Digital Commons Network^™

Reproductive Success In Hatchery-Produced Eastern Oyster, Crassostrea Virginica (Gmelin), April Piggott

Theses and Dissertations

The eastern oyster (Crassostrea virginica Gmelin 1791) has great ecological and economic importance but populations have declined, especially in Chesapeake Bay, to historically low numbers. Hatcheries strive to produce oysters with beneficial characteristics for supplementation and commercial purposes, both natural and stimulated mass spawning. Unequal contribution of parents in mass spawnings potentially can lead to high levels of inbreeding and a loss of beneficial characteristics in offspring. In this study, we determined microsatellite genotypes for parents (n^parents =24, 49, and 77 parents) and progeny (n=96 each) of three hatchery-produced families and used the data for parental assignment. We observed the …

Capillarity And Two-Phase Fluid Transport In Media With Fibers Of Dissimilar Properties, Thomas M. Bucher Jr.

Theses and Dissertations

Capillarity is a physical phenomenon that acts as a driving force in the displacement of one fluid by another within a porous medium. This mechanism operates on the micro and nanoscale, and is responsible for countless observable events. This can include applications such as absorption in various hygiene products, self-cleaning surfaces such as water beading up and rolling off a specially-coated windshield, anti-icing, and water management in fuel cells, among many others.

The most significant research into capillarity has occurred within the last century or so. Traditional formulations for fluid absorption include the Lucas–Washburn model for porous media, which is …

Story-Telling Through The Design Of A Permanent Mission Trip Training And Housing Facility, Betty L. Rowe

Theses and Dissertations

This mixed- assembly space is designed as a permanent mission trip training and housing facility. Predominately the space will be used by middle school and high school youth. By creating a modern experience, teens are given a better opportunity to engage in a space they can relate to. It becomes a space where young people feel encouraged to share their feelings, beliefs and desires as they journey into a closer, more intimate relationship with a higher being.

The space is intended to serve a transient population. A large portion of the first floor is devoted to communal gathering which provides …

Rhetorical Ripples: The Church Of The Subgenius, Kenneth Burke & Comic, Symbolic Tinkering, Lee A. Carleton

Theses and Dissertations

Humor has long been an effective way to engage difficult sociopolitical topics in a way that avoids polemical confrontation and provides opportunity for pleasure, catharsis and self-knowledge. In the context of today’s polarized politics and protest, creative satirical performance that deploys “symbolic tinkering” can provide a “comic frame of reference” that, according to Kenneth Burke, more effectively conveys its message while providing reflexive insight. The satirical Church of the SubGenius naturally practices this rhetorical frame in their multimedia creations. Using the lens of Burke’s Attitudes Toward History, this essay is an analysis of SubGenius rhetoric with a focus on …

The E-Volving Picturebook: Examining The Impact Of New E-Media/Technologies On Its Form, Content And Function (And On The Child Reader), Stella K. Reinhard

Theses and Dissertations

The technology of the codex book and the habit of reading appear to be under attack currently for a variety of reasons explored in the Introduction of this Dissertation. One natural response to attack is a resulting effort to adapt in a bid to survive. Noël Carroll, leading American philosopher in the contemporary philosophy of art, touches on this concept in his discussion of the evolution of a new medium in his article, “Medium Specificity Arguments and Self-Consciously Invented Arts: Film, Video, and Photography,” from his Cambridge University Press 1996 text, Theorizing the Moving Image. Carroll proposes that any …

Novel Therapeutic Strategies For Pancreatic Cancer, Bridget A. Quinn

Theses and Dissertations

Pancreatic cancer is a devastating disease that leaves patients with a very poor prognosis and few therapeutic options. Many of the treatment options available are the same that have been used for almost 2 decades. There is a dire need for both novel treatments for this disease as well as novel strategies of treatment. This body of work will introduce and provide evidence in support of a novel combination therapy for pancreatic cancer treatment, a novel strategy of modifying currently used chemotherapeutics for pancreatic cancer therapy, and a novel transgenic preclinical mouse model of pancreatic cancer. Sabutoclax, an antagonist of …

The Effect Of Obesity On Il-1Β, Il-1ra, And Leptin Following Acute Mental Stress, Heather Caslin

