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Virginia Commonwealth University Undergraduate Bulletin Courses [2010], Virginia Commonwealth University

VCU Bulletins

Listing of undergraduate courses for 2010-2011.

Putative Cis-Regulatory Elements Associated With Heat Shock Genes Activated During Excystation Of Cryptosporidium Parvum, Benjamin Cohn, Patricio A. Manque, Ana M. Lara, Myrna G. Serrano, Nihar U. Sheth, Gregory A. Buck

Microbiology and Immunology Publications

Abstract

Background

Cryptosporidiosis is a ubiquitous infectious disease, caused by the protozoan parasitesCryptosporidium hominis and C. parvum, leading to acute, persistent and chronic diarrhea worldwide. Although the complications of this disease can be serious, even fatal, in immunocompromised patients of any age, they have also been found to lead to long term effects, including growth inhibition and impaired cognitive development, in infected immunocompetent children. The Cryptosporidium life cycle alternates between a dormant stage, the oocyst, and a highly replicative phase that includes both asexual vegetative stages as well as sexual stages, implying fine genetic regulatory mechanisms. The …

Traumatic Brain Injury And The Effects Of Diazepam, Diltiazem, And Mk-801 On Gaba-A Receptor Subunit Expression In Rat Hippocampus, Cynthia J. Gibson, Rebecca C. Meyer, Robert J. Hamm

Psychology Publications

Background

Excitatory amino acid release and subsequent biochemical cascades following traumatic brain injury (TBI) have been well documented, especially glutamate-related excitotoxicity. The effects of TBI on the essential functions of inhibitory GABA-A receptors, however, are poorly understood.

Methods

We used Western blot procedures to test whether in vivo TBI in rat altered the protein expression of hippocampal GABA-A receptor subunits α1, α2, α3, α5, β3, and γ2 at 3 h, 6 h, 24 h, and 7 days post-injuy. We then used pre-injury injections of MK-801 to block calcium influx through the NMDA receptor, diltiazem to block L-type voltage-gated calcium influx, …

Virginia Dental Journal (Vol. 87, No. 4, 2010)

Virginia Dental Journal

No abstract provided.

Immigration And Domestic Politics In South Africa: Contradictions Of The Rainbow Nation, Vernon D. Johnson

Ethnic Studies Review

The region of Southern Africa has been part of the global capitalist system since its inception in the late 15th century, when Portugal incorporated Angola and Mozambique into its empire. In 1652 the Dutch East India Company established a "refreshment station" at the Cape of Good Hope for ships travelling between Europe and the Far East.1 From that time the region has experienced several periods of deepening incorporation into the global system.

Long-Term Fish Oil Supplementation Induces Cardiac Electrical Remodeling By Changing Channel Protein Expression In The Rabbit Model, Xulin Xu, Min Jiang, Yuhong Wang, Timothy Smith, Clive M. Baumgarten, Mark A. Wood, Gea-Ny Tseng

Physiology and Biophysics Publications and Presentations

Clinical trials and epidemiological studies have suggested that dietary fish oil (FO) supplementation can provide an anti-arrhythmic benefit in some patient populations. The underlying mechanisms are not entirely clear. We wanted to understand how FO supplementation (for 4 weeks) affected the action potential configuration/duration of ventricular myocytes, and the ionic mechanism(s)/molecular basis for these effects. The experiments were conducted on adult rabbits, a widely used animal model for cardiac electrophysiology and pathophysiology. We used gas chromatography - mass spectroscopy to confirm that FO feeding produced a marked increase in the content of n-3 polyunsaturated fatty acids in the phospholipids of …

Poictesme: A Student Anthology Of Literature And Art (2010)

Pwatem (Poictesme), 2006-

Poictesme (now Pwatem) is an anthology of literature and art from undergraduate students at Virginia Commonwealth University. It publishes poetry, prose and art of all kinds from talented undergraduate students of all majors.

