Digital Commons Network^™

The Effects Of Amixicile, A Pyruvate Ferredoxin Oxidoreductase Inhibitor, On Oral Treponemes, Lucas A. Reed

Theses and Dissertations

Periodontal disease (PD) is a polymicrobial infection characterized by inflammation of the gingiva, alveolar bone resorption, and tooth loss (edentulism). Treponema denticola along with Porphyromonas gingivalis and Tannerella forsythia are among the “Red Complex” and are main etiological agents in PD. Treponemes are a member of the Spirochaeta phylum and are obligate anaerobes, that express pyruvate ferredoxin oxidoreductase (PFOR). The enzyme catalyzes the oxidation of pyruvate to acetyl-CoA and reduced ferredoxin. Amixicile is a novel bacteriostatic derivative of nitazoxanide and an inhibitor of PFOR. In light of the fact that Treponemes express PFOR, this study was conducted to investigate the …

Functional Consequences Of Mtdna Methylation On Mitochondrial Transcription Factor Binding And Transcription Initiation, Elliot N. Burton

Theses and Dissertations

The role of cytosine modifications on nuclear transcription has been well characterized, but the function of DNA methylation in the mitochondrial genome has not been determined. Previous studies conducted by the Taylor laboratory have shown overexpression of the mitochondrial isoform of DNMT1 leads to strand-specific changes in gene expression. Here, we show that increased mtDNMT1 expression leads to an increase in the polycistronic transcript encoding the ND1 and Cox1 sequences. In order to understand the mechanistic basis of these changes, we investigated the effects of CpG methylation in the heavy strand promoter on transcription initiation and TFAM binding. Methylation was …

Supporting Teen Leaders: Validation Of The I Drive Smart Survey, Cynthia M. George

Theses and Dissertations

Recent policy change allows states to spend federal dollars directly on teen-led driver safety efforts and requires regular evaluations of effectiveness. There are currently no standardized instruments to measure change in teen driving behavior relevant to teen leaders. This study serves the Tennessee Department of Safety and Homeland Security, Tennessee Highway Safety Office and their network of teen leaders to empirically test and refine the I Drive Smart survey developed by partners and grounded in the Theory of Planned Behavior (TPB). The survey is designed to be administered by teen leaders to their peers and produce data relevant for use …

Methcathinone Analogue Activity At The Human Serotonin Transporter, William Drake Varn

Theses and Dissertations

In the last few years, there has been continued concern about synthetic drug abuse in both the United States and worldwide. Small adjustments in drug compound structure often allow synthetic drug makers to manufacture a legal product that can produce the same highs as illegal counterparts. Unfortunately, this is happening faster than the government can outlaw the drug compounds, and a wide variety of synthetics are now appearing on the street. This study evaluated the effects on the human serotonin transporter of six different 4-para substituted methcathinone compounds. Using a Xenopus oocyte model, the efficacy of each MCAT analogue …

Collective Cadence, Alicia Dietz

Theses and Dissertations

Collective Cadence

Stories that start at birth,

end with death,

and include everything in-between.

A Medal of Honor recipient,

A mistake.

Morning coffee;

a late night call.

Whispered first names;

an off-key chorus;

deafening explosions;

silence.

One child playing;

another lifeless.

On the battlefield,

concealment is a survival skill.

Off that battlefield,

transparency enables survival.

Stories that shape the fabric of our experience

The threads that bind us

Through the telling of each,

we are united as one.

In the telling of the collective,

We find our own.

These are our stories.

This is our story.

Boomerang Studio: Community Design For Action, Jessica Walton

Theses and Dissertations

An arts education space, focusing on concepts of design thinking aimed at providing low income middle and high school teens of color the opportunity to learn about interior design, architecture and crafts. Ultimately, Boomerang Studio hopes to engage students in the world of design as a means of creating greater diversity within these disciplines.

