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Kisspeptins - Expression, Purification, And Biological Assays, Mathew Puthayathu Kurian May 2013

Kisspeptins - Expression, Purification, And Biological Assays, Mathew Puthayathu Kurian

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Kisspeptins are hormones involved in the onset of puberty in adolescents and maintenance of reproductive function in adults. Defects in kisspeptin signaling are associated with many reproductive disorders. Kisspeptins were originally discovered as metastasis suppressors and have been found to suppress the metastasis of certain cancers. Unfortunately, many aspects of kisspeptin action at a cellular level remain unknown. Kisspeptins, therefore, are attractive candidates for large-scale production for use as research and therapeutic reagents. We developed a simple and cost effective method to express and purify kisspeptins in large quantities. We also tested the biological activity of kisspeptins on cells expressing …


Investigation Of The Oxidation/Reduction Of Prmt1, Substrate Interaction With Prmt1, And The Role Of Arginine Methylation In Rna Surveillance, Damon V. Nitzel May 2013

Investigation Of The Oxidation/Reduction Of Prmt1, Substrate Interaction With Prmt1, And The Role Of Arginine Methylation In Rna Surveillance, Damon V. Nitzel

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Enzymes are the machines of our cells. Just like machines, it takes a lot of energy to create them, and they then serve only the function they were created for. If we want to change the function of a machine, we need to modify it. Similarly, enzymes can be modified after their creation to give them additional function. These modifications can do a variety of things including activating (on) or inactivating (off) an enzyme, changing the enzyme’s location in the cell, and targeting the enzyme for destruction. This thesis focuses on a single class of enzymes, protein arginine methyltransferases (PRMTs), …


Assessing Vehicle-Related Mortality Of Mule Deer In Utah, Daniel D. Olson May 2013

Assessing Vehicle-Related Mortality Of Mule Deer In Utah, Daniel D. Olson

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Roads are essential in modern societies, but as populations grow and traffic volumes rise, roads will continue to be built and expanded. As a result, the effects that roads have on wildlife will likely intensify, making it imperative that managers understand those effects so mitigation can be directed accordingly. In Utah, considerable areas of mule deer (Odocoileus hemionus) habitat have been bisected by roads. Mule deer are commonly involved in vehicle collisions and there is concern that roads and vehicle traffic are impacting populations. This project was conducted to determine the number and demographic effects of deer-vehicle collisions, …


Oxidative Aliphatic Carbon-Carbon Bond Cleavage Reactions, Caleb J. Allpress May 2013

Oxidative Aliphatic Carbon-Carbon Bond Cleavage Reactions, Caleb J. Allpress

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

The work presented in this dissertation has focused on the activation and cleavage of chemical bonds between two carbon atoms. The selective oxidative activation of carbon-carbon bonds is important due to potential applications in the utilization of biomass for fuel production, applications in wastewater treatment and bioremediation, and in developing new reactions for organic synthesis of fine chemicals including pharmaceuticals. Ideally these reactions would be carried out with high atom economy at low temperatures and pressures, and using earth-abundant elements as reagents and catalysts. With these points in mind, nature provides an ideal model framework, carrying out its chemistry at …


Cationic Starch Synthesis, Development, And Evaluation For Harvesting Microalgae For Wastewater Treatment, Renil John Anthony May 2013

Cationic Starch Synthesis, Development, And Evaluation For Harvesting Microalgae For Wastewater Treatment, Renil John Anthony

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Microalgae are the preferred crop for the production of biodiesel. Microalgae are microplants that have the ability to harness sunlight more efficiently than other plants and store 20-80% lipids per g of dry algae in their cells. Microalgae have the extraordinary ability to grow in brackish water or wastewater. Microalgae can be grown in municipal wastewater to uptake phosphorus and nitrogen and remediate the wastewater of these nutrients. Microalgae thus cultivated accomplishes a dual role of wastewater treatment and provides a sustainable feedstock for biofuels and other bioproducts.