The Promiscuity Of Aesthetics, Paul Duncum

Journal of Social Theory in Art Education

I contend that the concept of aesthetics lies at the very heart of the art educational enterprise, albeit significantly reconfigured. I begin by offering a highly potted, historical overview of aesthetics that while it supports Tavin’s view of aesthetics as a confused and confusing concept, demonstrates how important it remains. My intention is not to support aesthetics as part of a progressive socio-political agenda, as many art educators do, but because the word aesthetics is today used extensively beyond our specialized area of art education to conceptualize the sensuousness of contemporary cultural forms. A brief investigation of books and articles …

Restageactivist Art/Disruptive Technologies, Karen Keifer-Boyd

Journal of Social Theory in Art Education

In this article, I explore, with you, artists’ socio-political disruptions with communication technologies to inspire political action and social change, and how such art can be environmentally and socially useful. How does art function politically? What is activist art? What non-violent forms of dissent or disruptions to harmful practices are possible today with digital technologies, and how do artists manifest political perspectives in their practice?

Casino Capers: Exploring The Aesthetics Of Superfluidity, Mary Stokrocki, Bianne Castillo, Michael Delahunt, Laurie Eldridge, Martin Koreck

Journal of Social Theory in Art Education

Casinos are fast becoming sites for display of new Native American (NA) Arts. In such a context, casinos re-represent themselves and their communities through various visual forms and thus change their meanings. In her study of Wisconsin casinos, Stuhr (2004) challenged art educators to consider these visual culture displays as they accommodate new markets. Art in the casino phenomenon is worth investigating and how art educators can explore and/or make sense of this phenomenon is important. Casinos are using artworks as spectacles of pleasure. According to a casino gambling survey conducted by Harrah’s Entertainment, approximately 40 million Americans played slot …

Ethnic Studies Review

Ethnic Studies Review

No abstract provided.

Obstructing The View: An Argument For The Use Of Obstructions In Art Education Pedagogy, Ryan Patton

Journal of Social Theory in Art Education

Through the use of obstructions we can see how a project-based curriculum can promote very different results. The obstructions that Sandy Skoglund gave the colloquium class at Ohio State were not presented as opportunities for play. Although Bickley-Green and Phillips allowed for play in their use of obstructions, the type of play described was prescriptive and limiting. Pitri’s use of play as a form of problem solving that also allows for personal expression advocated in this paper. Clearly identifying obstructions as game-like challenges for students, they can be used for growth and critical awareness.

The Effects Of Pet Ownership On Physical Well-Being In Older Adults, Tami Pohnert

Theses and Dissertations

A large percentage of Americans own pets which may impact their health. This study examines pet ownership’s effect on well being in older adults looking at race/ethnicity. A sample of 6,565 older adults (>60) was selected from the Third National Health and Nutrition Survey. Pet owners comprised 28.3% of the sample. The theories of symbolic interaction and social integration were used to examine pet ownership’s effect on physical components of well-being. The descriptive results showed statistically significant differences in age, education, income, and marital status between pet owners and non-pet owners. Pet owners were younger, more educate, higher income …

The Role Of The Ca2+-Dependent Protein Kinase, Camk-Ii, In Heart And Kidney Development In The Zebrafish, Danio Rerio, Sarah Rothschild

Theses and Dissertations

Ca2+/calmodulin-dependent protein kinase type II (CaMK-II) is a multifunctional serine/threonine kinase that is ubiquitously expressed throughout the lifespan of metazoans. Mammals encode four genes (α, β, γ, δ) that generate over forty splice-variants. CaMK-II is important in a myriad of functions, including ion channel regulation, cell-cycle progression, and long term potentiation. In adults, alterations in activation of CaMK-II induce cardiac arrhythmias and heart failure. Developmental roles for CaMK-II are not as well understood since mouse knockouts are embryonic lethal. Therefore the identification of other vertebrate CaMK-II genes will add to our understanding of development. Zebrafish encode seven catalytically active CaMK-II …

