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Nicotinic Acetylcholine Receptor (Nachr) Dependent Chorda Tympani Taste Nerve Responses To Nicotine, Ethanol And Acetylcholine, Zuo Jun Ren, Shobha Mummalaneni, Jie Qian, Clive M. Baumgarten, John A. Desimone, Vijay Lyall

Physiology and Biophysics Publications and Presentations

Nicotine elicits bitter taste by activating TRPM5-dependent and TRPM5-independent but neuronal nAChR-dependent pathways. The nAChRs represent common targets at which acetylcholine, nicotine and ethanol functionally interact in the central nervous system. Here, we investigated if the nAChRs also represent a common pathway through which the bitter taste of nicotine, ethanol and acetylcholine is transduced. To this end, chorda tympani (CT) taste nerve responses were monitored in rats, wild-type mice and TRPM5 knockout (KO) mice following lingual stimulation with nicotine free base, ethanol, and acetylcholine, in the absence and presence of nAChR agonists and antagonists. The nAChR modulators: mecamylamine, dihydro-β-erythroidine, and …

An Opioid-Like System Regulating Feeding Behavior In C. Elegans, Mi Cheong Cheong, Alexander B. Artyukhin, Yong-Jai You, Leon Avery

Physiology and Biophysics Publications and Presentations

Neuropeptides are essential for the regulation of appetite. Here we show that neuropeptides could regulate feeding in mutants that lack neurotransmission from the motor neurons that stimulate feeding muscles. We identified nlp-24 by an RNAi screen of 115 neuropeptide genes, testing whether they affected growth. NLP-24 peptides have a conserved YGGXX sequence, similar to mammalian opioid neuropeptides. In addition, morphine and naloxone respectively stimulated and inhibited feeding in starved worms, but not in worms lacking NPR-17, which encodes a protein with sequence similarity to opioid receptors. Opioid agonists activated heterologously expressed NPR-17, as did at least one NLP-24 peptide. Worms …

The Effects Of Computer-Assisted Instruction On The Mathematics Achievement Of Students With Emotional And Behavioral Disorders, Kenya Williams

Theses and Dissertations

Mathematics is essential in everyday life activities and most educational opportunities and careers require mathematical knowledge, thus it is vital that students with emotional and behavioral disorders (EBD) receive sufficient instruction that leads to proficiency in the subject. Performing poorly in mathematics can result in dire future outcomes. This is particularly true for students with EBD, who already experience significant difficulties throughout and after their educational career. While studies have documented the academic and behavioral problems of students with EBD, not until recently have studies begun to concentrate on academic interventions that may aid in preventing some of the academic …

Finding Unfound_Graphic Hybridity, Yaeyoung Park

Theses and Dissertations

Hybridity is the result when visual form, color or tools interact. While not every combination of multiple elements result in success, I believe creativity, intuition and serendipity determines the successful hybridity. This is the documentation of my journey to develop a personal definition of successful hybridity in graphic design.

Proof-Of-Concept Of Environmental Dna Tools For Atlantic Sturgeon Management, Jameson Hinkle

Theses and Dissertations

Abstract

The Atlantic Sturgeon (Acipenser oxyrinchus oxyrinchus, Mitchell) is an anadromous species that spawns in tidal freshwater rivers from Canada to Florida. Overfishing, river sedimentation and alteration of the river bottom have decreased Atlantic Sturgeon populations, and NOAA lists the species as endangered. Ecologists sometimes find it difficult to locate individuals of a species that is rare, endangered or invasive. The need for methods less invasive that can create more resolution of cryptic species presence is necessary. Environmental DNA (eDNA) is a non-invasive means of detecting rare, endangered, or invasive species by isolating nuclear or mitochondrial DNA (mtDNA) from the …

Starvation-Induced Collective Behavior In C. Elegans, Alex B. Artyukhin, Joshua J. Yim, Mi Cheong Cheong, Leon Avery

