Digital Commons Network^™

"I Call Them Supermoms": Therapists' Reflections On The Impact Of Youth Sexual Offending On Latina Mothers, Mauricio P. Yabar

Theses and Dissertations

Families of youth who have sexually offended often suffer significant collateral consequences following the discovery of the sexual offense. Samples in research with these families disproportionately consist of mothers and other female parental figures. This is rarely acknowledged in the literature, and the experiences of these mothers have not been explicitly examined. In addition, research on the experiences of these families has largely ignored factors related to race and ethnicity. This is a significant oversight considering the prevalence of systemic racism and discrimination in the United States, particularly in the systems with which these families must interact (e.g., juvenile justice …

Cardiometabolic Genomics And Pharmacogenomics In Filipino Americans: A Cross-Sectional Study, Khalifa Y. Alrajeh

Theses and Dissertations

Background: Heart disease remains a leading cause of death in the country, with significant ethnic disparities. Filipino Americans (FAs), the third-largest Asian subgroup in U.S., face an elevated burden of dyslipidemia, a primary modifiable risk factor for heart disease. Objectives: This community-based study addresses critical knowledge gaps by characterizing the cardiometabolic and genetic factors contributing to dyslipidemia among FAs and implications for personalized cardiovascular drug therapies, particularly statin pharmacogenomics (PGx). Methods: We characterized dyslipidemia prevalence in FAs and major ethnic subgroups using nationally representative NHANES data. We examined associations between uric acid (UA) levels and lipid profiles, …

Covid-19 Vaccine And Infection And The Relationship To Lichen Planus Flare-Ups: A Self-Reported Survey Study, Sara Kube

Theses and Dissertations

Purpose: The aim of this study was to determine if there is an increased occurrence of oral lichen planus flare-ups after COVID-19 vaccination or infection.

Methods: An 11-item questionnaire was dispersed to patients with a diagnosis of oral lichen planus. The survey contained questions regarding the patient’s history of COVID-19 vaccination and infection as it relates subjective oral lichen planus flare-ups (i.e. oral redness, oral ulcerations, and/or oral pain/discomfort).

Results: Self-reported flares were significantly associated with whether or not vaccine side effects were also reported (p-value=0.0053). For those who reported experiencing side effects (tiredness, headache, muscle pain, chills, fever, nausea), …

Family Rejection And Affirmation Experiences Among Racially And Ethnically Marginalized Youth Who Identify As Transgender And/Or Gender Diverse, Angela Matijczak

Theses and Dissertations

Relationships with family members can be a vital source of support, and significant stressor, that contributes to mental health outcomes among transgender and gender diverse young adults who are racially and ethnically marginalized (TGD REMY). Guided by a life history narrative approach, this qualitative study explored family affirmation and rejection among TGD REMY across three aims: (a) how TGD REMY define their experiences of family rejection and/or affirmation, (b) whether and how experiences of rejection/affirmation change throughout the lifetime, and (c) whether and how experiences of rejection/affirmation differ by each family member. Semi-structured interviews were conducted with 17 TGD REMY …

Identifying Mechanistic Pathways To Rigidity Associated With Obsessive-Compulsive Personality Disorder Traits Using A Novel Decision-Making Paradigm, Hannah L. Heintz

Theses and Dissertations

Cognitive and behavioral rigidity is observed across several mental disorders and is a defining characteristic of Obsessive-Compulsive Personality Disorder (OCPD), a highly prevalent and debilitating, yet understudied, disorder. In particular, treatments for OCPD are underdeveloped due to our poor understanding of the mechanisms leading to the disorder’s key feature of rigidity. Two related disorders, anorexia nervosa (AN) and obsessive-compulsive disorder (OCD), have shown distinct mechanistic pathways leading to symptoms of rigidity, indicating that this trait can arise as a result of a number of differentially impaired cognitive processes, each requiring unique interventions. To examine the relationship between symptoms of OCPD …

Utilizing Ai Integrated Neuroimaging Technology To Expand Upon Machine Learning In Positron Emission Tomography Technology With The Aim Of Detecting Amyloid Beta Biomarkers Early In The Onset Of Alzheimer's., Ethan S. Terman

Undergraduate Research Posters

Early intervention in Alzheimer's is vital for treatment. The earlier a professional can detect symptoms and make a diagnosis the earlier a prognosis can be implemented. With the prevalence of data in our day-to-day world combined with Artificial intelligence (AI), utilizing both for machine learning can pave the way for more accurate and efficient detection of Alzheimer's and other neurodegenerative diseases. AI combined with Machine learning (ML) increases diagnostic efficiency and reduces human errors, making it a valuable resource for physicians and clinicians alike. With the increasing amount of data processing and image interpretation required, the ability to use AI …

