Digital Commons Network^™

Differential Reactivity Of Microglia In Two Mouse Models Of Multiple Sclerosis, Rebecca K. Hartley

Theses and Dissertations

Multiple sclerosis (MS) is a neurodegenerative disorder characterized by CNS inflammation and axonal demyelination. In addition, axonal pathology has also been reported in MS and may be responsible for the functional deficits associated with this disease. Based on preliminary data from our laboratory, we propose that a specific domain of the neuron, known as the axon initial segment (AIS), is targeted in MS. Consistent with our work from the human tissue, we have also observed disruption of AIS integrity in a murine CNS inflammatory model and observations strongly implicate reactive microglia as mediators of AIS disruption. In contrast, a murine …

Effects Of Traumatic Brain Injury On Oxycodone Reinstatement And Physical Dependence, Neil B. Varshneya

Theses and Dissertations

Epidemiological data indicate that patients who experience a traumatic brain injury (TBI) have an elevated risk of developing a substance use disorder (SUD), but the underlying neurobiological connections remain unclear. To further understand the relationship between TBI and SUD, we investigated the effects of TBI on the abuse-related effects of oxycodone in preclinical models. Our evaluation utilized a lateral fluid percussion injury of moderate severity in adult male Sprague-Dawley rats. In the first aim, we tested the hypothesis that moderate TBI increases the risk for relapse to an opioid use disorder as measured by reinstatement of lever-pressing behavior following extinction …

Resolution Of Inflammation Rescues Axon Initial Segment Disruption, Nicholas M. George

Theses and Dissertations

Axonal domains are required for proper neuron function. These domains are unstable and degenerate concurrent with the inflammation in multiple sclerosis (MS) and the inflammatory disease models experimental autoimmune encephalomyelitis (EAE) and lipopolysaccharide (LPS) induced inflammation. Previous studies from our laboratory have shown that the axon initial segment (AIS) is maintained independently of the presence of myelin, but that AIS disruption is seen in MS as well as EAE and LPS-mediated inflammation. AIS loss can be interrupted in the early stage of EAE using the anti-inflammatory drug Didox. However, the potential for Didox directed repair of the AIS in later …

Free & Content, Victoria M. Ahmadizadeh

Theses and Dissertations

I draw on my natural interest in my family’s eclectic collections – both traditional and personal – to create poetic portraits composed of carefully made and arranged objects. The goal of my portraiture is not to convey physical likeness; superseding the imitation of appearance is psychological portrayal of the desires and disappointments of the sitter. Often, a fragmented sense of self is revealed – both in others and in myself – and I seek to depict the kaleidoscopic nature of individuals in their given situations. Possessing whimsical, playful elements as well as encounters with lack and longing, I create work …

Behavioral Phenotyping Of Vmat1 Knockout Mice: Relevance To Neuropsychiatric Disorders, Kevin A. Webster Ph.D.

Theses and Dissertations

Schizophrenia is a debilitating mental disorder that causes a large economic burden and is prevalent across all cultures and countries around the world. Although both environmental factors and genetics are known to play an important role in the etiology of schizophrenia, the exact role of genetics and its interaction with environmental factors in an individual’s predisposition to develop schizophrenia is poorly understood. Schizophrenia is characterized by symptoms that include positive symptoms (e.g. delusions, hallucinations, disorganized thinking and speech), negative symptoms (e.g. avolition, anhedonia, depressive-like behavior), and cognitive dysfunctions (e.g. executive functioning deficits in learning and memory, attention, and vigilance). Genomic …

Identifying Profiles Of Resilience Among A High-Risk Adolescent Population, Anna W. Wright

Theses and Dissertations

The purpose of the present study was to determine whether distinct patterns of adolescent adjustment existed when four domains of functioning were considered. The study included a sample of 299 high-risk urban adolescents, predominantly African American, ages 9-16 and their maternal caregivers. Cluster analysis was used to identify patterns of adjustment. Logistic regression analyses were used to explore whether variations in levels of five theoretically and empirically supported protective factors predicted cluster membership. A four-cluster model was determined to best fit the data. Higher rates of goal directedness and anger regulation coping predicted membership within the highest functioning cluster over …

