Digital Commons Network^™

Adhesion, Spreading And Fragmentation Of Human Megakaryocytes Exposed To Subendothelial Extracellular Matrix: A Scanning Electron Microscopy Study, Y. G. Caine, I. Vlodavsky, M. Hersh, A. Polliack, D. Gurfel, R. Or, R. F. Levine, A. Eldor

Scanning Electron Microscopy

Platelet agonists and subendothelial extra-cellular matrix (ECM) induce morphological and biochemical changes in animal megakaryocytes, reminiscent of the response of platelets to the same substances. We have examined the behavior of human megakaryocytes exposed for up to 36 hours to the ECM produced by cultured bovine corneal endothelial cells. By phase contrast and scanning electron microscopy these megakaryocytes demonstrated non-reversible adherence and flattening with formation of long filopodia, thus confirming that human megakaryocytes acquire platelet functional capacities. In addition, megakaryocyte fragmentation into prospective platelets was apparently induced by the ECM. Up to 50% of the adherent megakaryocytes underwent spontaneous fragmentation …

Theoretical Considerations On The Effect Of Ion Formation Conditions On The Transmission Through A Laser Microprobe Mass Analyzer, M. De Wolf, T. Mauney, E. Michiels, R. Gijbels

Scanning Electron Microscopy

A theoretical study on the ion transmission through the laser microprobe mass analyzer LAMMA 500 was made using ray-tracing computer programs. The calculations reveal that the ion transmission is strongly affected by the initial conditions of ion formation. Chromatic and spherical aberrations give rise to considerable discrimination in the univoltage lens.

A correlation is attempted between measured and theoretical transmission curves. For the latter a physically plausible plasma model was initially assumed to generate the input parameters, i.e., locus of ion formation and angular and energy distributions of the ions (atomic and cluster ions).

The model needs correction for aberration …

Proton Microprobe Analysis In Biology, Klas G. Malmqvist

Scanning Electron Microscopy

This paper is intended as an introduction to the field of proton microprobe analysis with special emphasis on applications in biological sciences. It is mainly intended for users of electron microscopes equipped with microprobes or other analytical equipment. The basic principles of Particle Induced X-ray Emission analysis are discussed as well as the instrumental requirements for the proton microprobe. The analytical characteristics including quantification procedures are compared with those of the electron microprobe and a review is given of various analytical applications of the proton microprobe within biology and medicine.

Surface-Reconstruction-Induced Changes In Free-Carrier Scattering From The W(100) Surface: Aninfrared Surface-Electromagnetic-Wave Study, D. Mark Riffe, L. M. Hanssen, A. J. Sievers

All Physics Faculty Publications

Using the infrared-absorption technique of surface-electromagnetic-wave spectroscopy (SEWS) we have studied the effects of H₂ and D₂ chemisorption on the SEW attenuation coefficient α in the 10-μm region between 165 and 350 K. The change in α at room temperature has also been measured for N₂, O₂, and CO adsorption. The coverage (CTHETA) dependence of α for N₂, O₂, and CO is fairly simple [either a monotonic increase (N₂) or a peak at some intermediate coverage (O₂, CO)] and points to changes in free-carrier (FC) surface …

Capacitive Coupling Voltage Contrast, S. Görlich, K. D. Herrmann, W. Reiners, E. Kubalek

Scanning Electron Microscopy

Capacitive coupling voltage contrast (CCVC) allows electron-beam testing of passivated integrated circuits (IC) without radiation damage or prior, time-consuming specimen preparation. This effect occurs when low primary electron energies are used and the electron yield of the passivation layer is greater than 1. Signal changes in the relevant interconnections are transferred to the passivation surface via capacitive coupling, but they vanish there within the storage time due to electron irradiation. A physical model explains the dependence of CCVC on three parameters: electron irradiation, the passivation material and the signals within the IC. Computer simulations based on this model describe the …

Using Electron Spectroscopy For Chemical Analysis (Esca) In Failure Analysis: Some Recent Developments, M. A. Kelly, L. H. Scharpen, R. D. Cormia

Scanning Electron Microscopy

Electron spectroscopy for chemical analysis (ESCA) is receiving increased attention as a surface-sensitive failure analysis technique because of several recent developments. These are: first - substantially improved spatial resolution, allowing a much broader range of problems to be studied; second - new ways to apply analytical results to practical problems, resulting from better technical understanding of ESCA and better data reduction techniques; and third - the development of sample handling systems which can handle large, outgassing samples, making ESCA a relatively non-destructive technique.

