Digital Commons Network^™

Using Cytoplasmic Biopsies To Determine Bovine Oocyte Quality, Madison Lindsey

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Embryos resulting from assisted reproductive technologies, such as in vitro fertilization (IVF) or intracytoplasmic sperm injection (ICSI), develop with lower efficiencies than embryos resulting from their in vivo counterparts. The reasons behind the developmental discrepancies remain largely unknown. Because the egg is the primary determinant of embryo developmental success, it is reasonable to consider inherent egg quality as a possible cause. The hypothesis for this project is that there are distinct mRNA transcript patterns, or molecular “fingerprints,” that distinguish high- versus low-quality eggs developing within the same environment. In this study, a small cytoplasmic biopsy was removed from 40 eggs …

Investigating Cause-Specific Mortality Of Sheep To Determine The Impacts Of Carnivores On Domestic Livestock, Nathan Jacob Floyd

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Livestock and carnivores interact in ways that are considered conflict throughout the world. In the western United States, livestock are often grazed on public lands in close proximity to predators in their natural habitat, and can be killed as prey. Livestock losses to predators can threaten rancher’s livelihoods. Sheep and lambs are especially vulnerable to predators due to their small size and lack of defensive abilities. To reduce the impacts that predators have on livestock, it is important for ranchers and wildlife biologists to have an accurate understanding of how many livestock die and are killed by predators when grazing …

High-Isolation Antenna Technique For Cubesat-Borne, Continuous-Waveform Radar, Logan E. Voigt

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Radar is important in target tracking, imaging, and weather prediction applications. As technology is increasingly miniaturized, there is a push for smaller radar. Research and exploration in outer space also benefit from small, low-power technologies. The NASA Jet Propulsion Laboratory’s RainCube was the first successful CubeSat-borne radar. A CubeSat is a type of small satellite that conforms to specific size and weight standards. Radar technology benefits from additional research on how to further miniaturize radar payloads.

Integrating the transmit and receive antennas on the solar panels removes the need for antenna-deployment mechanisms, preserving space on the CubeSat. This thesis also …

The Personal Is Still Political: An Analysis Of Jessica Valenti's Contemporary Feminine Style, Michala M. Zilkey

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

In 2016, Jessica Valenti’s memoir, Sex Object, was published. Sex Object describes Valenti’s experiences of sexualization and objectification in a personal and anecdotal format. The feminine style, a rhetorical style that bridges the divide between norms of femininity and expectations of public oration, has been heralded as essential for advancing the feminist movement. This thesis asks the question, to what extent does contemporary feminists’ rhetoric mirror and diverge from earlier iterations of the feminine style? I argue that Valenti’s employs a feminine style that utilizes relational norms and self-disclosure to illustrate the personal as political and the political as …

Urban Soil Chemical And Nutrient Management Issues Facing Emerging Small Grower Enterprises In Utah, Frank E. Oliver

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Knowledge of both soil quality and contamination has become increasingly important with the growth of urban agriculture in Utah and the United States as whole. Land is also a common limiting factor in urban agriculture, so it is important to maximize yield and net returns. In order to meet these demands, three studies were conducted across key urban agriculture sites along the Wasatch Front: 1) an urban soil survey to assess soil contamination, 2) an urban soil survey to evaluate macronutrient and salinity levels, and 3) a nitrogen fertilizer management trial for dahlia (Dahlia pinnata), a cut flower …

Institutional Betrayal Related To Sexual Trauma In Military Service Members And Veterans: An Examination Of Posttraumatic Sequelae, Felicia Andresen

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Military sexual harassment and/or assault (MSH/A) is pervasive and destructive. MSH/A survivors who believe the institution played a role in the traumatic experience by failing to respond in a supportive manner or for failing to keep them safe may feel betrayed by the military itself, a concept referred to as institutional betrayal. Previous research suggests it is more harmful to be abused by trusted others or institutions due to the violation of trust. This two-study dissertation explored experiences of MSH/A-related institutional betrayal to identify survivors at-risk for worse posttraumatic outcomes.