How Does A Global Climate Cycle Affect The Reproductive Output Of A Neotropical Migratory Bird?, Abby K. Bressette, Emma Cummings, Daniel Albrecht-Mallinger, Lesley Bulluck

Undergraduate Research Posters

El-Niño Southern Oscillation (ENSO) causes regional changes in climate and has been found to have varying effects on the survival, fecundity, and recruitment of migratory songbirds. This study seeks to better understand the effect of ENSO on the fecundity of Virginia-breeding Prothonotary warbler (Protonotaria citrea; PROW), a neotropical migratory songbird. PROW breed in the Eastern United States and spend the non-breeding season primarily in Panama and Colombia. Ongoing research in Virginia has found a positive relationship between spring precipitation and predicted number of PROW fledgelings during La Niña, but the opposite during El Niño. To better understand this relationship, we …

Lycosidae Abundance And Diversity Across Lawn And Leaf Litter Substrate, Sage O. Lockett, Dan Albrecht-Mallinger

Undergraduate Research Posters

Significant knowledge gaps exist in how land-cover impacts ground-hunting spider populations. To fill these gaps, this study investigates a common family of ground-hunting spiders, Lycosidae, to determine differences in their abundance and diversity in deciduous leaf litter and managed turfgrass (lawn). The study was conducted within a forested ecosystem at Virginia Commonwealth University's Rice Rivers Center in Charles City County, Virginia. I placed 10 belt transects (1m x 20m) on lawn substrate and 10 identical transects in deciduous forest leaf litter substrate. I performed repeated visual census via eyeshine and manual capture of up to three individuals per transect …

Illuminating Function And Enabling Inhibition At The Protein-Membrane Interface Of Glutathione Peroxidase 4., Courtney L. Labrecque

Theses and Dissertations

Membrane proteins act as gateways to cells and are responsible not only for signaling, lipid transport, and cellular homeostasis, but they are also integral in disease pathologies. One class of membrane proteins, peripheral membrane proteins (PMPs), reside in aqueous compartments of the cell until they are recruited to the membrane to perform their function. Glutathione peroxidase 4 (GPx4) is a PMP enzyme responsible for engaging the membrane and reducing lipid hydroperoxides, thereby preventing cellular death via ferroptosis. Due to its central function surrounding ferroptosis, GPx4 is an interesting drug target that has recently gained interest for inflammatory diseases as well …

Latent Profiles Of Child Coping And Perception Of Experience Processes To Predict Long-Term Symptoms Of Depression, Intrusive Grief, And Suicidality Outcomes In Parentally Bereaved Children, Rebecca Hoppe

Theses and Dissertations

Childhood bereavement is a significant public health issue and the most distressing form of childhood trauma. The death of a parent can disrupt the course of a children's development and potentially predispose them to enduring adverse mental health outcomes, such as depression, intrusive grief, and suicidality. Recognizing grief as a dynamic process, theories have suggested that children’s unique coping and perceptions of experience can contribute to variations in individual adaptations to the death and the onset of mental health problems. The goals of this study were to (a) use latent profile analysis to empirically group recently bereaved children into distinct …

Outcomes Of Anterior Crossbite Correction On Alveolar Bone Morphology In Patients With Cleft Lip And Palate, Katherine J. Hayes

Theses and Dissertations

Objective: To determine if patients with cleft lip and/or cleft palate (CLP) with dental correction of the anterior crossbite during growth display differences in alveolar bone thickness around the maxillary central incisors compared to patients without crossbite correction.

Methods: This retrospective study evaluated post-treatment cone beam computed tomography (CBCT) images of growing patients with CLP with anterior crossbites who underwent Phase I orthodontic treatment (n=32). CBCTs were sorted by whether the crossbite was corrected (n=16) or not corrected (n=16) during Phase I. Alveolar bone thickness was measured on the buccal and palatal aspects of the central incisors at 3 mm, …

The Effects Of Various Nanoplastics On The Inflammatory Response In Primary Alveolar Epithelial Cells, Eunice J. Pak

Theses and Dissertations

As plastic pollution begins to multiply in the environment and the workplace, gradual degradation of these plastics creates an unseen threat to biological health: nanoplastics. These nanoplastics are common in day-to-day life, and have been found in various internal organs of the human body. The biological and health effects of these nanoplastics are still widely unknown, and only recently has research focused on the impacts of these particles on human health at the cellular level.

