Digital Commons Network^™

Progress Towards Next Generation Modulators Of Ctbp's Oncogenic Effects, Jacqueline L. West

Theses and Dissertations

Human C-terminal binding proteins (CtBP1 and CtBP2) are transcriptional coregulators of multiple genes in the human genome, including tumor suppressor genes (e.g., Bik, PTEN, BRCA1, and E-cadherin) as well as oncogenes (e.g. MDR1 and Tiam1). Both homologues of CtBP are overexpressed in many types of cancer, including breast cancer (92%), ovarian cancer (83%), colorectal cancer (64%), hepatocellular carcinoma (60%), gastric cancer, prostate cancer, and pancreatic adenocarcinoma. Further, expression levels of CtBP correlate with worse prognostic outcomes and more aggressive tumor features because it promotes proliferation, epithelial-mesenchymal transition, and cancer stem cell self-renewal activity.

Our laboratory has identified a lead inhibitor …

The Influence Of Salinity On The Structure And Function Of Soil Prokaryotic Communities In Coastal Freshwater Wetlands, Joseph C. Morina Ii

Theses and Dissertations

Microbial communities are critical biological components of the world’s ecosystems, and their respiratory pathways are directly involved in the biogeochemical cycling of essential nutrients. As genomic technologies advance, allowing for more detailed profiling of microbial communities, efforts have successfully linked microbial community composition to ecosystem-level functions and have shown microbial communities are susceptible and resistant to disturbance events. The goal of this dissertation is to address the temporal scales in which microbial communities respond to the disturbance of salinization, and the repercussions this has on microbially-meditated carbon and nitrogen cycling. Coastal freshwater wetlands are an excellent study system to investigate …

A Gun For Every Woman, Gentë Retkoceri

Theses and Dissertations

Six centuries ago in Kosova, my home country, unmarried women known as sworn virgins, were forced to adopt a male persona and carry a gun to protect themselves. But today, guns have become tools of oppression and violence against women. And while the legal system carries penalties for domestic violence, marital rape is excluded. To shine a light on, and expose the topic of hidden violence, my thesis uses artivism to address a sensitive but provocative issue, exhibiting a large-scale sculpture of a gun, designed to provoke raw emotions; to challenge visitors to consider the terror felt by women threatened …

Rational Design Of Flexible And Stretchable Electronics Based On 3d Printing, Yuanhang Yang

Theses and Dissertations

Flexible and stretchable electronics have been considered as the key component for the next generation of flexible devices. There are many approaches to prepare the devices, such as dip coating, spin coating, Mayer bar coating, filtration and transfer, and printing, etc. The effectiveness of these methods has been proven, but some drawbacks cannot be ignored, such as lacking pattern control, labor consuming, requiring complex pretreatment, wasting conductive materials, etc.

In this investigation, we propose to adopt 3D printing technology to design flexible and stretchable electronics. The objective is to rationally design flexible and stretchable sensors, simplify the preparation process, form …

Innovative Techniques Of Neuromodulation And Neuromodeling Based On Focal Non-Invasive Transcranial Magnetic Stimulation For Neurological Disorders, Ivan C. Carmona-Tortolero

Theses and Dissertations

This dissertation aims to develop alternative technology that improves the current range of application of transcranial magnetic stimulation (TMS), on a scale that would permit defining specific non-invasive treatments for Parkinson’s disease and other neurological disorders. This is accomplished through three specific objectives. 1) The design of a neurostimulation system that increases the focality in TMS to regions of narrow target areas and variable depths in the brain cortex. 2) The assessment of the feasibility of novel high-frequency neuromodulation techniques that would allow increasing the focality in deeper areas beyond the cortical surface. 3) The development of a computational model …

Improving The Early Detection Of Cardiovascular Toxicity Secondary To Radiotherapy For Lung Cancer Via Patient-Specific Quantitative Magnetic Resonance Imaging, Alireza Omidi

Theses and Dissertations

Purpose: To assess the cardiopulmonary-induced dose variation on the left ventricle (LV) and evaluate the spatiotemporal evolution of cardiac/aortic function following radiotherapy (RT).

