Open Access. Powered by Scholars. Published by Universities.®

Digital Commons Network™

Open Access. Powered by Scholars. Published by Universities.®

Virginia Commonwealth University

Discipline
Keyword
Publication Year
Publication
Publication Type
File Type

Articles 2371 - 2400 of 5390

Full-Text Articles in Entire DC Network

Capture And Characterization Of Dioxygen Reactive Intermediates In Cyp51 Catalysis, Gareth Kent Jennings Oct 2012

Capture And Characterization Of Dioxygen Reactive Intermediates In Cyp51 Catalysis, Gareth Kent Jennings

Theses and Dissertations

The cytochromes P450 (CYPs) are a superfamily of biological catalysts that are ubiquitous throughout the biological domain. CYPs are heme-b containing monooxygenases which oxidize substrates with the help of accessory redox partners. CYP substrates include endogenous compounds required for many biological functions and homeostasis, such as steroids, as well as the majority of clinically used drugs and environmental xenobiotics. The majority of studies that have been performed to date are on P450cam (CYP101) from Pseudomonas putida. Of the numerous reactions catalyzed by CYPs, unactivated carbon-carbon bond cleavage, is one of particular versatility. Being unique in their catalytic mechanisms, the C-C …

Go to article

Eastern Shore Stories: Technology, Place, And Local Culture, Patricia Bloxom Oct 2012

Eastern Shore Stories: Technology, Place, And Local Culture, Patricia Bloxom

Theses and Dissertations

The Eastern Shore of Virginia is a narrow peninsula separating the Chesapeake Bay from the Atlantic Ocean. Residents of the rural counties of Accomack and Northampton County share a strong sense of cultural identity based on geography, rooted in a distinct communal sense of place reinforced by an agricultural lifestyle. Storytelling around dinner tables and on front porches at dusk, speeches at high school graduations, family recipes talked through in a grandmother’s kitchen – it is through oral language that Eastern Shore people have primarily shared the knowledge that sustains their sense of communal identity. Oral knowledge of farming techniques …

Go to article

Commonwealth Times 2012-10-11 Oct 2012

Commonwealth Times 2012-10-11

Commonwealth Times, 1969-

No abstract provided.

Go to article

The Unclothed Body In Francisco Goya's The Disasters Of War, Elizabeth Sanderford Oct 2012

The Unclothed Body In Francisco Goya's The Disasters Of War, Elizabeth Sanderford

Theses and Dissertations

Francisco Goya (1746-1828) created a series of prints entitled The Disasters of War (1810-1820) (Los Desastres de la Guerra) depicting the Napoleonic Wars in Spain, the famine that subsequently occurred in Madrid, and symbolic representations similar to his print series Los Caprichos (1797-1799). This thesis will consider Goya’s use of the unclothed human body in his series of prints The Disasters of War. This thesis will place these representations within the contexts of other works produced by Goya that portray the unclothed figures, as well as within the Spanish artistic tradition of Goya’s time. Through this investigation, I will situate …

Go to article

Commonwealth Times 2012-10-08 Oct 2012

Commonwealth Times 2012-10-08

Commonwealth Times, 1969-

No abstract provided.

Go to article

On The Genetic And Environmental Associations Between Body Composition, Depression Symptoms And Smoking Behavior., Roseann Peterson Oct 2012

On The Genetic And Environmental Associations Between Body Composition, Depression Symptoms And Smoking Behavior., Roseann Peterson

Theses and Dissertations

Obesity is a serious public health crisis and recent estimates of its incidence are the highest in United States history, with 35% and 17% of American adults and children affected, respectively. The clinical definition of adult obesity is operationalized as a body mass index (BMI) greater than 30 kg/m2. Although the prevalence of common obesity has increased dramatically over the past 30 years–largely thought to be due to changes in the environment, such as high calorie diets and sedentary lifestyles—twin and family studies have shown consistently that relative body weight is under considerable genetic influence in both children and adults, …

Go to article

Commonwealth Times 2012-10-04 Oct 2012

Commonwealth Times 2012-10-04

Commonwealth Times, 1969-

No abstract provided.