In this paper we consider ESCA's niche as a surface analysis tool, and discuss the impact of …

The Morphologic Categorization Of Cell Death Induced By Mild Hyperthermia And Comparison With Death Induced By Ionizing Radiation And Cytotoxic Drugs, David J. Allan, Brian V. Harmon

Scanning Electron Microscopy

This paper presents 1. a summary of the morphological categorization of cell death, 2. results of two in vivo studies on the cell death induced by mild hyperthermia in rat small intestine and mouse mastocytoma, and 3. a comparison of the cell death induced by hyperthermia, radiation and cytotoxic drugs.

Two distinct forms of cell death, apoptosis and necrosis, can be recognized on morphologic grounds. Apoptosis appears to be a process of active cellular self-destruction to which a biologically meaningful role can usually be attributed, whereas necrosis is a passive degenerative phenomenon that results from irreversible cellular injury.

Light and …

Electron Detectors For Electron-Beam Testing Of Ultra Large Scale Integrated Circuits, S. C. J. Garth, D. F. Spicer

Scanning Electron Microscopy

The increasing density of components in integrated circuits imposes severe constraints on conventional electron beam testers. The use of electron detectors consisting of combined electrostatic and magnetic fields has demonstrated improved performance over conventional electrostatic detectors. Such detectors also ease many of the practical difficulties associated with electron beam testing of Ultra Large Scale Integration (ULSI) circuits. A detector using a single pole magnetic lens will be described and compared to detectors which use only electrostatic fields. The single pole lens detector has demonstrated superior performance to the electrostatic detector in terms of local field error and imaging resolution, allowing …

Charging Effects In Low-Voltage Scanning Electron Microscope Metrology, M. Brunner, R. Schmid

Scanning Electron Microscopy

Low voltage operation of the scanning electron microscope is being increasingly used to avoid negative charging in e-beam inspection and metrology. Positive charging effects, however, may still disturb the measurement accuracy even with low primary beam energies. Current investigations have revealed that no errors due to positive charging occur on resist structures on semiconductor substrates. But samples with metal structures on insulating substrates do involve disturbing effects due to positive charging. The difference in behavior between these groups of samples is attributed to the fundamental difference between insulator and conductor charging. This difference is due to different field geometries on …

Osmium Vapor Pretreatment Of Gnomonia Infected Leaves, C. R. Krause, J. M. Ichida, L. S. Dochinger

Scanning Electron Microscopy

A study was conducted to determine if pretreatment with osmium tetroxide (OsO₄) vapor prior to the conventional preparation procedure would increase the retention of fungal structures on leaf surfaces as observed with scanning electron microscopy (SEM). Leaves of black walnut (Juglans nigra L.) were inoculated with conidia of Gnomoma lepfostyla (Fr.) Ces. and de Not., the etiological agent that causes anthracnose of walnut. Following lesion development, leaves were either conventionally prepared with immersion in fixative, ethanol and critical-point dried or vapor-fixed with OsO₄ before conventional specimen preparation. Data indicate that significantly more fungal structures were …

Applications Of X-Ray Microanalysis To The Study And Conservation Of Ancient Glasses, S. Hreglich, M. Verita

Scanning Electron Microscopy

X-ray microanalysis can be successfully applied to the study of the chemical composition and weathering processes of ancient glass. The problems concerned with this kind of application are considered. A verification is conducted of the accuracy and sensitivity of this technique, which are sufficient to obtain useful information on technologies and raw materials used and consequently on the origin and dating of ancient glasses.

This analytical method also discloses meaningful data for the optimization of restoration and the choice of appropriate conservation operations.