Methods: 8 lung cancer patients who were scheduled to receive RT were recruited for this study. Each patient underwent one 4D-CT at baseline. MRI scans including cine GRE, T1/T2, LGE, and 4D-flow were acquired at baseline, 3-months and 6-months post-RT to evaluate the cardiac/aortic function. Finally, image registration was used to assess the cardiopulmonary-induced dose variation on the LV.

Results: Mean RT-dose was minimum during inspiration and systole (at expiration). No significant differences were found in the …

Analysis Of Non-Conventional Radiological Terrorism, James N. Padgett

Theses and Dissertations

Nuclear terrorism has been a risk since the dawn of the first atomic bomb. Though state sponsored nuclear weapons development is of concern for countries, non-state sponsored terrorism with radiological material can be of even greater concern. This stems from the fact that the material is under less stringent or no safeguards and can readily change hands between different terrorist groups or innocent civilians may accidently come into contact with the material.

Within this paper an analysis of previous accidents using orphan radiological sources, malicious use of orphan radiological sources, and how these sources could be used by terrorists is …

Universal Design In Bci: Deep Learning Approaches For Adaptive Speech Brain-Computer Interfaces, Srdjan Lesaja

Theses and Dissertations

In the last two decades, there have been many breakthrough advancements in non-invasive and invasive brain-computer interface (BCI) systems. However, the majority of BCI model designs still follow a paradigm whereby neural signals are preprocessed and task-related features extracted using static, and generally customized, data-independent designs. Such BCI designs commonly optimize narrow task performance over generalizability, adaptability, and robustness, which is not well suited to meeting individual user needs. If one day BCIs are to be capable of decoding our higher-order cognitive commands and conceptual maps, their designs will need to be adaptive architectures that will evolve and grow in …

Characteristics Of Culturally Inclusive Art Education Pedagogy: A Historical Document Analysis Study, Fatemah A. Khawaji

Theses and Dissertations

The objective of this mixed methods historical document analysis is to identify the characteristics of culturally inclusive art education pedagogy. Using the theoretical lenses of intersectionality, critical theory, and socially inclusive pedagogy, this study seeks to determine the reasons for the misapplication of diversity and inclusion in art education. Qualitative and quantitative methods are applied to the historical document analysis of National Art Education Association articles in two major journals: Art Education and Studies in Art Education. The findings indicate that for the last 20 years, 20% of the articles published in Studies in Art Education and 30% of …

Targeting Bcl-2 Family Proteins In Therapy Induced Senescent Cancer Cell Models, Wade Cook

Theses and Dissertations

Non-small Cell Lung Cancer (NSCLC) originates from numerous different cell types in the lungs and is among the deadliest of cancers. Head and Neck Squamous Cell Carcinomas (HNSCC) are derived from the mucosal membranes of the oral cavity, pharynx, and larynx. Both NSCLC and HNSCC are predominately caused by tobacco smoke inhalation and as such mutations in the tumor suppressor gene TP53 are common. Since similarities exist in the root cause of NSCLC and HNSCC, they may also share similarities in treatment methods. Cisplatin is a platinum-based DNA damaging agent that has been used as a cancer chemotherapy for decades. …

Evaluating Population Genetic Structure And Potential Genomic Signals Of Natural Selection In A Migratory Songbird (Protonotaria Citrea), Tyler A. Hohenstein

Theses and Dissertations

In this study I attempted to further resolve the population genetic structure in the Prothonotary Warbler (Protonotaria citrea), and conducted an outlier SNP analysis and exploratory gene ontology analysis to investigate potential ongoing natural selection in the species. This analysis of population structure confirms previous work by DeSaix et al. (2019), where weak population structure was observed between eastern sites along the Atlantic Coastal Plain, and western sites in the Mississippi Alluvial Valley, possibly due to a genetic discontinuity across the Appalachian Mountains. I conducted two forms of outlier SNP analyses, a principal component analysis (PCA)-based approach to identify SNPs …

Development Of Novel, Miniaturized Sperm Cell Isolation Techniques For Sexual Assault Samples, Brittany Celeste Hudson

