Digital Commons Network^™

Observation Of Struvite In The Mixed Microalgae Biofilm Matrix Of A Rotating Algal Biofilm Reactor During Nutrient Removal From Municipal Anaerobic Digester Filtrate, Kyle M. Hillman

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Central Valley Water Reclamation Facility (CVWRF) in Salt Lake City is the largest municipal wastewater treatment plant in Utah and must meet new and rigorous nutrient effluent standards–over 95% reduction in phosphorus output by 2025. Filtrate from CVWRF anaerobic digesters contains high levels of nitrogen, phosphorus, and magnesium. Supersaturation of these constituents leads to nuisance struvite precipitation that clogs belts, pumps, and pipes downstream of anaerobic digesters. Struvite is a mineral precipitate composed of equimolar magnesium, ammonium, and phosphate. Controlled precipitation of struvite helps prevent clogging and scaling, removes phosphate from wastewater, and generates a marketable fertilizer product.

Struvite was …

Brussels Sprouts In The Garden, Dan Drost, Michael Johnson

All Current Publications

This fact sheet describes brussels sprouts in the garden, gives recommended varieties, how to grow, problems, harvest and storage, productivity, and nutrition.

Broccoli In The Garden, Dan Drost, Michael Johnson

All Current Publications

This fact sheet describes broccoli in the garden, gives recommended varieties, how to grow, problems, harvest and storage, productivity, nutrition, and frequently asked questions.

Cabbage In The Garden, Dan Drost, Mike Johnson

All Current Publications

No abstract provided.

Honoring The Class Of 2020, Usu College Of Engineering

College of Engineering News

You’ve accomplished something extraordinary. By persevering through years of challenging work and training, you have earned one of the most versatile and valuable college degrees. In the last few months, you have shown tremendous agility in navigating an unprecedented public health crisis while completing a rigorous academic program and senior capstone requirements. You overcame this difficult hurdle, demonstrating the highest level of dedication and professionalism and accomplishing what no other class has done before. With your education and skillset from the College of Engineering, you are uniquely qualified to take on what comes next. You will enter an engineering workforce …

Evolutionary Selection Against Short Nucleotide Sequences In Viruses And Their Related Hosts, Yoram Zarai, Zohar Zafrir, Bunpote Siridechadilok, Amporn Suphatrakul, Modi Roopin, Justin G. Julander, Tamir Tuller

Animal, Dairy, and Veterinary Science Faculty Publications

Viruses are under constant evolutionary pressure to effectively interact with the host intracellular factors, while evading its immune system. Understanding how viruses co-evolve with their hosts is a fundamental topic in molecular evolution and may also aid in developing novel viral based applications such as vaccines, oncologic therapies, and anti-bacterial treatments. Here, based on a novel statistical framework and a large-scale genomic analysis of 2,625 viruses from all classes infecting 439 host organisms from all kingdoms of life, we identify short nucleotide sequences that are under-represented in the coding regions of viruses and their hosts. These sequences cannot be explained …

Acute Mechanical Stress In Primary Porcine Rpe Cells Induces Angiogenic Factor Expression And In Vitro Angiogenesis, Farhad Farjood, Amir Ahmadpour, Sassan Ostvar, Elizabeth Vargis

Biological Engineering Faculty Publications

Background

Choroidal neovascularization (CNV) is a major cause of blindness in patients with age-related macular degeneration. CNV is characterized by new blood vessel growth and subretinal fluid accumulation, which results in mechanical pressure on retinal pigment epithelial (RPE) cells. The overexpression of RPE-derived angiogenic factors plays an important role in inducing CNV. In this work, we investigated the effect of mechanical stress on the expression of angiogenic factors in porcine RPE cells and determined the impact of conditioned medium on in-vitro angiogenesis.