Study #1 evaluated whether PTSD explained the relationship between …

Teacher Home Visits: Changes In Perceptions Of Key Stakeholders, Kevin R. Thomas

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Teacher home visits have been shown to improve student attendance, academics and to reduce behavior issues. Three groups are involved: the student, the teacher, and the family (or parent). This research focused on determining if and how the teacher home visit changes the perspectives, expectations, and relationships of these three groups. A survey followed by interviews of some of the students, parents, and teachers was used. The surveys and the interviews were evaluated. Results indicated that perceptions and relationships between stakeholders were improved. Due to low participation rates, results must be interpreted with caution. With these considerations in mind, I …

Bugs Buy Steady Releases From Hydropower Producers To Encourage More Synergistic Reservoir Management, Moazzam Ali Rind

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Hydropower generated from dams has significant economic value, however, that value is achieved at the cost of native ecosystem devastation. Here, we have estimated loss in hydropower revenue due to inclusion of the steady low flow days –Bug Flow Experiments. We developed a linear optimization model and constraint method that restrict the number of steady low flow days while maximizes the hydropower revenue generation. The results suggested that increase in release volume will benefit both the objectives (win-win scenario), energy price differential between on-and off-peak periods controls the position and shape of tradeoff curves, and offset release does not have …

Evaluating Microbial Safety Of Food Products And Processes In Small Scale Food Services, Processors And Manufacturers, Sujan Acharya

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

This study evaluated microbial safety of relatively new and modified food products prepared especially by small-scale foodservice establishments and home producers. In the first part, microbial safety during fermentation at room temperature was evaluated. Chapter 3 monitored Listeria monocytogenes and Escherichia coli O157 survival during Sauerkraut’s natural fermentation. Chapter 4 evaluated the survival of acid-adapted E.coli O157 during kombucha fermentation and in kombucha-fruit blend during refrigeration. During both fermentations, the pathogens were inactivated before the recommended duration of fermentation. However, E.coli O157 introduced after fermentation survived in kombucha-fruit blends during refrigerated storage. The second part (Chapter 5) evaluated the safety …

A Social-Ecological Approach To Understanding The Structure, Function, And Challenges Faced By Fisheries, Chase C. Lamborn

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Fisheries throughout the world play many important roles, and they are also subject to many pressures. The tension between roles and pressures is increasing, and as a result, many fisheries are being utilized to, or over their capacity, which ultimately threatens their sustainability. To address these challenges, this dissertation provides insights and tools to help us better understand fisheries and the challenges they face. The first study explores the impacts of an earlier spring runoff in Montana. Earlier runoff has resulted in lower and warmer summer flows, which is stressing coldwater species like trout. This stress is affecting fishing quality, …

Emotional Support Animal Partnerships: A Multimethod Investigation, Jillian Ferrell

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Emotional Support Animals (ESAs) are increasing in prevalence and awareness, though not all the publicity and attention have been positive. Many people, including housing officials, persons with disabilities, health professionals, and the general public are confused about the roles and rights of ESAs. Misunderstandings, lack of awareness, and fraud have led to dangerous and inappropriate situations for humans and animals alike despite overwhelming evidence of powerful therapeutic benefits of the human animal bond.

Three studies provide insight into various perspectives involved in ESA partnership experiences and development. Each study provides a theoretical, quantitative, and qualitative approach, respectively, to explore the …

Conservation Conflict: Rare Plant Conservation And Energy Development And Potential In The Colorado Plateau, Joshua Carrell

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

The Colorado Plateau provides numerous sources of energy, particularly natural oil and gas. This energy potential is being actively extracted for consumption on both public and private lands. Elements of biodiversity (e.g., listed and sensitive plant and animal species) are distributed among all land tenures, yet the laws protecting them can vary depending if they occur on public, private, or tribal land. Therefore, it is necessary to understand the geographic ranges of threatened, endangered, and sensitive species and their relationship to land ownership to preserve habitat and conserve species populations. I developed species distribution models (SDMs) to examine the predicted …

Spatial-Temporal Responses Of Cow Elk To Targeted And Non-Targeted Hunting Risk, Randall Mcbride

