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Scanning Transmission Ion Microscopy As It Complements Particle Induced X-Ray Emission Microanalysis, H. W. Lefevre, R. M. S. Schofield, J. C. Overley, J. D. Macdonald

Scanning Microscopy

The early uses of Scanning Transmission Ion Microscopy (STIM) with MeV ions are reviewed. The transformation of STIM energy-loss images into maps of areal density is discussed, and is illustrated with images of a fruit fly head (Drosophila melanogaster). Freeze-dried male heads are transparent to 4-MeV protons in the dorsal and frontal directions, but in the sagittal direction the brain is opaque. STIM with molecular ions is shown to be useful for increasing contrast in low density areas. For recording registered STIM and PIXE images without changing accelerator parameters, apertures are used to accomplish the required change in beam intensity …

Ultrastructural Analysis Of Dynamic Cellular Processes: A Survey Of Current Problems, Pitfalls And Perspectives, Helmut Plattner, Gerd Knoll

Scanning Microscopy

Dynamic phenomena in cells that can be analyzed on the ultrastructural level comprise so different aspects as ion shifts, conformational changes of macromolecules, membrane particle rearrangements, lipid phase transitions, protein--protein interactions (notably ligand-receptor interactions, including their sorting and sequestration), reversible membrane-to-membrane contacts, membrane fusions, transcellular transport phenomena, restructuring of cytoskeletal elements, ciliary and flagellar beat, cell shape changes, etc. Only some of these phenomena can be analyzed under stationary conditions, while others are unidirectional and sometimes very rapid. Therefore, the methodical approaches to be used (primary methods and follow-up procedures) might be widely different. Quite different methods are available, such …

Immunogold Localization Of Intra- And Extra-Cellular Proteins And Polysaccharides Of Plant Cells, Stuart Craig, Patricia J. Moore, Terri G. Dunahay

Scanning Microscopy

This paper illustrates post-embedding immunogold labelling of protein and polysaccharide molecules of plant cells. For EM studies, one is restricted (for most plant cells) to the post-embedding approach because the surrounding cell wall prevents access of antibodies (and secondary gold-tagged markers) to internal sites. The large size of many plant cells also does not lend itself to diffusional entry of antibodies. The molecules localized include seed storage proteins that are large and present in major quantities, a smaller less abundant, water soluble albumin, an oxygen-binding protein, components of the photosynthetic electron transport chain, and complex sugars from the cell wall. …

Low Temperature Techniques In Biomedical Microanalysis, Romuald Wróblewski, Joanna Wroblewski, Godfried M. Roomans

Scanning Microscopy

Many diseases are associated with a change in the distribution of diffusible ions at the cell or tissue level. These diseases can profitably be studied by X-ray microanalysis. This technique for the study of ion distribution requires the use of cryoprepared specimens. Analysis at low or medium resolution can be carried out on thick or semi-thick cryosections, or on frozen-hydrated or freeze-dried embedded bulk samples. Such analyses are particularly useful in the initial stages of an investigation, or when many data from a large number of samples have to be acquired. Quantitative analysis is then usually carried out with the …

Shape From Shading Using Multiple Detector Signals In Scanning Electron Microscopy, L. Reimer, R. Böngeler, V. Desai

Scanning Microscopy

Using a two-or multiple detector system for secondary electrons (SE) or backscattered electrons (BSE) the difference signal can be used to reconstruct the surface profile. Micrographs re-corded by these difference signals are more like shaded images obtained by illumination from one side than conventional micrographs using only one detector. Therefore, the concept of the shape-from-shading method developed for light illumination can be transferred to scanning electron microscopy as long as one considers the characteristic differences in signal detection and image formation. The surface tilt contrast causes signal differences A-B when using a two-detector system of opposite Everhart-Thornley or semi conductor …

Monte Carlo Calculations Of Electron Emission At Surface Edges, L. Reimer, D. Stelter

Scanning Microscopy

A Monte Carlo method using Mott cross-sections for elastic scattering and a modification of the Bethe continuous-slowing-down by inelastic scattering at inner- shell electrons has been used to calculate linescans across surface edges and steps using a two-detector system for SE and BSE with exit momenta to the right (detector A) and to the left (detector B). The step height h=10R, R, R/2, R/5 and R/10 (R = electron range) and the inclination angles 𝛂=30°- 80° of edges and steps have been varied to get information about the influence of these quantities on the linescans. The signals contain contributions by …

The Use Of Scanning Electron Microscopy In Studying Enamel Caries, R. P. Shellis, A. S. Hallsworth