Theses and Dissertations

The ever-increasing sexual assault evidence collection kit backlog within the United States has prompted the search (and desperate need) for a more efficient, cost-effective, and rapid processing technique that can separate male and female contributions from evidentiary samples. Although backlogs have largely been tackled across the United States and many technological advancements have made the forensic DNA analysis workflow quicker, techniques for handling sexual assault samples remain time-consuming, tedious, and inefficient at separating cellular fractions from the victim and perpetrator. Thus, this research sought to identify a reproducible sperm isolation method using antibodies and/or alternative differential cell lysis techniques as …

Developmental Validation Of A Bacterial Signature-Based Identification Method Of Forensically Relevant Human Biological Samples, Denise M. Wohlfahrt

Theses and Dissertations

The confirmatory identification of body fluids is one of the most critical steps in criminal investigations, as it provides not only information on the source of the DNA, but it can corroborate evidence, aid in the reconstruction of a crime, and associate evidence with a person of interest. Current serological methods utilize biochemistry and immunology to identify biological evidence; however, these serological tests are mostly presumptive while only testing for one fluid at a time, potentially wasting precious evidence. More recently, forensic investigators have suggested the use of molecular methods such as mRNA, miRNA, or DNA methylation. While each of …

Representativeness Of Data From An Online Sample Of Individuals With Severe Alcohol Use Disorder, Michelle Eglovitch

Theses and Dissertations

Understanding the characteristics of research participants who provide DNA is crucial to ensuring sample representativeness and generalizability of findings of genetics studies of substance use disorders. Using anonymous survey data, the present study had a unique opportunity to compare participants who provided DNA (n=2,414) with those that did not provide DNA (n=1,515). The present study found numerous differences between groups across demographic, substance use, and personality domains. Using multivariate regression, the most parsimonious model found that being male, a non-smoker, and endorsing perseverance was associated with providing DNA. The present study provides benchmark data on sample representativeness in a genome …

Loss Of Smad4 Is Associated With Poor Tumor Immunogenicity And Reduced Pd-L1 Expression In Pancreatic Cancer, Daniel R. Principe, Patrick W. Underwood, Sandeep Kumar, Kaytlin E. Timbers, Regina M. Koch, Jose G. Trevino, Hidayatullah G. Munshi, Ajay Rana

Surgery Publications

Transforming Growth Factor β (TGFβ) is a key mediator of immune evasion in pancreatic ductal adenocarcinoma (PDAC), and the addition of TGFβ inhibitors in select immunotherapy regimens shows early promise. Though the TGFβ target SMAD4 is deleted in approximately 55% of PDAC tumors, the effects of SMAD4 loss on tumor immunity have yet to be fully explored. Using a combination of genomic databases and PDAC specimens, we found that tumors with loss of SMAD4 have a comparatively poor T-cell infiltrate. SMAD4 loss was also associated with a reduction in several chemokines with known roles in T-cell recruitment, which was recapitulated …

Pwatem: An Anthology Of Literature And Art (2022)

Pwatem (Poictesme), 2006-

Pwatem is an anthology of literature and art from undergraduate students at Virginia Commonwealth University. Pwatem publishes poetry, prose and art of all kinds from talented undergraduate students of all majors.

Full Issue

Journal of Mathematics and Science: Collaborative Explorations

No abstract provided.

An Examination Of Factors That Support Sustainable Cultural And Curricular Change In Stem Teaching And Learning, Erica Slate Young, Peaches Hash, Sarah Schott, Jack Bookman

Journal of Mathematics and Science: Collaborative Explorations

Using a mixed-methods design, this body of work from the SUMMIT-P consortium explores possible effective conditions for the sustainable reform of STEM teaching and learning at the collegiate level. A model of catalysts for successful and sustainable change is proposed, based on five years of data collection and observations. These catalysts include institutional support, intrinsic and extrinsic motivation of faculty involved, measures of student success, institution size, prior faculty experience, faculty buy-in, and institutional culture. The discussion ends with a delve into the potential broader impacts of this work. For example, this model may help institutions better understand how to …

