Digital Commons Network^™

Protective Factors In The Association Between Child Sexual Abuse And Telomere Length In Adults, David Sosnowski

Theses and Dissertations

The purpose of the present study was to examine if childhood sexual abuse (CSA) was associated with decreases in mean telomere length (TL), and if social support and/or optimism moderated this association. The study included 99 Caucasian female monozygotic twins, ranging in age from 19-48 (M_age = 30.5, SD = 7.8) at Time 1. Linear mixed effects models were employed to test study hypotheses. Analyses with all participants did not detect an effect of CSA exposure or severity on mean TL, nor were there effects with optimism. However, in analyses that only included women exposed to abuse, increases …

Sol-Gel Chemistry: An Advanced Technique To Produce Macroscopic Nanostructures Of Metal And Semiconductor Colloids, Lamia Nahar

Theses and Dissertations

The fascinating physical properties that arise in materials limited to dimensions of 1-100 nm have gained noteworthy interest from the scientific community. Accordingly, there has been a lot of attention paid to the synthesis of discrete nanoparticles (NPs) and they are being investigated for a range of advanced technologies. Nonetheless, efficient use of nanomaterials in device applications require them to be assembled into solid state macro-structures while retaining their unique, nanoparticulate properties. To date, most commonly investigated assembling techniques include: covalent coupling of NPs surface groups, control evaporation of the solvent to produce ordered supercrystals or non-ordered glassy films, and …

Po2 Dependence Of Oxygen Consumption In Skeletal Muscle Of Diabetic And Non-Diabetic Rats, Alexander C. Liles

Theses and Dissertations

Abstract

PO₂ DEPENDENCE OF OXYGEN CONSUMPTION IN SKELETAL MUSCLE OF DIABETIC AND NON-DIABETIC RATS

By: Alexander C. Liles

A thesis submitted in partial fulfillment of the requirements for the degree of Master of Science at the Medical College of Virginia Campus, Virginia Commonwealth University

Virginia Commonwealth University, 2017

Advisor: Roland N. Pittman, Ph.D.

Department of Physiology and Biophysics

Type 2 diabetes mellitus (T2DM) is a major medical problem around the world, affecting nearly 6% of the world’s population. This study was an attempt to better understand physiological changes the disease may cause to the microcirculation and more specifically, to …

A Novel Synthesis And Subsequent Decyclization Of Iminothiozolidinones: Expansion Of Thiourea Chemistry For Biological Applications, Constance D. Franklin

Theses and Dissertations

Small molecule synthesis has become a valuable tool in the study of biological systems. Biologically active compounds can be designed based on well-characterized endogenous systems or they can be found through the screening of large libraries of small molecules. This work involves the development of a small library of cyclic thiourea-based small molecules by use of an unreported synthetic pathway. Briefly, parent thioureas were cyclized by reaction with bromoacetyl bromide, and one or two isomeric heterocycles were found to form. Further studies indicated that the reaction could be easily manipulated by temperature or solvent to effectively control the product distribution. …

Synchrony And Concordance: A Multilevel Analysis Of The Effects Of Individual Differences During A Co2 Challenge, Rachel E. Wallace

Theses and Dissertations

Emotion theories posit that emotion systems (e.g., behavior, self-report, physiology) should be related when an emotion is being elicited because this serves an adaptive purpose and allows the individual to respond appropriately to the present situation. Oftentimes, this coherent relationship is not found, and research has hypothesized that the type of analyses used and lack of examination of individual differences could be affecting this relationship. Most studies examine the relationship between emotion systems between-subjects when within-subjects analyses may be more appropriate. The present study examined the relationship between self-reported distress (SUDS) and heart rate, and whether trait differences of anxiety …

A Range-Wide Assessment Of Migratory Connectivity For The Prothonotary Warbler Using Stable Hydrogen Isotopes, Jessica Reese

Theses and Dissertations

Populations of many species of migratory birds are declining, and an understanding of how populations are linked between the breeding and nonbreeding grounds is necessary in order to determine drivers of declines. While all current tracking technologies to study the movements of small songbirds are limited by their coarse resolution, tracking birds by measuring stable isotopes in keratin offers an advantage because it requires only a single capture and is cost-effective, which leads to robust sample sizes. While this tracking method is accurate, stable hydrogen isotope values measured in feathers (δ²H_f) are known to be variable …

The Institute Of New Feelings: Plastic Identities And Imperfect Surfaces, Weijian Zhou