This study focused on efficiently harvesting microalgae from water using modified starch. …


Dot Product Graphs And Their Applications To Ecology, Sean Bailey May 2013

Dot Product Graphs And Their Applications To Ecology, Sean Bailey

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

We will introduce a new tool to visualize the comparison between different birds. This tool will allow users to use any number of measurable traits to see relationships between different birds, both individually and collectively.


Causes And Consequences Of Local Variability In Aroga Websteri Clarke Abundance Over Space And Time, Virginia L.J. Bolshakova May 2013

Causes And Consequences Of Local Variability In Aroga Websteri Clarke Abundance Over Space And Time, Virginia L.J. Bolshakova

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Sagebrush steppe habitat is rapidly declining in the Intermountain West in both quality and quantity. Observed reductions in animal and bird populations associated with sagebrush, invasion of exotic annual grasses such as cheatgrass (Bromus tectorum), and other concerns with climate change have led to a growing awareness of the need to monitor and better understand the rate of habitat loss. Sagebrush lands are subject to periodic loss caused by insect feeding damage, and human activities have the potential to promote unnaturally high levels of such loss. The sagebrush defoliating Aroga moth, Aroga websteri Clarke (Lepidoptera: Gelechiidae), can kill …


A Comparison Of Resistance To Extinction Following Dynamic And Static Schedules Of Reinforcement, Andrew R. Craig May 2013

A Comparison Of Resistance To Extinction Following Dynamic And Static Schedules Of Reinforcement, Andrew R. Craig

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Behavioral momentum theory states that the Pavlovian stimulus-reinforcer relation governs resistance to extinction. Thus, higher baseline reinforcer rates should produce more persistent behavior than lower baseline reinforcer rates. Though this positive relation generally is observed when behavior is maintained and subsequently disrupted in multiple schedules, the opposite relation is observed when single schedules are used. An alternative framework of extinction performance based on Bayesian change detection may be applied intuitively to describe resistance to extinction in single schedules of reinforcement. This approach asserts that detection of changes in reinforcer rates during extinction should be easier following training with rich reinforcer …


Vital Rates, Population Trends, And Habitat-Use Patterns Of A Translocated Greater Sage-Grouse Population: Implications For Future Translocations, Orrin V. Duvuvuei May 2013

Vital Rates, Population Trends, And Habitat-Use Patterns Of A Translocated Greater Sage-Grouse Population: Implications For Future Translocations, Orrin V. Duvuvuei

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Greater sage-grouse (Centrocercus urophasianus; sage-grouse) populations have declined range-wide with some local populations exhibiting dramatic decreases. In 2009–2010, radio-marked hens (30 hens in 2009 and 30 hens in 2010) were translocated from Parker Mountain in south-central Utah to augment a rapidly declining population on Anthro Mountain in northeastern Utah. Thirty-two resident female sage-grouse on Anthro Mountain were also radio-collared from 2009–2012.

I compared population vital rates (i.e., nest and brood success, and survival) of resident and translocated hens in Anthro Mountain’s population to those of a translocated population in Strawberry Valley, Utah and the source population. Of these …


Integrated Management Of Downy Brome (Bromus Tectorum L.) Infested Rangeland, Heather Elwood May 2013

Integrated Management Of Downy Brome (Bromus Tectorum L.) Infested Rangeland, Heather Elwood

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Invasive weed species are a threat to the health and functionality of many rangeland systems. Downy brome (Bromus tectorum) is an invasive annual grass that affects the productivity of rangelands by decreasing the grazing capacity for livestock as well as altering the wildfire cycle and competing against more desirable vegetation for limited resources.

