Digital Commons Network^™

Acute Effects Of Electronic Nicotine Delivery System Liquid Nicotine Form And Sweetener Among People Who Use Inhaled Tobacco Products, Mary A. Carrico

Theses and Dissertations

Research on tobacco product standards to limit the abuse liability of electronic nicotine delivery systems (ENDS) has primarily focused on nicotine concentration and flavor. Other liquid characteristics, such as protonated nicotine ratio and sweetener additives, are less understood but may serve as regulatory targets. This clinical laboratory study examined the effects of ENDS protonated nicotine ratio and sweetener presence on nicotine delivery, use behavior, and subjective effects.

Thirteen participants completed four sessions that varied by protonated nicotine ratio (0:100 vs. 40:60 freebase to monoprotonated nicotine) and sweetener status (sweetened with 3 mg/mL ethyl maltol vs. unsweetened). Each session included a …

Doves And Symbiotic Bacteria: Whole-Genome Assembly And Phylogenomic Inference, Mariam Topchyan

Theses and Dissertations

Part 1

I examined a potential source of variation in phylogenetic inference, investigating whether handling single-nucleotide polymorphisms (SNPs) through different methods during reference-guided genome assembly of pigeon and dove species impacts phylogenetic reconstruction and inference. Specifically, I created a custom consensus base calling tool that handles allelic variation through two different approaches, either ignoring heterozygous sites by denoting them as an ambiguous character “N,” or using a pseudo-random choice between supported base variants. Through reference-guided assembly of 108 dove species, I generated two datasets of alignments using both consensus calling methods, created two trees from both datasets via supermatrix and …

Improving Telehealth Education And Training: A Needs Assessment Of Healthcare Providers And Trainees, Conor Duffy

Theses and Dissertations

Telehealth utilization has increased dramatically since the start of the COVID-19 pandemic. There are many positive impacts of this shift to telehealth, including increasing access to care and reducing patient burden. However, healthcare providers report several consistent challenges that limit their ability to deliver telehealth, such as effective communication, technological difficulties, and difficulty performing physical examinations. To address these concerns, it is necessary to integrate telehealth education and training into healthcare professional training programs and healthcare systems. While many institutions have implemented telehealth education and training since the start of the pandemic, there is little consistency in the content, duration, …

Maternal Postpartum Depression And Affect Incongruence: Exploring Early Developmental Pathways To Emotion Dysregulation, Laura E. Boylan

Theses and Dissertations

Emotion dysregulation, or responses to emotions that do not meet the needs of the situational demands or social standards, has been noted as an early risk factor for later psychopathology. Maternal postpartum depression (PPD) has been shown to increase children’s risk of developing maladaptive emotion regulation (ER) strategies. One potential mechanism linking PPD to these outcomes is affect incongruence, or a mismatch between biological and emotional responses to stress. While such incongruence may be adaptive in older individuals who consciously mask their internal stress (e.g., suppressing biological arousal while appearing calms), it may be maladaptive in infants and young children …

Mutual Realm, Tyna Ontko

Theses and Dissertations

mutual realm is a container for my many meandering thoughts on speculative futures, present-day-truth-telling, and the everyday construction of histories. Informed by systems of belief and power that humans have created to grapple with the unknown, the writing illustrates how storytelling builds worlds, assigning value to things by inscribing them with desire. In parts fact and fantasy, sculpture is considered as a form of sorting, making known the ways in which my experience of living between publics whose methods for demarcating boundaries, communally agreed upon superstitions, and algorithms for assigning meaning have marked my unconscious and affect my daily trajectories. …

Strange World, Brooklin Grantz

Theses and Dissertations

My thesis work explores the intersections of masculine and feminine labors as they relate to the structure of domestic space. I utilize materials and processes akin to craft, construction and architecture, and domicile upkeep, noting what is shared between gendered and polarized fields while exploring uses of the grid in various instances as a structure to adhere to and antagonize: a binary. Highlighting the queerness and fluidity of materials and the similarities between seemingly disparate processes, I relate to the objects I make and alter through my own gender fluidity. I do not see masculinity or femininity as autonomous: the …

Imagine Being Free: Decolonizing Actor Training In University And Conservatory Programs, Hope R. Ward

