Digital Commons Network^™

The Role Of The Nucleosome Remodeling Factor Nurf In Inhibiting T And Natural Killer Cell Mediated Antitumor Immunity By Suppressing Tumor Antigenicity And Natural Cytotoxicity Receptor Co-Ligands, Kimberly Mayes

Theses and Dissertations

Tumor immunoediting is a dynamic process in which the immune response attacks tumor cells by detecting danger signals and tumor antigens. In order to survive, tumor cells develop mechanisms to avoid detection or destruction by the immune system. To counteract this, several strategies are being developed to enhance the antitumor immune response, including the depletion of immunosuppressive cells, enhancing the activation of antitumor immune cells and increasing tumor cell immunogenicity. These therapies have seen limited success individually, however, and it is likely that combination therapy with novel targets will be necessary to see reproducible beneficial responses. Epigenetic modifications are attractive …

Ethnic Racial Identity, Social Transactions In The Classroom, And Academic Outcomes, Krystal R. Thomas

Theses and Dissertations

Using a transactional framework, this study explored social relationships in the classroom as mediators of the association between ethnic-racial identity and academic outcomes. Participants were 101 5^th graders of diverse backgrounds who completed computer-based questionnaires about their friendships, ethnic-racial identity, and academic engagement. Teachers reported on closeness and conflict in the student-teacher relationship. Relationships in the expected direction were evident between the public regard and centrality dimensions of ethnic-racial identity and social relationships as well as with academic outcomes. Further, path analyses revealed that the relationship between the public regard and cognitive engagement was mediated by student-teacher closeness. Gender …

Design For Disassembly - A Circular Approach, Wajiha Pervez

Theses and Dissertations

As the world becomes increasingly aware of the need to better care for the environment, innovative business models are helping to counter the damage of the fast fashion system - a phenomenon in the fashion industry whereby production processes are expedited in order to get new trends to the market as quickly and cheaply as possible.

Designing products with a focus on their renewability can shift the product-consumer relationship. The closed loop concept of a “circular economy” is emerging as a viable and promising solution to the current linear business model.

This study explores the possibilities of a more mindful …

Community Gardens: Exploring Race, Racial Diversity And Social Capital In Urban Food Deserts, Jennifer F. Jettner

Theses and Dissertations

Study purpose. The study examined race and racial diversity in community gardens located in Southern urban food deserts, as well as the capacity of community gardens to generate social capital and promote social justice. Methods. A mixed-methods approach was used to describe characteristics of gardeners and community gardens located in urban food deserts, and test Social Capital Theory hypotheses. A convenience sample of 60 gardeners from 10 community gardens was obtained. Data was collected using surveys and semi-structured interviews. Analyses. Univariate and bivariate statistics were used to describe gardeners and gardens. Leader rationales for garden characteristics were analyzed using thematic …

Designing For Divorce: New Rituls And Artifacts For An Evolving World, Yang Soon Ju Ms.

Theses and Dissertations

Our interactions with objects build cultural codes, reflecting lifestyles, values, and identities beyond functional expectations. With open connectivity in the contemporary consumer environments, we have access to homogenized material cultures not only for daily activities but also for ceremonies and rituals to mark important events, such as birth, marriage, and death. What will happen to our cultural codes and diverse traditions when various cultural norms meet, exchange, clash, hybridize, and evolve?

In this research, globalized material cultures were investigated to discover metaphoric comparisons, to formulate conceptual frameworks, and to develop informed design, which can address evolving cultural conditions appropriately, in …

(Re)Mediating The Spirit: Evangelical Christian Young Adult Media, Tamara Watkins

Theses and Dissertations

"We are in the world, but not of the world," a maxim frequently spoken in evangelical Christian culture, provides insight into how these individuals view their relationship with secular culture. They presume to share the same temporal plane with secular culture, but do not participate in it. In this dissertation, I explore whether the division between evangelical Christian culture and secular culture is as clear as this aphorism implies. To facilitate this investigation, I examine media Christian content creators created for an American evangelical Christian young adult audience in the early twenty-first century, specifically focusing on novel-length fiction, comics and …

Socioecological Aspects Of Tobacco Use In College Populations, Michael D. Sawdey

Theses and Dissertations

Background: Although cigarette smoking is decreasing in the US and among college students, tobacco use remains a major public health problem. Specific socioecological gaps in the literature related to college tobacco use exist including: trends of tobacco use, combinations of polysubstance use, and availability of tobacco products around college campuses.

