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Changes In Quality Of Bone Mineral On Aging And In Disease, M. D. Grynpas, D. Holmyard

Scanning Microscopy

This paper reviews the changes in the quality of bone mineral with age and in disease. After a brief review of morphological changes with aging in mammalian bones, microradiography is compared to backscattered electron imaging and their use in bringing out subtle changes in bone mineralization outlined.

Changes in the quality of bone with disease is described using osteoporosis as an example. Chemical changes in the skeleton are then discussed and related to morphological changes. Finally, some examples of localized and generalized changes in bone mineral are given. This paper emphasizes that understanding the nature of the mineral phase in …

Quantitative Electron Probe X-Ray Microanalysis Of Lead-Sequestering Organelles In Earthworms: Technical Appraisal Of Air-Dried Smears And Freeze-Dried Cryosections, C. Winters, A. J. Morgan

Scanning Microscopy

Much of the current knowledge of Pb sequestration in earthworm chloragosome granules stems from quantitative and semi-quantitative Electron Probe X-ray Microanalysis (EPXMA) of unfixed, air-dried tissue smears. Fully quantitative EPXMA was used in this study to assess the validity of smearing by comparison with unfixed, freeze-dried cryosections. Samples were obtained from two earthworm species, Lumbricus rubellus (L.r.) and Dendrobaena rubida (D.r.) collected from a Pb and Zn polluted soil (confirmed by XRF). A number of conclusions emerged :- (a) the element compositions of whole worms, determined by EPXMA of sprayed microdroplets of wet-digested tissues, were different …

Quench Cooled Ice Crystal Imprint Size: A Micro-Method For Study Of Macromolecular Hydration, Ivan L. Cameron, Keithley E. Hunter, Gary D. Fullerton

Scanning Microscopy

Quench cooling (rate > 4000°C/sec) of biological specimens limits growth of ice crystals by processes different from slow cooling methods. Quench cooling in liquid propane cooled in liquid nitrogen induced ice crystal segregation compartments, as imaged by scanning transmission electron micrographs of freeze-dried cryosections of tissues and protein solutions. The observed imprints of ice crystals were relatively small and roughly spherical. The size of these ice crystal imprints increased with distance from the specimen/quenchant surface. Beyond a depth of 150 microns the size of the imprints was constant but differed among subcellular compartments. The size of the imprints was found to …

Low Temperature Scanning Electron Microscopy: Advantages And Applications, J. A. Sargent

Scanning Microscopy

Cryo-preparation of specimens for scanning electron microscopy can be completed within a few minutes. Chemical fixation and contact with solvents is avoided, levels of specimen hydration are maintained, low melting-point materials are stabilized, volume changes are minimized and internal structure can be revealed by freeze-fracture. Elements are not lost or substantially relocated prior to X-ray microanalysis and specimen luminescence is enhanced. The displacement of internal structure and material subject to X-ray microanalysis by the growth of ice crystals in hydrated samples can be minimized by adopting fast freezing methods designed to limit ice crystal growth. The technique enables a wide …

Theoretical Models Of Cyst Formation And Growth, Larry W. Welling, Dan J. Welling

Scanning Microscopy

Although the formation of fluid-filled, epithelial-lined cysts is a common event in a variety of tissues, the mechanisms involved are not well understood. Discussed here are means by which those mechanisms might be elucidated.

In general, there are too few data available for complete analysis of in vivo disease processes. It can be suggested only that epithelial proliferation and basement membrane growth are probably absolute requirements. Whether the forces for fluid accumulation precede or follow the stimuli for cell growth cannot be stated with certainty. On the other hand, in certain in vitro model systems the forces required to keep …

X-Ray Microanalysis Of Cartilage And Chondrocytes, Joanna Wroblewski, Anne-Marie Makower

Scanning Microscopy

The elemental content of differentiating chondrocytes in situ and in vitro was studied by X-ray microanalysis. The in situ studies were carried out on semi-thick cryosections and on thin sections of freeze-dried low-temperature vacuum-embedded rat rib growth plate. Resting, proliferative, and hypertrophic cells were analyzed separately. The in vitro studies were carried out on cultured cell fractions isolated from rat rib growth plate. The cell fractions were relatively homogeneous and contained resting - early proliferative, proliferative or hypertrophic cells, respectively. Data from cells in vitro are generally not comparable to data obtained from cells in situ. Chondrocytes in situ …