In this thesis, poly(methyl methacrylate) and three forms of polystyrene (carboxyl-modified, amine-modified, and neutral surface charge) have been tested on submerged primary C57BL/6 mouse alveolar …

The Effect Of Experienced Compassion At Work On Nursing Home Certified Nursing Assistants' Well-Being And Turnover Intentions, Kristin M. Macdonald

Theses and Dissertations

Statement of problem and objectives: Could experienced compassion at work alleviate nursing home certified nursing assistant (CNA) burnout? This study investigates whether experienced compassion at work predicts higher well-being (meaningfulness of work, psychological vitality, and resilience) for CNAs, and if well-being predicts lower turnover intentions.

Procedure followed: This is a prospective, observational, survey-based study with three timepoint data collection. Convenience sampling of Kansas and Virginia NHs was conducted (n=13). All CNAs working at the NHs were study eligible. CNAs received gift cards with laddered incentives for ongoing participation. There was in-person administration of a CNA survey at each timepoint. …

The Effect Of 17Β Estradiol On Local Production Of Active Vitamin D3 Metabolites In Laryngeal Squamous Cell Carcinoma, Tillat Batool

Theses and Dissertations

Laryngeal squamous cell carcinoma (LSCC) has garnered attention as a sex-hormone-dependent cancer with notable responsiveness to 17β-estradiol (E₂), in which it’s tumorigenesis can be mediated through estrogen receptors (ER). The ESR1 gene encodes for the classical full-length ERα66, as well as splice variants ERα36 and ERα46. Previous studies have shown that an active vitamin D₃metabolite, 24R,25(OH)₂D_3. has pro-tumorigenic or anti-tumorigenic responses in LSCC cells with different ER profiles, and that the tumor cells have the capability of localized production of vitamin D metabolites at the cellular level when treated with 25(OH)D₃-d6 …

Re-Programming Transcription Factor Function For Neuroscience Research Of Addiction: Investigating The Role Of Zinc Finger Proteins In Driving Cocaine Reinforcement In Mice, Joseph A. Picone

Theses and Dissertations

Administration of addictive drugs like cocaine or morphine initiates aberrant gene transcription within brain reward circuitry neurons, which contributes to the lasting behavioral maladaptations that define addiction. The drug-induced expression and function of key brain transcription factors (TFs) is one major mechanism through which these drugs are able to regulate transcription, and as a consequence, lasting damaging drug-related behaviors including compulsive drug use. The goal of this dissertation is to more fully understand the molecular mechanistic drug-specific actions of TFs within the rodent nucleus accumbens (NAc). The findings from these studies could serve as the basis to identify novel candidate …

A Multilevel, Mixed Methods Examination Of Food Insecurity, Housing Instability, And Cardiovascular Health, Sarah Collins

Theses and Dissertations

BACKGROUND

Housing instability and food access are both robust risk factors for cardiovascular disease (CVD), which claims more lives annually in the U.S. than cancer and chronic lung disease combined. We hypothesized that experiencing both housing instability and food insecurity would have an additive effect on CVD risk, resulting in elevated blood pressure (BP) and perceived stress among young adults (YA).

METHODS

This research examined the relationships between food insecurity, housing instability, stress, and cardiovascular disease risk through three aims. First, multivariate linear regression was used to assess associations among YA, focusing on eviction, food insecurity, and blood pressure. Second, …

Transgender Perceptions Of Breast Health Care And Utilization Of Preventative Breast Imaging Services, Cherish Parham

Theses and Dissertations

Little information exists on transgender patient perceptions of breast imaging care at mammography facilities. The purpose of this descriptive phenomenological study was to understand the lived experiences of transgender patients at mammography facilities, their knowledge of breast care, and how these experiences have shaped their perceptions of the breast imaging community and attitude toward breast cancer screening. By applying the revised the Gelberg-Andersen Behavioral Model for Vulnerable Populations, this study addressed factors specific to the transgender population that may affect their healthcare utilization and how that impacts health outcomes. Researchers collected data from transgender/non-binary individuals in the Richmond, Virginia region …

Analyzing The Role Of Slc25a32 In Hepatocellular Carcinoma, Mimansha Shah

Theses and Dissertations

Hepatocellular carcinoma (HCC) is the most common primary liver cancer and a leading cause of cancer-related deaths worldwide. Despite advances in understanding its molecular pathogenesis, treatment options for HCC remain limited, highlighting the need for identifying novel therapeutic targets. This study investigates the role of SLC25A32, a mitochondrial transporter gene located on chromosome 8q22.3, in HCC progression. SLC25A32 is involved in the transport of tetrahydrofolate (THF) and flavin adenine dinucleotide (FAD) into mitochondria, playing a crucial role in one-carbon metabolism and redox balance. Using CRISPR/Cas9 technology, we generated potential SLC25A32 knockout clones in the HCC cell line Huh-7. Quantitative PCR …