Theses and Dissertations

While blackface is considered the first proper form of American theater, I want to consider how the auction of enslaved Africans functioned as the original form of American theater. The auction block acted as a kind of interactive performance in which enslaved bodies were surveilled and evaluated as a kind of spectacle. In many ways, the auction block inaugurated White viewing practices of the Black body that reverberated in the day-to-day practices of slavery and subsequent visual media like the refashioning of blackface minstrel tropes within contemporary entertainment (plays, television, film). In Imagine Being Free: Decolonizing Actor Training in University …

Bioremediation In The Chesapeake Bay: Anthropogenic Ocean Acidification Mitigation In An Interactive Multi-Trophic Ecosystem, Cree Taylor

AUCTUS: The Journal of Undergraduate Research and Creative Scholarship

Zostera marina is a species of marine macrophyte that has been largely prioritized in past submerged aquatic vegetation (SAV) restoration initiatives within the Chesapeake Bay because of its 1) previous abundance, 2) demonstrated carbon burial abilities, and 3) observed ability for meadows of Z. marina to ameliorate low pH conditions. Due to the temperature variability and poor water quality in the Chesapeake Bay, Z. Marina beds have experienced several die-off events with limited capacity for regrowth. Ruppia maritima, an opportunistic species with tolerance to temperature shifts within the bay, may have the potential to maintain SAV coverage in areas …

Dynamic Expression Of Transposable Elements Contributes To Molecular And Behavioral Adaptations To Cocaine, Gabriella M. Silva

Theses and Dissertations

The comorbidity of substance use disorder (SUDs) — specifically cocaine use disorder (CUD)— with anxiety and social deficit disorders complicates effective long-term treatment. More research is needed to understand the brain-region and cell type-specific mechanisms underlying these disorders to better address treatment gaps. This dissertation investigates the role of the transcription factor of interest ZFP189, a Krüppel-associated box (KRAB) zinc finger protein (ZFP), in governing pro-social behavior and the development of CUD across interconnected brain regions. We show that ZFP189 directly regulates transposable elements (TEs), repetitive DNA sequences capable of mobilizing and reinserting into the genome, which leads to subsequent …

Physiological Responses And Plant Trait Interactions With Coastal Dune And Swale Development, Emily C. Riffe

Theses and Dissertations

Vegetation is crucial to coastal ecosystems experiencing climate change and storm-induced disturbance. Plant species interact with the physical environment and are considered “ecosystem engineers.” Sand dunes and barrier islands are at the forefront of climate change impacts as they are barriers that protect landward habitat and communities. Nature based solutions are utilized to protect infrastructure on developed coastlines. Understanding how dominant plant species interact with and alter the physical environment enhances our understanding of the mechanisms of change and consequences to the system. The goal of this research was to quantify physiological and functional trait differences in dominant species found …

Review: The Shape Of Power: Stories Of Race And American Sculpture, Jiaying (Gloria) Dai

Art Inquiries

The Shape of Power: Stories of Race and American Sculpture, co-curated by Karen Lemmey,

Tobias Wofford, and Grace Yasumura, ambitiously asked: How does American sculpture

intersect with the history of race in the United States?1 Positioning itself as the first major survey

exhibition of American sculpture in decades, this exhibition featured 82 sculptures created by 70

artists spanning from 1792 to 2023.

Non-Enzymatic Glycoxidation Reports On Society, Environment And Biology To Inform On Enduring Cancer Disparities, Fariha Imtiaz, Boxiao Ding, Bradley A. Krisanits, Katherine A. Walter, Lindsay L. Peterson, Susan Steck, Marvella E. Ford, Gayenell Magwood, Victoria J. Findlay, David Turner

Annual Research Day

Background: Despite advancements in cancer treatment, disparities in incidence and mortality persist among different races and ethnicities. These disparities are influenced by an intricate interplay between environmental, biological and social factors that underscore the need for a multifaceted approach to understand this relationship. Objective/Aim: To show that advanced glycation end products (AGEs) due to non-enzymatic glycoxidation represent informative and functional markers that report on the combined impact of environmental, biological and social factors on enduring cancer disparities. Methods: To address this objective, a more holistic approach to disparities research was used ranging from basic research, population-based investigations, preclinical and clinical …

Post Opera, Suzy Lykins

Theses and Dissertations

Post Opera is a spell and work of embodied research which explores medicalization, desire, labor, and belief. Through methods of performance, sound, text and installation, I facilitate encounters of trans-femininity, spiritual power and connectivity. An opening wound, a set of psychic scalpels, sutures and bandages prepare this text for your consumption, take care to digest her well.