Go to article

Examining Alcohol Dependence And Its Correlates From A Genetically Informative Perspective, Laura Hack Sep 2012

Examining Alcohol Dependence And Its Correlates From A Genetically Informative Perspective, Laura Hack

Theses and Dissertations

Alcohol dependence (AD) is a serious and common public health problem that contributes to great societal, medical, and legal costs. It has taken work from multiple disciplines, including developmental psychology, genetic epidemiology, and molecular genetics, to achieve our current understanding of environmental and genetic risk factors for AD as well as its variable developmental trajectories. Nevertheless, there is still much to be learned in order to improve treatment outcomes. One approach to augmenting our understanding of this disorder is through genetically informative study designs that either examine risk in aggregate or assess specific susceptibility variants. In this dissertation, we utilize …

Go to article

Using Genetic Information In Risk Prediction For Alcohol Dependence, Jia Yan Sep 2012

Using Genetic Information In Risk Prediction For Alcohol Dependence, Jia Yan

Theses and Dissertations

Family-based and genome-wide association studies (GWAS) of alcohol dependence (AD) have reported numerous associated variants. The clinical validity of these variants for predicting AD compared to family history has not yet been reported. These studies aim to explore the aggregate impact of multiple genetic variants with small effect sizes on risk prediction in order to provide a clinical interpretation of genetic contributions to AD. Data simulations showed that given AD’s prevalence and heritability, a risk prediction model incorporating all genetic contributions would have an area under the receiver operating characteristic curve (AUC) approaching 0.80, which is often a target AUC …

Go to article

Role Of Autophagy In The Response Of Hs578t Breast Tumor Cells To Radiation, Shweta Chakradeo Sep 2012

Role Of Autophagy In The Response Of Hs578t Breast Tumor Cells To Radiation, Shweta Chakradeo

Theses and Dissertations

Breast cancer is the most commonly observed cancer type in women and is the second leading cause of cancer death in women. Radiation can be used to debulk tumors prior to surgery as well as to treat patients after surgery and/or chemotherapy. Previous studies from our laboratory have shown that the anti –malarial drug chloroquine sensitizes breast cancer cell lines to radiation by suppression of autophagy which is a conservative catabolic process that can be cytoprotective. The scientific literature has demonstrated that many tumor cell systems undergo cytoprotective autophagy and that pharmacological or genetic inhibition of autophagy leads to other …

Go to article

Using Macroinvertebrate Community Composition To Distinguish Between Natural And Anthropogenic Sedimentation, Amanda E. Schutt Sep 2012

Using Macroinvertebrate Community Composition To Distinguish Between Natural And Anthropogenic Sedimentation, Amanda E. Schutt

Theses and Dissertations

Excess fine sediment from human activity is a major pollutant to streams across the U.S.; however, distinguishing human-induced sedimentation from natural fine sediment is complex. The U.S. Environmental Protection Agency recently implemented a protocol for the quantitative field assessment of human-induced sedimentation using measurements of stream geomorphology. Macroinvertebrate community composition, streambed sediment stability, and sediment composition were studied at 49 sites in the James River watershed in central Virginia. Sediment composition was found to be a stronger driver of community composition than sediment stability. Although I was not able to show that macroinvertebrate metrics were related to sediment stability independently …

Go to article

Commonwealth Times 2012-09-06 Sep 2012

Commonwealth Times 2012-09-06

Commonwealth Times, 1969-

No abstract provided.

Go to article

Ink (2012-09) Sep 2012

Ink (2012-09)

Ink, 2008-

The student-run features magazine covering the fashion, art, music and culture worlds at VCU and in Richmond. Published annually in print and updated regularly online.

Go to article

Role Of Nicotinic Acetylcholine Receptors In Experimental Colitis, Shakir Alsharari Aug 2012

Role Of Nicotinic Acetylcholine Receptors In Experimental Colitis, Shakir Alsharari

Theses and Dissertations

Substantial evidence in the literature shows that tobacco smoking has complex and divergent effects on inflammatory bowel diseases (IBD). It ameliorates ulcerative colitis (UC); whereas it aggravates the risk of Crohn’s disease (CD) and affects the disease course and severity. Studies have shown that nicotine has a positive influence on symptoms of UC. Also, it is demonstrated that nicotinic acetylcholine receptor, especially α7 subunit plays an essential component in the vagus nerve-based cholinergic anti-inflammatory effects. In the present study, we explored the effect of nicotine and α7 nicotinic agonists treatment in the DSS colitis mouse model. We also investigated the …

Go to article

Influence Of Electrostatic Charge Upon The Deposition Behavior Of Pharmaceutical Aerosols Within Cascade Impactors, Megha Mohan Aug 2012

Influence Of Electrostatic Charge Upon The Deposition Behavior Of Pharmaceutical Aerosols Within Cascade Impactors, Megha Mohan