Objectives: The main goal of this research was the understand interrelationships that exist within a socioecological framework of college tobacco use. Specifically, this project aims to: 1) examine trends of tobacco and polytobacco use by gender and associations of polytobacco use and perceived peer tobacco use, 2) examine …

Optimization Of In Vitro Transcription/Translation Conditions For In Vitro Compartmentalization Studies And Synthesis Of 4-Fluorohistidine, Christine Ring

Theses and Dissertations

Genetic code expansion allows the incorporation of non-canonical amino acids with a variety of new functional groups: fluorescent amino acids,^1-3 azides,^4-6 alkynes,^5-10 and photocrosslinkers.^4,11,12 This incorporation requires the evolution of new tRNA/aminoacyl tRNA sythetase pairs. Traditionally screenings of novel tRNA/aminoacyl tRNA synthetase pairs have been done in vivo. While these in vivo screenings have proven robust, they are limited in multiple ways: non-canonical amino acids (ncAAs) must be nontoxic and bioavailable. Furthermore, library size is limited by transformation efficiency. Lastly, in vivo screenings require substantial amounts of the target ncAA, which is often not available …

Salvianolic Acid B For Pulmonary Delivery Towards Reversal Of Emphysema, Sneha Dhapare

Theses and Dissertations

A new pathobiologic hypothesis has recently emerged that the alveolar structural destruction and loss in emphysema are caused by the deficiency of vascular endothelial growth factor (VEGF). Therefore, this project hypothesized that such pathobiologic VEGF deficiency of emphysematous lungs can be recovered with a natural caffeic acid tetramer, salvianolic acid B (SalB), through activation of signal transducer and activator of transcription 3 (STAT3), so that emphysema can be reversed as a result of inhibition of induced cell death, stimulation of cell proliferation and migration, and promotion of stem cell recruitment to the lungs.

SalB was first shown to be potently …

Yi, Observational Documentary Aesthetics, And The Identity Politics Of Transcultural Migrancy, Jiacheng Xu 4159187

Theses and Dissertations

There is a moment in Edward Yang’s acclaimed film Yi Yi (2000) in which a young boy in a conversation with his father observes that he cannot see what his father sees and that his father cannot see what he sees, prompting two questions: “How can I know what you see?” and “Can we only know half of the truth?” Unable to provide adequate answers, his father instead offers his son a camera. Later in the film, the same boy presents his uncle with a picture he took of the back of his head. When asked why, the boy responds …

The Fractured Memory Of A Mind’S Eye, Russell G. White

Theses and Dissertations

The work I create is informed by questioning reality/identity, the fractalizing planes

of existence our essence occupies, and the artifacts of memory experience navigating

through space time. While existing in this realm of oversaturated media and neon

glow, I question the effects of pervasive data systems overloading or programming the

mental software we possess. My work includes humor as a means of exploring these

conventions while also displaying psychedelic surrealist imagery to help break away

from the conscious prison this existence births our concept apparatuses within.

A Window The Color Of Her Sunburn, Joana Stillwell

Theses and Dissertations

I use video and material fragments to investigate the collapse of virtual and physical spaces as memories, lived environments, and digital interfaces become overlaid and interchangeable. I am interested in the capacity for technology to propose alternative strategies in which to engage with the world as we continue to extend ourselves in new and enduring methods. Seemingly unremarkable fragments offer new potentials in questioning meaning, worth, and care within spaces of downtime, boredom, and play.