High Resolution Scanning Auger Microscopy Of Mineral Surfaces, M. F. Hochella Jr., A. M. Turner, D. W. Harris

Scanning Electron Microscopy

There are a number of cases where scanning Auger microscopy can be used to determine the near-surface composition of minerals with extremely high lateral resolution. This involves collecting Auger spectra with reasonable signal to noise ratios without encountering significant beam induced charging or surface degradation, even if the beam is impinging on a grain less than 1μm in diameter. We typically use a 3 keV beam with less than 10 nA beam current on a sample surface that is tilted (to increase backscattered and secondary electron emission efficiency) and relatively flat. To further minimize surface degradation, vacuum levels are kept …

Quantitative Ion Microprobe Analysis Of The Rare Earth Elements In Minerals, Ghislaine Crozaz, Ernst Zinner

Scanning Electron Microscopy

The lanthanides or rare earth elements (REE) which are present in trace concentrations in rocks are most useful for investigating the origin of these objects. Of the microbeam techniques presently used to measure the REE concentrations of individual crystals, secondary ion mass spectrometry (SIMS) is the only method sensitive enough to allow the determination of REE abundances in most natural minerals. Usually, energy filtering is applied to remove all complex molecular interferences. All the REE, down to a level of ≤ 100 ppb, can be measured in spots 5 to 20 𝜇m in diameter. A growing number of studies involving …

Scanning And Transmission Electron Microscopic Study Of Recovered Porcine Aortic Valved Conduits, D. J. Allen, I. H. Fentie, J. T. Davis, Angela Lineen

Scanning Electron Microscopy

Four explanted porcine aortic valved conduits were examined using scanning and transmission electron microscopy. Sources of obstruction such as neointima or "peel" and calcification were observed. In one sample the neointima was found to possess an unusually large expanse of squamous cells partially lining the luminal surface. This lining much resembled a normal endothelium, which is not an expected feature of neointima. Cells, presumably of host origin, were noted upon the leaflet surfaces. They did not seem as well organized as those found on the neointima. Calcification did not seem greatly advanced but was clearly apparent. Certain treatments proposed by …

Statoconia Formation In Molluscan Statocysts, Michael L. Wiederhold, Christine E. Sheridan, Nancy K. R. Smith

Scanning Electron Microscopy

The gravity sensors of all molluscs phylogenetically below the cephalopods are spherical organs called statocysts. The wall of the sphere contains mechanosensory cells whose sensory cilia project into the lumen of the cyst. The lumen is filled with fluid and dense "stones", the statoconia or statoliths, which sink under the influence of gravity to load, and stimulate, those receptor cells which are at the bottom. The statuconia of Aplysia californica are shown to be calcified about a lamellar arrangement of membranes. Similar lamellar membrane arrangements are seen within the receptor cells, and their possible role in the formation of the …

Cells From Xenopus Laevis Gastrulae Adhere To Fibronectin-Sepharose Beads And Other Lectin Coated Beads, Kurt E. Johnson, Michael H. Silver

Scanning Electron Microscopy

Cells from Xenopus laevis blastulae have a poorly developed ability to adhere to Sepharose beads covalently coupled to bovine plasma fibronectin (FN-beads). They do, however, have the ability to adhere to con A-beads and cytodex-1 and cytodex-3 beads. Beginning at the early gastrula stage, there is a progressively increasing ability of cells to adhere to FN-beads. Gastrula cells adhere to FN-beads by the formation of large ruffling lamellipodia. These cells can translocate on the surface of FN-beads; and when attached to both beads and the surrounding glass substratum of culture vessels, have the ability to move the beads extensively. Gastrula …

Data Collection And Presentation Procedures For The U.S. Army's Chemical Hazard Assessment Warning System (Chaws), Michael H. Merry

All U.S. Government Documents (Utah Regional Depository)

This report describes the basic procedures and references more detailed documents for the operation of meteorological and air quality sensing instrumentation, data depiction and analysis systems for an incinerator pilot plant located at the Tooele Army Depot site near Salt Lake City, Utah. It discusses the site topography and climatology, sensor configuration, calibration, quality assurance and data reporting procedures for the monitoring system and demonstrates their compliance with applicable environmental regulations of United States Federal Government and the State of Utah.

Weighted Silks Observed Using Energy Dispersive X-Ray Spectrometry, M. Ballard, R. J. Koestler, N. Indictor

Scanning Electron Microscopy

A group of modern silk fabrics was treated with various weighting agents as standards. These standards were observed using energy dispersive x-ray spectrometry. The standards were compared to untreated samples and to museum specimens of weighted silks. In all prepared specimens the elements expected from treatment were observed; weighting treatments could be distinguished. The analytical procedures are discussed and compared to recent work on mordant analysis using the same techniques.

