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Development Of Analytical Methods For Analysis Of Glycosaminoglycans And Their Mimetics In Human Plasma, Rawan M. Fayyad

Theses and Dissertations

Glycosaminoglycans (GAGs) are linear, polydisperse polysaccharides consisting of alternating amino sugars and either a uronic acid or galactose residue. They are ubiquitous components of the extracellular matrix and are present on cell surfaces and in basement membranes. GAGs are highly negatively charged as a result of many sulfate and carboxylic acid groups, and are extremely heterogeneous in both their structure and in their physicochemical properties. This complexity in structure is attributed to the alterations in residue types, glycosidic bond linkage, sulfation levels, sulfation positions, and chain lengths during biosynthesis. GAGs interact with a wide number of proteins and modulate various …

The Role Of Autophagy And Senescence In The Responses Of Non-Small Cell Lung Cancer Cells To Chemotherapy And Radiation, Nipa H. Patel

Theses and Dissertations

Cancer-associated deaths account for the second-highest mortality rates in the United States. Primary modalities of treatment often include surgery, radiation, and chemotherapy, and may also incorporate targeted therapy and immunotherapy. However, resistance to these treatments remains high, resulting in disease reoccurrence and poor survival rates. While apoptosis or cell death of tumor cells is the ideal outcome for anti-cancer therapy, this is often not the case, and in fact cancer cells may upregulate several pathways, such as autophagy and senescence, as a means to undergo alternative cell fate and evade apoptotic cell death. An essential tumor suppressor gene, TP53, …

Bisphosohoglycertae Mutase: A Potential Target For Sickle Cell Disease, Anfal S. Aljahdali

Theses and Dissertations

Bisphosphoglycerate mutase (BPGM) is a part of the erythrocyte glycolysis system. Specifically, it is a central enzyme in the Rapoport-Leubering pathway, a side glycolytic pathway involved in the regulation of the concentration of the natural allosteric effector of hemoglobin (Hb), 2,3-bisphosphoglycerate (2,3-BPG). BPGM catalyses the synthesis and hydrolysis of 2,3-BPG through its synthase and phosphatase activities. The synthase activity is the main role of BPGM, while the phosphatase activity is low and is activated by the physiological effector, 2-phosphoglycolate (2-PG) with the latter mechanism poorly understood.

BPGM activity and 2,3-BPG levels in red blood cells (RBCs) have a significant role …

Learning-Based Predictive Control Approach For Real-Time Management Of Cyber-Physical Systems, Roja Eini

Theses and Dissertations

Cyber-physical systems (CPSs) are composed of heterogeneous, and networked hardware and software components tightly integrated with physical elements [72]. Large-scale CPSs are composed of complex components, subject to uncertainties [89], as though their design and development is a challenging task. Achieving reliability and real-time adaptation to changing environments are some of the challenges involved in large-scale CPSs development [51]. Addressing these challenges requires deep insights into control theory and machine learning. This research presents a learning-based control approach for CPSs management, considering their requirements, specifications, and constraints. Model-based control approaches, such as model predictive control (MPC), are proven to be …

Glycosaminoglycan Modulation Of Key Proteins In Alzheimer's Disease, Tamim Chiba

Theses and Dissertations

Glycosaminoglycans (GAGs) are linear polysaccharides whose disaccharide building blocks consist of an amino sugar (D-glucosamine) and either uronic acid (D-glucuronic acid or L-iduronic acid) or galactose. Nearly all GAGs (with the exception of hyaluronic acid) are covalently attached to proteins, forming proteoglycans, and are ubiquitous in nature. The monomeric units, chain length, degree and pattern of sulfation, and epimerization are important properties that determine the influence of these molecules. Through Coulombic forces, hydrogen bonds and hydrophobic interactions, they regulate many physiological and pathophysiological processes such as blood coagulation, cell growth and differentiation, inflammatory processes, host defense and viral infection mechanisms. …

