. Ferguson, Krista A. Braun, Leslie A. Sutton, Nichole M. Helton, Sai Mukund Ramakrishnan, Amanda M. Smith, Christopher A. Miller, Timothy J. Ley

Open Access Publications

No abstract provided.

Single-Cell Analyses Of Crohn’S Disease Tissues Reveal Intestinal Intraepithelial T Cells Heterogeneity And Altered Subset Distributions, Natalia Jaeger, Ramya Gamini, Marina Cella, Jorge L. Schettini, Mattia Bugatti, Shanrong Zhao, Charles V. Rosadini, Ekaterina Esaulova, Blanda Di Luccia, Baylee Kinnett, William Vermi, Maxim N. Artyomov, Thomas A. Wynn, Ramnik J. Xavier, Scott A. Jelinsky, Marco Colonna

Open Access Publications

No abstract provided.

Monocyte Progenitors Give Rise To Multinucleated Giant Cells, Anne Kathrin Lösslein, Micah D. Dunlap, Shabaana A. Khader, Et Al.

Open Access Publications

No abstract provided.

Old Vaccines For New Infections: Exploiting Innate Immunity To Control Covid-19 And Prevent Future Pandemics, Konstantin Chumakov, Michael S. Avidan, Christine S. Benn, Stefano M. Bertozzi, Lawrence Blatt, Angela Y. Chang, Dean T. Jamison, Shabaana A. Khader, Shyam Kottilil, Mihai G. Netea, Annie Sparrow, Robert C. Gallo

Open Access Publications

No abstract provided.

The Sars-Cov-2 Nucleocapsid Protein Is Dynamic, Disordered, And Phase Separates With Rna, Jasmine Cubuk, Jhullian J. Alston, J. Jeremías Incicco, Sukrit Singh, Melissa D. Stuchell-Brereton, Michael D. Ward, Maxwell I. Zimmerman, Neha Vithani, Daniel Griffith, Jason A. Wagoner, Gregory R. Bowman, Kathleen B. Hall, Andrea Soranno, Alex S. Holehouse

Open Access Publications

No abstract provided.

Chromosome Xq23 Is Associated With Lower Atherogenic Lipid Concentrations And Favorable Cardiometabolic Indices, Pradeep Natarajan, Lucinda Antonacci-Fulton, Susan K. Dutcher, D.C. Rao, C. Charles Gu, Karen Schwander, Yun Ju Sung, Et Al.

Open Access Publications

No abstract provided.

Pushing The Needle Of Entrepreneurship And Innovation: Where Do Plastic And Reconstructive Surgeons Stand?, Sumun Khetpal, Alvaro Reátegui, Joseph Lopez, Justin M. Sacks, Adnan Prsic

Open Access Publications

No abstract provided.

Role Of Sirt1 In Isoflurane Conditioning-Induced Neurovascular Protection Against Delayed Cerebral Ischemia Secondary To Subarachnoid Hemorrhage, Meizi Liu, Keshav Jayaraman, Tusar Giri, Gregory J Zipfel, Umeshkumar Athiraman

Open Access Publications

We recently reported that isoflurane conditioning provided multifaceted protection against subarachnoid hemorrhage (SAH)-induced delayed cerebral ischemia (DCI), and this protection was through the upregulation of endothelial nitric oxide synthase (eNOS). SIRT1, an NAD-dependent deacetylase, was shown to be one of the critical regulators of eNOS. The aim of our current study is to examine the role of SIRT1 in isoflurane conditioning-induced neurovascular protection against SAH-induced DCI. Mice were divided into four groups: sham, SAH, or SAH with isoflurane conditioning (with and without EX-527). Experimental SAH via endovascular perforation was performed. Anesthetic conditioning was performed with isoflurane 2% for 1 h, …

Genetics Of Substance Use Disorders: A Review, Joseph D Deak, Emma C Johnson

Open Access Publications

Substance use disorders (SUDs) are prevalent and result in an array of negative consequences. They are influenced by genetic factors (h2 = ~50%). Recent years have brought substantial progress in our understanding of the genetic etiology of SUDs and related traits. The present review covers the current state of the field for SUD genetics, including the epidemiology and genetic epidemiology of SUDs, findings from the first-generation of SUD genome-wide association studies (GWAS), cautions about translating GWAS findings to clinical settings, and suggested prioritizations for the next wave of SUD genetics efforts. Recent advances in SUD genetics have been facilitated by …

Association Of Circulating Sex Hormones With Inflammation And Disease Severity In Patients With Covid-19, Sandeep Dhindsa, Nan Zhang, Michael J. Mcphaul, Zengru Wu, Amit K. Ghoshal, Emma C. Erlich, Kartik Mani, Gwendalyn J. Randolph, John R. Edwards, Philip A. Mudd, Abhinav Diwan

Open Access Publications

Importance: Male sex is a risk factor for developing severe COVID-19 illness. It is not known whether sex hormones contribute to this predisposition.

Objective: To investigate the association of concentrations of serum testosterone, estradiol, and insulinlike growth factor 1 (IGF-1, concentrations of which are regulated by sex hormone signaling) with COVID-19 severity.

Design, Setting, and Participants: This prospective cohort study was conducted using serum samples collected from consecutive patients who presented from March through May 2020 to the Barnes Jewish Hospital in St Louis, Missouri, with COVID-19 (diagnosed using nasopharyngeal swabs).

