Digital Commons Network^™

Theta Burst Brain Stimulation In Painful Diabetic Neuropathy Patients: Investigating Neural Mechanisms, Bhushan Thakkar

Theses and Dissertations

Chronic pain (CP) is a significant contributor to disability and disease burden globally. In 2019, approximately 50.2 million adults (20.4% of the US population) experienced chronic pain, contributing to $560-635 billion in direct medical costs. In addition, the worldwide prevalence of diabetes mellitus has reached epidemic proportions and is set to increase to 629 million by 2045. Almost 50% of patients with diabetes present with diabetic neuropathy (DN), and one in five patients with diabetes presents with painful DN (pDN) which is the most common cause of neuropathic pain (NP) in the US. Symptomatic treatment is the mainstay of management …

Anthracyclines Attenuate The Nrf1-Mediated Bounce-Back Response, Bader Albalawi

Theses and Dissertations

Proteasome inhibitors, such as carfilzomib, are FDA-approved to treat multiple myeloma and mantle cell lymphoma. Unfortunately, proteasome inhibitors have only produced clinically significant results in patients with hematologic cancers, despite their predicted pan-cancer utility, and even hematologic cancer types frequently show intrinsic and acquired resistance.

One proposed mechanism responsible for the proteasome inhibitors' shortcomings is the NRF1-mediated bounce-back response. Identification of drugs that can potentiate the action of proteasome inhibitors could overcome resistance in patients with hematologic cancers and expand proteasome inhibitors' use to treat solid tumors. Our previous studies have identified anthracyclines as potential compounds that interfere with the …

Examining Ayahuasca Constituents At 5-Ht2a Receptors In Search Of Antidepressant Action, Jeremy D. Rolquin

Theses and Dissertations

Recently, it has been reported that ligands of the 5-HT_2A receptor can have drastic and fast-acting efficacy towards a number of different mental health disorders, such as depression and anxiety. A mixture of Amazonian plants calledayahuasca contains multiple compounds which have been shown to interact with the serotonergic system, and the 5-HT_2Areceptor in particular.

The structurally similar compounds (DMT, harmine, harmaline, and tetrahydroharmine) found in ayahuasca were examined for differences in their physiochemical properties that might contribute to their binding affinity for the 5-HT_2Areceptor. 3D Molecular modeling and docking studies of these compounds were …

The Role Of Human Dopamine Transporter Threonine 261 And Serine 262 In Amphetamine Regulation Of Transporter Function And Surface Expression, Sina Abdollahi

Theses and Dissertations

Amphetamine (AMPH) has been used clinically and recreationally around the world for years. A 2021 study reports a 67.5% rise in AMPH use from 2010-2017 across all fifty states. Study also shows that 30% of patients with AMPH-induced psychosis end up with primary psychosis over time. AMPH works by inhibiting monoamine (Norepinephrine (NE), Serotonin (5-HT), and Dopamine (DA) reuptake followed by triggering transporter mediated monoamine release and consequently increases monoamine concentrations in extracellular synaptic space. AMPH also works as a substrate for monoamine transporters, enters the neuron, and once its inside the neuron, it will prevent the storage of monoamines …

Identification Of The New Synthetic Opioid Bucinnazine And Its Metabolites Using Liquid Chromatography Tandem Mass Spectrometry (Lc-Ms/Ms), Karissa N. Resnik

Master of Science in Forensic Science Directed Research Projects

In the past 20 years, the rate of opioid use increased drastically in the United States. The country has experienced an enormous number of deaths caused by opioid overdose, opioid use disorder (OUD), as well as other harms as a consequence of the high numbers of opioid prescriptions. Fentanyl, a well-known synthetic opioid, has been a contributor to the opioid crisis since 2013 due to its popularity in clinical and illicit use. It has a high potency, which makes it an effective analgesic but a dangerous illicit substance. A new synthetic opioid, bucinnazine, has recently become a new black-market opioid …