Physiology and Biophysics Publications and Presentations

We describe a new type of collective behavior in C. elegans nematodes, aggregation of starved L1 larvae. Shortly after hatching in the absence of food, L1 larvae arrest their development and disperse in search for food. In contrast, after two or more days without food, the worms change their behavior—they start to aggregate. The aggregation requires a small amount of ethanol or acetate in the environment. In the case of ethanol, it has to be metabolized, which requires functional alcohol dehydrogenase sodh-1. The resulting acetate is used in de novo fatty acid synthesis, and some of the newly made …

Addition Of Interleukin-21 For Expansion Of T-Cells For Adoptive Immunotherapy Of Murine Melanoma, Christine Kathryn Zoon, Wen Wan, Laura Graham, Harry D. Bear

Surgery Publications

We previously demonstrated that interleukin (IL)-7/15 was superior to IL-2 for expansion of T cells in vitrofor adoptive immunotherapy. We sought to ascertain whether IL-21 would further improve yield and therapeutic efficacy of T cells in culture. Naïve T cell receptor (TcR) transgenic splenocytes or antigen-sensitized lymph node cells were harvested from PMEL-1 mice and exposed to bryostatin-1 and ionomycin (B/I) for 18 h. Cells were then cultured in IL-2, IL-21, IL-7/15 or IL-7/15/21 for six days. Harvested cells were analyzed by flow cytometry and used to treat C57Bl/6 mice injected intravenously with B16 melanoma. Lungs were harvested and …

New Vistas In Science Education, K. Waite, B. Healy

Journal of Mathematics and Science: Collaborative Explorations

In the summer of 2012, a colleague and I attended the four-week Virginia Initiative for Science Teaching and Achievement (VISTA) Elementary Summer Science Institute where we were trained to conduct inquiry-based science teaching in a problem-based learning setting. We then implemented our training in our own academic classrooms by developing a Problem-Based Learning unit meeting the objectives of our Virginia standards-based science curriculum and selecting a topic with tics to our local community. Toward demonstrating that students, teachers, and educational systems stand to benefit from the implementation of this methodology, this article clarifies the following aspects: 1) outlines the problem, …

Analytical Method Development For The Detection And Analysis Of Protein Carbonyls, Chelsea M. Coffey

Theses and Dissertations

Oxidative stress can result in changes to many biomolecules and also affect their activities. We are interested in protein carbonylation, a type of unnatural oxidation which has been associated with numerous degenerative disease states and is also a consequence of the natural aging process. Protein carbonyls are stable species, but countless analytical barriers exist in terms of their identification. Thus, the main goal of this work was to develop and optimize analytical methods that could be used to help us better understand which, where, and how proteins are being carbonylated.

Initial studies involved method validation for carbonylating, tagging, and enriching …

Bracquemond, Ruskin, The Haviland-Hayes Service, And Rookwood: Japonisme And Permanence In Art Pottery, Emily G. Campbell

Theses and Dissertations

There are two principle arguments in this thesis. First, this thesis will show that Félix Bracquemond had a profound impact on late-nineteenth-century and early-twentieth-century ceramics in America. Second, this thesis will illustrate how John Ruskin’s principle that pottery is “more permanent than the Pyramids” encouraged reform of the ceramic arts and shaped the Art Pottery Movement of the late nineteenth century. After this thesis introduces Bracquemond as an innovator in ceramic decoration and the dissemination of Ruskin’s principle, the thesis will examine two instances in the American Art Pottery Movement in which Bracquemond’s and Ruskin’s influence can be detected. The …

The Slc22 Transporter Family: Novel Insights To Roles In Drug Efficacy, Drug-Drug Interactions And Mood Disorders, Xiaolei Pan

Theses and Dissertations

Numerous studies have demonstrated the impact of organic cation (OCTs; SLC22 family) and anion transporters (OATs; SLC22 family) on the efficacy and safety of clinically important therapeutics. To be specific, OCTs and OATs have been identified as determinants for uptake into and secretion from enterocytes, hepatocytes and renal proximal tubular cells, and are frequent sites of drug-drug interaction (DDI). In addition, OCTs expressed in brain are components of the low-affinity, high capacity clearance pathway (uptake-2) for biogenic monoamine neurotransmitters. As a result, OCTs may represent novel targets for mood disorders.

