Digital Commons Network^™

Minocycline Modulates Antigen-Specific Ctl Activity Through Inactivation Of Mononuclear Phagocytes In Patients With Htlv-I Associated Neurologic Disease, Yoshimi Enose-Akahata, Eiji Matsuura, Yuetsu Tanaka, Unsong Oh, Steven Jacobson

Neurology Publications

Background The activation of mononuclear phagocytes (MPs), including monocytes, macrophages and dendritic cells, contributes to central nervous system inflammation in various neurological diseases. In HTLV-I-associated myelopathy/tropical spastic paraparesis (HAM/TSP), MPs are reservoirs of HTLV-I, and induce proinflammatory cytokines and excess T cell responses. The virus-infected or activated MPs may play a role in immuneregulation and disease progression in patients with HTLV-I-associated neurological diseases.

Results Phenotypic analysis of CD14+ monocytes in HAM/TSP patients demonstrated high expression of CX3CR1 and HLA-DR in CD14lowCD16+ monocytes, compared to healthy normal donors (NDs) and asymptomatic carriers (ACs), and the production of TNF-α and IL-1β in …

Rna-Seq Analysis Implicates Dysregulation Of The Immune System In Schizophrenia, Junzhe Xu, Jingchun Sun, Jingchun Chen, Lily Wang, Anna Li, Matthew Helm, Steven L. Dubovsky, Silviu-Alin Bacanu, Zhongming Zhao, Xiangning Chen

Psychiatry Publications

Background While genome-wide association studies identified some promising candidates for schizophrenia, the majority of risk genes remained unknown. We were interested in testing whether integration gene expression and other functional information could facilitate the identification of susceptibility genes and related biological pathways.

Results We conducted high throughput sequencing analyses to evaluate mRNA expression in blood samples isolated from 3 schizophrenia patients and 3 healthy controls. We also conducted pooled sequencing of 10 schizophrenic patients and matched controls. Differentially expressed genes were identified by t-test. In the individually sequenced dataset, we identified 198 genes differentially expressed between cases and controls, of …

Identification And Structural Characterization Of A New Three-Finger Toxin Hemachatoxin From Hemachatus Haemachatus Venom, Vallerinteavide Mavelli Girish, Sundramurthy Kumar, Lissa Joseph, Chacko Jobichen, R. Manjunatha Kini, J. Sivaraman

Biochemistry and Molecular Biology Publications

Snake venoms are rich sources of biologically active proteins and polypeptides. Three-finger toxins are non-enzymatic proteins present in elapid (cobras, kraits, mambas and sea snakes) and colubrid venoms. These proteins contain four conserved disulfide bonds in the core to maintain the three-finger folds. Although all three-finger toxins have similar fold, their biological activities are different. A new three-finger toxin (hemachatoxin) was isolated from Hemachatus haemachatus (Ringhals cobra) venom. Its amino acid sequence was elucidated, and crystal structure was determined at 2.43 Å resolution. The overall fold is similar to other three-finger toxins. The structure and sequence analysis revealed that the …

Metabolic Rate Regulates L1 Longevity In C. Elegans, Inhwan Lee, Amber Hendrix, Jeongho Kim, Jennifer Yoshimoto, Young-Jai You

Biochemistry and Molecular Biology Publications

Animals have to cope with starvation. The molecular mechanisms by which animals survive long-term starvation, however, are not clearly understood. When they hatch without food, C. elegans arrests development at the first larval stage (L1) and survives more than two weeks. Here we show that the survival span of arrested L1s, which we call L1 longevity, is a starvation response regulated by metabolic rate during starvation. A high rate of metabolism shortens the L1 survival span, whereas a low rate of metabolism lengthens it. The longer worms are starved, the slower they grow once they are fed, suggesting that L1 …

Early Hearing-Impairment Results In Crossmodal Reorganization Of Ferret Core Auditory Cortex, M. Alex Meredith, Brian L. Allman