The Aging Cochlear Hair Cell, Matti Anniko

Scanning Microscopy

Specimens from the organ of Corti were taken from regions which appeared normal in the cytocochleogram as evaluated with the Nomarski optics. Ultrastructural, qualitative analyses of aging cochlear hair cells in the guinea pig showed, however, principally five distinct types of pathological changes: (1) disintegration of the cuticle; (2) increased amounts of intracellular lamellar structures and submembrane cisternae; (3) aggregations of electron optically dense particles; (4) lysosome-like structures; and (5) vesiculation of cytoplasm. Stereocilia remained intact also in cells where the cuticular plate showed severe degeneration. Outer hair cells showed more extensive cytological changes than inner hair cells. The ultrastructural …

The Retention Of Contractility Of Rabbit Myofibrils During Storage At 25 C, Makoto Nakamura, Michio Muguruma, Toshiyuki Fukazawa, Mamoru Yamaguchi

Food Structure

During postmortem storage of muscle at 25 degrees C, myofibrils retained the ability to contract for more than 400 hours under experimental conditions used to prevent bacterial contamination of the meat surface. During t his time, rapid breakdown of troponin T was observed. Storage beyond 500 hours resulted in rapid loss of contractility correlating with proteolysis of the heavy chain of myosin, an din this stage some granules were observed along the whole A-band within the sarcomere.

Proceedings From The 25th Annual Italian Cheese Seminar, Various Authors

Cheese Industry Conference

No abstract provided.

8th Biennial Cheese Industry Conference, Various Authors

Cheese Industry Conference

No abstract provided.

Nutrition Myths And Misinformation, Charlotte P. Brennand

Archived Food and Health Publications

Publication discusses common myths involving nutrition. It also discusses what information to trust and what information to question.

Ultrastructural And Textural Properties Of Restructured Beef Treated With A Bacterial Culture And Splenic Pulp, E. A. Elkhalifa, N. G. Marriot, R. L. Grayson, P. P. Graham, S. K. Perkins

Food Structure

Scanning electron microscopy (SEM), transmission electron microscopy (TEM) and Instron measurements were used to evaluate the effects of an Achromobacter iophagus culture {BC) and splenic pulp (SP) treatments one structural and textural properties of flaked and restructured beef steaks. Both treatments improved the textural character is tics of the product when conditioned at 35°C . Electron microscopy studies revealed that the bacterial culture treatment caused a greater effect than SP on the connective tissue elements, with a degradation of the endomysial sheath and sarcolemma. Treatment with splenic pulp produced an overall excessive disruption a t the Z-lines with little definition …

Pocket Gophers, Terry A. Messmer

Wildland Resources Faculty Publications

No abstract provided.

Effects Of Lignification, Cellulose Crystallinity And Enzyme Accessible Space On The Digestibility Of Plant Cell Wall Carbohydrates By The Ruminant, M. S. Kerley, G. C. Fahey Jr., J. M. Gould, E. L. Iannotti

Food Structure

Intrinsic characteristics of plant cell walls limiting susceptibility of structural carbohydrates to microbial attack in the ruminant's gastrointestinal tract are lignification of the cell wall, covalent bonding of phenolic acids to cell wall polysaccharides, the crystalline structure of cellulose and limited fibrolytic enzyme accessible space. The exact mechanism by which or degree to which each of these characteristics affect rate and/or extent of cell wall polysaccharide hydrolysis by gastrointestinal tract microbes is not well understood. Lignification and limited enzyme accessible space probably affect the extent of cell wall degradation by preventing contact between microbial enzymes and cell wall polysaccharides. Phenolic …

Evaluating Statistical Techniques For Predicting And Interpreting Forplan Results, United States Department Of Agriculture, Forest Service

Forestry

Two approaches using multiple linear regression for analyzing the effects of management constraints on an objective function in FORPLAN were tested on three National Forests. The two approaches, ordinary least squares regression and ordinary least squares using principal components, provide some degree of success in predicting objective function values, but very little information for interpreting the effects of the constraints on objective function values.