No Woman Is An Island, Adele Ball

Theses and Dissertations

We have gathered the following pages to archive our time here in Richmond, Virginia. We have been here for two years, growing slowly, moving when needed to new anchorholds to avoid detection or arrest. We scrutinize the urban environment like modern archeologists. We collect stories and speculate about new uses of old things. It is imperative to be resourceful here, and we do so out of necessity but also in the spirit of practice. These pages were made en route, each an exploration of the tools at hand when on the move. The method of creation is just as important …

A (Dis)Assemblage Of The Gallery-Growlery, Levester R. Williams

Theses and Dissertations

A (dis)Assemblage of the Gallery-Growlery exhibition and writing presents itself as a site of a morphological exploration of language, sound, and objects in tandem with the irreducibly venting black expression. Venting, the black expression never seeks wholeness within objects or language itself for it is a thing-in-itself. Its presence affords critical reception to a residue of delimiting forms. All growls eschew verbal objects for the manifestation of pure phonetics. A growl in a gallery is the growl. The growl resounds through the physicality of the objects and gallery. Also, it unwinds the object-among-objects as the phono-present stretches the discursive …

Monitor, Shana Hoehn

Theses and Dissertations

Descriptions of a scene interwoven with thoughts on how aesthetics sets behavioral expectations, observation as proof, views from a single perspective, casting of characters, spaces that evoke complicated histories, and my own history.

Truth And Doubt In Representation, Mathew Gasparek

Theses and Dissertations

My work deals with the connection between the act of representation and conceptions of truth in the contemporary image-mediated social environment. This thesis details elements of my paintings in exchange with methods and ideas from artists and writers in order to position my aesthetic and conceptual sensibilities within a historical and contemporary framework.

A Theoretical Simulation Of The Settling Of Proppants In A Hydraulic Fracturing Process, Nisreen Alseamr

Theses and Dissertations

Hydraulic fracturing is a process for the extraction of hydrocarbons from underground formations. It involves pumping a specialized fluid into the wellbore under high pressures to form and support fractures in the rock. Fracturing stimulates the well to increase the production of oil and the natural gas which are the pillars of the energy economy. Key to this process is the use of proppants, which are solid materials used to keep the fractures open. Understanding the transport of proppant particles through a fluid is important to improve the efficiency and reduce environmental impact of fracturing. An increase of the settling …

Antithetical Commentaries On X, Y And The Disruption Of Being, Eva Rocha

Theses and Dissertations

Through discursive essays and poetic narrative, Antithetical Commentaries on X, Y and the Disruption of Being explores the tenuous relationship between modes of measurement and the struggle for human relevance in the post-contemporary digital age. In the introductory essay, “Not the Feather, but the Bird”, I give an overview of the inherent problems of object-oriented ontology, and how it relates to aesthetics and social issues of our times. In the Developmental Overview, I detail how I developed my installation approach and techniques, particularly with regard to the three-way dynamic of the artist:work:viewer relationship and how it can encourage …

Identifying Misconceptions Associated With Inaccurate Survey Reporting In The Combined Use Of Caffeine And Alcohol, Kathryn Polak

Theses and Dissertations

Research on college student use of caffeine combined with alcohol (CAC) and public health concern over such use has been hampered by the absence of psychometrically sound measures of caffeine and CAC use. The present study examined agreement between survey (CAS) and interview (TLFB) methods for collecting data on caffeine, alcohol and CAC use. Participants were N=50 college students randomized to complete CAS followed by TLFB or the reverse. Qualitative follow-up interviews with N=15 participants were used to identify factors contributing to CAS-TLFB discrepancies. Responses varied by method of administration, with largest discrepancy magnitudes found for CAC, followed …

Holistic Products: Designing With Time, Gifts, And Ritual, Jeremy P. Zietz

Theses and Dissertations

The notion of “you are what you buy” is an updated adage from “you are what you eat”. It makes a connection between our everyday objects and their effect on our lived experience. Looking at our relationships with our things as a type of contract, we must be intentional to shape these object contracts for our own good and health. Instead of our society’s design talents being put toward a consumerist agenda, designers must direct research and development which addresses the effects of our products holistically.