Scanning Microscopy

SEM studies related to carious change in dental enamel are reviewed, and their contribution to understanding the mechanism of formation of the early enamel lesion and of its repair evaluated. SEM has contributed significantly to understanding the mechanism of enamel dissolution at the level of the single crystal. Etching studies have yielded useful information on the effect of enamel structure on the pattern of acid dissolution at the microscopic level and have highlighted the importance of re- precipitation phenomena in modifying the pattern of mineral loss. High-resolution studies have provided interesting quantitative data on changes in crystal size, and also …

A Revision Of The Genus Nomada Subgenus Nomadita Of North America (Hymenoptera: Anthophoridae), Derek Kyle Broemeling

Biology

The North American portion of the subgenus Nomadita of the genus Nomada is revised. Six species and 2 subspecies are recognized, including the new species timberlakei and the new subspecies snowii arriolaense. Nomada antonita Cockerell is placed in synonymy with snowii snowii. Nomada rodecki Mitchell is removed from the subgenus Nomadita and placed in the subgenus Nomada. The Eurasian species of Nomadita are discussed. A new subgenus, Asteronomada is described based upon Nomada adducta Cresson with new species brewsterae, durangoae, and portalensis described.

Resistance Frequency To Trichlorfon And Permethrin In Lygus Hesperus Knight (Hemiptera: Miridae) Populations Estimated By Bioassays, Electrophoresis, And Colorimetric Tests, Boubacar Hassane

Biology

Monitoring and early detection is critical to resistance management. Diagnostic tests such as colorimetric spot tests and electrophoresis are very efficient techniques in accurately distinguishing between resistant and susceptible individuals. All biological assays were conducted by simple contact exposure in vials containing different concentrations of trichlorfon and/or permethrin (0.01 to 30ug/vial). Vials used for control in bioassays were treated with acetone only. Synergist ratio (SR), percent synergism (%S) and log percent synergism (L%S) were calculated to determine synergism effects by four synergists: S, S, S-tributyl phosphorotrithioate (DEF), diethyl maleate (DEM), piperonyl butoxide (PB) and triphenyl phosphate (TP). DEF proved to …

Studies Of The Proteinase Activities Of Streptococcus Lactis And S. Cremoris, Fahad Ali Khayat

Biology

Twelve proteinase positive (Prt+) and twelve proteinase negative (Prt-) variant pairs of Streptococcus cremoris and three Prt+ and three Prt- variant pairs of S. lactis were assayed for proteolysis in milk using spectrophotometric o-phthaldialdehyde (OPA) and trinitrobenzene sulfonic acid (TNBS). Both methods separated Prt+ and Prt- cultures. The TNBS data range was wider between Prt+ and Prt- strains and thus greater differentiation was possible. The correlation of determination (R2) between OPA and TNBS methods was 0.88 with a coefficient of variation (CV) within of 2.2 and 3.0%. The data were compared to a cell mass increase method. The R2 between …

An Analysis Of Dna Polymorphisms In Hereditary Multiple Exostoses, Brian K. Moloney

Biology

Hereditary multiple exostoses (HME) is a bone disease characterized by multiple benign bone tumors which develop only during active skeletal growth. Previous studies have demonstrated an autosornal dominant pattern of inheritance. In this study, several cellular oncogenes and human chromosomal fragments were used as probes to identify any restriction fragment length polymorphisms in the genomes of HME affected horses and humans. Sequences homologous to the human oncogenes c-fes, c-myb, c-myc, c-raf-1 and c-sis were identified in the horse genome. Polymorphisms were identified using c-raf-1 hybridized to Bam HI and Pst I digested horse DNA, but neither appeared linked to HME. …

The Feasibility Of Artificial Insemination Of Dairy Cattle Managed By Fulani Tribesmen In Kaduna State, Nigeria, Christian Olafimihan Ojomo

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

This study sought to determine: (a) the essential elements of a viable artificial insemination program for the Fulani Tribesmen in Kaduna State, Nigeria, and (b) to determine if the essential elements of a viable artificial insemination program exist in Kaduna State, Nigeria.

This study was conducted from April 15 to May 15, 1985, in the Artificial Insemination (AI) Subcenters of Zaria, Katsina, Kabomo, Kaduna, Ikara, Zonkwa, Kurmin-Biri, and Kauranjuli.