Theses and Dissertations

Research regarding the development of cardiovascular disease (CVD) is important because CVD is the leading cause of death in the United States (US) and many countries abroad. Risk factors, such as obesity and psychological stress, should be studied in order to understand contributing factors for CVD and the cellular mechanisms which link risk factors with the development of disease. Specifically, the combined influence of multiple risk factors on inflammation is of interest because many individuals have more than one risk factor, which additively increases an individual’s risk for CVD. Obesity is already characterized by disordered inflammation, which suggests that the …

Regulation Of Ykl-40 In Sterile Inflammation And Its Role In Glioblastoma In Vivo, Reetika Bhardwaj

Theses and Dissertations

YKL-40 is a secreted glycoprotein, which is a shared biomarker of chronic inflammation and oncogenic transformation. Indeed, YKL-40 expression is up-regulated in many diseases including multiple sclerosis, Alzheimer disease, viral encephalitis, HIV-associated dementia, brain infarction, and traumatic brain injury. YKL-40 is also expressed by several solid tumors, such as osteosarcoma, ovarian carcinoma and glioblastoma multiforme (GBM). It promotes the migration and invasion of astrocytes as well as GBM cells. Serum YKL-40 levels have been shown to directly correlate with tumor grade and potentially tumor burden in GBM. In contrast to the numerous reports documenting elevated expression of YKL-40, relatively little …

Developing A Caenorhabditis Elegans Model For Marfan Syndrome, Pauline Fotopoulos

Theses and Dissertations

Marfan Syndrome (MFS) is one of the most common monogenic diseases and affects approximately 1 in 5,000 individuals worldwide. The syndrome is characterized by elongated extremities, tall stature, slender frame, and cardiac, and vision abnormalities due to severe connective tissue defects. It is caused by mutations in the fbn1 gene, which encodes an extracellular matrix glycoprotein, and is required for proper cardiac and skeletal development and for sequestration of TGFβ (transforming growth factor beta) and BMP (bone morphogenetic protein) within the extracellular matrix (ECM). The primary objective of this study was to establish a C.elegans MFS model and use this …

Vintage Anxiety, Bradford Pearce

Theses and Dissertations

Poems

Differential Effects Of Endocannabinoid Catabolic Inhibitors On Opioid Withdrawal In Mice, Thomas Gamage

Theses and Dissertations

The effects of cannabinoids in reducing somatic signs of opioid withdrawal have been known for some time. In morphine dependent rodents, opioid withdrawal following precipitation with the mu opioid antagonist naloxone elicits robust withdrawal behaviors including jumps, paw flutters, head shakes, diarrhea and weight loss. Delta-9-tetrahydrocannabinol has been shown to reduce this opioid withdrawal in mice via activation of the cannabinoid type-1 (CB1) receptor and recently it has been shown that inhibition of the catabolic enzymes for endocannabinoids also reduce somatic signs of opioid withdrawal. Specifically, inhibition the enzyme fatty acid amide hydrolase (FAAH), the catabolic enzyme for the endocannabinoid …

Environmental Regulation Of Tidal Wetland Microbial Communities And Associated Biogeochemistry, Ember Morrissey

Theses and Dissertations

Microbial communities play an essential role in carrying out the biogeochemical cycles that sustain life on Earth, yet we know very little about their ecology. One question of particular interest is how environmental conditions shape microbial community structure (i.e., the types of organisms found in the community and their relative abundance), and whether such changes in structure are related to biogeochemical function. It is the aim of this dissertation to address this question via the examination of carbon (C) and nitrogen (N) cycling in wetland ecosystems, which due to their diverse hydrology have a profound influence on biogeochemical cycles. With …

Real Time Monitoring Of Surface Chemical Events By Streaming Potentials In Microfluidic Channels, Kenji Nokura

Theses and Dissertations

Zeta potential is one of the tools to measure the surface charge of materials, and Alvarez et al. have developed a microchannel device to measure zeta potentials in real time for label-free sensing using immobilized receptors on microchannel surfaces. However, the challenge has been the charge interference of surface modifiers on analyte detection. Therefore, it was necessary to find the best strategy to regenerate minimal surface charge after modifying the channel with polymer films that would anchor the affinity groups for the analyte. It was demonstrated that adsorption of positively and negatively charged analytes were monitored via real time zeta …

