Digital Commons Network^™

Lifetime, Emily E. Kuchenbecker

Theses and Dissertations

Time is my bully. Time marks the start of something, as well as the end. We are all carrying out the inexorable passing of time as it relates to our impending mortalities.

I do not fear death.

The awareness of my body’s impermanence employs me to feel that much more connected to the vessel containing that of which I am.

But what am I? Am I my body- or is it much deeper?

Through the work executed during my graduate research, I have attempted to quantify my existence through the archiving my time and body. This document ushers you through …

Graphic Content Warning; Personal And Political Traumas, Emily K. Wardell

Theses and Dissertations

The written portion of this thesis work is meant to address and further investigate the visual work created using mediums of print and found video. This artistic research has been interested in examining varying associations with truth, recollection, and evidence. This includes the recollection of public histories and news-media narratives as well as my own history and trauma. Through this work my aim was to create a deconstruction and revolt against how associations are formed, and how to understand imagery as information. This thesis first discusses my relationship to appropriated imagery, then connects and examines it through the addition of …

Aeg-1 Knockout Sensitizes Hepatocellular Carcinoma (Hcc) Cells To Ionizing Radiation, Maheen Khan

Theses and Dissertations

Liver cancer is the fourth leading cause of cancer-associated deaths globally, and among primary liver cancers, hepatocellular carcinoma (HCC) encompasses 75-85% of all cases. HCC is a highly lethal disease due to limited treatment options – only a small subset of patients qualify for surgical resection or transplantation; the remaining patients often display resistance to radiation therapy or chemotherapy. Overexpression of the oncogene astrocyte elevated gene-1 (AEG-1) is associated with poorer survival and increased tumor recurrence in HCC, and numerous studies show its role in initiation of hepatocarcinogenesis. A prior study also demonstrated AEG-1 expression inhibits senescence by diminishing the …

The Mountains At The End Of The World: Subcultural Appropriations Of Appalachia And The Hillbilly Image, 1990-2010, Paul L. Robertson

Theses and Dissertations

There is an aversion within the field of Appalachian Studies to addressing the cultural formulations of the Appalachian/hillbilly/mountaineer as an icon of aggressive resistance. The aversion is understandable, as for far too long images of the irrationally and savagely violent mountaineer were integral to the most gross popular culture stereotypes of Appalachia. Media consumers often take pleasure or comfort in these images, which usually occur in a reactionary context with the hillbilly as either a type of nationally necessary savage OR as an unregenerate barbarian against whom a national civilization will triumph and benefit by the struggle.

I bookend my …

Regenerative Potential Of Mesenchymal Stem Cell Derived Exosomes, Andrew P. Wong

Theses and Dissertations

Bone defects are a pervasive complication arising from many clinical conditions, both mechanical and pathological. Current treatments for large bony defects focus on applying bone grafts or synthetic materials to the defect area. Cell-based—and especially stem-cell—therapies have advanced greatly thanks to increasing attention focused on their ability to generate new tissues in situ with biomechanical properties approaching that of native tissue, but they suffer from their own shortcomings as well. Exosomes have been shown to play critical roles in cell-signaling and tissue regeneration and are therefore potentially ideal therapeutic vehicles for treating bone defects. Exosomes are small microvesicles counted amongst …

Fluvastatin And Microrna-146a Alter Interleukin-33 Mediated Mast Cell Functions., Marcela Taruselli

Theses and Dissertations

Mast cells are tissue-resident immune cells known as effector cells for the innate and adaptive immune systems. Mast cells contribute to host defenses against parasites such as large roundworm parasites, bacterial pathogens, and toxins, and participate in wound healing, but they are mostly known for their role in allergic diseases. It has been well established that during allergic diseases, mast cells are stimulated by IgE cross-linkage to release proinflammatory mediators. However, a newly discovered cytokine, IL-33 has also been implicated in allergic disease. Recently, IL-33 has been implicated as a driver of several Type I sensitivities and previous studies have …

Light In Community: A Study In The Adaptive Reuse Of Sacred Space, Abigail Barras

Theses and Dissertations

ABSTRACT

MOTIVATION
American life is increasingly fragmented, leading to a sense of restlessness and disconnection. Much of that fragmentation can be traced to our pattern of architectural and sociological development, namely, the rise of the automobile suburbs in the 1950s and 60s and the abandonment of densely populated, human- scaled environments like that of the small town or city center (Oldenberg, 1999).