Anatomy and Neurobiology Publications

Numerous investigations of cortical crossmodal plasticity, most often in congenital or early-deaf subjects, have indicated that secondary auditory cortical areas reorganize to exhibit visual responsiveness while the core auditory regions are largely spared. However, a recent study of adult-deafened ferrets demonstrated that core auditory cortex was reorganized by the somatosensory modality. Because adult animals have matured beyond their critical period of sensory development and plasticity, it was not known if adult-deafening and early-deafening would generate the same crossmodal results. The present study used young, ototoxically-lesioned ferrets () that, after maturation (avg. = 173 days old), showed significant hearing deficits (avg. …

Optogenetic Stimulation Of The Corticothalamic Pathway Affects Relay Cells And Gabaergic Neurons Differently In The Mouse Visual Thalamus, Chris W. D. Jurgens, Karen A. Bell, A. Rory Mcquiston, William Guido

Anatomy and Neurobiology Publications

The dorsal lateral geniculate nucleus (dLGN) serves as the primary conduit of retinal information to visual cortex. In addition to retinal input, dLGN receives a large feedback projection from layer VI of visual cortex. Such input modulates thalamic signal transmission in different ways that range from gain control to synchronizing network activity in a stimulus-specific manner. However, the mechanisms underlying such modulation have been difficult to study, in part because of the complex circuitry and diverse cell types this pathway innervates. To address this and overcome some of the technical limitations inherent in studying the corticothalamic (CT) pathway, we adopted …

Virginia Commonwealth University Undergraduate Bulletin [2012], Virginia Commonwealth University

VCU Bulletins

Undergraduate bulletin for Virginia Commonwealth University for the academic year 2012-2013. It includes information on academic regulations, degree requirements, course offerings, faculty, academic calendar, and tuition and expenses for undergraduate programs.

Virginia Commonwealth University Undergraduate Bulletin Courses [2012], Virginia Commonwealth University

VCU Bulletins

Listing of undergraduate courses for 2012-2013.

An Observational Study On Cough In Children: Epidemiology, Impact On Quality Of Sleep And Treatment Outcome, Francesco De Blasio, Peter V. Dicpinigaitus, Bruce K. Rubin, Gianluca De Danieli, Luigi Lanata, Alessando Zanasi

Pediatrics Publications

Background Cough is one of the most frequent symptoms in children and is the most common symptom for which children visit a health care provider.

Methods This is an observational study on acute cough associated with upper respiratory tract infection (URTI) in children. The study evaluates the epidemiology and impact of cough on quality of sleep and children's activities, and the outcome of cough with antitussive treatments in pediatric routine clinical practice. Study assessments were performed through a pediatric cough questionnaire (PCQ), developed by the Italian Society of Cough Study. A total of 433 children visited by family care pediatricians …

Infantile Spasms (West Syndrome): Update And Resources For Pediatricians And Providers To Share With Parents, James W. Wheless, Patricia A. Gibson, Kari Luther Rosbeck, Maria Hardin, Christine O’Dell, Vicky Whittemore, John M. Pellock

Neurology Publications

Background Infantile spasms (IS; West syndrome) is a severe form of encephalopathy that typically affects infants younger than 2 years old. Pediatricians, pediatric neurologists, and other pediatric health care providers are all potentially key early contacts for families who have an infant with IS. The objective of this article is to assist pediatric health care providers in the detection of the disease and in the counseling and guidance of families who have an infant with IS.

Methods Treatment guidelines, consensus reports, and original research studies are reviewed to provide an update regarding the diagnosis and treatment of infants with IS. …

Transfer Student Peer Mentorship Initiative, Grace E. Harris Leadership Institute, Virginia Commonwealth University, Melinda Debrew, Artis Gordon, Rebecca Jones, Teri Kuttenkuler, Irene Lubker, Dewey Taylor

L. Douglas Wilder School of Government and Public Affairs Publications

Transfer students have unique needs that differ from those of incoming freshmen. The TRAM Transfer Student Mentorship Program connects incoming transfer students with students who also began their VCU journey as transfer students. Through a one-on-one relationship with a trained mentor, transfer students who participate in this program will access guidance and information to help them navigate the university and its resources, programs, and culture.