Materials And Meaning, Michael Corigliano

Theses and Dissertations

Decisive moments and their fleeting experiences are born from an individual’s interaction within a defined physical space. It is here at the intersection of environment and context that my work in site-specific art begins. I endeavor to create an examination of socio-political and environmental issues through a manipulation of materials, thereby altering one’s private, communal, and cultural response to them. My installations are comprised of slip casted multiples which reference the human form. I place these forms in galleries and specific exterior locations, and incorporate materials that are charged with societal discord, such as used motor oil and post-consumer detritus, …

The Response Of Naive Channel Catfish To Chemical Cues Associated With Predation, Marinda Coulter

Theses and Dissertations

The chemosensory abilities of fishes, are important in order to understand how prey can perceive and avoid predators. Predator-naïve Channel catfish were exposed to four extracts over eight sessions (naïve bass water (NBW), bass that were fed catfish water (BFCW), catfish skin extract (CS), and naïve bass water paired with catfish skin extract (CO)) to determine whether they have an innate predator response to potential chemical cues indicating possible predation risk. Movement was quantified as grid squares crossed, directional changes, and tail beats. Response increased during the first minute following stimulus injection and decreased during the second minute. Channel catfish …

Multi-Column Multi-Layer Computational Model Of Neocortex, Beata Strack

Theses and Dissertations

We present a multi-layer multi-column computational model of neocortex that is built based on the activity and connections of known neuronal cell types and includes activity-dependent short term plasticity. This model, a network of spiking neurons, is validated by showing that it exhibits activity close to biology in terms of several characteristics: (1) proper laminar flow of activity; (2) columnar organization with focality of inputs; (3) low-threshold-spiking (LTS) and fast-spiking (FS) neurons function as observed in normal cortical circuits; and (4) different stages of epileptiform activity can be obtained with either increasing the level of inhibitory blockade, or simulation of …

Tobramycin Disposition In The Lung Following Airway Administration, Min Li

Theses and Dissertations

Tobramycin disposition following airway administration was evaluated by meta-analysis of human data in the literature and, experimentally, using a realistic ex vivo model, the isolated perfused rat lung preparation (IPRL). Pulmonary bioavailability of inhaled tobramycin in published studies was re-evaluated separately for CF and healthy adults, with the drug’s intrinsic pharmacokinetic (PK) parameters obtained from intravenous (IV) studies in the literature. While large variations in tobramycin’s clearance precluded accurate assessment of its bioavailability, the results were indicative of substantial pulmonary absorption, in spite of its hydrophilic and poly cationic properties. To explore its disposition kinetics and mechanisms following airway administration, …

A New Beginning, Nancy Rae Habit

Theses and Dissertations

Artist Statement My artwork reflects elements of simple, everyday life. I find poignancy in people as they go about their daily business, children playing on the beach or an elderly woman at her kitchen stove. And, it’s not just people in whom I find intrigue. The creases and folds of crumpled, brown paper bags; fresh fish lying on crushed ice in outdoor markets; and household objects, such as chairs, irons, and wooden spoons are the types of things that are also evidence of daily life. In my work, I strive to replicate with both accuracy and emotion all of these …

Who's Your Daddy? A Study Of Extra-Pair Copulation And Mating Behaviors Of Protonotaria Citrea, Morton Catherine Heidrich

Theses and Dissertations

Mating behavior has a profound impact on reproductive success and the resulting genetic structure of offspring. Extra-pair copulation is a widely observed behavior within avian species. This study explored the genetic effects of mating behaviors of Prothonotary warblers, Protonotaria citrea (Parulidae), using co-dominant microsatellite markers. Prothonotary warblers are migratory songbirds that build nests in cavities, commonly found in wetland habitats. A set of artificial nest boxes were initiated by Dr Robert Reilly in 2002 in Dutch Gap, Chesterfield County Virginia, USA, a tidal tributary off the James River. From this population, 28 nest boxes were surveyed yielding 47 adults and …

