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Genetic Variations Of Cyp2b6 Enzyme And The Response To Meperidine In Oral Sedation, Sally Hua

Theses and Dissertations

Purpose: The purpose of this study was to determine the relationship of CYP2B6 genotype to the clinical response to meperidine in pediatric dental patients. Methods: Twenty-five patients, ASA I/ II, 45–92 months old, received an oral sedative regimen containing meperidine for dental treatment. The North Carolina Behavior Rating Scale (NCBRS) and Overall Effectiveness of Sedation Scale (OESS) were used to assess their behavior and sedation outcome. Saliva DNA samples were genotyped by PCR-RFLP. Results: We found the following genotype distributions: homozygous wild-type 1*1 (n = 8, 32%), heterozygous 1*6 (n = 13, 52%), and homozygous variant 6*6 (n = 4, …

The Role Of Mitogen-Activated Protein Kinases In The Regulation Of Plant Development, Erica Satterfield

Theses and Dissertations

Mitogen-activated protein kinases are part of an evolutionarily conserved protein phosphorylation cascade which serves essential regulatory functions in eukaryotic organisms. Although the role of MAPKs in the regulation of a plant’s response to environmental stress and plant defense has been well established, very little is known about their role in the regulation of plant developmental processes. In order to examine the role of MAPKs in plant growth and development, a strong mammalian MAPK phosphatase (MKP-1), which is known to inactivate MAPKs in plants, was introduced into tobacco plants. In tobacco plants, MKP-1 overexpression altered plant responses to the phytohormones, ethylene …

Cannabinoid Effects On Nfkappab Function In Microglial-Like Cells: Dual Mode Of Action, Latoya Griffin-Thomas

Theses and Dissertations

Cannabinoids have been shown to modulate the immune system in vitro and in animal models. A major area of interest is how cannabinoids impact the brain. A whole variety of neuropathies or brain disorders, such as AIDS dementia, Parkinson’s disease, Multiple Sclerosis and Alzheimer’s disease, are associated with a hyperinflammatory response within the brain. Microglia, the resident macrophages of the brain, are the major cell type responsible for the persistent elicitation of pro-inflammatory cytokines (IL-1a, IL-1b, IL-6, TNFa) and other mediators. In vitro experiments have demonstrated that the partial exogenous cannabinoid agonist delta-9-tetrahydrocannabinol (D9-THC) and the potent synthetic exogenous cannabinoid …

Commonwealth Times 2009-04-06

Commonwealth Times, 1969-

No abstract provided.

Commonwealth Times 2009-04-02

Commonwealth Times, 1969-

No abstract provided.

Therapeutic Drugs In Cancer And Resistance., Aditi Pandya Martin

Theses and Dissertations

We investigated the mechanism of toxicity and resistance development of small molecule tyrosine kinase inhibitor lapatinib in HCT 116 colon cancer cells. Lapatinib mediated cell death in HCT 116 cells was caspase independent and involved cytosolic release of apoptosis inducing factor. Treatment of HCT 116 cells with 10µM Lapatinib lead to the outgrowth of lapatinib resistant HCT 116 cells. Our studies show that alterations in the expression and activation of Bcl-2 family proteins allow lapatinib resistant HCT 116 cells to resist cytotoxic effects of lapatinib as well as of other commonly used chemotherapeutic agents. In hepatoma and pancreatic cancer cells, …

Commonwealth Times 2009-03-26

Commonwealth Times, 1969-

No abstract provided.

Parental Competencies Of Juvenile Probationers And Adherence To Curt Sanctions And Recidivism Rates, Amy Cook

Theses and Dissertations

The purpose of this exploratory study was to further investigate the notion of parental competencies through the use of the Juvenile Offender Parent Questionnaire as previously developed by Rose and colleagues (2004). The parent questionnaire was administered to 88 parents of juvenile probationers placed on probation in a Virginia county. Exploratory Factor Analysis revealed an eight-factor solution: parental exasperation, parental resignation, mistrust of the juvenile justice system, shame over parenting efficacy, parental monitoring, fear of the child, parent perceptions of child’s exposure to violence, and anger towards child. Regression analyses indicate that parental exasperation and parental resignation were not significant …