The Administrative And Professional Faculty Experience At Vcu, Grace E. Harris Leadership Institute, Virginia Commonwealth University, Leslie Bozeman, Melanie Christian, Colleen Davis, Harold Harris Jr., Sydney Mcdonald, Nicolle Parsons-Pollard

L. Douglas Wilder School of Government and Public Affairs Publications

At a time when Virginia Commonwealth University (VCU) is striving to become a premier research institution, Administrative and Professional (A&P) Faculty are essential to the university’s ability to provide and sustain the quality educational programs that are the foundation of the academic enterprise. While essential, A&P Faculty are not always factored into University efforts to the same extent as instructional faculty. Recognizing that A&P Faculty represent a population diverse in educational background, professional focus, role, responsibility, and contribution, Team Our Time conducted a mixed methods study on the experience of A&P Faculty at VCU.

A Racial Impact Analysis Of Hb 1075/Sb 201, Grace E. Harris Leadership Institute, Virginia Commonwealth University, Patrice Banks, Cydny Neville, Sookyung Oh, Keith Rogers Jr.

L. Douglas Wilder School of Government and Public Affairs Publications

The economic and social consequences of untreated (or under-treated) substance abuse among minors are significant. This report provides a racial impact analysis of HB 1075/SB 201, legislation approved in the 2012 General Assembly session that seeks to improve access and use of substance and alcohol services by minors. In short, this policy could go a long way to ensure that families are properly educated about these life-changing (and life-saving) programs; however our analysis raises concerns related to cultural competency that may serve to undermine the legislation’s goal. Virginia is incredibly diverse and its communities vary widely with its assets and …

A Racial Impact Analysis Of Hb 462, Grace E. Harris Leadership Institute, Virginia Commonwealth University, Daphne Bryan, William Randolph, Tarvaris Mccoy

L. Douglas Wilder School of Government and Public Affairs Publications

The adoption of House Bill 462 into law brings forth questions about the probable impact on the physical, mental, emotional and financial well-being of the women in Virginia. In particular, analysis of its effect on the minority and underserved female population is required. Understanding the origin of this new legislation and the thinking behind its proposal and subsequent adoption dictates an endeavor into the backgrounds and intended goals of its sponsors and supporters. Proponents of HB 462 were unresponsive to requests for an interview to expound on their perspective about the importance of the legislation. This precipitated the use of …

Virginia Dental Journal (Vol. 89, No. 3, 2012)

Virginia Dental Journal

No abstract provided.

A Racial Impact Analysis Of Hb 869/Sb 274, Grace E. Harris Leadership Institute, Virginia Commonwealth University, Ashley Chapman, Ida Jones, Donald Knight, Susan Laywer Mills

L. Douglas Wilder School of Government and Public Affairs Publications

Virginia is growing. Between 1990 and 2000, Virginia’s population rose by over 14% - the largest population growth experienced by the state in a single decade (Pollard, 2007). And the state continues to grow. It has been estimated that by 2030, Virginia’s total population may exceed 9.8 million people (Pollard, 2007). Along with population growth, land development has increased which in turn affects transportation, housing and job placement across the Commonwealth. In fact, in many parts of the state, development has outpaced population growth. According to Pollard, “If current patterns continue, Virginia will develop more land in the next 40 …

A Racial Impact Analysis Of Hb 9, Grace E. Harris Leadership Institute, Virginia Commonwealth University, Olutosin Burrell, Dawn Lawson, Robert Mayfield, Ladonna Sanders

L. Douglas Wilder School of Government and Public Affairs Publications

House Bill 9 was introduced by Delegate Mark L. Cole (R – House District 88 – Fredericksburg) as chief patron with support from co-patron Delegate Charles D. Poindexter (R – House District 9 – Glade Hill). The bill states that a voter who is unable to present one of the forms of ID may sign a sworn statement that he/she is the named registered voter he/she claims to be and then be allowed to vote a provisional ballot. The current law allows such a voter to cast an official ballot rather than a provisional one after signing such statement. We …

Crystal Structures Of Human Pyridoxal Kinase In Complex With The Neurotoxins, Ginkgotoxin And Theophylline: Insights Into Pyridoxal Kinase Inhibition, Amit Gandhi, Jigar V. Desai, Mohini S. Ghatge, Martino L. Di Salvo, Stefano Di Biase, Richmond Danso-Danquah, Faik N. Musayev, Roberto Contestabile, Verne Schirch, Martin A. Safo