The inhibitory effects of several therapeutic agents, designed drugs and …

The Effect Of Optogenetic Manipulation Of Ss Interneurons Within Malformed, Epileptogenic Cortex, Nicole Ekanem

Theses and Dissertations

A large percentage of individuals with intractable epilepsies have an accompanying cortical malformation, the underlying cellular mechanisms of which are poorly understood. It is known however that in an animal model for one such malformation, polymicrogyria, epileptogenesis occurs most easily from an adjacent area termed the paramicrogyral region (PMR). Previous studies implicate SS interneurons as a potential contributor to this pathology, which lead to our hypothesis: in PMR, SS interneurons exert a higher modulatory influence on excitatory pyramidal cells, as compared to the same by SS interneurons within homologous control cortex.

Using a freeze-lesion model for polymicrogyria in transgenic mice …

Characterization Of Mechanism Of Action Of Hydrogen Sulfide (H2s) In The Regulation Of Smooth Muscle Function, Ancy D. Nalli

Theses and Dissertations

Hydrogen sulfide (H₂S) is receiving increasing interest, as much as nitric oxide (NO) and carbon monoxide have received previously, to understand its physiological functions as it meets all the criteria to define as a third gasotransmitter. Endogenous synthesis from L-cysteine via cystathionine-γ-lyase (CSE) and cystathionine-β-synthase (CBS) and the function of H₂S as an inhibitor of smooth muscle contraction in gastrointestinal tract are known. However, the loci of generation and action of H₂S, and the mechanism of inhibition of contraction are unknown. Hence, my aims in the present study are to: i) identify the expression …

Regulation Of Tie2 Extracellular Complex Formation In Angiogenesis, Annamarie Dalton

Theses and Dissertations

Pathological angiogenesis is an essential component of tumor growth, development, and metastasis for which few effective therapeutic options exist. Though many cancer therapies target the function of cell surface receptors, mechanisms regulating membrane receptor crosstalk remain unclear. Two important families of receptors in angiogenesis, the Ties and Integrins, respond to the extracellular environment via outside-in and, in the case of Integrins, also inside- out signaling. Recent reports showed that the endothelial specific tyrosine kinase receptor, Tie2, forms complexes with two of the endothelial Integrin heterodimers, α₅β₁ and α_Vβ₃, providing a convenient mechanism for …

Mitochondrial Biogenesis And Electrical Properties Of Hpsc-Derived Motor Neurons, Laura O'Brien

Theses and Dissertations

Human pluripotent stem cells (hPSCs), including human embryonic stem cells (hESCs) and induced pluripotent stem cells (iPSCs) hold great promise in the fields of drug development and regenerative medicine. If iPSCs reprogrammed from patient cells replicate what is seen in vivo they may be used as a model of disease. A process that is disrupted in many neurodegenerative diseases is mitochondrial biogenesis. One of these diseases is amyotrophic lateral sclerosis (ALS), which is characterized by loss of motor neurons in the brain and spinal cord. Differentiation of hPSCs into motor neurons offers a way to study a previous unavailable cell …

Into The Vortex Of A Maelstrom: The Art Of Municipal Governance In Confederate Richmond, Joshua Shaffer

Theses and Dissertations

From May 1861 until April 1865 the city of Richmond, Virginia served as the capital of the Confederate States of America, during the American Civil War. Throughout the course of the war it operated alongside the established governments of the Commonwealth of Virginia, the County of Henrico, and Richmond City. The body that experienced the greatest fluctuation and change was the municipal government, which consisted of a city council, mayor, and hustings court. The city government faced existential challenges that included an increase in its population, an influx of Confederate soldiers, and the constant threat of the Union army. While …

Elucidating The Role Of Endogenous Electric Fields In Regulating The Astrocytic Response To Injury In The Mammalian Central Nervous System, Matthew L. Baer

Theses and Dissertations

Endogenous bioelectric fields guide morphogenesis during embryonic development and regeneration by directly regulating the cellular functions responsible for these phenomena. Although this role has been extensively explored in many peripheral tissues, the ability of electric fields to regulate wound repair and stimulate regeneration in the mammalian central nervous system (CNS) has not been convincingly established. This dissertation explores the role of electric fields in regulating the injury response and controlling the regenerative potential of the mammalian CNS. We place particular emphasis on their influence on astrocytes, as specific differences in their injury-induced behaviors have been associated with differences in the …

