Digital Commons Network^™

The Cellular Structure Of Selected Apple Varieties, K. G. Lapsley, F. E. Escher, E. Hoehn

Food Structure

Apple cultivars (Sauergrauech, Klarapfel, James Grieve, Granny Smith, Mcintosh, Robinette) which had different textures based on sensory and instrumental analysis (particularly in firmness and mealiness) were examined by conventional scanning electron microscopy (SEM), cold-stage SEM (cryoSEM) and confocal scanning laser microscopy (CSLM) using various preparative procedures. Advantages, lim itations and artifacts of each technique are discussed.

SEM with glutaraldehyde-fixation and criticalpoint- drying produced minimal tissue distortion and the fracture pattern and appearance of mealy versus non mealy tissue was different. Freeze-drying unfixed tissue caused cell collapse and firm versus soft varieties could not be differentiated. Freeze-fracturing and cryoSEM of apple …

Relating Spectral Observations Of The Agricultural Landscape To Crop Yield, Craig L. Wiegand, Arthur J. Richardson

Food Structure

Remote sensing and microscopy share several common concerns including wavelength and sensor selection, signal processing, and image analysis. For crop yield assessments, multispectral observations are acquired photographically, videographically, or with opticalmechanical scanners from aircraft and spacecraft. Sensors are chosen at wavelengths of high atmospheric transmission and maximum contrast between the soil background and the vegetation growing out of it. Vegetation indices have been developed that maximize the information about the photosynthetic size of the vegetation in the landscape and, hence, about crop stresses and yield . Three such indices that reduce the multispectral observations to a single numerical index are …

Identification Of Proteins And Complex Carbohydrates In Some Commercial Low-Fat Dairy Products By Means Of Immunolocalization Techniques, Barbara L. Armbruster, Nitin Desai

Food Structure

Macromolecular assemblies of proteins and hydrocolloids in low-fat dairy products contribute to the structure and texture of these foods. Immunolocalization techniques were used to identify B-lactoglobulin, casein, bovine whey proteins, and egg albumin in low-fat frozen desserts, reduced fat process cheese, and salad dressings. SimpJesse18 I 00 protein particles were examined and characterized by these methods and compared to naturally occurring protein structures in the low-fat foods. Hydrocolloid identification in low-fat salad dressing was accomplished by complexing cellulase and hemicellulase with colloidal gold and applying the probe as a pre-embedding step, on sections of embedded specimens or on material dried …

Distribution Of Aromatic Compounds In Coastal Bermudagrass Cell Walls Using Ultraviolet Absorption Scanning Microspectrophotometry, Nancy P. Ames, Roy D. Hartley, Danny E. Akin

Food Structure

The distribution of aromatic constituents, including lignin , in the leaf cell walls of "Coastal" hermudagrass (Cynodon dacrylon (L.) Pers.) was investigated using scanning ultraviolet (UV) microspectrophotometry. Leaf blade sections and individual tissue types were scanned at three wavelengths representing the absorbance maxima (318, 287 and 250 nm) of aromatic constituents present in bermudagrass leaves. The measured absorbance data were printed in a geometric arrangement to produce an image of the distribution and amount of aromatic constituents among and within cell wall types which vary in digestibility . Differences in absorbance were observed among cell wall types, among walls of …

Effect Of Chemical Modifications On The Stability, Texture And Microstructure Of Cooked Meat Batters, Andre Gordon, Shai Barbut

Food Structure

The effects of five chemical agents on the stability, texture and microstructure of cooked comminuted meat batters were studied. B-mercaptoethanol (/3-ME), hydrogen peroxide (H20~, ethylenediaminetetraacetic acid (EDT A), urea, and polyoxyetbylene sorbitan monooleate (fween 80) were used. The reduction of disulphide bonds by 8-ME or the oxidation of free sulphydryls by H20 2 prior to cooking did not affect liquid and fat losses during cooking as compared to the control (2.5% NaCI). However, texture profile analysis (fPA) showed that treatment with H20 2 resulted in a harder, more cohesive product than the control and ,8-ME treatments because a more fine-stranded, …