Shape Inverse Prediction Of Magnetic Field-Actuated Soft Robots By Neural Network Machine Learning, Lineth J. Perez Monsalve

Theses and Dissertations

Soft robotics has drawn tremendous interest in recent years because the compliance and motion of soft robotics enable biocompatibility and versatility for many applications, such as human-machine interaction, wearable and assistive devices, and health monitoring. This study introduces a novel predictive modeling approach using neural networks for shape control of magnetic soft robots. The robots are made of silicone materials embedded with hard magnetic particles, which respond to the external magnetic field provided by a ring-type of permanent magnet. These robots, free from physical connections to external devices, i.e., non-tethered actuation, hold significant potential for applications in healthcare, such as …

Role Of Human Intermediate Form Prl Receptor I-Tail In The Pathogenesis Of Breast Cancer, Shanwei Shen

Theses and Dissertations

Breast cancer continues to be the second most common cancer in women in the United States. The neuroendocrine hormone human prolactin (hPRL) plays an important role in normal mammary gland development and growth, stimulating normal breast tissue proliferation and differentiation. Considerable evidence has shown that hPRL is involved in the malignant transformation of human breast tissue. hPRL enhances viability, invasiveness, and proliferation of breast cancer cells in vitro. Evidence from epidemiologic and genetic studies also implicates role of hPRL in the pathogenesis of breast cancer. Its cognate receptor the human prolactin receptor (hPRLr) is required for the action of hPRL. …

Fat And Water Content Effects Of Three Food Substrates (Ground Veal, Ground Beef, And Beef Liver) On Lucilia Sericata (Diptera: Calliphoridae) Development And Mortality, Nicole Dailey

Master of Science in Forensic Science Directed Research Projects

Although temperature has been identified as the most important catalyst affecting blow fly larval development, other variables encountered by these species, such as the fat and moisture content of the food source, may have an impact on accurate estimations of the PMImin in forensic investigations. Thus, body composition differences between human carrion, such as variations in body fat and water percentages between younger and older individuals, or heavier persons, could affect blow fly developmental rates, and in turn cause over or under estimations of the PMImin. In this study, the weight, length, and development times of Lucilia sericata third instar …

The Development And Evaluation Of A Method For Complete Collagenase I Digestion Of Bos Taurus Bone Samples, Reilly Price

Master of Science in Forensic Science Directed Research Projects

Current methods for processing aged forensic bone samples for DNA analysis involve pulverization and organic extraction, which further destroys already degraded DNA and uses large amounts of limited samples. Additionally, the success rates for this type of analysis vary widely as homogenization incorporates PCR inhibitors into the sample. Little research has explored how pulverization results in DNA damage in profiles obtained from skeletal remains. This study proposes an enzymatic digestion method to separate osteocytes from PCR inhibitors in the extracellular bone matrix to improve the quality and quantity of DNA isolated from aged bone samples. The technique described here used …

A Comparative Analysis Of Swabbing Solutions For Sexual Assault Evidence: Direct Impact On Spermatozoa And Dna Recovery, Olivia Q. Kassie

Master of Science in Forensic Science Directed Research Projects

Forensic laboratories struggle with a backlog of sexual assault cases, prompting the need for effective techniques to develop consistent protocols and address scientific gaps. Challenges in recovering optimal DNA from assault evidence stem from factors like collection timing and sampling variables. Variations in lab protocols can lead to differences in DNA yields and profile success. This was observed in a state lab when protocols were developed allowing the use of acetone to swab sexual assault evidence. The use of acetone originated due to their case-routing protocols in multi-section cases involving latent fingerprints. While not necessary for sexual assault and porous …

Cellular Autofluorescence Signatures For Determining Time Since Deposition Of Whole Blood, Hannah Lamer

Master of Science in Forensic Science Directed Research Projects

Knowledge of the relative window of time that evidentiary biological material was deposited at a crime scene, referred to as the time since deposition (TSD), would help investigators determine which evidentiary items are most probative and assist with scene reconstruction. One commonly encountered body fluid at a crime scene is blood. Various methods for estimating TSD of bloodstains have been developed, but none to date have been validated for use in the forensic laboratory. Recently, flow cytometry measurements of cellular autofluorescence and size/structure have been investigated as a signature for TSD of saliva and touch epidermal cells. This study assessed …