Medicinal Chemistry Publications

Several drugs and natural compounds are known to be highly neurotoxic, triggering epileptic convulsions or seizures, and causing headaches, agitations, as well as other neuronal symptoms. The neurotoxic effects of some of these compounds, including theophylline and ginkgotoxin, have been traced to their inhibitory activity against human pyridoxal kinase (hPL kinase), resulting in deficiency of the active cofactor form of vitamin B6, pyridoxal 5′-phosphate (PLP). Pyridoxal (PL), an inactive form of vitamin B6 is converted to PLP by PL kinase. PLP is the B6 vitamer required as a cofactor for over 160 enzymatic activities essential in primary and secondary metabolism. …

Species-Level Classification Of The Vaginal Microbiome, Jennifer M. Fettweis, Myrna G. Serrano, Nihar U. Sheth, Abigail L. Glascock, J. Paul Brooks, Kimberly K. Jefferson, Vaginal Microbiome Consortium, Gregory A. Buck

Microbiology and Immunology Publications

Background

The application of next-generation sequencing to the study of the vaginal microbiome is revealing the spectrum of microbial communities that inhabit the human vagina. High-resolution identification of bacterial taxa, minimally to the species level, is necessary to fully understand the association of the vaginal microbiome with bacterial vaginosis, sexually transmitted infections, pregnancy complications, menopause, and other physiological and infectious conditions. However, most current taxonomic assignment strategies based on metagenomic 16S rDNA sequence analysis provide at best a genus-level resolution. While surveys of 16S rRNA gene sequences are common in microbiome studies, few well-curated, body-site-specific reference databases of 16S rRNA …

Peripheral-Blood Stem Cells Versus Bone Marrow From Unrelated Donors, Claudio Anasetti, Brent R. Logan, Stephanie J. Lee, Edmund K. Waller, Daniel J. Weisdorf, John R. Wingard, Corey S. Cutler, Peter Westervelt, Ann Woolfrey, Stephen Couban, Gerhard Ehninger, Laura Johnston, Richard T. Maziarz, Michael A. Pulsipher, David L. Porter, Shin Mineishi, John M. Mccarty, Shakila P. Khan, Paolo Anderlini, William I. Bensinger, Susan F. Leitman, Scott D. Rowley, Christopher Bredeson, Shelly L. Carter, Mary M. Horowitz, Dennis L. Confer

Massey Comprehensive Cancer Center Publications

BACKGROUND

Randomized trials have shown that the transplantation of filgrastim-mobilized peripheral-blood stem cells from HLA-identical siblings accelerates engraftment but increases the risks of acute and chronic graft-versus-host disease (GVHD), as compared with the transplantation of bone marrow. Some studies have also shown that peripheral-blood stem cells are associated with a decreased rate of relapse and improved survival among recipients with high-risk leukemia.

METHODS

We conducted a phase 3, multicenter, randomized trial of transplantation of peripheral-blood stem cells versus bone marrow from unrelated donors to compare 2-year survival probabilities with the use of an intention-to-treat analysis. Between March 2004 and September …

Alcohol Availability And Neighborhood Poverty And Their Relationship To Binge Drinking And Related Problems Among Drinkers In Committed Relationships, Christy M. Mckinney, Karen G. Chartier, Raul Caetano, T. Robert Harris

Social Work Publications

The authors examined the relationship of alcohol outlet density (AOD) and neighborhood poverty with binge drinking and alcohol-related problems among drinkers in married and cohabitating relationships and assessed whether these associations differed across sex. A U.S. national population couples survey was linked to U.S. Census data on AOD and neighborhood poverty. The 1,784 current drinkers in the survey reported on their binge drinking, alcohol-related problems, and other covariates. AOD was defined as the number of alcohol outlets per 10,000 persons and was obtained at the zip code level. Neighborhood poverty was as having a low (<20%) or high (≥20%) proportion of residents living in poverty at the census tract level. We used logistic regression for survey data to estimate odds ratios and 95% confidence intervals and tested for differences of associations by sex. Associations of neighborhood poverty with binge drinking were stronger for male than for female drinkers. The association of neighborhood poverty with alcohol-related problems was also stronger for men than for women. We observed no relationships between AOD and binge drinking or alcohol-related problems in this couples survey. Efforts to reduce binge drinking or alcohol-related problems among partners in committed relationships may have the greatest impact if targeted to male drinkers living in high-poverty neighborhoods. Binge drinking and alcohol-related problems, as well as residence in an impoverished neighborhood are risk factors for intimate partner violence (IPV) and other relationship conflicts.