Dissecting Emotion, Jennifer Lopresti

Theses and Dissertations

My art making approach has enabled me to focus on the connection between our anatomical makeup and the inner workings of the mind. It has been a journey to personally bridge the gap between the two and to make sense of the overlapping functions. Anatomical imagery has lent itself to my exploration into the topics of transformation and social interaction, and the emotions that are tied to these concepts. My body of work consists of mixed media and sculptural pieces. I make use of found objects such as vinyl records, rotary phones, and old black and white photographs. These items …

Transcriptional Crosstalk Between Helper Bacteriophages And Staphylococcal Aureus Pathogenicity Islands, Kristin Lane

Theses and Dissertations

Acquisition of a superantigen pathogenicity island (SaPI) significantly increases virulence in Staphylococcus aureus. Horizontal transfer of SaPIs occurs at high frequency and depends upon a helper bacteriophage, either through direct infection or SOS-mediated induction of a lysogen. SaPIs hijack the packaging machinery of the helper phage, leading to the formation of SaPI-containing transducing particles that can introduce the pathogenicity island into neighboring SaPI-negative cells. All SaPIs contain a conserved core of genes, some of which are co-transcribed as an operon and encode functions involved in helper exploitation. The goal of this study was to more fully understand the intricate relationships …

Effects Of Thoracic Spinal Manipulative Therapy On Thoracic Spine And Shoulder Kinematics, Thoracic Spine Flexion/Extension Excursion, And Pressure Pain Sensitivity In Patients With Subacromial Pain Syndrome, Joseph Kardouni

Theses and Dissertations

EFFECTS OF THORACIC SPINAL MANIPULATIVE THERAPY ON THORACIC SPINE AND SHOULDER KINEMATICS, THORACIC SPINE FLEXION/EXTENSION EXCURSION, AND PRESSURE PAIN SENSITIVITY IN PATIENTS WITH SUBACROMIAL PAIN SYNDROME By Joseph R. Kardouni, Ph.D., PT A dissertation submitted in partial fulfillment of the requirements for the degree of Doctor of Philosophy, at Virginia Commonwealth University. Virginia Commonwealth University, 2013. Major Director: Lori A. Michener, PhD, PT, ATC, Professor, Department of Physical Therapy In patients with shoulder pain, the use of manual therapy directed at the spine and shoulder have been reported to provide superior outcomes to exercise based interventions or usual care without …

Dead Virginians: The Corpse And Its Uses In Early Virginia, David Roettger

Theses and Dissertations

The thesis traces the history of colonial Virginia in an attempt to uncover the origins of several peculiarities in Virginia death-ways. Elite Virginians buried at home more often than not (where they could protect the dead from animal desecration), while avoided death’s heads, reapers, and bone based tomb and mourning jewelry iconography even though such was popular throughout the British Atlantic. Research done for this thesis reveals a fear on the part of elite Virginias regarding questions of both corpse desecration and natural putrefaction. The cause of this cultural obsession lie in two facts: The blackening of the early colony’s …

Metabolic Remodeling And Mitochondrial Dysfunction In Maladaptive Right Ventricular Hypertrophy Secondary To Pulmonary Arterial Hypertension, Jose Gomez-Arroyo

Theses and Dissertations

Right ventricular dysfunction is the most frequent cause of death in patients with pulmonary arterial hypertension. Although abnormal energy substrate use has been implicated in the development of chronic left heart failure, data describing such metabolic remodeling in failing right ventricular tissue remain incomplete. In the present dissertation we sought to characterize metabolic gene expression changes and mitochondrial dysfunction in functional and dysfunctional RV hypertrophy. Two different rat models of RV hypertrophy were studied. The model of right ventricular failure (SU5416/hypoxia) exhibited a significantly decreased gene expression of peroxisome proliferator-activated receptor- coactivator-1α, peroxisome proliferator- activated receptor-α and estrogen-related receptor-α. The …

On Relay Node Placement Problem For Survivable Wireless Sensor Networks, Changyong Jung