The data presented in this study were collected by personal interviews with Fulani Tribesmen. The population of this study was from a partial list of Fulani Tribesmen who have had AI …

A Study Of The Relationships Between Hemispheric Asymmetries And Intellectual Abilities, Anne Mcshane

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

This study investigated the functional significance of cerebral asymmetries. Width measurements of the human brain were derived from computerized tomographic (CT) films and related to intellectual variables as determined by the Wechsler Adult Intelligence Scale Revised (WAIS-R). Subjects were adults of both sexes who had been referred for neurologic examination and were diagnosed as having no abnormalities (N=28). Reasons for referral included headache, dizziness, or to rule out central nervous system damage following various types of trauma. The asymmetry of hemispheric widths (left minus right) in the frontal, temporoparietal, and occipital areas was correlated with Verbal IQ minus Performance IQ …

Disturbed Alpine Ecosystems: Seedling Establishment Of Early And Late Seral Dominant Species, Jeanne C. Chambers

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

This study examined the effects of seedbed and seedling environment on establishment of early and late seral dominant alpine species. Species studied included late seral dominant forbs (Geum rossii, Artemisia scopulorum, and Polemonium viscosum), early seral dominant forbs (Potentilla diversifolia and Sibbaldia procumbens), a late seral dominant grass (Festuca idahoensis), and early seral dominant grasses (Calamagrostis purpurascens and Deschampsia cespitosa). Germination responses of each species to wet vs. dry cold stratification and light vs. dark conditions were investigated. No statistical differences were observed in the seed germination of early and late …

Cottage Cheese From Ultrafiltered Skimmilk By Direct Acidification, Jorge Ricardo Ocampo-Garcia

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Pasteurized skimmilk at 4°C was acidified to pH 5.8 with 85.5% phosphoric acid (136g H₃PO₄/100 kg skimmilk), then warmed to 54°C and ultrafiltered to a protein concentration 9.1 ± 0.2%. The retentate was heated to 76.5°C for 16 s then cooled to 2°C. Phosphoric acid (85.5%) was added at a rate of 3.41g per kg retentate. The acidified retentate was slowly warmed to 29.5°C (3°C/5 min) when the pH was checked. The pH at this point was no lower than 5.4. Heating was continued until a temperature of 32.2°C was reached. Glucono delta lactone was added …

Expert System Technology And Concept Instruction: Training Educators To Accurately Classify Learning Disabled Students, Mary Anne Prater

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Many learning disabled student being served by the public school systems have been inaccurately classified. Training and research efforts are needed to assist members of the multidisciplinary team in making more accurate learning disabilities classification decisions.

CLASS.LD2, a computer-based expert system, was designed to assist multidisciplinary teams by providing second-opinion advice regarding the appropriateness of a learning disabilities classification for individual student cases. The existing expert system, CLASS.LD2, was combined with strategies for effective concept instruction to create an instructional package entitled LO.Trainer.

The purpose of this study was (a) to develop a computer-based instructional package combining expert system technology …

The Effect Of An Early Sensorimotor Intervention Program On The Development Of Infants With Perinatal Intraventricular Hemorrhage, Janet Millard

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Our current ability to identify and appropriately treat infants who are at risk for developing various handicapping conditions is limited. Thus, research aimed at developing early diagnostic techniques and differential intervention programs for infants at risk for handicaps needs further attention. The purpose of this study was to determine if infants who suffered perinatal intraventricular hemorrhage and who received routine medical care plus sensorimotor intervention between 3 and 12 months of age, differed from similar infants who received only routine medical care.

Twenty-four subjects (10 experimental and 14 control) who were patients in neonatal intensive care at University of Utah …

The Effects Of Reprimands And Work Observation With Pay Loss On The Maintenance And Generalization Of High-Quality Work By Developmentally Disabled Adults In Community Work Settings, Benjamin Lignugaris/Kraft

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

The purpose of these studies was to examine the effects of reprimands, a mild disciplinary procedure, and work observation and pay loss, a severe disciplinary procedure on the maintenance of high-quality work among mild developmentally disabled individuals employed as housekeepers. In general, participants were more responsive to reprimands after work observation and pay loss was applied. In addition, the increased sensitivity to reprimands appeared to generalize to other work settings for one participant. While participants' work quality varied across conditions, their work rate was relatively stable. These results are discussed in terms of other research that examined the use of …

Pms: Bi-Phasic Differences In Personality And Marital Relations Among A Clinical Sample, Donna R. Rogers

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Premenstrual Syndrome (PMS) is a controversial and widely misunderstood syndrome which encompasses mood, behavior, and physical symptoms that occur cyclically and are associated with the menstrual cycle. Many women report suffering from recurring PMS symptoms severe enough to create a temporary physical or mental incapacitation which may affect the marital relationship. A study was initiated to document bi-phasic personality and marital changes related to PMS.