This document accompanies my thesis exhibition a window the color of her sunburn. It provides background information on selected fragments and residues from my own …

Relocations Of The 'Outraged Slave': Transatlantic Reform Conversations Through Douglass's Periodical Fiction, Nikki D. Fernandes

Theses and Dissertations

Through their editorial arrangements of African-American, Euro-American and European poetry, fiction and news, Frederick Douglass’s anti-slavery periodicals (The North Star and Frederick Douglass’ Paper) imagine a cosmopolitan discourse that predates the segregated realities of the antebellum United States. In spite of Southern blockades against the infiltration of Northern texts, Douglass’s material space uniquely capitalized on the limited restrictions of his reprinting culture to relocate the voice of the ‘outraged slave’ onto a global stage. From the poems of Phillis Wheatley and William Cowper to Charles Dickens’s Bleak House and Douglass’s own novella “The Heroic Slave,” this project considers how …

Below The Neck, Above The Knees, Desiree Dawn Kapler

Theses and Dissertations

My thesis explores the act of violation in the context of trauma and healing through the use of personal narratives and experimental film. My research allows personal storytelling to transform into a larger and more universal theme of generational trauma and dysfunction. Through a feminist lens, I challenge social norms of body autonomy for the sick and abused, capitalism’s social effects on the poor, and passed down maternal lessons from the women who are doing the best that they can with the lives and opportunities that they have been given.

This work is created in spite of the labels my …

Biochemical Analysis Of Putative Single-Stranded Nucleic Acid Binding Proteins In Porphyromonas Gingivalis, Steve H. Kokorelis

Theses and Dissertations

Proteins that bind to both DNA and RNA embody the ability to perform multiple functions by a single gene product. These nucleic acid binding proteins in prokaryotes can play a vital role in many cellular processes, including replication, transcription, gene expression, recombination, and repair, to name a few. Nucleic acid binding proteins have unique functional characteristics that stem from their structural attributes that have evolved in a widely-conserved manner. In Escherichia coli (E. coli), the highly-conserved histone-like protein, HU, which predominates as a heterodimer of HUα and HUβ, has been found to bind to both dsDNA and ssDNA. …

Nanocomposite Bioelectronics For Biopotential Enabled Prosthesis, Dong Sup Lee

Theses and Dissertations

Soft material-enabled electronics can demonstrate extreme mechanical flexibility and stretchability. Such compliant, comfortable electronics allow continuous, long-term measurement of biopotentials on the skin. Manufacturing of the stretchable electronic devices is enabled by the recent development combining materials transfer printing and microfabrication. However, the existing method using inorganic materials and multi-layered polymers requires long material preparation time and expensive processing cost due to the requirement of microfabrication tools and complicated transfer printing steps. Here, this study develops a new fabrication method of soft electronics via a micro-replica-molding technique, which allows fast production, multiple use, and low cost by avoiding microfabrication and …

Combining Noxa-Inducing Drugs With Abt-263 To Efficiently Increase Cell Death In Head And Neck Squamous Cell Carcinoma (Hnscc), Sung Woo Kim

Theses and Dissertations

Head and neck cancer is the sixth leading cancer worldwide. Head and neck squamous cell carcinoma (HNSCC) accounts for more than 90% of incident cases. Despite intense, multimodality treatment regimens for HNSCC including surgery, chemotherapy, and radiation, little progress has been made over the past 30 years in improving overall survival rates. Tumor cell death induced by both conventional and targeted chemotherapy is often mediated by the BCL-2 family-dependent mitochondrial apoptotic pathway. However, initiators of this apoptotic pathway, such as p53, are more than 50% of the time mutated or deleted in HNSCC rendering the disease refractory to treatment. To …

Hydrogen Peroxide And Pharmacological Agent Modulation Of Trpv2 Channel Gating, Tuoxin Cao

Theses and Dissertations

Transient receptor potential vanilloid 2 channel (TRPV2) is a Ca²⁺-permeable ion channel that is highly expressed in leukocytes but is also present in skeletal and cardiac muscle and endocrine cells. The TRPV2 function is implicated in a number of physiological processes, including bacterial phagocytosis, pro-inflammatory cytokine production, cardiac hypertrophy, and cancer development. TRPV2 knockout mice exhibit a high incidence of perinatal mortality, arguing that the channel plays essential roles in physiology. Despite the importance of TRPV2 for normal homeostasis, the mechanisms that control TRPV2 gating in response to pharmacological agonists, heating, membrane stretch, bioactive lipids and reactive oxygen …