Optimization Of A Microfluidic Device For Cell Capture Using Optical Trapping, Samantha C. Pagel

Master of Science in Forensic Science Directed Research Projects

Biological mixtures which result from evidence produced in sexual assault cases are one of the most common types of mixtures observed in forensic biology casework and have contributed to evidence backlogs across the country. In the forensic community, this has highlighted the need for a more efficient cell separation method at the beginning of the DNA analysis workflow than the currently accepted method. One promising novel front-end cell separation technique is optical trapping. In heterogeneous mixtures, optical tweezers have been demonstrated to manipulate and isolate cells of interest from other cell types.

For this research, a range of 5 to …

Bowling Green Main Street Revitalization Plan, Amy Guzulaitis

Master of Urban and Regional Planning Capstone Projects

The Town of Bowling Green is a historic, rural community located in Caroline County, Virginia. The Town is the pride of residents who enjoy the community and small town nature of the area. The Town currently has a small arts business cluster and sought ways to use that, as well as the Main Street Method, as a basis for spurring revitalization in the Town. Research Methods & Findings Today, the Town of Bowling Green faces challenges to its future growth and development due in part to the small market size available. Demographic indicators from ESRI Business Analyst show that Bowling …

Growing Small: Citizen Preferences For New Development In Highland County, Virginia, And The Town Of Monterey, Charles F. Wilson

Master of Urban and Regional Planning Capstone Projects

“Growing Small: Citizen Preferences for New Development in Highland County, Virginia and the Town of Monterey” is a plan by Charles Wilson for Highland County (“Highland”) as they reconsider the future of the Town of Monterey (“Monterey”). Highland is a rural county in western Virginia at the intersection of U.S. Routes 250 and 220. In the Summer of 2021, a new Family Dollar was issued permits to demolish an historical lodging facility and construct a new store—just a few buildings away from an existing Dollar General. This was not without controversy, but ultimately prompted reflection amongst community members for how …

Outcome Of Vital Pulp Therapy On Immature Permanent Teeth With Calcium Silicate-Based Materials: Retrospective Cohort Study, Brian I. Hone

Theses and Dissertations

Introduction: The purpose of vital pulp therapy is to maintain pulp viability by eliminating bacteria from the dentin-pulp complex and to establish an environment in which apexogenesis can occur. The aim of this retrospective cohort study was to evaluate the clinical and radiographic outcomes of vital pulp therapy on immature permanent teeth using different calcium silicate-based materials. Methods: This study was carried out in the Virginia Commonwealth University Graduate Endodontics department in Richmond, Virginia. Vital pulp therapy cases performed by graduate endodontic residents were retrospectively searched and charts were reviewed to determine inclusion into this study. All cases …

Shenandoah Valley Partnership: Growing And Enhancing The Food And Beverage Manufacturing Industry, Justin Williams

Master of Urban and Regional Planning Capstone Projects

This plan analyzes how the Shenandoah Valley Partnership can grow and enhance its food and beverage manufacturing industry. To answer this question, I interviewed and surveyed food and beverage manufacturers to understand their business needs, as well as interviewed service providers to identify their relationships with this industry. The four main results from the plan were:

• There is a need for more capital for the businesses.

• There is a labor shortage in the industry.

• The Shenandoah Valley has an excellent distribution location.

• The industry has a poor relationship with the region’s service providers.

The plan outlines recommendations on how institutions …

Richmond Community Compost Initiative Plan, Brendan Mcdowell

Master of Urban and Regional Planning Capstone Projects

The City of Richmond is beginning the process of creating a composting program for the first time in the city’s history. It is part of a cross-departmental collaborative plan to divert food waste from the landfill and into a new composting program. The Office of sustainability is in the process of drafting the RVAGreen 2050 Climate and Equity Master Plan for Richmond, which includes an ambitious zero-waste goal. In order to accomplish this future goal, the city must begin setting up the infrastructure to begin diverting waste from the landfill.