This sample consisted of 119 adult females and their husbands, ranging in age between 18 and 60, who sought diagnosis and treatment at the Utah PMS Center in Salt Lake City, Utah. Within this clinical …

Parent-Adolescent Sexual Attitude Congruence In Relation To Adolescent Sexual Intercourse Experience, John Kelly Mccoy

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Survey data from 472 adolescents (ages 14-19) and their parents were analyzed to examine the relationship that existed between the premarital sexual attitudes of parents and the sexual behavior of their adolescents. The attitudes of the adolescents and their parents were measured using Likert type questions about sexual Intercourse prior to marriage. The sexual behavior of adolescents was based on the responses of the adolescents regarding their frequency of necking, petting, and sexual Intercourse. The relationship between adolescents' sexual behavior and parents' attitudes was examined using several different conceptual models. The amount of attitude agreement that mothers and fathers had …

Comparing Different Methods For Determining Moisture In Wheat Using Nesr Infrared Reflectance Spectroscopy, Weider Chang

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

One hundred wheat samples, with moisture ranging from 14.42 %to 7.66 %, were used to test the moisture loss from grinding, the accuracy of moisture content from two different electric moisture meters, and the accuracy and precision of Near Infrared Reflectance Spectroscopy whole wheat moisture determination.

Fifty samples were ground through three grinders, Wiley, Cemotec, and UDY Cyclone grinder. Moisture content was determined from these ground samples, and compared with whole wheat moisture. The Cemotec grinder caused the least amount of moisture loss, followed by Wiley and then UDY Cyclone.

Fifty whole wheat samples were used to calibrate the Tecator …

Effects Of Sire, Ration, And Interaction Of Sire With Ration On Reproductive Performance Of Holstein Dairy Cows, Jen-Hon Justin Chen

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

A study was conducted to analyze reproductive data gathered over a ten-year period at the Utah State University Dairy Farm. The study utilized 289 complete first lactations of Holstein cows, including 150 daughters of 10 sires in Trial I and 139 daughters of 8 sires in Trial II. One sire was used in both trials; this was sire 4 in Trial I and sire 18 in Trial II. The study measured ration, season, and sire effects and their interactions on the reproductive performances of dairy cows.

Reproductive traits analyzed were: days from calving to first estrus, days from calving to …

Economic Aspects Of Reproductive Problems In Utah And Southeastern Idaho Dairy Herds, Steve Lemrick

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

One hundred eighty-seven survey questionnaires asking for reproductive information for 1985 were sent to dairymen in Utah and Southeastern Idaho. Forty- two herds with current DHI information and whose owners or managers completed and returned the questionnaire were used to estimate economic losses due to reproductive problems, especially excessive days open, in Utah and Southeastern Idaho.

Average days open for herds surveyed were 122.4 days with a standard deviation of 22.6 days, and a range of 89 to 177 days. Several factors were significantly correlated with days open including services per conception, previous calving interval, and missed heats. Missed heats …

An Application Of The Finite Element Method And Two Equation (K And E) Turbulence Model To Two And Three Dimensional Fluid Flow Problems Governed By The Navier-Stokes Equations, John I. Finnie

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Finite Element computer codes in two and three dimensions were written that solve both laminar and turbulent flow. These codes use the two equation (k and E) turbulence model to evaluate turbulent viscosity. They were tested with 29 different flow problems. The largest two dimensional turbulent problem solved is flow under a sluice gate. A three dimensional vortex flow problem was attempted but was not feasible due to the size of the available computer. The Harwell sparse matrix subroutines of the United Kingdom Atomic Energy Authority were used to solve the set of simultaneous equations. The performance of these subroutines …

Least-Change Secant Updates Of Non-Square Matrices, Samih Kassem Bourji

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

In many problems involving the solution of a system of nonlinear equations, it is necessary to keep an approximation to the Jacobian matrix which is updated at each iteration. Computational experience indicates that the best updates are those that minimize some reasonable measure of the change to the current Jacobian approximation subject to the new approximation obeying a secant condition and perhaps some other approximation properties such as symmetry.