Simulating A Large Wasatch Front, Utah, Earthquake Using Small Earthquake Recordings As Green's Functions, United States Department Of Defense, Air Force Geophysics Laboratory

Geology

Several earthquake recordings at Golden, Colorado in the magnitude range 4-6, were digitized and were used to investigate the feasibility of adding them together to simulate a larger earthquake (magnitude 7.0-7.5). The test path of the Wasatch Front, Utah to the WWSSN station at Golden was selected (distance=400-500 km). The hypothetical causative fault was given dimensions on the order of 45 km long by 20 km wide and was divided into "cells," representative in size to the "seed" events rupture dimensions and with other variations. The results were evaluated by total energy and amplitude criteria. The final waveforms were judged …

Quantitative Vascular Casting Of The Post-Ischemic Hydronephrotic Kidney, Vincent H. Gattone Ii, Robert D. Sale

Scanning Electron Microscopy

The renal microvasculature (afferent arteriole) and glomeruli were examined and quantitated by two methods in the post-ischemic hydronephrotic (PIH) kidney. The methods used were: 1) an in vivo examination and 2) controlled perfusion-fixation, quantitative vascular casting examined by scanning electron microscopy. The second method was also applied to the vasculature of the contralateral, functional kidney. The goals of the study were to: 1) validate the quantitative vascular casting method by comparing PIH renal microvascular data from the casting method with in vivo values and 2) determine the extent of microvascular dimensional difference of the PIH kidney from its contralateral functional …

Immunoarchitecture Of The Regenerating Rat Spleen: Effects Of Partial Splenectomy And Heterotopic Autotransplantation, Michael C. Dugan, Thomas M. Grogan, Marlys H. Witte, Catherine Rangel, Lynne Richter, Charles L. Witte, David B. Van Wyck

Scanning Electron Microscopy

To investigate the microstructure of in situ (eutopic) and autotransplanted (ectopic) splenic remnants, adult Sprague-Dawley rats were studied 60 days after 1) subtotal (~80%) splenectomy, 2) total splenectomy followed by single or multiple remnant intraperitoneal autotransplantation, or 3) sham operation. Total nucleated cell counts were determined in excised splenic remnants, and immunohistochemical staining using monoclonal antibodies to rat B-and T-cell antigens was performed in serial tissue sections.

Immunoarchitecture of eutopic remnants was indistinguishable from that of intact spleens and total nucleated cell counts remained proportional to weight. In contrast, ectopic remnants showed sparsity and abnormal mixing of B and T …

Use Of Electron Back Scatter Diffraction Patterns For Determination Of Crystal Symmetry Elements, D. J. Dingley, Karim Baba-Kishi

Scanning Electron Microscopy

The application of electron back scatter diffraction in the scanning electron microscope has been extended to the determination of crystal symmetry elements, point group and space group. The wide angular range of the patterns makes this a relatively simple task compared with equivalent analysis using electron channelling patterns, convergent beam patterns or standard x-ray methods, though the complexity of the analysis does not permit an unthinking approach. To establish the best procedure specimens from the seven crystal systems were investigated and results from the examination of the metal tin (tetragonal), and minerals zircon (ZrSiO₄, tetragonal) and calcite (CaCO …

Solar- Terrestrial Physics: A Space Age Birth, R. W. Schunk

Faculty Honor Lectures

Solar- Terrestrial Physics, in its broadest sense, is concerned with the transport of energy, particles, and fields from the sun to the earth and their consequent effect on the terrestrial environment. Most of the solar energy eventually deposited in our atmosphere, at a rate of approximately a trillion megawatts, arrives in the form of visible light. The study of how this energy affects our environment falls within the purview of meteorology, a discipline that has experienced an independent development and that has sufficiently different problems from solar-terrestrial physics that it can be regarded as a separate but neighboring discipline. In …

Plant-Herbivore Interactions: Grasses And Grasshoppers In Grand Canyon National Park, Arizona, Tim B. Graham

Biology

In the Grand Canyon, grasses with 3 carbon photosynthetic pathway and C₄ grasses have different distribution patterns: C₄ grasses are more abundant at lower elevations, C₃ species are more common at higher elevations. Differences in C₃ and C₄ grass abundances at different sites were used to test the following hypotheses using three species of grasshopper: 1. C₄ plants are avoided by herbivores; and 2. sparse grasses are preferred as food over abundant grasses by herbivores. Food habits of the grasshoppers were analyzed using carbon isotope ratios and feeding trials. Carbon isotope analysis gave estimates …