Loneliness, Cynical Hostility, And Cognitive Decline In Americans Above Age 50, Sarah C. Griffin

Theses and Dissertations

Background. Research identifies isolation (being alone) as a risk factor for cognitive decline— yet it is possible that subjective dimensions of isolation are more critical. Potential risk factors are loneliness (the distress stemming from feeling alone) and cynical hostility (an attitude of distrust and cynicism). The present study examined the relationship between these factors and cognitive functioning and decline.

Methods. Data came from the Health and Retirement Study, a nationally representative longitudinal study of US adults over 50. Loneliness was measured using the Hughes Loneliness Scale; cynical hostility was measured using items from the Cook-Medley Hostility Inventory. Cognitive functioning was …

Influence Of Historic Landscapes And Contemporary Species Management On Chesapeake Bay Bald Eagles And Osprey, Catherine B. Viverette

Theses and Dissertations

Influence of historic landscapes and contemporary species management on Chesapeake Bay Bald Eagles and Osprey

Catherine B. Viverette

Co-distributed species with well documented demographic histories can provide good models for testing alternative hypotheses about the impact of evolutionary history, contemporary landscapes, and species management on current distribution and population structure. The Osprey (Pandion haliaetus) and Bald Eagle (Haliaeetus leucocephalus) have been extensively studied, managed and monitored across their North American breeding range, particularly in the Chesapeake Bay. We used a combination of ecological niche modelling, diet reconstruction, and population genetic modeling to understand the role of …

Causes And Consequences Of Peer Victimization Among Adolescents With Autism, Sarah T. Doyle

Theses and Dissertations

A significant, yet understudied issue that demands attention is the experience of peer victimization among adolescents with autism spectrum disorders (ASDs). Research indicates that youth with disabilities, including those with ASDs, are victimized more frequently as compared to their typically developing peers. However, little is known about the peer victimization experience for adolescents with ASDs beyond its frequency of occurrence. This study examined relations between peer victimization and individual, peer, and parent factors and outcomes including internalizing and externalizing symptoms among adolescents with ASDs. No significant indirect effects were found for peer victimization on relations between individual social-cognitive and emotion …

The Transition From The Psychical To The Psychological: An Examination Of William James’ Influence On Henry James’ “The Turn Of The Screw”, Harry A. Jones Iv

Theses and Dissertations

This thesis will show that, in its original form, “The Turn of the Screw” acted as a monument to the intellectual unity shared between Henry James and his brother William. Through evaluating James’ biography, memoirs, and letters with William, this thesis will illustrate the subtle collaborative inspirations that initially helped James write the first twelve-part serial edition of “The Turn of the Screw” for Collier’s Weekly, which ran from January 27, 1898 until April 16, 1898. I will also demonstrate the effect of William’s philosophy and his death on the revisions James’ made to his story as published in the …

Sulfated Dehydropolymer Of Caffeic Acid For Repair Of Lung Damage And Emphysema, Tien M. Truong

Theses and Dissertations

The complex pathobiologic mechanisms of emphysema are not fully understood, leaving this deadly disease without effective pharmacotherapy for a cure. This project hypothesized that the sulfated dehydropolymer of caffeic acid (CDSO3) exhibits Fe²⁺ chelation-based hypoxia inducible factor-1a (HIF-1a) up-regulatory protective activities against in vitro emphysematous cell death and for in vivo reversal of emphysema induced with SU5416, a vascular endothelial growth factor blocker.