The purpose of the Community Composting Initiative Plan is to …

Active To Reserves, Patrick Potter

Mighty Pen Project Anthology & Archive

An army veteran decides to return to the military via the reserves. He finds that things are quite different than he expected.

Articles, stories, and other compositions in this archive were written by participants in the Mighty Pen Project. The program, developed by author David L. Robbins, and in partnership with Virginia Commonwealth University and the Virginia War Memorial in Richmond, Virginia, offers veterans and their family members a customized twelve-week writing class, free of charge. The program encourages, supports, and assists participants in sharing their stories and experiences of military experience so both writer and audience may benefit.

The Transcription Factor Nfe2l2a Is Required For Development Of Hematopoietic Stem Cells In The Zebrafish Embryo, Sivam Bhatt, Teerth Patel, Madeleine Seputro, Anubhav Thapaliya, Erich Damm

UROP Posters

Hematopoietic Stem Cells (HSCs) are the self-renewing population of cells that generate all erythrocytes and leukocytes over the lifetime of a vertebrate organism. HSCs are also the therapeutic units of curative bone marrow transplants used in the treatment of blood malignancies and in gene therapy for genetic blood disorders. In all vertebrate embryos, HSCs originate from the floor of the embryonic dorsal aorta during the endothelial to hematopoietic transition. Nascent HSCs will bud into the blood vessel and be carried to maturation sites by the embryonic blood flow. Despite the curative potential of HSC transplants in blood disorders, this approach …

A Personal History Of The Department Of Biology, Margaret L. May

VCU University History Books

Former faculty member Margaret May recounts the history and development of the Department of Biology at Virginia Commonwealth University.

Mechanisms And Treatment Of Pain-Depressed Behavior In Male And Female Mice, Clare M. Diester

Theses and Dissertations

One of the largest discrepancies between preclinical and clinical assessment of pain and analgesia is the type of behavioral endpoint used. Although most preclinical research has historically focused on drug effectiveness to block reflexive withdrawal behaviors stimulated by a noxious stimulus, increasing evidence shows that drug-induced restoration of pain-depressed behaviors provides increased clinical translation with decreased susceptibility to false-positives. Accordingly, the first portion of this dissertation designed a behavioral battery that featured pain-depressed behaviors as well as more conventional pain-stimulated behaviors for testing candidate analgesics in male and female mice. The main findings are as follows. (1) Intraperitoneal injection of …

Therapeutic Approaches For Respiratory Distress Syndrome, Franck J. Kamga Gninzeko

Theses and Dissertations

Respiratory distress syndrome (RDS) is characterized by shortness of breath and low oxygen levels. RDS affects the neonatal and adult populations. In the neonatal population, RDS can be classified as NRDS (Neonatal respiratory distress syndrome), while in adults, it is known as ARDS (acute respiratory distress syndrome). This dissertation examines a therapeutic approach to NRDS and a mechanistic approach to ARDS with in vivo and in vitro models of lung injury. NRDS is characterized by a deficiency or lack of surfactant. Surfactant is an essential compound composed of phospholipids and proteins to prevent the lungs from collapsing. There are several …

Race, Weight, Gender And The Embodied (Odied, Odied) Consciousness Of Big-Bodied Black Women Educators: A Phenomenological Study, Kendra D. Johnson

Theses and Dissertations

Big-Bodied Black women in the United States have perpetually navigated the veritable dichotomy of being hyper-visible and invisible (Beauboeuf-Lafontant, 2003; Fleetwood, 2001; Strings, 2019). Fat Black female bodies have borne the burden of exaggerated tropes and exploitation throughout history, stripping them of their femininity and humanity and resulting in a unique form of objectification (Strings, 2019). Inconsistent messages about BBWs, their bodies, and their value in society have endured for generations. They have been essential in constructing the controlling images of Black womanhood in the U.S. (Collins, 2000). The controlling images all evoked thoughts about the suitability of Black women …