Exposures: Testosterone, estradiol, and IGF-1 concentrations were measured at …

Procaspase-Activating Compound-1 Synergizes With Trail To Induce Apoptosis In Established Granulosa Cell Tumor Cell Line (Kgn) And Explanted Patient Granulosa Cell Tumor Cells In Vitro, Powel Crosley, Anniina Farkkila, Adrianne L Jenner, Chloé Burlot, Olivia Cardinal, Kyle G Potts, Kate Agopsowicz, Marjut Pihlajoki, Markku Heikinheimo, Morgan Craig, Yangxin Fu, Mary M Hitt

Open Access Publications

Granulosa cell tumors (GCT) constitute only ~5% of ovarian neoplasms yet have significant consequences, as up to 80% of women with recurrent GCT will die of the disease. This study investigated the effectiveness of procaspase-activating compound 1 (PAC-1), an activator of procaspase-3, in treating adult GCT (AGCT) in combination with selected apoptosis-inducing agents. Sensitivity of the AGCT cell line KGN to these drugs, alone or in combination with PAC-1, was tested using a viability assay. Our results show a wide range in cytotoxic activity among the agents tested. Synergy with PAC-1 was most pronounced, both empirically and by mathematical modelling, …

Analysis Of Delayed Surgical Treatment And Oncologic Outcomes In Clinical Stage I Non-Small Cell Lung Cancer, Brendan T. Heiden, Daniel B. Eaton, Kathryn E. Engelhardt, Su-Hsin Chang, Yan Yan, Mayank R. Patel, Daniel Kreisel, Ruben G. Nava, Bryan F. Meyers, Benjamin D. Kozower, Varun Puri

Open Access Publications

Importance: The association between delayed surgical treatment and oncologic outcomes in patients with non-small cell lung cancer (NSCLC) is poorly understood given that prior studies have used imprecise definitions for the date of cancer diagnosis.

Objective: To use a uniform method to quantify surgical treatment delay and to examine its association with several oncologic outcomes.

Design, Setting, and Participants: This retrospective cohort study was conducted using a novel data set from the Veterans Health Administration (VHA) system. Included patients had clinical stage I NSCLC and were undergoing resection from 2006 to 2016 within the VHA system. Time to surgical treatment …

Current And Prospective Methods For Assessing Anti-Tumor Immunity In Colorectal Cancer, Yulia I Nussbaum, Yariswamy Manjunath, Kanve N Suvilesh, Wesley C Warren, Chi-Ren Shyu, Jussuf T Kaifi, Matthew A Ciorba, Jonathan B Mitchem

Open Access Publications

Colorectal cancer (CRC) remains one of the deadliest malignancies worldwide despite recent progress in treatment strategies. Though immune checkpoint inhibition has proven effective for a number of other tumors, it offers benefits in only a small group of CRC patients with high microsatellite instability. In general, heterogenous cell groups in the tumor microenvironment are considered as the major barrier for unveiling the causes of low immune response. Therefore, deconvolution of cellular components in highly heterogeneous microenvironments is crucial for understanding the immune contexture of cancer. In this review, we assimilate current knowledge and recent studies examining anti-tumor immunity in CRC. …

Braf-Mek Inhibitors As Steroid-Sparing Bridge Prior To Checkpoint Blockade Therapy In Symptomatic Intracranial Melanoma, Karam Khaddour, Tanner M. Johanns, George Ansstas

Open Access Publications

The introduction of immune checkpoint blockade (ICB) and BRAF-MEK inhibitors has substantially improved outcomes in patients with metastatic melanoma. However, several challenging factors may hinder the efficacy of ICB in patients with symptomatic intracranial metastatic melanoma who are immunosuppressed due to the use of steroids prior to the administration of ICB. This has resulted in the exclusion of patients treated with high dose steroid at baseline from the majority of ICB clinical trials. In addition, despite the high efficacy of BRAF-MEK inhibitors in

Diagnostic Accuracy Of Health Care Administrative Diagnosis Codes To Identify Nontuberculous Mycobacteria Disease: A Systematic Review, Carlos Mejia-Chew, Lauren Yaeger, Kevin Montes, Thomas C. Bailey, Margaret A. Olsen

Open Access Publications

Background: Health care administrative database research frequently uses standard medical codes to identify diagnoses or procedures. The aim of this review was to establish the diagnostic accuracy of codes used in administrative data research to identify nontuberculous mycobacterial (NTM) disease, including lung disease (NTMLD).

Methods: We searched Ovid Medline, Embase, Scopus, Cochrane Database of Systematic Reviews, Cochrane Central Register of Controlled Trials, and ClinicalTrials.gov from inception to April 2019. We included studies assessing the diagnostic accuracy of

Results: We identified 5549 unique citations. Of the 96 full-text articles reviewed, 7 eligible studies of moderate quality (3730 participants) were included in …

Longitudinal Growth Differentiation Factor 15 (Gdf15) And Long-Term Intraocular Pressure Fluctuation In Glaucoma: A Pilot Study, Jonathan B. Lin, Arsham Sheybani, Andrea Santeford, Rajendra S. Apte

Open Access Publications

No abstract provided.

Functional Interaction Between Wnt And Bmp Signaling In Periosteal Bone Growth, Deye Song, Guangxu He, Yu Shi, Jiangdong Ni, Fanxin Long

Open Access Publications

No abstract provided.

Multitarget Stool Rna Test For Noninvasive Detection Of Colorectal Neoplasia In A Multicenter, Prospective, And Retrospective Cohort, Erica K. Barnell, Yiming Kang, Andrew R. Barnell, Kimberly R. Kruse, Jared Fiske, Zachary R. Pittz, Adnan R. Khan, Thomas A. Huebner, Faith L. Holmes, Malachi Griffith, Obi L. Griffith, Aadel A. Chaudhuri, Elizabeth M. Wurtzler

Open Access Publications

No abstract provided.