Regulation Of Osseous Healing By Macrophage-Derived Wnt Ligands, Jefferson Overlin Abaricia

Theses and Dissertations

Osseous healing is regulated by various classes of cells and signaling mechanisms. Of these, Wnt signaling has long been known to be critically important in directing the differentiation of mesenchymal stem cells (MSCs) into osteoblasts during bone healing. However, the source of these Wnt ligands is not known. Recently, a body of literature has arisen finding both beneficial and detrimental effects of macrophage-derived Wnt ligands on the regeneration of other tissues (liver, kidney, heart, and intestine), often by regulating inflammatory activation and stem cell behavior. As macrophages have been well-characterized as orchestrators of healing, their use of Wnt signaling to …

Intermittent Theta Burst Stimulation: Application To Spinal Cord Injury Rehabilitation And Computational Modeling, Neil Mittal

Theses and Dissertations

Loss of motor function from spinal cord injuries (SCI) results in loss of independence. Rehabilitation efforts are targeted to enhance the ability to perform activities of daily living (ADLs), but outcomes from physical therapy alone are often insufficient. Neuromodulation techniques that induce neuroplasticity may push the limits on recovery. Neuromodulation by intermittent theta burst transcranial magnetic stimulation (iTBS) induces neuroplasticity by increasing corticomotor excitability, though this has most frequently been studied with motor targets and on individuals not in need of rehabilitation. Increased corticomotor excitability is associated with motor learning. The response to iTBS, however, is highly variable and unpredictable, …

Stat5a Regulation By Serine Phosphorylation In Breast Cancer, Alicia E. Woock

Theses and Dissertations

The neuroendocrine hormone prolactin (PRL) and its cognate receptor (PRLr) have been implicated in the pathogenesis of breast cancer. PRL signaling relies on activating kinases such as the tyrosine kinase Jak2 and serine/threonine kinases ERK1/2, NEK3, PI3K, and AKT. In the canonical pathway of PRL signaling, JAK2 phosphorylates the transcription factor STAT5a at tyrosine residue 694 (pY694-STAT5a), preceding STAT5a nuclear translocation and transcriptional activity. However, STAT5a exists with functional duality as a transcription factor, having both pro-differentiative and pro-proliferative target genes. Other STAT family members (STATs 1, 3, and 6) have been shown to have transcriptional activity in the un-tyrosine-phosphorylated …

The Student Experience Of Higher Education During The Covid-19 Pandemic: How Class And Race Shape Socially Distanced Learning At A Public University, Alice M. Quach

Theses and Dissertations

While prior research has examined trends toward growing uncertainty and precarity for young adults within the economy and higher education, the COVID-19 pandemic appears to be exacerbating these existing inequalities and risks. Drawing from 30 semi-structured in-depth interviews with college students at a large Southern and urban public university, this study examines how the COVID-19 pandemic is affecting students’ learning experiences, and how those experiences are differentially shaped, in turn, by their class and race. This exploratory research illuminates our understanding of the multiple mechanisms by which the COVID-19 pandemic is shaping existing inequalities in the higher education system, mostly …

Survival, Growth, And Characterization Of Microbes In E-Liquids, Jessica Bunn

Master of Science in Forensic Science Directed Research Projects

Health risks associated with the use of e-cigarettes have become a concern in the last ten years, including the inhalation of volatile organic compounds, trace heavy metals, and cancer-causing agents. An additional, but poorly characterized health threat for e-liquids is the potential for bacterial contamination since the presence of pathogenic organisms can potentially cause disease from the inhalation of the microbes into the lungs. This work aims to investigate microbial growth and survival within the e-liquid matrix. Bacillus cereus T. strain and Escherichia coli were the chosen organisms for analysis of growth by absorbance, survival by viable plate counts, and …

Quantitative Pcr And Sanger Sequencing Of Mitochondrial Dna Recovered From Waterlogged Bone, Kailey Babcock