Role Of Oxidative Reactive Species And Antioxidants In Metabolism And Transport Of Therapeutic Drugs, Svetlana Verenich

Theses and Dissertations

Oxidative stress (OS) is a frequent complication of various disease conditions such as Alzheimer’s and Parkinson’s disease, atherosclerosis, preeclampsia, rheumatoid arthritis, diabetes including gestational diabetes, etc. OS is defined as an imbalance between the production of reactive species and the ability of an organism to detoxify the reactive intermediates and repair the damage. As a result of OS, the excess of reactive species such as oxygen superoxide (O2-), hydroxyl radical (OH), peroxynitrite (ONOO−), 4-hydroxynonenal (4HNE), etc., have a tendency to react with nearby proteins/nucleic acids/lipids changing their functionality or inactivating them completely. The organism has many ways to protect itself …

Autotaxin: A Regulator Of Oligodendrocyte Differentiation, Larra Yuelling

Theses and Dissertations

In order for oligodendrocyte progenitor cells (OPCs) to differentiate into fully mature, myelinating oligodendrocytes, they must be specified at the correct times and undergo coordinated changes in both gene expression and morphology. As oligodendrocytes differentiate, they transition from a bipolar morphology into a morphology characterized by a complex network of multiple processes, which will eventually generate membranous structures necessary for myelination of axonal segments. As changes are observed in cellular morphology, oligodendrocytes also undergo changes in their gene expression profile and express genes necessary for both early and later stages of development such as olig1 and myelin basic protein (mbp), …

And Her Increase And Selected Stories, Catherine Hart

Theses and Dissertations

A recently freed slave strives for her independence and renegotiates her relationship with the white father of her children in the aftermath of the Civil War. Foreclosed homeowners find a literally explosive artifact. Co-workers cope with layoffs and a suicide. A fugitive witch struggles against a fantasy theocracy. A revolutionary tries to save an innocent man and her conscience. A doctor chooses to resist an oppressive regime in a dystopian future. The historical novel And Her Increase and these widely varying stories coalesce around themes of freedom and oppression, choice and desire.

Prediction Of Human Systemic, Biologically Relevant Pharmacokinetic (Pk) Properties Based On Quantitative Structure Pharmacokinetic Relationships (Qspkr) And Interspecies Pharmacokinetic Allometric Scaling (Pk-As), Prajakta Badri

Theses and Dissertations

This research developed validated QSPKR and PK-AS models for predicting human systemic PK properties of three, preselected, pharmacological classes of drugs, namely opioids, β-adrenergic receptor ligands (β-ARL) and β-lactam antibiotics (β-LAs) using pertinent human and animal systemic PK properties (fu,, CLtot, Vdss, fe) and their biologically relevant unbound counterparts from the published literature, followed by an assessment of the effect of different molecular descriptors on these PK properties and on the PK-AS slopes for CLtot and Vdss from two species (rat and dog). Lipophilicity (log (D)7.4) and molecular weight (MW) were found to be the most statistically significant and biologically …

In-Vitro Metabolism And Protein Binding Of 5-Hmf, A Potential Antisickling Agent, Taghrid Obied

Theses and Dissertations

Purpose. 5-HMF is a potential antisickling agent forming a Schiff-adduct with hemoglobin (Hb). In-vitro studies were designed to identify the metabolic pathways of 5-HMF in human hepatic cytosol, to assess inter-species differences in its hepatic metabolism, and to predict in-vivo PK properties. Moreover, metabolism of 5-HMF in human RBCs was investigated. Finally, in-vitro studies were done to characterize 5-HMF binding kinetics with human Hb and albumin (HSA). Methods. NAD+ reduction was monitored at 340 nm in human hepatic cytosol for 5-HMF (26 mM) and prototypical ADH and ALDH substrates in the presence or absence of their inhibitors. Furthermore, concentration-dependency studies …

The Role Of The Methyl Dna Binding Domain Protein 2 (Mbd2) In Breast Cancer, Omar Mian