Results

The goal of this study was to determine whether low levels of acute mechanical stress during early …

Co Sense And Release Flavonols: Progress Toward The Development Of An Analyte Replacement Photocorm For Use In Living Cells, Marina Popova, Tatiana Soboleva, Abby D. Benninghoff, Lisa M. Berreau

Chemistry and Biochemistry Faculty Publications

Carbon monoxide (CO) is a signaling molecule in humans. Prior research suggests that therapeutic levels of CO can have beneficial effects in treating a variety of physiological and pathological conditions. To facilitate understanding of the role of CO in biology, molecules that enable fluorescence detection of CO in living systems have emerged as an important class of chemical tools. A key unmet challenge in this field is the development of fluorescent analyte replacement probes that replenish the CO that is consumed during detection. Herein, we report the first examples of CO sense and release molecules that involve combining a common …

Biological Engineering Student Receives Peak Prize At Usu Robins Awards, Usu College Of Engineering

College of Engineering News

April 23, 2020 — Utah State University biological engineering undergraduate Morgan Bishop received the Undergraduate Researcher of the Year at this year’s Robins Awards. The Peak Prize is awarded to one student each year who shows consistent engagement in independent inquiry through student employment and their independent research grants. Award winners also typically seek out opportunities to share their results in undergraduate research events and professional conferences. She received the award April 10.

Profiling Of Small Non-Coding Rna In Bovine Somatic Cell Nuclear Transfer Embryos During Early Development Through The Maternal-To-Embryonic Transition, Abby D. Benninghoff, Jocelyn Cuthbert

Browse all Datasets

Supplementary File 1. Read lengths and annotation summaries of sncRNA: Microsoft Excel document with information from small RNA sequencing on the aligned, annotated reads.

Supplementary File 2. Normalized and variance-stabilized values for annotated sncRNAs: Microsoft Excel document with normalized and variance-stabilized reads generated by RNA sequencing of small non-coding RNA from cattle embryos.

Supplementary File 3. Results of DESeq2 analyses for miRNAs, tRNA fragments and piRNAs: Microsoft Excel document with results of DESeq2 differential expression analysis comparing scNT vs IVF embryos at each developmental stage and sequential comparisons of developmental stage for both IVF and scNT embryos.

Supplementary File 4. …

Genotyping-By-Sequencing And Ecological Niche Modeling Illuminate Phylogeography, Admixture, And Pleistocene Range Dynamics In Quaking Aspen (Populus Tremuloides), Justin C. Bagley, Neander M. Heming, Eliécer E. Gutiérrez, Upendra K. Devisetty, Karen E. Mock, Andrew J. Eckert, Steven H. Strauss

Ecology Center Publications

Populus tremuloides is the widest‐ranging tree species in North America and an ecologically important component of mesic forest ecosystems displaced by the Pleistocene glaciations. Using phylogeographic analyses of genome‐wide SNPs (34,796 SNPs, 183 individuals) and ecological niche modeling, we inferred population structure, ploidy levels, admixture, and Pleistocene range dynamics of P. tremuloides, and tested several historical biogeographical hypotheses. We found three genetic lineages located mainly in coastal–Cascades (cluster 1), east‐slope Cascades–Sierra Nevadas–Northern Rockies (cluster 2), and U.S. Rocky Mountains through southern Canadian (cluster 3) regions of the P. tremuloides range, with tree graph relationships of the form ((cluster 1, …

Mapping Transcriptional Changes Associated With The Maternal-To-Embryonic Transition In Bovine Embryos To Small Non-Coding Rna Profiles In Both In Vitro-Fertilized And Scnt Cattle Embryos, Abby D. Benninghoff, Jocelyn Cuthbert

Browse all Datasets

Supplementary File 1. Normalized reads for annotated mRNA transcripts. Microsoft Excel document providing normalized sequencing reads per sample.

Supplementary File 2. Results of DESeq2 analyses for annotated mRNAs. Microsoft Excel document with results of DESeq2 differential expression analysis comparing scNT vs IVF embryos at each developmental stage and sequential comparisons of developmental stage for both IVF and scNT embryos.

Supplementary File 3. Results of Metascape ontology analyses for differentially expressed mRNAs. Microsoft Excel document with results of Metascape ontology analyses for biological processes associated with predicted mRNA targets of differentially expressed miRNAs.