Master of Science in Forensic Science Directed Research Projects

In forensic contexts, samples containing heavily fragmented DNA are commonly encountered. Compromised biological samples are especially prevalent in instances where human remains have been submerged in an aqueous environment for extended periods of time. Nuclear DNA is particularly vulnerable to the prolonged exposure to heat, moisture, and bacterial degradation that are prevalent in aquatic settings. Paired with the difficulty of recovering DNA from skeletal remains, which are often the only remaining component after the soft tissues have been stripped away, mitochondrial DNA (mtDNA) analysis serves as an invaluable alternative. In this multifaceted study, mtDNA analysis was performed on waterlogged bone …

The Impact Of Latent Print Processing On Firing Pin And Breechface Impressions During Firearm Examination, Brittany B. Harrington

Master of Science in Forensic Science Directed Research Projects

Latent prints can be a valuable source of forensic evidence when solving a crime. They can verify if a person was at a specific scene, identify unknown individuals to connect them to a scene, and help to corroborate eyewitness accounts. Latent prints, however, are not always visible until they have undergone enhancement or visualization techniques. When fired cartridge cases are suspected of containing latent prints, they are brought in for latent print processing before any firearm analysis is performed. As a result, these cartridges are often coated in various residues or dyes when they arrive for firearm examination. In response, …

Powerplex® Fusion 6c System Versus Powerplex® Fusion 5c: A Comparison Of Performance Metrics, Caitlin M. Mccaughan

Master of Science in Forensic Science Directed Research Projects

Following the FBI mandated expansion of the CODIS core loci from 13 to 20, several manufacturers developed short tandem repeat (STR) typing kits in response to the new criteria. One such manufacturer was the Promega Corporation, which released the PowerPlex® Fusion 5C megaplex STR typing kit (Fusion 5C) in 2012. Currently, the Virginia Department of Forensic Science (VDFS) utilizes this amplification kit for both casework and database applications. In 2015, Promega released the PowerPlex® Fusion 6C STR typing kit (Fusion 6C), which contains three additional loci beyond those included in PowerPlex® Fusion 5C, and a sixth color channel. The power …

The Efficacy Of Recovering Latent Fingerprints From Fired Cartridge Cases By Way Of Cyanoacrylate Fuming Combined With Basic Yellow Dye Staining, Lily Mantz

Master of Science in Forensic Science Directed Research Projects

This experiment attempted to tackle the long-standing struggle to consistently recover latent fingerprints from fired cartridges. With an emphasis on realistic handling of high volume of samples combined with Cyanoacrylate fuming followed by Basic Yellow dye staining, a total of 740 cartridges were examined. These included fired and unfired brass and nickel case 9mm Luger and 40 S&W cartridges. The cartridges were divided into five different groups; each were treated differently prior to cyanoacrylate fuming and dye staining, which was conducted in the same manner for the groups. The groups included the positive and negative controls, loaded, cycled, and fired. …

Analysis Of Ethanol Aerosolized In An Electronic Cigarette By Headspace Gas Chromatography-Flame Ionization Detector (Gc-Fid) And Particle Size Separation, Erica R. Sales

Master of Science in Forensic Science Directed Research Projects

Electronic cigarettes (e-cigs) were designed as a healthier alternative nicotine delivery system, and involve the heating of e-liquids. These are composed of propylene glycol, vegetable glycerin, flavoring agents, and nicotine. Recently, ethanol has been identified as an unlabeled ingredient. The health consequences are currently unknown.