Exploring The Role Of Transcription Factor Nrf1 In Autophagy And Inhibiting The Nrf1 Bounce-Back Response, Madison A. Ward

Theses and Dissertations

Cells exposed to proteotoxic stress invoke adaptive responses aimed at restoring proteostasis. Our previous studies have established a firm role for the transcription factor Nuclear factor-erythroid derived-2-related factor-1 (Nrf1) in responding to proteotoxic stress elicited by inhibition of cellular proteasome. Following proteasome inhibition, Nrf1 mediates the synthesis of new proteasomes, thus enabling cells to mitigate proteotoxic stress. Here, we report that under similar circumstances, multiple components of the autophagy-lysosomal pathway (ALP) were transcriptionally upregulated in an Nrf1-dependent fashion, thus providing the cells with an additional route to cope with proteasome insufficiency. In response to proteasome inhibitors, Nrf1-deficient cells exhibited profound …

What Are The Genetic Influences Of Alcohol Sensitivity And Alcohol Metabolism? A Narrative Review Of Human Studies, Erwin Murray, Karen G. Chartier Phd, Msw

Undergraduate Research Posters

Background: Alcohol sensitivity and alcohol metabolism are two phenotypes associated with alcohol use disorder (AUD) influenced by genetics. This narrative review aims to answer “What are the genetic influences of alcohol sensitivity and alcohol metabolism?” to gain a better understanding of the genetic aspects of AUD.

Methods: A database search was performed in PubMed. Empirical human genetic studies published between 2009 and 2025 that focused on alcohol sensitivity (measured by the Self-Rating of the Effects of Alcohol scale) or alcohol-metabolizing genes were eligible for review. Studies were screened to determine inclusion for the current review.

Findings: Fifteen …

Using Test-Crosses To Investigate Mendelian Inheritance Patterns In Brassica Rapa, Jacob Batson, Amy Nguyen, Ethan Do

Undergraduate Research Posters

The Wisconsin Fast Plants, Brassica rapa, are an ideal model organism for exploring patterns of inheritance and phenotype expression due to their rapid life cycle and observable traits. In this experiment, we examined the controlled crosses between the two phenotypes, anthocyanin-producing (ACN) and anthocyaninless (ANL), to observe allele inheritance. We observed phenotypes in the F1 generation to see if it followed the Mendelian inheritance patterns of homozygous dominant. By planting and watering the plants for two weeks, we tracked their growth and traits of ACN and ANL phenotypes. We used honey bees to cross pollinate the parent ACN and ANL …

Effects Of Gas Exchange Rates Between C3 And C4 Plant Species, Samantha L. Moya, Mollie Ouk, Aisha Goni

Undergraduate Research Posters

Spinach and basil (C3 plants) were compared with corn (C4 plant) to

explore how photosynthetic pathways affect CO2 consumption. Corn

seeds were obtained and planted in Miracle-Gro® soil to be observed

over the duration of a month. Leaves (starting from 3,4, and 10) from

each plant were then taken and placed into a bottle and closed off

with a CO2 gas probe that measured levels of carbon dioxide in ppm.

Spinach and basil leaves were used as a comparison for measuring

the rate of CO2 concentration. This is done to compare the gas

exchange rates between C3 and C4 plants. …

Sex-Specific Regulation Of Mash And Hepatocellular Carcinoma: A Role Of Sphk2 In Females, Bria Pierre

Theses and Dissertations

Metabolic Dysfunction-Associated Fatty Liver Disease (MAFLD) is characterized by excessive fat accumulation in the liver, with metabolic dysfunction-associated steatohepatitis (MASH) representing a more severe form that can progress to cirrhosis or hepatocellular carcinoma (HCC). The sphingosine kinases and the S1P signaling pathway are key regulators of MASH and HCC progression. While much is known about the mechanisms contributing to MASH-HCC in males, less is known for females. Nevertheless, we previously identified sphingosine Kinase 2 (SphK2) to protect females from MASH-driven HCC. This study first performed differential gene expression analysis on human liver cohorts to examine sex-specific differences in gene expression …

The Diseased Extracellular Matrix In Chronic Obstructive Pulmonary Disease Modeling, Leigh-Ann M. Antczak