The Fatal Lamp And The Nightmare After Christmas: The 1811 Richmond Theatre Fire, Amber Marie Martinez

Theses and Dissertations

ABSTRACT

THE FATAL LAMP AND THE NIGHTMARE AFTER CHRISTMAS: THE RICHMOND THEATRE FIRE OF 1811

By Amber Marie Martinez, Bachelor of Fine Arts in Theatre Performance

A thesis submitted in partial fulfillment of the requirements for the degree of Master of Fine Arts in Theatre Pedagogy at Virginia Commonwealth University.

Virginia Commonwealth University, 2015

Director: Dr. Noreen C. Barnes, Director of Graduate Studies, Department of Theatre

“How strange a preface the loud laughter excited by a pantomime, to volumes of smoke and fire” (The American Standard, 27 December 1811). Building fires were not exactly uncommon back in the …

Axon Initial Segment Stability In Multiple Sclerosis, Suneel K. Thummala

Theses and Dissertations

Multiple sclerosis (MS) is an autoimmune disease of the central nervous system characterized by inflammation and demyelination. In addition to these hallmark features, MS also presents with axonal pathology, which is likely responsible for the signs and symptoms of the disease. Although prominent in MS, axonal pathology is frequently considered a consequence of demyelination and not a primary event. This conclusion is consistent with demyelination inducing the loss of specific axonal domains, known as the nodes of Ranvier that are responsible for the propagation of action potentials along the axon. In contrast, we propose that axonal pathology associated with MS …

Mysteries Of The Trypanosomatid Maxicircles: Characterization Of The Maxicircle Genomes And The Evolution Of Rna Editing In The Order Kinetoplastida, Preethi Ranganathan Iyengar

Theses and Dissertations

The trypanosomatid protists belonging to Order Kinetoplastida are some of the most successful parasites ever known to mankind. Their extreme physiological diversity and adaptability to different environmental conditions and host systems make them some of the most widespread parasites, causing deadly diseases in humans and other vertebrates.

This project focuses on their unique mitochondrion, called the kinetoplast, and more specifically involves the characterization of a part of their mitochondrial DNA (also called kinetoplast DNA or kDNA), the maxicircles, which are functional homologs of eukaryotic mitochondrial DNA in the kinetoplastid protists. We have sequenced and characterized the maxicircle genomes of 20 …

Role Of Extracytoplasmic Rna Polymerase Sigma 70 Factor, Pg0214, In The Survival Of Porphyromonas Gingivalis And In Adaptation To Environmental Stress., David M. Smith

Theses and Dissertations

Porphyromonas gingivalis, a gram-negative anaerobic, pathogenic bacterium is a major etiological agent in the initiation and progression of periodontal disease. Due to the ever-changing environment of the oral cavity, inhabitants like Porphyromonas gingivalis must possess the ability to adapt to changes in environmental conditions like pH, temperature, oxygen tension, and metal concentration. P. gingivalis should therefore have an efficient regulatory system in order to adapt and survive in the oral cavity. This response adaptation occurs at the transcriptional level, which involves alternative sigma factors. Extracytoplasmic function sigma (ECF-s) factors are the largest group of alternative sigma factors that play …

The Effects Of Hydropeaking On Lotic Benthic Macroinvertebrate Assemblages, Andrew L. Garey

Theses and Dissertations

The term hydropeaking refers to anthropogenically induced, short-duration, high-magnitude discharge pulses that are generated in lotic systems for electricity production. The practice of hydropeaking produces the largest source of renewable energy worldwide, and its use is projected to increase through the year 2040. The primary objective of this work was to evaluate the effects of hydropeaking on benthic macroinvertebrate assemblages, which are important components of lotic ecosystems. Results of this work show that, across a wide range of impacted systems worldwide, the consistently observed patterns of elevated benthic macroinvertebrate drift in response to hydropeaking pulses are primarily related to the …

Belt Melon Grass, Andrew M. Francis

Theses and Dissertations

This essay was written largely after the completion of my thesis exhibition which shares its title. An integral aspect of the work was the after-­hours maintenance it required. Below I describe the unforeseen personal significance that labor came to hold and the way in which it functioned as a healing ritual. Through this work, and those leading up to it, I have a reinvigorated awareness of the importance of therapy as an aspect of my art­making, of which this thesis is a testament.