The purpose of this study is to quantify the amount of ethanol present in an aerosol cloud and to determine the size of the ethanol particles. A SubOx Mini-C e-cig was used. For ethanol quantification, the e-cig was attached to a Buchner Flask containing 150mL of deionized water, The system was attached to a vacuum …

Differentiation Between Peripheral Blood And Menstrual Blood Using Mirna Markers, Anaya Valentine

Master of Science in Forensic Science Directed Research Projects

Abstract

In forensic investigations, body fluids can provide crucial information and is helpful for corroborating the circumstance of the case. For cases of sexual assaults or homicides, being able to differentiate if the blood is peripheral blood or menstrual blood is important. Peripheral blood can be indicative of a traumatic event, while menstrual blood is of a natural cause. Currently, serology based methods are used for body fluid identification, however, their lack of sensitivity and specificity remains an issue. MicroRNAs (miRNAs) are a class of non-protein coding nucleic acids that are able to be co-extracted with DNA, and their small …

Evaluation Of Dna Extraction Efficiencies Of Promega’S Dna Iq™ Methods And Casework Extraction Kit For Low Template Samples, Emily M. Anderson

Master of Science in Forensic Science Directed Research Projects

Biological evidence from crime scene samples frequently contain low levels of DNA, such as the most predominant form of evidence, which is DNA deposited by handling objects or “touch evidence”. To maximize the DNA yield recovered from theses challenging samples, forensic laboratories must optimize the extraction methods utilized to isolate and purify DNA for downstream short tandem repeat (STR) amplifications. Currently, the Virginia Department of Forensic Science (VADFS) uses a DNA IQ™ System (DNA IQ) extraction method for isolation of DNA from most forensic samples. This extraction procedure, which combines DNA IQ™ lysis buffer and Dithiothreitol (DTT), has been validated …

Testing The Efficacy Of Surface Swab Sampling To Determine Post-Mortem Submersion Interval (Pmsi), Using The Microbiome Colonization Of Skeletal Remains Submerged In A Lotic Environment, Sarah Rose

Master of Science in Forensic Science Directed Research Projects

Estimating the post-mortem submersion interval (PMSI) can provide a valuable forensic tool for medicolegal death investigations involving victims discovered in aquatic environments. Previous studies conducted by Cartozzo et al. (2021) successfully demonstrated the use of microbial succession to create predictive models for the estimation of PMSI from submerged bone. Though effective, bone sampling requires time consuming processing techniques that result in destruction of decedent tissue. This study investigates the use of bone surface swabbing as an effective alternative method to bone sampling, with the goal of predicting PMSI using a simpler, non-invasive sampling technique. Porcine (Sus scrofa) skeletal remains (rib …

Activity Of Saccharomyces Cerevisiae By Single Entity Electrochemistry, John Lutkenhaus

Graduate Research Posters

According to the Centers of Disease Control and Prevention, antibiotics decrease in effectiveness as bacteria gain resistance for previously treatable illnesses. Currently, antibiotic susceptibility is typically carried out via the Kirby-Bauer method. Even with automation, this process requires two incubation periods so a less time-consuming technique is desirable. Single entity electrochemistry (SEE) detects changes in current when collisions of individual particles at an ultramicroelectrode (UME) are linked with an electrochemical event. Our group has obtained step-like and spike-like responses of Saccharomyces cerevisiae at the UME surface as a result of adsorption and desorption, respectively. This response is due to the …

Vasculogenic Mimicry: Role Of Melanoma Differentiation Associated Gene-9/Syntenin, Jinkal Modi, Anjan Pradhan, Luni Emdad, Swadesh Das, Paul Fisher

Graduate Research Posters

Malignant melanoma (MM) is the most aggressive skin cancer and the most frequent skin disorder in Caucasians. MM is associated with aggressive and progressive disease states, leading to major cancer-related morbidity and mortality. Recent investigations identify a new non-angiogenesis-dependent pathway vasculogenic mimicry (VM), which is considered a cancer hallmark that can independently facilitate tumor neovascularization by the formation of fluid-conducting and vascular endothelial cells. MM cells undergoing VM can dedifferentiate into numerous cellular phenotypes and acquire endothelial-like features, resulting in the formation of the de novo matrix-rich vascular-like network, such as plasma and red blood cells. The co-generation of endothelial …