Theses and Dissertations

Cascade impactors, routinely used for in vitro particle size characterization of pharmaceutical aerosols, are calibrated using dilute, charge-neutralized, monodisperse aerosols. But pharmaceutical aerosols are known to generate concentrated, inherently charged, polydisperse aerosol clouds. A computational model of the Andersen Cascade Impactor (ACI) suggested that the presence of charge on aerosol particles may influence their deposition within the ACI, but experimental validation of the model is warranted. This dissertation investigates the influence of electrostatic charge upon the deposition behavior of aerosols within cascade impactors, to address the impact of charge on particle size characterization. The influence of applied charge upon the …

Go to article

Α2-Adrenoceptor And 5-Ht3 Serotonin Receptor Ligands As Potential Analgesic Adjuvants, Genevieve Alley Aug 2012

Α2-Adrenoceptor And 5-Ht3 Serotonin Receptor Ligands As Potential Analgesic Adjuvants, Genevieve Alley

Theses and Dissertations

There continues to be a need for more effective analgesics. The α2-adrenoceptor (AR) agonist clonidine is an analgesic whose use is severely limited by undesirable side effects. meta-Chlorophenylguanidine (MD-354), an agent developed in our laboratory, selectively potentiates the antinociceptive effects of clonidine in a biphasic manner. Mechanistic studies suggest that both 5-HT3 receptor and α2-AR mechanisms are involved. To further evaluate mechanisms underlying the analgesia-potentiating effect of clonidine by MD-354, pharmacological studies using more established 5-HT3 receptor agonists: meta-chlorophenylbiguanide (mCPBG) and centrally-acting SR57227A, and non-selective α2-adrenoceptor ligand TDIQ, administered alone and in combination with clonidine, were conducted in mouse antinociceptive …

Go to article

Investigating Synergy Between Ribonucleotide Reductase Inhibitors And Cmv Antivirals, Sukhada Bhave Aug 2012

Investigating Synergy Between Ribonucleotide Reductase Inhibitors And Cmv Antivirals, Sukhada Bhave

Theses and Dissertations

Cytomegalovirus (CMV) infections remain a significant problem in congenitally infected infants and immunocompromised individuals. Modest antiviral activities of currently approved drugs coupled with dose-limiting toxicities restrict effectiveness and promote development of resistance. The potential for ribonucleotide reductase (RR) inhibitors hydroxyurea (HU), Didox, and Trimidox to synergize, through reduction of nucleotide pools, with the deoxynucleotide analog Ganciclovir (GCV) was examined. A yield reduction assay that utilizes luciferase expressed by a recombinant virus as a surrogate measure of viral infectious units was developed and used to determine effective dose ranges for each drug. RR inhibitors exhibited intrinsic anti-CMV activities on their own …

Go to article

A Study Towards Development Of An Automated Haptic User Interface (Ahui) For Individuals Who Are Blind Or Visually Impaired, Ravi Rastogi Aug 2012

A Study Towards Development Of An Automated Haptic User Interface (Ahui) For Individuals Who Are Blind Or Visually Impaired, Ravi Rastogi

Theses and Dissertations

An increasing amount of information content used in schools, work and everyday living is being presented in graphical form, creating accessibility challenges for individuals who are blind or visually impaired, especially in dynamic environments, such as over the internet. Refreshable haptic displays that can interact with computers can be used to access such information tactually. Main focus of this study was the development of specialized computer applications allowing users to actively compensate for the inherent issues of haptics when exploring visual diagrams as compared to vision, which we hypothesized, would improve the usability of such devices. An intuitive zooming algorithm …

Go to article

Mass Spectroscopic Identification And Quantification Of Protein Carbonyls, Zafer Ugur Aug 2012

Mass Spectroscopic Identification And Quantification Of Protein Carbonyls, Zafer Ugur

Theses and Dissertations

It is well established that free radical mediated oxidative stress plays a critical role in aging and age-related diseases. Among the post-translational protein modifications, carbonylation has attracted a great deal of attention due to its irreversible and irreparable nature. Despite the fact that protein carbonylation is associated with a series of physiological and pathological processes, there are still issues to be clarified such as why certain proteins are more vulnerable to modification, what are the locations of the protein modifications, and how does the nature of the oxidant affect the preferred site of modification. In this study, we will seek …

Go to article

Purification And Reconstitution Into Planar Bilayers Of The Human Dopamine Transporter, Yoori Kim Aug 2012

Purification And Reconstitution Into Planar Bilayers Of The Human Dopamine Transporter, Yoori Kim