Cryo-Scanning Electron Microscopy Investigation Of The Texture Of Cooked Potatoes, J. T. Van Marle, A. C.M. Clerkx, A. Boekestein

Food Structure

The texture of steam-cooked potatoes was investigated by examining the fracture planes of four different cultivars, using cryo-scanning electron microscopy (cryo-SEM), which yielded a good preservation of the hydrated structures in potato tissue . For all cultivars , fracturing after steam -cooking took place between cells preferentially alongside the cell walls. However, textural difference appeared from the degree of intercellular contact, the cell shape and the appearance of cell surfaces. Cells in the fracture planes of firm potatoes had large intercellular contacts. In this case, most of the cells were flat and cell surfaces showed folds and cracks . For …

Physicochemical Properties Of Irradiation Modified Starch Extrudates, A. S. Sokhey, R. Chinnaswamy

Food Structure

Corn starch samples containing 0, 25, 50 and 70% amylose irradiated with -y -radiation dosages of 0 (native) , 5 , 10, 20 (containing 0, 2.5 or 5% potassium persulfate , hydrogen peroxide or eerie ammonium nitrate) or 30 kGy were extrusion cooked using a C. W. Brabender single-screw extruder at a barrel temperature of 140 °C, a screw speed of 140 rpm and 18% moisture content. Increasing irradiation dosages from 0 to 30 kGy gave consistently increased expansion ratios from 2.9 to 7.9 for 25% amylose starches and from 6.5 to 14.5 for 50% amylose starches, while the expansion …

Investigation On The Collagen Fiber Network In Human Dental Tissues - Transmission And Scanning Electron Microscopy, C. Marchetti, P. Menghini, C. Piacentini

Cells and Materials

The collagen fiber component of pulp, dentine and cementum, has been studied by isolating it from the inorganic component and the organic non-collagen matrix. This study was carried out on healthy molar and premolar adult teeth. Following demineralization all of the non-collagenous components of the tooth were removed by sequential treatment with H202, with different concentrations of trypsin and with EDTA. The transmission (TEM) and scanning (SEM) electron microscopy investigation confirmed that such a technique is an accurate method for isolating the collagen fibers while preserving their position and their ultrastructure in the dental tissue. The use of TEM and …

Scanning Electron Microscope Observation Of Lubricious Polymer Surface For Medical Use, Hiroyuki Inoue, Yoshikimi Uyama, Emiko Uchida, Yoshito Ikada

Cells and Materials

A peculiar textured structure was found by scanning electron microscopic (SEM) observation on polymer films surface-grafted with water-soluble polyacrylamide and poly(N,N-dimethylacrylamide) after exposure to ozone. The grafted surface became very hydrophilic and highly lubricated when brought into contact with water, due to hydration of the grafted surface layer. Unless water was present, the surface did not become slippery at all. SEM of the grafted surface revealed different profiles, depending on the graft density as well as the process of drying prior to the SEM observation. There was no significant difference between the virgin and the surface-grafted film, when air-dried under …

Interaction Of Liposomal Drug Delivery Systems With Cells And Tissues: Microscopic Studies, M. Foldvari, G. T. Faulkner, C. Mezei, M. Mezei

Cells and Materials

Liposomes , as drug carriers, can be administered into the body by several routes e.g. intravenously, intraperitoneally, intramuscularly, intratracheally and topically among others. Radiolabelled markers are suitable to monitor the distribution and elimination of liposomes, but the tissue deposition of intact liposomes, the mode and sites of drug release from the liposomes and liposome-cell interactions cannot be investigated morphologically. Microscopic techniques could provide information regarding the intact state of liposomes and possibly the dynamics of liposomes in tissues provided that they can be identified with certainty in vivo. This is a formidable problem and in spite of several attempts, there …