Theses and Dissertations

Digital media are moldable spaces where an image is simultaneously a thought. This instance and flexibility enables digital existences to be malleable, transformative, situational, and unstable. They are plastic images. Video games generate digital bodies that are a fusion of subjectivities and cybernetic simulations, in a perceivable and ambiguous process. Such bodies are extensions of ourselves, being girlish, imperfect, unfinished and happening—digesting and emitting clusters of feelings, regardless of our biological gender and age. The performative experience of play is progressively departing from spectacle, gambling and competition, and increasingly shifting towards an emotional journey of alternate realities, spreading subjectivities into …

Comparison Of Intraoral And Extraoral Scanners On The Accuracy Of Digital Model Articulation, Jason L. Porter

Theses and Dissertations

Introduction: Orthodontists increasingly rely on digital models in clinical practice. The ability of modern scanners to articulate digital models must be scientifically evaluated. Methods:Twenty five digital articulated models were produced from four digital scanners in five experimental groups. The resulting inter-arch measurements were compared to the gold standard. An acceptable range of 0.5mm more or less than the gold standard was used for evaluation. Results: iTero® and iTero® Element yielded all acceptable inter-arch measurements. The 3M™ True Definition and Ortho Insight 3D® with Regisil® bite registration produced four of six acceptable inter-arch measurements. The Ortho Insight 3D® with Coprwax ™ …

A Multi-Method Exploration Of The Genetic And Environmental Risks Contributing To Tobacco Use Behaviors In Young Adulthood, Elizabeth K. Do

Theses and Dissertations

Tobacco use remains the leading preventable cause of morbidity and mortality in both the United States and worldwide. Twin and family studies have demonstrated that both genetic and environmental factors are important contributors to tobacco use behaviors. Understanding how genes, the environment, and their interactions is critical to the development of public health interventions that focus on the reduction of tobacco related morbidity and mortality. However, few studies have examined the transition from adolescent to young adulthood – the time when many individuals are experimenting with and developing patterns of tobacco use. This dissertation thesis seeks to provide a comprehensive …

Chemical Probes For Protein Α-N-Terminal Methylation, Brianna D. Mackie

Theses and Dissertations

While protein α-N-terminal methylation has been known for nearly four decades since it was first uncovered on bacteria ribosomal proteins L33, the function of this modification is still not entirely understood. Recent discoveries have demonstrated α-N-terminal methylation is essential to stabilize the interactions between regulator of chromosome condensation 1 (RCC1) and chromatin during mitosis, to localize and enhance the interaction of centromere proteins (CENPs) with chromatin, and to facilitate the recruitment of DNA damage-binding protein 2 (DDB2) to DNA damage foci. Identification of N-terminal methyltransferase 1 (NTMT1) unveiled the eukaryotic methylation writer for protein α-N-termini. In addition, NTMT2 that shares …

Development Of My Footprint Calculator, Karthik Mummidisetti

Theses and Dissertations

The Environmental footprint is a very powerful tool that helps an individual to understand how their everyday activities are impacting environmental surroundings. Data shows that global climate change, which is a growing concern for nations all over the world, is already affecting humankind, plants and animals through raising ocean levels, droughts & desertification and changing weather patterns. In addition to a wide range of policy measures implemented by national and state governments, it is necessary for individuals to understand the impact that their lifestyle may have on their personal environmental footprint, and thus over the global climate change. “My Footprint …

In Search Of A Function For An Uncharacterized Conserved Protein In Streptococcus Sanguinis Sk36, Ayana Scott-Elliston

Theses and Dissertations

With the number of fully sequenced bacterial genomes increasing in the past 7 years, it has been discovered that a large percentage of the putative protein coding genes have no known function. This lack of knowledge leaves scientists with an incomplete understanding of bacteria. In this study, conserved hypothetical protein mutants from Streptococcus sanguinis SK36 were screened on solid media with various environmental conditions. From these screens, the candidate protein, SSA_2372, displayed a sensitivity to acidic conditions. Its homolog in Bacillus subtilis 168, BSU00030, also displayed a sensitivity to pH conditions at its acid tolerance extremes unlike its other homolog …

Effects Of Prescribed Fire On Cope’S Gray Treefrog (Hyla Chrysoscelis) Across Habitat Scales And Life Stages, Logan Mcdonald

Theses and Dissertations

Fire may alter both aquatic and terrestrial habitat used by all amphibian life stages, yet, our knowledge of its effects on amphibians is primarily limited to adult responses. I present an integrated approach to test the response of Cope’s Gray Treefrog (Hyla chrysoscelis) to fire by examining responses in tadpole performance and survivorship, adult abundance, and oviposition. Tadpoles raised with burned leaf litter had similar survival, but total mass and total length were 440% and 170% greater, respectively, for tadpoles raised in unburned litter. I assessed terrestrial and aquatic oviposition cues by embedding burned and unburned litter treatments within burned …