Various concepts have emerged in my creative practice which demand a deeper research and …

Synthetic Landscapes, Benjamin Thomas Jordan

Theses and Dissertations

My work explores the complex social geography of modern society and the intricate relationship between mankind and the environment. Through this work I explore the past and present lineage of manifest destiny, from its beginnings in Europe to western expansion in America, to forms it has takes in contemporary America. These ceramic forms serve as the conceptual grounds to explore the romanticizing of the western landscape especially from an individual and group perspective. I simultaneously celebrate the history of the pastoral life while questioning the authenticity, and motivations of that lifestyle, and use this platform as a jumping off point …

Toward Epistemological Diversity In Stem-H Grantmaking: Grantors’ And Grantees’ Perspectives On Funding Indigenous Research, Programming, And Evaluation, Jessica C. Venable

Theses and Dissertations

Mainstream institutions have, historically, dismissed Indigenous worldviews, knowledges, and research approaches (Bowman-Farrell, 2015; Harrington & Pavel, 2013). However, in recent years, a literature has emerged articulating Indigenous research methodologies (IRMs), and their distinctiveness from Western, Eurocentric perspectives on inquiry (Denzin, Lincoln, & Smith, 2008; Kovach, 2009; Smith, 1999 & 2012; Wilson, 2008). This has coincided with increased need for IRM scholars and practitioners to secure extramural funds to support their activities. But questions remain as to how the U.S. federal grant making enterprise has accommodated Indigenous frameworks. This research explores synergies in the ways that grantees, grant makers, and other …

Radioprotective Cerium Oxide Nanoparticles: Molecular Imaging Investigations Of Conps’ Pharmacokinetics, Efficacy, And Mechanisms Of Action, Philip R. Mcdonagh Iii

Theses and Dissertations

Cerium oxide nanoparticles (CONPs) are being investigated for several anti-oxidant applications in medicine. One of their most promising applications is as a radioprotective drug, an area of research in need due to the severe side effects from radiation therapy. In this work, the potential of CONPs as a radioprotective drug is examined using four criteria: favorable biodistribution/pharmacokinetics, low toxicity, ability to protect normal tissue from radiation damage, and lack of protection of tumor. The mechanisms of action of CONPs are also studied. Biodistribution was determined in radiolabeled CONPs with surface coatings including citrate, dextran T10-amine (DT10-NH₂), dextran T10-polyethylene …

Modern Art Of Pakistan: Lahore Art Circle 1947-1957, Samina Iqbal

Theses and Dissertations

This dissertation focuses on the modern art of Pakistan from 1947-57, more specifically, the role of six important artists who founded the Lahore Art Circle (LAC) in 1952. The group played a pivotal role in the formulation of modernism in Pakistan after its establishment as an Islamic Republic. Framed within postcolonial theories and criticism, this study will address the role of modern art in developing new artistic sensibilities in the nation of Pakistan. In order to understand the context of LAC’s framing of “modernism” and “nationalism” in terms of specific historic and hybrid nexus,my research will provide an investigation of …

Adducins Are Negative Regulators Of Migration And Invasion Of Normal Lung Epithelial Cells And Lung Cancer Cells, Parth Hitenbhai Amin, Parth Amin

Theses and Dissertations

Cell migration is an important component of many physiological and pathological processes such as tissue and organ morphogenesis during development, wound healing, inflammatory immune response, and tumor metastasis. The actin cytoskeleton is the basic engine driving cell migration. In the present study, we elucidate the role of an important actin interacting proteins, Adducins, in motility of normal lung epithelium and lung cancer cells. Adducins are the family of cytoskeleton protein capping the fast growing end and facilitating the bundling of actin filaments. Adducins are encoded by the three closely related genes namely alpha (ADD1), beta (ADD2) and gamma (ADD3) Adducin. …