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Predation is one of the most important interspecies interactions that affect a wildlife population. Predator-prey interactions may cause species to shift their behavior, such as changing the use of their habitat in both space and time, based on their ability to assess risk. Wildlife population responses to predation stresses have been studied in the past, but individuals' responses to these stresses have not been studied in fine detail. For this study, cow elk were collared with GPS collars, and hunters carried hand-held GPS units. GPS locations were taken at 10-minute intervals for both cow elk and hunters during the hunting …

Examining The Effectiveness Of Explicit, Systematic Mathematics Vocabulary Instruction For Students With Learning Difficulties And Disabilities In A Specialized Setting, Kristen R. Rolf

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Many students in the United States are not proficient in mathematics. Mathematics vocabulary is one area that may impact students’ understanding of and engagement with mathematics. This dissertation investigated the implementation, effectiveness, and teacher’s perceptions of a program for teaching mathematics vocabulary necessary for fourth grade and beyond.

This study randomly assigned 30 students (11-14 years old) to receive mathematics vocabulary instruction or not. Three teachers at a school for students with learning difficulties and disabilities administered mathematics vocabulary and mathematics achievement tests to all of their students before teaching the program to 17 of the students. A research assistant …

Modeling A Pandemic: Investigating Student Learning About Disease Spread In The Context Of Agent-Based Modeling, Siyu Wu

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

The COVID-19 pandemic has highlighted a need for students to learn about public health issues, including the transmission of disease and methods for the prevention of epidemics. This study presents data from a project focused on developing computational microworlds to help middle school students learn about these topics. The microworld is designed to help students model and test their ideas about how a disease spreads through a population and how an epidemic can be prevented. I employed a lab-based case study approach to conduct one-on-one 1.5-hour interviews through Zoom with four middle-school students (ages 12-14). During the interview, the student …

Natural History And Development Of Melittobia Acasta On Megachile Rotunda , Alan R. Anderson

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Alfalfa is one of the most economically important crops in North America. To produce alfalfa seed, a pollinator must release plant reproductive organs by applying pressure to keel petals. The alfalfa leafcutting bee, Megachile rotundata, is the primary commercial pollinator of alfalfa. U.S. bee managers struggle to maintain populations due to many mortality factors. One cause is attack from parasitoid wasps such as Melittobia acasta. Females can lay hundreds of eggs and have multiple generations, which can decimate bee stocks. Chapter Two investigates the life cycle and base temperature that allows development of M. acasta on bee hosts. …

Impacts Of The Changing Pacific On North American Drought, Atmospheric Rivers, And Explosive Cyclones, Jacob Stuivenvolt-Allen

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

The impacts of specific weather events can vary greatly from year to year. Much of these impacts depend heavily on the frequency of impactful weather which is constrained by the state of the climate system each year. This research focuses largely on the impacts that climate oscillations from year-to-year or even from decade-to-decade have on the frequency of impactful weather. There are numerous examples of impactful weather that impact North America, but this work focuses on drought in the western United States, atmospheric rivers in Northern California and rapidly developing winter storms along the east coast. While seemingly disparate events, …

High School Biology Teachers' Integration Of Argumention In The Context Of Disciplinary Literacy Coaching, Ashley Strong

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Scientific argumentation is a core scientific practice. Although scientific argumentation has been linked to increased learning of scientific content, improved reading, writing, and critical thinking, traditional science classrooms have not included scientific argumentation. Teachers often have little experience learning or teaching science through argumentation and need support to integrate this complex practice of science into their classrooms. This study compared four high school biology teachers’ instruction of scientific argumentation as they worked with a literacy coach. Data were collected through interviews, audio recordings of classroom instruction and literacy coaching sessions, and artifacts. After analyzing the data, several key findings stood …

Maternal Effects And Management Of Alfalfa Leafcutting Bees, Makenna May Johnson

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Most bees are solitary and important contributors to pollination of a variety of crops. Solitary bees do not live in colonies. Instead, females work individually to construct nests and provision nutrients for each offspring. However, solitary bees can be difficult to manage, especially on a large scale like honey bees, because of differences in how species provision for their offspring and construct nests. Variations in the timing of offspring development to adulthood even within species makes commercial management difficult. Variations in reproductive strategy, like how many offspring to make, how much provision to give each, and when to make them …