Overcoming The Effects Of Oily Contaminants On Dna Extraction Efficiency Using Dna Iq™ System, Elizabeth G. Gillece

Master of Science in Forensic Science Directed Research Projects

In forensic science DNA extraction and purification are crucial to DNA analysis as evidentiary items vary in DNA quantity and quality. In casework the goal to is maximize DNA yield, remove PCR inhibitors, and develop a full profile while preserving evidence. Extraction methods are developed to target the DNA for optimal isolation. Solid-phase extraction is advantageous as it can extract DNA from a variety of tissue types, it is amenable to automation, and it provides quick and efficient purification of DNA. Solid-phase extraction methods may have complications with contaminants that hinder the quantity and quality of the DNA recovered. In …

A Novel Dual Configuration Dart/Uhplc-Ms/Ms System For The Rapid Analysis Of Psilocybin-Containing Mushrooms And Other Seized-Drug Submissions, Aron R. Jaffe

Master of Science in Forensic Science Directed Research Projects

The use and availability of controlled substances is highly dynamic. When a classical or novel psychoactive substance becomes popular, an influx of seized-drug evidence submissions closely follows, contributing to an ever-growing backlog of exhibits awaiting identification. The need for inclusive, rapid, and robust analytical methods is apparent, as demonstrated by recent growth in hallucinogen use. Psilocybin and psilocin, among other entheogens, have become increasingly popular and an increase of psilocybin mushroom seizures by law enforcement has followed. The structural similarity and thermal lability of many entheogens preclude their identification using many conventional analysis techniques. A DART and UHPLC were interchangeably …

Using The Microbiome To Assess The Health Status Of The Individuals Recovered From The East Marshall Street Well, Arian Karim

Master of Science in Forensic Science Directed Research Projects

In 1994, during the construction of the Kontos Building on the Medical College of Virginia Campus, a well on East Marshall Street was discovered to contain artifacts and human remains of African descent. Upon archaeological analysis by the Smithsonian, the ancestral remains can be dated back to the mid-19th century, when grave robbing and the use of cadavers for anatomical purposes [BS1] in medical schools were common. The human oral microbiome is the second largest human microbiota, containing over 600 species of organisms. Dysbiosis of the oral microbiome can lead to the overgrowth of pathogenic species, resulting in illness and …

Temporal Discrimination Of Lucilia Sericata Pupae Using Dart- Tof-Ms And Statistical Analysis, Nguyen-Khoa N. Pham

Master of Science in Forensic Science Directed Research Projects

Lucilia sericata (Diptera: Calliphoridae) is a forensically important blow fly species. These flies have been extensively studied as they are one of the earliest colonizers of corpses. Adult females blow flies lay approximately 200-2,300 eggs, which upon hatching will feed off the body as they metamorphosize from first to second, and third instars. The third instar larvae then migrate away from the body in a stage known as pre-pupa, and develop into the puparial stage. The adults emerge from the pupa stage, leaving behind their empty puparium. These developmental stages are used by forensic entomologists to determine a minimum postmortem …

Quantitation And Visualization Of Epithelial Cells On Cartridge Cases, Paige Kibler, Christopher Ehrhardt, Katherine M. Philpott, Jody H. West, Stephanie Walcott

Master of Science in Forensic Science Directed Research Projects

Fired cartridge cases are a common type of evidence submitted to forensic DNA profiling but are challenging to analyze because they usually contain only trace amounts of biological material. As such, there is a significant need in caseworking units for new screening techniques to determine if biological material is present and is in high enough quantity to attempt DNA profiling. The goal of this study was to test a new method for quickly visualizing biological material on cartridge cases. Biological deposits were created by handling fired and unfired cartridge cases through a series of conditions. The biological material was collected …

Assessing The Effectiveness Of Alternative Light Sources In The Search For Skeletal Remains, Oiivia L. Thompson

Master of Science in Forensic Science Directed Research Projects

Many search and recovery operations for human skeletal remains are unsuccessful due to difficulties recognizing bones in outdoor environments even when evidence indicates the last known whereabouts of a missing individual. Though the collagen component of bone is known to emit fluorescent, this property has not been leveraged consistently during skeletal remains searches. Thirty-six mock searches were completed in 5000 ft² zones of eastern deciduous forest by volunteers associated with the Virginia Department of Emergency Management. Pig and deer bones were scattered and partially concealed under brush and leaf cover. Pairs of volunteers were allowed up to one hour …