Irreversible Zinc Block Of The Swelling-Activated Chloride Current In Di Tnc1 Astrocytes, Noah Belkhayat

Theses and Dissertations

The swelling-activated chloride current, commonly referred to as ICl,swell, is an outwardly-rectifying anion current that plays an important role in cell volume regulation, among other capacities. Despite several decades of research, the molecular identity of the channel responsible for this chloride current remains controversial. Recent indications that key endogenous sulfhydryl groups are capable of modifying the current led us to assess the effects of several divalent cations, including zinc, on ICl,swell. Zinc is known to tightly associate with sulfhydryl groups such as in zinc finger proteins. We found that extracellular zinc irreversibly inhibited ICl,swell at a site downstream in the …

Cancer Health Literacy And Hospitalization In The First Five Years Following A Cancer Diagnosis, Laura A. Cartwright

Theses and Dissertations

BACKGROUND: The field of health literacy research has been focused recently on developing more accurate measurement tools and understanding the relationship between health literacy and health outcomes. Individuals with lower levels of health literacy have worse health outcomes, including hospitalization rates, compared to those with adequate health literacy. This relationship has yet to be examined in the cancer patient population, although significant relationships between health literacy and cancer knowledge, screening behavior and quality of life have been found. This study is the first to examine the relationship between health literacy and hospitalization rates in a cancer patient population, and the …

Student Engagement In Undergraduate Social Work Education Among “At-Risk” Students, Ananda Newmark

Theses and Dissertations

College student engagement is an important factor that contributes to student success. This study is one of the first to explore student engagement in undergraduate social work education by examining engagement levels among at-risk social work students. In this study, two types of at-risk student groups were studied: First Generation College Students (FGCS) and transfer students. A cross sectional research design was used. Secondary analysis was performed on data gathered by the National Survey of Student Engagement (NSSE) from five accredited, Bachelor of Social Work (BSW) programs in one southeast state. A sample of 135 BSW seniors were included in …

Exploring Visual Prevention: Developing Infographics As Effective Cervical Cancer Prevention For African American Women, Candace P. Parrish

Theses and Dissertations

The purpose of this dissertation is to explore the creation of a cervical cancer health prevention infographic, geared toward African American women ages 30 to 65, to be potentially used to raise health literacy and influence positive behaviors towards practicing prevention measures—ultimately preventing unnecessary deaths. This dissertation also produced a cervical cancer health prevention infographic prototype for African American Women ages 30 to 65 to be further tested and implemented within future health communication campaigns.

Cervical Cancer is both preventable and treatable (if diagnosed during early stages); therefore the amount of individuals dying from the cancer should be minimal. Still, …

Upper Thermal Limits Differ Among Component Species In A Host-Parasitoid-Hyperparasitoid System, Kanchan A. Joshi

Theses and Dissertations

Among the predicted impacts associated with global climate change, warming is of special interest because the rates of physiological processes are temperature-dependent. Insects and other ectotherms are likely to be affected due to their limited ability to control body temperature. In this study, I measured the tolerance to extreme high temperatures, i.e., critical thermal maximum (CT_max), of component species in a tri-trophic system, including an herbivore (Manduca sexta), a primary larval parasitoid (Cotesia congregata) and a hyperparasitoid (genus Silochalcis). For wild insects, the parasitoid had the lowest CT_max, the hyperparasitoid had …

In-House Fabrication Of Multi-Sized Polystyrene Microcarriers For Neural Stem Cell Expansion, Jessica Forrester

Theses and Dissertations

The clinical demand for neural stem cells drives the need for a reproducible method used to generate a large quantity of well-characterized cells to support regenerative therapies. Microcarriers are currently being used as a scaffold for stem cell culture to aid in this expansion process because they provide a larger surface area while also requiring fewer cell passages compared to monolayer culture. The main objective of this project was to investigate how human neural stem cell attachment, proliferation, and multipotency would respond to three-dimensional (3D) culture using peptide-coated microcarriers of varying diameters. In this study, multi-sized polystyrene microcarriers were fabricated …