Selecting For Carcass Merit, Norris J. Stenquist

Archived Agriculture Publications

Today's consumers are asking for leaner, less-fatty beef. Much of the leaner beef now produced results from trimming excess fat before the product is sent to the consumer. For the future, the hope is to trim excess fat genetically by producing leaner, more heavily muscled beef cattle.

Fossil Butte Environmental Assessment, Develop Visitor, Administrative, And Maintenance Facilities, United States, Department Of The Interior, National Park Service

Environmental Assessments (WY)

The National Park Service (NPS) proposes to construct the first permanent visitor, administrative, and maintenance facilities at Fossil Butte National Monument since its Congressional establishment in 1972. Proposed facilities include: a 4,800-square-foot visitor center and administrative building on a 2-acre site approximately 1 mile north of Lincoln County Road 300; and a 2,000-square-foot maintenance building, materials storage yard, and seasonal-employee housing unit on a 5-acre site near the southwest corner of the monument. The employee housing unit is not part of this package and would be constructed at a later date if additional funding becomes available. Other proposed development includes …

Proposed Resource Management Plan And Final Environmental Impact Statement For The Cody Resource Area, Worland District, Wyoming, United States Department Of The Interior Bureau Of Land Management

Resource Management Plans (WY)

This document addresses alternatives for managing almost 1.1 million acres fo federal land and about 1.5 million acres of federal mineral estate administered by the Cody Resource Area, Worland District, Bureau of Land Management (see map 1). The plan focuses on three resource management issues relating to vegetative resources, special management area designations, and resource accessibility and manageability.

Pictures Past: A Centennial Celebration Of Utah State University, A J. Simmonds

Utah State University Faculty Monographs

This book does not pretend to present a comprehensive history of Utah State University. It cannot. It presents glimpses of that institution, glimpses that can be documented by the photographs preserved in University Archives. Entire areas of the University's past are ignored for no better reason than that no one had an available camera to record them; or that, once recorded, they never found their way into University Archives. The entire story is not told. That is the responsibility of another. I leave it to him. And if these pages are much concerned with the early years when the institution …

Homemade Nest Sites For Bluebirds, Terry A. Messmer, F. B. Lee

Wildland Resources Faculty Publications

No abstract provided.

Characterization Of Hypercontracted Fibers In Skeletal Muscle Of Domestic Turkey (Meleagris Gallopavo), A. Sosnicki, R. G. Cassens, D. R. Mcintyre, R. J. Vimini, M. L. Greaser

Food Structure

Several different muscles from a population of thirty Large White male turkeys were studied by histological and histochemical methods, and a high incidence of hypercontracted fibers was noted . The fibers were characterized in cross-section by an apparent swelling, a rounded rather than polygonal shape and a homogenous appearance. They were eosinophilic, positive to Gomori-trichrome and appeared to have an elevated lipid content. An unusual histochemical profile was noted. It was postulated that the hypercontracted fibers were real , not artifnctual, and resulted from some change occurring in the muscle.

Utah Science Vol. 49 No. 4, Winter 1988

Utah Science

94 FORESTS-OUR MOST IMPORTANT NATURAL RESOURCE R. F. Fisher In Utah? You bet This article explains why.

98 USING COMPUTER GRAPHICS TO ASSESS THE VISUAL IMPACT OF LIMB RUST IN PONDEROSA PINE F. A. Baker and D. Rabin Dead and dying trees benefit wildlife and can even enhance the scenery. New techniques will help manage forests to reflect these values.

103 SAVINGS POSSIBLE WITH CONSOLIDATION OF DAIRY PLANTS R. A. Christensen The economics of scale with larger. more centrally located dairy plants will reduce costs, even when energy costs increase by 100 percent

109 SILVICULTURE: SHAPING FUTURE FORESTS J. N. …

Tall Shrub Dynamics In Northern Minnesota Aspen And Conifer Forests, J.C. Balogh, D.F. Grigal

Aspen Bibliography

Tall shrubs are a dynamic component of upland forests in the Lake States (Balogh 1983, Dunn 1986, Ohmann and Ream 1971). They are an integral part of forest element cycles, competitors with overstory for growth and reproduction, essential to post-harvest site stabilization, and a source of wildlife cover and browse (Comerford and White 1977, Dunn 1986, Robinette 1972, Tappeiner 1971).