Discovery Of Prostamide F2Α And Its Role In Inflammatory Pain And Dorsal Horn Nociceptive Neuron Hyperexcitability, Luisa Gatta, Fabiana Piscitelli, Catia Giordano, Serana Boccella, Aron H. Lichtman, Sebatino Maione, Vincenzo Di Marzo

Pharmacology and Toxicology Publications

It was suggested that endocannabinoids are metabolized by cyclooxygenase (COX)-2 in the spinal cord of rats with kaolin/λ-carrageenan-induced knee inflammation, and that this mechanism contributes to the analgesic effects of COX-2 inhibitors in this experimental model. We report the development of a specific method for the identification of endocannabinoid COX-2 metabolites, its application to measure the levels of these compounds in tissues, and the finding of prostamide F2α (PMF2α) in mice with knee inflammation. Whereas the levels of spinal endocannabinoids were not significantly altered by kaolin/λ-carrageenan-induced knee inflammation, those of the COX-2 metabolite of AEA, PMF2α, were strongly elevated. The …

Comparison Of Walking Overground And In A Computer Assisted Rehabilitation Environment (Caren) In Individuals With And Without Transtibial Amputation, Deanna H. Gates, Benjamin J. Darter, Jonathan B. Dingwell, Jason M. Wilken

Physical Therapy Publications

Background

Due to increased interest in treadmill gait training, recent research has focused on the similarities and differences between treadmill and overground walking. Most of these studies have tested healthy, young subjects rather than impaired populations that might benefit from such training. These studies also do not include optic flow, which may change how the individuals integrate sensory information when walking on a treadmill. This study compared overground walking to treadmill walking in a computer assisted virtual reality environment (CAREN) in individuals with and without transtibial amputations (TTA).

Methods

Seven individuals with traumatic TTA and 27 unimpaired controls participated. Subjects …

The Effects Of Diffusion On An Exonuclease/Nanopore-Based Dna Sequencing Engine, Joseph E. Reiner, Arvind Balijepalli, Joseph W. F. Robertson, Bryon S. Drown, Daniel L. Burden, John J. Kasianowicz

Physics Publications

Over 15 years ago, the ability to electrically detect and characterize individual polynucleotides as they are driven through a single protein ion channel was suggested as a potential method for rapidly sequencing DNA, base-by-base, in a ticker tape-like fashion. More recently, a variation of this method was proposed in which a nanopore would instead detect single nucleotides cleaved sequentially by an exonuclease enzyme in close proximity to one pore entrance. We analyze the exonuclease/nanopore-based DNA sequencing engine using analytical theory and computer simulations that describe nucleotide transport. The available data and analytical results suggest that the proposed method will be …

The Interleukin 3 Gene (Il3) Contributes To Human Brain Volume Variation By Regulating Proliferation And Survival Of Neural Progenitors, Xiao-Jian Luo, Ming Li, Liang Huang, Khwangsik Nho, Min Deng, Qiang Chen, Daniel R. Weinberger, Alejandro A. Vasquez, Mark Rijpkema, Venkata S. Mattay, Andrew J. Saykin, Li Shen, Guillen Fernandez, Barbara Franke, Jing-Chun Chen, Xiang-Ning Chen, Jin-Kai Wang, Xiao Xiao, Xue-Bin Qi, Kun Xiang, Ying-Mei Peng, Xiang-Yu Cao, Yi Li, Xiao-Dong Shi, Lin Gan, Bing Su

Psychiatry Publications

One of the most significant evolutionary changes underlying the highly developed cognitive abilities of humans is the greatly enlarged brain volume. In addition to being far greater than in most other species, the volume of the human brain exhibits extensive variation and distinct sexual dimorphism in the general population. However, little is known about the genetic mechanisms underlying normal variation as well as the observed sex difference in human brain volume. Here we show that interleukin-3 (IL3) is strongly associated with brain volume variation in four genetically divergent populations. We identified a sequence polymorphism (rs31480) in the IL3 promoter which …