A Tale Of Four Departments: Interdisciplinary Faculty Learning Communities Informing Mathematics Education, Bryan D. Poole, Caroline Maher-Boulis, John Hearn, Jason Robinson, Patricia Mcclung, Amanda Jones

Journal of Mathematics and Science: Collaborative Explorations

As a result of the Curriculum Foundations Project and the SUMMIT-P consortium, faculty from four different departments at Lee University created a Faculty Learning Community (FLC) with the goal of improving students’ attitudes toward undergraduate mathematics courses, including students’ perception of the utility of mathematics in their lives and the feelings of anxiety that they experience in these courses. The interdisciplinary collaborations resulted in introducing novel activities and manipulatives in various mathematics courses (Introduction to Statistics, Concepts of Mathematics I and II, and Algebra for Calculus). This paper first describes the efforts of creating the inter-departmental FLC. Second, it discusses …

A Technology-Based, Mixed Methods Approach To Examining The Psychosocial Determinants Of Maternal Health Disparities, Hannah M. Ming

Theses and Dissertations

Background: Exposure to racism and discrimination in the U.S. increases Black women’s risk for experiencing maternal health disparities. Additionally, racism and discrimination affect maternal psychosocial well-being, creating evidence for a biopsychosocial relationship between racism and maternal health outcomes. However, current research does not define the psychosocial Black maternal self well. Given the dynamic relationship between racism, psychosocial well-being, and Black maternal health outcomes, research must comprehensively examine the Black maternal self. The operationalization of a comprehensive construct for Black maternal psychosocial well-being can improve understanding of the relationship between racism, psychosocial well-being, and Black maternal health outcomes.

Purpose: …

Taking Flight: An Independent Living Facility For Emancipated Foster Youth, Kristy Mcdaniel Leitzel

Theses and Dissertations

Motivation

Many of us think about foster care in the context of younger children removed from home. However, little thought is given to youth that age out of the foster care system and the associated challenges and risks they face, namely in the area of housing.

Youth in foster care often receive minimal support in making the transition to independence and are frequently forced into instant adulthood. They are faced with an abrupt end of support at a time when they have not yet mastered the educational, social, or economic survival skills necessary to be independent in the community (Haas, …

Improving Student Knowledge Transfer Between Mathematics And Engineering Courses Through Structured Cross-Disciplinary Collaboration: A Summit-P Initiative, Laura Ellwein Fix, Afroditi Filippas, Maila Brucal-Hallare, Rebecca Segal

Journal of Mathematics and Science: Collaborative Explorations

Student learning across STEM disciplines has been shown to increase with greater integration of applications in mathematics courses. One challenge of this effort is that identical constructs are often presented differently in the partner disciplines than in the mathematics courses. This leads to student confusion and an inability to transfer critical knowledge in their disciplinary courses, even for students who have mastered the mathematical paradigms. An interdisciplinary team at VCU consisting of mathematics and engineering faculty has worked to improve the knowledge transfer required for the integration of applications in the Differential Equations curriculum. This work is part of the …

The Human Louse Microbiome Insights Into Insect-Bacterial Coevolution And Consequences For Vector Capacity, Kirthana Srinivasan

Undergraduate Research Posters

No abstract provided.

Comparison Of Free Versus Encapsulated Drugs On 3t3 Differentiation, Simon H. Friedrich, Gabriel Volpe

Undergraduate Research Posters

The scope of this project was to design, synthesize and test targeted nanoparticles containing hydrophobic and hydrophilic drugs that promote browning in adipose tissue. For hydrophilic drugs the use of liposomes and their hydrophilic core is more useful than the PLGA nanoparticles which have hydrophobic cores. The inhibition of the FOXO1 pathway and modulation of autophagy in adipose tissue can promote browning of white adipose tissue, or an energy burning state where excess energy is burned as heat instead of stored in the cell. If successful, these drugs would offer an alternative treatment for obesity where changes to the patient's …

Neonatal Blood Methylation Marks Associated With Obstetric Pain Relief, Charles J. Tran, Lin Y. Xie, Christina Hultman, Edwin Van Den Oord, Karolina A. Aberg