Evaluation Of Noninvasive Methods For Determining Pregnancy, Diet, Nutrition, And Stress Among Pronghorn Antelope: Implications For Population Monitoring, Cole Bleke

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

This study was conducted to increase our understanding of the influence of adult female pronghorn (Antilocapra americana) physiology on fawn summer survival. Pronghorn are a valued big game animal in Idaho, but are particularly sensitive to capture; therefore, we designed a noninvasive fecal sampling study to measure pregnancy (progesterone and estrogen), diet, nutrition (fecal nitrogen and DAPA), and stress (cortisol), during metabolically-demanding female life history stages (late gestation, early lactation, and breeding season). We also compared survey results between ground and aerial methods within mountain valley landscapes.

Pregnancy determination, via progesterone, is possible for pronghorn, but sample collection …

Hidden Mechanisms Of Climate Impacts In Western Forests: Integrating Theory And Observation For Climate Adaptation, Sara J. Germain

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Fire, insects, and disease are necessary components of forest ecosystems. Yet, climate change is intensifying these tree stressors and creating new interactions that threaten forest survival. This dissertation combined field observations with statistical predictions of changing disturbances in western forests to identify 1) how conventional models may underestimate future forest loss, and 2) how positive relationships between trees may be exploited by managers to prevent forest loss.

In Chapter II, I tested whether increasingly extreme weather with climate change increases Pacific yew extinction risk. I found that conventional modeling methods underestimated local extinction risk because trees were adapted to a …

Psychosocial Development In Adolescents With Disabilities: Modification And Evaluation Of Measures, Myles Maxey

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Adolescents with intellectual and developmental disabilities (IDD) are often overlooked in research, including psychosocial development (self-esteem, autonomy, attachment, and identity formation). Reliable and consistent survey tools are essential to understanding developmental trends. This study focused on creating surveys that could be comprehended by all adolescents, no matter if they experience IDD or not. Findings from this study suggested that there are more similarities than difference across areas of psychosocial development among adolescents with IDD as well as with their typically developing peers. These findings can help researchers more accurately assess psychosocial development for more adolescents, including those experiencing IDD.

Implications Of Malthus-Boserup Ratcheting For Interpreting The Archaeological Record, Gideon F. Maughan

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Prehistoric populations across North America seem to grow exponentially, with some variation between regions. Archaeologists have explored the differences somewhat, but have not explained the differences or the sustained growth with any reference to what may be going on under the surface in a way that is relevant to all regions. I propose that environmental limits on population are shaped by what populations eat and how they acquire food, and that when populations are large enough to feel the scarcity in their environment, they change their way of life in a way that increases those limits. The model I propose …

Masculinity, Affirmations, Belongingness, And Resiliency In Male Adolescents: Effects On School Engagement, Kelsey Burt

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

This study focused on male adolescent students within 7^th and 8^th grade and how endorsement of masculine stereotypes impacts their school engagement and feelings of belonging. The study also examines how peers maintain masculine conformity and how individual resiliency change outcomes. This research is based on two main theories, inclusive masculinity and social learning theory. These theories describe how behaviors are largely learned from the social environment and maintained due to reinforcement or punishment. There were 127 adolescent males who participated in this study and were recruited through an online survey system. Results of the study show that …

Changes In Healthy Eating And Physical Activity Behaviors Of Adult Participants In Create Better Health's Education (Snap-Ed) Program, Kami Bullock

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

The purpose of this study was to compare the effects of delivery method on the nutrition, food resource management (FRM), and physical activity behavior of participants enrolled in either Create Better Health’s in-person or online courses. The pre-survey and post-survey included data on nutrition, FRM, and physical activity behaviors. The researcher analyzed preexisting data from 138 SNAP-eligible adults who participated in Create Better Health’s online and in-person courses.