In 2006, an Invasive Plant Management Plan and Environmental Assessment was approved for Dinosaur National Monument, calling for prioritization of invasive species management on high value wildlife habitat, vector areas, and for species with a high ecological impact. The Cub Creek Watershed was identified as a priority …


Ecology Of Coyotes On The Valles Caldera National Preserve, New Mexico: Implications For Elk Calf Recruitment, Suzanne J. Gifford May 2013

Ecology Of Coyotes On The Valles Caldera National Preserve, New Mexico: Implications For Elk Calf Recruitment, Suzanne J. Gifford

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Valles Caldera National Preserve (VCNP) managers were concerned about low elk recruitment observed at the same time as an apparent increase in sightings of coyotes and observations of coyote predation on elk calves. The goal of this study was to describe coyotes’ ecological interactions with elk, particularly coyote diet and movements on the Valle Grande, a large grassland meadow in the southeastern portion of the VCNP.

We examined coyote diet by quantifying undigested remains of food items in coyote scats (feces). The most frequent taxa were rodents (montane voles and pocket gophers), elk (adult and calf), insects (grasshoppers and beetles), …


Structural And Functional Characterization Of The Ldp3 (Vhz) Protein Tyrosine Phosphatase, Vyacheslav I. Kuznetsov May 2013

Structural And Functional Characterization Of The Ldp3 (Vhz) Protein Tyrosine Phosphatase, Vyacheslav I. Kuznetsov

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Protein phosphorylation is as an important regulatory mechanism that controls a large number of physiologically important cellular processes. Proteins are phosphorylated on aliphatic (serine and threonine) as well aromatic (histidine and tyrosine) residues predominantly located on sterically accessible loops. Phosphorylation affects three- dimensional structures of proteins resulting in conformational changes which activate or deactivate enzymatic functions. Because of the ability of protein phosphorylation to modulate a multitude of diverse cellular processes, it became an appealing and promising target for drug therapy.

Attachment of phosphate is accomplished by kinases, whereas the removal of the phosphoryl group is performed by protein phosphatases. …


A Longitudinal Study Of English Narrative Discourse Development In Young Spanish-English Bilinguals, Abbie Olszewski May 2013

A Longitudinal Study Of English Narrative Discourse Development In Young Spanish-English Bilinguals, Abbie Olszewski

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Producing oral narratives is the best predictor of later literacy functioning. The ability to use performance on oral narratives as a way to identify children who may be at- risk for academic and language problems is helpful for educators. For example, it is likely that children who are identified with language impairments or who are learning English as a second language may have difficulty creating narratives due to the inherent language complexity of creating narratives. Research has demonstrated that similar to English monolingual children, narrative ability has been shown to underlie literacy development for bilingual children. Although there are numerous …


Winter Ecology Of Waterfowl On The Great Salt Lake, Utah, Josh L. Vest May 2013

Winter Ecology Of Waterfowl On The Great Salt Lake, Utah, Josh L. Vest

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

I designed a suite of studies in coordination with Utah Division of Wildlife Resources (UDWR) to evaluate waterfowl use of the GSL in winter and ecological aspects associated with GSL use. These studies provided insight into key information gaps previously identified by UDWR regarding management of GSL resources. Population surveys indicated total duck abundance was low when GSL surface elevations were low and wetland resources diminished because of persistent drought in the system. Also, ducks appear to use hypersaline parts of GSL more when freshwater habitats are limited from either drought or ice conditions. Common goldeneye, northern shoveler, and green-winged …


Emotional Intelligence In Medical Laboratory Science, Travis Miles Price May 2013

Emotional Intelligence In Medical Laboratory Science, Travis Miles Price

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Medical laboratory scientists (MLSs) are responsible for performing highly complex biochemical analyses on samples collected from patients in hospitals and clinics. The performance of these tests often involves little patient exposure on the part of the MLS and as a result some have wondered about the role soft skills, like communication, conflict resolution and empathy, play in medical laboratory science. The aim of this study was to explore the importance that medical laboratory administrators place on these types of skills, collectively referred to as emotional intelligence (EI), as well as to assess how satisfied they were with the demonstration of …


Regulation And Expression Of Nanog, Oct4, And Sox2 In The Bovine Blastocyst Following Somatic Cell Nuclear Transfer, Justin Scott Hall May 2013

Regulation And Expression Of Nanog, Oct4, And Sox2 In The Bovine Blastocyst Following Somatic Cell Nuclear Transfer, Justin Scott Hall

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

The Animal, Dairy, and Veterinary Sciences Department (ADVS) and the Center for Integrated Biosystems (CIB) at Utah State University are studying various molecular mechanisms involved in the animal cloning process. This study involves the extensive network of people, facilities, equipment, and funding already associated with the CIB and ADVS joint project.