Using in vitro chromogenic competitive inhibition assays, CDSO3 was shown to chelate Fe²⁺ (IC₅₀ of 23 µM), but not Fe³⁺ ions. The trypan blue exclusion and lactate

dehydrogenase assays were then employed to …

Methcathinone Analogue Activity At The Human Serotonin Transporter, William Drake Varn

Theses and Dissertations

In the last few years, there has been continued concern about synthetic drug abuse in both the United States and worldwide. Small adjustments in drug compound structure often allow synthetic drug makers to manufacture a legal product that can produce the same highs as illegal counterparts. Unfortunately, this is happening faster than the government can outlaw the drug compounds, and a wide variety of synthetics are now appearing on the street. This study evaluated the effects on the human serotonin transporter of six different 4-para substituted methcathinone compounds. Using a Xenopus oocyte model, the efficacy of each MCAT analogue …

Examining Genetic And Environmental Influences On Alcohol Use And Externalizing Behaviors In African American Adolescents, Neeru Goyal

Theses and Dissertations

The aim of this thesis is to expand our understanding of the etiology of alcohol misuse and related disorders in African Americans using genetically informative study designs. Specifically, we take advantage of the candidate-gene approach and polygenic score analysis to extend the literature specific to African American populations. Chapter 2 explores gene x environment (GxE) interactions through the candidate gene approach to explore the relationship between two genes chosen on their potential relevance to stress response and adolescent alcohol use and misuse, among African American youth living in highly impoverished neighborhoods, as moderated by stressful life events. Chapter 3 implements …

An Extension Of Penalized Ordinal Response Models, Qing Zhou

Theses and Dissertations

Ordinal responses are commonly seen in medical research. Many pathological evaluations and health status outcomes are reported on an ordinal scales. Some examples of ordinal outcomes include cancer stage (I, II, III and IV), or stage of liver disease (normal liver, chronic hepatitis, cirrhosis and end of stage liver disease or hepatocellular carcinoma (HCC)).

In recent years, there has been a demand to understanding the pathogenic association between ordinal clinical outcomes and molecular characteristics. Genomic characteristics are often assayed using a high-dimensional platform where the number of interrogated sites (P) exceeds the number of samples (n). Unfortunately, traditional ordinal response …

Sweaty Mother Slow Groove, Devin Kylie Harclerode

Theses and Dissertations

Sweaty Mother Slow Groove is an engagement in magical thinking that proposes a displacement of swamp methodologies into the virtual realm, existing during the fourth wave. In doing so the cyborg and goddess are united in a re-routing of essentialism and the neo-liberal domination of technology. The metaphorical swamp is the possibility of a mushy danger zone that harnesses the absorption of an unwanted space: a disintegration of the binary and the soft-coded awareness of the body as a process, not a site.

No Woman Is An Island, Adele Ball

Theses and Dissertations

We have gathered the following pages to archive our time here in Richmond, Virginia. We have been here for two years, growing slowly, moving when needed to new anchorholds to avoid detection or arrest. We scrutinize the urban environment like modern archeologists. We collect stories and speculate about new uses of old things. It is imperative to be resourceful here, and we do so out of necessity but also in the spirit of practice. These pages were made en route, each an exploration of the tools at hand when on the move. The method of creation is just as important …

"Fuck Off, Get Free, Love And Love's The Only Thing", David S. Moré

Theses and Dissertations

A child, angry at concrete and strip malls, enjoying coffee from too many styrofoam cups.

Are You Covered? Examining How Knowledge Of The Patient Protection And Affordable Care Act Influences Use Of Preventive Reproductive Health Services, Ashlee Sawyer

Theses and Dissertations

The Patient Protection and Affordable Care Act (PPACA) expanded access to insurance coverage and health care services for many citizens, and has increased access for women in particular by including preventive reproductive health services as essential health benefits. The current national rates of sexually transmitted infections (STIs), reproductive cancer diagnoses, and unintended pregnancy serve as major areas of concern for women’s health and public health. The present study examined how knowledge of the PPACA influences receipt of preventive reproductive health services among women. Results indicate that higher levels of knowledge of the PPACA are associated with a greater likelihood of …

Death Vegas Valley, Eric M. Diehl

Theses and Dissertations

My work explores hallucinatory landscapes of the US American West by using a combination of painting styles outside of the Western painting canon. I cross-reference painting and cinema, interweaving video, acrylic paint and the panorama to create a satirical homage to the history and present state of the USA. However, through an earnest devotion to the medium - both painting and cinema - I find my criticisms also yearn to hold onto a belief in a myth I know to be false.