Intercept Feeding As A Means Of Reducing Deer-Vehicle Collisions, Peggy Wood

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Intercept feeding was tested for its efficacy in reducing deer-vehicle collision frequency by diverting deer movement patterns away from highways using alfalfa hay as an attractant. Ratios of roadkills in control vs. treatment zones of three highway segments indicated that feeding reduced collision frequency. Spotlighting counts of live deer were significantly higher (P < 0.001) in the control than in the treatment zone, supporting the effectiveness of feeding in keeping deer distant from the highway. In a benefit:cost analysis, the benefits accrued by reducing collision frequency exceeded the costs of feeding on each highway both years, with one exception: in 1986 one of the highways demonstrated that particular features of an area may not be conducive to effectively attracting deer away from a highway. I recommend intercept feeding be used with alternate techniques, so that the most appropriate technique is applied in each situation to reduce collision risk.

Belowground Resource Exploitation In Semiarid Plants: A Comparative Study Using Two Tussock Grasses That Differ In Competitive Ability, David M. Eissenstat

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

The relative competitive abilities of Agropyron desertorum and Agropyron spicatum were compared using Artemisia tridentata transplants as indicator plants. Although these two tussock grasses have similar shoot growth forms and shoot physiological characteristics, they have substantial differences in their competitive abilities. Artemisia had lower survival, growth, reproduction, and water potential when transplanted into neighborhoods of A. desertorum than in neighborhoods of A. spicatum.

Plant attributes associated with the differences in competitive ability were explored. Agropyron desertorum and A. spicatum have remarkably similar potential growth rates at warm soil temperatures. In a prolonged cold soil temperature treatment in the greenhouse, …

Parental Attitudes Toward Mainstreaming Of Handicapped Children, Afsaneh Ahooriyan

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

The primary purpose of this study was to investigate attitudes of parents of school-aged and preschool aged children with and without handicaps, toward the issue of mainstreaming. A sample of 212 individuals responded to a parent questionnaire which was mailed to each household to be completed by both parents. This questionnaire examined attitudes and concerns of parents across 14 mainstreaming issues (Appendix 2) . Of the total sample, 123 responses were used for the final analysis. Cases were discarded because some were missing the age of their child, some were not qualified because of their child's age, and others had …

Marriage Enrichment: Does It Make A Difference, Eric R. Olsen

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

The purpose of this study was to determine if participation in a marriage enrichment program had an impact on couple's relationships in the areas of regard, empathy, congruence, dyadic adjustment and self-esteem. Seven independent variables were also analyzed to see if they had any impact on the five outcome measures. A survey was given at four different time periods (pretest, immediately before the enrichment experience; posttest, immediately after the enrichment experience; three months following; and six months following the enrichment experience) to determine if change did occur and if it was maintained over time. There was no control group and …

Manganese Toxicity In Mice: Evaluation Of Neruochemical And Immunologial Alteration, Benjamart Srisuchart

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Neurochemical and immunological effects of manganese chloride (MnCl₂) were evaluated using young male CD-1 mice as the animal model. Intraperitoneal (i.p.) injection of MnCl₂ (1, 3 and 10 mg/kg) daily for 4 weeks did not produce mortality or overt signs of toxicity in any of the treated animals. Manganese treatment increased the thymidine uptake of cultured splenic cells and the selective lymphoproliferative response to T-cell mitogens, phytohaemagglutinin and concanavalin A, in all the treated groups. No difference in the response to B-cell mitogen, lipopolysaccharide was observed. A dose of 10 mg/kg MnCl₂ increased the mixed lymphocyte …

The Role Of Vicarious Reinforcement For Modeled Or Alternate Behavior, Brian C. Lech

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Research on vicarious reinforcement has answered many questions but whether vicarious reinforcement increases the likelihood that an observer will imitate a model, a social learning theory would predict, or sets the occasion for the observer to perform an alternate response, as a discriminative stimulus interpretation of vicarious reinforcement suggests, seems to depend on (1) the setting, (2) procedure, and (3) reinforcers used. In an effort to better understand the function of vicarious reinforcement, while controlling for subjects; histories and using tangible reinforcers, 47 preschool children participated in two experiments that (1) provided an experimental history of responding on several levers, …