Colobinae Evolution: Using Gis To Map The Distribution Of Leaf Monkeys Across Southeast Asia Over Time, Marie Vergamini, Christina Mcgrath, Lisa M. Day

Graduate Research Posters

The Colobinae, or leaf monkeys, are distributed geographically across Africa and Asia. Colobinae are specialized arborealists and leaf eaters with sacculated stomachs, sheering teeth, reduced thumbs, and very mobile shoulders. Colobinae diverged ~10.9 million year ago (Ma) from the Cercopithecidae in Africa, and Asian colobines appear in the fossil record in the late Miocene ~8.5 Ma. However, an incomplete fossil record means little is known about the evolutionary pressures that led to Asian colobine migration and diversification. Here, we use recent fossil discoveries and geospatial information to develop hypotheses about how geographic barriers played direct roles in Asian colobine evolution. …

Attitudes And Opinions About Direct-To-Consumer Genetic Testing In Undergraduate Science Students, Morgan N. Driver, Sally I-Chun Kuo, Olivia Nayeri, Chloe J. Walker, Chelsea Derlan Willians, Tricia Smith, Amy E. Adkins, Danielle M. Dick

Graduate Research Posters

Background: There has been exponential growth in the number of direct-to-consumer genetic testing kits sold in the past decade. Consumers utilize direct-to-consumer genetic tests for a number of reasons which include learning about one’s ancestry and potential ways to manage health. Emerging adults tend to be early adopters of new technologies; however, there has been little research regarding the opinions about direct-to-consumer genetic testing in emerging adults.

Methods: Data came from a study conducted in an upper-level biology course focusing on understanding undergraduate science students’ overall experiences with receiving personalized genetic testing results from 23andMe. The present study used data …

Radiation Induces Metabolic Dysregulation In Pulmonary Fibroblasts, Josly Pierre-Louis, Margaret A. T. Freeberg, Jane K. Rebman, Thomas H. Thatcher, Patricia J. Sime

Graduate Research Posters

Rationale: Exposure of the lung to ionizing radiation, such as during radiotherapy, can result in pulmonary fibrosis (PF), which has few treatment options. PF is characterized by an accumulation of extracellular matrix proteins that form scar tissue, resulting in dyspnea, disruption of gas exchange, and even death. We and others have shown that metabolic reprogramming is a hallmark of idiopathic pulmonary fibrosis (IPF). IPF lung tissue, and lung fibroblasts treated with TGF-β, exhibit increased aerobic glycolysis with increased expression of lactate dehydrogenase A (LDHA) and excess production of lactate, leading to reduced extracellular pH that activates latent TGF-β. Here, we …

Transcriptional Repressor Protein Based Macrolide Biosensor Development With Improved Sensitivity, Jayani A. Christopher

Graduate Research Posters

Macrolide antibiotics are in high demand for clinical applications. Macrolides are biosynthesized via giant assembly line polyketide synthases (PKS) which are arranged in a modular fashion. Combinatorial biosynthetic methods have been used to produce diversified macrolides by reprograming these modules and modifying tailoring enzymes required for post synthetic modifications. However it is challenging due to the size and complexity of PKSs. To overcome this challenge, new enzymes for macrolide diversification could be obtained by directed evolution where a large number of enzyme variants need to be screened. Therefore it is important to develop high throughput screening methods to identify the …

Modeling Longitudinal Change In Cervical Length Across Pregnancy, Hope M. Wolf, Shawn J. Latendresse, Jerome F. Strauss Iii, Timothy P. York

Graduate Research Posters

Introduction: A short cervix (cervical length < 25 mm) in the mid-trimester (18 to 24 weeks) of pregnancy is a powerful predictor of spontaneous preterm delivery (gestational age at delivery < 37 weeks). Although the biological mechanisms of cervical remodeling have been the subject of extensive investigation, very little is known about the rate of change in cervical length over the course of a pregnancy, or the extent to which rapid cervical shortening increases maternal risk for spontaneous preterm delivery.