This is an American History conversation about artifice and consumerism through advertising. I use Las Vegas and the …

Telomere Length In Women With Breast Cancer: A Longitudinal Study Of Their Relationship To Chemotherapy And Acquired Psychoneurological Symptoms, Areej Alhareeri

Theses and Dissertations

Breast Cancer (BC) is one of the most commonly diagnosed malignancies in females. The 5-year survival rate for women with early stage BC is about 90%. However, the quality of life (QOL) for many of these women may be adversely affected due to treatment/cancer–related side effects, including a constellation of symptoms (anxiety, depression, pain, fatigue, sleep disturbance and depression), that are collectively termed psychoneurological symptoms (PNS). To gain insight into the contributory role of telomere length (TL) in the development and persistence of PNS, we have longitudinally studied 72 women (ages 23-71) with early stage BC (I-IIIA) at 5 time …

Momentum Clinic For Teen And Young Adult Cancer Patients, Merian O'Neil

Theses and Dissertations

This study explores the needs of teen and young adult cancer patients undergoing long-term chemotherapy treatments and the design of an outpatient clinic that accommodates this population’s unique needs. Most teens endure long-term treatments in a pediatric facility targeted primarily for children, while others are treated in adult facilities. Teens have different privacy and social needs than children, and while they are not adults, they are involved in their own treatment decisions, unlike children. The target age group for this study is teens and young adults, ages thirteen to twenty-two. A common theme emerged from research and interviews conducted in …

Sensible Nonsense, Timothy J. Woodbrey

Theses and Dissertations

“Truth happens only by establishing itself in the strife and the free space opened up by truth itself. Because truth is the opposition of clearing and concealing, there belongs to it what is here called establishing.”[i]—Martin Heidegger

All things contain their own individual existence. When I look at objects I notice material, tradition, individual history. These are starting points for my imagination to seed and germinate. My ideas are fragmented, nonlinear and nonsensical to others but they hold honesty to me. Honesty is powerful and is worth sharing.

This document is an examination of my work during …

Perceptions Of The Homeless Toward Nonprofit Human Service Provider, Lequan M. Hylton

Theses and Dissertations

As the debate intensifies regarding developing remedies to meet the needs of America’s homeless, one solution is for governmental agencies to collaborate with and employ organizations from the nonprofit sector to assist with the needs of the homeless population. Included in the nonprofit sector, faith-based organizations (FBOs) have historically been a source of debate and contention in terms of collaborations with the government. However, Presidents Reagan, George H. Bush, Clinton, George W. Bush, and Obama have embraced the idea of including FBOs in the pool of service providers offering human services. In the Richmond, Virginia region, FBOs and nonreligious nonprofit …

Quantitative Analysis Of 5-Chloro-2-Methoxy-N-[2-(4-Sulfamoylphenyl)Ethyl]Benzamide (Glyburide Analogue, Ga) In Mouse Plasma And Whole Blood Using A Micro-Extraction And Liquid Chromatography-Tandem Mass Spectrometry, Ankit Zalavadia

Theses and Dissertations

Pharmacokinetic evaluation of 5-chloro-2-methoxy-N-[2-(4- sulfamoylphenyl)ethyl]benzamide in mouse plasma demanded for a suitable bioanalytical method. No reported bioanalytical method exists to-date that can quantify concentration of this compound in any biological matrix. The purpose of this study was 1) to develop and validate a new bioanalytical method using a micro-extraction and LC-MS/MS to quantify the target analyte in mouse plasma and 2) to partially validate the method in whole blood. A bioanalytical method was developed and validated in both matrices for a linear concentration range of 2-1000 ng/ml. For both matrices, the reverse predicted concentration of calibration standards (-8.95% to 12.16% …

Conditional Cardiac-Specific Akap13 Knockout Induces Sex Dependent Biventricular Dilated Cardiomyopathy With Sarcomeric And Mitochondrial Defects, Kimberlyn M. Baig-Ward