Theses and Dissertations

Methyl-CpG Binding Proteins (MCBPs) are thought to function as the interpreters of epigenetic information encoded in cytosine methylation. Their ability to translate DNA methylation into local transcriptional repression has sparked interest in the role of Methyl-Binding Domain Proteins (MBDs) in cancer, where repatterning of CpG methylation is common. In this dissertation I summarize and discuss observations made in the Ginder Lab linking MCBPs to the progression of neoplastic disease. It is clear from our work that the Methyl Binding Domain Protein 2 (MBD2) is necessary for the persistent repression of critical tumor suppressor genes in breast cancer. We show that …

Dendritic Spine Density Varies Between Unisensory And Multisensory Cortical Regions, Moazzum Bajwa

Theses and Dissertations

In the brain, the dendritic spine is a point of information exchange that extends the neuronal surface on which synapses occur, as well as facilitates and stabilizes those contacts. Furthermore, dendritic spines dynamically change in shape and number in response to a variety of factors. Dendritic spine numbers are reduced in mental retardation, enhanced during development, sensory enrichment or physical exercise, or fluctuate during the reproductive cycle. Thus, for a given neuron type, it might be expected that dendritic spine number might achieve a dynamic optimum. Indeed, many studies of spine density of pyramidal neurons in sensory cortex indicate that …

Ipla2Β, Alternative Splicing And Apoptosis Of Pancreatic Islets, Bhargavi Emani

Theses and Dissertations

Ceramides are bioactive lipids that can promote splicing of apoptosis-related genes, including caspase 9 and BCL-x. A recent study demonstrated that expression of neutral sphingomyelinase (NSMase), an enzyme that hydrolyzes sphingomyelins to generate ceramide, is regulated by Group VIA phospholipase A2 (iPLA2β)-dependent mechanism during β-cell apoptosis. This prompted us to hypothesize that iPLA2 is upstream of ceramide generation in the process regulating splicing of apoptotic genes. To test this, Jurkat T cells were treated with the selective inhibitor of iPLA2β, bromoenol lactone (BEL), RNA was isolated and converted to cDNA, and caspase 9 and BCL-x mRNA viii species were amplified …

Influence Of Retinal States On The Development And Maintenance Of Retinofugal Projections, Duncan Morhardt

Theses and Dissertations

Vision provides a critical interface with the physical world. This work examines visual development and vision loss in mice to glean the influence of the retinal state on visual connections. I first assessed the impact of retinal activity on the eye-specific segregation of retinal afferents in the lateral geniculate nucleus (LGN) of young Gβ5 -/- mice. Gβ5 is the fifth member of the β subfamily of heterotrimeric G proteins. Gβ5 binds and stabilizes the R7 family of regulators of G-protein signaling (RGS), which accelerate Gi/o GTP hydrolysis. Gβ5 -/- mice, which lack R7RGS activity, have malformed synapses in the outer …

Novel Cinnamic Acid-Based Dehydropolymers For Emphysema: In Vitro And In Vivo Assessment Of Their Activities, Bhawana Saluja

Theses and Dissertations

Pulmonary emphysema is a serious worldwide illness, causing progressive and irreversible alveolar wall loss and difficulty in breathing. It is caused mostly by cigarette smoking. However, its unresolved complex and multiple pathogenic mechanisms have left this disease without effective pharmacotherapy. This project hypothesized that cinnamic acid-based dehydropolymers (DHPs), originally discovered as novel anti-coagulants, protect against emphysema through their potent triple inhibitory actions against oxidative stress, inflammation and elastase, some of the pathogenic mechanisms associated with this disease. Three in vitro inhibitory activity assays for oxidative stress, lung inflammation and neutrophil elastase (NE) were developed and used to identify the most …

The Role Of Synaptically Evoked Plateau Potentials In Retinogeniculate Development, Emily Dilger