Diseases And Plant Population Biology, Nancy J. Huntly, R Inouye

Biology Faculty Publications

No abstract provided.

A Mass Mortality Of Fishes In Lake Titicaca (Peru-Bolivia) Associated With The Protozoan Parasite Ichthyophthirius Multifiliis, Wayne A. Wurtsbaugh, R. Alfaro

Watershed Sciences Faculty Publications

In December 1981 , an epizootic of the protozoan parasite Ichthyophthirius multifiliis killed an estimated 18 million killifish Orestias spp. in Lake Titicaca, a high-altitude tropical system. Of the dead fish collected, 93% were adult O. agassii, a commercially important species that is abundant in the littoral zone. Juvenile Orestias spp., pelagic species, and some other littoral zone fis hes were slightly affected. lchthyophthirius multifiliis was probably introduced with exotic fishes brought to Lake Titicaca in the 1940s and 1950s.

Human Health Effects Assays, Ronald C. Sims, J. L. Sims, R. R. Dupont

Biological Engineering Faculty Publications

Environmental health risk assessments based upon animal study results, epidemiology, quantitative structure activity relationships (QSARs), dose-response relationships, risk perception, and expert systems approaches, and the evaluation of mechanisms of carcinogenesis represent the focus for 1987. The term "biological risk assessment" was discussed by Clayson¹ to describe that part of risk assessment concerned with the relevance of specific animal results to the induction of human cancer. Guidelines for health assessment of suspect developmental toxicants addressed evaluation of data from animal testing studies.² Animal toxicity data on 200 chemicals and epidemiologic data on 30 chemicals was included within a data …

Great Salt Lake Interisland Diking: Water Quality Considerations, Executive Summary, Utah Water Research Laboratory, J. P. Riley, J. I. Blandamer, W. J. Doucette, R. R. Dupont, A. W. Grover, J. Herrick, J. M. Ihnat, J. E. Mclean, M. W. Nath, S. R. Rushforth, J. L. Sims, R. C. Sims, W. A. Wurtsbaugh

Reports

No abstract provided.

Great Salt Lake Interisland Diking: Water Quality Considerations, John I. Blandamer, William J. Doucette, Ryan R. Dupont, Anthony W. Grover, James Herrick, Jean M. Ihnat, Joan E. Mclean, Michael W. Nath, Samuel R. Rushforth, Judith L. Sims, Ronald C. Sims, Wayne A. Wurtsbaugh

Reports

No abstract provided.

Determinants Of Small Rodent Distribution And Abundance In A Shrub-Steppe Ecosystem: Influences Of Seeds, Ants And Shrubs, Linda Suzanne Broome

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

This study had two major objectives; firstly to test the hypothesis of ongoing competition for seeds between small rodents and harvester ants, Pogonomyrmex occidental is, in a cold desert ecosystem; secondly to elucidate how seed abundance and shrub cover influence the distribution and abundance of deer mice, Peromyscus maniculatus. The study area was a sagebrush (Artemisia tridentata) dominated shrubsteppe in southwestern Wyoming. Responses of the small mammal community to ant removal and food addition were assessed on replicated study plots between September 1981 and September 1983. Food addition and shrub removal manipulations were continued from September 1983 …

Fibroblast And Epidermal Cell-Type I Collagen Interactions: Cell Culture And Human Studies, Charles J. Doillon, Frederick H. Silver, Robert M. Olson, Chandrakala Y. Kamath, Richard A. Berg

Scanning Microscopy

Fibroblast and epidermal cell-type I collagen sponge interactions were studied in cell culture as well as in humans. In cell culture, fibroblasts were observed to migrate and proliferate throughout a type I collagen sponge containing either hyaluronic acid (HA) or fibronectin (FN). Fibroblasts accumulated in the center of the pores in sponges containing HA and appeared to surround themselves with newly synthesized extracellular matrix. In sponges containing FN, fibroblasts attached to and elongated along the collagen fibers of the sponge. In the absence of FN or HA protein synthesis of fibroblasts appeared to be inhibited by the presence of the …