Influence Of Parental Substance Abuse On Substance Use And Psychiatric Severity In Drug-Dependent Pregnant Women, Courtney E. Smith

Theses and Dissertations

The purpose of this study was to examine the relationship between history of parental substance abuse and substance use and psychiatric severity measures in a sample of treatment-seeking, pregnant women who meet DSM-IIIR criteria for lifetime opioid and cocaine abuse and/or dependence (N=260). The study examined whether a dose-response relationship was observed between number of parents affected by substance use problems (0, 1, and 2) and degree of women’s substance use and psychiatric severity. Results did not support a dose-response relationship between density of parental problems and substance use and psychiatric severity. However, some differences in severity were seen at …

Binding Studies Of Epidermal Growth Factor Receptor Targeted Compounds Using Surface Plasmon Resonance, Spandana Kankanala

Theses and Dissertations

The study of binding kinetics of proteins plays an important role in understanding molecular mechanisms that drive biological processes. The binding rate constants reflect the dynamics of the system and associated biological activity measurements of the association and dissociation rate constants make it possible to compare different interactions in a standardized manner and help elucidate a mechanistic understanding of binding events. In our study, we used Surface Plasmon Resonance (SPR) technology (Biacore) to study the binding kinetics of the antibodies EGF, Cetuximab and a candidate drug P-13 with the receptor EGFR. The candidate drug P-13 was synthesized and tested on …

Commonwealth Times 2009-03-05

Commonwealth Times, 1969-

No abstract provided.

Shafer Court Connections (2009-03)

Shafer Court Connections, 1994-2010

For alumni of the Academic Campus of Virginia Commonwealth University.

The Approximation Rule: Examining An Alternative To The “Best Interest Of The Child” Custody Standard, Elizabeth Archer

Theses and Dissertations

While the Best Interest of the Child (BIOC) standard has been used since the 1970’s, alternatives such as the Approximation Rule have recently been proposed to remediate perceived weaknesses in BIOC. This study examines the applicability of the Approximation Rule using cross-sectional data collected from 517 children (age 10-18) of divorced parents using the Co-Parenting Behavior Questionnaire (CBQ). Data for all co-parenting and parenting variables (n = 517) were analyzed to determine relationships by time since divorce and custodial relationship. Physical custody was associated with significant differences in children’s’ ratings of both co-parenting and parenting effectiveness. Results for time since …

Commonwealth Times 2009-02-23

Commonwealth Times, 1969-

No abstract provided.

Role Of Rock And Pkc In Regulation Of Contraction Of Permeabilized Femoral Arterial Smooth Muscle, Brendan Browne

Theses and Dissertations

KCl is traditionally used as a stimulus to examine the role of increases in cytosolic Ca2+ on the regulation of smooth muscle contraction. KCl bypasses GPCR activation, thereby avoiding activation of additional cell signaling systems, such as phospholipase C and PKC that are inherent to Gaq and Ga12/13 stimulation. GPCR activation causes Ca2+ sensitization (greater force for a given increase in Ca2+) by a ROCK- and PKC-dependent inhibition of myosin light chain (MLC) phosphatase. Recent studies have demonstrated that KCl can also produce Ca2+ sensitization, implying that Ca2+ itself may induce Ca2+ sensitization. To test the hypothesis that Ca2+ can …

Commonwealth Times 2009-02-02

Commonwealth Times, 1969-

No abstract provided.

Predictors Of Discrepancies In Parents’ And Children’S Reports Of Child Emotion Regulation, Shannon Hourigan

Theses and Dissertations

The ability to effectively regulate one’s emotions has been linked with many aspects of well-being. However, disagreement in parents’ and children’s reports of children’s emotion regulation presents significant measurement and conceptual challenges. This investigation aimed to identify predictors of these discrepancies from among demographic, psychopathology, and child emotional awareness measures and to examine patterns of discrepancies among three emotion types (i.e., anger, sadness, and worry) and three regulation “strategies” (i.e., inhibition, dysregulated expression, and coping). Sixty-one mother-child dyads (41 girls, mean age 9.3 years) participated. As hypothesized, age, child and parent report of psychopathology, and poor emotion awareness all emerged …

Commonwealth Times 2009-01-26

Commonwealth Times, 1969-

No abstract provided.