Theses and Dissertations

We study the activity-dependent refinement of sensory systems by using the mouse retinogeniculate system as a model. Spontaneous retinal waves lead to robust excitatory post-synaptic activity in developing relay cells in the dorsal lateral geniculate nucleus (dLGN) of the thalamus and are reportedly needed to help guide the segregation of retinal inputs into eye-specific domains as well as for the pruning of extraneous retinal inputs onto single dLGN relay cells. The composition of retinally evoked post-synaptic activity activated by these retinal waves in dLGN is largely unknown, but based on our in vitro recordings, such activity seems well suited to …

Patient Ventilator Dyssynchrony: Types, Frequency And Patterns In Critically Ill Adults, Karen Mellott

Theses and Dissertations

Patient ventilator dyssynchrony (PVD) occurs frequently, but little is known about the types, frequency and patterns of PVD for longer than 30 minutes. Deeper levels of sedation are associated with PVD. Evaluation of ventilator graphics and the ability to identify PVD should assist clinicians to optimize patient ventilator interactions and promote earlier interventions. The purpose of this study was to identify the different types, frequency and patterns of PVD in critically ill adults and determine the effect of sedation level on PVD. Thirty medical and surgical ICU adult patients were enrolled; 27 were used for analysis. Pressure/time and flow/ time …

Altered Interactions, Kristen Rego

Theses and Dissertations

Surrounding materials, signage, and detritus on the daily path offer plenty to look at, if not too much. The eye seeks comfort in its passive vision by ignoring its peripheries. Identification of my personal vision reveals itself through the manipulation of ignored material. I consider hand-made vs. machine made, singular vs. the multiple particularly in plastics, packaging and other utilitarian objects. Industrial processes influence my one-person operation. Understanding the way objects are made allows for an opportunity to connect with them. I’m already surrounded by them, the least I can do is get to know them better.

Dreamfall: The Fleeting-Ness Of Memory, Hon Chen

Theses and Dissertations

My work seeks to simulate the impermanence of memory, through the creation of structures and images that translate the mind’s formless but living past into physical material and sensation. The need to search for the missing six years of my childhood memories in Thailand has been the driving force behind the works, along with the lingering emotions of emptiness and unfulfillment. I create multimedia installations with materials, such as plaster, pvc panels, acrylic, polycrylic and dura-lar, to structurally realize a subject as intangible and elusive as memory. Issues of duality, identity, impermanence and memory are underlying themes for my thesis …

Journeys Into The Unknown: A Series Of Science Architecture Tasks And Events, Space-Bound Explorations And Far-Travels, Discoveries And Misses (Near And Far), Imaginative Space-Gazing And Related Investigations, Observations, Orbits, And Other Repetitious Monitoring Tasks, Leah Beeferman

Theses and Dissertations

This thesis expansively and inclusively puts forth the imaginings, research, processes and experiences behind my two thesis exhibitions, "Journeys into the unknown: a series of science architecture tasks and events, space-bound explorations and far-travels, discoveries and misses (near and far), imaginative space-gazing and related investigations, observations, orbits, and other repetitious monitoring tasks" and "Timed travel: asystematic accounts of regular and geometrical timekeeping, orbital flight, repetitive rotations and other journeys into actual time and slow space." It begins with an abstract interpretation of the dial: a tool not limited to scientific measurement but, instead, a gauge of an object’s overall position …

The Everyday In America, Janet Bruhn

Theses and Dissertations

Abstract: My vanilla, Grade A, white bread, run-of-the-mill, middle-of-the-road, threadbare, well-worn, moth-eaten, potato sack, butterscotch, grass stained America: Mundane American life is an existence clinging to the ordinary, where a quilt of mass- mediated preferences and ingrained traditions define many people, specifically from north to south and east to west. Yet, the tastes and dialects of people within the mundane are complex. Ideological preferences are rooted in immigrant history and political persuasion. Various modes of realism have been used by American painters such as The Ash Can School, Regionalists of the 1930’s, and Pop Art. The notion of the real …