Cloning involves many molecular challenges that for the most part have become roadblocks for the normal development of the fetus. The mechanisms necessary to transform an adult cell into a competent stem cell that can then transform and develop into a healthy organism are poorly understood. Some of these …


Analysis Of Single Nucleotide Polymorphism Panels For Bovine Dna Identification, Kimberly A. Blanchard May 2013

Analysis Of Single Nucleotide Polymorphism Panels For Bovine Dna Identification, Kimberly A. Blanchard

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Single nucleotide polymorphisms (SNPs) have great power in resolving questions of unknown parentage on ranches or dairies where errors could have been made in pedigree record keeping or when such records were simply not kept. Currently a panel of 88 SNPs based on a panel originally created by USDA-MARC is commercially available from the company Fluidigm®. Our objective was to determine whether or not the number of SNPs could be reduced to form a smaller, more cost-efficient parentage-testing panel. A smaller panel would be beneficial to farmers and researchers alike in the reduction of time spent running and …


Attentional Factors In Temporal Distortion: The Effects Of Food Availability On Responses Within The Interval Bisection Task, Robert N. Johnson May 2013

Attentional Factors In Temporal Distortion: The Effects Of Food Availability On Responses Within The Interval Bisection Task, Robert N. Johnson

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Time estimation is a process that underlies many complex behaviors. Accurately timed performance depends upon some biological mechanism, which may be revealed by experimental methods. There have been several proposals on the subject of the function of the timing mechanism, some of which have revealed contradictory findings regarding the effect of distraction on timing. Whereas some studies have revealed underestimation (reports of time moving more slowly than it actually is) of the target interval following distracter presentation, others have revealed generalized disruption (no meaningful evidence of timing). The purpose of the present study was to determine whether food presentation for …


Modeling Usa Stream Temperatures For Stream Biodiversity And Climate Change Assessments, Ryan A. Hill May 2013

Modeling Usa Stream Temperatures For Stream Biodiversity And Climate Change Assessments, Ryan A. Hill

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Stream temperature in one of the most biologically important aspects of water quality, but we lack temperature information for the vast majority of streams within the USA. Stream temperature can be influenced by several types of landscape and waterway alteration including upstream urbanization, agriculture, and reservoir releases. Stream temperatures are also expected to be affected by climate change over the next
century. We need to know how stream temperatures vary naturally, how they are influenced by human activity, and how they will respond to climate changes to effectively manage stream ecosystems. I used data from several thousand streams within the …


An Environmental History Of The Bear River Range, 1860-1910, Bradley Paul Hansen May 2013

An Environmental History Of The Bear River Range, 1860-1910, Bradley Paul Hansen

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

The study of environmental history suggests that nature and culture change all the time, but that the rate and scale of such change can vary enormously. During the late 19th and early 20th centuries, Anglo settlement in the American West transformed landscapes and ecologies, creating new and complex environmental problems. This transformation was particularly impressive in Cache Valley, Utah's Bear River Range. From 1860 to 1910, Mormon settlers overused or misused the Bear River Range's lumber, grazing forage, wild game, and water resources and introduced invasive plant and animal species throughout the area.