Patient Outcomes And Managed Care: What Was The Impact Of The State Regulatory Backlash?, Tina C. Highfill

Theses and Dissertations

Hundreds of state regulations were passed during the “managed care backlash” of the late 1990s and early 2000s. Many of these anti-managed care regulations eased or eliminated constraints on patient utilization of health care services imposed by managed care organizations. Other regulations gave managed care providers more flexibility in the way they practiced care or helped patients appeal denials of claims. Despite the effort undertaken to pass these regulations, limited research exists on whether the regulations achieved their goal. To fill this gap, this study takes advantage of the variety of regulations enacted during the managed care backlash of the …

Tissue Factor Expression, Regulation, And Signaling In Human Airway Cells, Michael D. Davis

Theses and Dissertations

Rationale: Tissue Factor (TF) is a transmembrane glycoprotein that canonically functions as the initiator of the coagulation cascade. Increased levels of TF have been associated with inflammatory airway diseases. Since lipopolysaccharide (LPS) is known to elicit and inflammatory response in airway epithelium, we hypothesized that airway epithelial cells release TF when exposed to LPS. Since TF aids in local wound healing, we also hypothesized that inhibition of TF would decrease NHBE growth. The specific aim of this work was to evaluate the effects of LPS exposure on TF production and release from airway epithelia and determine the signaling pathways involved. …

What's Haunting Jackson Ward? Race, Space, And Environmental Violence, Rachel Spraker

Theses and Dissertations

This research is about examining the way in which racialized environmental violence contributes to exploitative social relations becoming embedded in the everyday world. I argue that the space of the everyday has been produced through cycles of social relations proceeding from and/or tied to racialized environmental violence. I continue the work of critical scholars in asserting that social and environmental violence is linked in the same ideological impulse which seeks to hide itself behind a variety of alienating processes. The slow way in which environmental violence works is particularly impactful in these processes because of its attritional lethality, contributing to …

Sol-Gel Chemistry: An Advanced Technique To Produce Macroscopic Nanostructures Of Metal And Semiconductor Colloids, Lamia Nahar

Theses and Dissertations

The fascinating physical properties that arise in materials limited to dimensions of 1-100 nm have gained noteworthy interest from the scientific community. Accordingly, there has been a lot of attention paid to the synthesis of discrete nanoparticles (NPs) and they are being investigated for a range of advanced technologies. Nonetheless, efficient use of nanomaterials in device applications require them to be assembled into solid state macro-structures while retaining their unique, nanoparticulate properties. To date, most commonly investigated assembling techniques include: covalent coupling of NPs surface groups, control evaporation of the solvent to produce ordered supercrystals or non-ordered glassy films, and …

Amixicile Inhibits Anaerobic Bacteria Within An Oral Microbiome Derived From Patients With Chronic Periodontitis, Kane Ramsey

Theses and Dissertations

Periodontitis is a chronic inflammatory disease caused by pathogenic bacteria residing in a complex biofilm within a susceptible host. Amixicile is a non-toxic, readily bioavailable novel antimicrobial that targets strict anaerobes through inhibition of the activity of Pyruvate Ferredoxin Oxidoreductase (PFOR), a major enzyme mediating oxidative decarboxylation of pyruvate. Our study aimed to evaluate the efficacy of amixicile, when compared to metronidazole, in inhibiting the growth of bacteria present in a microbiome harvested from patients with chronic periodontitis.