PROBLEM
Large numbers of architecturally significant buildings have fallen into disrepair over the years following the “white flight” of the 1960s and 70s, during which significant segments of investment dollars left city centers and followed to the suburbs …

The Role Of Kras In Mechanosensing In Non-Small Cell Lung Cancer, Krista M. Powell

Theses and Dissertations

Lung cancer is the number one cause of cancer related death worldwide, with more than 1.6 million fatalities each year. Non-small cell lung cancer (NSCLC) accounts for 80-85% of all lung cancers, with KRAS being one of the most prevalent oncogenic driver mutations. Therapeutic approaches for KRAS-mutated NSCLC have been extensively explored due to the US National Cancer Institute RAS Initiative, but methods of directly targeting KRAS or downstream effectors, such as MEK, still have poor results. Previous reports have shown that KRAS-mutated NSCLC activate distinct receptor tyrosine kinases (RTKs) depending on the epithelial or mesenchymal state. Epithelial-to-mesenchymal transition (EMT) …

Silver Sands, David Riley

Theses and Dissertations

Silver Sands is a thirty-minute documentary film and accompanying installation that traces the story of Marc Hampton, a gay, desert-dwelling Vietnam vet, former Playgirl model, and vintage car collector who lost two lovers to AIDS. Drawing on videotaped interviews, shots of his house and environs, and extensive use of his personal photographic archive, this work addresses evolving ideas of memory and representation within the queer community. The film is divided into various chapters which function as meditations on masculinity, aging, loss, spirituality and intergenerational relationships. Marc becomes an archetypal figure––the survivor––whose meandering recollections illustrate some of the complexities of gay …

Weaponization Of Space: Subverting The Architecture Of Occupation, Majdulin Nasrallah

Theses and Dissertations

Israel’s illegal occupation of Palestine and oppression of the Palestinian people has manifested itself in countless ways. The built environment, fueled by spatial theory, has been transformed into an instrument of war that serves a military agenda. In this context, the physical environment is not simply the arena of conflict, but a weapon wielded by occupying forces. This research investigates spatial control through seemingly mundane architecture and military practices, which are deployed deliberately to strangulate Palestinian livelihood and prosperity. Derived from Deleuze and Guittari’s delineation of smooth and striated space, with an emphasis on walls and barriers, this thesis subverts …

Spag17 Deficiency Impairs Neuronal Cell Differentiation In Developing Brain, Olivia J. Choi

Theses and Dissertations

The development of the nervous system is a multi-level, time-sensitive process that relies heavily on cell differentiation. However, the molecular mechanisms that control brain development remain poorly understood. We generated a knockout (KO) mouse for the cilia associated gene Spag17. These animals develop hydrocephalus and enlarged ventricles consistent with the role of Spag17 in the motility of ependymal cilia. However, other phenotypes that cannot be explained by this role were also present. Recently, a mutation in Spag17 has been associated with brain malformations and severe intellectual disability in humans. Therefore, we hypothesized that Spag17 plays a crucial role in …

The Wheel House - An Intergenerational Space Creating Bonds Between At-Risk Teens And Seniors, Jessica M. Keegan

Theses and Dissertations

MOTIVATION

Two main groups in American society today are floundering: adolescent children of low income families and the elderly.

Between the hours of 3:00 and 6:00 p.m., one in five children are unsupervised. Left to their own devices, many become involved in negative behaviors such as drug and alcohol abuse, sexual activity or in the worse cases, delinquency (Afterschool Alliance, 2016). It is proven that when children are alone after school, they not only miss out on valuable learning opportunities, but also their parents are affected as well by having to lose as many as eight work days annually to …

Erasure: An Additive And Subtractive Act, Margaret Davids

Theses and Dissertations

MOTIVATION In the simplest form, a pencil mark on a page is removed by a traditional rubber eraser. However, the marks are often never fully removed, and the paper thins with each attempt to rub out an old idea.

But how does one erase a chair? A pilaster? A room? A building?... More importantly, how does the subtractive act of erasing become an additive one?

The historical fabric of a building is important; it is also imperative that it does not remain stagnant. Erasing is an opportunity to design an interior environment that both acknowledges the traces of the pencil …

Pharmacodynamics Of Monoamine Transporter Releasing Agents And Reuptake Inhibitors, Alexa Holloway

Theses and Dissertations

Ligands of the human monoamine transporters encompass a wide range of both illicit and therapeutic drugs that act upon neural circuitry related to reward, motivation, and the processing of salient stimuli. The present study utilizes two methods for analyzing transporter substrates and inhibitors in order to characterize activity and assess potency. The first measures transient changes in intracellular calcium as a surrogate for transporter activity by harnessing the electrical coupling of monoamine transporters and L-type calcium channels. This is used to analyze novel chimera of the strong hDAT inhibitors methylphenidate and ��-PPP in order to assess the contribution of specific …

Out Of The Margins: Evolving Narrative Representation Of Women In Video Games, Rowan Lucas