By the turn of the 20th century, …


Evaluating Eriogonum Corymbosum Tolerance To Frequent Irrigation And Evaluating Its Significant Morphological Variations For Potential Cultivars, Graham C. Hunter May 2013

Evaluating Eriogonum Corymbosum Tolerance To Frequent Irrigation And Evaluating Its Significant Morphological Variations For Potential Cultivars, Graham C. Hunter

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Eriogonum corymbosum (Lacy Buckwheat) is an attractive subshrub species native to low rainfall areas of the Colorado Plateau and suitable for low water landscapes in the Intermountain West (IMW). Low water use landscapes can contribute to water conservation in arid climates; developing a palette of plants that are both attractive and drought tolerant can promote the acceptance of low water use landscapes as an alternative to the traditional bluegrass landscapes of the IMW. In 2007 a strip plot design containing four repetitions with four randomly assigned plants each of Eriogonum corymbosum, Eriogonum thompsoniae, and the control species Cornus …


Development Of The Multiple Use Plug Hybrid For Nanosats (Muphyn) Miniature Thruster, Shannon Dean Eilers May 2013

Development Of The Multiple Use Plug Hybrid For Nanosats (Muphyn) Miniature Thruster, Shannon Dean Eilers

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

The Multiple Use Plug Hybrid for Nanosats (MUPHyN) prototype thruster incorporates solutions to several major challenges that have traditionally limited the deployment of chemical propulsion systems on small spacecraft. The MUPHyN thruster offers several features that are uniquely suited for small satellite applications. These features include 1) a non-explosive ignition system, 2) non-mechanical thrust vectoring using secondary fluid injection on an aerospike nozzle cooled with the oxidizer flow, 3) a non-toxic, chemically-stable combination of liquid and inert solid propellants, 4) a compact form factor enabled by the direct digital manufacture of the inert solid fuel grain. Hybrid rocket motors provide …


Clonal Diversity Of Quaking Aspen (Populus Tremuloides): How Multiple Clones May Add To Theresilience And Persistence Of This Forest Type, Richard Scott Gardner May 2013

Clonal Diversity Of Quaking Aspen (Populus Tremuloides): How Multiple Clones May Add To Theresilience And Persistence Of This Forest Type, Richard Scott Gardner

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Aspen forests and woodlands are widespread across the western United States and are a primary component of many ecosystems in the west. Aspen is a clonal species, with reproduction occurring both by root sprouting (suckering) and seeding. Traditionally, western aspen forests were thought to consist almost entirely of large clones established several thousands of years ago, with seeding events being rare and ecologically negligible. Although clones in the western US can grow to be many acres, recent studies have demonstrated a far greater proportion of small clones than had been previously thought to exist. In this study I wanted to …


Predictors Of Father-Child And Mother-Child Attachment In Two-Parent Families, Jared P. Benware May 2013

Predictors Of Father-Child And Mother-Child Attachment In Two-Parent Families, Jared P. Benware

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Jared Benware, M.S. and Gretchen Gimpel Peacock, Ph.D. at Utah State University examined parent-child attachment in two-parent families. Specifically, the relationship between mother-child and father-child attachment, the relationship between a child's temperament and parent-child attachment, and the relationship between the attachment and the amounts of time a parent spends away from his or her child. The researchers solicited parents as participants to complete questionnaires that measure parent-child attachment and the temperament of their child. This study focused especially on providing more information on the connection between father-child attachment and mother-child attachment rather than focusing only on mothers as the primary …


Enhanced Structural Support Of Metal Sites As Nodes In Metal-Organic Frameworks Compared To Metal Complexes, Sanjit Das May 2013

Enhanced Structural Support Of Metal Sites As Nodes In Metal-Organic Frameworks Compared To Metal Complexes, Sanjit Das

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Crystalline porous materials have gained long-standing interest for their application in gas storage, separation and catalysis. These materials have been useful for domestic, scientific and industrial purposes for many decades. Zeolites are a well known example of such materials.

Metal-organic frameworks are a new class of crystalline porous materials. They have many advantages over the more widely known zeolites. Though metal-organic frameworks are relatively new, their basic structure, known as the secondary building unit, very closely resembles the structure of metal complexes. Such metal complexes have been characterized in chemistry for more than a century.