Theses and Dissertations

The human dopamine transporter (hDAT) provides the primary mechanism for dopamine clearance in synapses and thus facilitates the regulation of dopaminergic functions in cognition and reward. It is the molecular target of many centrally-active agents including amphetamines and cocaine. Therefore, an understanding of hDAT function and its modulation by these therapeutic drugs and drugs of abuse can provide insight into the mechanisms of abuse and addiction. In the presented studies, hDAT is tagged with a hexahistidine construct and heterologously expressed in Xenopus laevis oocytes. The plasma membranes are isolated, solubilized, and applied to a Nickel affinity column to obtain purified …

Go to article

Investigating The Transcriptional Regulation By Oxyr In Porphyromonas Gingivalis., Anuya R. Paranjape Aug 2012

Investigating The Transcriptional Regulation By Oxyr In Porphyromonas Gingivalis., Anuya R. Paranjape

Theses and Dissertations

Periodontal diseases are bacterially induced, inflammatory diseases which are responsible for loss of alveolar bone and connective tissue supporting the teeth which results in loss of teeth. Gram negative anaerobic bacteria are highly associated with these diseases. One of them is Porphyromonas gingivalis belonging to the phylum Bacteroidetes. Infection by P. gingivalis is recurrent after physical removal of the bacteria from the oral cavity and even after antibiotic treatment as development of resistance is not rare. Hence complete understanding the biology of this bacterium is of significance. This gram negative obligate anaerobe, being aerotolerant, manages to survive inside the oral …

Go to article

Modeling The Resistance To Hydrostatic Pressures For Superhydrophobic Coatings With Random Roughness, Thomas Michael Bucher Jr. Aug 2012

Modeling The Resistance To Hydrostatic Pressures For Superhydrophobic Coatings With Random Roughness, Thomas Michael Bucher Jr.

Theses and Dissertations

A superhydrophobic coating can be produced using a hydrophobic material textured with surface roughness on the micro-/nano-scale. Such a coating on the outside of a submersible body may result in reduced skin-friction drag due to a trapped layer of air in the coating. However, this layer may become unstable when subjected to elevated hydrostatic pressures, and a coating’s performance is compromised beyond a certain threshold (critical pressure). This thesis presents a numerical model for predicting the pressure tolerances of superhydrophobic coatings comprised of randomly deposited hydrophobic particles or fibers. We have also derived a set of force-balance-based analytical equations for …

Go to article

Essential Spawning Habitat For Atlantic Sturgeon In The James River, Virginia., Geoffrey Austin Aug 2012

Essential Spawning Habitat For Atlantic Sturgeon In The James River, Virginia., Geoffrey Austin

Theses and Dissertations

Substrate composition plays a critical role in determining the spawning success of Atlantic sturgeon. A benthic analysis of the tidal freshwater portion of the James River, Virginia, was performed to locate and protect remaining sturgeon spawning habitat within the James River system. I modeled structural habitat, substrate distribution, and river bathymetry from Richmond, Virginia to the Appomattox River confluence. A classification model was developed to describe the dominant substrate type (mud/silt, sand, gravel, bedrock) using side scan sonar data collected from August 2011-Febuary 2012. River depth, bottom imagery, substrate density (hardness), and ground truth substrate samples were interpolated into a …

Go to article

The Severity Of Stages Estimation During Hemorrhage Using Error Correcting Output Codes Method, Yurong Luo Aug 2012

The Severity Of Stages Estimation During Hemorrhage Using Error Correcting Output Codes Method, Yurong Luo

Theses and Dissertations

As a beneficial component with critical impact, computer-aided decision making systems have infiltrated many fields, such as economics, medicine, architecture and agriculture. The latent capabilities for facilitating human work propel high-speed development of such systems. Effective decisions provided by such systems greatly reduce the expense of labor, energy, budget, etc. The computer-aided decision making system for traumatic injuries is one type of such systems that supplies suggestive opinions when dealing with the injuries resulted from accidents, battle, or illness. The functions may involve judging the type of illness, allocating the wounded according to battle injuries, deciding the severity of symptoms …

Go to article

I Am Error, Nathan Altice Aug 2012

I Am Error, Nathan Altice

Theses and Dissertations

I Am Error is a platform study of the Nintendo Family Computer (or Famicom), a videogame console first released in Japan in July 1983 and later exported to the rest of the world as the Nintendo Entertainment System (or NES). The book investigates the underlying computational architecture of the console and its effects on the creative works (e.g. videogames) produced for the platform. I Am Error advances the concept of platform as a shifting configuration of hardware and software that extends even beyond its ‘native’ material construction. The book provides a deep technical understanding of how the platform was programmed …

Go to article

Spag16 Is A Bifunctional Gene Regulating Male Fertility, David R. Nagarkatti-Gude Jul 2012