Theses and Dissertations

This thesis examines narrative representation of female characters in video games and how game narratives and representations contribute to socio-cultural discourse. First, this thesis explores and defines the cultural background for female representation in video games. It then defines video games as a type of text and describes the features that are unique to games, such as the use of avatars, and what impacts these features have on game narratives. The thesis attempts to establish evidence of an evolutionary arc of comprehensive female representation in video games by first exploring historical female narrative tropes, and then comparing them to narrative …

Generic Of, Nicole Levaque

Theses and Dissertations

generic of is a creative text paralleling the creation of my thesis exhibition. I use fragmented layers of narrative, description and prose in the same way each handbuilt ceramic is fired multiple times, allowing the glazes to build upon themselves. This is a close study of the still life, the intimacy of consuming, and how trauma is passed through the gut.

Whirling Hybrids: A Dichotomy Of Belonging, Rabeya Khatoon

Theses and Dissertations

Migration is a phenomenon wherein individuals relocate from one country to another, albeit temporarily or permanently, for numerous reasons. The State of Qatar is a highly diverse nation with a large population of foreign residents. According to Priya D’Souza, as of 2017, 60 percent of the resident population in Qatar are from South Asia. Growing up in this environment, third culture kids develop a unique, hybrid culture through experiencing multiple cultures. This research investigates a dichotomy of belonging from the perspective of South Asians in Qatar. A series of hybridized spinning tops were produced in collaboration with a South Asian …

Humans Aren't Boxes, Art Isn't Finite, Brianne Alta Humphreys

Theses and Dissertations

I am bored. All around me are systems that perpetuate repetitive, reductive, and mundane modes of living. In an attempt to counter a culture obsessed with singular ways of existence and bite-sized perfection, I utilize moving mediums of video and performance to dive head first into a vast array of sloppy sincerity. The crisp, white-washed, analytical, and restrictive is loudly replaced with the empirical, haphazard, and instinctual. My intention is to create and encourage raw, performative-based work that is as multifaceted as unbridled life itself. This alive and physical practice hosts a conglomeration of sweat, memories, heartbreaks, hymn singing, line …

Manufactured By Nature: Growing Generatively Designed Products, Mohammad Jawad

Theses and Dissertations

Mass production and assembly lines are yesterday’s manufacturing methods. They have exhausted Earth’s resources and limited the possibilities of design in terms of both form and material, prompting designers to search for new processes. A new generation of making includes biomimicry-inspired technologies such as 3D printing and parametric simulation, which have transformed the production paradigm. Utilizing nature as industry, this thesis explores the possibility of “growing” designed objects by employing nature’s own processes and resources. It integrates bio materials, generative design and additive manufacturing to produce objects for a post-industrial world. The project outcomes employ natural minerals, crystallization and 3D …

Lotion In Your Lungs, Raul H. De Lara

Theses and Dissertations

This is a document explaining in detail my artistic practice from childhood to the day I graduated VCU. It will perhaps only be understood by those who have themselves already felt such ways, or similar ways – words and ghosts are mostly invisible.

Graphic Intimacies: Identity, Humor, And Trauma In Autobiographical Comics By Women Of Color, Francesca Lyn

Theses and Dissertations

Graphic Intimacies: Identity, Humor, and Trauma in Autobiographical Comics by Women of Color examines works of comics art about the lived experience of the comics’ creator. These graphic narratives address racialized difference and the construction of identity while also using humor to negotiate their narrations of traumatic events. I argue that these creators employ the structure of comics to replicate the fragmentary nature of memory. Comics allow for the representation of trauma as being intimately linked to corporeality. The comics medium allows creators to make visible and present fractured versions of the self, a product of traumatic fragmentation. Drawing traumatic …

An Evaluation Of Induced Shear Stress On Endothelial Cellular Adhesion Molecules, Edward B. Crabb

Theses and Dissertations

The pathophysiology of atherosclerotic cardiovascular disease (CVD) is highlighted by vascular dysfunction and low-grade vascular inflammation. Furthermore, the site-specific distribution of atherosclerosis throughout the arterial vasculature is primarily determined by local hemodynamic force. Therefore, this dissertation outlines three experiments designed to investigate the role of acute mental and physical (i.e., aerobic exercise), and vascular wall shear stress (SS) on the inflammatory aspects of atherosclerosis. Chapter 2 examines the effect of acute laboratory-induced mental stress on intracellular pro-inflammatory signaling pathways in peripheral blood mononuclear cells. Chapter 3 investigates the impact of acute laboratory-induced mental stress and maximal aerobic exercise on the …