Evaluation Of Bacterial Glycocalyx Preservation And Staining By Ruthenium Red, Ruthenium Red-Lysine And Alcian Blue For Several Methanotroph And Staphylococcal Species, Theresa A. Fassel, James E. Van Over, Christopher C. Hauser, Lorie E. Buchholz, Charles E. Edmiston, James R. Sanger, Charles C. Remsen

Cells and Materials

The cationic reagents, ruthenium red (RR), alcian blue (AB) and ruthenium red -lysine were compared for ultrastructural preservation and staining of the bacterial glycocalyx. En bloc RR, AB and RR-lysine procedures, and a glutaraldehyde/OsO4 fixation withou t cationic reagent, were evaluated by transmission electron microscopy (TEM) for several methanotroph and staphylococcal species. The glutaraldehyde/OsO4 fixation was totally ineffective in preserving or staining the glycocalyx material in all species studied. The RR procedure was more effective than the glutaraldehyde/OsO4 fixation but was generally less so than the RR-lysine procedure. By the RR-lysine procedure, extensive layers that were often filamentous or fibrous …

Utah Science Vol. 53 No. 4, Winter 1992

Utah Science

The Center for Meat Processing Technologies:

106 Trimming the Meeknees from Red Meat Marketing

The Center for Value Added Seed Technology:

113 Taming Apomixis to Create New Wheat Hybrids

119 Research in Brief

124 Better Resistance Against Dwarf Smut

125 Taming Range Grasses to Create Better Turf Grass

126 Removing a Biotech Bottleneck

The Center for Dairy Foods Technology:

128 Helping the Dairy Industry Gear Up for the next Century

1992 Index

133 New Publication

136 1992 Subject Index

Soil Nitrogen Dynamics Following Harvesting And Conversion Of Red Alder And Douglas-Fir Stands, H. Van Miegroet, P. S. Homann, D. W. Cole

Watershed Sciences Faculty Publications

Drastic reductions in NO3- leaching have been observed after harvesting of mature red alder (Alnus rubra Bong.) stands. Our objective was to examine whether these reduction were linked to changes in soil N dynamics. Adjacent alder and Douglas fir [Pseudotsuga menziessii (Mirbel.) Franco] stands on young glacial soils (Alderwood; a loamy-skeletal, mixed, mesic, ortstein Aquic Haplorthod) in western Washington were harvested and replanted with either alder or Douglas fir seedlings; reference plots were established in nearby undisturbed stands. Three years after site conversion, when NO3- leaching declined most drastically in the harvested alder plots, net N mineralization and net nitrification …

Visual Feeding By Juvenile Bear Lake Sculpin, D. Neverman, Wayne A. Wurtsbaugh

Watershed Sciences Faculty Publications

Young-of-the-year Bear Lake sculpin Cottus extensus feed throughout the day on benthic invertebrates and cease feeding at night when they migrate to the metalimnion. We investigated their reliance on vision by conducting feeding trials at different light levels in the laboratory. Feeding rate reached a maximum at intermediate light levels (near 10^16 photons sm^–2 ss^–1; approximately 1 lux) but decreased as light intensity increased beyond this range. At this maximum rate, the fish fed nine times faster than they were able to feed in the dark, showing that young Bear Lake sculpin rely heavily upon vision to feed. The light …

A Trophic Gradient Analysis Of Lake Powell During Spring Runnoff 1992, Wayne A. Wurtsbaugh, Clyde Lay, Darren Carlise, Corey Huxol, Craig Schaugaard, Beau Clements, David Beauchamp

Watershed Sciences Faculty Publications

Between 26 April and 2 May 1992, students in a utah state university aquatic ecology class visited Lake Powell to do a study of trophic gradients of the reservoir. The main axis of the reservoir was surveyed, as well as less detailed analyses of Moki and Escalante Canyons. The work was conducted in collaboration with personnel from the u.s. Bureau of Reclamation headed by Bill Vernieu, and from the u.s. Geological Survey (Dick Marzolf). Some of their data appear in the physical-chemical section of this report.