Speed And Resolution In The Age Of Technological Reproducibility, Shawn Taylor

Theses and Dissertations

The rate of acceleration of the biologic and synthetic world has for a while now, been in the process of exponentially speeding up, maxing out servers and landfills, merging with each other, destroying each other. The last prehistoric relics on Earth are absorbing the same oxygen, carbon dioxide and electronic waves in our biosphere as us. A degraded .jpeg enlarged to full screen on a Samsung 4K UHD HU8550 Series Smart TV - 85” Class (84.5” diag.). Within this composite ecology, the ancient limestone of the grand canyon competes with the iMax movie of itself, the production of Mac pros, …

The Richmond Maker Museum: The Evolution Of Process, Erin E. Casey

Theses and Dissertations

The Richmond Maker Museum is a working museum design, offering an inside look at past achievements, juxtaposed with the unlimited future possibilities of an evolving, active maker culture. It is a dynamic place designed to allow makers to showcase skills, take risks, engage the public, and grow their craft in real time. The museum displays finished pieces, introduces makers, demonstrates the processes they employ in their work, and invites the community to meet the artisans who, through skill, ingenuity, and hard work, make the artifacts on display. This type of educational museum experience does not currently exist on this scale …

Forbearance Across Culture, Yin Lin

Theses and Dissertations

Forbearance is defined for the purpose of the present dissertation as, “the attempt to suppress the visible signs of negative emotion (i.e., emotional expression) and visible behaviors (i.e., the expression of negative vengeful or avoidant motives) in response to a hurt or offense, often (but not always) for the sake of group harmony.” I reviewed the literature on forbearance and cross-cultural studies in suppression of emotion or emotional expression under the framework of New Big Five personality processes and cultural models of self and relating. Then I offered three propositions about forbearance. Furthermore, I collected three samples and …

Elaboration And Design Of Α7 Nachr Negative Allosteric Modulators, Osama I. Alwassil

Theses and Dissertations

α7 Neuronal nicotinic acetylcholine receptors are one of two major classes of receptors responsible for cholinergic neurotransmission in the central nervous system. The existence of α7 neuronal nAChRs in different regions of the nervous system suggests their involvement in certain essential physiological functions as well as in disorders such as Alzheimer’s disease (AD), drug dependence, and depression. This project was aimed toward the discovery and development of small–molecule arylguanidines that modulate α7 nAChR function with improved subtype-selectivity through an allosteric approach. Identifying the required structural features of these small molecules allowed optimization of their negative allosteric modulator (NAM) actions at …

A Comparison Of Vitamin D Levels In Children With Early Childhood Caries, Vanessa O. Hofilena

Theses and Dissertations

Purpose: To determine if there is an association between early childhood caries (ECC) and vitamin D deficiency, as measured via a serum sample. An exploratory goal was to investigate the possibility of measuring vitamin D with a salivary assay. Methods: Serum samples of patients who were scheduled for a dental or otolaryngology procedure under general anesthesia were assessed for 25-hydroxyvitamin D (25(OH)D), parathyroid hormone and calcium. Results: Our analysis indicates that the vitamin D levels of the controls and ECC group were significantly different. There were significant inverse correlations between: 1) PTH levels and vitamin D and 2) decayed, missing, …

Poictesme: An Anthology Of Literature And Art (2015)

Pwatem (Poictesme), 2006-

Poictesme (now Pwatem) is an anthology of literature and art from undergraduate students at Virginia Commonwealth University. It publishes poetry, prose and art of all kinds from talented undergraduate students of all majors.

The End Of Prisons: Reflections From The Decarceration Movement, Carmen Lanos Williams

Journal of Hip Hop Studies

Book review of The End of Prisons: Reflections from the Decarceration Movement, edited by Mechtild E. Nagel and Anthony J. Nocella (2013).