Plaque samples were harvested from patients with severe chronic periodontitis and cultured under anaerobic conditions. The microbiomes were grown in the presence …

A Multi-Method Exploration Of The Genetic And Environmental Risks Contributing To Tobacco Use Behaviors In Young Adulthood, Elizabeth K. Do

Theses and Dissertations

Tobacco use remains the leading preventable cause of morbidity and mortality in both the United States and worldwide. Twin and family studies have demonstrated that both genetic and environmental factors are important contributors to tobacco use behaviors. Understanding how genes, the environment, and their interactions is critical to the development of public health interventions that focus on the reduction of tobacco related morbidity and mortality. However, few studies have examined the transition from adolescent to young adulthood – the time when many individuals are experimenting with and developing patterns of tobacco use. This dissertation thesis seeks to provide a comprehensive …

The Monstrous Self: Negotiating The Boundary Of The Abject, Katya Yakubov

Theses and Dissertations

Through the lens of the horror film and the fairy tale, this thesis explores the notion of the grotesque as a boundary phenomenon—a negotiation of what is self and what is other. As such, it locates the function that the monstrous and the grotesque have in the formation of a personal and social identity. In asking why we take pleasure in the perverse, I explore how permutations of guilt, victimhood, and desire can be actively rewritten, in order to construct a stable sense of self.

The Japanese Experience In Virginia, 1900s-1950s: Jim Crow To Internment, Emma T. Ito

Theses and Dissertations

This thesis addresses how Japanese and Japanese Americans may have lived and been perceived in Virginia from 1900s through the 1950s. This work focuses on their positions in society with comparisons to the nation, particularly during the “Jim Crow” era of “colored” and “white,” and after the bombing of Pearl Harbor in 1941. It highlights various means of understanding their positions in Virginia society, with emphasis on Japanese visitors, marriages of Japanese in Virginia, and the inclusion of Japanese in higher education at Roanoke College, Randolph-Macon College, William and Mary, University of Virginia, University of Richmond, Hampden-Sydney College, and Union …

Sound Perception:Encapsulating Intangible Voice Memories In A Physical Memento, Mona Mahmoud Makhlouf

Theses and Dissertations

We live in a very busy world with a variety of sensory stimulation including the olfactory, visual, tactile, and auditory. The five senses are triggered by our surroundings and help us to form meaning about the world.ⅰ Based on where someone grows up, she or he is introduced to various sites and sounds, affecting how they interpret the world. Sounds relate meaning through the association between hearing, memory and an event. Hearing is one of the learning processes, in which individuals give, receive, and store information. We typically rely on our five senses, which contribute to the process of understanding, …

Focused Attention Vs. Open Monitoring: An Event-Related Potential Study Of Emotion Regulation By Two Distinct Forms Of Mindfulness Meditation, Tarah L. Raldiris

Theses and Dissertations

This study investigated the effects of two novel forms of 8-week mindfulness meditation training, focused attention (FA) and open monitoring (OM), relative to an established training, mindfulness-based cognitive therapy (MBCT), on early emotional reactivity to negative emotional images as assessed by electroencephalography (EEG). Data on the late-positive potential (LPP) were analyzed to address whether the three mindfulness interventions attenuated the LPP from pre- to post-intervention, and if significant differences existed between groups in LPP at post-intervention. Rather than an attenuation, results indicated an average increase in LPP amplitude from pre- to post-intervention. No significant differences were found in the LPP …

Should We Straighten Up? Exploring The Responsibilities Of Actor Training For Lgbtq Students, Matthew B. Ferrell

Theses and Dissertations

Gay actors have a long history with the notion of “straightening up” to remain castable and economically feasible in today’s market. Searching to find answers for young acting students while strengthening their own self worth, I will explore the history of gay actors in film, television and theatre and in society to understand this notion more fully. By interviewing working actors and managers in the business I will explore how I can address this question of “straightening up” to the future generation of actors and analyze how we can face the future with integrity and self-respect.

Women Self Actualization: A Narrative Of A Performative Gender Constitution, Hala A. Gabr

Theses and Dissertations

In a traditional Middle Eastern society, men and women have been confined within society’s gender definitions. Those imposed social constructs condition men differently from women by dictating expected behaviors, establishing a hierarchy of gender positioning and enforcing definitions that limit abilities and potential.

Based on postmodernist philosopher, feminist and social theorist, Simone de Beauvoir and postmodernist American philosopher and gender theorist Judith Butler, gender is not an inborn role, but rather created through stylized repetition of acts informed by society, named performative acts. For de Beauvoir and Butler, gender can never be a stable identity (Butler 1988).

Informed by Butler’s …