Theses and Dissertations

Heart disease is a complex and heterogeneous disease. Notably, studies have demonstrated gender differences in the expression and types of cardiovascular disease, such as dilated cardiomyopathy (DCM), a major underlying cause of heart failure. Previously we showed that loss of A-Kinase Anchoring Protein 13 (Akap13), a unique proto-oncogene and estrogen receptor modulator, resulted in enlarged embryonic hearts, defective cardiac sarcomere formation, and embryonic lethality in mice. Data have also shown cAMP-dependent Protein Kinase A (PKA) to be involved in DCM pathophysiology. Given the established role of AKAP13 in cell signaling, its ability to bind and modulate ligand-activated nuclear hormone …

Exploring The Outcomes Of Rehabilitative Care For Veterans And Service Members Treated For A Disorder Of Consciousness In The Vha Emerging Conciousness Program, Janette A. Hamilton

Theses and Dissertations

Over the past several years, there has been an influx in patients being treated for polytraumatic injuries within the Veterans Health Administration (VHA), largely due to the wars in Afghanistan and Iraq, but also due to advances in life sustaining medical interventions. The polytrauma population includes veterans who have sustained a severe traumatic or non-traumatic brain injury, and a significant loss in cognitive and physical functioning, referred to as a disorder of consciousness. The purpose of the current study was to explore factors related to successful emergence from a disorder of consciousness, using a sample of veterans who were treated …

Response Adaptive Randomization Using Surrogate And Primary Endpoints, Hui Wang

Theses and Dissertations

In recent years, adaptive designs in clinical trials have been attractive due to their efficiency and flexibility. Response adaptive randomization procedures in phase II or III clinical trials are proposed to appeal ethical concerns by skewing the probability of patient assignments based on the responses obtained thus far, so that more patients will be assigned to a superior treatment group. General response-adaptive randomizations usually assume that the primary endpoint can be obtained quickly after the treatment. However, in real clinical trials, the primary outcome is delayed, making it unusable for adaptation. Therefore, we utilize surrogate and primary endpoints simultaneously to …

Catching Up And Staying Out Of Trouble: Serious Juvenile Offenders’ Facility School Experiences And Their Transition To The Community, Lena Jäggi

Theses and Dissertations

Despite recent drops in rates, juvenile incarceration remains a serious issue in the United States (Hockenberry, 2013; Mendel, 2011). One shared part of the incarceration experience across different systems and facility types is the obligation for juvenile offenders to receive correctional education. Ample research demonstrates that increased academic achievement, attending community school, and being employed are connected to better community outcomes and desistance, yet little is known about how school experiences in the facility influences these outcomes. Applying life-course theory of the development of crime (Sampson & Laub, 1997, 2005), the present study investigates whether correctional education serves as a …

Capturing Memory, Morgan A. Molenda

Theses and Dissertations

In my work, I present the moments of true emotional clarity and impactful memories from my life. I strive to provide insight into my experiences and my understanding of others’ motivations. The creative process helps safely guide me through memories of the past and understanding of others.

In my abstracted mixed media paintings and crafts, I use bold jewel tones to express strong feelings and passionate emotions. I also incorporate found objects and paints to communicate the layers and varied depth of memories. With the use of these varied materials, I have developed a personal symbolic language that allows me …

Evaluating A Brief Web-Based Prevention Intervention For Risky Alcohol Use Among College Students, Zoe Neale

Theses and Dissertations

The purpose of this study was to evaluate a brief, web-based alcohol prevention intervention program as a universal approach to addressing the range of alcohol behaviors present on college campuses. The sample of freshman college students recruited from Spit for Science (Dick et al., 2014) included 153 intervention participants, and 151 control participants matched on demographics and baseline alcohol variables. Hierarchical multiple regression, logistic regression, and moderated multiple regression were used to compare intervention and control participants on post-intervention alcohol variables. Treatment predicted lower alcohol use disorder (AUD) symptoms, particularly among baseline drinkers. For non-drinkers, the intervention was associated with …