Supplementary File 4. Cytoscape networks for differentially expressed …

Whose Data Is It Anyway? Lessons In Data Management And Sharing From Resurrecting And Repurposing Lidar Data For Archaeology Research In Honduras, Juan C. Fernandez-Diaz, Anna S. Cohen

Sociology, Social Work and Anthropology Faculty Publications

As a response to Hurricane Mitch and the resulting widespread loss of life and destruction of Honduran infrastructure in 1998, the United States Geological Survey (USGS) conducted the first wide-area airborne lidar topographic mapping project in Central America. The survey was executed by the Bureau of Economic Geology at the University of Texas at Austin (BEG) in 2000, and it was intended to cover 240 square kilometers distributed among 15 flood-prone communities throughout Honduras. The original data processing produced basic digital elevation models at 1.5-meter grid spacing which were used as inputs for hydrological modeling. The USGS published the results …

Caterpillars On A Phytochemical Landscape: The Case Of Alfalfa And The Melissa Blue Butterfly, Matthew L. Forister, Su'ad A. Yoon, Casey S. Philbin, Craig D. Dodson, Bret Hart, Joshua G. Harrison, Oren Shelef, James A. Fordyce, Zachary H. Marion, Chris C. Nice, Lora A. Richards, C. Alex Buerkle, Zach Gompert

Biology Faculty Publications

Modern metabolomic approaches that generate more comprehensive phytochemical profiles than were previously available are providing new opportunities for understanding plant‐animal interactions. Specifically, we can characterize the phytochemical landscape by asking how a larger number of individual compounds affect herbivores and how compounds covary among plants. Here we use the recent colonization of alfalfa (Medicago sativa) by the Melissa blue butterfly (Lycaeides melissa) to investigate the effects of indivdiual compounds and suites of covarying phytochemicals on caterpillar performance. We find that survival, development time, and adult weight are all associated with variation in nutrition and toxicity, including …

Go With Your Gut: The Role Of Gut Microbiota In Health, Lydia Bangerter, Mateja R. Savoie-Roskos, Jeffrey Chapman, Abbigail Sundell, Carrie Durward

All Current Publications

Living within your gastrointestinal tract (gut) are about 100 trillion bacterial cells—your gastrointestinal microbiota—a host of bacteria that play a vital role in your body (Ghaisas, Maher, & Kanthasamy, 2016). Your gut microbiome is responsible for a wide variety of functions that contribute to overall health and may be linked to many chronic diseases including cancer, obesity, and cardiovascular disease (Ghaisaset al., 2016; Peregrin, 2013). This fact sheet will discuss the role the gut microbiome plays in various aspects of health and how to improve your gut health.

Modeling The Alkaline Hydrolysis Of Diaryl Sulfate Diesters: A Mechanistic Study, Klaudia Szeler, Nicholas H. Williams, Alvan C. Hengge, Shina C. Kamerlin

Chemistry and Biochemistry Faculty Publications

Phosphate and sulfate esters have important roles in regulating cellular processes. However, while there has been substantial experimental and computational investigation of the mechanisms and the transition states involved in phosphate ester hydrolysis, there is far less work on sulfate ester hydrolysis. Here, we report a detailed computational study of the alkaline hydrolysis of diaryl sulfate diesters, using different DFT functionals as well as mixed implicit/explicit solvation with varying numbers of explicit water molecules. We consider the impact of the computational model on computed linear free-energy relationships (LFER) and the nature of the transition states (TS) involved. We obtain good …

A New Sampler For The Collection And Retrieval Of Dry Dust Deposition, Janice Brahney, Gregory Wetherbee, Graham A. Sexstone, C. Youngbull, P. Strong, Ruth C. Heindel

Watershed Sciences Faculty Publications

Atmospheric dust can influence biogeochemical cycles, accelerate snowmelt, and affect air, water quality, and human health. Yet, the bulk of atmospherically transported material remains poorly quantified in terms of total mass fluxes and composition. This lack of information stems in part from the challenges associated with measuring dust deposition. Here we report on the design and efficacy of a new dry deposition sampler (Dry Deposition Sampling Unit (DSU)) and method that quantifies the gravitational flux of dust particles. The sampler can be used alone or within existing networks such as those employed by the National Atmospheric Deposition Program (NADP). Because …