Each student was responsible for compiling a report on one of the following specific …

Cliff Lake Bench Research Natural Area: Problems Encountered In Monitoring Vegetation Change On Mountain Grasslands, Walter F. Mueggler

Aspen Bibliography

No abstract provided.

Armillaria Root Rot In The Canadian Prairie Provinces, K. I. Mallett

Aspen Bibliography

No abstract provided.

Lake Trout Spawning In Lake Tahoe: Egg Incubation In Deepwater Macrophyte Beds, D. A. Beauchamp, B. C. Allen, R. C. Richards, Wayne A. Wurtsbaugh, C. R. Goldman

Watershed Sciences Faculty Publications

Although most populations of lake trout Salvelinus namaycush spawn over rocky shoals, use of these substrates by lake trout has not yet been found in Lake Tahoe. Large cobble substrate exists at depths less than 20 m, and steep, fractured, rocky substrate can be found in isolated areas from the surface down to at least 100 m, but no evidence of spawning activity in these areas has been found. Instead, at least a portion of the population spawns on deepwater mounds (40–60 m deep) over beds of the macrophyte Chara delicatula. This is the first known report of lake trout …

White Pine Weevil Response To Oak Overstory Girdling - Results From A 16-Year-Old Study, S.A. Katovich, F.S. Morse

Aspen Bibliography

No abstract provided.

Evaluation Of Mechanisms Of Alteration And Humification Of Pahs For Water Quality Management, Ronald C. Sims, Carolyn K. Abbott

Reports

Introduction: Creosote-pentachlorophenol (PCP) is a mixture commonly used as a wood preservative in the U.S. (1). A 1988 survey (2) indicated that 1,397 wood preserving waste contaminated sites exist in the United States consisting of 555 active wood treatment plants and 842 inactive plants. Stinson (3) indentifed 58 wood preserving sites on the National Priorities List, of which 51 have PCP and/or creosote or polycyclic aromatic hydrocarbon (PAH) contamination. Principal classes of organic constituents present in creosote waste are PAHs (~85% by weight) and phenolics. PAHs with less than three fused benzene rings comprise 69% (i.e., naphthalene, anthracene and phenanthrene); …

Bioengineering For Water Cleanup: State-Of-The-Art Assessment, Judith L. Sims, Ronald C. Sims, Jon S. Ginn

Reports

No abstract provided.

Composting Of Explosives Contaminated Soil Using The White Rot Fungus Phanerochaete Chrysosporium, Michael J. Mcfarland, Shashi Kalaskar, Eric Baiden

Reports

Bioaugmentation using the white rot fungus Phanerochaete chrysosporium was found to be effective in enhancing TNT removal in lightly (i.e., below 100 mg/kg) contaminated soil. The percent reduction in TNT increased from 15% to 53% when soils were treated with fungal inoculated organic material. Maximum removal rates improved from 0.33 mg TNT/kg-day to 0.76 mg TNT/kg-day during the same treatment. Neither fungal inoculation nor organic amendment addition resulted in improving TNT removal kinetics in highly contaminated soils (i.e., above 1000 mg/kg). Executive Summary: Laboratory scale compost studies were conducted to evaluate the effect of bioaugmentation with the white rot fungus,

Characterization Of The Ecological Integrity Of Commercially Grazed Rangelands Using Remote-Sensing-Based Ecological Indicators, Robert A. Washington-Allen, Neil E. West, R. Douglas Ramsey, Carolyn T. Hunsaker

Wildland Resources Faculty Publications

The purpose of this research is to conduct a retrospective assessment of the ecological integrity of a semi-arid landscape subject to commercial livestock grazing. We acquired 27 years of wet and dry season Landsat satellite imagery and combined this with site biological, physical, and administrative characteristics (including historical and current ranch management records) in a Geographic Information System (GIS). A multiple-time by nested multiple-scale experimental design was used to establish causal links between remote-sensing-based ecological indicators, which were diagnostic of rangeland degradation, and management interventions. Rangeland degradation was defined as: (i) a change in plant species composition, contrary to management …

General Catalog 1992 - 1994, Utah State University

University Catalogs

Contains course descriptions, University college calendar, and college administration.