Commonwealth Times 2009-01-15

Commonwealth Times, 1969-

No abstract provided.

Commonwealth Times 2009-01-12

Commonwealth Times, 1969-

No abstract provided.

The Developmental Effect Of Human Embryoid Bodies (Heb) Under Dynamic Culturing Conditions Using A Perfusion Based Slow Turning Lateral Vessel (Stlv) Bioreactor, Claiborne Collier

Theses and Dissertations

Human embryonic stem cells (hESCs) can provide a unique approach for novel tissue engineering applications. Previous groups have shown that hESCs can differentiate into specialized cell types through the generation of human embryoid bodies (hEBs). These multi-cellular constructs are then subjected to suspension culture for several weeks. Traditional hESC differentiation techniques have yielded non-homogeneous EBs derived in standard static cultures providing an inefficient platform for cellular viability and embryonic modeling. Here, our study aimed at systematically comparing the formation, growth, and differentiation capabilities of hESC-derived hEBs in dynamic and static suspension cultures. We used a continuous flow perfusion slow turning …

Mutations In Many Genes Affect Aggressive Behavior In Drosophila Melanogaster, Alexis C. Edwards, Liesbeth Zwarts, Akihiko Yamamota, Patrick Callaerts, Trudy F.C. Mackay

Psychiatry Publications

Background Aggressive behavior in animals is important for survival and reproduction. Identifying the underlying genes and environmental contexts that affect aggressive behavior is important for understanding the evolutionary forces that maintain variation for aggressive behavior in natural populations, and to develop therapeutic interventions to modulate extreme levels of aggressive behavior in humans. While the role of neurotransmitters and a few other molecules in mediating and modulating levels of aggression is well established, it is likely that many additional genetic pathways remain undiscovered. Drosophila melanogaster has recently been established as an excellent model organism for studying the genetic basis of aggressive …

Cpg Islands Or Cpg Clusters: How To Identify Functional Gc-Rich Regions In A Genome?, Leng Han, Zhongming Zhao

Psychiatry Publications

Background CpG islands (CGIs), clusters of CpG dinucleotides in GC-rich regions, are often located in the 5' end of genes and considered gene markers. Hackenberg et al. (2006) recently developed a new algorithm, CpGcluster, which uses a completely different mathematical approach from previous traditional algorithms. Their evaluation suggests that CpGcluster provides a much more efficient approach to detecting functional clusters or islands of CpGs.

Results We systematically compared CpGcluster with the traditional algorithm by Takai and Jones (2002). Our comparisons of (1) the number of islands versus the number of genes in a genome, (2) the distribution of islands in …

Virginia Commonwealth University Graduate And Professional Programs Bulletin [2009], Virginia Commonwealth University

VCU Bulletins

Graduate and professional programs bulletin for Virginia Commonwealth University for the academic year 2009-2010. It includes information on academic regulations, degree requirements, course offerings, faculty, academic calendar, and tuition and expenses for graduate and professional programs.

A Transcriptional Network Associated With Natural Variation In Drosophila Aggressive Behavior, Shannon Roulet

Psychiatry Publications

Background Aggressive behavior is an important component of fitness in most animals. Aggressive behavior is genetically complex, with natural variation attributable to multiple segregating loci with allelic effects that are sensitive to the physical and social environment. However, we know little about the genes and genetic networks affecting natural variation in aggressive behavior. Populations of Drosophila melanogaster harbor quantitative genetic variation in aggressive behavior, providing an excellent model system for dissecting the genetic basis of naturally occurring variation in aggression.