Comparisons Of Isogenic Trisomic And Disomic Cells From People With Mosaicism For Down Syndrome Unmask Cellular Differences Related To Trisomy 21, Kelly A. Rafferty

Theses and Dissertations

It is known that age-related changes impacting multiple organ systems occur earlier in people with Down syndrome (Ds), but the biological basis underlying this trisomy 21-associated propensity for premature aging is poorly understood. Given that the trisomic/normal cells from people with mosaic Ds (mDs) are identical with regards to environmental exposures and genes (except for chromosome 21 copy number), comparisons of these isogenic trisomic/disomic cells allow one to “unmask” the cellular consequences of trisomy 21 by removing extraneous factors. The primary aim of this study was to determine if trisomy 21 results in an increase in the acquisition of age-related …

D Is For The Most Cherished Sense (Whence It Comes And Wither It Goes), Hallie S. Mcneill

Theses and Dissertations

A transcript of the audio that constitutes the work by the same title, along with an introduction and relevant bibliography.

Dredging Land Reclamation Causing Mucus Development In Massive Spherical Corals In The Spratly Islands, South China Sea: The Effects On China’S Fishing Industry, Tiffany K. Ho

Undergraduate Research Posters

Images from Asia Maritime Transparency Initiative depicted huge masses of white coral mucus secreted from coral reefs in the Spratly Islands of the South China Sea, an area where several land reclamation projects are occurring. The purpose of this research was to understand how increased sedimentation levels from the dredging land reclamation method affects mucus development in massive corals, such as Acropora, located in these reefs. Journal articles that focused on the biology of coral mucus, bacterial communities associated with coral tissue, mucus, and seawater, coral mortality from different environment impacts, coral coverage of reefs in Spratly Islands, and symbiotic …

Characterization Of A Putative Hemolysin Expressed By Sneathia Amnii, A Preterm Birth-Associated Pathogen, Lizette Carrasco, Kimberly Jefferson

Undergraduate Research Posters

The gram-negative bacteria Sneathia amnii is a poorly-characterized commensal of the female urogenital tract frequently associated with adverse clinical outcomes such as bacterial vaginosis (BV), amnionitis, and preterm labor. To investigate its potential role in virulence, we sought to identify and characterize virulence determinants produced by S. amnii in an effort to better understand the pathogenesis of infectious preterm birth. Through sequencing of the Sn35 genome (type strain of S. amnii), we identified two genes with amino acid sequence similarity and structural similarity to the filamentous hemagglutinin (FHA) protein of Bordetella pertussis and its Type Vb transporter. Because S. amnii …

The Extension Of Rbc Longevity And Functionality In The Prevention Of Graft Versus Host Disease, Shanmuka Gadiraju, Megh Kumar

Undergraduate Research Posters

Given today’s current scientific method of preservation, red blood cells (RBCs) donated or drawn from live humans have a storage life of approximately 42 days, after which the blood will be discarded due to of degradation of the RBCs. The mechanism that drives said degradation is known as oxidative stress, in which the cells’ inability to balance out the creation and excretion of free radicals causes a conformational change in the shape and efficacy of RBCs. In order to counteract the oxidative actions upon the cells, it has been thought that the addition of reducing agents, specifically ascorbic acid, the …

Separation Of Blood Mixtures Using Fluorescently Labeled Antibodies, Christopher Ehrhardt, Dani Jabado, Emily Brocato

Undergraduate Research Posters

Identifying and analyzing biological mixture samples at a crime scene are of paramount concern for forensic scientists, especially if that type of evidence contains only one cell type. The presence of multiple contributors in a biological evidence sample reduces the probative value of DNA evidence and can sometimes lead to its eventual loss of value. As such, this study was performed in an attempt to examine and evaluate flow cytometry analysis as a means to separate blood mixture samples labeled with fluorescent antibodies. Fluorescein Isothiocyanate (FITC) antibodies were specifically targeted and bound to HLA (Human Leukocyte Antigens) markers present on …

Separation Of Blood Mixtures Using Fluorescently Labeled Antibodies, Dani Jabado

Undergraduate Research Posters

Identifying and analyzing biological mixture samples at a crime scene are of paramount concern for forensic scientists, especially if that type of evidence contains only one cell type. The presence of multiple contributors in a biological evidence sample reduces the probative value of DNA evidence and can sometimes lead to its eventual loss of value. As such, this study was performed in an attempt to examine and evaluate flow cytometry analysis as a means to separate blood mixture samples labeled with fluorescent antibodies. Fluorescein Isothiocyanate (FITC) antibodies were specifically targeted and bound to HLA (Human Leukocyte Antigens) markers present on …