Theses and Dissertations

Chronic obstructive pulmonary disease (COPD) is currently the fourth leading cause of death in the world, with an increasing prevalence as the world’s population ages. In the distal regions of the lungs, COPD is characterized by progressive emphysema due to an imbalance of proteases that continuously degrade essential extracellular matrix (ECM) proteins within the alveolar walls, causing fatal tissue impairment. This dissertation examines the unique properties of this diseased lung ECM in two parts: (1) As a key constituent in inflammatory signaling that leads to increased inflammation and progression of COPD, and (2) as a tool in three-dimensional (3D) in …

Wiring Attachment, Alexander K. Bacon

Theses and Dissertations

This thesis began with a question: can diagrams create understanding, or only reflect it? Through paintings that layer automotive wiring schematics with family photographs, I explore how the human attachment system resists easy mapping. Using digital tools like Photoshop and scanning, alongside analog methods such as papier-collé and photo transfer, I examine how systems built for clarity—like wiring diagrams—contrast with the layered, often messy realities of human relationships. Drawing from attachment theory’s empirical foundation, rather than the metaphorical leanings of psychoanalysis, my work investigates how emotional bonds form, falter, and repair over time. Material loss and layering are not flaws, …

Smear Layer Removal By Irrigation Adjuncts: A Scanning Electron Microscopy Study, Triet D. Vuong

Theses and Dissertations

Purpose: This study aimed to compare the intracanal smear layer removal ability of two adjunctive irrigation modalities: the AdvErL EVO laser, the GentleWave^® Procedure, and their combination, after minimally invasive canal instrumentation.

Methods: Forty molars were accessed, the mesiobuccal canals of maxillary and mandibular molars were prepared up to apical size 25.04 using rotary instrumentation, then irrigated using: conventional needle irrigation (CNI), laser-assisted irrigation (LAI), the GentleWave^® Procedure (GWP), and LAI followed by GWP (LAI+GWP). The teeth were then decoronated and split longitudinally. One-half of each root was selected for preparation and analysis under scanning electron microscope. Blinded …

The Bacteriostatic, Regenerative, And Immunomodulatory Properties Of Extracellular Matrix Particles For Lung Injury, Keera P. Rhoads

Theses and Dissertations

Acute respiratory distress syndrome (ARDS) is a prevalent, life-threatening lung condition, affecting nearly 200,000 Americans annually, with a 40% international mortality rate. There is no cure for ARDS, and current pharmacological treatments have limited effectiveness. Symptoms can be mitigated with mechanical ventilation, though this often leads to ventilator-induced lung injuries (VILI) and puts critically ill patients at risk of infections, including ventilator-associated pneumonia (VAP). A promising therapeutic is the extracellular matrix (ECM), a complex network of structural proteins and bioactive molecules that has been shown to have anti-inflammatory properties and prevent fibrosis. We aim to utilize the regenerative and immunomodulatory …

Impact Of Substrates And Deposition Time On Bacteria Associated With Saliva Samples And Its Implication On A Bacterial Signature-Based Body Fluid Identification Method, Olisdey Galano Cruz

Master of Science in Forensic Science Directed Research Projects

Traditional serological methods for body fluid detection are limited by poor specificity and sensitivity, lacking confirmatory capability and thereby increasing the risk of false positives that may compromise the integrity of forensic investigations. While molecular methods such as mRNA, miRNA, and DNA methylation offer improved specificity, they are often hindered by marker instability and operational complexity in routine forensic workflows. Bacterial signature profiling has emerged as a promising alternative, offering enhanced stability, abundance, and compatibility with existing DNA workflows. However, its forensic implementation requires further validation. This study evaluated the effects of substrate type and storage time on the salivary …

Qualitative Method Validation Of Acid/Neutral Drugs Carisoprodol, Meprobamate, And Naproxen Using Liquid Chromatography-Tandem Mass Spectrometry, Kate O. Martin

Master of Science in Forensic Science Directed Research Projects

Meprobamate, carisoprodol, and naproxen are acidic/neutral drugs widely prescribed for muscle relaxation and inflammatory conditions, with frequent detection in forensic toxicology casework due to extensive clinical use. A method to qualitatively evaluate these compounds was developed to unify acid/neutral drug screening. This study incorporated blank blood, antemortem blood, postmortem blood, urine, and liver homogenate as representative biological matrices. Method validation was conducted in accordance with ANSI/ASB Standard 036 for Forensic Toxicology, encompassing key parameters including limit of detection (LOD), endogenous interferences, potential interferences, carryover, processed sample stability, and ionization suppression/enhancement. The validation was performed using the internal standards carisoprodol-D7, meprobamate-D7, …