Optimal Linear/Nonlinear, Quantity/Quality Management Of Complex Multilayer Aquifers By Embedding, Ali Gharbi, Richard C. Peralta

Civil and Environmental Engineering Faculty Publications

The embedding optimization modelling approach is adapted to aid long-term groundwater quality and quantity management of complex nonlinear multilayer aquifers. Implicit block-centered finite-difference approximations of the quasi-three-dimensional unsteady flow equation, and Galerkin finite-element approximations of the twodimensional advection-dispersion transport equation are embedded as constraints in the model. Other hydrological processes are also included as constraints. Cyclical linear differencing permits representation of nonlinear transport, even when contaminant is extracted by unsteady pumping (a decision variable). Also used are nonlinear (discontinuous derivative) constraints describing drainage, stream-aquifer interflow and evapotranspiration. The use of both linear and nonlinear formulations of the entire flow and …

Individualistic Perspectives On Plant Competition, Nancy J. Huntly

Biology Faculty Publications

No abstract provided.

Metabolite Production By Entomopathogenic Fungi, Donald W. Roberts, Raymond J. St. Leger, Sandeep Gupta

Biology Faculty Publications

The entomopathogenic fungi produce a wide assortment of metabolites, some of which are important to the host-specialization of this group of fungi. Very few entomopathogenic fungi have been examined in detail for their metabolites in the disease process. Nevertheless, it is clear that certain proteases and lipases are crucial to the invasion of insect cuticle by the fungus. These fungi have the unusual ability to hydrolyze branched alkanes. Also, they normally produce very active, broad-spectrum proteases. These are needed for penetration of the hydrophobic epicuticle and the highly proteinaceous cuticle of insects. Chitinases also are involved, but usually have only …

Fate And Transport Of Tce-Petroleum Hydrocarbon Mixture At Uttr, G. Nash Quashiga, Richard C. Peralta

Civil and Environmental Engineering Faculty Publications

In 1988 a preliminary assessment (PA) study was conducted at Chemical Disposal Pit No. 4 to address several concerns of Hill Air Force Base (HAFB), the facility operators. The objectives of the PA were; i) to assess the potential for organic chemical releases to the subsurface environment, and ii) to determine the need for any immediate control measures, if the subsurface environment were threatened.

Optimizing Perennial Groundwater Yield Planning For Nonlinear Systems: Approach Comparison, Shu Takahashi, Richard C. Peralta

Civil and Environmental Engineering Faculty Publications

Four alternative simulation/optimization models useful for computing optimal sustained-yield (steady-state) groundwater pumping strategies are compared in terms of formulation, solution procedure, accuracy, and computational efficiency. The different models require different computer processing time and memory. For the aquifer tested system, if more than 10% of the cells have pumping as a decision variable, a fully linearized embedding model will require less computer memory than any other model. All the models address linear and nonlinear steady-state flow in multilayer, unconfined/confined aquifers. They also address several types of nonsmooth external flows. Newly presented are a response matrix model solving external flows described …

Optimizing Ground Water Planning And Management, Richard C. Peralta, L. S. Willardson

Civil and Environmental Engineering Faculty Publications

Simulation/optimization (S/0) models can be used to speed the process of computing long term groundwater pumping strategies. These combined models can also greatly ease the planning tasks of water management agencies. They make the process of computing optimal perennial yield groundwater management strategies fairly straightforward. S/0 models dramatically improve conjunctive water management and can help minimize the cost of groundwater contaminant clean-up. The differences between S/0 models and the simulation (S) models currently used by over 98 % of practitioners are illustrated with recent applications of S/0 models in regional planning.