Results showed similar improvements in most of the nutrition and physical activity behaviors among those who took the CBH courses online or in-person. The online course showed statistically higher scores for four …

Upstream Methods For Enhancing Engineered Curcumin Biosynthesis, Caleb D. Barton

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Curcumin is a bright orange compound with myriad applications for human health and wellness. Curcumin occurs naturally in the plant Curcuma longa (commonly known as turmeric) but must be extracted from the roots in an environmentally unfriendly fashion to obtain commercially relevant amounts of the compound. In addition, extraction of curcumin from turmeric spice yields a mixture of various curcuminoids, presenting an issue for isolating it in its pure form and complicating its use in clinical settings.

Heterologous biosynthetic production of curcumin in Escherichia coli has been used extensively as a viable alternative to plant extraction but suffers from poor …

Comparing Fecal Dna Capture-Recapture Methods To Traditional Mark-Resight Methods For Estimating Abundance Of Mule Deer On Winter Ranges, Andi M. Stewart

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Well-designed monitoring strategies are required to obtain accurate estimates of population abundance, which is important for evaluating conservation and management strategies. Obtaining abundance estimates using traditional survey methods, like aerial surveys, is not possible in all scenarios and can be expensive and risky. One survey method that has gained popularity in the last decade is fecal DNA-based capture-recapture (CMR). However, this method has not been evaluated on winter ranges or in snowy, winter conditions.

My first objective was to implement fecal DNA CMR to estimate abundance of mule deer in the Round Valley and Goodale winter survey areas in the …

Lentiviral Vector Production At High Cell Density By Transient Transfection Of Suspended Culture Hek Cells, Jacob G. Accordino

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Viral vectors are gene carriers that efficiently deliver therapeutic gene constructs to target cells. Viral vectors are frequently produced in suspension-growing cells of mammalian origin, referred to as packaging cells. Lentiviral vectors have become widely used as gene vectors since they were initially developed two and a half decades ago, particularly in research settings. The advantages of lentiviral vectors for treating diseases of genetic origin have driven research into their large-scale manufacture for clinical settings. Currently, three main challenges exist that limit mass production of lentiviral vectors. First, plasmid DNA used in the production process is very expensive. Second, high …

Limited Effects Of Precipitation Manipulation On Soil Respiration And Inorganic N Concentrations Across Soil Drainage Classes In Northern Minnesota Aspen Forests, Anna B. Stockstad, Robert A. Slesak, Alan J. Toczydlowski, Charles R. Blinn, Randall K. Kolka, Stephen D. Sebestyen

Aspen Bibliography

It is critical to gain insight into the responses of forest soils to the changing climate. We simulated future climate conditions with growing season throughfall reduction (by 50%) and winter snow removal using a paired-plot design across a soil drainage class gradient at three upland, Populus-dominated forests in northern Minnesota, USA. In situ bulk soil respiration and concentrations of extractable soil N were measured during the summers of 2020–2021. Soil respiration and N concentrations were not affected by throughfall reduction and snow removal, which was largely attributed to the limited treatment effects on soil moisture content and soil temperature. …

Coping With Environmental Constraints: Geographically Divergent Adaptive Evolution And Germination Plasticity In The Transcontinental Populus Tremuloides, Roos Goessen, Nathalie Isabel, Christian Wehenkel, Nathalie Pavy, Lisa Tischenko, Lyne Touchette, Isabelle Giguère, Marie-Claude Gros-Louis, Jérôme Laroche, Brian Boyle, Raju Soolanayakanahally, Karen E. Mock, Javier Hernández-Velasco, Sergio Leonel Simental-Rodriguez, Jean Bousquet, Ilga M. Porth

Aspen Bibliography

Societal Impact Statement

Syntheses clearly show that global warming is affecting ecosystems and biodiversity around the world. New methods and measures are needed to predict the climate resilience of plant species critical to ecosystem stability, to improve ecological management and to support habitat restoration and human well-being. Widespread keystone species such as aspen are important targets in the study of resilience to future climate conditions because they play a crucial role in maintaining various ecosystem functions and may contain genetic material with untapped adaptive potential. Here, we present a new framework in support of climate-resilient revegetation based on comprehensively understood …