Computational And Experimental Investigation On The Wetting Behavior Of Droplet-Fiber Systems, Hossain Aziz

Theses and Dissertations

Interaction of a liquid droplet and a fiber or layer of fibers is ubiquitous in nature and in a variety of industrial applications. It plays a crucial role in fog harvesting, coalescence filtration, membrane desalination, self-cleaning and fiber based microfluidics, among many others. This work presents a quantitative investigation on the interactions of a droplet with a fiber or layers of fibers. More precisely, the present work is focused on 1) predicting the effects of fiber’s size and material on its ability to withhold a droplet against external forces and on the liquid residue left on the fiber after the …

Singing The Landscape: A Meditation On Song, Sound And Community At The Fall Line Of The James River, Sara Bouchard

Theses and Dissertations

I work in the medium of song. A multidisciplinary artist and composer, I make work that is immersive, time-based and often participatory. I interact with landscape and the complexities of American history, bringing into focus local ecologies through the lens of song.

This document accompanies my thesis performance The Sound of a Stone, an immersive exploration of song, language, ecology and locational listening performed in a 4-channel surround format. In the semi-improvised composition, I sample live vocals, mandolin and found natural objects in a combination of roots music traditions and experimental techniques. Utilizing the software Ableton Live to process …

The Wild Beasts, Peter Cochrane

Theses and Dissertations

The Wild Beasts springs from my desire to thank my ever-expanding queer chosen family and mentors for their strength. Working through the often violent and othering aspects of the lens and photographic histories I create floral portraits responding to each person’s being and our relationship. Using the 19th century, 8x10 large format view camera—the same used by colonialists and ethnographers to “capture” the divinity of Nature—I erect each as a traditional still life studio setup at the threshold between the natural world and that constructed by humans. These environments speak both to the character of each friend and also to …

The Puppets Look Like Flowers At Last, Evie Metz

Theses and Dissertations

The urge to uncover aspects of human condition permeates my work, from the fundamental curiosity of a child tearing apart their doll to uncover what lies within to continuing a quest in uncovering basic human urges through my puppet animated dramas and tragedies. There is a controversial line between the childlike and the adult-like that can be ambiguous, and at some times more discernible while other times less. I create handcrafted stop-frame puppet animations that explore self-conscious emotions such as embarrassment, shame, and envy within unpredictable life scenarios. These are animations about inner life, attempting to resolve conflicting elements of …

Nigga Is Historical: This Is Not An Invitation For White People To Say Nigga, Sandy Williams Iv

Theses and Dissertations

Over the past several years I have been on a quest to locate a world beyond the one I’ve been presented. I am interested in the history of atomic particles - like everything that radiates off of a monument (both literally and those things that are metaphorically reified) - invisible things, and the ways in which these things insect beyond our knowledge systems. This inquiry takes many forms. Mine is a conceptually based practice linked to record keeping and time, and the ways in which these concepts find plurality within our culture; or more pointedly, the importance that we attach …

Multiphase Droplet Interactions With A Single Fiber, Noor M. Farhan

Theses and Dissertations

Abstract

Multiphase Droplet Interactions with a Single Fiber

By: Noor M. Farhan

A dissertation submitted in partial fulfillment of the requirements for the degree of Doctor of Philosophy at Virginia Commonwealth University.

Virginia Commonwealth University, 2019

Director: Hooman V. Tafreshi,

Professor, Department of Mechanical and Nuclear Engineering

Formulating the physics of droplet adhesion to a fiber is interesting intellectually and important industrially. A typical example of a droplet–fiber system in nature is the dew droplets on spider webs, where droplets first precipitate and grow on the fibers, but they eventually fall when they become too heavy. Obviously, quantifying the force …

Parallel Narrative: Short-Video Social Media Platforms’ Influences On Contemporary Narrative, Ruiqi Zhang

Theses and Dissertations

Through the study of Kwai, a popular short-video social media platform in China, this thesis investigates the social issues, media class divides and aesthetics specific to Kwai culture. It further proposes a strategy of artistic practice - parallel narrative - an experiment in video art production and editing techniques that explores new possibilities of narrative in video art. Integrating theoretical research on Post-Internet art and object-oriented ontology, this thesis reveals people’s ability to digest multisource information and shows how mobile technologies and open-source materials contribute to the formation of parallel narratives.

Tone And Texture, Leather And Lace : A Case For Making Strong Choices In The Costume Design For The Three Musketeers., Emily L. Tappan

Theses and Dissertations

This thesis explores and describes key factors in the design process leading to production of The Three Musketeers by Catherine Bush. The document encompasses the justification and discussion of the choices made during the research, design, and production stages of developing the play for the stage, as well as impressions gained throughout the process to use in future design projects.