Novel Time-Since-Deposition Signatures For Canine Blood; Implications For Animal Cruelty Investigations, Alysia M. Townsley

Master of Science in Forensic Science Directed Research Projects

Dogfighting is a felony in all 50 states and is often tied to other organized crime activities including gambling, narcotics, and weapons trafficking. Because of the clandestine nature of dogfighting crimes, it may be necessary to determine the age of blood samples from suspected events, not just the source. In this study, flow cytometry was used to identify cellular autofluorescence signatures for estimating TSD (time-since-deposition) in canine blood deposits up to 6 months old. To accomplish this, blood was taken from canines of various chronological ages and breeds, then deposited to create TSD samples ranging between 1 day and 180 …

Development Of Novel Signatures For Determination Of Cell Types In Blood And Saliva Mixtures, Caitlin Lydon

Master of Science in Forensic Science Directed Research Projects

Determination of tissue source and the time at which a biological material is transferred from its source to another surface, known as the time-since-deposition (TSD), would assist forensic analysts by providing crucial context for DNA profiles generated from biological evidence. Many methods for cell identification have been explored using biomolecule markers such as mRNA, miRNA, and differential DNA methylation. Forensic laboratories have also developed methods for TSD estimation that focus on degradation of biomolecules. Biomolecules such as mRNA or miRNA degrade at different rates due to some being more stable than others. The ratio of stable degrading biomolecules, therefore, can …

Exploring The Stem Career Identity Development Of Black Women Across Their Lifespan, Veronica Hurd

Theses and Dissertations

Although STEM is the fastest-growing career sector, Black women are grossly underrepresented as they account for 2.5% of the workforce. Research highlights that this underrepresentation is due to racialized structures in K-12, postsecondary, and career settings that restrict Black girls’ and women’s STEM opportunities. While macrosystems such as hegemonic ideologies, attitudes, and social conditions shape Black girls’ and women’s opportunities in STEM, they continue to persist and achieve their career goals. To explore these barriers and Black women’s persistence in this industry, this study draws from the autobiographical memories of 10 Black women in the field or formerly in the …

Expanding The Performance And Functionality Of Air-Jet Dry Powder Inhalers Across Multiple Applications, Ghali H. Aladwani

Theses and Dissertations

This dissertation aims to expand the performance and functionality of air-jet dry powder inhaler (DPI) applications through the development of a next-generation, mesh-nebulizer-based, and scalable spray dryer for the production of pharmaceutical aerosols, along with new spray-dried formulation production methods, novel formulations, and new air-jet DPI components for aerosol delivery to both infants and adults. The first aim focused on the development of a custom small-particle spray drying system capable of integrating vibrating mesh nebulizer technologies in various configurations for the production of excipient enhanced growth (EEG) formulations. This flexible system enabled efficient production of both small- and large-particle powders …

Uncovering Graduate Student Achievement At Virginia Commonwealth University: An Exploration Of Academic Advising & Administrative Processes, Kenzel Hill, Elizabeth Howley, Madeline Schutt, Michelle Blackwell

Doctor of Education Capstones

Virginia Commonwealth University's (VCU) Graduate School submitted a Request for Partnership, with the problem being a lack of consistency and formal advising standards for graduate students across various academic units at VCU. As a result, the Graduate School observed an impact on student success, including retention and graduation of graduate students. A capstone team was constructed to determine, through focus group interviews, surveying, and document analysis what the best practices for graduate student advising are and what the administrative processes and challenges that program directors, support staff, and faculty face in administering their graduate programs and advising. Findings suggest that …

Innovative Secondary School Scheduling Practices To Positively Impact Student Outcomes In West Point Public Schools, Jessica Burdette, Sydney B. Lilly, Mark A. Strickler

Doctor of Education Capstones

Utilizing the principles of improvement science through a critical pragmatic approach, this study aimed to promote West Point Public School (WPPS) student success through researching secondary school scheduling practices. The primary theory of change for this study stated that if instructional time is maximized, all WPPS students will be given the opportunity to experience academic success and increase achievement. Three key research questions emerged as we analyzed the problem of practice: How can WPPS structure a secondary school day schedule to ensure every student has the best opportunity to succeed within the system? How can WPPS incorporate research-based strategies for …