Roots And Webs And Nets And Branches And Bulletin Boards And Banners And Newsletters And Mutual Aid Text Threads And Kin And Caretakers And Porches And Poems Of Today And Spaces Of Survival, Lukaza Branfman-Verissimo

Theses and Dissertations

As I welcome Richmond, VA into my family, I find myself needing to make roots and webs and nets and branches that ground me, that place myself as a Black, queer, mixed race, artist, activist, educator, storyteller, and cultural worker in this city. I am called to the streets before I am called to my studio. I question what it means to be a part of an institution that is slowly eating this city up. I become a story collector. I need to know where I am and whose land I now call home.

Blast Zone, Eleanor M. Thorp

Theses and Dissertations

My works emphasize the animistic qualities of extracted or displaced stones. This paper will explore my practice in relationship to practices of image reading, and drawing images within images, particularly rocks. I will use traditions of landscape painting, geological study, and Persian image making to talk about displacement of materials and stones.. I will explain how viewers can be trapped inside of the rock and my use of pastels, rock mediums, colored pencils, and my fingers to build these rocky landscapes.

Graduate School's Transformative Awakening: An Arts-Based Autoethnography, Roxanne L. Brown

Theses and Dissertations

This is an autoethnographic journey of a 30-year veteran art teacher through graduate school during the 2016 presidential campaign, election, presidency, #MeToo movement, a global pandemic, Black Lives Matter (BLM), virtual teaching and grading during a pandemic. My narrative includes an account of my transformative academic, social, and personal experiences and how they have informed my teaching practice. They include a collection of visual representations I created during my graduate school journey.

The Abuse Liability Profile Of An Unflavored, Sucralose-Sweetened, Electronic Cigarette In Combustible Cigarette Smokers, Sarah F. Maloney

Theses and Dissertations

The purpose of this study was to examine the effects of sucralose and nicotine in otherwise unflavored PG/VG ECIG liquid solutions, via a 30-watt ECIG, using multiple measures predictive of abuse liability to provide a basic understanding of the effects of sweeteners and perceived sweetness on ECIG abuse liability in reference to combustible cigarettes. Fourteen dependent smokers completed five independent study sessions that were within-subject, Latin-square ordered, and were identical in regards to all aspects but the product used. In each session, participants were given either their own brand of cigarettes or one of the four ECIG conditions: 0 mg/mL …

Peri-Implantitis Incidence And Effect Of Supportive Periodontal Therapy On Outcomes: Retrospective Cohort Study, Michael Ha

Theses and Dissertations

Purpose: Management of peri-implantitis is becoming an increasing component of dental practice. Dental implants can develop peri-implantitis lesions which occur in a non‐linear and accelerating pattern compared to a gradual periodontitis lesion. It is crucial to diagnose and treat this disease early, or ideally prevent it. Contemporary implants have a rough surface texture which is difficult to properly decontaminate as a crucial component for treatment prognosis. Evidence on the long-term effects of Periodontal implant maintenance therapy (PIMT) on implants is limited. This study aims to investigate the effect of periodontal maintenance on peri-implant tissue health.

Methods: A retrospective chart review …

Improving College Students’ Views And Beliefs Relative To Mathematics: A Systematic Literature Review Followed By A Multiple Case Mixed Methods Exploration Of The Experiences That Underpin Community College Students’ Attitudes, Self-Efficacy, And Values In Mathematics, Marquita H. Sea

Theses and Dissertations

Mathematics is particularly important due to its relevance in our daily lives. It is a general requirement throughout schooling. Unfortunately, many students openly declare negative views/beliefs regarding math in their personal and academic lives. These in turn, negatively influence students’ achievement related behaviors and outcomes. First, a systematic literature review was conducted to determine what types of studies/initiatives have aimed to enhance students’ views/beliefs relative to mathematics, including domain general and specific perceptions of math as well as their judgements of who is successful in mathematics and if they themselves can be successful. Specifically, the review centered on the components …