Chemical catalysis is a process …


An Evaluation Of Current Stormwater Best Management Practice Relationships Between Design And Efficiency: A Series Of Local And National Case Studies, Amanda Ann Goodwin May 2013

An Evaluation Of Current Stormwater Best Management Practice Relationships Between Design And Efficiency: A Series Of Local And National Case Studies, Amanda Ann Goodwin

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Water quality continues to be threatened by human development activities such as stormwater runoff from urbanization. This study addresses the question of how stormwater Best Management Practice (BMP) system design choices affect pollutant removal efficiency, through the examination of 12 case study sites (across five states) that use three common BMP system design types (detention, retention, and wetland channel). Water quality information was obtained from the International Stormwater Database and site composite grab samples. Development conditions were inventoried by orthophotos and assessed using Geographic Information System (GIS) and AutoCAD data.

This study relates Total Suspended Solid (TSS) load removal efficiency …


Developing An Optimized Light Spectrum For Plant Growth And Development, Kevin Richard Cope May 2013

Developing An Optimized Light Spectrum For Plant Growth And Development, Kevin Richard Cope

Undergraduate Honors Capstone Projects

Light-emitting diodes (LEDs) are a rapidly developing technology for plant growth lighting and have become a powerful tool for understanding the spectral effects of light on plants. Several studies have shown that some blue light is necessary for normal growth and development, but the effects of blue light appear to be species dependent and may interact with other wavelengths of light as well as photosynthetic photon flux (PPF). Here we report the photobiological effects of three types of white LEDs (warm, neutral and cool) on the growth and development of radish, soybean, and wheat. All species were grown at two …


Development Of A Genetic Marker To Differentiate Between Pulex Irritans And Pulex Simulans, Colby Russell Kearl May 2013

Development Of A Genetic Marker To Differentiate Between Pulex Irritans And Pulex Simulans, Colby Russell Kearl

Undergraduate Honors Capstone Projects

Pulex irritans and Pulex simulans are zoonotic flea vectors of plague and other infectious diseases. P. irritans have historically been known to use carnivores as a host, while P. simulans primarily parasitizes omnivores. To fully understand arthropod-borne infectious disease transmissibility and potential for geographical spread, it is important to differentiate between these two flea species. Traditional taxonomy uses the flea's male morphological features to distinguish species. There are no observable morphological differences between the female P. irritans and P. simulans. Molecular markers of the internal transcribed spacer regions (ITS) have been successfully used in other insect organisms to differentiate …


The Southeast In Context: An Assessment Of The Trauma Associated With Agriculture, Martin Welker May 2013

The Southeast In Context: An Assessment Of The Trauma Associated With Agriculture, Martin Welker

Undergraduate Honors Capstone Projects

Hunter-gatherer tradition prevailed as the dominant subsistence pattern for most of human history. Between 9,000 and 13,000 years ago peoples in the Levant, New World, and Asia began the domestication and cultivation of wild flora and fauna, creating a subsistence pattern that subsequently spread to neighboring regions (Abbo et al. 2010; Bellwood 2009; Purugganan & Fuller 2009; Richerson et al. 2001). The influence of this agricultural transition on human populations is manifested in various forms in the human skeleton, many of which have received intensive study: dental caries, degenerative joint disease, decreased stature, and increased birth rates (Bridges 1991; Larson …


The First Struggles Of The Capitalistic Peace And Its Detractors: An Analysis Of Greek Trade And Its Effects On Foreign Policy In The Athenian State, Joshua Blume May 2013

The First Struggles Of The Capitalistic Peace And Its Detractors: An Analysis Of Greek Trade And Its Effects On Foreign Policy In The Athenian State, Joshua Blume

Undergraduate Honors Capstone Projects

Although, a ubiquitous number of articles has arisen recently debating the validity of the so-called "Capitalist Peace," there has been little exploration of the theory from an ancient historical perspective, primarily due to the lack of trade data between countries or city-states. As the primordial birthplace of trade and democracy, there is perhaps no better example than ancient Greece to shed insight into the possible validity of the capitalist peace theory. Utilizing new research involving trade in ancient Greece, this paper seeks to explore the relative validity of the "Capitalist Peace" in ancient Greece by conducting a qualitative analysis of …