Ethics In Archaeological Lidar, Anna S. Cohen, Sarah Klassen, Damian Evans

Sociology, Social Work and Anthropology Faculty Publications

Airborne laser scanning or lidar has now been used by archaeologists for twenty years, with many of the first applications relying on data acquired by public agencies seeking to establish baseline elevation maps, mainly in Europe and North America. More recently, several wide-area acquisitions have been designed and commissioned by archaeologists, the most extensive of which cover tropical forest environments in the Americas and Southeast Asia. In these regions, the ability of lidar to map microtopographic relief and reveal anthropogenic traces on the Earth’s surface, even beneath dense vegetation, has been welcomed by many as a transformational breakthrough in our …

Dynamics Of Larkspur (Delphinium Barbeyi) Pellet Consumption And Tolerance Of The Inhibitory Effects Of Larkspur Alkaloids On Muscle Function In Cattle., Benedict T. Green, Jim Pfister, Dale Gardner, Kevin Welch, Daniel Cook

Poisonous Plant Research (PPR)

Larkspurs (Delphinium spp.) are toxic native plants on foothill and mountain rangelands in western North America, which poison cattle grazing on those rangelands. The purpose of this study was to examine in a laboratory setting, the subclinical effects of larkspur intake and toxicosis, by allowing larkspur susceptible and resistant cattle to self-select the amount of larkspur consumed in pellet form. We hypothesized that there would be differences in short term (9 – 11 day) pellet consumption between susceptible and resistant animals. Two trials were completed, each with larkspur resistant and susceptible Angus steers, and larkspur-alfalfa pellets at a 12% …

Biological Engineering Professor Named Fellow Of National Organization, Usu College Of Engineering

College of Engineering News

April 15, 2020 — Utah State University Professor Ron Sims was named fellow of the Institute of Biological Engineering, or IBE, on April 1.

The Impact Of The Illness Label ‘Gout’ On Illness And Treatment Perceptions In Māori (Indigenous New Zealanders), Nicola Dalbeth, Meihana Douglas, Kate Mackrill, Leanne Te Karu, Maria Kleinstäuber, Keith J. Petrie

Psychology Faculty Publications

Background: Despite contemporary advances in understanding pathogenesis and effective management of gout, beliefs about the disease continue to be focused on gout as a self-inflicted illness. The illness label itself may contribute to inaccurate perceptions of the disease and its management. In Aotearoa/New Zealand, Māori (Indigenous New Zealanders) have high prevalence of severe gout. The aim of this study was to examine the impact of the illness label ‘gout’ on perceptions of the disease and its management for Māori. Methods: Māori supermarket shoppers (n = 172) in rural and urban locations were recruited into a study examining the perceptions about …

Faculty Senate Executive Committee Agenda, April 13, 2020, Utah State University

Faculty Senate & Faculty Senate Executive Committee

Call to Order

• Approval of Minutes - March 16, 2020

University Business

Information

• EPC Monthly Report - April 2, 2020

Reports

• Committee on Committees Annual Report
• Calendar Committee Annual Report
• Recruitment & Retention Annual Report

Old Business

• 404 Student Involvement in Faculty Searches

New Business

• Faculty Senate President Elect

Usu Engineering Programs Ranked In Top 50, Top 100 Nationally, Usu College Of Engineering

College of Engineering News

April 13, 2020 — Three of Utah State University’s College of Engineering graduate programs were again ranked alongside some of the top-performing schools in the nation.