Puddlefist, Dylan Ahern

Theses and Dissertations

This written thesis focuses on my MFA thesis exhibition, Puddlefist. It begins with an introduction to both the artworks and my artistic approach, then discusses the text Transcendental Black Metal by Hunter Hunt-Hendrix, which serves as a larger reference point for the work. The latter part of this written thesis is a narrative influenced by the convergence of the sculptures that comprise the exhibition. I consider it a form of endurance writing that exercises motifs of noise, mantra, and monotony. In the section entitled Puc and Yellow, NPC (non-playable character) dialogue from the video game Fallout: New Vegas is transcribed …

Cadent Diffusion: Permeating The Membrane, Isabella M. Kubo

Theses and Dissertations

cadent diffusion: permeating the membrane explores and documents KUBO's journey of cultivating a sustainable and curious artistic practice during their Master’s program in Richmond, Virginia (Powhatan Land) from the Fall of 2020 to the Spring of 2022.

KUBO's practice is the affirmation between life and change in an attempt to work along the forces of singularity; to free lines, scores, concepts, and events from structures that otherwise bind them.The cadent diffusion is the rhythm in this force. Or perhaps, it is the force itself.

Wet, Flowering, Dry, Caroline A. Minchew

Theses and Dissertations

Wet, flowering, dry is a series of photographic works that explore how vernal pools are a macrocosm for holding memory and a site of omnipresent solitude and decay. This installation distills an embodied and ephemeral experience of how we are grounded in a network of invisible connections with our surroundings. This network becomes evident through biological, historical, and field research conducted at the vernal pools for over a year. Through slow observation and consideration of how multiple stories of place can weave together into a larger parable, Wet, flowering, dry reveals how the life cycle of a vernal pool is …

Until Its Calmness Can Claim You, Gabrielle Mchugh

Theses and Dissertations

This is an invitation to pause //

This is an externalization of my inner landscape, a highlight of what I value in my everyday and what comprises my lexicon of a sacred space. The following is a journey of nets, quiet, the sacred, space, and the in-between; where I share research and questions that are the foundation for my thesis work, Until Its calmness can claim you.

// This is an invitation to find moments of quiet in the noise

Traumatic Brain Injury-Induced Changes Of Immune/Inflammatory Response In The Context Of Predisposition Of Alzheimer’S Disease, Alexandra Pedin

Theses and Dissertations

Alzheimer’s Disease (AD) is a progressive neurogenerative disease that affects millions globally. At this time there are no effective treatment options as the primary cause for AD is not fully understood. In addition to old age, genetic predisposition, and certain health conditions, traumatic brain injury (TBI) has been identified as a risk factor for AD. While the pathway that links TBI to AD is still largely unknown, the neuroinflammatory response to both conditions has been connected to the NLRP3 inflammasome. Due to its role in the innate immune system and proinflammatory cytokine mediation, the NLRP3 inflammasome could be a potential …

Dregs / Lessons From The Things Around Us, Juan-Manuel Pinzon

Theses and Dissertations

The writing in Lessons From the Things Around Us is in support of the work in my MFA thesis show, dregs. I detail the progression of my making and thinking over the last two years. I expand on the material and personal relationships that have manifested themselves in the work and influenced my approach to the things that surround me. Finally, I point to a more expansive definition of Craft, rooted in its material sensibilities, and the possibilities already present in the field that this definition creates.

Defiantly Childlike: Using Aesthetic Resistance To Heal, Sarah K. Reagan

Theses and Dissertations

This thesis examines an alternative processing mechanism surrounding the act of healing after traumatic experiences in life. Using a methodology of iterative patterning and tool-pathing, a collection of inflatable garments and wooden mannequins analyzes defense mechanisms learned in early childhood development. This work highlights an essential body of recent scholarship that takes cuteification seriously to restore a childlike approach to mastering fear. This paper will review the definitions of cuteness and childlike humor and then describe how visual culture has implemented these components to subvert established power.