Results Correlating variation in transcript abundance with variation in complex trait phenotypes is a rapid method for identifying candidate genes. …

Proteomic And Network Analysis Characterize Stage-Specific Metabolism In Trypanosoma Cruzi, Seth B. Roberts, Jennifer L. Robichaux, Arvind K. Chavali, Patricio A. Manque, Vladimir Lee, Ana M. Laura, Jason A. Papin, Gregory A. Buck

Study of Biological Complexity Publications

Trypanosoma cruzi is a Kinetoplastid parasite of humans and is the cause of Chagas disease, a potentially lethal condition affecting the cardiovascular, gastrointestinal, and nervous systems of the human host. Constraint-based modeling has emerged in the last decade as a useful approach to integrating genomic and other high-throughput data sets with more traditional, experimental data acquired through decades of research and published in the literature.

Multiple Splice Defects In Abca1 Cause Low Hdl-C In A Family With Hypoalphalipoproteinemia And Premature Coronary Disease, Jeffrey Rhyne, Myrna M. Mantaring, David F. Gardner, Michael Miller

Internal Medicine Publications

Background Mutations at splice junctions causing exon skipping are uncommon compared to exonic mutations, and two intronic mutations causing an aberrant phenotype have rarely been reported. Despite the high number of functional ABCA1 mutations reported to date, splice variants have been reported infrequently. We screened DNA from a 41 year-old male with low HDL-C (12 mg/dL [0.31 mmol/L]) and a family history of premature coronary heart disease (CHD) using polymerase chain reaction single-strand conformation polymorphism (SSCP) analysis.

Methods Family members with low levels of HDL-C (n = 6) were screened by SSCP for mutations in ABCA1. Samples with altered SSCP …

Estrogen Exposure, Obesity And Thyroid Disease In Women With Severe Pulmonary Hypertension, Lori Sweeney, Norbert F. Voelkel

Internal Medicine Publications

Severe pulmonary hypertension is a lethal group of disorders which preferentially afflicts women. It appears that in recent years the patient profile has shifted towards older, obese, and postmenopausal women, suggesting that endocrine factors may be important. Several studies have revealed an increased prevalence of thyroid disease in these patients, but no studies have evaluated for a coexistence of endocrine factors. In particular, no studies have attempted to evaluate for concurrent thyroid disease, obesity and long-term estrogen exposure in patients.

88 patients attending the Pulmonary Hypertension Association 8th International meeting completed a questionnaire and were interviewed. Information was collected regarding …

A Robust Approach To Identifying Tissue-Specific Gene Expression Regulatory Variants Using Personalized Human Induced Pluripotent Stem Cells, Je-Hyuk Lee, In-Hyun Park, Yuan Gao, Jin B. Li, Zhe Li, George Q. Daley, Kun Zhang, George M. Church

Study of Biological Complexity Publications

Normal variation in gene expression due to regulatory polymorphisms is often masked by biological and experimental noise. In addition, some regulatory polymorphisms may become apparent only in specific tissues. We derived human induced pluripotent stem (iPS) cells from adult skin primary fibroblasts and attempted to detect tissue-specific cis-regulatory variants using in vitro cell differentiation. We used padlock probes and high-throughput sequencing for digital RNA allelotyping and measured allele-specific gene expression in primary fibroblasts, lymphoblastoid cells, iPS cells, and their differentiated derivatives. We show that allele-specific expression is both cell type and genotype-dependent, but the majority of detectable allele-specific expression …

Chaotic Signatures Of Heart Rate Variability And Its Power Spectrum In Health, Aging And Heart Failure, Guo-Qiang Wu, Natalia M. Arzeno, Lin-Lin Shen, Da-Kan Tang, Da-An Zheng, Nai-Qing Zhao, Dwain L. Eckberg, Chi-Sang Poon

VCU Health Publications

A paradox regarding the classic power spectral analysis of heart rate variability (HRV) is whether the characteristic high- (HF) and low-frequency (LF) spectral peaks represent stochastic or chaotic phenomena. Resolution of this fundamental issue is key to unraveling the mechanisms of HRV, which is critical to its proper use as a noninvasive marker for cardiac mortality risk assessment and stratification in congestive heart failure (CHF) and other cardiac dysfunctions. However, conventional techniques of nonlinear time series analysis generally lack sufficient sensitivity, specificity and robustness to discriminate chaos from random noise, much less quantify the chaos level. Here, we apply a …