Role Of The Exopolysaccharide Alginate In Adherence To And Inflammation Of Pulmonary Epithelial Cells, Brian E. Crossley

Theses and Dissertations

Pseudomonas aeruginosa (PA) infections in Cystic Fibrosis (CF) patients are not easily cleared due to the conversion from a nonmucoid to a mucoid phenotype. Alginate is an acetylated exopolysaccharide produced by mucoid PA that is responsible for increased resistance to antibiotics, host phagocytic killing, and propagating biofilm formation. Understanding the interaction between PA and host cells is critical to understanding chronic infection and inflammation in CF. In order to investigate this, we used A549 pulmonary epithelial cells and murine alveolar macrophages (MH-S) to examine host response to nonmucoid versus mucoid PA infection. Adhesion assays in A549 pulmonary epithelial cells revealed …

From Seed To Sky: Impacts Of Explosive Compounds On Vegetation Across Spatial And Developmental Scales, Stephen M. Via

Theses and Dissertations

Explosive compounds are broadly distributed across the globe as a result of nearly two centuries of munitions use in warfare and military activities. Two explosive compounds have seen disproportionate use; RDX (hexahydro-1,3,5-trinitro-1,3,5-triazine) and TNT (2-methyl- 1,3,5-trinitrobenzene), being the most commonly found explosives in the environment. The effects of explosives on biota have been studied in great detail; however, there is a general lack of understanding with regard to broader ecological impacts of these contaminants. My dissertation objective was to follow the impacts of explosive compounds on vegetation across scales. Impacts on vegetation at the species scale alter community composition via …

Effects Of Antidepressants On Human Mesenchymal Stem Cell Differentiation On Clinically Relevant Titanium Surfaces, Nancy B. Ayad

Theses and Dissertations

Selective Serotonin Reuptake Inhibitors (SSRIs) are the most frequently prescribed class of drugs worldwide and are implemented in the treatment of depression and other psychiatric disorders. SSRIs relieve depressive symptoms by modulating levels of the neurotransmitter serotonin in the brain. SSRIs block the function of the serotonin transporter, thereby increasing concentrations of extracellular serotonin. However, serotonin levels in the neurons of the brain only account for 5% while the remaining 95% is present outside the brain. Serotonin receptors and transporter are located on bone resident cells (mesenchymal stem cells (MSCs)), osteoblasts and osteoclasts, and serotonergic activity is believed to affect …

Plant Functional Trait And Hyperspectral Reflectance Responses To Comp B Exposure: Efficacy Of Plants As Landmine Detectors, Paul V. Manley Ii

Theses and Dissertations

At least 110 million landmines have been planted since the 1970s in about 70 nations, many of which remain in place today. Some risk of detection may be mitigated using currently available remote sensing techniques when vegetation is present. My study focused on using plants as phytosensors to detect buried explosives. I exposed three species representing different functional types (Cyperus esculentus (sedge), Ulmus alata (tree), Vitis labrusca (vine)) to 500 mg kg^-1 of Composition B (Comp B; 60/40 mixture of hexahydro-1,3,5-trinitro-1,3,5-triazine (RDX) and 2,4,6-trinitrotoluene (TNT)), a commonly used explosive mixture, and measured functional traits and reflectance over …

Expansion And Exclusion: A Case Study Of Gentrification In Church Hill, Kathryn S. Parkhurst

Theses and Dissertations

This thesis explores the gentrification process in Church Hill, one of the oldest neighborhoods in Richmond, Virginia. After World War II, Richmond residents knew Church Hill mostly for its crime rate and dilapidated housing. The white, middle-class flight to the suburbs left the remaining residents, mostly African American, to experience decades of disinvestment. Church Hill was considered a neighborhood to avoid for much of the late twentieth century. Yet, Church Hill is currently one of the most desired neighborhoods in Richmond, particularly for young professionals. This thesis seeks to explain the reasons why there has been such a dramatic change …