Utah's Own Habitat Guidelines For Sage-Grouse, David K. Dahlgren, Michel Kohl, Eric Thacker, S. Nicole Frey, Terry Messmer

All Current Publications

Those living and working within western North America’s sagebrush landscapes have most likely been exposed to sage-grouse issues for many years now and may have felt anxiety concerning the potential impacts that could result from implementing conservation measures designed to help this iconic species persist. Society’s challenge to seek the delicate balance between the conservation of nature and the growth related to human endeavors exemplifies the need to use the best available science as we move forward. Representative information, especially at the landscape level related to sage-grouse, has been hard to come by. However, due to the vast amount of …

Western Cherry Fruit Fly In Utah Orchards, Diane G. Alston, Marion Murray

All Current Publications

This fact sheet describes the western cherry fruit fly, including hosts, life history, timing control, host injury, and management.

Assessing & Protecting Dark Night Skies In El Morro National Monument, Leslie Kobinsky

Student Research Symposium

Light pollution is causing the disappearance of dark night skies around the world. In the United States alone, 1/3 of people are unable to see the Milky Way where they live (Ramlagan, 2016). National Park Service sites contain some of the darkest skies in the country. Here at El Morro National Monument, these dark skies are a beautiful and healthy benefit to people in the local community and visitors traveling from afar. El Morroês current park legislation does not include specific measures of protection for the night sky. This capstone project will create a baseline data set of night sky …

Construction Of Candidate Vectors For Correction Of The Intestinal Cftr Gene Expression In Cystic Fibrosis Sheep Fetal Fibroblast Cells, Kaden Bunch

Student Research Symposium

Cystic Fibrosis (CF) is a recessive human genetic disease that is caused by mutations in the Cystic Fibrosis Transmembrane Conductance Regulator (CFTR) gene. This gene is responsible to transport Cl- and HCO3- anions in epithelial cells. Previously, we generated CFTR-/- lambs using CRISPR/Cas9 and SCNT techniques. The CFTR-/- lambs display many features similar to human CF disease, including meconium ileus (MI), pancreatic fibrosis, portal fibrosis and biliary hyperplasia, small gallbladder, and absence of vas deferens. In CF patients, MI affects only 15-20% of human babies, whereas it was observed in 100% of newborn CFTR-/- lambs and was the primary cause …

An Evaluation Of The Susceptibility Of Goats To Larkspur Toxicosis, Kevin D. Welch, Clint A. Stonecipher, Dale R. Gardner, Benedict T. Green, Daniel Cook

Poisonous Plant Research (PPR)

Larkspurs (Delphinium spp.) are a major cause of cattle losses in western North America, whereas sheep have been shown to be resistant to larkspur toxicosis. Goats are often used as a small ruminant model to study poisonous plants, even though they can be more resistant to some poisonous plants. It is not known how susceptible goats are to the adverse effects of larkspurs. In this study, we evaluated the susceptibility of goats to larkspur toxicosis by performing a dose-response study. We dosed goats with D. barbeyi collected near Cedar City, Utah at 3.3, 4.4, 6.6, 8.8 and 10.0 g …

Soil Microbe Identification: An Innovative Approach To Teaching Microbiology Labs, Autumn Kelsch

Student Research Symposium

In the lab I plan to develop, students will collect soil from a location of their choice and try to isolate and identify bacteria from the samples. Working with bacteria from soil instead of from an individual's body (which is the approach currently being used in the course) will remove the threat of spreading a communicable disease and will allow students to work in groups. This will cut down the cost, but more importantly it will strengthen the students' teamwork skills - a major objective of the class. Students will then be responsible for keeping their cultures growing for several …

Biomethanation Of Invasive Water Hyacinth From Eutrophic Waters As A Post Weed Management Practice In The Dominican Republic: A Developing Country, Yessica Castro

Student Research Symposium

Anaerobic digestion of water hyacinth (Pontederia crassipes Mart.) from eutrophic water bodies could be a sustainable post weed management practice to generate bioenergy. Comparative analyses of the water quality, physicochemical characteristics, and biomethanation kinetics of water hyacinth from two sites with different water types (brackish versus freshwater) in the Ozama river, Dominican Republic, were conducted. Also, the energy produced from the anaerobic digestion and that consumed in harvesting was estimated. The highest non-structural components in the form of protein (18.8 ± 1.9%) and extractives (26.4 ± 0.1%) were found in brackish water hyacinth, whereas that from freshwater had the highest …