Evolution Of Protein Complexes In Bacterial Species, Shwetha Hara Sridhar, Wedad Albalawi, Peter Uetz

Undergraduate Research Posters

Protein complexes are composed of two or more associated polypeptide chains that may have different functions. Protein complexes play a critical role for all processes in life and are considered as highly conserved in evolution. In previous studies, protein complexes from E. coli or Mycoplasma pneumoniae have been characterized experimentally, revealing that a typical bacterial cell has on the order of 500 protein complexes. Using gene homology (orthology), these experimentally-observed complexes can be used to predict protein complexes across many species of bacteria. Surprisingly, the majority of protein complexes is not conserved, demonstrating an unexpected evolutionary flexibility.

The current research …

Isolation Of A Novel Phage Otoolekemple52, Thomas O. Raymond

Undergraduate Research Posters

A bacteriophage is a virus capable of infecting bacteria like ubiquitous soil-dwelling genus Bacillus. Within the Bacillus genus, there is the “ACT family” made up of B. thuringiensis, B. cereus, and B. anthracis, which are highly related but with different pathogenic characteristics. Because of this, phages isolated using a species in this group may have a broad host range encompassing several species from Bacillus. Since B. cereus and B. anthracis can result in fatal to mild sickness in humans, the non-pahtogenic B. thuringiensis kurstaki was used to discover and characterize novel phages. The phage OTooleKemple52 was isolated …

Meta-Analysis Of The Origin Of Bimaturism In Orangutan Males, Mina Adnan

Undergraduate Research Posters

Unlike any other primate species, orangutans exhibit extraordinary sexual differences in their facial morphology. Two different strategies are available for males that reach sexual maturity: either become fully “flanged” and develop secondary sexual characteristics, which is knownto be only developed by the dominant male, or remain “unflanged” and not develop secondary sexual characteristics. The mechanism for how this bimaturism evolved and how occurs is poorly understood, but both flanged and unflanged males are reproductively successful.

This project explores the physiological mechanism behind bimaturismin orangutan males; namely, are there genetic differences between flanged and unflanged males such that the strategy is …

Re-Imagining Inclusion/Exclusion: Unpacking Assumptions And Contradictions In Arts And Special Education From A Critical Disability Studies Perspective, Alice J. Wexler

Journal of Social Theory in Art Education

Inclusion is usually defined “as a student with an identified disability, spending greater than 80% of his or her school day in a general education classroom in proximity to nondisabled peers” (Baglieri et al., 2011, p. 2125). This term, although seemingly benign and even beneficial, is nevertheless the outcome of polarized and divided terminologies. As a result, inclusion within the public school system can suggest not belonging. In this article I examine the invisible barriers to children’s full inclusion and participation hidden within the terminology and practices of special education, and suggest how the arts might be a natural ally …

The Ceiling Is The Sky: Affective Constructs, Event, And Community In The Marginal Spaces Of Art Education, Kristopher J. Holland, Nandita Baxi Sheth

Journal of Social Theory in Art Education

This article sketches philosophical concepts of affect and event within the canvas of lived experience in a university art education teacher preparation course. We claim that by embracing architectural and metaphorically marginal spaces the course manifested transformative experiences for students, instructors, and community. We position and celebrate the often marginalized spaces of art education as potential sites of becoming through curricular rich environments and as thresholds of event for the educator of art within the community at large. Specifically, we describe the deconstructed space of the “classroom,” the curricular arc of learning, and the occurrence of an unplanned, emergent, student …

Patient-Specific Inference Of Average Glucose From Glycated Hemoglobin, Roy Malka, David M. Nathan, John M. Higgins

Biology and Medicine Through Mathematics Conference

No abstract provided.

Determinants Of The Efficacy Of Hiv Latency Reversing Agents And Implications For Drug And Treatment Design, Ruian Ke

Biology and Medicine Through Mathematics Conference

No abstract provided.

A Multi-Level Model Of Synergistic T Cell Activation, Anna Konstorum, Anthony Vella, Reinhard C. Laubenbacher

Biology and Medicine Through Mathematics Conference

No abstract provided.

Anticipating Elimination Of Mosquito-Borne Diseases, Suzanne M. O'Regan, Jonathan Lillie, John M. Drake

Biology and Medicine Through Mathematics Conference

No abstract provided.

The Influence Of Temperature Variation On Dengue Fever: A Model-Based Investigation, Michael A. Robert, Paula D. Weber, Rebecca C. Christofferson, Christopher N. Mores, Helen J. Wearing

Biology and Medicine Through Mathematics Conference

No abstract provided.