Theses and Dissertations

Wireless sensor networks are widely applied to many fields such as animal habitat monitoring, air traffic control, and health monitoring. One of the current problems with wireless sensor networks is the ability to overcome communication failures due to hardware failure, distributing sensors in an uneven geographic area, or unexpected obstacles between sensors. One common solution to overcome this problem is to place a minimum number of relay nodes among sensors so that the communication among sensors is guaranteed. This is called Relay Node Placement Problem (RNP). This problem has been proved as NP-hard for a simple connected graph. Therefore, many …

Hybrid Pet/Mri Nanoparticle Development And Multi-Modal Imaging, David Hoffman

Theses and Dissertations

The development of hybrid PET/MRI imaging systems needs to be paralleled with the development of a hybrid intrinsic PET/MRI probes. The aim of this work was to develop and validate a novel radio-superparamagnetic nanoparticle (r-SPNP) for hybrid PET/MRI imaging. This was achieved with the synthesis of superparamagnetic iron oxide nanoparticles (SPIONs) that intrinsically incorporated 59Fe and manganese iron oxide nanoparticles (MIONs) that intrinsically incorporated 52Mn. Both [59Fe]-SPIONs and [52Mn]-MIONs were produced through thermal decomposition synthesis. The physiochemical characteristics of the r-SPNPs were assessed with TEM, DLS, and zeta-potential measurements, as well as in imaging phantom studies. The [59Fe]-SPIONs were evaluated …

Reichenbachia, Imperial Edition: Rediscovering Frederick Sander’S Late-Victorian Masterpiece Of Botanical Art, Erica Borey

Theses and Dissertations

This thesis project examines the history, provenance, and contemporary treatment of a rare Imperial Edition of Frederick Sander’s print collection Reichenbachia, Orchids Illustrated and Described, a high-quality orchid compendium dating to the late-nineteenth century. A local philanthropist loaned the Imperial Edition Reichenbachia, number 86 of 100 to Lewis Ginter Botanical Garden in 2011 on a long-term basis as a promised donation. Research into the origins of this collection involves several disparate historical topics, including the Victorian period of “orchid mania,” imperialist business practices, and chromolithographic printmaking. Discussion of the transition of this collection into a museum art collection covers its …

Maternal Monitoring And Maternal Psychological Well-Being: Important Components In Treating Conduct Disorder, Benjamin Rosen

Theses and Dissertations

Conduct disorder is characterized by behaviors that take a large toll on the individuals, families, and communities afflicted. Thus, improving treatment effectiveness should be a high priority. Currently, common intervention programs do not address parental depression, even though it has been linked to adolescent conduct disorder behaviors in some studies. The current study assessed whether the relation between maternal depression and adolescent conduct disorder behaviors is mediated by another factor which has been linked to conduct disorder behaviors, maternal monitoring. Results did not support the hypothesized mediated association, but did show significant individual associations for both maternal depression and maternal …

Regulation Of Satiety Quiescence: Cyclic Gmp, Tgf Beta, And The Asi Neuron, Thomas Gallagher

Theses and Dissertations

The worm Caenorhabditis elegans is a well-studied model organism in numerous aspects of its biology. This small free living nematode has less than 1,000 cells, but shows clear conservation in both signaling and behavior to mammals in aspects of appetite control. This is of importance to humans, where failure of appetite control is a major factor in the unprecedented obesity epidemic that we see today. In general, worm behavior reflects its internal nutritional state and the availability and quality of food. Specifically, worms show a behavioral state that mimics aspects of the mammalian behavioral satiety sequence, which has been termed …

Spectral Analysis Of Nonstationary Heart Rate Of Neonates Receiving Therapeutic Hypothermia Treatment, Tareq Al-Shargabi

Theses and Dissertations

We studied Heart Rate Variability (HRV) evolution during therapeutic hypothermia in newborns with hypoxic ischemic encephalopathy (HIE) using spectral analysis. We hypothesized that HRV measures are predictive of neurological outcome in babies with HIE. Non-stationarity in the data causes inaccurate quantification of the spectral power. A modification was proposed to power spectral analysis approach to mitigate the effect of non-stationarity. The modified and the standard approaches were applied to cardiac beat-to-beat intervals of newborns receiving hypothermia treatment. The performance of the approaches in distinguishing the RRi dynamics of two groups of newborns was assessed using area under the receiver operating …