All of the updates obtained thus far deal with updating an approximation to an nxn Jacobian matrix. In this thesis we consider extending most of the popular updates to the non-square …

The Relative Susceptibilities Of Interresponse Times And Post-Reinforcement Pauses To Differential Reinforcement, Nancy L. Trapp

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Post-reinforcement pauses (PRP) and interresponse times (IRTs) were examined to determine if these two temporal units changed in a similar fashion as a function of the delivery of differential reinforcement. Two experiments were conducted. In Experiment 1, four pigeons were exposed to a series of procedures in which PRP and IRT durations were gradually increased and then decreased. A fixed-ratio two (FR 2) differentiation schedule was used. Reinforcement was delivered if the PRP or IRT durations were greater than (PRP > and IRT > procedures) or less than (PRP < and IRT < procedures) specified temporal criteria. Criteria were gradually changed across procedures. Results showed that PRPs and IRTs changed in accordance with the differential reinforcement as specified by the various contingencies. When PRPs and IRTs were free to vary, the PRPs tended to change in a direction consistent with the IRT shaping contingencys whereas, the IRTs tended to shorten regardless of the PRP shaping contingency. In Experiment 2, two subjects were exposed to both an FR 2 and FR 1 schedule to determine if schedule size influenced the effects obtained on the differentiation procedures. PRPs were systematically changed using a differentiation procedure with a response requirement of either FR 1 or FR 2. Results showed similar changes in PRP durations between FR 1 and FR 2 differentiation procedures. An analysis of errors made on each shaping condition in both experiments was conducted to determine whether PRPs or IRTs were more susceptible to the differential reinforcement contingencies. Fewer errors were made on the PRP shaping conditions, indicating that PRPs were more easily changed. Implications for a comprehensive theory of reinforcement were discussed.

Multiple-Stimulus Discrimination And Stimulus Overselectivity With Preschool Children, Maria C. Quintero

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Stimulus overselectivity is said to occur when behavior is under the control of a restricted set of stimuli from a stimulus complex. Three studies investigated the effects of specific multiple-stimulus training histories upon the overselective responding of normal preschool children.

In Experiment 1, eight children 3 to 5 years of age, were trained to discriminate forms presented on cards. Each form was labelled with a nonsense syllable, and each card (multiple stimulus) consisted of two forms. A time-delay training procedure was used. Four subjects were trained using Concurrent training in which two of the three S- response choices contained components …

Attempts At In Vitro Synthesis Of The B875 Light Harvesting Polypeptides Of Rhodobacter Sphaeroides Using Recombinant Escherichia Coli Plasmid Pss2, Kevin Seeley

Biology

The purpose of this work was to attempt to synthesize, in vitro, the B875 light harvesting proteins of Rhodobacter sphaeroides from a PBR322 derived recombinant plasmid named pSS2. Large scale plasmid purification of both the recombinant plasmid and pBR322 was performed. Restriction digest patterns indicated that the extracted plasmids were the correct size and construction. An in vitro DNA directed-coupled transcription-translation protein synthesis was developed from extracts of either R. sphaeroides or Escherichia coli. Trichloroacetic acid (TCA) protein precipitation techniques were used to determine the protein synthesis activities of the extracts and plasmids. The concentration optimum for magnesium …

Petrology Of The Middle Cambrian Langston And Ute Formations In Southeastern Idaho, Daniel T. Rogers

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

The Middle Cambrian Langston and Ute formations were studied in the northern portion of the Bear River Range and the south-central part of the Portneuf Range in southeastern Idaho.

The rocks of the Langston and Ute formations were divided into 17 different rock types. The 17 rock types were formed within four recognizable lithofacies: I) Shoal - or Coastal-Peritidal Carbonate Complex; 2) Agitated Shoal; 3) Inner Marine Shelf; and 4) Outer Marine Shelf. Clastic sediments belonging to the Spence Shale Member of the Langston Formation (outer marine shelf) were deposited over the carbonate complex. A transgressive sequence marks the base …

Mechanism Of Iron Transport In Mycelia Sterilia Ep-76, Jonathan P. Adjimani

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

The cyclic trihydroxamic acid, N, N', N' '-triacetylfusarinine C, produced by Mycelia sterilia EP-76, is shown to be a ferric ionophore for this organism. The association constant for ferric-N, N', N' '-triacetylfusarinine C complex was determined to be log K=32.5. Other iron chelating agents, such as rhodotorulic acid, citric acid, or the monomeric subunit of triacetylfusarinine C, N-acetyl- fusarinine, delivered iron to the cells by an indirect mechanism involving iron exchange into triacetylfusarinine C. In vitro ferric ion exchange was found to be rapid with triacetylfusarinine C. Gallium uptake rates comparable to those of iron were observed with the chelating …