Spag16 Is A Bifunctional Gene Regulating Male Fertility, David R. Nagarkatti-Gude

Theses and Dissertations

SPAG16 is the murine orthologue of Chlamydomonas reinhardtii PF20, a protein known to be essential to the structure and function of the “9 + 2” axoneme. The “9 + 2” axoneme provides the cytoskeletal core of all eukaryotic motile cilia and flagella. In Chlamydomonas, the Pf20 gene encodes a single protein present in the central pair of the axoneme. Loss of Pf20 prevents central pair assembly and results in flagellar paralysis. The murine Spag16 gene encodes two proteins. While 71 kDa SPAG16L is found in all murine cells with motile cilia or flagella, 35 kDa SPAG16S transcript and protein are …

Go to article

Effects Of Leaf Litter Diversity On Nutrients And Mosquito Communities In Neotropical Artificial Tree Holes, Rachel Komosinski Jul 2012

Effects Of Leaf Litter Diversity On Nutrients And Mosquito Communities In Neotropical Artificial Tree Holes, Rachel Komosinski

Theses and Dissertations

Inputs from terrestrial habitats to aquatic habitats are important for structuring aquatic communities. Terrestrial producer diversity in the tropics may decline due to anthropogenic causes. I investigated how tree diversity affects aquatic communities. We used leaves from three timber-producing species (Dalbergia retusa, Pachira quinata, and Tectona grandis) to test the effects of leaf litter species composition and richness on invertebrate aquatic communities in Gamboa, Panama. We quantified macroinvertbrate species richness and abundances, leaf litter mass loss, and dissolved carbon to nitrogen ratios (C:N) after 4 weeks. We found that litter types differed in breakdown and C:N. Tectona grandis had lower …

Go to article

The “Man Walks Outside Time Now”: Verbal Representations Of Photographic Images In The Poems Of Larry Levis, Lauren Miner Jul 2012

The “Man Walks Outside Time Now”: Verbal Representations Of Photographic Images In The Poems Of Larry Levis, Lauren Miner

Theses and Dissertations

The poet Larry Levis often employed ekphrasis as an elegiac device—particularly with his verbal descriptions of photographic images—to explore human suffering and reconcile feelings of loss. Through the ekphrastic mode, Levis could juxtapose otherwise disparate images, manipulating their temporal and spatial relationships, to achieve what he conceived an authentic portrait of the human experience. The poet, through his verbal descriptions of photographic images, does not try to evade the pain or joy of being human; instead, he confronts his grief directly and, in so doing, transcends that suffering to better understand himself and his own human position. This thesis analyzes …

Go to article

The Effect Of A Navigational Aid On Training Of A Minimally Invasive Surgery Camera Task, Ketan Vidwans Jul 2012

The Effect Of A Navigational Aid On Training Of A Minimally Invasive Surgery Camera Task, Ketan Vidwans

Theses and Dissertations

Minimally Invasive Surgery (MIS) differs from Open Surgery as surgeons view the surgical site indirectly on a monitor. The view shown is typically from an angled endoscope off to one side of the surgery (i.e., uncollocated with the view of the hands). This makes camera navigation a challenging ability to learn. MIS thus requires longer training periods, more practice and mental effort to achieve proficiency. Current training setups and Operating Room (OR) environments lack appropriate real-time visual cues for navigation and other perception related information that could help with learning and performance in the OR. The purpose of this research …

Go to article

Establishing The Discriminative Stimulus Properties Of The Atypical Antipsychotic Amisulpride In C57bl/6 Mice, Timothy J. Donahue Jul 2012

Establishing The Discriminative Stimulus Properties Of The Atypical Antipsychotic Amisulpride In C57bl/6 Mice, Timothy J. Donahue

Theses and Dissertations

Abstract ESTABLISHING THE DISCRIMINATIVE STIMULUS PROPERTIES OF THE ATYPICAL ANTIPSYCHOTIC AMISULPRIDE IN C57BL/6 MICE Antipsychotic medications are used to treat schizophrenia. The present study used the drug discrimination paradigm to measure the subjective effects of the atypical antipsychotic amisulpride and to examine the underlying neuropharmacological mechanisms responsible for the discriminative stimulus property of the drug. Male C57BL/6 mice were trained to discriminate 10 mg/kg (-)S amisulpride from vehicle in a two-lever drug discrimination task. A dose effect curve for (-)S amisulpride yielded an ED50 = 1.77 mg/kg 95% CI [1.28, 2.45 mg/kg]. Substitution testing was conducted for the isomer (+)R …

Go to article

First Prev Next Last