Undergraduate Research Posters

The placenta, responsible for intrauterine development, can facilitate modifications within the placental epigenome in response to changes in the mother. In turn these changes have the potential to also influence the neonate¹. Pain relief during delivery is widely used and frequently involves the use of nitrous oxide (N₂O, commonly referred to as laughing gas), and pudendal blocks. These treatments, alone or in combination, are generally accepted as safe methods of providing pain relief to mothers. However, laughing gas and local anesthetics such as the ones used during pudendal blocks have been known to cross the placental …

Covid-19 Impact And The Association Between Sociodemographic, Social Support, Depression, And Anxiety In The Mindful Moms Program, Ashley Nguyen

Undergraduate Research Posters

Background: The Covid-19 Pandemic lockdown resulted in lessened social support and a higher incidence of depressive symptoms in the United States. Low-income families were more likely to have experienced negative impacts on income, access to mental health treatment, stress, and discord within the family, and higher anxiety scores during the pandemic. Minority groups had higher COVID-19 incidence and mortality rates. More than 1 in 5 pregnant women in the United States experience clinically significant depressive symptoms, with marginalized populations carrying a greater burden. Maternal depression is linked to poor maternal and child health outcomes.

Depression scores of pregnant individuals during …

Observing Gravitropism In Brassica Rapa Roots, Trang Ly, Maya O'Callagham, Lan Poeschl, Adil Saeed, George Zorn

Undergraduate Research Posters

The objective of this experiment is to observe the effect of gravitropism on the orientation of Brassica rapa (B. rapa) roots. Specifically, amyloplasts that reside at the bottom of root tips act as gravity sensors. As plants are disturbed from upright orientation, roots automatically grow according to the downward pull of gravity. It is hypothesized that as the angle of rotation increases, the angle of root growth is inversely proportional. Five plants containing four seeds of B. rapa were grown over the course of 10 days. Each plates, aside from the control, were rotated once 90°, 80°, and 270° to …

Trajectories Of Medication Non-Adherence With Time-Varying Predictors And Association With Health Outcomes: A Comparison Of Classical Statistical Methods With Machine Learning Algorithms, Vasco Pontinha

Theses and Dissertations

Background: Medication adherence is a major obstacle to improving health care outcomes in long-term therapies for chronic diseases. According to the World Health Organization, interventions for improving medication adherence can have a higher impact on the health of the population than any other advance in medical treatments. Approximately 125,000 individuals die every year in the U.S. because of non-adherence to medication, representing societal costs of $100-289 billion. Previous research has successfully used group-based trajectories methods to identify similar longitudinal medication adherence trajectories. However, medication adherence is not an isolated behavior and is influenced by many factors that current interventions fail …

Comparison Of Peak Skin Dose Calculated By Patient Radiation Dose Monitoring And Tracking Systems Versus Solid-State Detector Measurements, Vincent D. Gargaro

Theses and Dissertations

This study aims to compare the Peak Skin Dose (PSD) that patients obtain from fluoroscopy procedures. The study will show the differences between the PSD measured and calculated from a solid-state detector and the values the unit is displaying. Also, the study will compare PSD measurements from Patient Radiation Dose Monitoring and Tracking Systems (PRDMT). Numerous parameters need to be obtained to depict the PSD that a patient is receiving accurately. The PSD is crucial in assessing the potential biological effects of radiation.

The formalization used to derive the calculated PSD in this study has been established by Jones, A. …

Rational Design Of Metal-Organic Frameworks (Mofs)-Based Functional Materials Towards Better Air Quality, Zan Zhu

Theses and Dissertations

Air pollution is a major threat to environmental safety and public health. Volatile organic compounds (VOCs), particulate matter (PM), and airborne microorganisms are three typical air pollutants. Conventional strategies to prevent and mitigate air pollution have been employed, which, however, are generally passive. For instance, VOC sensing through solid-state devices is a conventional approach, which, however, is not capable of capturing and removing VOCs. On the other hand, air filters and face masks are useful equipment to protect people from inhaling PM and airborne microorganisms. But most commercial filters can only capture them on the surfaces, which may cause secondary …