Preserved Ex Vivo Inflammatory Status In Decidual Cells From Women With Preterm Labor And Subclinical Intrauterine Infection, Violeta Castro-Leyva, Aurora Espejel-Nunez, Gerardo Barroso, Veronica Zaga-Clavellina, Arturo Flores-Pliego, Iyari Morales-Mendez, Silvia Giono-Cerezo, Scott W. Walsh, Guadalupe Estrada-Gutierrez

Obstetrics and Gynecology Publications

Objective

To compare the inflammatory response preserved ex vivo by decidual cells isolated from women who experienced preterm labor with and without subclinical intrauterine infection.

Methods

Fetal membranes were obtained after cesarean section from 35 women who delivered before 37 weeks of gestation following spontaneous preterm labor, with no clinical evidence of intrauterine infection. Decidua was microbiologically tested and cultured. Concentrations of anti-inflammatory cytokines (IL-2, IL-4, IL-10), pro-inflammatory cytokines (IL-6, IL-8, IL-1β and TNF-α), and matrix metalloproteinases (MMP-1, MMP-2, MMP-3, MMP-7, MMP-8, MMP-9) were measured in the supernatants using Bio-Plex, and prostaglandin E2 (PGE2) was measured by enzyme immunoassay.

Results …

Hemorrhage Detection And Segmentation In Traumatic Pelvic Injuries, Pavani Davuluri, Jie Wu, Yang Tang, Charles H. Cockrell, Kevin R. Ward, Kayvan Najarian, Rosalyn H. Hargraves

Electrical and Computer Engineering Publications

Automated hemorrhage detection and segmentation in traumatic pelvic injuries is vital for fast and accurate treatment decision making. Hemorrhage is the main cause of deaths in patients within first 24 hours after the injury. It is very time consuming for physicians to analyze all Computed Tomography (CT) images manually. As time is crucial in emergence medicine, analyzing medical images manually delays the decision-making process. Automated hemorrhage detection and segmentation can significantly help physicians to analyze these images and make fast and accurate decisions. Hemorrhage segmentation is a crucial step in the accurate diagnosis and treatment decision-making process. This paper presents …

A Comparison Of Two Distinct Murine Macrophage Gene Expression Profiles In Response To Leishmania Amazonensis Infection, Christian M. Probst, Rodrigo A. Silva, Juliana P.B. Menezes, Tais F. Almeida, Ivana N. Gomes, Andréia C. Dallabona, Luiz S. Ozaki, Gregory A. Buck, Daniel P. Pavoni, Marco A. Krieger, Patrícia S.T. Veras

Study of Biological Complexity Publications

Background

The experimental murine model of leishmaniasis has been widely used to characterize the immune response against Leishmania. CBA mice develop severe lesions, while C57BL/6 present small chronic lesions under L. amazonensis infection. Employing a transcriptomic approach combined with biological network analysis, the gene expression profiles of C57BL/6 and CBA macrophages, before and after L. amazonensis infection in vitro, were compared. These strains were selected due to their different degrees of susceptibility to this parasite.

Results

The genes expressed by C57BL/6 and CBA macrophages, before and after infection, differ greatly, both with respect to absolute number as well as …

Genome-Wide Association Study Heterogeneous Cohort Homogenization Via Subject Weight Knock-Down, Andre X. C. N. Valente, Joseph Zischkau, Joo H. Shin, Yuan Gao, Abhijit Sarkar

Study of Biological Complexity Publications

Population structure can be a source of both false-positive and false-negative findings in a genome-wide association study. This article proposes an approach that helps to reduce the false-positives. It consists of homogenizing the diseased/healthy phenotype ratio across the cohort, by decreasing the statistical weight of selected individuals. After homogenization, the cohort is statistically handled as if originating from a single well-mixed population. The method was applied to homogenize a Parkinson's disease genome-wide association study cohort.

Service-Learning In English Language Courses, Audrey Short, Susan Dudley, Lynn Pelco

Division of Community Engagement Resources

No abstract provided.