Methods: A cohort of 5,160 unique women carrying 5,971 singleton pregnancies provided two or more measurements of cervical length during pregnancy. Cervical length was measured in millimeters using a transvaginal 12-3 MHz ultrasound endocavity probe (SuperSonic Imagine). Maternal characteristics, including relevant medical history and birth outcome data, were collected for each participant. Gestational age at delivery was measured from the first day of each woman’s last menstrual period and confirmed by ultrasound. Repeated measurements of cervical length during pregnancy were modeled as a longitudinal, multilevel growth curve in MPlus. A three-level variance structure was …

The Association Of Menthol Cigarette Use With Any Mental Illness (Ami) And Serious Mental Illness (Smi) Among Us Smoekrs, Augustus White

Graduate Research Posters

ABSTRACT

Background: Mental illness affects 1-in-5 adults in the United States (US). Previously, those with mental illness have been shown to be 70% more likely to smoke cigarettes than the general population. However, little is known regarding whether the use of menthol cigarettes (one of only two available “characterizing flavors” of cigarettes) might impact the association between tobacco use and mental illness. Such information is critical in light of the disproportionately high rate of use of menthol cigarettes observed among African American smokers, as well as to inform recent legislative proposals that seek to ban the sale of menthol cigarettes. …

Influence Of Immigration Status On 10-Year Psychosocial Functioning In Individuals With Tbi, Chimdindu Ohayagha, Paul Perrin, Juan Carlos Arango-Lasprilla

Graduate Research Posters

No abstract provided.

Peptidomimetic And Non- Peptidomimetic Derivatives As Possible Sars-Cov-2 Main Protease (Mpro) Inhibitors, Mohammed A. Al Awadh, Mohini S. Ghatge Ph.D, Mona A. Al Khairi, Faik N. Musayev, Akua K. Donkor, Mohammed H. Al Mughram, Abdelsattar M. Omar Ph.D, Moustafa M. El-Araby Ph.D, Martin K. Safo Ph.D

Graduate Research Posters

To design novel inhibitors of the SARS-CoV-2 main protease (Mpro), we investigated the binding mode of the recently reported α-ketoamide inhibitors of this enzyme. Following, we utilized in-silico screening to identify 168 peptidomimetic and non-peptidomimetic compounds that are high probability Mpro binding candidates. The compounds were synthesized in 5 to 10 mg for initial screening for their potential inhibition of Mpro using Fluorescence Resonance Energy Transfer (FRET) assay. The study was conducted using the main protease, MBP-tagged (SARS-CoV-2) Assay Kit (BPS Bioscience, #79955-2), and the fluorescence due to enzymatic cleavage of substrate measured using BMG LABTECH CLARIOstar™, a fluorescent microplate …

Development Of An Online Warfarin Dosing Platform Using R Programming Language To Facilitate Healthcare Professional Duties And Limit Medication Related Errors., Monther Alsultan

Graduate Research Posters

Development of an online warfarin dosing platform using R programming language to facilitate healthcare professional duties and limit medication related errors.

Monther Alsultan, Joshua M. Morriss, Daniel Contaifer Jr, Suad Alshammari; Silas Contaifer, Rachel W Flurie, Dayanjan S. Wijesinghe

Department of Pharmacotherapy and Outcome Sciences

Objective: 1) Gain experience in developing platform agnostic, fully operational and clinically relevant web applications for effective pharmacist led patient care. 2) Create a decision- support tool using open source software to facilitate evidence-based management therapy of warfarin in clinical settings where it is available for everyone to use at anytime and anywhere.

Introduction: Healthcare …

Virginia Commonwealth University Undergraduate Bulletin [2021], Virginia Commonwealth University

VCU Bulletins

Undergraduate bulletin for Virginia Commonwealth University for the academic year 2021-2022. It includes information on academic regulations, degree requirements, course offerings, faculty, academic calendar, and tuition and expenses for undergraduate programs.